DNA repair helicase
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| Helicase_C_3 | PF13625 | 47 - 170 | 7.9e-33 | Helicase conserved C-terminal domain |
| ResIII | PF04851 | 262 - 419 | 3.2e-18 | Type III restriction enzyme, res subunit |
| ERCC3_RAD25_C | PF16203 | 444 - 690 | 2e-115 | ERCC3/RAD25/XPB C-terminal helicase |
| Helicase_C | PF00271 | 494 - 596 | 3.3e-13 | Helicase conserved C-terminal domain |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 1753 |
| AccB1I | GGYRCC | 1 cut(s) | 2129 |
| AccI | GTMKAC | 2 cut(s) | 1795, 1859 |
| AciI | CCGC | 7 cut(s) | 45, 70, 224, 272, 287, 867, 1341 |
| AclI | AACGTT | 1 cut(s) | 682 |
| AclWI | GGATC | 6 cut(s) | 647, 1812, 1972, 2116, 2213, 2226 |
| AcoI | YGGCCR | 1 cut(s) | 157 |
| AcsI | RAATTY | 3 cut(s) | 1005, 1211, 1623 |
| AcuI | CTGAAG | 4 cut(s) | 488, 642, 1389, 2069 |
| AcvI | CACGTG | 1 cut(s) | 19 |
| AcyI | GRCGYC | 2 cut(s) | 1750, 2130 |
| AfaI | GTAC | 8 cut(s) | 191, 465, 858, 1359, 1832, 1880, 2161, 2206 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 237, 1675, 1961 |
| AflII | CTTAAG | 2 cut(s) | 422, 479 |
| AflIII | ACRYGT | 1 cut(s) | 16 |
| AjiI | CACGTC | 1 cut(s) | 2096 |
| AjnI | CCWGG | 3 cut(s) | 168, 1401, 2213 |
| AleI | CACNNNNGTG | 3 cut(s) | 17, 1253, 1512 |
| Alw21I | GWGCWC | 1 cut(s) | 1509 |
| Alw26I | GTCTC | 4 cut(s) | 87, 298, 2045, 2224 |
| AlwI | GGATC | 6 cut(s) | 647, 1812, 1972, 2116, 2213, 2226 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1967 |
| AoxI | GGCC | 3 cut(s) | 134, 157, 301 |
| ApeKI | GCWGC | 3 cut(s) | 611, 899, 921 |
| ApoI | RAATTY | 3 cut(s) | 1005, 1211, 1623 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1194 |
| AspLEI | GCGC | 3 cut(s) | 784, 2132, 2194 |
| AspS9I | GGNCC | 4 cut(s) | 521, 578, 1101, 1298 |
| AsuHPI | GGTGA | 7 cut(s) | 89, 575, 1267, 1526, 1694, 1973, 2042 |
| AsuII | TTCGAA | 1 cut(s) | 1198 |
| AvaII | GGWCC | 4 cut(s) | 521, 578, 1101, 1298 |
| AxyI | CCTNAGG | 2 cut(s) | 1704, 1800 |
| BamHI | GGATCC | 1 cut(s) | 2218 |
| BanI | GGYRCC | 1 cut(s) | 2129 |
| BanII | GRGCYC | 1 cut(s) | 1242 |
| BauI | CACGAG | 2 cut(s) | 254, 416 |
| BbrPI | CACGTG | 1 cut(s) | 19 |
| BbsI | GAAGAC | 2 cut(s) | 476, 2070 |
| Bbv12I | GWGCWC | 1 cut(s) | 1509 |
| BbvI | GCAGC | 3 cut(s) | 623, 908, 911 |
| BccI | CCATC | 3 cut(s) | 149, 553, 2134 |
| BceAI | ACGGC | 1 cut(s) | 49 |
