Glutathione S-transferase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 280 |
| AciI | CCGC | 1 cut(s) | 280 |
| AclWI | GGATC | 2 cut(s) | 199, 634 |
| AcsI | RAATTY | 2 cut(s) | 103, 326 |
| AfaI | GTAC | 3 cut(s) | 165, 215, 516 |
| AgsI | TTSAA | 3 cut(s) | 385, 494, 651 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 486, 518 |
| AluBI | AGCT | 4 cut(s) | 26, 70, 404, 578 |
| AluI | AGCT | 4 cut(s) | 26, 70, 404, 578 |
| Alw21I | GWGCWC | 1 cut(s) | 72 |
| Alw26I | GTCTC | 1 cut(s) | 218 |
| AlwI | GGATC | 2 cut(s) | 199, 634 |
| Ama87I | CYCGRG | 1 cut(s) | 209 |
| AoxI | GGCC | 3 cut(s) | 29, 230, 653 |
| ApeKI | GCWGC | 1 cut(s) | 359 |
| ApoI | RAATTY | 2 cut(s) | 103, 326 |
| AspLEI | GCGC | 1 cut(s) | 280 |
| AspS9I | GGNCC | 1 cut(s) | 231 |
| AsuHPI | GGTGA | 1 cut(s) | 181 |
| AvaI | CYCGRG | 1 cut(s) | 209 |
| BanII | GRGCYC | 2 cut(s) | 64, 72 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 74, 106 |
| Bbv12I | GWGCWC | 1 cut(s) | 72 |
| BbvI | GCAGC | 1 cut(s) | 346 |
| BccI | CCATC | 2 cut(s) | 332, 603 |
| BcoDI | GTCTC | 1 cut(s) | 218 |
| BfaI | CTAG | 2 cut(s) | 33, 65 |
| BglII | AGATCT | 1 cut(s) | 387 |
| BisI | GCNGC | 1 cut(s) | 360 |
| BlsI | GCNGC | 1 cut(s) | 361 |
| BmcAI | AGTACT | 1 cut(s) | 516 |
| BmeT110I | CYCGRG | 1 cut(s) | 209 |
| BmgT120I | GGNCC | 1 cut(s) | 231 |
| BmiI | GGNNCC | 1 cut(s) | 233 |
| BmrI | ACTGGG | 1 cut(s) | 137 |
| BmuI | ACTGGG | 1 cut(s) | 137 |
| BpuEI | CTTGAG | 1 cut(s) | 56 |
| BsaAI | YACGTR | 1 cut(s) | 94 |
| BsaWI | WCCGGW | 1 cut(s) | 160 |
| Bse1I | ACTGG | 1 cut(s) | 143 |
| Bse3DI | GCAATG | 1 cut(s) | 439 |
| BseGI | GGATG | 1 cut(s) | 595 |
| BseMI | GCAATG | 1 cut(s) | 439 |
| BseNI | ACTGG | 1 cut(s) | 143 |
| BseRI | GAGGAG | 4 cut(s) | 236, 428, 466, 643 |
| BseXI | GCAGC | 1 cut(s) | 346 |
| BseYI | CCCAGC | 1 cut(s) | 574 |
| Bsh1236I | CGCG | 1 cut(s) | 280 |
| BshFI | GGCC | 3 cut(s) | 31, 232, 655 |
| BsiHKAI | GWGCWC | 1 cut(s) | 72 |
| BsiHKCI | CYCGRG | 1 cut(s) | 209 |
| BsiSI | CCGG | 1 cut(s) | 161 |
| BsmAI | GTCTC | 1 cut(s) | 218 |
| BsnI | GGCC | 3 cut(s) | 31, 232, 655 |
| BsoBI | CYCGRG | 1 cut(s) | 209 |
| Bsp1286I | GDGCHC | 2 cut(s) | 64, 72 |
| Bsp143I | GATC | 3 cut(s) | 204, 387, 639 |
| BspACI | CCGC | 1 cut(s) | 280 |
| BspANI | GGCC | 3 cut(s) | 31, 232, 655 |
| BspFNI | CGCG | 1 cut(s) | 280 |
| BspHI | TCATGA | 1 cut(s) | 262 |
| BspLI | GGNNCC | 1 cut(s) | 233 |
| BspPI | GGATC | 2 cut(s) | 199, 634 |
| BspQI | GCTCTTC | 1 cut(s) | 390 |
| BsrDI | GCAATG | 1 cut(s) | 439 |
| BsrI | ACTGG | 1 cut(s) | 143 |
| BssMI | GATC | 3 cut(s) | 204, 387, 639 |
| Bst6I | CTCTTC | 1 cut(s) | 390 |
| BstBAI | YACGTR | 1 cut(s) | 94 |
| BstC8I | GCNNGC | 2 cut(s) | 402, 522 |
| BstDEI | CTNAG | 1 cut(s) | 391 |
| BstEII | GGTNACC | 1 cut(s) | 312 |
| BstF5I | GGATG | 1 cut(s) | 595 |
| BstFNI | CGCG | 1 cut(s) | 280 |
