Rh5CG350600

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0001964
Physical Location & Seq
Forward (+)
40387910 .. 40396817
8908 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG350600.1

Sequence Viewer

Length: 690 bp
ATGAGTTGTGTAAACCGATATTCTGTTGGAGAGTTCAAGGTAGCAATCTCCCACCCTATTGCAGCAGCATCCTCAGCCTTCAAAGTAGAACTGCTAGCGCTCAAAGCCGCTATGCATTTGATTTCGTCGCCGAACAATGATACCCATCGAGACGAAGTTGATTTTGAATTTTTGGGTAACAAGCAACCACCTTATACACTAAGTACAAATGTGATTTCAAATGGCAATTTACATCGAGAGCAACAAATTTCTCTATGGTTCGATCCTACAACAGATTTTCATTTATACCAAATTCTATGGAATAAACATCAACTGGTGTTATTTGTAGATGAGATCCCCATTAGGGTGTTTAAAAATGATCCAAGTATTAGACCTATTTTTCCAACAAAGCCTATGGAAATTCAAGGCACTATTTGGAATGCAACATGGGCTAAATCTGGATCGTTACCTGATTGGAGCCAAGGACCTTTCAAAGCATACTATCAAGGATTTGATATTTATGGGTGTCCCGCAACCAACTTGAACACTACAGAATGTTATGATTATAAGTATGCATGGAATGGAGAATATTATAAGTCTTTGAATCAAGTTCAAGAACAGGCATATGAAATTGTCAAGAGATACTACTTGAAATATAACTATTGTAGTGCAACCCATCCTTCACCCCCTGAATGCAAGATCAATAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_16 XET_C

Protein Analysis

229

Amino Acids

26.44

Weight (kDa)

6.37

Isoelectric Point (pI)

