Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0001033

Physical Location & Seq

Genomic Coordinates Forward (+)

21491672 .. 21497934

Gene Structure 6263 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1087900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2283 bp

ATGGGACACGGTGATAAAGGAGGCAGACCCAGTAAAAAACCCAAATTCTCCGCCAAGGAACTTGCTCACGTGGAAGACGACGACCCATATTACGGCGGCGAAGATGTCGACGATGATCCTCGCGACGGTGAAGGGAAAAAGAGGGATTTTACCAAATTAGAACTGAAACCTGACCACGCCAATCGGCCTTTATGGGCTTGCGCTGATGGCCGGATATTCCTCGAAACCTTCTCTGCTTTGTACAAACAAGCTTATGATTTCCTAATTGCCATTGCGGAACCCGTTTGCAGGCCAGAGTCGATGCACGAGTACAATTTGACCCCTCATTCGTTGTACGCGGCTGTGTCAGTCGGTCTTGAGACAGAAACCATCATTTCTGTTTTGAATAAACTGTCAAAGACCAAGCTCCCTAAAGAAATGATTGAATTTATACACGGTTCTACTGCCAATTACGGCAAAGTGAAGCTCGTTCTTAAGAAGAATAAGTATTTTGTGTTGAAGACATTGCTTAAGGACGAAGTCATATCTAGAGCAAGGCTTAACTCTGAGGGTTCTCGTGGAGCTGATGATTTTACCATTAGCAAAACAATGGGTGAAATTGGAGCTGGTCCCAGTGATTTGCTAAATGAAGCAGAACTGGCAGCTGCAGCTGAAGAAAAAGAAACTCATGCATTTGAAATTGATCCTTCACAGGTTGAAAATGTTAAGGAACGTTGCTTGCCAAATGCTTTGAACTATCCCATGTTGGAGGAGTATGACTTCAGAAATGATACAGTTAACCCTGATCTAGATATGGAACTAAAGCCTCAGGCTCAACCACGACCTTATCAGGAGAAGAGCTTGAGTAAAATGTTTGGGAATGGGAGAGCAAGATCTGGCATTATTGTGCTACCCTGTGGTGCCGGAAAATCTCTAGTTGGTGTTTCTGCAGCTTCCCGGATAAGAAAGAGCTGCCTTTGTTTAGCAACAAATGCTGTCTCTGTTGATCAGTGGGCTTTTCAGTTCAAGTTGTGGTCAACAATCCGCGATGATCAAATTTGTCGTTTCACATCTGATAGTAAAGAAAGGTTCCGTGGAAATGCTGGGGTGGTTGTAACAACATATAACATGGTAGCCTTTGGTGGAAAGAGGTCAGAAGAATCTGAAAAGATTATTGAAGAAATAAGAAATAGAGAATGGGGATTGCTTCTCATGGACGAGGTGCACGTTGTTCCTGCTCAAATGTTTCGGAAGGTCATTAGCATTACTAAGTCTCACTGCAAGCTTGGGCTCACTGCCACACTTGTGAGAGAGGATGAGAGGATCACAGATTTGAACTTCCTTATTGGTCCGAAATTGTATGAAGCAAACTGGTTGGATTTGGTAAAAGGTGGATTTATTGCAAATGTACAGTGCGCTGAAGTATGGTGTCCAATGACAAAAGAGTTCTTTGCAGAATATTTGAAGAAAGAGAACTCCAAGAAAAAGCAGGCACTTTATGTGATGAATCCAAATAAGTTTAGGGCATGCGAGTTTCTTATACGTTTCCATGAACAGCAACGTGGAGATAAAATTATCGTCTTTGCTGACAATCTTTTTGCGCTTGTGGAGTATGCAATGAAACTTCGGAAACCTATGATCTATGGTGCCACCAGCCATGTTGAGAGGACAAAAATCCTAGAAGCTTTTAAAACAAGTCGTGACGTAAACACTATATTCCTGTCTAAGGTCGGTGATAACTCCATAGATATTCCTGAGGCAAATGTGATAATTCAGATCTCGTCACATGCTGGTTCAAGACGTCAAGAAGCGCAGCGTCTTGGTCGTATTCTGAGGGCTAAGGGTAGACTTCAGGACCGGATGGCAGGAGGTAAAGAGGAGTACAATGCATTCTTTTATTCCCTCGTATCAATGGACACACAGGAAATGTACTATTCAACTAAGCGGCAGCAATTTTTGATTGATCAAGGTTATAGTTTCAAGATTATTACAAGCTTGCCCCCGCCTGATTCAGGCCCTGAATTGAACTACCACCATCTTAATGACCAACTGTCACTACTCTCCAAGGTGTTGAGTGCTGGTGATGATGCAGTTGGTTTGGAGCAACTAGAAGAAGATGCCGATGACATTGCCCTTCATAAAGCTCGCCGTTTCAGTGGATCCATGAGTGCTATGTCGGGTGCAAATGGGATGGTTTACATGGAATACAGCACTGGGAGAAACAAATTTGGCAAAGGCCAGATCAAGGGTAAGCCCAAGGATCCAGCCAAGCGACATCATTTGTTTAAAAAACGCTTTACCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0008150 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0010224 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0050896

