MD05G1184300.v1.1

Glutathione S-transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000081
Physical Location & Seq
Forward (+)
31259973 .. 31261200
1228 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1184300.v1.1.491

Sequence Viewer

Length: 666 bp
ATGGAAGAAGTGAAGCTGCTAGGTGCATGGCCTAGTCACTTTTGTTACAGAGTCATATGGGCTCTAAAACTAAAGGGTGTCGAGTATGAATATGTCGAAGAAAATCTTACCACCAAGAGTGAACGCCTTCTGCAGCATAACCCAGTTCATAAGAAGATCCCTGTTCTCGTTCATGGCGGAAAACCAATCCCAGAGTCCACTATCATTCTTGAATACATTGAGGAGGTTTGGCCACAGAACCCACTGCTTTCGGATGATCCTTATGAGAGGGCCATGGCTCGATTTTGGATTAAGTTCGAAGAGGACAAGAGTTCAGTTTTTATTGCATATTTTCGATCAATTGGTGAAGAGCAGGAGAAGGCAAAAAAAGAAGCCCAAGAAGCGTTGAAAACAATAGAACATGGACTTGGAGATAAGAAATTTTTTGGAGGGGAGAAAATTGGGTTGGCAGACTTGGCTTTGGGATGGATAGCTGGTTGGCTAGGAGCTATGGAAGAATCAGCTGGTTTAAAGTTGCTAGAACCAAATATGTTTCCTCACTTGCAAACTTGGATACGCAATTTCAAAGATGTGCCTGAGATCAAGGAGAACATACCTCATCATGATGAATTGTTGGCTTATTTCAAACCCCTAAGAGAGAAGTTCATTGCATCATCAAGATCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00480 ko00980 ko00982 ko00983 ko01524 ko05200 ko05204 ko05225 ko05418 map00480 map00980 map00982 map00983 map01524 map05200 map05204 map05225 map05418
Pfam Domains
Protein Families
GST_C GST_N

Protein Analysis

222

Amino Acids

25.6

Weight (kDa)

5.81

Isoelectric Point (pI)

