Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0001033

Physical Location & Seq

Genomic Coordinates Reverse (-)

35662266 .. 35673040

Gene Structure 10775 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00312590.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2391 bp

ATGGGAGGTGATTTTGCGTCGAATGGTGGCCGGATGGTGGAGAACTTGGCCGAGGAGCTCGCTCTGTTTTTTTTTGTTTCGGGCGAAGGTGAGAAAGGCCGGCGCAATAAAAAACTCAAAGTCTCTGCCAAGGAAAGCACACACGTGGAAAACGATGACGGCTATTACAGCGGTCGTGAAGAAGATGACAATGTCCGCCATGGTGAAGGGAAAACGAGAGACTTTACCAAACTAGAATTGAAGCCTGACCATCCTAATCGGCCTTTATGGGCTTGTCCAGATGGCCGGATATTCCTGGAAACTTTCTCGGCTTTGTACAAACAAGCTTATGATTTCCTCATTGCCATTGCGGAGCCCGTTTGCAGGCAAGAGTGGATGCACGAGTATAACTTGACCCCGCATTCTTTATATGCGGCTGTCTCAGTTGGCCTTGAAACCGAAACAATTATTTCTGTTCTAAATAAGCTGTCAAAGACCAAGCTCCCCAAAGAGATTATTGATTTCATACATGGTTCTACTGCTAATTATGGCAAAGTGAAGCTCGTGCTTAAGAAGAATCGATATTTTGTCGAGTCGCCATATCCAGAGGCAGGATTTTATGCCTCCCCTAATTACCTTTTTCAGTTTACATTAGTGATGCAGATTTTTGGGGTTTATTTAAATGCGGTTTATAATCTTTTGCAGGTACTGAAGACATTGCTTAAGGACAACTTTATTGCAGAATCAAGGATTACTTCTGAGGGTCCTCATGGAAATGAAGATTTTACAGTTAGCACAACGATGGGTGAAATTGGAGCTGGTCCTAGTGATTTGCTAAATGAAGAAGAACTGGCAGCTGTGGCTGAAGAAAAAGAAACTCATGCATTTGAAATTGATCCTTCAAAGGTTGAGAATGTTAAGCAACGTTGCTTGCCAGATTCTCTGAACTATCCTATGTTGGAGGAGTATGACTTCAAAAATGATACAGTTAACCCTGATCTAGATATGGAACTAAAGCCTCAAGCACAACCCCGACCGTATCAAGAGAAGAGCTTGAGTAAAATGTTTGGGAATGGGAGGGCAAGATCTGGCATTATTGTACTGCCTTGCGGTGCTGGAAAATCTCTAGTTGGTGTTTCTGCAGCCTCTCGAATAAGAAAGAGCTGCCTTTGTTTAGCAACAAATGCTGTTTCCGTTGATCAGTGGGCTTTTCAGTTTAAGTTGTGGTCAACTATCCGAGATGACCAAATTTGTCGTTTCACATCTGATAGTAAAGAAAGAATCCGTGGAAATGCTGGGGTGGTTGTGACAACATATAACATGATTGGTTTTGGTGGAAAGAGGTCCGAAGAATCTGAAAAGATTATTGAAGAAATAAGAAACAGAGAATGGGGATTGCTTCTTATGGATGAGGTGCATGTTGTTCCTGCTGAAATGTTTCGAAATGTCATCAAATTTACTAAATCACATTGCAAGCTTGGGCTCACTGCCACACTGGTGAGAGAGGATGAGAAGATCACAGATCTGAACTTCCTTATTGGTCCCAAATTGTATGAAGCAAACTGGTTGGATTTGGTTAAAGGCGGATTTATTGCTAATGTACAGTGTGCTGAAGTATGGTGTCCTATGACAAAAGAGTTTTTCCTGGAATATTTGAAGAGGGAAAACTCAAAGAAAAAGCAGGCACTTTATGTAATGAATCCAAATAAGTTTAGGGCATGCGAGTTTCTCATACGGTTTCATGAAGAGCACCGTGGTGATAAAATTATTGTATTTGCCGATAATCTATTTGCACTTACAGAGTATGCAATGAAACTTCGGAAACCTATGATCTATGGTGCTACCAGCCATGCTGAGAGGACAAAAATTCTACAAGCTTTCAAAACAAGTCGGGACGTAAACACCGTATTCCTGTCTAAGGTGGGTGATAATTCCATAGACATTCCTGAGGCAAATGTTATTATTCAGATCTCATCACATGCTGGTTCAAGACGTCAAGAAGCACAGCGTCTTGGACGTATTTTGAGGGCTAAGGGTAGACCTCAGGATCGGATTGCAGGAGGTAAAGAGGAGTACAATGCATTCTTTTATTCTCTTGTGTCGACTGATACTCAGGAGATGTACTTTTCAACTAAACGCCAGCAGTTTTTGATTGATCAAGGTTATAGTTTTAAGGTTATTACAAGCTTGCCCCCTCCTGAATCAGGATCTGATTTGAGCTATCACCGTCTTGATGACCAACTGGCACTACTTTCAAAGGTGTTGAGTGTTGGTGATGATAAAGTTGGCTTAGAGACACTAGAAGAAGACCCTGATGAGATTGCCCTTCAGAAAGCACGTCGCTCAACAGGATCCATGAGTTTGATGTCAGGCGCCAAGGGGATGGTTTACATGGAATATAGGTATGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0008150 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0010224 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0050896