KEGG Pathways

Metabolic & Signaling

ko03022 ko03420 map03022 map03420

Pfam Domains

Protein Families

ERCC3_RAD25_C Helicase_C_3 ResIII

Protein Analysis

761

Amino Acids

85.78

Weight (kDa)

8.47

Isoelectric Point (pI)

32.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Helicase_C_3	PF13625	64 - 166	4.3e-28	Helicase conserved C-terminal domain
ResIII	PF04851	272 - 429	7.6e-18	Type III restriction enzyme, res subunit
ERCC3_RAD25_C	PF16203	454 - 700	5.7e-116	ERCC3/RAD25/XPB C-terminal helicase
Helicase_C	PF00271	504 - 606	1.3e-12	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0001033)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G41360 AT5G41360 AT5G41370
fragaria_vesca	FvH4_5g02840 FvH4_5g02840 FvH4_5g02840 FvH4_5g02840 FvH4_5g02840 FvH4_5g19420 FvH4_5g19430 FvH4_7g08220 FvH4_7g08220
malus_domestica	MD06G1087900.v1.1
prunus_persica	Prupe.5G104000_v2.0.a1
pyrus_communis	pycom06g08400
rosa_chinensis	RchiOBHm_Chr1g0341611 RchiOBHm_Chr1g0341621 RchiOBHm_Chr1g0341861 RchiOBHm_Chr7g0197061 RchiOBHm_Chr7g0203931
rosa_laevigata	RLG00000003497 RLG00000003962 RLG00000029105 RLG00000029132
rosa_multiflora	Rmu_co8328521.1_g000001 Rmu_co8329503.1_g000001 Rmu_sc0002302.1_g000034 Rmu_sc0002322.1_g000070 Rmu_sc0003411.1_g000004 Rmu_sc0013871.1_g000001 Rmu_sc0032022.1_g000001
rosa_roxburghii	Rroxscaffold_3G00258690 Rroxscaffold_4G00312270 Rroxscaffold_4G00312590
rosa_rugosa	Rorug01G0154800.1 Rorug07G0037400 Rorug07G0079500
rosa_samantha	Rh1AG168500 Rh1AG170500 Rh1BG136800 Rh1BG138300 Rh1BG138400 Rh1CG156100 Rh1CG158700 Rh1DG170300 Rh1DG170600 Rh1DG170700 Rh7AG162300 Rh7AG209400 Rh7AG209500 Rh7BG164400 Rh7BG208200 Rh7BG208300 Rh7CG170500 Rh7CG221900 Rh7DG163800 Rh7DG216500
rosa_wichuraiana	Rw1G014160 Rw1G014180 Rw7G014170 Rw7G018250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1783
AccB1I	GGYRCC	2 cut(s)	899, 1625
AccI	GTMKAC	2 cut(s)	108, 1825
AccII	CGCG	3 cut(s)	123, 338, 1026
