RLG00000029105

DNA repair helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0001033
Physical Location & Seq
Reverse (-)
33060231 .. 33066260
6030 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000029105

Sequence Viewer

Length: 2304 bp
ATGGGACACGGTGACAAAGGTCGACCCAGTAAAAAGCTCCGACTCTCTATCAAGGAAAAATCAAATGTGCAAAACGACGACGTACACTACTGCAGCGGTGAGGAAGAAGACAATGTCCACCATGGTGGTGAAGGGAAATACAGAGATTTTACCAAACTAGAATTAAAGCCTGACCACCCTAACCGCCCTATATGGGCTTGCCCTGATGGCCGAATATTCCTCGAATCTTTCTCCGCTCTGTACAAACAAGCTTATGATTTCCTCATTGCCATTGCCGAACCTGTTTGCAGGCAAGAGTTGATGCACGAGTATAACTTGACCCCGCATTCGCTGTATGCTGCTGTGTCAGTTGGTCTTGCTACGGAGACCATTATTTCTGTGCTTAATAAGCTGTCAAAGGTCAACCTGCCGAGAGAAGTTATTGATTTCATACACGCTTCTACTGCTAATTATGGCAAACTGAAGCTCGTGCTTAAGAAGAATCGCTACTTTGTGGAATCCCCATTCCCTGAGGTATTGGAAATATTGCTTAAGGACGACTTTATATCTCAATCAAGGATTGCTTCAGAGGGTTCTCATGGAACTGGTGGTTTCACAATTAGCACAACAATGGGTGAGATTGGAGCTGGTCCAAGTGATTTGCTAAATGAAGCAGAATTGGCAGCTGCCGCTGAAGAAAAAGAAACTCATGCATTTGAAATTGATCCTTCAAAGGTTGAGAATGTTAAGCAGCGTTGCTTGCCAGAATCTTTGAACTATCCCATGTTGGAGGAGTATGACTTCAGAAATGATACAGTTAACCCTGATCTTGACATGGAACTAAAGCCTCAAGCGCAACCACGACCATATCAGGAGAAGAGCTTGAGTAAAATGTTTGGGAACGGGAGAGCAAGATCTGGCATAATTGTACTGCCTTGTGGTGCCGGAAAGTCTCTAGTTGGTGTTTCTGCTGCCTCCAGGATAAGAAAGAGCTGCCTTTGTTTAGCAACAAATGCTGTTTCTGATGATCAGTGGGCTTTTCAGTTTAAGTTGTGGTCAACCATCCGAGATGAACAAATTTGTCGTTTCACATCTGATAGTAAAGAAAGAATCCGTGGAAATGCTGGGGTAGTTGTGACAACATATAACATGATTGGTTTTGGTGGCAAGAGGTCTGAAGAATCTGAAAAGACCATTGAAGAAATAAGAAACAGAGAATGGGGATTGCTTCTCATGGATGAGGTGCACGTCGTTCCTGCTGAAATGTTTCGAAATGTCATCAGGCTTACTAAATCTCACTGCAAGCTTGGTCTCACCGCCACACTTGTGAGAGAGGATGAGAAGATCACAGACCTTAACTTCCTTATTGGTCCAAAGCTGTATGAAGCAAACTGGTTGGATTTGGTTAAAGGTGGATTTATTGCAAATGTACAGTGTGCTGAAGTATGGTGTCCAATGACAAAAGAGTTTTTTGTGGAATATTTGAAGAAAGAAAACTCAAAGAAAAAGCAGGCACTTTATGTCATGAATCCAAATAAGTTTAGGGCATGCGAGTTTCTTATAAGGTTTCATGAAGAGCAACGTGGTGATAAAATTATTGTGTTTGCTGACAATCTCTTTGCCCTTGCGGAGTATGCGAAGAAACTTCGGAAACCTATGATCTGTGGTAGTACCAGCCATGTGGAGAGGACGAAAATTCTAGAAGCCTTCAAAACAAGTCAGGACATAAATACTGTTTTCTTGTCTAAGGTGGGTGATAATTCCATAGACATTCCTGAGGCAAATGTGATTATTCAGATGTCATCACATGCTGGTTCAAGACGTCAAGAAGCACAGCGTCTTGGTCGTATTTTAAGAGCTAAGGGTAGACCTCAGGATCGGATAGCAGGAGGAAAAGAGGAGTACAATGCATTTTTTTATTCCCTCGTGTCTACGGATACTCAGGAAATGTACTTTTCAACTAAACGGCAGCAGTTTTTGATTGATCAAGGTTATAGTTTTAAGGTTATTACAAGCTTGCCTCCTCCTGAATCAGGAGCTGATTTGAGCTATCATCGTGTTGAAGACCAACAGGCACTACTCTCAAAGGTGTTGAGTGCTGGTGATGATAAAGTTGGTTTAGAGCCACATGACGATGATGCAGATGATATTTCCCTTCAGAAAGCACGTCGTTCAACAGGATCCATGAGTGTGATGTCGGGTTCAGATGGGATGGTTTACATGGAATACAGTAATGAAAAACACAAGCACAGCAAAGTACAGGTGAAGAGTAAGCCAAGGGATCCGGCAAAGAGACATCATTTGTTTAAACAACGCCGTGGCTGA

Protein Analysis

768

Amino Acids

86.65

Weight (kDa)

7.58

Isoelectric Point (pI)

38.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helicase_C_3 PF13625 64 - 187 1.3e-31 Helicase conserved C-terminal domain
ResIII PF04851 279 - 436 1e-17 Type III restriction enzyme, res subunit
ERCC3_RAD25_C PF16203 461 - 707 4.4e-115 ERCC3/RAD25/XPB C-terminal helicase
Helicase_C PF00271 511 - 613 5.4e-13 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1541
AatII GACGTC 1 cut(s) 1804
Acc36I ACCTGC 1 cut(s) 414
AccB1I GGYRCC 1 cut(s) 920
AccBSI CCGCTC 1 cut(s) 236
AccI GTMKAC 3 cut(s) 22, 1846, 1910
AciI CCGC 7 cut(s) 96, 184, 234, 323, 669, 1296, 1607
AclWI GGATC 6 cut(s) 698, 1863, 2154, 2167, 2255, 2268
AcoI YGGCCR 1 cut(s) 208
AcsI RAATTY 2 cut(s) 1056, 1674
AcuI CTGAAG 7 cut(s) 482, 549, 693, 766, 1176, 1440, 2120
AcyI GRCGYC 1 cut(s) 1801
AfaI GTAC 8 cut(s) 84, 242, 909, 1410, 1651, 1883, 1931, 2238
AfiI CCNNNNNNNGG 2 cut(s) 193, 2012
AflII CTTAAG 2 cut(s) 473, 530
AjiI CACGTC 2 cut(s) 1228, 2147
AjnI CCWGG 1 cut(s) 956
AleI CACNNNNGTG 2 cut(s) 123, 1304
Alw21I GWGCWC 1 cut(s) 1227
Alw26I GTCTC 4 cut(s) 359, 936, 1295, 2266
Alw44I GTGCAC 1 cut(s) 1223
AlwI GGATC 6 cut(s) 698, 1863, 2154, 2167, 2255, 2268
AlwNI CAGNNNCTG 1 cut(s) 2018
AoxI GGCC 1 cut(s) 208
ApaLI GTGCAC 1 cut(s) 1223
ApeKI GCWGC 8 cut(s) 93, 338, 662, 665, 730, 950, 972, 1948
ApoI RAATTY 2 cut(s) 1056, 1674
ArsI GACNNNNNNTTYG 4 cut(s) 310, 342, 1580, 1612
Asp700I GAANNNNTTC 1 cut(s) 1245
AspLEI GCGC 1 cut(s) 835
AspS9I GGNCC 2 cut(s) 629, 1349
AsuHPI GGTGA 9 cut(s) 23, 110, 140, 626, 1285, 1577, 1745, 2093, 2254
AsuII TTCGAA 1 cut(s) 1249