41.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N PF02798 3 - 75 7.1e-19 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 12 - 75 2.4e-14 Glutathione S-transferase, N-terminal domain
GST_N_3 PF13417 12 - 79 3.1e-14 Glutathione S-transferase, N-terminal domain
GST_C PF00043 116 - 188 2.6e-09 Glutathione S-transferase, C-terminal domain
GST_C_2 PF13410 118 - 186 3.9e-07 Glutathione S-transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000081)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G29420
fragaria_vesca FvH4_2g20010 FvH4_2g20020 FvH4_2g20030 FvH4_2g20060 FvH4_2g20070 FvH4_2g20080 FvH4_2g20091 FvH4_2g20110 FvH4_4g05320 FvH4_4g05350 FvH4_4g05360 FvH4_4g12720 FvH4_5g16920 FvH4_5g16920 FvH4_5g16920 FvH4_5g16920 FvH4_5g36020 FvH4_5g36870 FvH4_7g07360 FvH4_7g07390 FvH4_7g09460 FvH4_7g09460 FvH4_7g09460 FvH4_7g09460 FvH4_7g09460
malus_domestica MD00G1136300.v1.1 MD02G1236000.v1.1 MD02G1236100.v1.1 MD05G1184200.v1.1 MD05G1184300.v1.1 MD05G1184400.v1.1 MD05G1184600.v1.1 MD10G1172100.v1.1 MD10G1172200.v1.1 MD10G1172300.v1.1 MD13G1227600.v1.1 MD14G1232100.v1.1 MD14G1232200.v1.1 MD16G1011600.v1.1 MD16G1232800.v1.1 MD16G1232900.v1.1
prunus_persica Prupe.1G039900_v2.0.a1 Prupe.1G054800_v2.0.a1 Prupe.1G054900_v2.0.a1 Prupe.1G055000_v2.0.a1 Prupe.1G055300_v2.0.a1 Prupe.1G055900_v2.0.a1 Prupe.1G056000_v2.0.a1 Prupe.1G056100_v2.0.a1 Prupe.1G526000_v2.0.a1 Prupe.2G101400_v2.0.a1 Prupe.2G101500_v2.0.a1 Prupe.2G101600_v2.0.a1 Prupe.5G228000_v2.0.a1 Prupe.5G228100_v2.0.a1 Prupe.8G210200_v2.0.a1 Prupe.8G210300_v2.0.a1 Prupe.8G210400_v2.0.a1 Prupe.8G210500_v2.0.a1 Prupe.8G210600_v2.0.a1 Prupe.8G210700_v2.0.a1 Prupe.8G210800_v2.0.a1 Prupe.8G210800_v2.0.a1
pyrus_communis pycom02g20280 pycom02g20290 pycom02g20300 pycom05g16920 pycom05g16930 pycom05g16940 pycom07g06270 pycom10g14850 pycom10g14860 pycom10g14870 pycom14g19350 pycom16g19480
rosa_chinensis RchiOBHm_Chr1g0327741 RchiOBHm_Chr1g0339611 RchiOBHm_Chr1g0339631 RchiOBHm_Chr1g0339651 RchiOBHm_Chr1g0339671 RchiOBHm_Chr1g0339691 RchiOBHm_Chr1g0339761 RchiOBHm_Chr1g0339771 RchiOBHm_Chr1g0339781 RchiOBHm_Chr1g0364471 RchiOBHm_Chr1g0364481 RchiOBHm_Chr1g0364501 RchiOBHm_Chr1g0364511 RchiOBHm_Chr4g0396351 RchiOBHm_Chr4g0396391 RchiOBHm_Chr4g0396421 RchiOBHm_Chr4g0396431 RchiOBHm_Chr4g0396441 RchiOBHm_Chr4g0396461 RchiOBHm_Chr4g0396521 RchiOBHm_Chr4g0400161 RchiOBHm_Chr6g0285721 RchiOBHm_Chr6g0285731 RchiOBHm_Chr6g0285741 RchiOBHm_Chr6g0285761 RchiOBHm_Chr6g0285771 RchiOBHm_Chr6g0285781 RchiOBHm_Chr6g0285791 RchiOBHm_Chr6g0285801 RchiOBHm_Chr6g0285821 RchiOBHm_Chr6g0285831 RchiOBHm_Chr7g0179081 RchiOBHm_Chr7g0179091 RchiOBHm_Chr7g0179101 RchiOBHm_Chr7g0215201 RchiOBHm_Chr7g0237741
