Belongs to the GST superfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 346 |
| Acc36I | ACCTGC | 1 cut(s) | 346 |
| AciI | CCGC | 1 cut(s) | 177 |
| AcoI | YGGCCR | 1 cut(s) | 230 |
| AcsI | RAATTY | 2 cut(s) | 434, 459 |
| AcuI | CTGAAG | 1 cut(s) | 519 |
| AfaI | GTAC | 1 cut(s) | 418 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 282, 640 |
| AgsI | TTSAA | 5 cut(s) | 67, 367, 509, 536, 580 |
| AjiI | CACGTC | 1 cut(s) | 514 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 362, 394 |
| AluBI | AGCT | 4 cut(s) | 16, 127, 378, 529 |
| AluI | AGCT | 4 cut(s) | 16, 127, 378, 529 |
| Alw21I | GWGCWC | 2 cut(s) | 129, 168 |
| Alw26I | GTCTC | 2 cut(s) | 337, 441 |
| AoxI | GGCC | 2 cut(s) | 182, 230 |
| ApeKI | GCWGC | 1 cut(s) | 410 |
| ApoI | RAATTY | 2 cut(s) | 434, 459 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 105, 606 |
| AspS9I | GGNCC | 2 cut(s) | 314, 631 |
| AsuC2I | CCSGG | 1 cut(s) | 161 |
| AsuHPI | GGTGA | 4 cut(s) | 22, 64, 370, 535 |
| AvaII | GGWCC | 2 cut(s) | 314, 631 |
| AxyI | CCTNAGG | 1 cut(s) | 475 |
| BalI | TGGCCA | 1 cut(s) | 232 |
| BanII | GRGCYC | 1 cut(s) | 129 |
| BbsI | GAAGAC | 1 cut(s) | 516 |
| Bbv12I | GWGCWC | 2 cut(s) | 129, 168 |
| BbvCI | CCTCAGC | 1 cut(s) | 6 |
| BbvI | GCAGC | 1 cut(s) | 397 |
| BccI | CCATC | 1 cut(s) | 322 |
| BceAI | ACGGC | 1 cut(s) | 260 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 389, 423 |
| BciVI | GTATCC | 1 cut(s) | 473 |
| BclI | TGATCA | 1 cut(s) | 201 |
| BcnI | CCSGG | 1 cut(s) | 161 |
| BcoDI | GTCTC | 2 cut(s) | 337, 441 |
| BfuAI | ACCTGC | 1 cut(s) | 346 |
| BfuI | GTATCC | 1 cut(s) | 473 |
| BglII | AGATCT | 1 cut(s) | 103 |
| BisI | GCNGC | 1 cut(s) | 411 |
| BlsI | GCNGC | 1 cut(s) | 412 |
| Bme1390I | CCNGG | 1 cut(s) | 161 |
| Bme18I | GGWCC | 2 cut(s) | 314, 631 |
| BmgBI | CACGTC | 1 cut(s) | 514 |
| BmgT120I | GGNCC | 2 cut(s) | 314, 631 |
| BmiI | GGNNCC | 1 cut(s) | 315 |
| BmrFI | CCNGG | 1 cut(s) | 161 |
| BmsI | GCATC | 1 cut(s) | 87 |
| BoxI | GACNNNNGTC | 1 cut(s) | 500 |
| BpiI | GAAGAC | 1 cut(s) | 516 |
| Bpu10I | CCTNAGC | 2 cut(s) | 6, 123 |
| BpuMI | CCSGG | 1 cut(s) | 161 |
| BsaI | GGTCTC | 1 cut(s) | 337 |
| BsaJI | CCNNGG | 1 cut(s) | 276 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 282, 640 |
| Bse1I | ACTGG | 1 cut(s) | 349 |
| Bse21I | CCTNAGG | 1 cut(s) | 475 |
| Bse3DI | GCAATG | 1 cut(s) | 483 |
| BseDI | CCNNGG | 1 cut(s) | 276 |
| BseGI | GGATG | 2 cut(s) | 78, 333 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 282, 640 |
