RchiOBHm_Chr5g0047321

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001964
Physical Location & Seq
Forward (+)
43698625 .. 43700697
2073 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ32525

Sequence Viewer

Length: 906 bp
ATGTTTCAAGCATATTATATGATAGTGCTTTTTGGTTTTGCTTTTTCATTACCCAATATGAGTGGTGCTGAACATGTACCCTTTGAGCTAAACTACAACATCACATATAGTTCACATCATACACAATTTCTTAACCAACATGAAGAGATTGTACTATCAATAGATAAACATTCAGGTTCTTTAATTTATTTTATTTTATTAGAGATACACTTACATGTTAATAAGTGTGGGAACACATTTACTACATCTCTAAGGAGATATGGTTCAGGATTATTTGGTATAAGACTAAAGCTCCCGAGTAATGATACTGGAGGAATCATTACAACCTTTTATTTGATTTCGTTGCCGAACAATGATACCCATCGAGATGAAGTTGATTTTGAATTTTTGGGTAACAAGCAACCACCCTATACGCTAAGTACAAATGTGATTTCAAATGGCAATTTACATCGAGAGCAACAAATTTCTCTATGGTTCGATCCTACAACATATTTTCATTTATACCAAATTCTATGGAACCAACATCAACTAGTGTTATTTGTAGATGAAATCCCCATTAGAGTGTTTAAAAATGATCCAAGTATCGGACCTATTTTTCCAACAAAGCCAATGGAAATTCAAGGCACTATTTGGAATGCAACATGGGCTAAACATGGATCGTTACCTGATTGGAGTCTAGGACCTTTCAAAGCATATTATCAAGGATTTGATATTGATGGGTGTCCCGCAACCAACTTGAACACTACAGAATGTCATGATTATAAGTATGCATGGAATGGAGAATATTATAAGTCTTTGAATCAAGTTCAAGAACATGCATATGAAATTGTCAAGAGATACTACTTGAAATATAACTATTGTACTGCAACCCATCCTTCACCCCCTGAATGCAAGATCAATAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_16 XET_C

Protein Analysis

301

Amino Acids

34.95

Weight (kDa)

6.08

Isoelectric Point (pI)

