Rh6DG271300

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0001964
Physical Location & Seq
Reverse (-)
46463861 .. 46465027
1167 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG271300.1

Sequence Viewer

Length: 609 bp
ATGATGCGGCTTTCTTTGGTCTTCTTTCTAGCACTTGTTGTAATGAAACATACAAGTGAAGCCGCTGATAATGTTAGCTTTGATGAAACCTACGTCGTTCGATGCGGAAATGATCATATCTCGAAGCTAGACCAAGGAAGAGAAAACCCGACATGCATGCTAGGATCGGGGTTTAATTCCAAGCATGGCTATGGTTCGGGATTCTTTGAAATGAAAATAAAGTTGGCGGCGAGACATTCTCCAGGAGTTGTCGCCACATTTTATCTAAGTTCACATCCAGATAGTAAGCCTGGAAATCATGATGAGCTAGACTTTGAGTTCTTGGGCACAGATGGACCTGTATACTCTTTACAAACAAATATTTTCGCAAATGACAATGGGAAGAGAGAGCAAAGACTTCATCTTTGGTTTGATCCCACAAAGAATTTTCTTAAGTATGGAATCCTATGGAACCATCGCCAAGTAGTCTTTTTTGTGGACAACATCCCCATTAGAGTGTTCAAGAACAATACAAGGTTGGCTGCAAGGTACCCTTCACAACAGATGTCTGTGGAGGGAAGCATATGGAATATATGGAGAATCTTGGGCATCAAGTGGGAAAAAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_16 XET_C

Protein Analysis

202

Amino Acids

23.22

Weight (kDa)

8.51

Isoelectric Point (pI)