KEGG Pathways

Metabolic & Signaling

ko03022 ko03420 map03022 map03420

Pfam Domains

Protein Families

ERCC3_RAD25_C Helicase_C_3 ResIII

Protein Analysis

796

Amino Acids

90.17

Weight (kDa)

6.2

Isoelectric Point (pI)

35.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Helicase_C_3	PF13625	89 - 196	8.2e-29	Helicase conserved C-terminal domain
ResIII	PF04851	336 - 493	1.8e-18	Type III restriction enzyme, res subunit
ERCC3_RAD25_C	PF16203	518 - 764	2.3e-115	ERCC3/RAD25/XPB C-terminal helicase
Helicase_C	PF00271	568 - 670	3.5e-13	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0001033)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G41360 AT5G41360 AT5G41370
fragaria_vesca	FvH4_5g02840 FvH4_5g02840 FvH4_5g02840 FvH4_5g02840 FvH4_5g02840 FvH4_5g19420 FvH4_5g19430 FvH4_7g08220 FvH4_7g08220
malus_domestica	MD06G1087900.v1.1
prunus_persica	Prupe.5G104000_v2.0.a1
pyrus_communis	pycom06g08400
rosa_chinensis	RchiOBHm_Chr1g0341611 RchiOBHm_Chr1g0341621 RchiOBHm_Chr1g0341861 RchiOBHm_Chr7g0197061 RchiOBHm_Chr7g0203931
rosa_laevigata	RLG00000003497 RLG00000003962 RLG00000029105 RLG00000029132
rosa_multiflora	Rmu_co8328521.1_g000001 Rmu_co8329503.1_g000001 Rmu_sc0002302.1_g000034 Rmu_sc0002322.1_g000070 Rmu_sc0003411.1_g000004 Rmu_sc0013871.1_g000001 Rmu_sc0032022.1_g000001
rosa_roxburghii	Rroxscaffold_3G00258690 Rroxscaffold_4G00312270 Rroxscaffold_4G00312590
rosa_rugosa	Rorug01G0154800.1 Rorug07G0037400 Rorug07G0079500
rosa_samantha	Rh1AG168500 Rh1AG170500 Rh1BG136800 Rh1BG138300 Rh1BG138400 Rh1CG156100 Rh1CG158700 Rh1DG170300 Rh1DG170600 Rh1DG170700 Rh7AG162300 Rh7AG209400 Rh7AG209500 Rh7BG164400 Rh7BG208200 Rh7BG208300 Rh7CG170500 Rh7CG221900 Rh7DG163800 Rh7DG216500
rosa_wichuraiana	Rw1G014160 Rw1G014180 Rw7G014170 Rw7G018250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	672
AatII	GACGTC	1 cut(s)	1975
Acc36I	ACCTGC	1 cut(s)	673
AccB1I	GGYRCC	1 cut(s)	2351
AccI	GTMKAC	2 cut(s)	2017, 2081
AciI	CCGC	8 cut(s)	171, 196, 350, 398, 413, 665, 1089, 1563