AvaII GGWCC 2 cut(s) 629, 1349
AxyI CCTNAGG 3 cut(s) 510, 1755, 1851
BaeGI GKGCMC 1 cut(s) 1227
BamHI GGATCC 2 cut(s) 2159, 2260
BanI GGYRCC 1 cut(s) 920
BarI GAAGNNNNNNTAC 2 cut(s) 470, 502
BauI CACGAG 3 cut(s) 305, 467, 1904
BbsI GAAGAC 2 cut(s) 114, 2049
Bbv12I GWGCWC 1 cut(s) 1227
BbvI GCAGC 8 cut(s) 105, 325, 652, 674, 742, 937, 959, 1960
BccI CCATC 4 cut(s) 200, 1049, 2180, 2185
BceAI ACGGC 2 cut(s) 1961, 2280
BciT130I CCWGG 1 cut(s) 958
BciVI GTATCC 1 cut(s) 1909
BclI TGATCA 2 cut(s) 1006, 1963
BcoDI GTCTC 4 cut(s) 359, 936, 1295, 2266
BfaI CTAG 3 cut(s) 158, 935, 1679
BfmI CTRYAG 1 cut(s) 91
BfrI CTTAAG 2 cut(s) 473, 530
BfuAI ACCTGC 1 cut(s) 414
BfuI GTATCC 1 cut(s) 1909
BglI GCCNNNNNGGC 1 cut(s) 207
BglII AGATCT 1 cut(s) 893
BisI GCNGC 9 cut(s) 94, 339, 663, 666, 669, 731, 951, 973, 1949
BlsI GCNGC 9 cut(s) 95, 340, 664, 667, 670, 732, 952, 974, 1950
Bme1390I CCNGG 1 cut(s) 958
Bme18I GGWCC 2 cut(s) 629, 1349
BmgBI CACGTC 2 cut(s) 1228, 2147
BmgT120I GGNCC 2 cut(s) 629, 1349
BmiI GGNNCC 3 cut(s) 922, 2161, 2262
BmrFI CCNGG 1 cut(s) 958
BmrI ACTGGG 1 cut(s) 21
BmsI GCATC 2 cut(s) 291, 2107
BmuI ACTGGG 1 cut(s) 21
BoxI GACNNNNGTC 1 cut(s) 18
BpiI GAAGAC 2 cut(s) 114, 2049
BpmI CTGGAG 1 cut(s) 940
Bpu10I CCTNAGC 1 cut(s) 1839
Bpu14I TTCGAA 1 cut(s) 1249
BpuEI CTTGAG 2 cut(s) 813, 883
BsaHI GRCGYC 1 cut(s) 1801
BsaI GGTCTC 2 cut(s) 359, 1295
BsaJI CCNNGG 4 cut(s) 121, 1093, 2255, 2296
Bsc4I CCNNNNNNNGG 2 cut(s) 193, 2012
Bse1I ACTGG 3 cut(s) 27, 589, 1376
Bse21I CCTNAGG 3 cut(s) 510, 1755, 1851
Bse3DI GCAATG 2 cut(s) 264, 270
BseBI CCWGG 1 cut(s) 958
BseDI CCNNGG 4 cut(s) 121, 1093, 2255, 2296
BseGI GGATG 4 cut(s) 1041, 1222, 1321, 2196
BseLI CCNNNNNNNGG 2 cut(s) 193, 2012
BseMI GCAATG 2 cut(s) 264, 270
BseMII CTCAG 4 cut(s) 501, 1746, 1865, 1934
BseNI ACTGG 3 cut(s) 27, 589, 1376
BseRI GAGGAG 3 cut(s) 785, 1892, 1992
BseSI GKGCMC 1 cut(s) 1227
BseXI GCAGC 8 cut(s) 105, 325, 652, 674, 742, 937, 959, 1960
BseYI CCCAGC 1 cut(s) 1103
BshFI GGCC 1 cut(s) 210
BshNI GGYRCC 1 cut(s) 920
BsiHKAI GWGCWC 1 cut(s) 1227
BsiSI CCGG 2 cut(s) 924, 2264
BslFI GGGAC 1 cut(s) 18
