Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr1A

Orthogroup ID

OG0001033

Physical Location & Seq

Genomic Coordinates Forward (+)

32634494 .. 32640640

Gene Structure 6147 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh1AG170500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2283 bp

ATGGGACACGGTGACAAAGGCCGACCCAATAAGAAGCTCAGACTCTCTATCAAGGAAAAATCAAATGTGCAAAACGACGACGTACACTACTGCAGCGGTGAGGAAGAAGACAATGTCCCCCATGGTGGTGAAGGGAAATACAGAGATTTTACCAAACTAGAATTGAAGCCTGACCACCCTAACCGCCCTATATGGGCTTGCCCTGATGGTCGAATATTCCTCGAATCTTTCTCTGCTGTGTACAAACAAGCTTACGATTTCCTCATTGCCATTGCCGAACCTGTTTGCAGGCAAGAGTTGATGCACGAGTATAACTTGACCCCGCATTCGCTGTATGCGGCTGTGTCAGTTGGTCTTGCTACGGAGACCATTATTTCTGTGCTTAATAAGCTGTCAAAGGTCAACCTGCCGAGAGAAGTTATTGATTTCATACACGCTTCTACTGCTAATTATGGCAAACTGAAGCTCGTGCTTAAGAAGAATCGCTACTTTGTGGAATCCCCATTCCCTGAGGTATTGGAAATATTGCTTAAGGACGACTTTATATCTCAATCAAGGATTGCTTCAGAGGGTTGTCATGGAACTGGTGGTTTCACAATTAGCACAACAATGGGTGAGATTGGAGCTGGTCCAAGTGATTTGCTAAATGAAGCAGAATCGGCAGCTGCCGCTGAAGAAAAAGAAACTCATGCATTTGAAATTGATCCTTCAAAGGTTGAGAATGTTAAGCAGCGTTGCTTGCCAGAATCTTTGAACTATCCCATGTTGGAGGAGTATGACTTCAGAAATGATACAGTTAACCCTGATCTTGACATGGAACTAAAGCCTCAAGCGCAACCACGACCATATCAAGAGAAGAGCTTGAGTAAAATGTTTGGGAACGGGAGAGCAAGATCTGGCATAATTGTACTGCCTTGTGGTGCCGGAAAGTCTCTAGTTGGTGTTTCTGCTGCCTCCAGGATAAGAAAGAGCTGCCTTTGTTTAGCAACAAATGCTGTTTCTGTTGATCAGTGGGCTTTTCAGTTTAAGTTGTGGTCAACCATCCGAGATGAACAAATTTGTCGTTTCACATCTGATAGTAAAGAAAGAATCCGTGGAAATGCTGGGGTAGTTGTGACAACATATAACATGATTGGTTTTGGTGGCAAAAGGTCTGAAGAATCTGAAAAGATCATTGAAGAAATAAGAAACAGAGAATGGGGATTGCTTCTCATGGATGAGGTGCACGTCGTTCCTGCTGAAATGTTTCGAAATGTCATCAGGCTTACTAAATCTCACTGCAAGCTTGGTCTCACCGCCACACTTGTGAGAGAGGATGAGAAGATCACAGACCTTAACTTCCTTATTGGTCCAAAGTTGTATGAAGCAAACTGGTTGGATTTGGTTAAAGGTGGATTTATTGCAAACGTACAGTGTGCTGAAGTATGGTGTCCAATGACAAAAGAGTTTTTTGTGGAATATTTGAAGAAAGAAAACTCAAAGAAAAAGCAGGCACTTTATGTCATGAATCCAAATAAATTTAGGGCATGCGAGTTTCTTATAAGGTTTCATGAAGAGCAACGTGGTGATAAAATTATTGTGTTTGCTGACAATCTCTTTGCCCTCGCGGAGTATGCAAAGAAACTTCGGAAACCTATGATCTGTGGTAGTACCAGCCATGTGGAGAGGACGAAAATTCTAGAAGCCTTCAAAACAAGTCAGGACATAAATACTGTTTTCTTGTCTAAGGTGGGTGATAATTCCATAGACATTCCTGAGGCAAATGTGATTATTCAGATTTCATCACATGCTGGTTCAAGACGTCAAGAAGCACAGCGTCTTGGTCGTATTTTAAGAGCTAAGGAGGAAAAGAGGAGTACATGCAATGCATTTTTTTATTCCCTCGTGTCTACGGATACTCAGGAAATGTACTTTTCAACTAAACGGCAGCAGTTTTTGATTGATCAAGGTTATAGTTTTAAGGTTATTACAAGCTTGCCTCCTCCTGAATCAGGAGCTGATTTGAGCTATCATCGTGTTGAAGACCAACTGGCACTACTCTCAAAGGTGTTGAGTGCTGGTGATGATAAAGTTGGTTTAGAGCCACATGACGATGATGCAGATGATATTTCCCTTCAGAAAGCACGTCGTCCAACAGGATCCATGAGTGTGATGTCGGGTTCAGATGGGATGGTTTACATGGAATATAGTAATGAAAAACACAAGCACAGCAAAGTACAGGTGAAGAGTAAGCCAAGGGATCCGGCAAAGAGACATCATTTGTTTAAACAACGCCGTGGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0008150 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0010224 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0050896