| BseMI | GCAATG | 1 cut(s) | 483 |
| BseMII | CTCAG | 2 cut(s) | 114, 584 |
| BseNI | ACTGG | 1 cut(s) | 349 |
| BseRI | GAGGAG | 2 cut(s) | 119, 459 |
| BseXI | GCAGC | 1 cut(s) | 397 |
| BseYI | CCCAGC | 1 cut(s) | 518 |
| BshFI | GGCC | 2 cut(s) | 184, 232 |
| BsiHKAI | GWGCWC | 2 cut(s) | 129, 168 |
| BsiSI | CCGG | 1 cut(s) | 161 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 282, 640 |
| BsmAI | GTCTC | 2 cut(s) | 337, 441 |
| BsmI | GAATGC | 1 cut(s) | 545 |
| BsnI | GGCC | 2 cut(s) | 184, 232 |
| Bso31I | GGTCTC | 1 cut(s) | 337 |
| Bsp1286I | GDGCHC | 2 cut(s) | 129, 168 |
| Bsp143I | GATC | 3 cut(s) | 103, 201, 613 |
| BspACI | CCGC | 1 cut(s) | 177 |
| BspANI | GGCC | 2 cut(s) | 184, 232 |
| BspCNI | CTCAG | 2 cut(s) | 115, 583 |
| BspLI | GGNNCC | 1 cut(s) | 315 |
| BspMI | ACCTGC | 1 cut(s) | 346 |
| BspTNI | GGTCTC | 1 cut(s) | 337 |
| BsrDI | GCAATG | 1 cut(s) | 483 |
| BsrI | ACTGG | 1 cut(s) | 349 |
| BssECI | CCNNGG | 1 cut(s) | 276 |
| BssMI | GATC | 3 cut(s) | 103, 201, 613 |
| BstC8I | GCNNGC | 2 cut(s) | 561, 591 |
| BstDEI | CTNAG | 4 cut(s) | 6, 123, 475, 570 |
| BstDSI | CCRYGG | 1 cut(s) | 276 |
| BstF5I | GGATG | 2 cut(s) | 78, 333 |
| BstKTI | GATC | 3 cut(s) | 106, 204, 616 |
| BstMAI | GTCTC | 2 cut(s) | 337, 441 |
| BstMBI | GATC | 3 cut(s) | 103, 201, 613 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 238 |
| BstNSI | RCATGY | 1 cut(s) | 563 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 500 |
| BstSCI | CCNGG | 1 cut(s) | 159 |
| BstV1I | GCAGC | 1 cut(s) | 397 |
| BstV2I | GAAGAC | 1 cut(s) | 516 |
| BstX2I | RGATCY | 1 cut(s) | 103 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 344, 413 |
| BstYI | RGATCY | 1 cut(s) | 103 |
| Bsu36I | CCTNAGG | 1 cut(s) | 475 |
| BsuI | GTATCC | 1 cut(s) | 473 |
| BsuRI | GGCC | 2 cut(s) | 184, 232 |
| BtgI | CCRYGG | 1 cut(s) | 276 |
| BtrI | CACGTC | 1 cut(s) | 514 |
| BtsCI | GGATG | 2 cut(s) | 78, 333 |
| BtsIMutI | CAGTG | 2 cut(s) | 255, 342 |
| BveI | ACCTGC | 1 cut(s) | 346 |
| Cac8I | GCNNGC | 2 cut(s) | 561, 591 |
| Cfr13I | GGNCC | 2 cut(s) | 314, 631 |
| Csp6I | GTAC | 1 cut(s) | 417 |
| CviAII | CATG | 7 cut(s) | 20, 173, 199, 228, 407, 542, 560 |
| CviQI | GTAC | 1 cut(s) | 417 |
| DdeI | CTNAG | 4 cut(s) | 6, 123, 475, 570 |
| DpnI | GATC | 3 cut(s) | 105, 203, 615 |
| DpnII | GATC | 3 cut(s) | 103, 201, 613 |
| EaeI | YGGCCR | 1 cut(s) | 230 |
| Ecl136II | GAGCTC | 1 cut(s) | 127 |