| BciT130I | CCWGG | 3 cut(s) | 170, 1403, 2215 |
| BclI | TGATCA | 2 cut(s) | 955, 1912 |
| BcoDI | GTCTC | 4 cut(s) | 87, 298, 2045, 2224 |
| BfaI | CTAG | 4 cut(s) | 107, 582, 884, 2057 |
| BfmI | CTRYAG | 1 cut(s) | 897 |
| BfoI | RGCGCY | 1 cut(s) | 2133 |
| BfrI | CTTAAG | 2 cut(s) | 422, 479 |
| BglII | AGATCT | 3 cut(s) | 842, 1279, 1725 |
| BisI | GCNGC | 4 cut(s) | 288, 612, 900, 922 |
| BlsI | GCNGC | 4 cut(s) | 289, 613, 901, 923 |
| BmcAI | AGTACT | 2 cut(s) | 2161, 2206 |
| Bme1390I | CCNGG | 3 cut(s) | 170, 1403, 2215 |
| Bme18I | GGWCC | 4 cut(s) | 521, 578, 1101, 1298 |
| BmgBI | CACGTC | 1 cut(s) | 2096 |
| BmgT120I | GGNCC | 4 cut(s) | 521, 578, 1101, 1298 |
| BmiI | GGNNCC | 5 cut(s) | 228, 522, 1300, 2131, 2220 |
| BmrFI | CCNGG | 3 cut(s) | 170, 1403, 2215 |
| BmsI | GCATC | 1 cut(s) | 240 |
| BpiI | GAAGAC | 2 cut(s) | 476, 2070 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1788 |
| Bpu14I | TTCGAA | 1 cut(s) | 1198 |
| BpuEI | CTTGAG | 2 cut(s) | 762, 832 |
| Bsa29I | ATCGAT | 1 cut(s) | 433 |
| BsaAI | YACGTR | 1 cut(s) | 19 |
| BsaHI | GRCGYC | 2 cut(s) | 1750, 2130 |
| BsaJI | CCNNGG | 7 cut(s) | 73, 1042, 1510, 2133, 2213, 2214, 2254 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 237, 1675, 1961 |
| Bse1I | ACTGG | 4 cut(s) | 612, 1257, 1325, 2004 |
| Bse21I | CCTNAGG | 2 cut(s) | 1704, 1800 |
| Bse3DI | GCAATG | 5 cut(s) | 213, 219, 473, 1225, 1572 |
| BseBI | CCWGG | 3 cut(s) | 170, 1403, 2215 |
| BseCI | ATCGAT | 1 cut(s) | 433 |
| BseDI | CCNNGG | 7 cut(s) | 73, 1042, 1510, 2133, 2213, 2214, 2254 |
| BseGI | GGATG | 4 cut(s) | 255, 1171, 1270, 2145 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 237, 1675, 1961 |
| BseMI | GCAATG | 5 cut(s) | 213, 219, 473, 1225, 1572 |
| BseMII | CTCAG | 6 cut(s) | 309, 507, 1602, 1695, 1814, 1883 |
| BseNI | ACTGG | 4 cut(s) | 612, 1257, 1325, 2004 |
| BseRI | GAGGAG | 2 cut(s) | 734, 1841 |
| BseXI | GCAGC | 3 cut(s) | 623, 908, 911 |
| BseYI | CCCAGC | 1 cut(s) | 1052 |
| Bsh1285I | CGRYCG | 2 cut(s) | 49, 794 |
| BshFI | GGCC | 3 cut(s) | 136, 159, 303 |
| BshNI | GGYRCC | 1 cut(s) | 2129 |
| BshVI | ATCGAT | 1 cut(s) | 433 |
| BsiEI | CGRYCG | 2 cut(s) | 49, 794 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1509 |
| BsiSI | CCGG | 2 cut(s) | 160, 2222 |
| BslFI | GGGAC | 3 cut(s) | 1284, 1365, 1664 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 237, 1675, 1961 |