BslI CCNNNNNNNGG 2 cut(s) 193, 2012
BsmAI GTCTC 4 cut(s) 359, 936, 1295, 2266
BsmFI GGGAC 1 cut(s) 18
BsmI GAATGC 1 cut(s) 325
BsnI GGCC 1 cut(s) 210
Bso31I GGTCTC 2 cut(s) 359, 1295
Bsp119I TTCGAA 1 cut(s) 1249
Bsp1286I GDGCHC 1 cut(s) 1227
Bsp1407I TGTACA 2 cut(s) 240, 1408
Bsp19I CCATGG 1 cut(s) 121
BspACI CCGC 7 cut(s) 96, 184, 234, 323, 669, 1296, 1607
BspANI GGCC 1 cut(s) 210
BspCNI CTCAG 4 cut(s) 502, 1747, 1864, 1933
BspHI TCATGA 2 cut(s) 1503, 1549
BspLI GGNNCC 3 cut(s) 922, 2161, 2262
BspMAI CTGCAG 1 cut(s) 95
BspMI ACCTGC 1 cut(s) 414
BspPI GGATC 6 cut(s) 698, 1863, 2154, 2167, 2255, 2268
BspQI GCTCTTC 2 cut(s) 851, 1548
BspT104I TTCGAA 1 cut(s) 1249
BspT107I GGYRCC 1 cut(s) 920
BspTI CTTAAG 2 cut(s) 473, 530
BspTNI GGTCTC 2 cut(s) 359, 1295
BsrBI CCGCTC 1 cut(s) 236
BsrDI GCAATG 2 cut(s) 264, 270
BsrGI TGTACA 2 cut(s) 240, 1408
BsrI ACTGG 3 cut(s) 27, 589, 1376
BssECI CCNNGG 4 cut(s) 121, 1093, 2255, 2296
BssNI GRCGYC 1 cut(s) 1801
BssSI CACGAG 3 cut(s) 305, 467, 1904
BssT1I CCWWGG 2 cut(s) 121, 2255
Bst2BI CACGAG 3 cut(s) 305, 467, 1904
Bst2UI CCWGG 1 cut(s) 958
Bst4CI ACNGT 5 cut(s) 11, 796, 1413, 1714, 2210
Bst6I CTCTTC 3 cut(s) 851, 1548, 2240
BstACI GRCGYC 1 cut(s) 1801
BstAFI CTTAAG 2 cut(s) 473, 530
BstAPI GCANNNNNTGC 1 cut(s) 992
BstAUI TGTACA 2 cut(s) 240, 1408
BstBI TTCGAA 1 cut(s) 1249
BstC8I GCNNGC 7 cut(s) 199, 290, 740, 1283, 1491, 1528, 1997
BstDEI CTNAG 6 cut(s) 510, 1725, 1755, 1839, 1851, 1920
BstDSI CCRYGG 3 cut(s) 121, 1093, 2296
BstENI CCTNNNNNAGG 1 cut(s) 2010
BstF5I GGATG 4 cut(s) 1041, 1222, 1321, 2196
BstHHI GCGC 1 cut(s) 835
BstMAI GTCTC 4 cut(s) 359, 936, 1295, 2266
BstMWI GCNNNNNNNGC 9 cut(s) 207, 388, 443, 659, 668, 739, 832, 992, 1613
BstNI CCWGG 1 cut(s) 958
BstNSI RCATGY 2 cut(s) 1530, 1790
BstPAI GACNNNNGTC 1 cut(s) 18
BstSCI CCNGG 1 cut(s) 956
BstSFI CTRYAG 1 cut(s) 91
BstSLI GKGCMC 1 cut(s) 1227
BstV1I GCAGC 8 cut(s) 105, 325, 652, 674, 742, 937, 959, 1960
BstV2I GAAGAC 2 cut(s) 114, 2049
BstX2I RGATCY 3 cut(s) 893, 2159, 2260
BstXI CCANNNNNNTGG 2 cut(s) 125, 1660
BstYI RGATCY 3 cut(s) 893, 2159, 2260
Bsu36I CCTNAGG 3 cut(s) 510, 1755, 1851