38.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 38 - 152 5.6e-31 Glycosyl hydrolases family 16
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 546, 573
AciI CCGC 2 cut(s) 108, 510
AclWI GGATC 4 cut(s) 257, 328, 353, 448
AcsI RAATTY 4 cut(s) 167, 246, 291, 399
AfaI GTAC 1 cut(s) 205
AfeI AGCGCT 1 cut(s) 99
AfiI CCNNNNNNNGG 1 cut(s) 343
Alw26I GTCTC 1 cut(s) 144
AlwI GGATC 4 cut(s) 257, 328, 353, 448
Aor51HI AGCGCT 1 cut(s) 99
ApeKI GCWGC 2 cut(s) 62, 65
ApoI RAATTY 4 cut(s) 167, 246, 291, 399
AspLEI GCGC 1 cut(s) 100
AspS9I GGNCC 1 cut(s) 464
AsuHPI GGTGA 1 cut(s) 654
AsuNHI GCTAGC 1 cut(s) 94
AvaII GGWCC 1 cut(s) 464
BbvCI CCTCAGC 1 cut(s) 73
BbvI GCAGC 2 cut(s) 74, 77
BccI CCATC 2 cut(s) 153, 663
BcoDI GTCTC 1 cut(s) 144
BfaI CTAG 1 cut(s) 95
BfmI CTRYAG 1 cut(s) 528
BfoI RGCGCY 1 cut(s) 101
BisI GCNGC 3 cut(s) 63, 66, 108
BlsI GCNGC 3 cut(s) 64, 67, 109
Bme18I GGWCC 1 cut(s) 464
BmgT120I GGNCC 1 cut(s) 464
BmiI GGNNCC 1 cut(s) 458
BmsI GCATC 1 cut(s) 77
BmtI GCTAGC 1 cut(s) 98
Bpu10I CCTNAGC 1 cut(s) 73
BsaBI GATNNNNATC 1 cut(s) 144
BsaJI CCNNGG 1 cut(s) 460
Bsc4I CCNNNNNNNGG 1 cut(s) 343
Bse1I ACTGG 1 cut(s) 318
Bse8I GATNNNNATC 1 cut(s) 144
BseDI CCNNGG 1 cut(s) 460
BseGI GGATG 2 cut(s) 68, 655
BseJI GATNNNNATC 1 cut(s) 144
BseLI CCNNNNNNNGG 1 cut(s) 343
BseMII CTCAG 1 cut(s) 87
BseNI ACTGG 1 cut(s) 318
BseXI GCAGC 2 cut(s) 74, 77
BslFI GGGAC 1 cut(s) 492
BslI CCNNNNNNNGG 1 cut(s) 343
BsmAI GTCTC 1 cut(s) 144
BsmBI CGTCTC 1 cut(s) 144
BsmFI GGGAC 1 cut(s) 492
BsmI GAATGC 2 cut(s) 424, 677
Bsp143I GATC 5 cut(s) 262, 333, 358, 440, 678
BspACI CCGC 2 cut(s) 108, 510
BspCNI CTCAG 1 cut(s) 86
BspLI GGNNCC 1 cut(s) 458
BspOI GCTAGC 1 cut(s) 98
BspPI GGATC 4 cut(s) 257, 328, 353, 448
BsrI ACTGG 1 cut(s) 318
BssECI CCNNGG 1 cut(s) 460
BssMI GATC 5 cut(s) 262, 333, 358, 440, 678
BssT1I CCWWGG 1 cut(s) 460
BstC8I GCNNGC 1 cut(s) 96
BstDEI CTNAG 2 cut(s) 73, 200
BstF5I GGATG 2 cut(s) 68, 655
BstH2I RGCGCY 1 cut(s) 101
BstHHI GCGC 1 cut(s) 100
BstKTI GATC 5 cut(s) 265, 336, 361, 443, 681
BstMAI GTCTC 1 cut(s) 144
BstMBI GATC 5 cut(s) 262, 333, 358, 440, 678
BstMWI GCNNNNNNNGC 3 cut(s) 74, 104, 428
BstSFI CTRYAG 1 cut(s) 528
BstV1I GCAGC 2 cut(s) 74, 77
BstX2I RGATCY 1 cut(s) 333
BstYI RGATCY 1 cut(s) 333
BtsCI GGATG 2 cut(s) 68, 655
Cac8I GCNNGC 1 cut(s) 96
CfoI GCGC 1 cut(s) 100
Cfr13I GGNCC 1 cut(s) 464
Csp6I GTAC 1 cut(s) 204
CviAII CATG 2 cut(s) 426, 555
CviJI RGCY 5 cut(s) 77, 107, 391, 431, 459
CviKI_1 RGCY 5 cut(s) 77, 107, 391, 431, 459
CviQI GTAC 1 cut(s) 204
DdeI CTNAG 2 cut(s) 73, 200
DpnI GATC 5 cut(s) 264, 335, 360, 442, 680
DpnII GATC 5 cut(s) 262, 333, 358, 440, 678
DraI TTTAAA 1 cut(s) 352
Eco130I CCWWGG 1 cut(s) 460
Eco47I GGWCC 1 cut(s) 464
Eco47III AGCGCT 1 cut(s) 99
EcoO109I RGGNCCY 1 cut(s) 464
EcoT14I CCWWGG 1 cut(s) 460
EcoT22I ATGCAT 2 cut(s) 117, 556
ErhI CCWWGG 1 cut(s) 460