rosa_laevigata RLG00000000971 RLG00000002403 RLG00000005385 RLG00000005386 RLG00000005387 RLG00000009530 RLG00000009533 RLG00000009534 RLG00000009545 RLG00000009547 RLG00000009548 RLG00000012606 RLG00000012607 RLG00000012608 RLG00000012609 RLG00000012612 RLG00000012613 RLG00000012614 RLG00000015071 RLG00000020454 RLG00000020455 RLG00000027472 RLG00000027473 RLG00000027474 RLG00000027477 RLG00000029240 RLG00000029241
rosa_multiflora Rmu_co8321391.1_g000001 Rmu_co8330237.1_g000001 Rmu_sc0000037.1_g000019 Rmu_sc0000483.1_g000002 Rmu_sc0000511.1_g000018 Rmu_sc0000888.1_g000016 Rmu_sc0000888.1_g000017 Rmu_sc0001356.1_g000009 Rmu_sc0001356.1_g000010 Rmu_sc0001781.1_g000011 Rmu_sc0001781.1_g000020 Rmu_sc0001781.1_g000022 Rmu_sc0001781.1_g000023 Rmu_sc0001781.1_g000043 Rmu_sc0002862.1_g000013 Rmu_sc0003707.1_g000006 Rmu_sc0003707.1_g000007 Rmu_sc0003919.1_g000009 Rmu_sc0004321.1_g000006 Rmu_sc0005500.1_g000001 Rmu_sc0007814.1_g000001 Rmu_sc0007814.1_g000002 Rmu_sc0007814.1_g000010 Rmu_sc0009238.1_g000007 Rmu_sc0009409.1_g000003 Rmu_sc0011138.1_g000004 Rmu_sc0013252.1_g000003 Rmu_sc0017632.1_g000005 Rmu_sc0021325.1_g000002 Rmu_sc0021325.1_g000004 Rmu_sc0021325.1_g000005 Rmu_sc0021325.1_g000008 Rmu_sc0021325.1_g000009 Rmu_sc0028323.1_g000001 Rmu_sc0036111.1_g000001 Rmu_sc0041231.1_g000001 Rmu_ssc0000067.1_g000034 Rmu_ssc0000234.1_g000019 Rmu_ssc0000278.1_g000016
rosa_roxburghii Rroxscaffold_3G00244460 Rroxscaffold_3G00274390 Rroxscaffold_3G00274400 Rroxscaffold_3G00274410 Rroxscaffold_4G00291800 Rroxscaffold_4G00291810 Rroxscaffold_4G00292220 Rroxscaffold_4G00292230 Rroxscaffold_4G00292250 Rroxscaffold_4G00313630 Rroxscaffold_4G00313640 Rroxscaffold_4G00313650 Rroxscaffold_4G00313660 Rroxscaffold_4G00313670 Rroxscaffold_4G00313690 Rroxscaffold_5G00341170 Rroxscaffold_5G00341180 Rroxscaffold_5G00341200 Rroxscaffold_5G00341210 Rroxscaffold_5G00341340 Rroxscaffold_5G00341350 Rroxscaffold_5G00341360 Rroxscaffold_5G00341380 Rroxscaffold_5G00345120 Rroxscaffold_7G00182670 Rroxscaffold_7G00182680 Rroxscaffold_7G00182700 Rroxscaffold_7G00182710 Rroxscaffold_7G00182730 Rroxscaffold_7G00182770 Rroxscaffold_7G00182790 Rroxscaffold_7G00182800 Rroxscaffold_7G00212460
