Belongs to the GST superfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 332 |
| AclWI | GGATC | 2 cut(s) | 199, 251 |
| AcsI | RAATTY | 1 cut(s) | 293 |
| AcuI | CTGAAG | 2 cut(s) | 4, 86 |
| AfeI | AGCGCT | 1 cut(s) | 37 |
| AgsI | TTSAA | 5 cut(s) | 101, 134, 212, 221, 421 |
| AluBI | AGCT | 6 cut(s) | 16, 70, 330, 376, 397, 475 |
| AluI | AGCT | 6 cut(s) | 16, 70, 330, 376, 397, 475 |
| Alw21I | GWGCWC | 1 cut(s) | 174 |
| Alw44I | GTGCAC | 1 cut(s) | 170 |
| AlwI | GGATC | 2 cut(s) | 199, 251 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 191 |
| Aor51HI | AGCGCT | 1 cut(s) | 37 |
| ApaLI | GTGCAC | 1 cut(s) | 170 |
| ApeKI | GCWGC | 1 cut(s) | 397 |
| ApoI | RAATTY | 1 cut(s) | 293 |
| AspLEI | GCGC | 1 cut(s) | 38 |
| AsuHPI | GGTGA | 1 cut(s) | 311 |
| BaeGI | GKGCMC | 2 cut(s) | 64, 174 |
| BbsI | GAAGAC | 1 cut(s) | 392 |
| Bbv12I | GWGCWC | 1 cut(s) | 174 |
| BbvI | GCAGC | 1 cut(s) | 384 |
| BccI | CCATC | 2 cut(s) | 101, 374 |
| BceAI | ACGGC | 1 cut(s) | 257 |
| BclI | TGATCA | 1 cut(s) | 408 |
| BfaI | CTAG | 1 cut(s) | 377 |
| BfmI | CTRYAG | 1 cut(s) | 398 |
| BfoI | RGCGCY | 1 cut(s) | 39 |
| BglII | AGATCT | 1 cut(s) | 103 |
| BisI | GCNGC | 1 cut(s) | 398 |
| BlpI | GCTNAGC | 1 cut(s) | 476 |
| BlsI | GCNGC | 1 cut(s) | 399 |
| BmiI | GGNNCC | 1 cut(s) | 159 |
| BpiI | GAAGAC | 1 cut(s) | 392 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 476 |
| BsaBI | GATNNNNATC | 1 cut(s) | 407 |
| Bse1I | ACTGG | 1 cut(s) | 161 |
| Bse8I | GATNNNNATC | 1 cut(s) | 407 |
| BseJI | GATNNNNATC | 1 cut(s) | 407 |
| BseMII | CTCAG | 1 cut(s) | 467 |
| BseNI | ACTGG | 1 cut(s) | 161 |
| BseSI | GKGCMC | 2 cut(s) | 64, 174 |
| BseXI | GCAGC | 1 cut(s) | 384 |
| BsiHKAI | GWGCWC | 1 cut(s) | 174 |
| BsmI | GAATGC | 1 cut(s) | 480 |
| Bsp1286I | GDGCHC | 2 cut(s) | 64, 174 |
| Bsp143I | GATC | 6 cut(s) | 103, 204, 256, 408, 448, 469 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 476 |
| BspCNI | CTCAG | 1 cut(s) | 468 |
| BspLI | GGNNCC | 1 cut(s) | 159 |
| BspMAI | CTGCAG | 1 cut(s) | 402 |
| BspPI | GGATC | 2 cut(s) | 199, 251 |
| BspQI | GCTCTTC | 1 cut(s) | 23 |
| BsrI | ACTGG | 1 cut(s) | 161 |
| BssMI | GATC | 6 cut(s) | 103, 204, 256, 408, 448, 469 |
| Bst4CI | ACNGT | 2 cut(s) | 370, 391 |
