Belongs to the GST superfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 617 |
| Acc16I | TGCGCA | 1 cut(s) | 477 |
| AciI | CCGC | 1 cut(s) | 273 |
| AclWI | GGATC | 2 cut(s) | 251, 654 |
| AcoI | YGGCCR | 1 cut(s) | 599 |
| AcsI | RAATTY | 3 cut(s) | 293, 422, 626 |
| AcuI | CTGAAG | 2 cut(s) | 116, 273 |
| AfaI | GTAC | 1 cut(s) | 395 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 279, 345 |
| AgsI | TTSAA | 5 cut(s) | 111, 212, 391, 500, 658 |
| AluBI | AGCT | 4 cut(s) | 16, 365, 409, 442 |
| AluI | AGCT | 4 cut(s) | 16, 365, 409, 442 |
| Alw21I | GWGCWC | 1 cut(s) | 174 |
| Alw26I | GTCTC | 1 cut(s) | 603 |
| Alw44I | GTGCAC | 1 cut(s) | 170 |
| AlwI | GGATC | 2 cut(s) | 251, 654 |
| AoxI | GGCC | 1 cut(s) | 599 |
| ApaLI | GTGCAC | 1 cut(s) | 170 |
| ApeKI | GCWGC | 2 cut(s) | 247, 275 |
| ApoI | RAATTY | 3 cut(s) | 293, 422, 626 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 537 |
| AspLEI | GCGC | 1 cut(s) | 478 |
| AsuHPI | GGTGA | 2 cut(s) | 311, 641 |
| BaeGI | GKGCMC | 2 cut(s) | 64, 174 |
| BalI | TGGCCA | 1 cut(s) | 601 |
| BbsI | GAAGAC | 1 cut(s) | 507 |
| Bbv12I | GWGCWC | 1 cut(s) | 174 |
| BbvI | GCAGC | 2 cut(s) | 234, 262 |
| BccI | CCATC | 4 cut(s) | 325, 338, 462, 610 |
| BcoDI | GTCTC | 1 cut(s) | 603 |
| BfaI | CTAG | 1 cut(s) | 606 |
| BglII | AGATCT | 1 cut(s) | 103 |
| BisI | GCNGC | 3 cut(s) | 248, 273, 276 |
| BlsI | GCNGC | 3 cut(s) | 249, 274, 277 |
| BmcAI | AGTACT | 1 cut(s) | 395 |
| BmiI | GGNNCC | 1 cut(s) | 159 |
| BmsI | GCATC | 1 cut(s) | 565 |
| BpiI | GAAGAC | 1 cut(s) | 507 |
| BpuEI | CTTGAG | 1 cut(s) | 284 |
| BsaJI | CCNNGG | 1 cut(s) | 378 |
| BsaWI | WCCGGW | 1 cut(s) | 509 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 279, 345 |
| Bse1I | ACTGG | 3 cut(s) | 161, 339, 602 |
| BseDI | CCNNGG | 1 cut(s) | 378 |
| BseGI | GGATG | 1 cut(s) | 473 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 279, 345 |
| BseNI | ACTGG | 3 cut(s) | 161, 339, 602 |
| BseSI | GKGCMC | 2 cut(s) | 64, 174 |
| BseXI | GCAGC | 2 cut(s) | 234, 262 |
| BsgI | GTGCAG | 1 cut(s) | 261 |
| BshFI | GGCC | 1 cut(s) | 601 |
| BsiHKAI | GWGCWC | 1 cut(s) | 174 |
| BsiSI | CCGG | 1 cut(s) | 510 |
| BslFI | GGGAC | 1 cut(s) | 65 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 279, 345 |
| BsmAI | GTCTC | 1 cut(s) | 603 |
| BsmFI | GGGAC | 1 cut(s) | 65 |
| BsnI | GGCC | 1 cut(s) | 601 |
| Bsp1286I | GDGCHC | 2 cut(s) | 64, 174 |
| Bsp143I | GATC | 3 cut(s) | 103, 256, 646 |
| BspACI | CCGC | 1 cut(s) | 273 |
| BspANI | GGCC | 1 cut(s) | 601 |
| BspLI | GGNNCC | 1 cut(s) | 159 |
| BspPI | GGATC | 2 cut(s) | 251, 654 |
| BspQI | GCTCTTC | 1 cut(s) | 399 |
| BsrI | ACTGG | 3 cut(s) | 161, 339, 602 |
| BssECI | CCNNGG | 1 cut(s) | 378 |
| BssMI | GATC | 3 cut(s) | 103, 256, 646 |
| BssT1I | CCWWGG | 1 cut(s) | 378 |
| Bst4CI | ACNGT | 1 cut(s) | 282 |
| Bst6I | CTCTTC | 2 cut(s) | 399, 528 |
| BstC8I | GCNNGC | 1 cut(s) | 474 |
| BstF5I | GGATG | 1 cut(s) | 473 |