| BsmAI | GTCTC | 4 cut(s) | 87, 298, 2045, 2224 |
| BsmFI | GGGAC | 3 cut(s) | 1284, 1365, 1664 |
| BsmI | GAATGC | 1 cut(s) | 274 |
| BsnI | GGCC | 3 cut(s) | 136, 159, 303 |
| Bsp119I | TTCGAA | 1 cut(s) | 1198 |
| Bsp1286I | GDGCHC | 2 cut(s) | 1242, 1509 |
| Bsp1407I | TGTACA | 2 cut(s) | 189, 1357 |
| Bsp19I | CCATGG | 2 cut(s) | 73, 2254 |
| BspACI | CCGC | 7 cut(s) | 45, 70, 224, 272, 287, 867, 1341 |
| BspANI | GGCC | 3 cut(s) | 136, 159, 303 |
| BspCNI | CTCAG | 6 cut(s) | 308, 508, 1603, 1696, 1813, 1882 |
| BspDI | ATCGAT | 1 cut(s) | 433 |
| BspHI | TCATGA | 1 cut(s) | 1498 |
| BspLI | GGNNCC | 5 cut(s) | 228, 522, 1300, 2131, 2220 |
| BspMAI | CTGCAG | 1 cut(s) | 901 |
| BspPI | GGATC | 6 cut(s) | 647, 1812, 1972, 2116, 2213, 2226 |
| BspQI | GCTCTTC | 2 cut(s) | 800, 1497 |
| BspT104I | TTCGAA | 1 cut(s) | 1198 |
| BspT107I | GGYRCC | 1 cut(s) | 2129 |
| BspTI | CTTAAG | 2 cut(s) | 422, 479 |
| BsrDI | GCAATG | 5 cut(s) | 213, 219, 473, 1225, 1572 |
| BsrGI | TGTACA | 2 cut(s) | 189, 1357 |
| BsrI | ACTGG | 4 cut(s) | 612, 1257, 1325, 2004 |
| BssECI | CCNNGG | 7 cut(s) | 73, 1042, 1510, 2133, 2213, 2214, 2254 |
| BssNI | GRCGYC | 2 cut(s) | 1750, 2130 |
| BssSI | CACGAG | 2 cut(s) | 254, 416 |
| BssT1I | CCWWGG | 3 cut(s) | 73, 2133, 2254 |
| Bst2BI | CACGAG | 2 cut(s) | 254, 416 |
| Bst2UI | CCWGG | 3 cut(s) | 170, 1403, 2215 |
| Bst4CI | ACNGT | 7 cut(s) | 745, 795, 1362, 1494, 1511, 1663, 1985 |
| Bst6I | CTCTTC | 4 cut(s) | 800, 1409, 1497, 2222 |
| BstACI | GRCGYC | 2 cut(s) | 1750, 2130 |
| BstAFI | CTTAAG | 2 cut(s) | 422, 479 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 941 |
| BstAUI | TGTACA | 2 cut(s) | 189, 1357 |
| BstBAI | YACGTR | 1 cut(s) | 19 |
| BstBI | TTCGAA | 1 cut(s) | 1198 |
| BstC8I | GCNNGC | 7 cut(s) | 239, 689, 1232, 1440, 1477, 1898, 1946 |
| BstDSI | CCRYGG | 4 cut(s) | 73, 1042, 1510, 2254 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 1673, 1959 |
| BstF5I | GGATG | 4 cut(s) | 255, 1171, 1270, 2145 |
| BstH2I | RGCGCY | 1 cut(s) | 2133 |
| BstHHI | GCGC | 3 cut(s) | 784, 2132, 2194 |
| BstMAI | GTCTC | 4 cut(s) | 87, 298, 2045, 2224 |
| BstMCI | CGRYCG | 2 cut(s) | 49, 794 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 42, 608, 617, 781, 941 |
| BstNI | CCWGG | 3 cut(s) | 170, 1403, 2215 |
| BstNSI | RCATGY | 3 cut(s) | 1178, 1479, 1739 |
| BstSCI | CCNGG | 3 cut(s) | 168, 1401, 2213 |