KEGG Pathways

Metabolic & Signaling

ko03022 ko03420 map03022 map03420

Pfam Domains

Protein Families

ERCC3_RAD25_C Helicase_C_3 ResIII

Protein Analysis

760

Amino Acids

85.87

Weight (kDa)

7.81

Isoelectric Point (pI)

38.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Helicase_C_3	PF13625	64 - 187	2.6e-31	Helicase conserved C-terminal domain
ResIII	PF04851	279 - 436	6.8e-18	Type III restriction enzyme, res subunit
ERCC3_RAD25_C	PF16203	461 - 700	1e-115	ERCC3/RAD25/XPB C-terminal helicase
Helicase_C	PF00271	511 - 613	9.2e-13	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0001033)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G41360 AT5G41360 AT5G41370
fragaria_vesca	FvH4_5g02840 FvH4_5g02840 FvH4_5g02840 FvH4_5g02840 FvH4_5g02840 FvH4_5g19420 FvH4_5g19430 FvH4_7g08220 FvH4_7g08220
malus_domestica	MD06G1087900.v1.1
prunus_persica	Prupe.5G104000_v2.0.a1
pyrus_communis	pycom06g08400
rosa_chinensis	RchiOBHm_Chr1g0341611 RchiOBHm_Chr1g0341621 RchiOBHm_Chr1g0341861 RchiOBHm_Chr7g0197061 RchiOBHm_Chr7g0203931
rosa_laevigata	RLG00000003497 RLG00000003962 RLG00000029105 RLG00000029132
rosa_multiflora	Rmu_co8328521.1_g000001 Rmu_co8329503.1_g000001 Rmu_sc0002302.1_g000034 Rmu_sc0002322.1_g000070 Rmu_sc0003411.1_g000004 Rmu_sc0013871.1_g000001 Rmu_sc0032022.1_g000001
rosa_roxburghii	Rroxscaffold_3G00258690 Rroxscaffold_4G00312270 Rroxscaffold_4G00312590
rosa_rugosa	Rorug01G0154800.1 Rorug07G0037400 Rorug07G0079500
rosa_samantha	Rh1AG168500 Rh1AG170500 Rh1BG136800 Rh1BG138300 Rh1BG138400 Rh1CG156100 Rh1CG158700 Rh1DG170300 Rh1DG170600 Rh1DG170700 Rh7AG162300 Rh7AG209400 Rh7AG209500 Rh7BG164400 Rh7BG208200 Rh7BG208300 Rh7CG170500 Rh7CG221900 Rh7DG163800 Rh7DG216500
rosa_wichuraiana	Rw1G014160 Rw1G014180 Rw7G014170 Rw7G018250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1541
AatII	GACGTC	1 cut(s)	1804
Acc36I	ACCTGC	1 cut(s)	414
AccB1I	GGYRCC	1 cut(s)	920