| Bst6I | CTCTTC | 1 cut(s) | 23 |
| BstC8I | GCNNGC | 1 cut(s) | 280 |
| BstDEI | CTNAG | 1 cut(s) | 476 |
| BstH2I | RGCGCY | 1 cut(s) | 39 |
| BstHHI | GCGC | 1 cut(s) | 38 |
| BstKTI | GATC | 6 cut(s) | 106, 207, 259, 411, 451, 472 |
| BstMBI | GATC | 6 cut(s) | 103, 204, 256, 408, 448, 469 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 336 |
| BstSFI | CTRYAG | 1 cut(s) | 398 |
| BstSLI | GKGCMC | 2 cut(s) | 64, 174 |
| BstV1I | GCAGC | 1 cut(s) | 384 |
| BstV2I | GAAGAC | 1 cut(s) | 392 |
| BstX2I | RGATCY | 2 cut(s) | 103, 256 |
| BstYI | RGATCY | 2 cut(s) | 103, 256 |
| BtsIMutI | CAGTG | 3 cut(s) | 62, 168, 366 |
| Cac8I | GCNNGC | 1 cut(s) | 280 |
| CaiI | CAGNNNCTG | 1 cut(s) | 191 |
| CfoI | GCGC | 1 cut(s) | 38 |
| CviAII | CATG | 2 cut(s) | 27, 228 |
| CviJI | RGCY | 9 cut(s) | 16, 70, 120, 272, 330, 376, 397, 429, 475 |
| CviKI_1 | RGCY | 9 cut(s) | 16, 70, 120, 272, 330, 376, 397, 429, 475 |
| DdeI | CTNAG | 1 cut(s) | 476 |
| DpnI | GATC | 6 cut(s) | 105, 206, 258, 410, 450, 471 |
| DpnII | GATC | 6 cut(s) | 103, 204, 256, 408, 448, 469 |
| DraI | TTTAAA | 1 cut(s) | 358 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 332 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 332 |
| Eam1104I | CTCTTC | 1 cut(s) | 23 |
| EarI | CTCTTC | 1 cut(s) | 23 |
| Eco47III | AGCGCT | 1 cut(s) | 37 |
| Eco57I | CTGAAG | 2 cut(s) | 4, 86 |
| FaeI | CATG | 2 cut(s) | 30, 231 |
| FaiI | YATR | 7 cut(s) | 28, 87, 150, 229, 321, 333, 464 |
| FatI | CATG | 2 cut(s) | 26, 227 |
| FbaI | TGATCA | 1 cut(s) | 408 |
| Fnu4HI | GCNGC | 1 cut(s) | 398 |
| Fsp4HI | GCNGC | 1 cut(s) | 398 |
| FspBI | CTAG | 1 cut(s) | 377 |
| GlaI | GCGC | 1 cut(s) | 37 |
| GluI | GCNGC | 1 cut(s) | 398 |
| HaeII | RGCGCY | 1 cut(s) | 39 |
| HhaI | GCGC | 1 cut(s) | 38 |
| Hin1II | CATG | 2 cut(s) | 30, 231 |
| Hin6I | GCGC | 1 cut(s) | 36 |
| HinP1I | GCGC | 1 cut(s) | 36 |
| HinfI | GANTC | 2 cut(s) | 194, 314 |
| HphI | GGTGA | 1 cut(s) | 311 |
| Hpy166II | GTNNAC | 2 cut(s) | 172, 325 |
| Hpy188I | TCNGA | 2 cut(s) | 23, 253 |
| Hpy188III | TCNNGA | 2 cut(s) | 263, 452 |
| Hpy8I | GTNNAC | 2 cut(s) | 172, 325 |
| HpyAV | CCTTC | 1 cut(s) | 148 |
| HpyCH4III | ACNGT | 2 cut(s) | 370, 391 |
| HpyCH4V | TGCA | 3 cut(s) | 172, 278, 400 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 336 |