| BstSFI | CTRYAG | 1 cut(s) | 897 |
| BstV1I | GCAGC | 3 cut(s) | 623, 908, 911 |
| BstV2I | GAAGAC | 2 cut(s) | 476, 2070 |
| BstX2I | RGATCY | 6 cut(s) | 842, 1279, 1725, 1964, 2108, 2218 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 2140 |
| BstYI | RGATCY | 6 cut(s) | 842, 1279, 1725, 1964, 2108, 2218 |
| Bsu15I | ATCGAT | 1 cut(s) | 433 |
| Bsu36I | CCTNAGG | 2 cut(s) | 1704, 1800 |
| BsuRI | GGCC | 3 cut(s) | 136, 159, 303 |
| BsuTUI | ATCGAT | 1 cut(s) | 433 |
| BtgI | CCRYGG | 4 cut(s) | 73, 1042, 1510, 2254 |
| BtrI | CACGTC | 1 cut(s) | 2096 |
| BtsCI | GGATG | 4 cut(s) | 255, 1171, 1270, 2145 |
| BtsI | GCAGTG | 1 cut(s) | 1242 |
| BtsIMutI | CAGTG | 4 cut(s) | 965, 1242, 1250, 1367 |
| Cac8I | GCNNGC | 7 cut(s) | 239, 689, 1232, 1440, 1477, 1898, 1946 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1967 |
| CciI | TCATGA | 1 cut(s) | 1498 |
| CfoI | GCGC | 3 cut(s) | 784, 2132, 2194 |
| Cfr13I | GGNCC | 4 cut(s) | 521, 578, 1101, 1298 |
| ClaI | ATCGAT | 1 cut(s) | 433 |
| CseI | GACGC | 1 cut(s) | 1754 |
| Csp6I | GTAC | 8 cut(s) | 190, 464, 857, 1358, 1831, 1879, 2160, 2205 |
| CviQI | GTAC | 8 cut(s) | 190, 464, 857, 1358, 1831, 1879, 2160, 2205 |
| DinI | GGCGCC | 1 cut(s) | 2131 |
| DraI | TTTAAA | 1 cut(s) | 2245 |
| EaeI | YGGCCR | 1 cut(s) | 157 |
| Eam1104I | CTCTTC | 4 cut(s) | 800, 1409, 1497, 2222 |
| EarI | CTCTTC | 4 cut(s) | 800, 1409, 1497, 2222 |
| EciI | GGCGGA | 2 cut(s) | 59, 1356 |
| Eco130I | CCWWGG | 3 cut(s) | 73, 2133, 2254 |
| Eco24I | GRGCYC | 1 cut(s) | 1242 |
| Eco47I | GGWCC | 4 cut(s) | 521, 578, 1101, 1298 |
| Eco57I | CTGAAG | 4 cut(s) | 488, 642, 1389, 2069 |
| Eco72I | CACGTG | 1 cut(s) | 19 |
| Eco81I | CCTNAGG | 2 cut(s) | 1704, 1800 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 1673, 1959 |
| EcoO109I | RGGNCCY | 1 cut(s) | 521 |
| EcoRII | CCWGG | 3 cut(s) | 168, 1401, 2213 |
| EcoT14I | CCWWGG | 3 cut(s) | 73, 2133, 2254 |
| EcoT22I | ATGCAT | 1 cut(s) | 643 |
| EcoT38I | GRGCYC | 1 cut(s) | 1242 |
| EgeI | GGCGCC | 1 cut(s) | 2131 |
| EheI | GGCGCC | 1 cut(s) | 2131 |
| ErhI | CCWWGG | 3 cut(s) | 73, 2133, 2254 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 473, 505, 496, 528 |
| FaqI | GGGAC | 3 cut(s) | 1284, 1365, 1664 |
| FauI | CCCGC | 1 cut(s) | 279 |
| FbaI | TGATCA | 2 cut(s) | 955, 1912 |
| FblI | GTMKAC | 2 cut(s) | 1795, 1859 |
| Fnu4HI | GCNGC | 4 cut(s) | 288, 612, 900, 922 |