AccI	GTMKAC	1 cut(s)	1889
AccII	CGCG	1 cut(s)	1607
AciI	CCGC	7 cut(s)	96, 184, 323, 338, 669, 1296, 1607
AclWI	GGATC	5 cut(s)	698, 2133, 2146, 2234, 2247
AcsI	RAATTY	3 cut(s)	1056, 1517, 1674
AcuI	CTGAAG	7 cut(s)	482, 549, 693, 766, 1176, 1440, 2099
AcyI	GRCGYC	1 cut(s)	1801
AfaI	GTAC	8 cut(s)	84, 242, 909, 1410, 1651, 1858, 1910, 2217
AfiI	CCNNNNNNNGG	3 cut(s)	125, 193, 1991
AflII	CTTAAG	2 cut(s)	473, 530
AjiI	CACGTC	2 cut(s)	1228, 2126
AjnI	CCWGG	1 cut(s)	956
AleI	CACNNNNGTG	1 cut(s)	1304
Alw21I	GWGCWC	1 cut(s)	1227
Alw26I	GTCTC	4 cut(s)	359, 936, 1295, 2245
Alw44I	GTGCAC	1 cut(s)	1223
AlwI	GGATC	5 cut(s)	698, 2133, 2146, 2234, 2247
AlwNI	CAGNNNCTG	1 cut(s)	1997
AoxI	GGCC	1 cut(s)	19
ApaLI	GTGCAC	1 cut(s)	1223
ApeKI	GCWGC	7 cut(s)	93, 662, 665, 730, 950, 972, 1927
ApoI	RAATTY	3 cut(s)	1056, 1517, 1674
ArsI	GACNNNNNNTTYG	4 cut(s)	310, 342, 1580, 1612
Asp700I	GAANNNNTTC	1 cut(s)	1245
AspLEI	GCGC	1 cut(s)	835
AspS9I	GGNCC	2 cut(s)	629, 1349
AsuHPI	GGTGA	9 cut(s)	23, 110, 140, 626, 1285, 1577, 1745, 2072, 2233
AsuII	TTCGAA	1 cut(s)	1249
AvaII	GGWCC	2 cut(s)	629, 1349
AxyI	CCTNAGG	2 cut(s)	510, 1755
BaeGI	GKGCMC	1 cut(s)	1227
BamHI	GGATCC	2 cut(s)	2138, 2239
BanI	GGYRCC	1 cut(s)	920
BarI	GAAGNNNNNNTAC	2 cut(s)	470, 502
BauI	CACGAG	3 cut(s)	305, 467, 1883
BbsI	GAAGAC	2 cut(s)	114, 2028
Bbv12I	GWGCWC	1 cut(s)	1227
BbvI	GCAGC	7 cut(s)	105, 652, 674, 742, 937, 959, 1939
BccI	CCATC	4 cut(s)	200, 1049, 2159, 2164
BceAI	ACGGC	2 cut(s)	1940, 2259
BciT130I	CCWGG	1 cut(s)	958
BciVI	GTATCC	1 cut(s)	1888
BclI	TGATCA	2 cut(s)	1006, 1942
BcoDI	GTCTC	4 cut(s)	359, 936, 1295, 2245
BfaI	CTAG	3 cut(s)	158, 935, 1679
BfmI	CTRYAG	1 cut(s)	91
BfrI	CTTAAG	2 cut(s)	473, 530
BfuAI	ACCTGC	1 cut(s)	414
BfuI	GTATCC	1 cut(s)	1888
BglII	AGATCT	1 cut(s)	893
BisI	GCNGC	9 cut(s)	94, 339, 663, 666, 669, 731, 951, 973, 1928
BlsI	GCNGC	9 cut(s)	95, 340, 664, 667, 670, 732, 952, 974, 1929
Bme1390I	CCNGG	1 cut(s)	958