| HpyF3I | CTNAG | 1 cut(s) | 476 |
| Hsp92II | CATG | 2 cut(s) | 30, 231 |
| HspAI | GCGC | 1 cut(s) | 36 |
| Ksp22I | TGATCA | 1 cut(s) | 408 |
| Kzo9I | GATC | 6 cut(s) | 103, 204, 256, 408, 448, 469 |
| LguI | GCTCTTC | 1 cut(s) | 23 |
| LmnI | GCTCC | 1 cut(s) | 373 |
| LpnPI | CCDG | 4 cut(s) | 43, 174, 397, 470 |
| Lsp1109I | GCAGC | 1 cut(s) | 384 |
| MaeI | CTAG | 1 cut(s) | 377 |
| MalI | GATC | 6 cut(s) | 105, 206, 258, 410, 450, 471 |
| MboI | GATC | 6 cut(s) | 103, 204, 256, 408, 448, 469 |
| MboII | GAAGA | 6 cut(s) | 10, 113, 122, 233, 266, 397 |
| MflI | RGATCY | 2 cut(s) | 103, 256 |
| MhlI | GDGCHC | 2 cut(s) | 64, 174 |
| MluCI | AATT | 3 cut(s) | 293, 416, 443 |
| MnlI | CCTC | 1 cut(s) | 270 |
| MseI | TTAA | 1 cut(s) | 357 |
| MspA1I | CMGCKG | 1 cut(s) | 475 |
| Mva1269I | GAATGC | 1 cut(s) | 480 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 336 |
| NdeII | GATC | 6 cut(s) | 103, 204, 256, 408, 448, 469 |
| NlaIII | CATG | 2 cut(s) | 30, 231 |
| NlaIV | GGNNCC | 1 cut(s) | 159 |
| PciSI | GCTCTTC | 1 cut(s) | 23 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 270 |
| PctI | GAATGC | 1 cut(s) | 480 |
| PfeI | GAWTC | 2 cut(s) | 194, 314 |
| PkrI | GCNGC | 1 cut(s) | 399 |
| PspN4I | GGNNCC | 1 cut(s) | 159 |
| PstI | CTGCAG | 1 cut(s) | 402 |
| PstNI | CAGNNNCTG | 1 cut(s) | 191 |
| PsuI | RGATCY | 2 cut(s) | 103, 256 |
| PvuII | CAGCTG | 1 cut(s) | 475 |
| SapI | GCTCTTC | 1 cut(s) | 23 |
| SaqAI | TTAA | 1 cut(s) | 357 |
| SatI | GCNGC | 1 cut(s) | 398 |
| Sau3AI | GATC | 6 cut(s) | 103, 204, 256, 408, 448, 469 |
| SduI | GDGCHC | 2 cut(s) | 64, 174 |
| SetI | ASST | 7 cut(s) | 18, 72, 159, 332, 378, 399, 477 |
| SfcI | CTRYAG | 1 cut(s) | 398 |
| Sse9I | AATT | 3 cut(s) | 293, 416, 443 |
| SspMI | CTAG | 1 cut(s) | 377 |
| TaaI | ACNGT | 2 cut(s) | 370, 391 |
| TasI | AATT | 3 cut(s) | 293, 416, 443 |
| TfiI | GAWTC | 2 cut(s) | 194, 314 |
| Tru1I | TTAA | 1 cut(s) | 357 |
| Tru9I | TTAA | 1 cut(s) | 357 |
| TscAI | CASTG | 3 cut(s) | 69, 168, 373 |
| TseI | GCWGC | 1 cut(s) | 397 |
| TspDTI | ATGAA | 3 cut(s) | 137, 318, 421 |
| TspRI | CASTG | 3 cut(s) | 69, 168, 373 |
| VneI | GTGCAC | 1 cut(s) | 170 |
| XapI | RAATTY | 1 cut(s) | 293 |
| XspI | CTAG | 1 cut(s) | 377 |