BsuI GTATCC 1 cut(s) 1909
BsuRI GGCC 1 cut(s) 210
BtgI CCRYGG 3 cut(s) 121, 1093, 2296
BtrI CACGTC 2 cut(s) 1228, 2147
BtsCI GGATG 4 cut(s) 1041, 1222, 1321, 2196
BtsI GCAGTG 1 cut(s) 1276
BtsIMutI CAGTG 3 cut(s) 1016, 1276, 1418
BveI ACCTGC 1 cut(s) 414
Cac8I GCNNGC 7 cut(s) 199, 290, 740, 1283, 1491, 1528, 1997
CaiI CAGNNNCTG 1 cut(s) 2018
CciI TCATGA 2 cut(s) 1503, 1549
CfoI GCGC 1 cut(s) 835
Cfr13I GGNCC 2 cut(s) 629, 1349
CseI GACGC 1 cut(s) 1805
Csp6I GTAC 8 cut(s) 83, 241, 908, 1409, 1650, 1882, 1930, 2237
CviQI GTAC 8 cut(s) 83, 241, 908, 1409, 1650, 1882, 1930, 2237
DdeI CTNAG 6 cut(s) 510, 1725, 1755, 1839, 1851, 1920
DraI TTTAAA 1 cut(s) 2287
EaeI YGGCCR 1 cut(s) 208
Eam1104I CTCTTC 3 cut(s) 851, 1548, 2240
EarI CTCTTC 3 cut(s) 851, 1548, 2240
Eco130I CCWWGG 2 cut(s) 121, 2255
Eco31I GGTCTC 2 cut(s) 359, 1295
Eco47I GGWCC 2 cut(s) 629, 1349
Eco57I CTGAAG 7 cut(s) 482, 549, 693, 766, 1176, 1440, 2120
Eco81I CCTNAGG 3 cut(s) 510, 1755, 1851
EcoNI CCTNNNNNAGG 1 cut(s) 2010
EcoRII CCWGG 1 cut(s) 956
EcoT14I CCWWGG 2 cut(s) 121, 2255
EcoT22I ATGCAT 2 cut(s) 694, 1891
ErhI CCWWGG 2 cut(s) 121, 2255
FalI AAGNNNNNCTT 4 cut(s) 524, 556, 547, 579
FaqI GGGAC 1 cut(s) 18
FauI CCCGC 1 cut(s) 330
FbaI TGATCA 2 cut(s) 1006, 1963
FblI GTMKAC 3 cut(s) 22, 1846, 1910
Fnu4HI GCNGC 9 cut(s) 94, 339, 663, 666, 669, 731, 951, 973, 1949
FokI GGATG 4 cut(s) 1028, 1229, 1328, 2203
Fsp4HI GCNGC 9 cut(s) 94, 339, 663, 666, 669, 731, 951, 973, 1949
FspBI CTAG 3 cut(s) 158, 935, 1679
GlaI GCGC 1 cut(s) 834
GluI GCNGC 9 cut(s) 94, 339, 663, 666, 669, 731, 951, 973, 1949
GsaI CCCAGC 1 cut(s) 1107
GsuI CTGGAG 1 cut(s) 940
HaeIII GGCC 1 cut(s) 210
HapII CCGG 2 cut(s) 924, 2264
HgaI GACGC 1 cut(s) 1805
HhaI GCGC 1 cut(s) 835
Hin1I GRCGYC 1 cut(s) 1801
Hin6I GCGC 1 cut(s) 833
HinP1I GCGC 1 cut(s) 833
HincII GTYRAC 4 cut(s) 23, 403, 799, 1038
HindII GTYRAC 4 cut(s) 23, 403, 799, 1038
HindIII AAGCTT 3 cut(s) 249, 1283, 1993
HinfI GANTC 9 cut(s) 42, 224, 481, 497, 746, 1089, 1160, 1507, 2009
HpaI GTTAAC 1 cut(s) 799
HpaII CCGG 2 cut(s) 924, 2264
HphI GGTGA 9 cut(s) 23, 110, 140, 626, 1285, 1577, 1745, 2093, 2254