Bme18I	GGWCC	2 cut(s)	629, 1349
BmgBI	CACGTC	2 cut(s)	1228, 2126
BmgT120I	GGNCC	2 cut(s)	629, 1349
BmiI	GGNNCC	3 cut(s)	922, 2140, 2241
BmrFI	CCNGG	1 cut(s)	958
BmsI	GCATC	2 cut(s)	291, 2086
BpiI	GAAGAC	2 cut(s)	114, 2028
BpmI	CTGGAG	1 cut(s)	940
Bpu10I	CCTNAGC	1 cut(s)	1839
Bpu14I	TTCGAA	1 cut(s)	1249
BpuEI	CTTGAG	2 cut(s)	813, 883
BsaHI	GRCGYC	1 cut(s)	1801
BsaI	GGTCTC	2 cut(s)	359, 1295
BsaJI	CCNNGG	4 cut(s)	121, 1093, 2234, 2275
Bsc4I	CCNNNNNNNGG	3 cut(s)	125, 193, 1991
Bse1I	ACTGG	3 cut(s)	589, 1376, 2034
Bse21I	CCTNAGG	2 cut(s)	510, 1755
Bse3DI	GCAATG	3 cut(s)	264, 270, 1870
BseBI	CCWGG	1 cut(s)	958
BseDI	CCNNGG	4 cut(s)	121, 1093, 2234, 2275
BseGI	GGATG	4 cut(s)	1041, 1222, 1321, 2175
BseLI	CCNNNNNNNGG	3 cut(s)	125, 193, 1991
BseMI	GCAATG	3 cut(s)	264, 270, 1870
BseMII	CTCAG	4 cut(s)	52, 501, 1746, 1913
BseNI	ACTGG	3 cut(s)	589, 1376, 2034
BseRI	GAGGAG	3 cut(s)	785, 1867, 1971
BseSI	GKGCMC	1 cut(s)	1227
BseXI	GCAGC	7 cut(s)	105, 652, 674, 742, 937, 959, 1939
BseYI	CCCAGC	1 cut(s)	1103
Bsh1236I	CGCG	1 cut(s)	1607
BshFI	GGCC	1 cut(s)	21
BshNI	GGYRCC	1 cut(s)	920
BsiHKAI	GWGCWC	1 cut(s)	1227
BsiSI	CCGG	2 cut(s)	924, 2243
BslFI	GGGAC	2 cut(s)	18, 101
BslI	CCNNNNNNNGG	3 cut(s)	125, 193, 1991
BsmAI	GTCTC	4 cut(s)	359, 936, 1295, 2245
BsmFI	GGGAC	2 cut(s)	18, 101
BsmI	GAATGC	1 cut(s)	325
BsnI	GGCC	1 cut(s)	21
Bso31I	GGTCTC	2 cut(s)	359, 1295
Bsp119I	TTCGAA	1 cut(s)	1249
Bsp1286I	GDGCHC	1 cut(s)	1227
Bsp1407I	TGTACA	1 cut(s)	240
Bsp19I	CCATGG	1 cut(s)	121
BspACI	CCGC	7 cut(s)	96, 184, 323, 338, 669, 1296, 1607
BspANI	GGCC	1 cut(s)	21
BspCNI	CTCAG	4 cut(s)	51, 502, 1747, 1912
BspFNI	CGCG	1 cut(s)	1607
BspHI	TCATGA	2 cut(s)	1503, 1549
BspLI	GGNNCC	3 cut(s)	922, 2140, 2241
BspMAI	CTGCAG	1 cut(s)	95
BspMI	ACCTGC	1 cut(s)	414
BspPI	GGATC	5 cut(s)	698, 2133, 2146, 2234, 2247
BspQI	GCTCTTC	2 cut(s)	851, 1548
BspT104I	TTCGAA	1 cut(s)	1249
BspT107I	GGYRCC	1 cut(s)	920
BspTI	CTTAAG	2 cut(s)	473, 530