AciI	CCGC	7 cut(s)	51, 96, 275, 338, 1024, 1924, 1982
AclI	AACGTT	1 cut(s)	712
AclWI	GGATC	7 cut(s)	110, 677, 1310, 2133, 2146, 2234, 2247
AcoI	YGGCCR	1 cut(s)	208
AcsI	RAATTY	4 cut(s)	44, 425, 1035, 2204
AcuI	CTGAAG	4 cut(s)	672, 745, 1419, 1814
AcvI	CACGTG	1 cut(s)	70
AcyI	GRCGYC	1 cut(s)	1780
AfaI	GTAC	6 cut(s)	242, 311, 335, 1389, 1862, 1910
AfiI	CCNNNNNNNGG	6 cut(s)	92, 125, 288, 1705, 1991, 2224
AflII	CTTAAG	2 cut(s)	473, 509
AhdI	GACNNNNNGTC	1 cut(s)	2029
AleI	CACNNNNGTG	1 cut(s)	1283
Alw21I	GWGCWC	1 cut(s)	1206
Alw26I	GTCTC	3 cut(s)	353, 982, 1257
Alw44I	GTGCAC	1 cut(s)	1202
AlwI	GGATC	7 cut(s)	110, 677, 1310, 2133, 2146, 2234, 2247
AlwNI	CAGNNNCTG	1 cut(s)	1997
AoxI	GGCC	5 cut(s)	185, 208, 290, 1993, 2215
ApaLI	GTGCAC	1 cut(s)	1202
ApeKI	GCWGC	7 cut(s)	641, 644, 647, 929, 951, 1792, 1927
ApoI	RAATTY	4 cut(s)	44, 425, 1035, 2204
ArsI	GACNNNNNNTTYG	4 cut(s)	310, 342, 1559, 1591
AspLEI	GCGC	4 cut(s)	203, 1397, 1582, 1792
AspS9I	GGNCC	4 cut(s)	608, 1328, 1834, 1994
AsuC2I	CCSGG	1 cut(s)	937
AsuHPI	GGTGA	5 cut(s)	23, 140, 605, 1724, 2072
AvaII	GGWCC	3 cut(s)	608, 1328, 1834
AxyI	CCTNAGG	2 cut(s)	807, 1734
BaeGI	GKGCMC	1 cut(s)	1206
BamHI	GGATCC	2 cut(s)	2138, 2239
BanI	GGYRCC	2 cut(s)	899, 1625
BanII	GRGCYC	1 cut(s)	1272
BauI	CACGAG	2 cut(s)	305, 555
BbrPI	CACGTG	1 cut(s)	70
BbsI	GAAGAC	2 cut(s)	81, 506
Bbv12I	GWGCWC	1 cut(s)	1206
BbvI	GCAGC	7 cut(s)	631, 653, 659, 938, 941, 1804, 1939
BccI	CCATC	5 cut(s)	200, 377, 1834, 2022, 2164
BceAI	ACGGC	3 cut(s)	109, 469, 2112
BcgI	CGANNNNNNTGC	2 cut(s)	2090, 2124
BclI	TGATCA	3 cut(s)	985, 1030, 1942
BcnI	CCSGG	1 cut(s)	937
BcoDI	GTCTC	3 cut(s)	353, 982, 1257
BfaI	CTAG	5 cut(s)	528, 788, 914, 1658, 2087
BfmI	CTRYAG	2 cut(s)	645, 927
BfrI	CTTAAG	2 cut(s)	473, 509
BglII	AGATCT	2 cut(s)	872, 1755
Bme1390I	CCNGG	1 cut(s)	937
Bme18I	GGWCC	3 cut(s)	608, 1328, 1834
BmeRI	GACNNNNNGTC	1 cut(s)	2029
BmgT120I	GGNCC	4 cut(s)	608, 1328, 1834, 1994
BmiI	GGNNCC	7 cut(s)	279, 610, 901, 1070, 1627, 2140, 2241