AclI	AACGTT	1 cut(s)	904
AclWI	GGATC	5 cut(s)	869, 2034, 2194, 2325, 2338
AcoI	YGGCCR	3 cut(s)	28, 48, 283
AcsI	RAATTY	3 cut(s)	1227, 1433, 1845
AcuI	CTGAAG	4 cut(s)	710, 864, 1611, 2291
AcvI	CACGTG	1 cut(s)	145
AcyI	GRCGYC	2 cut(s)	1972, 2352
AfaI	GTAC	6 cut(s)	317, 687, 1080, 1581, 2054, 2102
AfiI	CCNNNNNNNGG	5 cut(s)	37, 363, 590, 1897, 2183
AflII	CTTAAG	2 cut(s)	548, 701
AflIII	ACRYGT	1 cut(s)	142
AjiI	CACGTC	1 cut(s)	2318
AjnI	CCWGG	2 cut(s)	294, 1623
AleI	CACNNNNGTG	3 cut(s)	143, 1475, 1734
Alw21I	GWGCWC	2 cut(s)	60, 1731
Alw26I	GTCTC	4 cut(s)	127, 213, 424, 2267
AlwI	GGATC	5 cut(s)	869, 2034, 2194, 2325, 2338
AlwNI	CAGNNNCTG	2 cut(s)	688, 2189
AoxI	GGCC	6 cut(s)	28, 48, 97, 260, 283, 427
ApeKI	GCWGC	3 cut(s)	833, 1121, 1143
ApoI	RAATTY	3 cut(s)	1227, 1433, 1845
Asp700I	GAANNNNTTC	1 cut(s)	1416
AspLEI	GCGC	2 cut(s)	105, 2354
AspS9I	GGNCC	4 cut(s)	743, 800, 1323, 1520
AsuHPI	GGTGA	9 cut(s)	20, 101, 215, 797, 1489, 1748, 1916, 2195, 2264
AsuII	TTCGAA	1 cut(s)	1420
AvaII	GGWCC	4 cut(s)	743, 800, 1323, 1520
AxyI	CCTNAGG	2 cut(s)	1926, 2022
BamHI	GGATCC	1 cut(s)	2330
BanI	GGYRCC	1 cut(s)	2351
BanII	GRGCYC	3 cut(s)	60, 357, 1464
BauI	CACGAG	2 cut(s)	380, 542
BbrPI	CACGTG	1 cut(s)	145
BbsI	GAAGAC	2 cut(s)	698, 2292
Bbv12I	GWGCWC	2 cut(s)	60, 1731
BbvI	GCAGC	3 cut(s)	845, 1130, 1133
BccI	CCATC	5 cut(s)	28, 258, 275, 775, 2356
BceAI	ACGGC	1 cut(s)	175
BciT130I	CCWGG	2 cut(s)	296, 1625
BclI	TGATCA	2 cut(s)	1177, 2134
BcoDI	GTCTC	4 cut(s)	127, 213, 424, 2267
BfaI	CTAG	5 cut(s)	233, 804, 980, 1106, 2279
BfmI	CTRYAG	1 cut(s)	1119
BfoI	RGCGCY	1 cut(s)	2355
BfrI	CTTAAG	2 cut(s)	548, 701
BfuAI	ACCTGC	1 cut(s)	673
BglII	AGATCT	3 cut(s)	1064, 1501, 1947
BisI	GCNGC	4 cut(s)	414, 834, 1122, 1144
BlsI	GCNGC	4 cut(s)	415, 835, 1123, 1145
Bme1390I	CCNGG	2 cut(s)	296, 1625
Bme18I	GGWCC	4 cut(s)	743, 800, 1323, 1520
BmgBI	CACGTC	1 cut(s)	2318
BmgT120I	GGNCC	4 cut(s)	743, 800, 1323, 1520
BmiI	GGNNCC	5 cut(s)	354, 744, 1522, 2332, 2353
BmrFI	CCNGG	2 cut(s)	296, 1625
