RchiOBHm_Chr6g0284461

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
Physical Location & Seq
Reverse (-)
47696697 .. 47697630
934 bp
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UTR
Exon/CDS
Intron
PRQ25509

Sequence Viewer

Length: 639 bp
ATGAGGATCAAGATACCACTAACAACAGAAGGTGATCGGCTGGATGAGATGGACTTTGAGTTCTTGGGTAATAGAGAAGGCAAGCCATTCGTATTGCAGACCAATGTGATTACTAATGGTGATAATAGAGAGCAAAGGATTCTTTTTTGGTTTGATCCCAATCAGATTTTCATGACTACAAAATTATTTGGAATCAGCATCAAATTTTTTTATGTCGACGATAAACCAATTAGGGTTTACAAGAACAATGAAAATATTAGGGTTCCCTACCCATCACGGCCAATGATAATAGACGCCAGCATATGGGATGGATCAAATTGGGAAACTGATGGAGGCCAAACAAAGATCGACTGGAACCATGCACTATTTAGAGCTTACTTCCAAGGGTTTGACATAAGTTCTGGCTGTACACTAGATAAGTCCAGGACCGATGTTAAGGAATGTTATGCATCCACATACTTGTGGAACCATCAGGAGTATTGGCAACTAAACTCAACCGAACAACAAGCATATGAGAAGGTGAGAAAGGAACAGATGAACTATGACTACTGTACTGATTCAACAAGGTTCCCTACACCTCCCCAGAGTGCAGTAGTAATACCTAATTTGTCGTGGAGTACGTACCAAAAAAGAGGCTAA

Protein Analysis

212

Amino Acids

25.07

Weight (kDa)

5.5

Isoelectric Point (pI)