BmrFI	CCNGG	1 cut(s)	937
BmrI	ACTGGG	3 cut(s)	24, 606, 2202
BmsI	GCATC	3 cut(s)	291, 2056, 2086
BmuI	ACTGGG	3 cut(s)	24, 606, 2202
BpiI	GAAGAC	2 cut(s)	81, 506
Bpu10I	CCTNAGC	1 cut(s)	1818
BpuEI	CTTGAG	2 cut(s)	377, 862
BpuMI	CCSGG	1 cut(s)	937
BsaAI	YACGTR	1 cut(s)	70
BsaHI	GRCGYC	1 cut(s)	1780
BsaJI	CCNNGG	4 cut(s)	54, 1072, 2043, 2235
BsaWI	WCCGGW	1 cut(s)	1836
Bsc4I	CCNNNNNNNGG	6 cut(s)	92, 125, 288, 1705, 1991, 2224
Bse1I	ACTGG	5 cut(s)	30, 612, 642, 1355, 2197
Bse21I	CCTNAGG	2 cut(s)	807, 1734
Bse3DI	GCAATG	4 cut(s)	270, 503, 1602, 2106
BseDI	CCNNGG	4 cut(s)	54, 1072, 2043, 2235
BseGI	GGATG	3 cut(s)	1300, 1845, 2175
BseLI	CCNNNNNNNGG	6 cut(s)	92, 125, 288, 1705, 1991, 2224
BseMI	GCAATG	4 cut(s)	270, 503, 1602, 2106
BseMII	CTCAG	4 cut(s)	537, 821, 1725, 1802
BseNI	ACTGG	5 cut(s)	30, 612, 642, 1355, 2197
BseRI	GAGGAG	2 cut(s)	764, 1871
BseSI	GKGCMC	1 cut(s)	1206
BseXI	GCAGC	7 cut(s)	631, 653, 659, 938, 941, 1804, 1939
BseYI	CCCAGC	1 cut(s)	1082
Bsh1236I	CGCG	3 cut(s)	123, 338, 1026
BshFI	GGCC	5 cut(s)	187, 210, 292, 1995, 2217
BshNI	GGYRCC	2 cut(s)	899, 1625
BsiHKAI	GWGCWC	1 cut(s)	1206
BsiSI	CCGG	4 cut(s)	211, 903, 937, 1837
BslFI	GGGAC	2 cut(s)	18, 594
BslI	CCNNNNNNNGG	6 cut(s)	92, 125, 288, 1705, 1991, 2224
BsmAI	GTCTC	3 cut(s)	353, 982, 1257
BsmFI	GGGAC	2 cut(s)	18, 594
BsmI	GAATGC	1 cut(s)	1868
BsnI	GGCC	5 cut(s)	187, 210, 292, 1995, 2217
Bsp1286I	GDGCHC	2 cut(s)	1206, 1272
Bsp1407I	TGTACA	2 cut(s)	240, 1387
Bsp68I	TCGCGA	1 cut(s)	123
BspACI	CCGC	7 cut(s)	51, 96, 275, 338, 1024, 1924, 1982
BspANI	GGCC	5 cut(s)	187, 210, 292, 1995, 2217
BspCNI	CTCAG	4 cut(s)	538, 820, 1726, 1803
BspFNI	CGCG	3 cut(s)	123, 338, 1026
BspLI	GGNNCC	7 cut(s)	279, 610, 901, 1070, 1627, 2140, 2241
BspMAI	CTGCAG	2 cut(s)	649, 931
BspPI	GGATC	7 cut(s)	110, 677, 1310, 2133, 2146, 2234, 2247
BspQI	GCTCTTC	1 cut(s)	830
BspT107I	GGYRCC	2 cut(s)	899, 1625
BspTI	CTTAAG	2 cut(s)	473, 509
BsrDI	GCAATG	4 cut(s)	270, 503, 1602, 2106