BmsI	GCATC	2 cut(s)	366, 627
BpiI	GAAGAC	2 cut(s)	698, 2292
Bpu10I	CCTNAGC	1 cut(s)	2010
Bpu14I	TTCGAA	1 cut(s)	1420
BpuEI	CTTGAG	2 cut(s)	984, 1054
Bsa29I	ATCGAT	1 cut(s)	559
BsaAI	YACGTR	1 cut(s)	145
BsaHI	GRCGYC	2 cut(s)	1972, 2352
BsaJI	CCNNGG	6 cut(s)	51, 129, 199, 1264, 1732, 2355
Bsc4I	CCNNNNNNNGG	5 cut(s)	37, 363, 590, 1897, 2183
Bse118I	RCCGGY	1 cut(s)	99
Bse1I	ACTGG	4 cut(s)	834, 1479, 1547, 2226
Bse21I	CCTNAGG	2 cut(s)	1926, 2022
Bse3DI	GCAATG	5 cut(s)	339, 345, 695, 1447, 1794
BseBI	CCWGG	2 cut(s)	296, 1625
BseCI	ATCGAT	1 cut(s)	559
BseDI	CCNNGG	6 cut(s)	51, 129, 199, 1264, 1732, 2355
BseGI	GGATG	6 cut(s)	39, 250, 381, 1393, 1492, 2367
BseLI	CCNNNNNNNGG	5 cut(s)	37, 363, 590, 1897, 2183
BseMI	GCAATG	5 cut(s)	339, 345, 695, 1447, 1794
BseMII	CTCAG	6 cut(s)	435, 729, 1824, 1917, 2036, 2105
BseNI	ACTGG	4 cut(s)	834, 1479, 1547, 2226
BseRI	GAGGAG	3 cut(s)	68, 956, 2063
BseXI	GCAGC	3 cut(s)	845, 1130, 1133
BseYI	CCCAGC	1 cut(s)	1274
Bsh1285I	CGRYCG	2 cut(s)	175, 1016
BshFI	GGCC	6 cut(s)	30, 50, 99, 262, 285, 429
BshNI	GGYRCC	1 cut(s)	2351
BshVI	ATCGAT	1 cut(s)	559
BsiEI	CGRYCG	2 cut(s)	175, 1016
BsiHKAI	GWGCWC	2 cut(s)	60, 1731
BsiSI	CCGG	3 cut(s)	31, 100, 286
BslFI	GGGAC	2 cut(s)	1506, 1886
BslI	CCNNNNNNNGG	5 cut(s)	37, 363, 590, 1897, 2183
BsmAI	GTCTC	4 cut(s)	127, 213, 424, 2267
BsmFI	GGGAC	2 cut(s)	1506, 1886
BsmI	GAATGC	2 cut(s)	400, 2060
BsnI	GGCC	6 cut(s)	30, 50, 99, 262, 285, 429
Bsp119I	TTCGAA	1 cut(s)	1420
Bsp1286I	GDGCHC	4 cut(s)	60, 357, 1464, 1731
Bsp1407I	TGTACA	2 cut(s)	315, 1579
Bsp19I	CCATGG	1 cut(s)	199
BspACI	CCGC	8 cut(s)	171, 196, 350, 398, 413, 665, 1089, 1563
BspANI	GGCC	6 cut(s)	30, 50, 99, 262, 285, 429
BspCNI	CTCAG	6 cut(s)	434, 730, 1825, 1918, 2035, 2104
BspDI	ATCGAT	1 cut(s)	559
BspHI	TCATGA	1 cut(s)	1720
BspLI	GGNNCC	5 cut(s)	354, 744, 1522, 2332, 2353
BspMAI	CTGCAG	1 cut(s)	1123
BspMI	ACCTGC	1 cut(s)	673
BspPI	GGATC	5 cut(s)	869, 2034, 2194, 2325, 2338
BspQI	GCTCTTC	2 cut(s)	1022, 1719
BspT104I	TTCGAA	1 cut(s)	1420