| FokI | GGATG | 4 cut(s) | 262, 1178, 1277, 2152 |
| FriOI | GRGCYC | 1 cut(s) | 1242 |
| Fsp4HI | GCNGC | 4 cut(s) | 288, 612, 900, 922 |
| FspBI | CTAG | 4 cut(s) | 107, 582, 884, 2057 |
| GlaI | GCGC | 3 cut(s) | 783, 2131, 2193 |
| GluI | GCNGC | 4 cut(s) | 288, 612, 900, 922 |
| GsaI | CCCAGC | 1 cut(s) | 1056 |
| HaeII | RGCGCY | 1 cut(s) | 2133 |
| HaeIII | GGCC | 3 cut(s) | 136, 159, 303 |
| HapII | CCGG | 2 cut(s) | 160, 2222 |
| HgaI | GACGC | 1 cut(s) | 1754 |
| HhaI | GCGC | 3 cut(s) | 784, 2132, 2194 |
| Hin1I | GRCGYC | 2 cut(s) | 1750, 2130 |
| Hin6I | GCGC | 3 cut(s) | 782, 2130, 2192 |
| HinP1I | GCGC | 3 cut(s) | 782, 2130, 2192 |
| HincII | GTYRAC | 3 cut(s) | 748, 987, 1860 |
| HindII | GTYRAC | 3 cut(s) | 748, 987, 1860 |
| HindIII | AAGCTT | 4 cut(s) | 198, 1232, 1632, 1942 |
| HinfI | GANTC | 8 cut(s) | 430, 446, 500, 695, 1038, 1109, 1456, 1958 |
| HpaI | GTTAAC | 1 cut(s) | 748 |
| HpaII | CCGG | 2 cut(s) | 160, 2222 |
| HphI | GGTGA | 7 cut(s) | 89, 575, 1267, 1526, 1694, 1973, 2042 |
| Hpy166II | GTNNAC | 6 cut(s) | 748, 987, 1657, 1796, 1860, 2146 |
| Hpy8I | GTNNAC | 6 cut(s) | 748, 987, 1657, 1796, 1860, 2146 |
| Hpy99I | CGWCG | 1 cut(s) | 2100 |
| HpyAV | CCTTC | 3 cut(s) | 74, 666, 2093 |
| HpyCH4III | ACNGT | 7 cut(s) | 745, 795, 1362, 1494, 1511, 1663, 1985 |
| HpyCH4IV | ACGT | 6 cut(s) | 18, 682, 1653, 1750, 1774, 2095 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 42, 608, 617, 781, 941 |
| HpySE526I | ACGT | 6 cut(s) | 18, 682, 1653, 1750, 1774, 2095 |
| Hsp92I | GRCGYC | 2 cut(s) | 1750, 2130 |
| HspAI | GCGC | 3 cut(s) | 782, 2130, 2192 |
| KasI | GGCGCC | 1 cut(s) | 2129 |
| Ksp22I | TGATCA | 2 cut(s) | 955, 1912 |
| KspAI | GTTAAC | 1 cut(s) | 748 |
| LguI | GCTCTTC | 2 cut(s) | 800, 1497 |
| LmnI | GCTCC | 2 cut(s) | 360, 572 |
| Lsp1109I | GCAGC | 3 cut(s) | 623, 908, 911 |
| LweI | GCATC | 1 cut(s) | 240 |
| MaeI | CTAG | 4 cut(s) | 107, 582, 884, 2057 |
| MaeII | ACGT | 6 cut(s) | 18, 682, 1653, 1750, 1774, 2095 |
| MaeIII | GTNAC | 2 cut(s) | 1063, 1682 |
| MflI | RGATCY | 6 cut(s) | 842, 1279, 1725, 1964, 2108, 2218 |
| MhlI | GDGCHC | 2 cut(s) | 1242, 1509 |
| Mly113I | GGCGCC | 1 cut(s) | 2130 |
| MlyI | GAGTC | 2 cut(s) | 455, 1047 |
| MmeI | TCCRAC | 2 cut(s) | 696, 1305 |
| Mph1103I | ATGCAT | 1 cut(s) | 643 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1194 |