BsrGI	TGTACA	2 cut(s)	240, 1387
BsrI	ACTGG	5 cut(s)	30, 612, 642, 1355, 2197
BssECI	CCNNGG	4 cut(s)	54, 1072, 2043, 2235
BssNI	GRCGYC	1 cut(s)	1780
BssSI	CACGAG	2 cut(s)	305, 555
BssT1I	CCWWGG	3 cut(s)	54, 2043, 2235
Bst2BI	CACGAG	2 cut(s)	305, 555
Bst4CI	ACNGT	7 cut(s)	11, 128, 393, 437, 775, 1392, 2031
Bst6I	CTCTTC	1 cut(s)	830
BstACI	GRCGYC	1 cut(s)	1780
BstAFI	CTTAAG	2 cut(s)	473, 509
BstAPI	GCANNNNNTGC	1 cut(s)	971
BstAUI	TGTACA	2 cut(s)	240, 1387
BstBAI	YACGTR	1 cut(s)	70
BstC8I	GCNNGC	8 cut(s)	199, 290, 719, 1262, 1470, 1507, 1976, 2125
BstDEI	CTNAG	8 cut(s)	546, 807, 1248, 1704, 1734, 1811, 1818, 1920
BstDSI	CCRYGG	1 cut(s)	1072
BstENI	CCTNNNNNAGG	2 cut(s)	1703, 1989
BstF5I	GGATG	3 cut(s)	1300, 1845, 2175
BstFNI	CGCG	3 cut(s)	123, 338, 1026
BstHHI	GCGC	4 cut(s)	203, 1397, 1582, 1792
BstMAI	GTCTC	3 cut(s)	353, 982, 1257
BstMWI	GCNNNNNNNGC	4 cut(s)	207, 638, 647, 971
BstNSI	RCATGY	2 cut(s)	1509, 1769
BstSCI	CCNGG	1 cut(s)	935
BstSFI	CTRYAG	2 cut(s)	645, 927
BstSLI	GKGCMC	1 cut(s)	1206
BstUI	CGCG	3 cut(s)	123, 338, 1026
BstV1I	GCAGC	7 cut(s)	631, 653, 659, 938, 941, 1804, 1939
BstV2I	GAAGAC	2 cut(s)	81, 506
BstX2I	RGATCY	4 cut(s)	872, 1755, 2138, 2239
BstYI	RGATCY	4 cut(s)	872, 1755, 2138, 2239
Bsu36I	CCTNAGG	2 cut(s)	807, 1734
BsuRI	GGCC	5 cut(s)	187, 210, 292, 1995, 2217
BtgI	CCRYGG	1 cut(s)	1072
BtgZI	GCGATG	1 cut(s)	1041
BtsCI	GGATG	3 cut(s)	1300, 1845, 2175
BtsI	GCAGTG	2 cut(s)	1255, 1272
BtsIMutI	CAGTG	7 cut(s)	619, 995, 1255, 1272, 1397, 2140, 2190
BtuMI	TCGCGA	1 cut(s)	123
Cac8I	GCNNGC	8 cut(s)	199, 290, 719, 1262, 1470, 1507, 1976, 2125
CaiI	CAGNNNCTG	1 cut(s)	1997
CfoI	GCGC	4 cut(s)	203, 1397, 1582, 1792
Cfr13I	GGNCC	4 cut(s)	608, 1328, 1834, 1994
CseI	GACGC	1 cut(s)	1784
Csp6I	GTAC	6 cut(s)	241, 310, 334, 1388, 1861, 1909
CviQI	GTAC	6 cut(s)	241, 310, 334, 1388, 1861, 1909
DdeI	CTNAG	8 cut(s)	546, 807, 1248, 1704, 1734, 1811, 1818, 1920
DraI	TTTAAA	2 cut(s)	1669, 2266
DriI	GACNNNNNGTC	1 cut(s)	2029