35.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 8 - 119 2.6e-25 Glycosyl hydrolases family 16
XET_C PF06955 152 - 194 6.4e-13 Xyloglucan endo-transglycosylase (XET) C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 303
AccI GTMKAC 1 cut(s) 216
AclWI GGATC 3 cut(s) 14, 149, 319
AcoI YGGCCR 1 cut(s) 278
AcsI RAATTY 1 cut(s) 203
AcyI GRCGYC 1 cut(s) 294
AfaI GTAC 4 cut(s) 409, 553, 619, 623
AfiI CCNNNNNNNGG 1 cut(s) 303
AgsI TTSAA 1 cut(s) 561
AjnI CCWGG 1 cut(s) 422
AjuI GAANNNNNNNTTGG 2 cut(s) 152, 184
AloI GAACNNNNNNTCC 2 cut(s) 44, 76
AluBI AGCT 1 cut(s) 374
AluI AGCT 1 cut(s) 374
AlwI GGATC 3 cut(s) 14, 149, 319
AoxI GGCC 2 cut(s) 278, 334
ApoI RAATTY 1 cut(s) 203
AspS9I GGNCC 1 cut(s) 426
AsuHPI GGTGA 3 cut(s) 44, 131, 532
AvaII GGWCC 1 cut(s) 426
BccI CCATC 5 cut(s) 43, 280, 302, 323, 477
BceAI ACGGC 1 cut(s) 293
BcgI CGANNNNNNTGC 2 cut(s) 70, 104
BciT130I CCWGG 1 cut(s) 424
BfaI CTAG 1 cut(s) 413
Bme1390I CCNGG 1 cut(s) 424
Bme18I GGWCC 1 cut(s) 426
BmgT120I GGNCC 1 cut(s) 426
BmiI GGNNCC 4 cut(s) 264, 356, 467, 569
BmrFI CCNGG 1 cut(s) 424
BmsI GCATC 2 cut(s) 207, 458
BsaAI YACGTR 1 cut(s) 621
BsaBI GATNNNNATC 1 cut(s) 159
BsaHI GRCGYC 1 cut(s) 294
BsaJI CCNNGG 1 cut(s) 382
Bsc4I CCNNNNNNNGG 1 cut(s) 303
Bse1I ACTGG 1 cut(s) 356
Bse8I GATNNNNATC 1 cut(s) 159
BseBI CCWGG 1 cut(s) 424
BseDI CCNNGG 1 cut(s) 382
BseGI GGATG 3 cut(s) 49, 313, 449
BseJI GATNNNNATC 1 cut(s) 159
BseLI CCNNNNNNNGG 1 cut(s) 303
BseNI ACTGG 1 cut(s) 356
BsgI GTGCAG 1 cut(s) 609
BshFI GGCC 2 cut(s) 280, 336
BslI CCNNNNNNNGG 1 cut(s) 303
BsnI GGCC 2 cut(s) 280, 336
Bsp1407I TGTACA 1 cut(s) 407
Bsp143I GATC 5 cut(s) 6, 34, 154, 311, 345
BspANI GGCC 2 cut(s) 280, 336
BspHI TCATGA 1 cut(s) 171
BspLI GGNNCC 4 cut(s) 264, 356, 467, 569
BspPI GGATC 3 cut(s) 14, 149, 319
BsrGI TGTACA 1 cut(s) 407
BsrI ACTGG 1 cut(s) 356
BssECI CCNNGG 1 cut(s) 382
BssMI GATC 5 cut(s) 6, 34, 154, 311, 345
BssNI GRCGYC 1 cut(s) 294
BssT1I CCWWGG 1 cut(s) 382
Bst2UI CCWGG 1 cut(s) 424
Bst4CI ACNGT 1 cut(s) 551
BstACI GRCGYC 1 cut(s) 294
BstAUI TGTACA 1 cut(s) 407
BstBAI YACGTR 1 cut(s) 621
BstC8I GCNNGC 2 cut(s) 83, 298
BstF5I GGATG 3 cut(s) 49, 313, 449
BstKTI GATC 5 cut(s) 9, 37, 157, 314, 348
BstMBI GATC 5 cut(s) 6, 34, 154, 311, 345
BstNI CCWGG 1 cut(s) 424
BstSCI CCNGG 1 cut(s) 422
BstSNI TACGTA 1 cut(s) 621
BsuRI GGCC 2 cut(s) 280, 336
BtsCI GGATG 3 cut(s) 49, 313, 449
Cac8I GCNNGC 2 cut(s) 83, 298
CciI TCATGA 1 cut(s) 171
Cfr13I GGNCC 1 cut(s) 426
CseI GACGC 1 cut(s) 302
Csp6I GTAC 4 cut(s) 408, 552, 618, 622
CviAII CATG 2 cut(s) 172, 359
CviJI RGCY 7 cut(s) 40, 85, 280, 336, 374, 405, 636
CviKI_1 RGCY 7 cut(s) 40, 85, 280, 336, 374, 405, 636
CviQI GTAC 4 cut(s) 408, 552, 618, 622
DpnI GATC 5 cut(s) 8, 36, 156, 313, 347
DpnII GATC 5 cut(s) 6, 34, 154, 311, 345
EaeI YGGCCR 1 cut(s) 278
Eco105I TACGTA 1 cut(s) 621
Eco130I CCWWGG 1 cut(s) 382
Eco47I GGWCC 1 cut(s) 426