BspTNI	GGTCTC	2 cut(s)	359, 1295
BsrDI	GCAATG	3 cut(s)	264, 270, 1870
BsrGI	TGTACA	1 cut(s)	240
BsrI	ACTGG	3 cut(s)	589, 1376, 2034
BssECI	CCNNGG	4 cut(s)	121, 1093, 2234, 2275
BssNI	GRCGYC	1 cut(s)	1801
BssSI	CACGAG	3 cut(s)	305, 467, 1883
BssT1I	CCWWGG	2 cut(s)	121, 2234
Bst2BI	CACGAG	3 cut(s)	305, 467, 1883
Bst2UI	CCWGG	1 cut(s)	958
Bst4CI	ACNGT	4 cut(s)	11, 796, 1413, 1714
Bst6I	CTCTTC	3 cut(s)	851, 1548, 2219
BstACI	GRCGYC	1 cut(s)	1801
BstAFI	CTTAAG	2 cut(s)	473, 530
BstAPI	GCANNNNNTGC	1 cut(s)	992
BstAUI	TGTACA	1 cut(s)	240
BstBI	TTCGAA	1 cut(s)	1249
BstC8I	GCNNGC	7 cut(s)	199, 290, 740, 1283, 1491, 1528, 1976
BstDEI	CTNAG	6 cut(s)	38, 510, 1725, 1755, 1839, 1899
BstDSI	CCRYGG	3 cut(s)	121, 1093, 2275
BstENI	CCTNNNNNAGG	1 cut(s)	1989
BstF5I	GGATG	4 cut(s)	1041, 1222, 1321, 2175
BstFNI	CGCG	1 cut(s)	1607
BstHHI	GCGC	1 cut(s)	835
BstMAI	GTCTC	4 cut(s)	359, 936, 1295, 2245
BstMWI	GCNNNNNNNGC	8 cut(s)	388, 443, 659, 668, 739, 832, 992, 1613
BstNI	CCWGG	1 cut(s)	958
BstNSI	RCATGY	3 cut(s)	1530, 1790, 1863
BstSCI	CCNGG	1 cut(s)	956
BstSFI	CTRYAG	1 cut(s)	91
BstSLI	GKGCMC	1 cut(s)	1227
BstUI	CGCG	1 cut(s)	1607
BstV1I	GCAGC	7 cut(s)	105, 652, 674, 742, 937, 959, 1939
BstV2I	GAAGAC	2 cut(s)	114, 2028
BstX2I	RGATCY	3 cut(s)	893, 2138, 2239
BstXI	CCANNNNNNTGG	1 cut(s)	1660
BstYI	RGATCY	3 cut(s)	893, 2138, 2239
Bsu36I	CCTNAGG	2 cut(s)	510, 1755
BsuI	GTATCC	1 cut(s)	1888
BsuRI	GGCC	1 cut(s)	21
BtgI	CCRYGG	3 cut(s)	121, 1093, 2275
BtrI	CACGTC	2 cut(s)	1228, 2126
BtsCI	GGATG	4 cut(s)	1041, 1222, 1321, 2175
BtsI	GCAGTG	1 cut(s)	1276
BtsIMutI	CAGTG	3 cut(s)	1016, 1276, 1418
BveI	ACCTGC	1 cut(s)	414
Cac8I	GCNNGC	7 cut(s)	199, 290, 740, 1283, 1491, 1528, 1976
CaiI	CAGNNNCTG	1 cut(s)	1997
CciI	TCATGA	2 cut(s)	1503, 1549
CfoI	GCGC	1 cut(s)	835
Cfr13I	GGNCC	2 cut(s)	629, 1349
CseI	GACGC	1 cut(s)	1805
Csp6I	GTAC	8 cut(s)	83, 241, 908, 1409, 1650, 1857, 1909, 2216