| MslI | CAYNNNNRTG | 5 cut(s) | 17, 557, 1253, 1512, 2181 |
| MspA1I | CMGCKG | 2 cut(s) | 45, 614 |
| MspCI | CTTAAG | 2 cut(s) | 422, 479 |
| MspI | CCGG | 2 cut(s) | 160, 2222 |
| MspR9I | CCNGG | 3 cut(s) | 170, 1403, 2215 |
| MssI | GTTTAAAC | 1 cut(s) | 2245 |
| Mva1269I | GAATGC | 1 cut(s) | 274 |
| MvaI | CCWGG | 3 cut(s) | 170, 1403, 2215 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 42, 608, 617, 781, 941 |
| NarI | GGCGCC | 1 cut(s) | 2130 |
| NcoI | CCATGG | 2 cut(s) | 73, 2254 |
| NlaIV | GGNNCC | 5 cut(s) | 228, 522, 1300, 2131, 2220 |
| NmeAIII | GCCGAG | 1 cut(s) | 161 |
| NmuCI | GTSAC | 2 cut(s) | 1063, 1682 |
| NsiI | ATGCAT | 1 cut(s) | 643 |
| NspI | RCATGY | 3 cut(s) | 1178, 1479, 1739 |
| NspV | TTCGAA | 1 cut(s) | 1198 |
| OliI | CACNNNNGTG | 3 cut(s) | 17, 1253, 1512 |
| PaeI | GCATGC | 1 cut(s) | 1479 |
| PagI | TCATGA | 1 cut(s) | 1498 |
| PasI | CCCWGGG | 1 cut(s) | 2214 |
| PciSI | GCTCTTC | 2 cut(s) | 800, 1497 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 24, 1659 |
| PctI | GAATGC | 1 cut(s) | 274 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1194 |
| PfeI | GAWTC | 6 cut(s) | 430, 500, 695, 1109, 1456, 1958 |
| PflFI | GACNNNGTC | 1 cut(s) | 65 |
| PfoI | TCCNGGA | 2 cut(s) | 168, 1401 |
| PkrI | GCNGC | 4 cut(s) | 289, 613, 901, 923 |
| PleI | GAGTC | 2 cut(s) | 454, 1046 |
| PluTI | GGCGCC | 1 cut(s) | 2133 |
| PmaCI | CACGTG | 1 cut(s) | 19 |
| PmeI | GTTTAAAC | 1 cut(s) | 2245 |
| PmlI | CACGTG | 1 cut(s) | 19 |
| PpsI | GAGTC | 2 cut(s) | 454, 1046 |
| Ppu21I | YACGTR | 1 cut(s) | 19 |
| PpuMI | RGGWCCY | 1 cut(s) | 521 |
| Psp1406I | AACGTT | 1 cut(s) | 682 |
| Psp5II | RGGWCCY | 1 cut(s) | 521 |
| Psp6I | CCWGG | 3 cut(s) | 168, 1401, 2213 |
| PspCI | CACGTG | 1 cut(s) | 19 |
| PspFI | CCCAGC | 1 cut(s) | 1052 |
| PspGI | CCWGG | 3 cut(s) | 168, 1401, 2213 |
| PspN4I | GGNNCC | 5 cut(s) | 228, 522, 1300, 2131, 2220 |
| PspPI | GGNCC | 4 cut(s) | 521, 578, 1101, 1298 |
| PspPPI | RGGWCCY | 1 cut(s) | 521 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1823, 1855 |
| PstI | CTGCAG | 1 cut(s) | 901 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1967 |
| PsuI | RGATCY | 6 cut(s) | 842, 1279, 1725, 1964, 2108, 2218 |
| PsyI | GACNNNGTC | 1 cut(s) | 65 |
| PvuII | CAGCTG | 1 cut(s) | 614 |
| RsaI | GTAC | 8 cut(s) | 191, 465, 858, 1359, 1832, 1880, 2161, 2206 |
| RsaNI | GTAC | 8 cut(s) | 190, 464, 857, 1358, 1831, 1879, 2160, 2205 |
