Rroxscaffold_4G00312270

DNA repair helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001033
Physical Location & Seq
Reverse (-)
35116795 .. 35122931
6137 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00312270.1

Sequence Viewer

Length: 2283 bp
ATGGGACACGGTGACAAAGGTCGACCCAGTAAAAAGCTCCGACTCTCTATCAAGGAAAAATCAAATGTGCAAAACGACGACGTACACTACTGCAGCGGTGAGGAAGAAGAACATGTCCAGCATGGTGGTGAAGGGAAATACAGAGATTTTACCAAACTAGAATTGAAGCCTGACCACCCTAACCGCCCTATATGGGCTTGCCCTGATGGCCGAATATTCCTCGAATCTTTCTCCGCTTTGTACAAACAAGCCTATGATTTCCTCATTGCCATTGCCGAACCTGTTTGCAGGCAAGAGTTGATGCACGAGTATAACTTGACCCCGCATTCGCTGTATGCGGCTGTGTCAGTTGGTCTTGCTACGGAGACCATTATTTCTGTGCTTAATAAGCTGTCAAAGGTCAACCTGCCGAGAGAAGTTATTGCTTTTATACACGCTTCTACTGCGAATTATGGCAAACTGAAGCTCGTGCTTAAGAAGAATCGCTACTTTGTATTGGAAATATTGCTTAAGGACGACTTTATATCTCAATCAAGGATTGCTTCAGAGGGTTCTCATGGAACTGGTGGTTTCACAATTAGCACAACAATGGGTGAGATTGGAGCTGGTCCAAGTGATTTGCTAAATGAAGCAGAATTGGCAGCTGCCGCTGAAGAAAAAGAAACTCATGCATTTGAAATTGATCCTTCAAAGGTTGAGAATGTTAAGCAGCGTTGCTTGCCAGAATCTTTGAACTATCCCATGTTGGAGGAGTATGACTTCAGAAATGATACAGTTAACCCTGATCTTGACATGGAACTAAAGCCTCAAGCGCAACCACGACCATATCAGGAGAAGAGCTTGAGTAAAATGTTTGGGAACGGGAGAGCAAGATCTGGCATAATTGTACTGCCTTGTGGTGCCGGAAAGTCTCTTGTTGGTGTTTCTGCTGCCTCCAGGATAAGAAAGAGCTGCCTTTGTTTAGCAACAAATGCTGTTTCTGTTGATCAGTGGGCTTTTCAGTTTAAGTTGTGGTCAACCATCCGAGATGAACAAATTTGTCGTTTCACATCTGATAGTAAAGAAAGAATCCATGGAAATGCTGGGGTAGTTGTGACAACATATAACATGATTGGTTTTGGTGGCAAGAGGTCTGAAGAATCTGAAAAGATCATTGAAGAAATAAGAAACAGAGAATGGGGATTGCTTCTCATGGATGAGGTGCACGTCGTTCCTGCTGAAATGTTTCGAAATGTCATCAGGCTTACTAAATCTCACTGCAAGCTTGGTCTCACCGCCACACTTGTGAGAGAGGATGAGAAGATCACAGACCTTAACTTCCTTATTGGTCCAAAGTTGTATGAAGCAAACTGGTTGGATTTGGTTAAAGGTGGATTTATTGCAAACGTACAGTGTGCTGAAGTATGGTGTCCAATGACAAAAGAGTTTTTTGTGGAATATTTGAAGAAAGAAAACTCAAAGAAAAAGCAGGCACTTTATGTCATGAATCCAAATAAGTTTAGGGCATGCGAGTTTCTTATAAGGTTTCATGAAGAGCAACGTGGTGATAAAATTATTGTGTTTGCTGACAATCTCTTTGCCCTCGCGGAGTATGCAAAGAAACTTCGGAAACCTATGATCTGTGGTAGTACCAGCCATGTGGAGAGGACGAAAATTCTAGAAGCCTTCAAAACAAGTCAGAACATAAATACTGTTTTCTTGTCTAAGGTGGGTGATAATTCCATAGACATTCCTGAGGCAAATGTGATTATTCAGATTTCATCACATGCTGGTTCAAGACGTCAAGAAGCACAGCGTCTTGGTCGTATTTTAAGAGCTAAGGGTAGACCTCAGGATCGGATAGCAGGAGGAAAAGAGGAGTACAATGCATTTTTTTATTCCCTCGTGTCTACGGATACTCAGGAAATGTACTTTTCAACTAAACGGCAGCAGTTTTTGATTGATCAAGGTTATAGTTTTAAGGTTATTACAAGCTTGCCTCCTCCTGAATCAGGAGCTGATTTGAGCTATCATCGTGTTGAAGACCAACTGGCACTACTCTCAAAGGTGGTGAGTGCTGGTGATGATAAAGTTGGTTTAGAGCCACATGACGATGATGCAGATGATATTTCCCTTCAGAAAGCACGTCGTTCAACAGGATCCATGAGTGAGATGTCGGGTTCAGATGGGATGGTTTACATGGAAGATAGTAATGAAAAACACAAGCACAGCAAAGTACAGGTGAAGAGTAAGCCAAGGGATCCGGCAAAGAGACATCATTTGTTTAAACAACGCCGTGGCTGA

Protein Analysis

760

Amino Acids

85.76

Weight (kDa)

8.06

Isoelectric Point (pI)

37.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helicase_C_3 PF13625 64 - 165 8.1e-27 Helicase conserved C-terminal domain
ResIII PF04851 272 - 429 7.3e-18 Type III restriction enzyme, res subunit
ERCC3_RAD25_C PF16203 454 - 700 2.1e-115 ERCC3/RAD25/XPB C-terminal helicase
Helicase_C PF00271 504 - 606 9.1e-13 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1520
AatII GACGTC 1 cut(s) 1783
Acc36I ACCTGC 1 cut(s) 414
AccB1I GGYRCC 1 cut(s) 899
AccI GTMKAC 3 cut(s) 22, 1825, 1889
AccII CGCG 1 cut(s) 1586
AciI CCGC 8 cut(s) 96, 184, 234, 323, 338, 648, 1275, 1586
AclWI GGATC 6 cut(s) 677, 1842, 2133, 2146, 2234, 2247
AcoI YGGCCR 1 cut(s) 208
AcsI RAATTY 2 cut(s) 1035, 1653
AcuI CTGAAG 7 cut(s) 482, 528, 672, 745, 1155, 1419, 2099
AcyI GRCGYC 1 cut(s) 1780
AfaI GTAC 8 cut(s) 84, 242, 888, 1389, 1630, 1862, 1910, 2217
AfiI CCNNNNNNNGG 2 cut(s) 193, 1991
AflII CTTAAG 2 cut(s) 473, 509
AflIII ACRYGT 1 cut(s) 112
AjiI CACGTC 2 cut(s) 1207, 2126
AjnI CCWGG 1 cut(s) 935
AleI CACNNNNGTG 1 cut(s) 1283
Alw21I GWGCWC 1 cut(s) 1206
Alw26I GTCTC 4 cut(s) 359, 915, 1274, 2245
Alw44I GTGCAC 1 cut(s) 1202
AlwI GGATC 6 cut(s) 677, 1842, 2133, 2146, 2234, 2247
AlwNI CAGNNNCTG 1 cut(s) 1997
AoxI GGCC 1 cut(s) 208
ApaLI GTGCAC 1 cut(s) 1202
ApeKI GCWGC 7 cut(s) 93, 641, 644, 709, 929, 951, 1927
ApoI RAATTY 2 cut(s) 1035, 1653
ArsI GACNNNNNNTTYG 4 cut(s) 310, 342, 1559, 1591
Asp700I GAANNNNTTC 1 cut(s) 1224
AspLEI GCGC 1 cut(s) 814
AspS9I GGNCC 2 cut(s) 608, 1328
AsuII TTCGAA 1 cut(s) 1228
AvaII GGWCC 2 cut(s) 608, 1328
AxyI CCTNAGG 2 cut(s) 1734, 1830