38.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 31 - 220 4.5e-40 Glycosyl hydrolases family 16
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 762, 789
AciI CCGC 1 cut(s) 726
AclWI GGATC 3 cut(s) 473, 569, 664
AcsI RAATTY 4 cut(s) 383, 462, 507, 615
AfaI GTAC 4 cut(s) 78, 153, 421, 862
AfiI CCNNNNNNNGG 1 cut(s) 584
AflIII ACRYGT 2 cut(s) 73, 214
AgsI TTSAA 9 cut(s) 8, 383, 435, 620, 688, 739, 799, 809, 847
AhlI ACTAGT 1 cut(s) 529
AloI GAACNNNNNNTCC 2 cut(s) 247, 279
AluBI AGCT 2 cut(s) 88, 292
AluI AGCT 2 cut(s) 88, 292
AlwI GGATC 3 cut(s) 473, 569, 664
Ama87I CYCGRG 1 cut(s) 295
ApoI RAATTY 4 cut(s) 383, 462, 507, 615
AspS9I GGNCC 2 cut(s) 587, 680
AsuHPI GGTGA 1 cut(s) 870
AvaI CYCGRG 1 cut(s) 295
AvaII GGWCC 2 cut(s) 587, 680
BarI GAAGNNNNNNTAC 2 cut(s) 135, 167
BccI CCATC 3 cut(s) 369, 710, 879
BcuI ACTAGT 1 cut(s) 529
BfaI CTAG 2 cut(s) 530, 677
BfmI CTRYAG 1 cut(s) 744
Bme18I GGWCC 2 cut(s) 587, 680
BmeT110I CYCGRG 1 cut(s) 295
BmgT120I GGNCC 2 cut(s) 587, 680
BmiI GGNNCC 1 cut(s) 518
BpmI CTGGAG 1 cut(s) 330
BsaBI GATNNNNATC 1 cut(s) 360
BsaXI ACNNNNNCTCC 4 cut(s) 247, 276, 277, 306
Bsc4I CCNNNNNNNGG 1 cut(s) 584
Bse1I ACTGG 1 cut(s) 313
Bse8I GATNNNNATC 1 cut(s) 360
BseGI GGATG 1 cut(s) 871
BseJI GATNNNNATC 1 cut(s) 360
BseLI CCNNNNNNNGG 1 cut(s) 584
BseNI ACTGG 1 cut(s) 313
BsiHKCI CYCGRG 1 cut(s) 295
BslFI GGGAC 1 cut(s) 708
BslI CCNNNNNNNGG 1 cut(s) 584
BsmFI GGGAC 1 cut(s) 708
BsmI GAATGC 2 cut(s) 640, 893
BsoBI CYCGRG 1 cut(s) 295
Bsp143I GATC 4 cut(s) 478, 574, 656, 894
BspACI CCGC 1 cut(s) 726
BspHI TCATGA 1 cut(s) 754
BspLI GGNNCC 1 cut(s) 518
BspPI GGATC 3 cut(s) 473, 569, 664
BsrI ACTGG 1 cut(s) 313
BssMI GATC 4 cut(s) 478, 574, 656, 894
Bst6I CTCTTC 1 cut(s) 138
BstDEI CTNAG 2 cut(s) 251, 416
BstF5I GGATG 1 cut(s) 871
BstKTI GATC 4 cut(s) 481, 577, 659, 897
BstMBI GATC 4 cut(s) 478, 574, 656, 894
BstMWI GCNNNNNNNGC 1 cut(s) 644
BstNSI RCATGY 3 cut(s) 77, 218, 818
BstSFI CTRYAG 1 cut(s) 744
BtsCI GGATG 1 cut(s) 871
CciI TCATGA 1 cut(s) 754
Cfr13I GGNCC 2 cut(s) 587, 680
Csp6I GTAC 4 cut(s) 77, 152, 420, 861
CspCI CAANNNNNGTGG 2 cut(s) 43, 78
CviAII CATG 8 cut(s) 74, 140, 215, 642, 653, 755, 771, 815
CviJI RGCY 4 cut(s) 88, 292, 607, 647
CviKI_1 RGCY 4 cut(s) 88, 292, 607, 647
CviQI GTAC 4 cut(s) 77, 152, 420, 861
DdeI CTNAG 2 cut(s) 251, 416
DpnI GATC 4 cut(s) 480, 576, 658, 896
DpnII GATC 4 cut(s) 478, 574, 656, 894
DraI TTTAAA 1 cut(s) 568
Eam1104I CTCTTC 1 cut(s) 138
EarI CTCTTC 1 cut(s) 138
Eco47I GGWCC 2 cut(s) 587, 680
Eco88I CYCGRG 1 cut(s) 295
EcoO109I RGGNCCY 1 cut(s) 680
EcoT22I ATGCAT 2 cut(s) 772, 820
FaeI CATG 8 cut(s) 77, 143, 218, 645, 656, 758, 774, 818
FaqI GGGAC 1 cut(s) 708
FatI CATG 8 cut(s) 73, 139, 214, 641, 652, 754, 770, 814
FauI CCCGC 1 cut(s) 733
FauNDI CATATG 1 cut(s) 820
