Rh5DG334400

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0001964
Physical Location & Seq
Forward (+)
44253438 .. 44292003
38566 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG334400.1

Sequence Viewer

Length: 822 bp
ATGGTCTTAGATAAACCTAATCCCCTTAATCCTTGCTGCAACCTCTTCCAACATATTATGATCAAGATCTGCCATCACGTGATAAGCGGAGGGCCAAGGCGGGGCGACCTGCGGTGGCGCCGACGGGAAGAAGCCGGAGCAAGCTGCGGAACTGGATTTAGATCTCTAAGGAGATATGGTTCAGGATTATTTGGTATAAGACTAAAGCTCCCGAGTAATGATACTGGAGGAATCATTACAACCTTTTATTTGATTTCGTTGCCGAACAATGATACCCATCGAGATGAAGTTGATTTTGAATTTTTGGGTAACAAGCAACCACCCTATACGCTAAGTACAAATGTGATTTCAAATGGCAATTTACATCGAGAGCAACAAATTTCTCTATGGTTCGATCCTACAACATATTTTCATTTATACCAAATTCTATGGAACCAACATCAACTAGTGTTATTTGTAGATGAAATCCCCATTAGAGTGTTTAAAAATGATCCAAGTATCGGACCTATTTTTCCAACAAAGCCAATGGAAATTCAAGGCACTATTTGGAATGCAACATGGGCTAAACATGGATCGTTACCTGATTGGAGTCTAGGACCTTTCAAAGCATATTATCAAGGATTTGATATTGATGGGTGTCCCGCAACCAACTTGAACACTACAGAATGTCATGATTATAAGTATGCATGGAATGGAGAATATTATAAGTCTTTGAATCAAGTTCAAGAACATGCATATGAAATTGTCAAGAGATACTACTTGAAATATAACTATTGTACTGCAACCCATCCTTCACCCCCTGAATGCAAGATCAATAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_16 XET_C

Protein Analysis

273

Amino Acids

31.58

Weight (kDa)

8.07

Isoelectric Point (pI)