BaeGI GKGCMC 1 cut(s) 1206
BamHI GGATCC 2 cut(s) 2138, 2239
BanI GGYRCC 1 cut(s) 899
BarI GAAGNNNNNNTAC 2 cut(s) 470, 502
BauI CACGAG 3 cut(s) 305, 467, 1883
BbsI GAAGAC 1 cut(s) 2028
Bbv12I GWGCWC 1 cut(s) 1206
BbvI GCAGC 7 cut(s) 105, 631, 653, 721, 916, 938, 1939
BccI CCATC 4 cut(s) 200, 1028, 2159, 2164
BceAI ACGGC 2 cut(s) 1940, 2259
BciT130I CCWGG 1 cut(s) 937
BciVI GTATCC 1 cut(s) 1888
BclI TGATCA 2 cut(s) 985, 1942
BcoDI GTCTC 4 cut(s) 359, 915, 1274, 2245
BfaI CTAG 2 cut(s) 158, 1658
BfmI CTRYAG 1 cut(s) 91
BfrI CTTAAG 2 cut(s) 473, 509
BfuAI ACCTGC 1 cut(s) 414
BfuI GTATCC 1 cut(s) 1888
BglI GCCNNNNNGGC 1 cut(s) 207
BglII AGATCT 1 cut(s) 872
BisI GCNGC 9 cut(s) 94, 339, 642, 645, 648, 710, 930, 952, 1928
BlsI GCNGC 9 cut(s) 95, 340, 643, 646, 649, 711, 931, 953, 1929
Bme1390I CCNGG 1 cut(s) 937
Bme18I GGWCC 2 cut(s) 608, 1328
BmgBI CACGTC 2 cut(s) 1207, 2126
BmgT120I GGNCC 2 cut(s) 608, 1328
BmiI GGNNCC 3 cut(s) 901, 2140, 2241
BmrFI CCNGG 1 cut(s) 937
BmrI ACTGGG 1 cut(s) 21
BmsI GCATC 2 cut(s) 291, 2086
BmuI ACTGGG 1 cut(s) 21
BoxI GACNNNNGTC 1 cut(s) 18
BpiI GAAGAC 1 cut(s) 2028
BpmI CTGGAG 1 cut(s) 919
Bpu10I CCTNAGC 1 cut(s) 1818
Bpu14I TTCGAA 1 cut(s) 1228
BpuEI CTTGAG 2 cut(s) 792, 862
BsaHI GRCGYC 1 cut(s) 1780
BsaI GGTCTC 2 cut(s) 359, 1274
BsaJI CCNNGG 3 cut(s) 1072, 2234, 2275
Bsc4I CCNNNNNNNGG 2 cut(s) 193, 1991
Bse1I ACTGG 4 cut(s) 27, 568, 1355, 2034
Bse21I CCTNAGG 2 cut(s) 1734, 1830
Bse3DI GCAATG 2 cut(s) 264, 270
BseBI CCWGG 1 cut(s) 937
BseDI CCNNGG 3 cut(s) 1072, 2234, 2275
BseGI GGATG 4 cut(s) 1020, 1201, 1300, 2175
BseLI CCNNNNNNNGG 2 cut(s) 193, 1991
BseMI GCAATG 2 cut(s) 264, 270
BseMII CTCAG 3 cut(s) 1725, 1844, 1913
BseNI ACTGG 4 cut(s) 27, 568, 1355, 2034
BseRI GAGGAG 3 cut(s) 764, 1871, 1971
BseSI GKGCMC 1 cut(s) 1206
BseXI GCAGC 7 cut(s) 105, 631, 653, 721, 916, 938, 1939
BseYI CCCAGC 1 cut(s) 1082
Bsh1236I CGCG 1 cut(s) 1586
BshFI GGCC 1 cut(s) 210
BshNI GGYRCC 1 cut(s) 899
BsiHKAI GWGCWC 1 cut(s) 1206
BsiSI CCGG 2 cut(s) 903, 2243
BslFI GGGAC 1 cut(s) 18
BslI CCNNNNNNNGG 2 cut(s) 193, 1991