Esp3I CGTCTC 1 cut(s) 144
FaeI CATG 2 cut(s) 429, 558
FaqI GGGAC 1 cut(s) 492
FatI CATG 2 cut(s) 425, 554
FauI CCCGC 1 cut(s) 517
FauNDI CATATG 1 cut(s) 604
Fnu4HI GCNGC 3 cut(s) 63, 66, 108
FokI GGATG 2 cut(s) 55, 642
Fsp4HI GCNGC 3 cut(s) 63, 66, 108
FspBI CTAG 1 cut(s) 95
GlaI GCGC 1 cut(s) 99
GluI GCNGC 3 cut(s) 63, 66, 108
HaeII RGCGCY 1 cut(s) 101
HhaI GCGC 1 cut(s) 100
Hin1II CATG 2 cut(s) 429, 558
Hin6I GCGC 1 cut(s) 98
HinP1I GCGC 1 cut(s) 98
HinfI GANTC 1 cut(s) 583
HphI GGTGA 1 cut(s) 654
Hpy166II GTNNAC 1 cut(s) 13
Hpy188III TCNNGA 5 cut(s) 149, 236, 438, 593, 616
Hpy8I GTNNAC 1 cut(s) 13
Hpy99I CGWCG 1 cut(s) 130
HpyAV CCTTC 2 cut(s) 88, 669
HpyCH4V TGCA 6 cut(s) 62, 115, 422, 554, 650, 675
HpyF10VI GCNNNNNNNGC 3 cut(s) 74, 104, 428
HpyF3I CTNAG 2 cut(s) 73, 200
Hsp92II CATG 2 cut(s) 429, 558
HspAI GCGC 1 cut(s) 98
Kzo9I GATC 5 cut(s) 262, 333, 358, 440, 678
LmnI GCTCC 1 cut(s) 456
LpnPI CCDG 5 cut(s) 299, 423, 462, 584, 681
Lsp1109I GCAGC 2 cut(s) 74, 77
LweI GCATC 1 cut(s) 77
MaeI CTAG 1 cut(s) 95
MaeIII GTNAC 2 cut(s) 176, 444
MalI GATC 5 cut(s) 264, 335, 360, 442, 680
MboI GATC 5 cut(s) 262, 333, 358, 440, 678
MflI RGATCY 1 cut(s) 333
MluCI AATT 7 cut(s) 167, 226, 246, 291, 399, 609, 685
MmeI TCCRAC 2 cut(s) 7, 407
MnlI CCTC 1 cut(s) 82
Mph1103I ATGCAT 2 cut(s) 117, 556
MseI TTAA 2 cut(s) 351, 688
MslI CAYNNNNRTG 1 cut(s) 344
Mva1269I GAATGC 2 cut(s) 424, 677
MwoI GCNNNNNNNGC 3 cut(s) 74, 104, 428
NdeI CATATG 1 cut(s) 604
NdeII GATC 5 cut(s) 262, 333, 358, 440, 678
NheI GCTAGC 1 cut(s) 94
NlaIII CATG 2 cut(s) 429, 558
NlaIV GGNNCC 1 cut(s) 458
NsiI ATGCAT 2 cut(s) 117, 556
PctI GAATGC 2 cut(s) 424, 677
PfeI GAWTC 1 cut(s) 583
PkrI GCNGC 3 cut(s) 64, 67, 109
PpuMI RGGWCCY 1 cut(s) 464
PsiI TTATAA 2 cut(s) 546, 573
Psp5II RGGWCCY 1 cut(s) 464
PspN4I GGNNCC 1 cut(s) 458
PspPI GGNCC 1 cut(s) 464
PspPPI RGGWCCY 1 cut(s) 464
PsuI RGATCY 1 cut(s) 333
RsaI GTAC 1 cut(s) 205
RsaNI GTAC 1 cut(s) 204
RseI CAYNNNNRTG 1 cut(s) 344
SaqAI TTAA 2 cut(s) 351, 688
SatI GCNGC 3 cut(s) 63, 66, 108
Sau3AI GATC 5 cut(s) 262, 333, 358, 440, 678
Sau96I GGNCC 1 cut(s) 464
SetI ASST 5 cut(s) 42, 193, 376, 451, 469
SfaNI GCATC 1 cut(s) 77
SfcI CTRYAG 1 cut(s) 528
SinI GGWCC 1 cut(s) 464
SmiMI CAYNNNNRTG 1 cut(s) 344
Sse9I AATT 7 cut(s) 167, 226, 246, 291, 399, 609, 685
SsiI CCGC 2 cut(s) 108, 510
SspI AATATT 1 cut(s) 569
SspMI CTAG 1 cut(s) 95
StyI CCWWGG 1 cut(s) 460
TaqI TCGA 3 cut(s) 148, 235, 261
TasI AATT 7 cut(s) 167, 226, 246, 291, 399, 609, 685
TatI WGTACW 1 cut(s) 203
TauI GCSGC 1 cut(s) 110
TfiI GAWTC 1 cut(s) 583
Tru1I TTAA 2 cut(s) 351, 688
Tru9I TTAA 2 cut(s) 351, 688
TseI GCWGC 2 cut(s) 62, 65
TspDTI ATGAA 2 cut(s) 269, 621
VpaK11BI GGWCC 1 cut(s) 464
XapI RAATTY 4 cut(s) 167, 246, 291, 399
XspI CTAG 1 cut(s) 95
Zsp2I ATGCAT 2 cut(s) 117, 556
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.