| BstHHI | GCGC | 1 cut(s) | 478 |
| BstKTI | GATC | 3 cut(s) | 106, 259, 649 |
| BstMAI | GTCTC | 1 cut(s) | 603 |
| BstMBI | GATC | 3 cut(s) | 103, 256, 646 |
| BstSLI | GKGCMC | 2 cut(s) | 64, 174 |
| BstV1I | GCAGC | 2 cut(s) | 234, 262 |
| BstV2I | GAAGAC | 1 cut(s) | 507 |
| BstX2I | RGATCY | 2 cut(s) | 103, 256 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 324 |
| BstYI | RGATCY | 2 cut(s) | 103, 256 |
| BsuRI | GGCC | 1 cut(s) | 601 |
| BtsCI | GGATG | 1 cut(s) | 473 |
| BtsIMutI | CAGTG | 3 cut(s) | 62, 168, 346 |
| Cac8I | GCNNGC | 1 cut(s) | 474 |
| CfoI | GCGC | 1 cut(s) | 478 |
| Csp6I | GTAC | 1 cut(s) | 394 |
| CviAII | CATG | 2 cut(s) | 27, 555 |
| CviQI | GTAC | 1 cut(s) | 394 |
| DpnI | GATC | 3 cut(s) | 105, 258, 648 |
| DpnII | GATC | 3 cut(s) | 103, 256, 646 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 617 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 617 |
| EaeI | YGGCCR | 1 cut(s) | 599 |
| Eam1104I | CTCTTC | 2 cut(s) | 399, 528 |
| EarI | CTCTTC | 2 cut(s) | 399, 528 |
| Eco130I | CCWWGG | 1 cut(s) | 378 |
| Eco57I | CTGAAG | 2 cut(s) | 116, 273 |
| EcoT14I | CCWWGG | 1 cut(s) | 378 |
| ErhI | CCWWGG | 1 cut(s) | 378 |
| FaeI | CATG | 2 cut(s) | 30, 558 |
| FaiI | YATR | 9 cut(s) | 28, 85, 87, 99, 138, 150, 483, 556, 584 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 246, 278, 371, 403, 410, 442 |
| FaqI | GGGAC | 1 cut(s) | 65 |
| FatI | CATG | 2 cut(s) | 26, 554 |
| FauNDI | CATATG | 1 cut(s) | 85 |
| Fnu4HI | GCNGC | 3 cut(s) | 248, 273, 276 |
| FokI | GGATG | 1 cut(s) | 480 |
| Fsp4HI | GCNGC | 3 cut(s) | 248, 273, 276 |
| FspBI | CTAG | 1 cut(s) | 606 |
| FspI | TGCGCA | 1 cut(s) | 477 |
| GlaI | GCGC | 1 cut(s) | 477 |
| GluI | GCNGC | 3 cut(s) | 248, 273, 276 |
| HaeIII | GGCC | 1 cut(s) | 601 |
| HapII | CCGG | 1 cut(s) | 510 |
| HhaI | GCGC | 1 cut(s) | 478 |
| Hin1II | CATG | 2 cut(s) | 30, 558 |
| Hin6I | GCGC | 1 cut(s) | 476 |
| HinP1I | GCGC | 1 cut(s) | 476 |
| HincII | GTYRAC | 1 cut(s) | 525 |
| HindII | GTYRAC | 1 cut(s) | 525 |
| HindIII | AAGCTT | 1 cut(s) | 440 |
| HinfI | GANTC | 2 cut(s) | 194, 329 |
| HpaI | GTTAAC | 1 cut(s) | 525 |
| HpaII | CCGG | 1 cut(s) | 510 |
| HphI | GGTGA | 2 cut(s) | 311, 641 |
| Hpy166II | GTNNAC | 2 cut(s) | 172, 525 |
| Hpy188III | TCNNGA | 4 cut(s) | 209, 263, 606, 641 |
| Hpy8I | GTNNAC | 2 cut(s) | 172, 525 |
| HpyAV | CCTTC | 1 cut(s) | 226 |
| HpyCH4III | ACNGT | 1 cut(s) | 282 |
| HpyCH4IV | ACGT | 1 cut(s) | 504 |
| HpyCH4V | TGCA | 4 cut(s) | 172, 278, 461, 572 |
| HpySE526I | ACGT | 1 cut(s) | 504 |
| Hsp92II | CATG | 2 cut(s) | 30, 558 |
| HspAI | GCGC | 1 cut(s) | 476 |
| KspAI | GTTAAC | 1 cut(s) | 525 |
| Kzo9I | GATC | 3 cut(s) | 103, 256, 646 |
| LguI | GCTCTTC | 1 cut(s) | 399 |
| LmnI | GCTCC | 1 cut(s) | 583 |
| LpnPI | CCDG | 6 cut(s) | 174, 264, 352, 523, 583, 617 |
| Lsp1109I | GCAGC | 2 cut(s) | 234, 262 |
| LweI | GCATC | 1 cut(s) | 565 |
| MaeI | CTAG | 1 cut(s) | 606 |
| MaeII | ACGT | 1 cut(s) | 504 |