rosa_rugosa Rorug01G0139800.1 Rorug01G0140000.1 Rorug03G0349500 Rorug03G0349500 Rorug03G0349500 Rorug03G0349600 Rorug03G0349600 Rorug04G0023100 Rorug05G0538200 Rorug06G0176800 Rorug06G0177100 Rorug06G0420800 Rorug06G0420900 Rorug06G0421000 Rorug07G0307700
rosa_samantha Rh1AG086600 Rh1AG324800 Rh1DG318900 Rh4AG067600 Rh4AG068400 Rh4AG069200 Rh4BG096900 Rh4CG073400 Rh4CG073900 Rh4CG074500 Rh4DG063500 Rh4DG063700 Rh4DG063800 Rh4DG064000 Rh4DG093500 Rh6AG288300 Rh6AG288400 Rh6AG288500 Rh6AG288600 Rh6AG288700 Rh6AG288800 Rh6BG289900 Rh6BG290000 Rh6BG290100 Rh6BG290400 Rh6BG290800 Rh6BG290900 Rh6CG047000 Rh6CG290900 Rh6CG291000 Rh6CG291100 Rh6CG291300 Rh6CG291400 Rh6CG291500 Rh6CG291600 Rh6CG291700 Rh6CG291800 Rh6DG042500 Rh7AG020300 Rh7AG287600 Rh7BG020000 Rh7CG021200 Rh7CG021400 Rh7CG307400 Rh7DG020900
rosa_wichuraiana Rw0G006240 Rw0G019430 Rw1G013000 Rw1G013020 Rw1G013040 Rw1G013070 Rw1G013090 Rw1G028790 Rw1G028800 Rw1G028810 Rw4G005480 Rw4G005500 Rw4G005520 Rw4G005530 Rw4G005540 Rw4G005560 Rw4G005570 Rw6G004910 Rw6G024770 Rw6G024780 Rw6G024790 Rw6G024800 Rw6G024810 Rw6G024820 Rw6G024830 Rw6G024840 Rw6G024850 Rw6G024860 Rw6G024870 Rw6G024880 Rw6G024890 Rw7G001680 Rw7G001690 Rw7G001700 Rw7G038360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 177
AclWI GGATC 2 cut(s) 151, 251
AcoI YGGCCR 1 cut(s) 230
AcsI RAATTY 1 cut(s) 419
AgsI TTSAA 4 cut(s) 212, 388, 565, 625
AjuI GAANNNNNNNTTGG 4 cut(s) 390, 422, 428, 460
AluBI AGCT 4 cut(s) 16, 473, 488, 503
AluI AGCT 4 cut(s) 16, 473, 488, 503
AlwI GGATC 2 cut(s) 151, 251
AoxI GGCC 3 cut(s) 29, 230, 270
ApeKI GCWGC 2 cut(s) 16, 133
ApoI RAATTY 1 cut(s) 419
Asp700I GAANNNNTTC 1 cut(s) 126
AspS9I GGNCC 1 cut(s) 270
AsuHPI GGTGA 1 cut(s) 356
AsuII TTCGAA 1 cut(s) 297
BalI TGGCCA 1 cut(s) 232
BanII GRGCYC 1 cut(s) 64
BbvI GCAGC 2 cut(s) 3, 145
BccI CCATC 1 cut(s) 459
BciVI GTATCC 1 cut(s) 546
BfaI CTAG 4 cut(s) 20, 33, 482, 518
BfmI CTRYAG 1 cut(s) 131
BfuI GTATCC 1 cut(s) 546
BisI GCNGC 2 cut(s) 17, 134
BlsI GCNGC 2 cut(s) 18, 135
BmgT120I GGNCC 1 cut(s) 270
BmrI ACTGGG 1 cut(s) 137
BmsI GCATC 1 cut(s) 659
BmuI ACTGGG 1 cut(s) 137
Bpu14I TTCGAA 1 cut(s) 297
BsaJI CCNNGG 1 cut(s) 273
Bse1I ACTGG 1 cut(s) 143
Bse3DI GCAATG 1 cut(s) 645
BseDI CCNNGG 1 cut(s) 273
BseGI GGATG 2 cut(s) 259, 470
BseMI GCAATG 1 cut(s) 645
BseMII CTCAG 1 cut(s) 567
BseNI ACTGG 1 cut(s) 143