EaeI	YGGCCR	1 cut(s)	208
Eam1104I	CTCTTC	1 cut(s)	830
Eam1105I	GACNNNNNGTC	1 cut(s)	2029
EarI	CTCTTC	1 cut(s)	830
EciI	GGCGGA	1 cut(s)	40
Eco130I	CCWWGG	3 cut(s)	54, 2043, 2235
Eco24I	GRGCYC	1 cut(s)	1272
Eco47I	GGWCC	3 cut(s)	608, 1328, 1834
Eco57I	CTGAAG	4 cut(s)	672, 745, 1419, 1814
Eco72I	CACGTG	1 cut(s)	70
Eco81I	CCTNAGG	2 cut(s)	807, 1734
EcoNI	CCTNNNNNAGG	2 cut(s)	1703, 1989
EcoO109I	RGGNCCY	1 cut(s)	1994
EcoT14I	CCWWGG	3 cut(s)	54, 2043, 2235
EcoT22I	ATGCAT	2 cut(s)	673, 1870
EcoT38I	GRGCYC	1 cut(s)	1272
ErhI	CCWWGG	3 cut(s)	54, 2043, 2235
FaqI	GGGAC	2 cut(s)	18, 594
FauI	CCCGC	1 cut(s)	1989
FbaI	TGATCA	3 cut(s)	985, 1030, 1942
FblI	GTMKAC	2 cut(s)	108, 1825
FokI	GGATG	3 cut(s)	1307, 1852, 2182
FriOI	GRGCYC	1 cut(s)	1272
FspBI	CTAG	5 cut(s)	528, 788, 914, 1658, 2087
GlaI	GCGC	4 cut(s)	202, 1396, 1581, 1791
GsaI	CCCAGC	1 cut(s)	1086
HaeIII	GGCC	5 cut(s)	187, 210, 292, 1995, 2217
HapII	CCGG	4 cut(s)	211, 903, 937, 1837
HgaI	GACGC	1 cut(s)	1784
HhaI	GCGC	4 cut(s)	203, 1397, 1582, 1792
Hin1I	GRCGYC	1 cut(s)	1780
Hin6I	GCGC	4 cut(s)	201, 1395, 1580, 1790
HinP1I	GCGC	4 cut(s)	201, 1395, 1580, 1790
HincII	GTYRAC	3 cut(s)	109, 778, 1017
HindII	GTYRAC	3 cut(s)	109, 778, 1017
HindIII	AAGCTT	4 cut(s)	249, 1262, 1662, 1972
HinfI	GANTC	4 cut(s)	296, 1139, 1486, 1988
HpaI	GTTAAC	1 cut(s)	778
HpaII	CCGG	4 cut(s)	211, 903, 937, 1837
HphI	GGTGA	5 cut(s)	23, 140, 605, 1724, 2072
Hpy166II	GTNNAC	7 cut(s)	109, 778, 1017, 1204, 1687, 1826, 2176
Hpy8I	GTNNAC	7 cut(s)	109, 778, 1017, 1204, 1687, 1826, 2176
Hpy99I	CGWCG	3 cut(s)	83, 113, 128
HpyAV	CCTTC	5 cut(s)	125, 238, 696, 1225, 2123
HpyCH4III	ACNGT	7 cut(s)	11, 128, 393, 437, 775, 1392, 2031
HpyCH4IV	ACGT	7 cut(s)	69, 712, 1206, 1522, 1540, 1683, 1780
HpyF10VI	GCNNNNNNNGC	4 cut(s)	207, 638, 647, 971
HpyF3I	CTNAG	8 cut(s)	546, 807, 1248, 1704, 1734, 1811, 1818, 1920
HpySE526I	ACGT	7 cut(s)	69, 712, 1206, 1522, 1540, 1683, 1780
Hsp92I	GRCGYC	1 cut(s)	1780