CviQI	GTAC	8 cut(s)	83, 241, 908, 1409, 1650, 1857, 1909, 2216
DdeI	CTNAG	6 cut(s)	38, 510, 1725, 1755, 1839, 1899
DraI	TTTAAA	1 cut(s)	2266
Eam1104I	CTCTTC	3 cut(s)	851, 1548, 2219
EarI	CTCTTC	3 cut(s)	851, 1548, 2219
Eco130I	CCWWGG	2 cut(s)	121, 2234
Eco31I	GGTCTC	2 cut(s)	359, 1295
Eco47I	GGWCC	2 cut(s)	629, 1349
Eco57I	CTGAAG	7 cut(s)	482, 549, 693, 766, 1176, 1440, 2099
Eco81I	CCTNAGG	2 cut(s)	510, 1755
EcoNI	CCTNNNNNAGG	1 cut(s)	1989
EcoRII	CCWGG	1 cut(s)	956
EcoT14I	CCWWGG	2 cut(s)	121, 2234
EcoT22I	ATGCAT	2 cut(s)	694, 1870
ErhI	CCWWGG	2 cut(s)	121, 2234
FalI	AAGNNNNNCTT	4 cut(s)	524, 556, 547, 579
FaqI	GGGAC	2 cut(s)	18, 101
FauI	CCCGC	1 cut(s)	330
FbaI	TGATCA	2 cut(s)	1006, 1942
FblI	GTMKAC	1 cut(s)	1889
Fnu4HI	GCNGC	9 cut(s)	94, 339, 663, 666, 669, 731, 951, 973, 1928
FokI	GGATG	4 cut(s)	1028, 1229, 1328, 2182
Fsp4HI	GCNGC	9 cut(s)	94, 339, 663, 666, 669, 731, 951, 973, 1928
FspBI	CTAG	3 cut(s)	158, 935, 1679
GlaI	GCGC	1 cut(s)	834
GluI	GCNGC	9 cut(s)	94, 339, 663, 666, 669, 731, 951, 973, 1928
GsaI	CCCAGC	1 cut(s)	1107
GsuI	CTGGAG	1 cut(s)	940
HaeIII	GGCC	1 cut(s)	21
HapII	CCGG	2 cut(s)	924, 2243
HgaI	GACGC	1 cut(s)	1805
HhaI	GCGC	1 cut(s)	835
Hin1I	GRCGYC	1 cut(s)	1801
Hin6I	GCGC	1 cut(s)	833
HinP1I	GCGC	1 cut(s)	833
HincII	GTYRAC	3 cut(s)	403, 799, 1038
HindII	GTYRAC	3 cut(s)	403, 799, 1038
HindIII	AAGCTT	3 cut(s)	249, 1283, 1972
HpaI	GTTAAC	1 cut(s)	799
HpaII	CCGG	2 cut(s)	924, 2243
HphI	GGTGA	9 cut(s)	23, 110, 140, 626, 1285, 1577, 1745, 2072, 2233
Hpy166II	GTNNAC	8 cut(s)	85, 241, 403, 799, 1038, 1225, 1890, 2176
Hpy8I	GTNNAC	8 cut(s)	85, 241, 403, 799, 1038, 1225, 1890, 2176
Hpy99I	CGWCG	4 cut(s)	80, 83, 1232, 2130
HpyAV	CCTTC	4 cut(s)	125, 717, 1696, 2123
HpyCH4III	ACNGT	4 cut(s)	11, 796, 1413, 1714
HpyCH4IV	ACGT	6 cut(s)	81, 1227, 1407, 1561, 1801, 2125
HpyF10VI	GCNNNNNNNGC	8 cut(s)	388, 443, 659, 668, 739, 832, 992, 1613
HpyF3I	CTNAG	6 cut(s)	38, 510, 1725, 1755, 1839, 1899