BseRI GAGGAG 1 cut(s) 236
BseXI GCAGC 2 cut(s) 3, 145
BshFI GGCC 3 cut(s) 31, 232, 272
BsnI GGCC 3 cut(s) 31, 232, 272
Bsp119I TTCGAA 1 cut(s) 297
Bsp1286I GDGCHC 1 cut(s) 64
Bsp143I GATC 5 cut(s) 156, 256, 335, 579, 659
Bsp19I CCATGG 1 cut(s) 273
BspACI CCGC 1 cut(s) 177
BspANI GGCC 3 cut(s) 31, 232, 272
BspCNI CTCAG 1 cut(s) 568
BspHI TCATGA 1 cut(s) 601
BspMAI CTGCAG 1 cut(s) 135
BspPI GGATC 2 cut(s) 151, 251
BspQI GCTCTTC 1 cut(s) 342
BspT104I TTCGAA 1 cut(s) 297
BsrDI GCAATG 1 cut(s) 645
BsrI ACTGG 1 cut(s) 143
BssECI CCNNGG 1 cut(s) 273
BssMI GATC 5 cut(s) 156, 256, 335, 579, 659
BssT1I CCWWGG 1 cut(s) 273
Bst6I CTCTTC 2 cut(s) 294, 342
BstBI TTCGAA 1 cut(s) 297
BstDEI CTNAG 2 cut(s) 576, 632
BstDSI CCRYGG 1 cut(s) 273
BstF5I GGATG 2 cut(s) 259, 470
BstKTI GATC 5 cut(s) 159, 259, 338, 582, 662
BstMBI GATC 5 cut(s) 156, 256, 335, 579, 659
BstMWI GCNNNNNNNGC 2 cut(s) 380, 455
BstSFI CTRYAG 1 cut(s) 131
BstV1I GCAGC 2 cut(s) 3, 145
BstX2I RGATCY 1 cut(s) 156
BstYI RGATCY 1 cut(s) 156
BsuI GTATCC 1 cut(s) 546
BsuRI GGCC 3 cut(s) 31, 232, 272
BtgI CCRYGG 1 cut(s) 273
BtsCI GGATG 2 cut(s) 259, 470
BtsI GCAGTG 1 cut(s) 242
BtsIMutI CAGTG 1 cut(s) 242
CciI TCATGA 1 cut(s) 601
Cfr13I GGNCC 1 cut(s) 270
CviAII CATG 5 cut(s) 27, 173, 274, 401, 602
DdeI CTNAG 2 cut(s) 576, 632
DpnI GATC 5 cut(s) 158, 258, 337, 581, 661
DpnII GATC 5 cut(s) 156, 256, 335, 579, 659
DraI TTTAAA 1 cut(s) 510
EaeI YGGCCR 1 cut(s) 230
Eam1104I CTCTTC 2 cut(s) 294, 342
EarI CTCTTC 2 cut(s) 294, 342
EciI GGCGGA 1 cut(s) 192
Eco130I CCWWGG 1 cut(s) 273
Eco24I GRGCYC 1 cut(s) 64
EcoT14I CCWWGG 1 cut(s) 273
EcoT38I GRGCYC 1 cut(s) 64
ErhI CCWWGG 1 cut(s) 273
FaeI CATG 5 cut(s) 30, 176, 277, 404, 605
FalI AAGNNNNNCTT 2 cut(s) 90, 122
FatI CATG 5 cut(s) 26, 172, 273, 400, 601
FauNDI CATATG 1 cut(s) 56
Fnu4HI GCNGC 2 cut(s) 17, 134
FokI GGATG 2 cut(s) 266, 477
FriOI GRGCYC 1 cut(s) 64
Fsp4HI GCNGC 2 cut(s) 17, 134
FspBI CTAG 4 cut(s) 20, 33, 482, 518
GluI GCNGC 2 cut(s) 17, 134
HaeIII GGCC 3 cut(s) 31, 232, 272
Hin1II CATG 5 cut(s) 30, 176, 277, 404, 605
HinfI GANTC 3 cut(s) 51, 194, 497
HphI GGTGA 1 cut(s) 356
Hpy166II GTNNAC 2 cut(s) 122, 198
Hpy188I TCNGA 1 cut(s) 253
Hpy188III TCNNGA 3 cut(s) 209, 602, 657
Hpy8I GTNNAC 2 cut(s) 122, 198
HpyAV CCTTC 2 cut(s) 137, 352
HpyCH4V TGCA 5 cut(s) 26, 133, 326, 544, 650
HpyF10VI GCNNNNNNNGC 2 cut(s) 380, 455
HpyF3I CTNAG 2 cut(s) 576, 632
Hsp92II CATG 5 cut(s) 30, 176, 277, 404, 605