31.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 53 - 190 2.1e-41 Glycosyl hydrolases family 16
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 528
AccB1I GGYRCC 1 cut(s) 528
AccI GTMKAC 1 cut(s) 342
AciI CCGC 4 cut(s) 7, 63, 105, 227
AclWI GGATC 2 cut(s) 172, 407
AcsI RAATTY 1 cut(s) 424
AfaI GTAC 1 cut(s) 530
AflII CTTAAG 1 cut(s) 431
AgsI TTSAA 2 cut(s) 209, 502
AjnI CCWGG 2 cut(s) 241, 289
AjuI GAANNNNNNNTTGG 2 cut(s) 206, 238
AluBI AGCT 3 cut(s) 78, 127, 307
AluI AGCT 3 cut(s) 78, 127, 307
Alw26I GTCTC 1 cut(s) 226
AlwI GGATC 2 cut(s) 172, 407
ApeKI GCWGC 1 cut(s) 521
ApoI RAATTY 1 cut(s) 424
ArsI GACNNNNNNTTYG 2 cut(s) 387, 419
Asp718I GGTACC 1 cut(s) 528
AspS9I GGNCC 1 cut(s) 335
AvaII GGWCC 1 cut(s) 335
BaeGI GKGCMC 1 cut(s) 329
BanI GGYRCC 1 cut(s) 528
BbsI GAAGAC 1 cut(s) 13
BbvI GCAGC 1 cut(s) 508
BccI CCATC 2 cut(s) 326, 462
BcgI CGANNNNNNTGC 2 cut(s) 139, 173
BciT130I CCWGG 2 cut(s) 243, 291
BclI TGATCA 1 cut(s) 112
BcoDI GTCTC 1 cut(s) 226
BfaI CTAG 4 cut(s) 29, 128, 161, 308
BfrI CTTAAG 1 cut(s) 431
BisI GCNGC 4 cut(s) 8, 63, 228, 522
BlsI GCNGC 4 cut(s) 9, 64, 229, 523
Bme1390I CCNGG 2 cut(s) 243, 291
Bme18I GGWCC 1 cut(s) 335
BmgT120I GGNCC 1 cut(s) 335
BmiI GGNNCC 2 cut(s) 452, 530
BmrFI CCNGG 2 cut(s) 243, 291
BmsI GCATC 2 cut(s) 92, 597
BpiI GAAGAC 1 cut(s) 13
BplI GAGNNNNNCTC 2 cut(s) 223, 255
BpmI CTGGAG 1 cut(s) 225
BsaJI CCNNGG 1 cut(s) 133
BseBI CCWGG 2 cut(s) 243, 291
BseDI CCNNGG 1 cut(s) 133
BseGI GGATG 2 cut(s) 274, 483
BseSI GKGCMC 1 cut(s) 329
BseXI GCAGC 1 cut(s) 508
BshNI GGYRCC 1 cut(s) 528
BsmAI GTCTC 1 cut(s) 226
Bsp1286I GDGCHC 1 cut(s) 329
Bsp143I GATC 3 cut(s) 112, 164, 412
BspACI CCGC 4 cut(s) 7, 63, 105, 227
BspHI TCATGA 1 cut(s) 298
BspLI GGNNCC 2 cut(s) 452, 530
BspPI GGATC 2 cut(s) 172, 407
BspT107I GGYRCC 1 cut(s) 528
BspTI CTTAAG 1 cut(s) 431
BssECI CCNNGG 1 cut(s) 133
BssMI GATC 3 cut(s) 112, 164, 412
BssNAI GTATAC 1 cut(s) 343
BssT1I CCWWGG 1 cut(s) 133
Bst1107I GTATAC 1 cut(s) 343
Bst2UI CCWGG 2 cut(s) 243, 291
Bst6I CTCTTC 2 cut(s) 133, 377
BstAFI CTTAAG 1 cut(s) 431
BstC8I GCNNGC 1 cut(s) 158
BstDEI CTNAG 1 cut(s) 266
BstF5I GGATG 2 cut(s) 274, 483
BstKTI GATC 3 cut(s) 115, 167, 415
BstMAI GTCTC 1 cut(s) 226
BstMBI GATC 3 cut(s) 112, 164, 412
BstNI CCWGG 2 cut(s) 243, 291
BstNSI RCATGY 2 cut(s) 156, 160
BstSCI CCNGG 2 cut(s) 241, 289
BstSLI GKGCMC 1 cut(s) 329
BstV1I GCAGC 1 cut(s) 508
BstV2I GAAGAC 1 cut(s) 13
BstZ17I GTATAC 1 cut(s) 343
BtgZI GCGATG 1 cut(s) 440
BtsCI GGATG 2 cut(s) 274, 483
Cac8I GCNNGC 1 cut(s) 158
CciI TCATGA 1 cut(s) 298
Cfr13I GGNCC 1 cut(s) 335
Csp6I GTAC 1 cut(s) 529
CviAII CATG 4 cut(s) 153, 157, 185, 299
CviJI RGCY 8 cut(s) 10, 62, 78, 127, 189, 289, 307, 521
CviKI_1 RGCY 8 cut(s) 10, 62, 78, 127, 189, 289, 307, 521
CviQI GTAC 1 cut(s) 529
DdeI CTNAG 1 cut(s) 266
DpnI GATC 3 cut(s) 114, 166, 414
DpnII GATC 3 cut(s) 112, 164, 412
Eam1104I CTCTTC 2 cut(s) 133, 377
EarI CTCTTC 2 cut(s) 133, 377
Eco130I CCWWGG 1 cut(s) 133
Eco47I GGWCC 1 cut(s) 335
EcoRII CCWGG 2 cut(s) 241, 289
EcoT14I CCWWGG 1 cut(s) 133
EcoT22I ATGCAT 1 cut(s) 158
ErhI CCWWGG 1 cut(s) 133