| BstHHI | GCGC | 1 cut(s) | 280 |
| BstKTI | GATC | 3 cut(s) | 207, 390, 642 |
| BstMAI | GTCTC | 1 cut(s) | 218 |
| BstMBI | GATC | 3 cut(s) | 204, 387, 639 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 455 |
| BstNSI | RCATGY | 1 cut(s) | 524 |
| BstPI | GGTNACC | 1 cut(s) | 312 |
| BstSNI | TACGTA | 1 cut(s) | 94 |
| BstUI | CGCG | 1 cut(s) | 280 |
| BstV1I | GCAGC | 1 cut(s) | 346 |
| BstX2I | RGATCY | 1 cut(s) | 387 |
| BstYI | RGATCY | 1 cut(s) | 387 |
| BsuRI | GGCC | 3 cut(s) | 31, 232, 655 |
| BtsCI | GGATG | 1 cut(s) | 595 |
| Cac8I | GCNNGC | 2 cut(s) | 402, 522 |
| CciI | TCATGA | 1 cut(s) | 262 |
| CfoI | GCGC | 1 cut(s) | 280 |
| Cfr13I | GGNCC | 1 cut(s) | 231 |
| Csp6I | GTAC | 3 cut(s) | 164, 214, 515 |
| CviAII | CATG | 3 cut(s) | 263, 350, 521 |
| CviQI | GTAC | 3 cut(s) | 164, 214, 515 |
| DdeI | CTNAG | 1 cut(s) | 391 |
| DpnI | GATC | 3 cut(s) | 206, 389, 641 |
| DpnII | GATC | 3 cut(s) | 204, 387, 639 |
| Eam1104I | CTCTTC | 1 cut(s) | 390 |
| EarI | CTCTTC | 1 cut(s) | 390 |
| Ecl136II | GAGCTC | 1 cut(s) | 70 |
| Eco105I | TACGTA | 1 cut(s) | 94 |
| Eco147I | AGGCCT | 1 cut(s) | 655 |
| Eco24I | GRGCYC | 2 cut(s) | 64, 72 |
| Eco53kI | GAGCTC | 1 cut(s) | 70 |
| Eco88I | CYCGRG | 1 cut(s) | 209 |
| Eco91I | GGTNACC | 1 cut(s) | 312 |
| EcoICRI | GAGCTC | 1 cut(s) | 70 |
| EcoO65I | GGTNACC | 1 cut(s) | 312 |
| EcoT38I | GRGCYC | 2 cut(s) | 64, 72 |
| FaeI | CATG | 3 cut(s) | 266, 353, 524 |
| FatI | CATG | 3 cut(s) | 262, 349, 520 |
| Fnu4HI | GCNGC | 1 cut(s) | 360 |
| FokI | GGATG | 1 cut(s) | 582 |
| FriOI | GRGCYC | 2 cut(s) | 64, 72 |
| Fsp4HI | GCNGC | 1 cut(s) | 360 |
| FspBI | CTAG | 2 cut(s) | 33, 65 |
| GlaI | GCGC | 1 cut(s) | 279 |
| GluI | GCNGC | 1 cut(s) | 360 |
| GsaI | CCCAGC | 1 cut(s) | 578 |
| HaeIII | GGCC | 3 cut(s) | 31, 232, 655 |
| HapII | CCGG | 1 cut(s) | 161 |
| HhaI | GCGC | 1 cut(s) | 280 |
| Hin1II | CATG | 3 cut(s) | 266, 353, 524 |
| Hin6I | GCGC | 1 cut(s) | 278 |
| HinP1I | GCGC | 1 cut(s) | 278 |
| HinfI | GANTC | 3 cut(s) | 157, 194, 238 |
| HpaII | CCGG | 1 cut(s) | 161 |
| HphI | GGTGA | 1 cut(s) | 181 |
| Hpy166II | GTNNAC | 2 cut(s) | 148, 172 |
| Hpy188I | TCNGA | 3 cut(s) | 50, 253, 374 |
| Hpy188III | TCNNGA | 5 cut(s) | 65, 263, 286, 385, 599 |
| Hpy8I | GTNNAC | 2 cut(s) | 148, 172 |
| HpyAV | CCTTC | 1 cut(s) | 349 |
| HpyCH4IV | ACGT | 1 cut(s) | 93 |
| HpyCH4V | TGCA | 3 cut(s) | 133, 362, 520 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 455 |
| HpyF3I | CTNAG | 1 cut(s) | 391 |
| HpySE526I | ACGT | 1 cut(s) | 93 |
| Hsp92II | CATG | 3 cut(s) | 266, 353, 524 |
| HspAI | GCGC | 1 cut(s) | 278 |
| Kzo9I | GATC | 3 cut(s) | 204, 387, 639 |
| LguI | GCTCTTC | 1 cut(s) | 390 |
| LmnI | GCTCC | 1 cut(s) | 23 |
| LpnPI | CCDG | 7 cut(s) | 156, 174, 248, 271, 392, 588, 612 |
| Lsp1109I | GCAGC | 1 cut(s) | 346 |
| MaeI | CTAG | 2 cut(s) | 33, 65 |