BsmAI GTCTC 4 cut(s) 359, 915, 1274, 2245
BsmFI GGGAC 1 cut(s) 18
BsmI GAATGC 1 cut(s) 325
BsnI GGCC 1 cut(s) 210
Bso31I GGTCTC 2 cut(s) 359, 1274
Bsp119I TTCGAA 1 cut(s) 1228
Bsp1286I GDGCHC 1 cut(s) 1206
Bsp1407I TGTACA 1 cut(s) 240
Bsp19I CCATGG 1 cut(s) 1072
BspACI CCGC 8 cut(s) 96, 184, 234, 323, 338, 648, 1275, 1586
BspANI GGCC 1 cut(s) 210
BspCNI CTCAG 3 cut(s) 1726, 1843, 1912
BspFNI CGCG 1 cut(s) 1586
BspHI TCATGA 2 cut(s) 1482, 1528
BspLI GGNNCC 3 cut(s) 901, 2140, 2241
BspMAI CTGCAG 1 cut(s) 95
BspMI ACCTGC 1 cut(s) 414
BspPI GGATC 6 cut(s) 677, 1842, 2133, 2146, 2234, 2247
BspQI GCTCTTC 2 cut(s) 830, 1527
BspT104I TTCGAA 1 cut(s) 1228
BspT107I GGYRCC 1 cut(s) 899
BspTI CTTAAG 2 cut(s) 473, 509
BspTNI GGTCTC 2 cut(s) 359, 1274
BsrDI GCAATG 2 cut(s) 264, 270
BsrGI TGTACA 1 cut(s) 240
BsrI ACTGG 4 cut(s) 27, 568, 1355, 2034
BssECI CCNNGG 3 cut(s) 1072, 2234, 2275
BssNI GRCGYC 1 cut(s) 1780
BssSI CACGAG 3 cut(s) 305, 467, 1883
BssT1I CCWWGG 2 cut(s) 1072, 2234
Bst2BI CACGAG 3 cut(s) 305, 467, 1883
Bst2UI CCWGG 1 cut(s) 937
Bst4CI ACNGT 4 cut(s) 11, 775, 1392, 1693
Bst6I CTCTTC 3 cut(s) 830, 1527, 2219
BstACI GRCGYC 1 cut(s) 1780
BstAFI CTTAAG 2 cut(s) 473, 509
BstAPI GCANNNNNTGC 1 cut(s) 971
BstAUI TGTACA 1 cut(s) 240
BstBI TTCGAA 1 cut(s) 1228
BstC8I GCNNGC 7 cut(s) 199, 290, 719, 1262, 1470, 1507, 1976
BstDEI CTNAG 5 cut(s) 1704, 1734, 1818, 1830, 1899
BstDSI CCRYGG 2 cut(s) 1072, 2275
BstENI CCTNNNNNAGG 1 cut(s) 1989
BstF5I GGATG 4 cut(s) 1020, 1201, 1300, 2175
BstFNI CGCG 1 cut(s) 1586
BstHHI GCGC 1 cut(s) 814
BstMAI GTCTC 4 cut(s) 359, 915, 1274, 2245
BstMWI GCNNNNNNNGC 9 cut(s) 207, 388, 443, 638, 647, 718, 811, 971, 1592
BstNI CCWGG 1 cut(s) 937
BstNSI RCATGY 3 cut(s) 116, 1509, 1769
BstPAI GACNNNNGTC 1 cut(s) 18
BstSCI CCNGG 1 cut(s) 935
BstSFI CTRYAG 1 cut(s) 91
BstSLI GKGCMC 1 cut(s) 1206
BstUI CGCG 1 cut(s) 1586
BstV1I GCAGC 7 cut(s) 105, 631, 653, 721, 916, 938, 1939
BstV2I GAAGAC 1 cut(s) 2028
BstX2I RGATCY 3 cut(s) 872, 2138, 2239
BstXI CCANNNNNNTGG 2 cut(s) 125, 1639
BstYI RGATCY 3 cut(s) 872, 2138, 2239
Bsu36I CCTNAGG 2 cut(s) 1734, 1830
BsuI GTATCC 1 cut(s) 1888