44.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 48 - 192 4.9e-39 Glycosyl hydrolases family 16
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 678, 705
Acc36I ACCTGC 1 cut(s) 117
AccB1I GGYRCC 1 cut(s) 117
AciI CCGC 5 cut(s) 87, 100, 112, 147, 642
AclWI GGATC 3 cut(s) 389, 485, 580
AcsI RAATTY 4 cut(s) 299, 378, 423, 531
AcvI CACGTG 1 cut(s) 79
AcyI GRCGYC 1 cut(s) 118
AfaI GTAC 2 cut(s) 337, 778
AfiI CCNNNNNNNGG 2 cut(s) 101, 500
AgsI TTSAA 8 cut(s) 299, 351, 536, 604, 655, 715, 725, 763
AhlI ACTAGT 1 cut(s) 445
AloI GAACNNNNNNTCC 2 cut(s) 163, 195
AluBI AGCT 2 cut(s) 144, 208
AluI AGCT 2 cut(s) 144, 208
AlwI GGATC 3 cut(s) 389, 485, 580
Ama87I CYCGRG 1 cut(s) 211
AoxI GGCC 1 cut(s) 92
ApeKI GCWGC 2 cut(s) 36, 144
ApoI RAATTY 4 cut(s) 299, 378, 423, 531
AspLEI GCGC 1 cut(s) 120
AspS9I GGNCC 3 cut(s) 92, 503, 596
AsuHPI GGTGA 1 cut(s) 786
AvaI CYCGRG 1 cut(s) 211
AvaII GGWCC 2 cut(s) 503, 596
BanI GGYRCC 1 cut(s) 117
BbrPI CACGTG 1 cut(s) 79
BbvI GCAGC 2 cut(s) 23, 131
BccI CCATC 4 cut(s) 81, 285, 626, 795
BclI TGATCA 1 cut(s) 60
BcuI ACTAGT 1 cut(s) 445
BfaI CTAG 2 cut(s) 446, 593
BfmI CTRYAG 1 cut(s) 660
BfoI RGCGCY 1 cut(s) 121
BfuAI ACCTGC 1 cut(s) 117
BglII AGATCT 2 cut(s) 66, 161
BisI GCNGC 2 cut(s) 37, 145
BlsI GCNGC 2 cut(s) 38, 146
Bme18I GGWCC 2 cut(s) 503, 596
BmeT110I CYCGRG 1 cut(s) 211
BmgT120I GGNCC 3 cut(s) 92, 503, 596
BmiI GGNNCC 2 cut(s) 119, 434
BpmI CTGGAG 1 cut(s) 246
BsaAI YACGTR 1 cut(s) 79
BsaBI GATNNNNATC 3 cut(s) 65, 160, 276
BsaHI GRCGYC 1 cut(s) 118
BsaJI CCNNGG 1 cut(s) 95
BsaXI ACNNNNNCTCC 4 cut(s) 163, 192, 193, 222
Bsc4I CCNNNNNNNGG 2 cut(s) 101, 500
Bse1I ACTGG 2 cut(s) 157, 229
Bse8I GATNNNNATC 3 cut(s) 65, 160, 276
BseDI CCNNGG 1 cut(s) 95
BseGI GGATG 1 cut(s) 787
BseJI GATNNNNATC 3 cut(s) 65, 160, 276
BseLI CCNNNNNNNGG 2 cut(s) 101, 500
BseNI ACTGG 2 cut(s) 157, 229
BseXI GCAGC 2 cut(s) 23, 131
BshFI GGCC 1 cut(s) 94
BshNI GGYRCC 1 cut(s) 117
BsiHKCI CYCGRG 1 cut(s) 211
BsiSI CCGG 1 cut(s) 135
BslFI GGGAC 1 cut(s) 624
BslI CCNNNNNNNGG 2 cut(s) 101, 500
BsmFI GGGAC 1 cut(s) 624
BsmI GAATGC 2 cut(s) 556, 809
BsnI GGCC 1 cut(s) 94
BsoBI CYCGRG 1 cut(s) 211
Bsp143I GATC 7 cut(s) 60, 66, 161, 394, 490, 572, 810
BspACI CCGC 5 cut(s) 87, 100, 112, 147, 642
BspANI GGCC 1 cut(s) 94
BspHI TCATGA 1 cut(s) 670
BspLI GGNNCC 2 cut(s) 119, 434
BspMI ACCTGC 1 cut(s) 117
BspPI GGATC 3 cut(s) 389, 485, 580
BspT107I GGYRCC 1 cut(s) 117
BsrI ACTGG 2 cut(s) 157, 229
BssECI CCNNGG 1 cut(s) 95
BssMI GATC 7 cut(s) 60, 66, 161, 394, 490, 572, 810
BssNI GRCGYC 1 cut(s) 118
BssT1I CCWWGG 1 cut(s) 95