HpySE526I	ACGT	6 cut(s)	81, 1227, 1407, 1561, 1801, 2125
Hsp92I	GRCGYC	1 cut(s)	1801
HspAI	GCGC	1 cut(s)	833
Ksp22I	TGATCA	2 cut(s)	1006, 1942
KspAI	GTTAAC	1 cut(s)	799
LguI	GCTCTTC	2 cut(s)	851, 1548
LmnI	GCTCC	2 cut(s)	623, 1994
Lsp1109I	GCAGC	7 cut(s)	105, 652, 674, 742, 937, 959, 1939
LweI	GCATC	2 cut(s)	291, 2086
MaeI	CTAG	3 cut(s)	158, 935, 1679
MaeII	ACGT	6 cut(s)	81, 1227, 1407, 1561, 1801, 2125
MaeIII	GTNAC	2 cut(s)	11, 1114
MflI	RGATCY	3 cut(s)	893, 2138, 2239
MhlI	GDGCHC	1 cut(s)	1227
MlyI	GAGTC	1 cut(s)	36
MmeI	TCCRAC	3 cut(s)	747, 1356, 2156
Mph1103I	ATGCAT	2 cut(s)	694, 1870
MroXI	GAANNNNTTC	1 cut(s)	1245
MslI	CAYNNNNRTG	5 cut(s)	126, 608, 1304, 2091, 2147
MspA1I	CMGCKG	3 cut(s)	96, 665, 671
MspCI	CTTAAG	2 cut(s)	473, 530
MspI	CCGG	2 cut(s)	924, 2243
MspR9I	CCNGG	1 cut(s)	958
MssI	GTTTAAAC	1 cut(s)	2266
Mva1269I	GAATGC	1 cut(s)	325
MvaI	CCWGG	1 cut(s)	958
MvnI	CGCG	1 cut(s)	1607
MwoI	GCNNNNNNNGC	8 cut(s)	388, 443, 659, 668, 739, 832, 992, 1613
NcoI	CCATGG	1 cut(s)	121
NlaIV	GGNNCC	3 cut(s)	922, 2140, 2241
NmeAIII	GCCGAG	1 cut(s)	435
NmuCI	GTSAC	2 cut(s)	11, 1114
NsiI	ATGCAT	2 cut(s)	694, 1870
NspI	RCATGY	3 cut(s)	1530, 1790, 1863
NspV	TTCGAA	1 cut(s)	1249
OliI	CACNNNNGTG	1 cut(s)	1304
PaeI	GCATGC	1 cut(s)	1530
PagI	TCATGA	2 cut(s)	1503, 1549
PciSI	GCTCTTC	2 cut(s)	851, 1548
PctI	GAATGC	1 cut(s)	325
PdmI	GAANNNNTTC	1 cut(s)	1245
PfeI	GAWTC	9 cut(s)	224, 481, 497, 656, 746, 1089, 1160, 1507, 1988
PflFI	GACNNNGTC	1 cut(s)	113
PfoI	TCCNGGA	1 cut(s)	956
PkrI	GCNGC	9 cut(s)	95, 340, 664, 667, 670, 732, 952, 974, 1929
PleI	GAGTC	1 cut(s)	36
PmeI	GTTTAAAC	1 cut(s)	2266
PpsI	GAGTC	1 cut(s)	36
PsiI	TTATAA	1 cut(s)	1541
Psp6I	CCWGG	1 cut(s)	956
PspFI	CCCAGC	1 cut(s)	1103
PspGI	CCWGG	1 cut(s)	956
PspN4I	GGNNCC	3 cut(s)	922, 2140, 2241
PspPI	GGNCC	2 cut(s)	629, 1349
PstI	CTGCAG	1 cut(s)	95
PstNI	CAGNNNCTG	1 cut(s)	1997
PsuI	RGATCY	3 cut(s)	893, 2138, 2239