| Eco24I | GRGCYC | 1 cut(s) | 129 |
| Eco31I | GGTCTC | 1 cut(s) | 337 |
| Eco47I | GGWCC | 2 cut(s) | 314, 631 |
| Eco53kI | GAGCTC | 1 cut(s) | 127 |
| Eco57I | CTGAAG | 1 cut(s) | 519 |
| Eco81I | CCTNAGG | 1 cut(s) | 475 |
| EcoICRI | GAGCTC | 1 cut(s) | 127 |
| EcoO109I | RGGNCCY | 1 cut(s) | 314 |
| EcoT22I | ATGCAT | 1 cut(s) | 547 |
| EcoT38I | GRGCYC | 1 cut(s) | 129 |
| FaeI | CATG | 7 cut(s) | 23, 176, 202, 231, 410, 545, 563 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 90, 122, 230, 262, 591, 623 |
| FatI | CATG | 7 cut(s) | 19, 172, 198, 227, 406, 541, 559 |
| FauI | CCCGC | 1 cut(s) | 170 |
| FauNDI | CATATG | 1 cut(s) | 85 |
| FbaI | TGATCA | 1 cut(s) | 201 |
| Fnu4HI | GCNGC | 1 cut(s) | 411 |
| FokI | GGATG | 2 cut(s) | 65, 340 |
| FriOI | GRGCYC | 1 cut(s) | 129 |
| Fsp4HI | GCNGC | 1 cut(s) | 411 |
| GluI | GCNGC | 1 cut(s) | 411 |
| GsaI | CCCAGC | 1 cut(s) | 522 |
| HaeIII | GGCC | 2 cut(s) | 184, 232 |
| HapII | CCGG | 1 cut(s) | 161 |
| Hin1II | CATG | 7 cut(s) | 23, 176, 202, 231, 410, 545, 563 |
| HinfI | GANTC | 2 cut(s) | 194, 568 |
| HpaII | CCGG | 1 cut(s) | 161 |
| HphI | GGTGA | 4 cut(s) | 22, 64, 370, 535 |
| Hpy166II | GTNNAC | 2 cut(s) | 148, 634 |
| Hpy188I | TCNGA | 1 cut(s) | 573 |
| Hpy188III | TCNNGA | 4 cut(s) | 122, 367, 611, 617 |
| Hpy8I | GTNNAC | 2 cut(s) | 148, 634 |
| Hpy99I | CGWCG | 1 cut(s) | 353 |
| HpyAV | CCTTC | 2 cut(s) | 256, 617 |
| HpyCH4IV | ACGT | 1 cut(s) | 513 |
| HpyCH4V | TGCA | 5 cut(s) | 48, 320, 545, 559, 593 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 238 |
| HpyF3I | CTNAG | 4 cut(s) | 6, 123, 475, 570 |
| HpySE526I | ACGT | 1 cut(s) | 513 |
| Hsp92II | CATG | 7 cut(s) | 23, 176, 202, 231, 410, 545, 563 |
| Ksp22I | TGATCA | 1 cut(s) | 201 |
| Kzo9I | GATC | 3 cut(s) | 103, 201, 613 |
| LmnI | GCTCC | 2 cut(s) | 30, 132 |
| Lsp1109I | GCAGC | 1 cut(s) | 397 |
| LweI | GCATC | 1 cut(s) | 87 |
| MaeII | ACGT | 1 cut(s) | 513 |
| MalI | GATC | 3 cut(s) | 105, 203, 615 |
| MboI | GATC | 3 cut(s) | 103, 201, 613 |
| MboII | GAAGA | 6 cut(s) | 98, 110, 113, 233, 317, 521 |
| MflI | RGATCY | 1 cut(s) | 103 |
| MhlI | GDGCHC | 2 cut(s) | 129, 168 |
| MlsI | TGGCCA | 1 cut(s) | 232 |
| MluCI | AATT | 3 cut(s) | 321, 434, 459 |
| MluNI | TGGCCA | 1 cut(s) | 232 |
| MlyI | GAGTC | 2 cut(s) | 203, 562 |
| MnlI | CCTC | 4 cut(s) | 140, 174, 376, 437 |
| Mox20I | TGGCCA | 1 cut(s) | 232 |
| Mph1103I | ATGCAT | 1 cut(s) | 547 |
| MroXI | GAANNNNTTC | 2 cut(s) | 105, 606 |