HspAI	GCGC	4 cut(s)	201, 1395, 1580, 1790
Ksp22I	TGATCA	3 cut(s)	985, 1030, 1942
KspAI	GTTAAC	1 cut(s)	778
LguI	GCTCTTC	1 cut(s)	830
LmnI	GCTCC	4 cut(s)	411, 560, 602, 2080
Lsp1109I	GCAGC	7 cut(s)	631, 653, 659, 938, 941, 1804, 1939
LweI	GCATC	3 cut(s)	291, 2056, 2086
MaeI	CTAG	5 cut(s)	528, 788, 914, 1658, 2087
MaeII	ACGT	7 cut(s)	69, 712, 1206, 1522, 1540, 1683, 1780
MaeIII	GTNAC	4 cut(s)	1093, 1679, 1761, 2031
MflI	RGATCY	4 cut(s)	872, 1755, 2138, 2239
MhlI	GDGCHC	2 cut(s)	1206, 1272
MlyI	GAGTC	1 cut(s)	305
MmeI	TCCRAC	2 cut(s)	726, 1335
Mph1103I	ATGCAT	2 cut(s)	673, 1870
MseI	TTAA	8 cut(s)	474, 510, 540, 705, 777, 1668, 2019, 2265
MslI	CAYNNNNRTG	3 cut(s)	884, 1283, 2019
MspA1I	CMGCKG	2 cut(s)	644, 650
MspCI	CTTAAG	2 cut(s)	473, 509
MspI	CCGG	4 cut(s)	211, 903, 937, 1837
MspR9I	CCNGG	1 cut(s)	937
Mva1269I	GAATGC	1 cut(s)	1868
MvnI	CGCG	3 cut(s)	123, 338, 1026
MwoI	GCNNNNNNNGC	4 cut(s)	207, 638, 647, 971
NciI	CCSGG	1 cut(s)	937
NlaIV	GGNNCC	7 cut(s)	279, 610, 901, 1070, 1627, 2140, 2241
NmuCI	GTSAC	3 cut(s)	1679, 1761, 2031
NruI	TCGCGA	1 cut(s)	123
NsiI	ATGCAT	2 cut(s)	673, 1870
NspI	RCATGY	2 cut(s)	1509, 1769
OliI	CACNNNNGTG	1 cut(s)	1283
PaeI	GCATGC	1 cut(s)	1509
PciSI	GCTCTTC	1 cut(s)	830
PcsI	WCGNNNNNNNCGW	2 cut(s)	75, 1203
PctI	GAATGC	1 cut(s)	1868
PfeI	GAWTC	3 cut(s)	1139, 1486, 1988
PflFI	GACNNNGTC	1 cut(s)	518
PfoI	TCCNGGA	1 cut(s)	935
PleI	GAGTC	1 cut(s)	304
PmaCI	CACGTG	1 cut(s)	70
PmlI	CACGTG	1 cut(s)	70
PpsI	GAGTC	1 cut(s)	304
Ppu21I	YACGTR	1 cut(s)	70
Psp1406I	AACGTT	1 cut(s)	712
PspCI	CACGTG	1 cut(s)	70
PspFI	CCCAGC	1 cut(s)	1082
PspN4I	GGNNCC	7 cut(s)	279, 610, 901, 1070, 1627, 2140, 2241
PspPI	GGNCC	4 cut(s)	608, 1328, 1834, 1994
PstI	CTGCAG	2 cut(s)	649, 931
PstNI	CAGNNNCTG	1 cut(s)	1997
PsuI	RGATCY	4 cut(s)	872, 1755, 2138, 2239
PsyI	GACNNNGTC	1 cut(s)	518
PvuII	CAGCTG	2 cut(s)	644, 650
RruI	TCGCGA	1 cut(s)	123
RsaI	GTAC	6 cut(s)	242, 311, 335, 1389, 1862, 1910
RsaNI	GTAC	6 cut(s)	241, 310, 334, 1388, 1861, 1909