Hpy99I CGWCG 4 cut(s) 80, 83, 1232, 2151
HpyAV CCTTC 4 cut(s) 125, 717, 1696, 2144
HpyCH4III ACNGT 5 cut(s) 11, 796, 1413, 1714, 2210
HpyCH4IV ACGT 5 cut(s) 81, 1227, 1561, 1801, 2146
HpyF10VI GCNNNNNNNGC 9 cut(s) 207, 388, 443, 659, 668, 739, 832, 992, 1613
HpyF3I CTNAG 6 cut(s) 510, 1725, 1755, 1839, 1851, 1920
HpySE526I ACGT 5 cut(s) 81, 1227, 1561, 1801, 2146
Hsp92I GRCGYC 1 cut(s) 1801
HspAI GCGC 1 cut(s) 833
Ksp22I TGATCA 2 cut(s) 1006, 1963
KspAI GTTAAC 1 cut(s) 799
LguI GCTCTTC 2 cut(s) 851, 1548
LmnI GCTCC 3 cut(s) 42, 623, 2015
Lsp1109I GCAGC 8 cut(s) 105, 325, 652, 674, 742, 937, 959, 1960
LweI GCATC 2 cut(s) 291, 2107
MaeI CTAG 3 cut(s) 158, 935, 1679
MaeII ACGT 5 cut(s) 81, 1227, 1561, 1801, 2146
MaeIII GTNAC 2 cut(s) 11, 1114
MbiI CCGCTC 1 cut(s) 236
MflI RGATCY 3 cut(s) 893, 2159, 2260
MhlI GDGCHC 1 cut(s) 1227
MlyI GAGTC 1 cut(s) 36
MmeI TCCRAC 3 cut(s) 64, 747, 1356
Mph1103I ATGCAT 2 cut(s) 694, 1891
MroXI GAANNNNTTC 1 cut(s) 1245
MslI CAYNNNNRTG 6 cut(s) 123, 126, 608, 1304, 2112, 2168
MspA1I CMGCKG 3 cut(s) 96, 665, 671
MspCI CTTAAG 2 cut(s) 473, 530
MspI CCGG 2 cut(s) 924, 2264
MspR9I CCNGG 1 cut(s) 958
MssI GTTTAAAC 1 cut(s) 2287
Mva1269I GAATGC 1 cut(s) 325
MvaI CCWGG 1 cut(s) 958
MwoI GCNNNNNNNGC 9 cut(s) 207, 388, 443, 659, 668, 739, 832, 992, 1613
NcoI CCATGG 1 cut(s) 121
NlaIV GGNNCC 3 cut(s) 922, 2161, 2262
NmeAIII GCCGAG 1 cut(s) 435
NmuCI GTSAC 2 cut(s) 11, 1114
NsiI ATGCAT 2 cut(s) 694, 1891
NspI RCATGY 2 cut(s) 1530, 1790
NspV TTCGAA 1 cut(s) 1249
OliI CACNNNNGTG 2 cut(s) 123, 1304
PaeI GCATGC 1 cut(s) 1530
PagI TCATGA 2 cut(s) 1503, 1549
PciSI GCTCTTC 2 cut(s) 851, 1548
PctI GAATGC 1 cut(s) 325
PdmI GAANNNNTTC 1 cut(s) 1245
PfeI GAWTC 8 cut(s) 224, 481, 497, 746, 1089, 1160, 1507, 2009
PflFI GACNNNGTC 1 cut(s) 113
PfoI TCCNGGA 1 cut(s) 956
PkrI GCNGC 9 cut(s) 95, 340, 664, 667, 670, 732, 952, 974, 1950
PleI GAGTC 1 cut(s) 36
PmeI GTTTAAAC 1 cut(s) 2287
PpsI GAGTC 1 cut(s) 36
PshAI GACNNNNGTC 1 cut(s) 18
PsiI TTATAA 1 cut(s) 1541
Psp6I CCWGG 1 cut(s) 956
PspFI CCCAGC 1 cut(s) 1103
PspGI CCWGG 1 cut(s) 956