Kzo9I GATC 5 cut(s) 156, 256, 335, 579, 659
LguI GCTCTTC 1 cut(s) 342
LmnI GCTCC 1 cut(s) 485
LpnPI CCDG 7 cut(s) 156, 174, 204, 338, 459, 489, 588
Lsp1109I GCAGC 2 cut(s) 3, 145
LweI GCATC 1 cut(s) 659
MaeI CTAG 4 cut(s) 20, 33, 482, 518
MaeIII GTNAC 2 cut(s) 35, 44
MalI GATC 5 cut(s) 158, 258, 337, 581, 661
MboI GATC 5 cut(s) 156, 256, 335, 579, 659
MboII GAAGA 6 cut(s) 17, 110, 166, 311, 359, 506
MfeI CAATTG 1 cut(s) 339
MflI RGATCY 1 cut(s) 156
MhlI GDGCHC 1 cut(s) 64
MlsI TGGCCA 1 cut(s) 232
MluCI AATT 5 cut(s) 339, 419, 438, 559, 608
MluNI TGGCCA 1 cut(s) 232
MlyI GAGTC 2 cut(s) 60, 203
MnlI CCTC 7 cut(s) 214, 217, 261, 295, 422, 546, 606
Mox20I TGGCCA 1 cut(s) 232
MroXI GAANNNNTTC 1 cut(s) 126
MscI TGGCCA 1 cut(s) 232
MseI TTAA 2 cut(s) 291, 509
MslI CAYNNNNRTG 1 cut(s) 603
Msp20I TGGCCA 1 cut(s) 232
MspA1I CMGCKG 1 cut(s) 503
MunI CAATTG 1 cut(s) 339
MwoI GCNNNNNNNGC 2 cut(s) 380, 455
NcoI CCATGG 1 cut(s) 273
NdeI CATATG 1 cut(s) 56
NdeII GATC 5 cut(s) 156, 256, 335, 579, 659
NlaIII CATG 5 cut(s) 30, 176, 277, 404, 605
NmuCI GTSAC 1 cut(s) 35
NspV TTCGAA 1 cut(s) 297
PagI TCATGA 1 cut(s) 601
PciSI GCTCTTC 1 cut(s) 342
PdmI GAANNNNTTC 1 cut(s) 126
PfeI GAWTC 1 cut(s) 497
PkrI GCNGC 2 cut(s) 18, 135
PleI GAGTC 2 cut(s) 59, 202
PpsI GAGTC 2 cut(s) 59, 202
PspPI GGNCC 1 cut(s) 270
PstI CTGCAG 1 cut(s) 135
PsuI RGATCY 1 cut(s) 156
PvuII CAGCTG 1 cut(s) 503
RseI CAYNNNNRTG 1 cut(s) 603
SapI GCTCTTC 1 cut(s) 342
SaqAI TTAA 2 cut(s) 291, 509
SatI GCNGC 2 cut(s) 17, 134
Sau3AI GATC 5 cut(s) 156, 256, 335, 579, 659
Sau96I GGNCC 1 cut(s) 270
SchI GAGTC 2 cut(s) 60, 203
SduI GDGCHC 1 cut(s) 64
SetI ASST 7 cut(s) 18, 25, 228, 475, 490, 505, 598
SfaNI GCATC 1 cut(s) 659
SfcI CTRYAG 1 cut(s) 131
SfuI TTCGAA 1 cut(s) 297
SmiMI CAYNNNNRTG 1 cut(s) 603
Sse9I AATT 5 cut(s) 339, 419, 438, 559, 608
SsiI CCGC 1 cut(s) 177
SspMI CTAG 4 cut(s) 20, 33, 482, 518
StyI CCWWGG 1 cut(s) 273
TaqI TCGA 5 cut(s) 81, 96, 280, 297, 334
TasI AATT 5 cut(s) 339, 419, 438, 559, 608
TfiI GAWTC 1 cut(s) 497
Tru1I TTAA 2 cut(s) 291, 509
Tru9I TTAA 2 cut(s) 291, 509
TscAI CASTG 1 cut(s) 249
TseFI GTSAC 1 cut(s) 35
TseI GCWGC 2 cut(s) 16, 133
Tsp45I GTSAC 1 cut(s) 35
TspDTI ATGAA 5 cut(s) 102, 137, 161, 621, 634
TspRI CASTG 1 cut(s) 249
XapI RAATTY 1 cut(s) 419
XmnI GAANNNNTTC 1 cut(s) 126
XspI CTAG 4 cut(s) 20, 33, 482, 518
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.