BsuRI GGCC 1 cut(s) 210
BtgI CCRYGG 2 cut(s) 1072, 2275
BtrI CACGTC 2 cut(s) 1207, 2126
BtsCI GGATG 4 cut(s) 1020, 1201, 1300, 2175
BtsI GCAGTG 1 cut(s) 1255
BtsIMutI CAGTG 3 cut(s) 995, 1255, 1397
BveI ACCTGC 1 cut(s) 414
Cac8I GCNNGC 7 cut(s) 199, 290, 719, 1262, 1470, 1507, 1976
CaiI CAGNNNCTG 1 cut(s) 1997
CciI TCATGA 2 cut(s) 1482, 1528
CfoI GCGC 1 cut(s) 814
Cfr13I GGNCC 2 cut(s) 608, 1328
CseI GACGC 1 cut(s) 1784
Csp6I GTAC 8 cut(s) 83, 241, 887, 1388, 1629, 1861, 1909, 2216
CviQI GTAC 8 cut(s) 83, 241, 887, 1388, 1629, 1861, 1909, 2216
DdeI CTNAG 5 cut(s) 1704, 1734, 1818, 1830, 1899
DraI TTTAAA 1 cut(s) 2266
EaeI YGGCCR 1 cut(s) 208
Eam1104I CTCTTC 3 cut(s) 830, 1527, 2219
EarI CTCTTC 3 cut(s) 830, 1527, 2219
Eco130I CCWWGG 2 cut(s) 1072, 2234
Eco31I GGTCTC 2 cut(s) 359, 1274
Eco47I GGWCC 2 cut(s) 608, 1328
Eco57I CTGAAG 7 cut(s) 482, 528, 672, 745, 1155, 1419, 2099
Eco81I CCTNAGG 2 cut(s) 1734, 1830
EcoNI CCTNNNNNAGG 1 cut(s) 1989
EcoRII CCWGG 1 cut(s) 935
EcoT14I CCWWGG 2 cut(s) 1072, 2234
EcoT22I ATGCAT 2 cut(s) 673, 1870
ErhI CCWWGG 2 cut(s) 1072, 2234
FalI AAGNNNNNCTT 4 cut(s) 503, 535, 526, 558
FaqI GGGAC 1 cut(s) 18
FauI CCCGC 1 cut(s) 330
FbaI TGATCA 2 cut(s) 985, 1942
FblI GTMKAC 3 cut(s) 22, 1825, 1889
Fnu4HI GCNGC 9 cut(s) 94, 339, 642, 645, 648, 710, 930, 952, 1928
FokI GGATG 4 cut(s) 1007, 1208, 1307, 2182
Fsp4HI GCNGC 9 cut(s) 94, 339, 642, 645, 648, 710, 930, 952, 1928
FspBI CTAG 2 cut(s) 158, 1658
GlaI GCGC 1 cut(s) 813
GluI GCNGC 9 cut(s) 94, 339, 642, 645, 648, 710, 930, 952, 1928
GsaI CCCAGC 1 cut(s) 1086
GsuI CTGGAG 1 cut(s) 919
HaeIII GGCC 1 cut(s) 210
HapII CCGG 2 cut(s) 903, 2243
HgaI GACGC 1 cut(s) 1784
HhaI GCGC 1 cut(s) 814
Hin1I GRCGYC 1 cut(s) 1780
Hin6I GCGC 1 cut(s) 812
HinP1I GCGC 1 cut(s) 812
HincII GTYRAC 4 cut(s) 23, 403, 778, 1017
HindII GTYRAC 4 cut(s) 23, 403, 778, 1017
HindIII AAGCTT 2 cut(s) 1262, 1972
HinfI GANTC 8 cut(s) 42, 224, 481, 725, 1068, 1139, 1486, 1988
HpaI GTTAAC 1 cut(s) 778
HpaII CCGG 2 cut(s) 903, 2243
Hpy166II GTNNAC 9 cut(s) 23, 85, 403, 778, 1017, 1204, 1826, 1890, 2176