PspN4I GGNNCC 3 cut(s) 922, 2161, 2262
PspPI GGNCC 2 cut(s) 629, 1349
PstI CTGCAG 1 cut(s) 95
PstNI CAGNNNCTG 1 cut(s) 2018
PsuI RGATCY 3 cut(s) 893, 2159, 2260
PsyI GACNNNGTC 1 cut(s) 113
PvuII CAGCTG 1 cut(s) 665
RsaI GTAC 8 cut(s) 84, 242, 909, 1410, 1651, 1883, 1931, 2238
RsaNI GTAC 8 cut(s) 83, 241, 908, 1409, 1650, 1882, 1930, 2237
RseI CAYNNNNRTG 6 cut(s) 123, 126, 608, 1304, 2112, 2168
SalI GTCGAC 1 cut(s) 21
SapI GCTCTTC 2 cut(s) 851, 1548
SatI GCNGC 9 cut(s) 94, 339, 663, 666, 669, 731, 951, 973, 1949
Sau96I GGNCC 2 cut(s) 629, 1349
SchI GAGTC 1 cut(s) 36
ScrFI CCNGG 1 cut(s) 958
SduI GDGCHC 1 cut(s) 1227
SfaNI GCATC 2 cut(s) 291, 2107
SfcI CTRYAG 1 cut(s) 91
SfuI TTCGAA 1 cut(s) 1249
SinI GGWCC 2 cut(s) 629, 1349
SmiMI CAYNNNNRTG 6 cut(s) 123, 126, 608, 1304, 2112, 2168
SmlI CTYRAG 4 cut(s) 473, 530, 828, 862
SmoI CTYRAG 4 cut(s) 473, 530, 828, 862
SphI GCATGC 1 cut(s) 1530
SsiI CCGC 7 cut(s) 96, 184, 234, 323, 669, 1296, 1607
SspI AATATT 3 cut(s) 216, 525, 1460
SspMI CTAG 3 cut(s) 158, 935, 1679
StyD4I CCNGG 1 cut(s) 956
StyI CCWWGG 2 cut(s) 121, 2255
TaaI ACNGT 5 cut(s) 11, 796, 1413, 1714, 2210
TaiI ACGT 5 cut(s) 84, 1230, 1564, 1804, 2149
TaqI TCGA 3 cut(s) 22, 222, 1249
TatI WGTACW 6 cut(s) 240, 907, 1408, 1881, 1929, 2236
TauI GCSGC 1 cut(s) 671
TfiI GAWTC 8 cut(s) 224, 481, 497, 746, 1089, 1160, 1507, 2009
TscAI CASTG 3 cut(s) 1016, 1283, 1418
TseFI GTSAC 2 cut(s) 11, 1114
TseI GCWGC 8 cut(s) 93, 338, 662, 665, 730, 950, 972, 1948
Tsp45I GTSAC 2 cut(s) 11, 1114
TspDTI ATGAA 8 cut(s) 418, 663, 1065, 1377, 1520, 1538, 1566, 2229
TspGWI ACGGA 3 cut(s) 377, 1082, 1928
TspRI CASTG 3 cut(s) 1016, 1283, 1418
Tth111I GACNNNGTC 1 cut(s) 113
Vha464I CTTAAG 2 cut(s) 473, 530
VneI GTGCAC 1 cut(s) 1223
VpaK11BI GGWCC 2 cut(s) 629, 1349
XagI CCTNNNNNAGG 1 cut(s) 2010
XapI RAATTY 2 cut(s) 1056, 1674
XbaI TCTAGA 1 cut(s) 1678
XceI RCATGY 2 cut(s) 1530, 1790
XmiI GTMKAC 3 cut(s) 22, 1846, 1910
XmnI GAANNNNTTC 1 cut(s) 1245
XspI CTAG 3 cut(s) 158, 935, 1679
ZraI GACGTC 1 cut(s) 1802
Zsp2I ATGCAT 2 cut(s) 694, 1891
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.