| MaeIII | GTNAC | 1 cut(s) | 505 |
| MalI | GATC | 3 cut(s) | 105, 258, 648 |
| MboI | GATC | 3 cut(s) | 103, 256, 646 |
| MboII | GAAGA | 8 cut(s) | 113, 122, 266, 413, 416, 512, 542, 545 |
| MfeI | CAATTG | 1 cut(s) | 186 |
| MflI | RGATCY | 2 cut(s) | 103, 256 |
| MhlI | GDGCHC | 2 cut(s) | 64, 174 |
| MlsI | TGGCCA | 1 cut(s) | 601 |
| MluCI | AATT | 5 cut(s) | 186, 293, 422, 518, 626 |
| MluNI | TGGCCA | 1 cut(s) | 601 |
| MlyI | GAGTC | 2 cut(s) | 203, 338 |
| MnlI | CCTC | 3 cut(s) | 270, 352, 637 |
| Mox20I | TGGCCA | 1 cut(s) | 601 |
| MroXI | GAANNNNTTC | 1 cut(s) | 537 |
| MscI | TGGCCA | 1 cut(s) | 601 |
| MseI | TTAA | 1 cut(s) | 524 |
| MslI | CAYNNNNRTG | 2 cut(s) | 466, 670 |
| Msp20I | TGGCCA | 1 cut(s) | 601 |
| MspA1I | CMGCKG | 1 cut(s) | 275 |
| MspI | CCGG | 1 cut(s) | 510 |
| MunI | CAATTG | 1 cut(s) | 186 |
| NdeI | CATATG | 1 cut(s) | 85 |
| NdeII | GATC | 3 cut(s) | 103, 256, 646 |
| NlaIII | CATG | 2 cut(s) | 30, 558 |
| NlaIV | GGNNCC | 1 cut(s) | 159 |
| NsbI | TGCGCA | 1 cut(s) | 477 |
| PciSI | GCTCTTC | 1 cut(s) | 399 |
| PdmI | GAANNNNTTC | 1 cut(s) | 537 |
| PflFI | GACNNNGTC | 1 cut(s) | 28 |
| PkrI | GCNGC | 3 cut(s) | 249, 274, 277 |
| PleI | GAGTC | 2 cut(s) | 202, 337 |
| PpsI | GAGTC | 2 cut(s) | 202, 337 |
| PspN4I | GGNNCC | 1 cut(s) | 159 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 377, 409 |
| PsuI | RGATCY | 2 cut(s) | 103, 256 |
| PsyI | GACNNNGTC | 1 cut(s) | 28 |
| RsaI | GTAC | 1 cut(s) | 395 |
| RsaNI | GTAC | 1 cut(s) | 394 |
| RseI | CAYNNNNRTG | 2 cut(s) | 466, 670 |
| SapI | GCTCTTC | 1 cut(s) | 399 |
| SaqAI | TTAA | 1 cut(s) | 524 |
| SatI | GCNGC | 3 cut(s) | 248, 273, 276 |
| Sau3AI | GATC | 3 cut(s) | 103, 256, 646 |
| ScaI | AGTACT | 1 cut(s) | 395 |
| SchI | GAGTC | 2 cut(s) | 203, 338 |
| SduI | GDGCHC | 2 cut(s) | 64, 174 |
| SetI | ASST | 9 cut(s) | 18, 79, 159, 312, 367, 411, 444, 507, 654 |
| SfaNI | GCATC | 1 cut(s) | 565 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 466, 670 |
| SmlI | CTYRAG | 1 cut(s) | 263 |
| SmoI | CTYRAG | 1 cut(s) | 263 |
| Sse9I | AATT | 5 cut(s) | 186, 293, 422, 518, 626 |
| SsiI | CCGC | 1 cut(s) | 273 |
| SspMI | CTAG | 1 cut(s) | 606 |
| StyI | CCWWGG | 1 cut(s) | 378 |
| TaaI | ACNGT | 1 cut(s) | 282 |
| TaiI | ACGT | 1 cut(s) | 507 |
| TasI | AATT | 5 cut(s) | 186, 293, 422, 518, 626 |
| TatI | WGTACW | 1 cut(s) | 393 |
| TauI | GCSGC | 1 cut(s) | 275 |
| Tru1I | TTAA | 1 cut(s) | 524 |
| Tru9I | TTAA | 1 cut(s) | 524 |
| TscAI | CASTG | 3 cut(s) | 69, 168, 346 |
| TseI | GCWGC | 2 cut(s) | 247, 275 |
| TspDTI | ATGAA | 5 cut(s) | 102, 114, 137, 237, 530 |
| TspGWI | ACGGA | 1 cut(s) | 531 |
| TspRI | CASTG | 3 cut(s) | 69, 168, 346 |
| Tth111I | GACNNNGTC | 1 cut(s) | 28 |
| VneI | GTGCAC | 1 cut(s) | 170 |
| XapI | RAATTY | 3 cut(s) | 293, 422, 626 |
| XbaI | TCTAGA | 1 cut(s) | 605 |
| XmnI | GAANNNNTTC | 1 cut(s) | 537 |
| XspI | CTAG | 1 cut(s) | 606 |
| ZrmI | AGTACT | 1 cut(s) | 395 |