Hpy8I GTNNAC 9 cut(s) 23, 85, 403, 778, 1017, 1204, 1826, 1890, 2176
Hpy99I CGWCG 4 cut(s) 80, 83, 1211, 2130
HpyAV CCTTC 4 cut(s) 125, 696, 1675, 2123
HpyCH4III ACNGT 4 cut(s) 11, 775, 1392, 1693
HpyCH4IV ACGT 6 cut(s) 81, 1206, 1386, 1540, 1780, 2125
HpyF10VI GCNNNNNNNGC 9 cut(s) 207, 388, 443, 638, 647, 718, 811, 971, 1592
HpyF3I CTNAG 5 cut(s) 1704, 1734, 1818, 1830, 1899
HpySE526I ACGT 6 cut(s) 81, 1206, 1386, 1540, 1780, 2125
Hsp92I GRCGYC 1 cut(s) 1780
HspAI GCGC 1 cut(s) 812
Ksp22I TGATCA 2 cut(s) 985, 1942
KspAI GTTAAC 1 cut(s) 778
LguI GCTCTTC 2 cut(s) 830, 1527
LmnI GCTCC 3 cut(s) 42, 602, 1994
Lsp1109I GCAGC 7 cut(s) 105, 631, 653, 721, 916, 938, 1939
LweI GCATC 2 cut(s) 291, 2086
MaeI CTAG 2 cut(s) 158, 1658
MaeII ACGT 6 cut(s) 81, 1206, 1386, 1540, 1780, 2125
MaeIII GTNAC 2 cut(s) 11, 1093
MflI RGATCY 3 cut(s) 872, 2138, 2239
MhlI GDGCHC 1 cut(s) 1206
MlyI GAGTC 1 cut(s) 36
MmeI TCCRAC 3 cut(s) 64, 726, 1335
Mph1103I ATGCAT 2 cut(s) 673, 1870
MroXI GAANNNNTTC 1 cut(s) 1224
MslI CAYNNNNRTG 5 cut(s) 126, 587, 1077, 1283, 2091
MspA1I CMGCKG 3 cut(s) 96, 644, 650
MspCI CTTAAG 2 cut(s) 473, 509
MspI CCGG 2 cut(s) 903, 2243
MspR9I CCNGG 1 cut(s) 937
MssI GTTTAAAC 1 cut(s) 2266
Mva1269I GAATGC 1 cut(s) 325
MvaI CCWGG 1 cut(s) 937
MvnI CGCG 1 cut(s) 1586
MwoI GCNNNNNNNGC 9 cut(s) 207, 388, 443, 638, 647, 718, 811, 971, 1592
NcoI CCATGG 1 cut(s) 1072
NlaIV GGNNCC 3 cut(s) 901, 2140, 2241
NmeAIII GCCGAG 1 cut(s) 435
NmuCI GTSAC 2 cut(s) 11, 1093
NsiI ATGCAT 2 cut(s) 673, 1870
NspI RCATGY 3 cut(s) 116, 1509, 1769
NspV TTCGAA 1 cut(s) 1228
OliI CACNNNNGTG 1 cut(s) 1283
PaeI GCATGC 1 cut(s) 1509
PagI TCATGA 2 cut(s) 1482, 1528
PciI ACATGT 1 cut(s) 112
PciSI GCTCTTC 2 cut(s) 830, 1527
PctI GAATGC 1 cut(s) 325
PdmI GAANNNNTTC 1 cut(s) 1224
PfeI GAWTC 7 cut(s) 224, 481, 725, 1068, 1139, 1486, 1988
PfoI TCCNGGA 1 cut(s) 935
PkrI GCNGC 9 cut(s) 95, 340, 643, 646, 649, 711, 931, 953, 1929
PleI GAGTC 1 cut(s) 36
PmeI GTTTAAAC 1 cut(s) 2266
PpsI GAGTC 1 cut(s) 36
PscI ACATGT 1 cut(s) 112
PshAI GACNNNNGTC 1 cut(s) 18
PsiI TTATAA 1 cut(s) 1520
Psp6I CCWGG 1 cut(s) 935