Bst6I CTCTTC 1 cut(s) 50
BstACI GRCGYC 1 cut(s) 118
BstBAI YACGTR 1 cut(s) 79
BstC8I GCNNGC 1 cut(s) 142
BstDEI CTNAG 3 cut(s) 7, 167, 332
BstF5I GGATG 1 cut(s) 787
BstH2I RGCGCY 1 cut(s) 121
BstHHI GCGC 1 cut(s) 120
BstKTI GATC 7 cut(s) 63, 69, 164, 397, 493, 575, 813
BstMBI GATC 7 cut(s) 60, 66, 161, 394, 490, 572, 810
BstMWI GCNNNNNNNGC 1 cut(s) 560
BstNSI RCATGY 1 cut(s) 734
BstSFI CTRYAG 1 cut(s) 660
BstV1I GCAGC 2 cut(s) 23, 131
BstX2I RGATCY 2 cut(s) 66, 161
BstYI RGATCY 2 cut(s) 66, 161
BsuRI GGCC 1 cut(s) 94
BtsCI GGATG 1 cut(s) 787
BveI ACCTGC 1 cut(s) 117
Cac8I GCNNGC 1 cut(s) 142
CciI TCATGA 1 cut(s) 670
CfoI GCGC 1 cut(s) 120
Cfr13I GGNCC 3 cut(s) 92, 503, 596
Csp6I GTAC 2 cut(s) 336, 777
CviAII CATG 5 cut(s) 558, 569, 671, 687, 731
CviJI RGCY 6 cut(s) 94, 134, 144, 208, 523, 563
CviKI_1 RGCY 6 cut(s) 94, 134, 144, 208, 523, 563
CviQI GTAC 2 cut(s) 336, 777
DdeI CTNAG 3 cut(s) 7, 167, 332
DinI GGCGCC 1 cut(s) 119
DpnI GATC 7 cut(s) 62, 68, 163, 396, 492, 574, 812
DpnII GATC 7 cut(s) 60, 66, 161, 394, 490, 572, 810
DraI TTTAAA 1 cut(s) 484
Eam1104I CTCTTC 1 cut(s) 50
EarI CTCTTC 1 cut(s) 50
Eco130I CCWWGG 1 cut(s) 95
Eco47I GGWCC 2 cut(s) 503, 596
Eco72I CACGTG 1 cut(s) 79
Eco88I CYCGRG 1 cut(s) 211
EcoO109I RGGNCCY 1 cut(s) 596
EcoT14I CCWWGG 1 cut(s) 95
EcoT22I ATGCAT 2 cut(s) 688, 736
EgeI GGCGCC 1 cut(s) 119
EheI GGCGCC 1 cut(s) 119
ErhI CCWWGG 1 cut(s) 95
FaeI CATG 5 cut(s) 561, 572, 674, 690, 734
FaqI GGGAC 1 cut(s) 624
FatI CATG 5 cut(s) 557, 568, 670, 686, 730
FauI CCCGC 2 cut(s) 93, 649
FauNDI CATATG 1 cut(s) 736
FbaI TGATCA 1 cut(s) 60
Fnu4HI GCNGC 2 cut(s) 37, 145
FokI GGATG 1 cut(s) 774
Fsp4HI GCNGC 2 cut(s) 37, 145
FspBI CTAG 2 cut(s) 446, 593
GlaI GCGC 1 cut(s) 119
GluI GCNGC 2 cut(s) 37, 145
GsuI CTGGAG 1 cut(s) 246
HaeII RGCGCY 1 cut(s) 121
HaeIII GGCC 1 cut(s) 94
HapII CCGG 1 cut(s) 135
HhaI GCGC 1 cut(s) 120
Hin1I GRCGYC 1 cut(s) 118
Hin1II CATG 5 cut(s) 561, 572, 674, 690, 734
Hin6I GCGC 1 cut(s) 118
HinP1I GCGC 1 cut(s) 118
HinfI GANTC 3 cut(s) 231, 589, 715
HpaII CCGG 1 cut(s) 135
HphI GGTGA 1 cut(s) 786
Hpy188I TCNGA 1 cut(s) 503
Hpy188III TCNNGA 8 cut(s) 64, 183, 211, 281, 368, 671, 725, 748
Hpy99I CGWCG 1 cut(s) 126
HpyAV CCTTC 1 cut(s) 801
HpyCH4IV ACGT 1 cut(s) 78
HpyCH4V TGCA 6 cut(s) 39, 554, 686, 734, 782, 807
HpyF10VI GCNNNNNNNGC 1 cut(s) 560
HpyF3I CTNAG 3 cut(s) 7, 167, 332
HpySE526I ACGT 1 cut(s) 78
Hsp92I GRCGYC 1 cut(s) 118
Hsp92II CATG 5 cut(s) 561, 572, 674, 690, 734
HspAI GCGC 1 cut(s) 118
KasI GGCGCC 1 cut(s) 117
Ksp22I TGATCA 1 cut(s) 60
Kzo9I GATC 7 cut(s) 60, 66, 161, 394, 490, 572, 810