| MaeII | ACGT | 1 cut(s) | 93 |
| MaeIII | GTNAC | 1 cut(s) | 312 |
| MalI | GATC | 3 cut(s) | 206, 389, 641 |
| MboI | GATC | 3 cut(s) | 204, 387, 639 |
| MboII | GAAGA | 3 cut(s) | 17, 356, 407 |
| MflI | RGATCY | 1 cut(s) | 387 |
| MhlI | GDGCHC | 2 cut(s) | 64, 72 |
| MluCI | AATT | 4 cut(s) | 43, 103, 326, 547 |
| MlyI | GAGTC | 1 cut(s) | 203 |
| MseI | TTAA | 2 cut(s) | 324, 555 |
| MslI | CAYNNNNRTG | 1 cut(s) | 525 |
| MspI | CCGG | 1 cut(s) | 161 |
| MvnI | CGCG | 1 cut(s) | 280 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 455 |
| NdeII | GATC | 3 cut(s) | 204, 387, 639 |
| NlaIII | CATG | 3 cut(s) | 266, 353, 524 |
| NlaIV | GGNNCC | 1 cut(s) | 233 |
| NspI | RCATGY | 1 cut(s) | 524 |
| PaeI | GCATGC | 1 cut(s) | 524 |
| PaeR7I | CTCGAG | 1 cut(s) | 209 |
| PagI | TCATGA | 1 cut(s) | 262 |
| PceI | AGGCCT | 1 cut(s) | 655 |
| PciSI | GCTCTTC | 1 cut(s) | 390 |
| PfeI | GAWTC | 2 cut(s) | 157, 238 |
| PkrI | GCNGC | 1 cut(s) | 361 |
| PleI | GAGTC | 1 cut(s) | 202 |
| PpsI | GAGTC | 1 cut(s) | 202 |
| Ppu21I | YACGTR | 1 cut(s) | 94 |
| Psp124BI | GAGCTC | 1 cut(s) | 72 |
| PspEI | GGTNACC | 1 cut(s) | 312 |
| PspFI | CCCAGC | 1 cut(s) | 574 |
| PspN4I | GGNNCC | 1 cut(s) | 233 |
| PspPI | GGNCC | 1 cut(s) | 231 |
| PspXI | VCTCGAGB | 1 cut(s) | 209 |
| PsuI | RGATCY | 1 cut(s) | 387 |
| RsaI | GTAC | 3 cut(s) | 165, 215, 516 |
| RsaNI | GTAC | 3 cut(s) | 164, 214, 515 |
| RseI | CAYNNNNRTG | 1 cut(s) | 525 |
| SacI | GAGCTC | 1 cut(s) | 72 |
| SapI | GCTCTTC | 1 cut(s) | 390 |
| SaqAI | TTAA | 2 cut(s) | 324, 555 |
| SatI | GCNGC | 1 cut(s) | 360 |
| Sau3AI | GATC | 3 cut(s) | 204, 387, 639 |
| Sau96I | GGNCC | 1 cut(s) | 231 |
| ScaI | AGTACT | 1 cut(s) | 516 |
| SchI | GAGTC | 1 cut(s) | 203 |
| SduI | GDGCHC | 2 cut(s) | 64, 72 |
| SetI | ASST | 5 cut(s) | 28, 72, 96, 406, 580 |
| Sfr274I | CTCGAG | 1 cut(s) | 209 |
| SlaI | CTCGAG | 1 cut(s) | 209 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 525 |
| SmlI | CTYRAG | 2 cut(s) | 71, 209 |
| SmoI | CTYRAG | 2 cut(s) | 71, 209 |
| SnaBI | TACGTA | 1 cut(s) | 94 |
| SphI | GCATGC | 1 cut(s) | 524 |
| Sse9I | AATT | 4 cut(s) | 43, 103, 326, 547 |
| SseBI | AGGCCT | 1 cut(s) | 655 |
| SsiI | CCGC | 1 cut(s) | 280 |
| SspMI | CTAG | 2 cut(s) | 33, 65 |
| SstI | GAGCTC | 1 cut(s) | 72 |
| StuI | AGGCCT | 1 cut(s) | 655 |
| TaiI | ACGT | 1 cut(s) | 96 |
| TaqI | TCGA | 1 cut(s) | 210 |
| TasI | AATT | 4 cut(s) | 43, 103, 326, 547 |
| TatI | WGTACW | 2 cut(s) | 213, 514 |
| TfiI | GAWTC | 2 cut(s) | 157, 238 |
| Tru1I | TTAA | 2 cut(s) | 324, 555 |
| Tru9I | TTAA | 2 cut(s) | 324, 555 |
| TseI | GCWGC | 1 cut(s) | 359 |
| TspDTI | ATGAA | 1 cut(s) | 102 |
| XapI | RAATTY | 2 cut(s) | 103, 326 |
| XbaI | TCTAGA | 1 cut(s) | 64 |
| XceI | RCATGY | 1 cut(s) | 524 |
| XhoI | CTCGAG | 1 cut(s) | 209 |
| XspI | CTAG | 2 cut(s) | 33, 65 |
| ZrmI | AGTACT | 1 cut(s) | 516 |