RseI	CAYNNNNRTG	3 cut(s)	884, 1283, 2019
SalI	GTCGAC	1 cut(s)	107
SapI	GCTCTTC	1 cut(s)	830
SaqAI	TTAA	8 cut(s)	474, 510, 540, 705, 777, 1668, 2019, 2265
Sau96I	GGNCC	4 cut(s)	608, 1328, 1834, 1994
SchI	GAGTC	1 cut(s)	305
ScrFI	CCNGG	1 cut(s)	937
SduI	GDGCHC	2 cut(s)	1206, 1272
SfaNI	GCATC	3 cut(s)	291, 2056, 2086
SfcI	CTRYAG	2 cut(s)	645, 927
SinI	GGWCC	3 cut(s)	608, 1328, 1834
SmiMI	CAYNNNNRTG	3 cut(s)	884, 1283, 2019
SmlI	CTYRAG	4 cut(s)	356, 473, 509, 841
SmoI	CTYRAG	4 cut(s)	356, 473, 509, 841
SphI	GCATGC	1 cut(s)	1509
SsiI	CCGC	7 cut(s)	51, 96, 275, 338, 1024, 1924, 1982
SspI	AATATT	1 cut(s)	1439
SspMI	CTAG	5 cut(s)	528, 788, 914, 1658, 2087
StyD4I	CCNGG	1 cut(s)	935
StyI	CCWWGG	3 cut(s)	54, 2043, 2235
TaaI	ACNGT	7 cut(s)	11, 128, 393, 437, 775, 1392, 2031
TaiI	ACGT	7 cut(s)	72, 715, 1209, 1525, 1543, 1686, 1783
TaqI	TCGA	3 cut(s)	108, 222, 299
TaqII	GACCGA	1 cut(s)	341
TatI	WGTACW	5 cut(s)	240, 309, 1387, 1860, 1908
TauI	GCSGC	3 cut(s)	99, 341, 1927
TfiI	GAWTC	3 cut(s)	1139, 1486, 1988
Tru1I	TTAA	8 cut(s)	474, 510, 540, 705, 777, 1668, 2019, 2265
Tru9I	TTAA	8 cut(s)	474, 510, 540, 705, 777, 1668, 2019, 2265
TscAI	CASTG	7 cut(s)	619, 995, 1262, 1279, 1397, 2140, 2197
TseFI	GTSAC	3 cut(s)	1679, 1761, 2031
TseI	GCWGC	7 cut(s)	641, 644, 647, 929, 951, 1792, 1927
Tsp45I	GTSAC	3 cut(s)	1679, 1761, 2031
TspDTI	ATGAA	6 cut(s)	642, 1356, 1499, 1545, 1613, 2105
TspGWI	ACGGA	1 cut(s)	1061
TspRI	CASTG	7 cut(s)	619, 995, 1262, 1279, 1397, 2140, 2197
Tth111I	GACNNNGTC	1 cut(s)	518
Vha464I	CTTAAG	2 cut(s)	473, 509
VneI	GTGCAC	1 cut(s)	1202
VpaK11BI	GGWCC	3 cut(s)	608, 1328, 1834
XagI	CCTNNNNNAGG	2 cut(s)	1703, 1989
XapI	RAATTY	4 cut(s)	44, 425, 1035, 2204
XbaI	TCTAGA	2 cut(s)	527, 787
XceI	RCATGY	2 cut(s)	1509, 1769
XmiI	GTMKAC	2 cut(s)	108, 1825
XspI	CTAG	5 cut(s)	528, 788, 914, 1658, 2087
ZraI	GACGTC	1 cut(s)	1781
Zsp2I	ATGCAT	2 cut(s)	673, 1870

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.