LmnI GCTCC 2 cut(s) 137, 213
LpnPI CCDG 7 cut(s) 122, 138, 148, 168, 210, 594, 813
Lsp1109I GCAGC 2 cut(s) 23, 131
MaeI CTAG 2 cut(s) 446, 593
MaeII ACGT 1 cut(s) 78
MaeIII GTNAC 2 cut(s) 308, 576
MalI GATC 7 cut(s) 62, 68, 163, 396, 492, 574, 812
MboI GATC 7 cut(s) 60, 66, 161, 394, 490, 572, 810
MboII GAAGA 2 cut(s) 37, 140
MflI RGATCY 2 cut(s) 66, 161
MluCI AATT 7 cut(s) 299, 358, 378, 423, 531, 741, 817
Mly113I GGCGCC 1 cut(s) 118
MlyI GAGTC 1 cut(s) 598
MmeI TCCRAC 2 cut(s) 73, 539
MnlI CCTC 3 cut(s) 53, 83, 221
Mph1103I ATGCAT 2 cut(s) 688, 736
MseI TTAA 3 cut(s) 27, 483, 820
MslI CAYNNNNRTG 3 cut(s) 282, 476, 735
MspI CCGG 1 cut(s) 135
Mva1269I GAATGC 2 cut(s) 556, 809
MwoI GCNNNNNNNGC 1 cut(s) 560
NarI GGCGCC 1 cut(s) 118
NdeI CATATG 1 cut(s) 736
NdeII GATC 7 cut(s) 60, 66, 161, 394, 490, 572, 810
NlaIII CATG 5 cut(s) 561, 572, 674, 690, 734
NlaIV GGNNCC 2 cut(s) 119, 434
NsiI ATGCAT 2 cut(s) 688, 736
NspI RCATGY 1 cut(s) 734
PagI TCATGA 1 cut(s) 670
PctI GAATGC 2 cut(s) 556, 809
PfeI GAWTC 2 cut(s) 231, 715
PkrI GCNGC 2 cut(s) 38, 146
PleI GAGTC 1 cut(s) 597
PluTI GGCGCC 1 cut(s) 121
PmaCI CACGTG 1 cut(s) 79
PmlI CACGTG 1 cut(s) 79
PpsI GAGTC 1 cut(s) 597
Ppu21I YACGTR 1 cut(s) 79
PpuMI RGGWCCY 1 cut(s) 596
PsiI TTATAA 2 cut(s) 678, 705
Psp5II RGGWCCY 1 cut(s) 596
PspCI CACGTG 1 cut(s) 79
PspN4I GGNNCC 2 cut(s) 119, 434
PspPI GGNCC 3 cut(s) 92, 503, 596
PspPPI RGGWCCY 1 cut(s) 596
PsuI RGATCY 2 cut(s) 66, 161
RsaI GTAC 2 cut(s) 337, 778
RsaNI GTAC 2 cut(s) 336, 777
RseI CAYNNNNRTG 3 cut(s) 282, 476, 735
SaqAI TTAA 3 cut(s) 27, 483, 820
SatI GCNGC 2 cut(s) 37, 145
Sau3AI GATC 7 cut(s) 60, 66, 161, 394, 490, 572, 810
Sau96I GGNCC 3 cut(s) 92, 503, 596
SchI GAGTC 1 cut(s) 598
SfcI CTRYAG 1 cut(s) 660
SfoI GGCGCC 1 cut(s) 119
SinI GGWCC 2 cut(s) 503, 596
SmiMI CAYNNNNRTG 3 cut(s) 282, 476, 735
SpeI ACTAGT 1 cut(s) 445
Sse9I AATT 7 cut(s) 299, 358, 378, 423, 531, 741, 817
SsiI CCGC 5 cut(s) 87, 100, 112, 147, 642
SspDI GGCGCC 1 cut(s) 117
SspI AATATT 1 cut(s) 701
SspMI CTAG 2 cut(s) 446, 593
StyI CCWWGG 1 cut(s) 95
TaiI ACGT 1 cut(s) 81
TaqI TCGA 3 cut(s) 280, 367, 393
TasI AATT 7 cut(s) 299, 358, 378, 423, 531, 741, 817
TatI WGTACW 2 cut(s) 335, 776
TfiI GAWTC 2 cut(s) 231, 715
Tru1I TTAA 3 cut(s) 27, 483, 820
Tru9I TTAA 3 cut(s) 27, 483, 820
TseI GCWGC 2 cut(s) 36, 144
TspDTI ATGAA 4 cut(s) 300, 401, 477, 753
VpaK11BI GGWCC 2 cut(s) 503, 596
XapI RAATTY 4 cut(s) 299, 378, 423, 531
XceI RCATGY 1 cut(s) 734
XspI CTAG 2 cut(s) 446, 593
Zsp2I ATGCAT 2 cut(s) 688, 736
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.