FokI GGATG 1 cut(s) 858
FspBI CTAG 2 cut(s) 530, 677
GsuI CTGGAG 1 cut(s) 330
Hin1II CATG 8 cut(s) 77, 143, 218, 645, 656, 758, 774, 818
HinfI GANTC 3 cut(s) 315, 673, 799
HphI GGTGA 1 cut(s) 870
Hpy166II GTNNAC 1 cut(s) 113
Hpy188I TCNGA 1 cut(s) 587
Hpy188III TCNNGA 7 cut(s) 267, 295, 365, 452, 755, 809, 832
Hpy8I GTNNAC 1 cut(s) 113
HpyAV CCTTC 1 cut(s) 885
HpyCH4V TGCA 5 cut(s) 638, 770, 818, 866, 891
HpyF10VI GCNNNNNNNGC 1 cut(s) 644
HpyF3I CTNAG 2 cut(s) 251, 416
Hsp92II CATG 8 cut(s) 77, 143, 218, 645, 656, 758, 774, 818
Kzo9I GATC 4 cut(s) 478, 574, 656, 894
LmnI GCTCC 1 cut(s) 297
LpnPI CCDG 5 cut(s) 159, 252, 294, 678, 897
MaeI CTAG 2 cut(s) 530, 677
MaeIII GTNAC 2 cut(s) 392, 660
MalI GATC 4 cut(s) 480, 576, 658, 896
MboI GATC 4 cut(s) 478, 574, 656, 894
MboII GAAGA 1 cut(s) 155
MluCI AATT 9 cut(s) 125, 183, 383, 442, 462, 507, 615, 825, 901
MlyI GAGTC 1 cut(s) 682
MmeI TCCRAC 1 cut(s) 623
MnlI CCTC 1 cut(s) 305
Mph1103I ATGCAT 2 cut(s) 772, 820
MseI TTAA 5 cut(s) 132, 182, 219, 567, 904
MslI CAYNNNNRTG 4 cut(s) 213, 366, 560, 819
Mva1269I GAATGC 2 cut(s) 640, 893
MwoI GCNNNNNNNGC 1 cut(s) 644
NdeI CATATG 1 cut(s) 820
NdeII GATC 4 cut(s) 478, 574, 656, 894
NlaIII CATG 8 cut(s) 77, 143, 218, 645, 656, 758, 774, 818
NlaIV GGNNCC 1 cut(s) 518
NsiI ATGCAT 2 cut(s) 772, 820
NspI RCATGY 3 cut(s) 77, 218, 818
PagI TCATGA 1 cut(s) 754
PciI ACATGT 2 cut(s) 73, 214
PctI GAATGC 2 cut(s) 640, 893
PfeI GAWTC 2 cut(s) 315, 799
PleI GAGTC 1 cut(s) 681
PpsI GAGTC 1 cut(s) 681
PpuMI RGGWCCY 1 cut(s) 680
PscI ACATGT 2 cut(s) 73, 214
PsiI TTATAA 2 cut(s) 762, 789
Psp5II RGGWCCY 1 cut(s) 680
PspN4I GGNNCC 1 cut(s) 518
PspPI GGNCC 2 cut(s) 587, 680
PspPPI RGGWCCY 1 cut(s) 680
RsaI GTAC 4 cut(s) 78, 153, 421, 862
RsaNI GTAC 4 cut(s) 77, 152, 420, 861
RseI CAYNNNNRTG 4 cut(s) 213, 366, 560, 819
SaqAI TTAA 5 cut(s) 132, 182, 219, 567, 904
Sau3AI GATC 4 cut(s) 478, 574, 656, 894
Sau96I GGNCC 2 cut(s) 587, 680
SchI GAGTC 1 cut(s) 682
SetI ASST 7 cut(s) 90, 178, 294, 329, 592, 667, 685
SfcI CTRYAG 1 cut(s) 744
SinI GGWCC 2 cut(s) 587, 680
SmiMI CAYNNNNRTG 4 cut(s) 213, 366, 560, 819
SpeI ACTAGT 1 cut(s) 529
Sse9I AATT 9 cut(s) 125, 183, 383, 442, 462, 507, 615, 825, 901
SsiI CCGC 1 cut(s) 726
SspI AATATT 1 cut(s) 785
SspMI CTAG 2 cut(s) 530, 677
TaqI TCGA 3 cut(s) 364, 451, 477
TasI AATT 9 cut(s) 125, 183, 383, 442, 462, 507, 615, 825, 901
TatI WGTACW 3 cut(s) 151, 419, 860
TfiI GAWTC 2 cut(s) 315, 799
Tru1I TTAA 5 cut(s) 132, 182, 219, 567, 904
Tru9I TTAA 5 cut(s) 132, 182, 219, 567, 904
TspDTI ATGAA 6 cut(s) 36, 156, 384, 485, 561, 837
VpaK11BI GGWCC 2 cut(s) 587, 680
XapI RAATTY 4 cut(s) 383, 462, 507, 615
XceI RCATGY 3 cut(s) 77, 218, 818
XspI CTAG 2 cut(s) 530, 677
Zsp2I ATGCAT 2 cut(s) 772, 820
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.