PsyI	GACNNNGTC	1 cut(s)	113
PvuII	CAGCTG	1 cut(s)	665
RsaI	GTAC	8 cut(s)	84, 242, 909, 1410, 1651, 1858, 1910, 2217
RsaNI	GTAC	8 cut(s)	83, 241, 908, 1409, 1650, 1857, 1909, 2216
RseI	CAYNNNNRTG	5 cut(s)	126, 608, 1304, 2091, 2147
SapI	GCTCTTC	2 cut(s)	851, 1548
SatI	GCNGC	9 cut(s)	94, 339, 663, 666, 669, 731, 951, 973, 1928
Sau96I	GGNCC	2 cut(s)	629, 1349
SchI	GAGTC	1 cut(s)	36
ScrFI	CCNGG	1 cut(s)	958
SduI	GDGCHC	1 cut(s)	1227
SfaNI	GCATC	2 cut(s)	291, 2086
SfcI	CTRYAG	1 cut(s)	91
SfuI	TTCGAA	1 cut(s)	1249
SinI	GGWCC	2 cut(s)	629, 1349
SmiMI	CAYNNNNRTG	5 cut(s)	126, 608, 1304, 2091, 2147
SmlI	CTYRAG	4 cut(s)	473, 530, 828, 862
SmoI	CTYRAG	4 cut(s)	473, 530, 828, 862
SphI	GCATGC	1 cut(s)	1530
SsiI	CCGC	7 cut(s)	96, 184, 323, 338, 669, 1296, 1607
SspI	AATATT	3 cut(s)	216, 525, 1460
SspMI	CTAG	3 cut(s)	158, 935, 1679
StyD4I	CCNGG	1 cut(s)	956
StyI	CCWWGG	2 cut(s)	121, 2234
TaaI	ACNGT	4 cut(s)	11, 796, 1413, 1714
TaiI	ACGT	6 cut(s)	84, 1230, 1410, 1564, 1804, 2128
TaqI	TCGA	3 cut(s)	211, 222, 1249
TatI	WGTACW	5 cut(s)	240, 907, 1856, 1908, 2215
TauI	GCSGC	2 cut(s)	341, 671
TfiI	GAWTC	9 cut(s)	224, 481, 497, 656, 746, 1089, 1160, 1507, 1988
TscAI	CASTG	3 cut(s)	1016, 1283, 1418
TseFI	GTSAC	2 cut(s)	11, 1114
TseI	GCWGC	7 cut(s)	93, 662, 665, 730, 950, 972, 1927
Tsp45I	GTSAC	2 cut(s)	11, 1114
TspDTI	ATGAA	9 cut(s)	418, 663, 1065, 1377, 1520, 1538, 1566, 1770, 2208
TspGWI	ACGGA	3 cut(s)	377, 1082, 1907
TspRI	CASTG	3 cut(s)	1016, 1283, 1418
Tth111I	GACNNNGTC	1 cut(s)	113
Vha464I	CTTAAG	2 cut(s)	473, 530
VneI	GTGCAC	1 cut(s)	1223
VpaK11BI	GGWCC	2 cut(s)	629, 1349
XagI	CCTNNNNNAGG	1 cut(s)	1989
XapI	RAATTY	3 cut(s)	1056, 1517, 1674
XbaI	TCTAGA	1 cut(s)	1678
XceI	RCATGY	3 cut(s)	1530, 1790, 1863
XmiI	GTMKAC	1 cut(s)	1889
XmnI	GAANNNNTTC	1 cut(s)	1245
XspI	CTAG	3 cut(s)	158, 935, 1679
ZraI	GACGTC	1 cut(s)	1802
Zsp2I	ATGCAT	2 cut(s)	694, 1870

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.