EcoRII CCWGG 1 cut(s) 422
EcoT14I CCWWGG 1 cut(s) 382
EcoT22I ATGCAT 1 cut(s) 451
ErhI CCWWGG 1 cut(s) 382
FaeI CATG 2 cut(s) 175, 362
FatI CATG 2 cut(s) 171, 358
FauNDI CATATG 2 cut(s) 302, 511
FblI GTMKAC 1 cut(s) 216
FokI GGATG 3 cut(s) 56, 320, 436
FspBI CTAG 1 cut(s) 413
HaeIII GGCC 2 cut(s) 280, 336
HgaI GACGC 1 cut(s) 302
Hin1I GRCGYC 1 cut(s) 294
Hin1II CATG 2 cut(s) 175, 362
HincII GTYRAC 1 cut(s) 217
HindII GTYRAC 1 cut(s) 217
HinfI GANTC 3 cut(s) 139, 192, 557
HphI GGTGA 3 cut(s) 44, 131, 532
Hpy166II GTNNAC 3 cut(s) 217, 238, 410
Hpy188I TCNGA 1 cut(s) 165
Hpy188III TCNNGA 3 cut(s) 10, 172, 473
Hpy8I GTNNAC 3 cut(s) 217, 238, 410
Hpy99I CGWCG 1 cut(s) 221
HpyAV CCTTC 3 cut(s) 23, 71, 511
HpyCH4III ACNGT 1 cut(s) 551
HpyCH4IV ACGT 1 cut(s) 620
HpyCH4V TGCA 4 cut(s) 97, 362, 449, 590
HpySE526I ACGT 1 cut(s) 620
Hsp92I GRCGYC 1 cut(s) 294
Hsp92II CATG 2 cut(s) 175, 362
Kzo9I GATC 5 cut(s) 6, 34, 154, 311, 345
LpnPI CCDG 8 cut(s) 26, 310, 337, 387, 409, 436, 458, 596
LweI GCATC 2 cut(s) 207, 458
MaeI CTAG 1 cut(s) 413
MaeII ACGT 1 cut(s) 620
MalI GATC 5 cut(s) 8, 36, 156, 313, 347
MboI GATC 5 cut(s) 6, 34, 154, 311, 345
MluCI AATT 5 cut(s) 182, 203, 228, 316, 604
MnlI CCTC 3 cut(s) 326, 588, 626
Mph1103I ATGCAT 1 cut(s) 451
MseI TTAA 1 cut(s) 435
MslI CAYNNNNRTG 1 cut(s) 460
MspR9I CCNGG 1 cut(s) 424
MvaI CCWGG 1 cut(s) 424
NdeI CATATG 2 cut(s) 302, 511
NdeII GATC 5 cut(s) 6, 34, 154, 311, 345
NlaIII CATG 2 cut(s) 175, 362
NlaIV GGNNCC 4 cut(s) 264, 356, 467, 569
NsiI ATGCAT 1 cut(s) 451
PagI TCATGA 1 cut(s) 171
PcsI WCGNNNNNNNCGW 1 cut(s) 617
PfeI GAWTC 3 cut(s) 139, 192, 557
PflMI CCANNNNNTGG 1 cut(s) 303
PfoI TCCNGGA 1 cut(s) 422
Ppu21I YACGTR 1 cut(s) 621
Psp6I CCWGG 1 cut(s) 422
PspGI CCWGG 1 cut(s) 422
PspN4I GGNNCC 4 cut(s) 264, 356, 467, 569
PspPI GGNCC 1 cut(s) 426
PsrI GAACNNNNNNTAC 2 cut(s) 530, 562
RsaI GTAC 4 cut(s) 409, 553, 619, 623
RsaNI GTAC 4 cut(s) 408, 552, 618, 622
RseI CAYNNNNRTG 1 cut(s) 460
SalI GTCGAC 1 cut(s) 215
SaqAI TTAA 1 cut(s) 435
Sau3AI GATC 5 cut(s) 6, 34, 154, 311, 345
Sau96I GGNCC 1 cut(s) 426
ScrFI CCNGG 1 cut(s) 424
SetI ASST 7 cut(s) 34, 376, 522, 569, 580, 604, 623
SfaNI GCATC 2 cut(s) 207, 458
SinI GGWCC 1 cut(s) 426
SmiMI CAYNNNNRTG 1 cut(s) 460
SnaBI TACGTA 1 cut(s) 621
Sse9I AATT 5 cut(s) 182, 203, 228, 316, 604
SspI AATATT 1 cut(s) 256
SspMI CTAG 1 cut(s) 413
StyD4I CCNGG 1 cut(s) 422
StyI CCWWGG 1 cut(s) 382
TaaI ACNGT 1 cut(s) 551
TaiI ACGT 1 cut(s) 623
TaqI TCGA 2 cut(s) 216, 348
TaqII GACCGA 1 cut(s) 443
TasI AATT 5 cut(s) 182, 203, 228, 316, 604
TatI WGTACW 2 cut(s) 407, 551
TfiI GAWTC 3 cut(s) 139, 192, 557
Tru1I TTAA 1 cut(s) 435
Tru9I TTAA 1 cut(s) 435
TspDTI ATGAA 3 cut(s) 160, 264, 551
Van91I CCANNNNNTGG 1 cut(s) 303
VpaK11BI GGWCC 1 cut(s) 426
XapI RAATTY 1 cut(s) 203
XmiI GTMKAC 1 cut(s) 216
XspI CTAG 1 cut(s) 413
Zsp2I ATGCAT 1 cut(s) 451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.