| MscI | TGGCCA | 1 cut(s) | 232 |
| MseI | TTAA | 3 cut(s) | 71, 294, 639 |
| Msp20I | TGGCCA | 1 cut(s) | 232 |
| MspI | CCGG | 1 cut(s) | 161 |
| MspR9I | CCNGG | 1 cut(s) | 161 |
| Mva1269I | GAATGC | 1 cut(s) | 545 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 238 |
| NciI | CCSGG | 1 cut(s) | 161 |
| NdeI | CATATG | 1 cut(s) | 85 |
| NdeII | GATC | 3 cut(s) | 103, 201, 613 |
| NlaIII | CATG | 7 cut(s) | 23, 176, 202, 231, 410, 545, 563 |
| NlaIV | GGNNCC | 1 cut(s) | 315 |
| NsiI | ATGCAT | 1 cut(s) | 547 |
| NspI | RCATGY | 1 cut(s) | 563 |
| PaeI | GCATGC | 1 cut(s) | 563 |
| PaqCI | CACCTGC | 1 cut(s) | 346 |
| PctI | GAATGC | 1 cut(s) | 545 |
| PdmI | GAANNNNTTC | 2 cut(s) | 105, 606 |
| PkrI | GCNGC | 1 cut(s) | 412 |
| PleI | GAGTC | 2 cut(s) | 202, 562 |
| PpsI | GAGTC | 2 cut(s) | 202, 562 |
| PpuMI | RGGWCCY | 1 cut(s) | 314 |
| PshAI | GACNNNNGTC | 1 cut(s) | 500 |
| Psp124BI | GAGCTC | 1 cut(s) | 129 |
| Psp5II | RGGWCCY | 1 cut(s) | 314 |
| PspFI | CCCAGC | 1 cut(s) | 518 |
| PspN4I | GGNNCC | 1 cut(s) | 315 |
| PspPI | GGNCC | 2 cut(s) | 314, 631 |
| PspPPI | RGGWCCY | 1 cut(s) | 314 |
| PsuI | RGATCY | 1 cut(s) | 103 |
| RsaI | GTAC | 1 cut(s) | 418 |
| RsaNI | GTAC | 1 cut(s) | 417 |
| SacI | GAGCTC | 1 cut(s) | 129 |
| SaqAI | TTAA | 3 cut(s) | 71, 294, 639 |
| SatI | GCNGC | 1 cut(s) | 411 |
| Sau3AI | GATC | 3 cut(s) | 103, 201, 613 |
| Sau96I | GGNCC | 2 cut(s) | 314, 631 |
| SchI | GAGTC | 2 cut(s) | 203, 562 |
| ScrFI | CCNGG | 1 cut(s) | 161 |
| SduI | GDGCHC | 2 cut(s) | 129, 168 |
| SetI | ASST | 9 cut(s) | 12, 18, 129, 360, 380, 387, 516, 531, 609 |
| SfaNI | GCATC | 1 cut(s) | 87 |
| SinI | GGWCC | 2 cut(s) | 314, 631 |
| SphI | GCATGC | 1 cut(s) | 563 |
| Sse9I | AATT | 3 cut(s) | 321, 434, 459 |
| SsiI | CCGC | 1 cut(s) | 177 |
| SstI | GAGCTC | 1 cut(s) | 129 |
| StyD4I | CCNGG | 1 cut(s) | 159 |
| TaiI | ACGT | 1 cut(s) | 516 |
| TaqI | TCGA | 3 cut(s) | 210, 303, 399 |
| TasI | AATT | 3 cut(s) | 321, 434, 459 |
| Tru1I | TTAA | 3 cut(s) | 71, 294, 639 |
| Tru9I | TTAA | 3 cut(s) | 71, 294, 639 |
| TscAI | CASTG | 2 cut(s) | 262, 349 |
| TseI | GCWGC | 1 cut(s) | 410 |
| TspDTI | ATGAA | 1 cut(s) | 161 |
| TspRI | CASTG | 2 cut(s) | 262, 349 |
| VpaK11BI | GGWCC | 2 cut(s) | 314, 631 |
| XapI | RAATTY | 2 cut(s) | 434, 459 |
| XceI | RCATGY | 1 cut(s) | 563 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 641 |
| XmnI | GAANNNNTTC | 2 cut(s) | 105, 606 |
| Zsp2I | ATGCAT | 1 cut(s) | 547 |