FaeI CATG 4 cut(s) 156, 160, 188, 302
FalI AAGNNNNNCTT 2 cut(s) 517, 549
FatI CATG 4 cut(s) 152, 156, 184, 298
FauNDI CATATG 1 cut(s) 563
FbaI TGATCA 1 cut(s) 112
FblI GTMKAC 1 cut(s) 342
Fnu4HI GCNGC 4 cut(s) 8, 63, 228, 522
FokI GGATG 2 cut(s) 261, 470
Fsp4HI GCNGC 4 cut(s) 8, 63, 228, 522
FspBI CTAG 4 cut(s) 29, 128, 161, 308
GluI GCNGC 4 cut(s) 8, 63, 228, 522
GsuI CTGGAG 1 cut(s) 225
Hin1II CATG 4 cut(s) 156, 160, 188, 302
HinfI GANTC 3 cut(s) 201, 441, 579
Hpy166II GTNNAC 3 cut(s) 272, 343, 478
Hpy188III TCNNGA 5 cut(s) 121, 198, 278, 299, 502
Hpy8I GTNNAC 3 cut(s) 272, 343, 478
Hpy99I CGWCG 1 cut(s) 98
HpyAV CCTTC 1 cut(s) 543
HpyCH4IV ACGT 1 cut(s) 93
HpyCH4V TGCA 2 cut(s) 156, 524
HpyF3I CTNAG 1 cut(s) 266
HpySE526I ACGT 1 cut(s) 93
Hsp92II CATG 4 cut(s) 156, 160, 188, 302
KpnI GGTACC 1 cut(s) 532
Ksp22I TGATCA 1 cut(s) 112
Kzo9I GATC 3 cut(s) 112, 164, 412
LpnPI CCDG 6 cut(s) 228, 255, 276, 291, 303, 351
Lsp1109I GCAGC 1 cut(s) 508
LweI GCATC 2 cut(s) 92, 597
MaeI CTAG 4 cut(s) 29, 128, 161, 308
MaeII ACGT 1 cut(s) 93
MalI GATC 3 cut(s) 114, 166, 414
MboI GATC 3 cut(s) 112, 164, 412
MboII GAAGA 3 cut(s) 13, 150, 394
MhlI GDGCHC 1 cut(s) 329
MluCI AATT 2 cut(s) 175, 424
MnlI CCTC 1 cut(s) 547
Mph1103I ATGCAT 1 cut(s) 158
MseI TTAA 3 cut(s) 174, 432, 607
MslI CAYNNNNRTG 3 cut(s) 54, 189, 494
MspA1I CMGCKG 1 cut(s) 65
MspCI CTTAAG 1 cut(s) 431
MspR9I CCNGG 2 cut(s) 243, 291
MvaI CCWGG 2 cut(s) 243, 291
NdeI CATATG 1 cut(s) 563
NdeII GATC 3 cut(s) 112, 164, 412
NlaIII CATG 4 cut(s) 156, 160, 188, 302
NlaIV GGNNCC 2 cut(s) 452, 530
NsiI ATGCAT 1 cut(s) 158
NspI RCATGY 2 cut(s) 156, 160
PaeI GCATGC 1 cut(s) 160
PagI TCATGA 1 cut(s) 298
PfeI GAWTC 3 cut(s) 201, 441, 579
PfoI TCCNGGA 1 cut(s) 241
PkrI GCNGC 4 cut(s) 9, 64, 229, 523
Psp6I CCWGG 2 cut(s) 241, 289
PspGI CCWGG 2 cut(s) 241, 289
PspN4I GGNNCC 2 cut(s) 452, 530
PspPI GGNCC 1 cut(s) 335
RsaI GTAC 1 cut(s) 530
RsaNI GTAC 1 cut(s) 529
RseI CAYNNNNRTG 3 cut(s) 54, 189, 494
SaqAI TTAA 3 cut(s) 174, 432, 607
SatI GCNGC 4 cut(s) 8, 63, 228, 522
Sau3AI GATC 3 cut(s) 112, 164, 412
Sau96I GGNCC 1 cut(s) 335
ScrFI CCNGG 2 cut(s) 243, 291
SduI GDGCHC 1 cut(s) 329
SetI ASST 8 cut(s) 80, 92, 96, 129, 309, 340, 518, 530
SfaNI GCATC 2 cut(s) 92, 597
SinI GGWCC 1 cut(s) 335
SmiMI CAYNNNNRTG 3 cut(s) 54, 189, 494
SmlI CTYRAG 1 cut(s) 431
SmoI CTYRAG 1 cut(s) 431
SphI GCATGC 1 cut(s) 160
Sse9I AATT 2 cut(s) 175, 424
SsiI CCGC 4 cut(s) 7, 63, 105, 227
SspI AATATT 1 cut(s) 361
SspMI CTAG 4 cut(s) 29, 128, 161, 308
StyD4I CCNGG 2 cut(s) 241, 289
StyI CCWWGG 1 cut(s) 133
TaiI ACGT 1 cut(s) 96
TaqI TCGA 2 cut(s) 100, 122
TasI AATT 2 cut(s) 175, 424
TauI GCSGC 3 cut(s) 10, 65, 230
TfiI GAWTC 3 cut(s) 201, 441, 579
Tru1I TTAA 3 cut(s) 174, 432, 607
Tru9I TTAA 3 cut(s) 174, 432, 607
TseI GCWGC 1 cut(s) 521
TspDTI ATGAA 4 cut(s) 59, 99, 227, 389
Vha464I CTTAAG 1 cut(s) 431
VpaK11BI GGWCC 1 cut(s) 335
XapI RAATTY 1 cut(s) 424
XceI RCATGY 2 cut(s) 156, 160
XmiI GTMKAC 1 cut(s) 342
XspI CTAG 4 cut(s) 29, 128, 161, 308
Zsp2I ATGCAT 1 cut(s) 158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.