| RseI | CAYNNNNRTG | 5 cut(s) | 17, 557, 1253, 1512, 2181 |
| SalI | GTCGAC | 1 cut(s) | 1858 |
| SapI | GCTCTTC | 2 cut(s) | 800, 1497 |
| SatI | GCNGC | 4 cut(s) | 288, 612, 900, 922 |
| Sau96I | GGNCC | 4 cut(s) | 521, 578, 1101, 1298 |
| ScaI | AGTACT | 2 cut(s) | 2161, 2206 |
| SchI | GAGTC | 2 cut(s) | 455, 1047 |
| ScrFI | CCNGG | 3 cut(s) | 170, 1403, 2215 |
| SduI | GDGCHC | 2 cut(s) | 1242, 1509 |
| SfaNI | GCATC | 1 cut(s) | 240 |
| SfcI | CTRYAG | 1 cut(s) | 897 |
| SfoI | GGCGCC | 1 cut(s) | 2131 |
| SfuI | TTCGAA | 1 cut(s) | 1198 |
| SinI | GGWCC | 4 cut(s) | 521, 578, 1101, 1298 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 17, 557, 1253, 1512, 2181 |
| SmlI | CTYRAG | 4 cut(s) | 422, 479, 777, 811 |
| SmoI | CTYRAG | 4 cut(s) | 422, 479, 777, 811 |
| SphI | GCATGC | 1 cut(s) | 1479 |
| SsiI | CCGC | 7 cut(s) | 45, 70, 224, 272, 287, 867, 1341 |
| SspDI | GGCGCC | 1 cut(s) | 2129 |
| SspI | AATATT | 1 cut(s) | 1409 |
| SspMI | CTAG | 4 cut(s) | 107, 582, 884, 2057 |
| StyD4I | CCNGG | 3 cut(s) | 168, 1401, 2213 |
| StyI | CCWWGG | 3 cut(s) | 73, 2133, 2254 |
| TaaI | ACNGT | 7 cut(s) | 745, 795, 1362, 1494, 1511, 1663, 1985 |
| TaiI | ACGT | 6 cut(s) | 21, 685, 1656, 1753, 1777, 2098 |
| TaqI | TCGA | 5 cut(s) | 433, 444, 907, 1198, 1859 |
| TatI | WGTACW | 7 cut(s) | 189, 856, 1357, 1830, 1878, 2159, 2204 |
| TauI | GCSGC | 1 cut(s) | 290 |
| TfiI | GAWTC | 6 cut(s) | 430, 500, 695, 1109, 1456, 1958 |
| TscAI | CASTG | 4 cut(s) | 965, 1249, 1257, 1367 |
| TseFI | GTSAC | 2 cut(s) | 1063, 1682 |
| TseI | GCWGC | 3 cut(s) | 611, 899, 921 |
| Tsp45I | GTSAC | 2 cut(s) | 1063, 1682 |
| TspDTI | ATGAA | 8 cut(s) | 367, 549, 612, 1326, 1469, 1487, 1515, 1583 |
| TspGWI | ACGGA | 2 cut(s) | 940, 1031 |
| TspRI | CASTG | 4 cut(s) | 965, 1249, 1257, 1367 |
| Tth111I | GACNNNGTC | 1 cut(s) | 65 |
| Vha464I | CTTAAG | 2 cut(s) | 422, 479 |
| VpaK11BI | GGWCC | 4 cut(s) | 521, 578, 1101, 1298 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 1673, 1959 |
| XapI | RAATTY | 3 cut(s) | 1005, 1211, 1623 |
| XceI | RCATGY | 3 cut(s) | 1178, 1479, 1739 |
| XmiI | GTMKAC | 2 cut(s) | 1795, 1859 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1194 |
| XspI | CTAG | 4 cut(s) | 107, 582, 884, 2057 |
| ZraI | GACGTC | 1 cut(s) | 1751 |
| ZrmI | AGTACT | 2 cut(s) | 2161, 2206 |
| Zsp2I | ATGCAT | 1 cut(s) | 643 |