PspFI CCCAGC 1 cut(s) 1082
PspGI CCWGG 1 cut(s) 935
PspN4I GGNNCC 3 cut(s) 901, 2140, 2241
PspPI GGNCC 2 cut(s) 608, 1328
PstI CTGCAG 1 cut(s) 95
PstNI CAGNNNCTG 1 cut(s) 1997
PsuI RGATCY 3 cut(s) 872, 2138, 2239
PvuII CAGCTG 1 cut(s) 644
RsaI GTAC 8 cut(s) 84, 242, 888, 1389, 1630, 1862, 1910, 2217
RsaNI GTAC 8 cut(s) 83, 241, 887, 1388, 1629, 1861, 1909, 2216
RseI CAYNNNNRTG 5 cut(s) 126, 587, 1077, 1283, 2091
SalI GTCGAC 1 cut(s) 21
SapI GCTCTTC 2 cut(s) 830, 1527
SatI GCNGC 9 cut(s) 94, 339, 642, 645, 648, 710, 930, 952, 1928
Sau96I GGNCC 2 cut(s) 608, 1328
SchI GAGTC 1 cut(s) 36
ScrFI CCNGG 1 cut(s) 937
SduI GDGCHC 1 cut(s) 1206
SfaNI GCATC 2 cut(s) 291, 2086
SfcI CTRYAG 1 cut(s) 91
SfuI TTCGAA 1 cut(s) 1228
SinI GGWCC 2 cut(s) 608, 1328
SmiMI CAYNNNNRTG 5 cut(s) 126, 587, 1077, 1283, 2091
SmlI CTYRAG 4 cut(s) 473, 509, 807, 841
SmoI CTYRAG 4 cut(s) 473, 509, 807, 841
SphI GCATGC 1 cut(s) 1509
SsiI CCGC 8 cut(s) 96, 184, 234, 323, 338, 648, 1275, 1586
SspI AATATT 3 cut(s) 216, 504, 1439
SspMI CTAG 2 cut(s) 158, 1658
StyD4I CCNGG 1 cut(s) 935
StyI CCWWGG 2 cut(s) 1072, 2234
TaaI ACNGT 4 cut(s) 11, 775, 1392, 1693
TaiI ACGT 6 cut(s) 84, 1209, 1389, 1543, 1783, 2128
TaqI TCGA 3 cut(s) 22, 222, 1228
TatI WGTACW 5 cut(s) 240, 886, 1860, 1908, 2215
TauI GCSGC 2 cut(s) 341, 650
TfiI GAWTC 7 cut(s) 224, 481, 725, 1068, 1139, 1486, 1988
TscAI CASTG 3 cut(s) 995, 1262, 1397
TseFI GTSAC 2 cut(s) 11, 1093
TseI GCWGC 7 cut(s) 93, 641, 644, 709, 929, 951, 1927
Tsp45I GTSAC 2 cut(s) 11, 1093
TspDTI ATGAA 8 cut(s) 642, 1044, 1356, 1499, 1517, 1545, 1749, 2208
TspGWI ACGGA 2 cut(s) 377, 1907
TspRI CASTG 3 cut(s) 995, 1262, 1397
Vha464I CTTAAG 2 cut(s) 473, 509
VneI GTGCAC 1 cut(s) 1202
VpaK11BI GGWCC 2 cut(s) 608, 1328
XagI CCTNNNNNAGG 1 cut(s) 1989
XapI RAATTY 2 cut(s) 1035, 1653
XbaI TCTAGA 1 cut(s) 1657
XceI RCATGY 3 cut(s) 116, 1509, 1769
XcmI CCANNNNNNNNNTGG 1 cut(s) 1079
XmiI GTMKAC 3 cut(s) 22, 1825, 1889
XmnI GAANNNNTTC 1 cut(s) 1224
XspI CTAG 2 cut(s) 158, 1658
ZraI GACGTC 1 cut(s) 1781
Zsp2I ATGCAT 2 cut(s) 673, 1870
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.