BspT107I	GGYRCC	1 cut(s)	2351
BspTI	CTTAAG	2 cut(s)	548, 701
BsrDI	GCAATG	5 cut(s)	339, 345, 695, 1447, 1794
BsrFI	RCCGGY	1 cut(s)	99
BsrGI	TGTACA	2 cut(s)	315, 1579
BsrI	ACTGG	4 cut(s)	834, 1479, 1547, 2226
BssAI	RCCGGY	1 cut(s)	99
BssECI	CCNNGG	6 cut(s)	51, 129, 199, 1264, 1732, 2355
BssNI	GRCGYC	2 cut(s)	1972, 2352
BssSI	CACGAG	2 cut(s)	380, 542
BssT1I	CCWWGG	3 cut(s)	129, 199, 2355
Bst2BI	CACGAG	2 cut(s)	380, 542
Bst2UI	CCWGG	2 cut(s)	296, 1625
Bst4CI	ACNGT	8 cut(s)	769, 967, 1017, 1584, 1716, 1733, 1885, 2207
Bst6I	CTCTTC	3 cut(s)	1022, 1631, 1719
BstACI	GRCGYC	2 cut(s)	1972, 2352
BstAFI	CTTAAG	2 cut(s)	548, 701
BstAPI	GCANNNNNTGC	1 cut(s)	1163
BstAUI	TGTACA	2 cut(s)	315, 1579
BstBAI	YACGTR	1 cut(s)	145
BstBI	TTCGAA	1 cut(s)	1420
BstC8I	GCNNGC	9 cut(s)	60, 101, 365, 911, 1454, 1662, 1699, 2120, 2168
BstDEI	CTNAG	9 cut(s)	421, 738, 1833, 1896, 1926, 2010, 2022, 2091, 2269
BstDSI	CCRYGG	3 cut(s)	199, 1264, 1732
BstENI	CCTNNNNNAGG	2 cut(s)	1895, 2181
BstF5I	GGATG	6 cut(s)	39, 250, 381, 1393, 1492, 2367
BstH2I	RGCGCY	1 cut(s)	2355
BstHHI	GCGC	2 cut(s)	105, 2354
BstMAI	GTCTC	4 cut(s)	127, 213, 424, 2267
BstMCI	CGRYCG	2 cut(s)	175, 1016
BstMWI	GCNNNNNNNGC	3 cut(s)	168, 839, 1163
BstNI	CCWGG	2 cut(s)	296, 1625
BstNSI	RCATGY	3 cut(s)	1400, 1701, 1961
BstSCI	CCNGG	2 cut(s)	294, 1623
BstSFI	CTRYAG	1 cut(s)	1119
BstV1I	GCAGC	3 cut(s)	845, 1130, 1133
BstV2I	GAAGAC	2 cut(s)	698, 2292
BstX2I	RGATCY	5 cut(s)	1064, 1501, 1947, 2186, 2330
BstXI	CCANNNNNNTGG	1 cut(s)	2362
BstYI	RGATCY	5 cut(s)	1064, 1501, 1947, 2186, 2330
Bsu15I	ATCGAT	1 cut(s)	559
Bsu36I	CCTNAGG	2 cut(s)	1926, 2022
BsuRI	GGCC	6 cut(s)	30, 50, 99, 262, 285, 429
BsuTUI	ATCGAT	1 cut(s)	559
BtgI	CCRYGG	3 cut(s)	199, 1264, 1732
BtrI	CACGTC	1 cut(s)	2318
BtsCI	GGATG	6 cut(s)	39, 250, 381, 1393, 1492, 2367
BtsI	GCAGTG	1 cut(s)	1464
BtsIMutI	CAGTG	4 cut(s)	1187, 1464, 1472, 1589
BveI	ACCTGC	1 cut(s)	673
Cac8I	GCNNGC	9 cut(s)	60, 101, 365, 911, 1454, 1662, 1699, 2120, 2168
CaiI	CAGNNNCTG	2 cut(s)	688, 2189
CciI	TCATGA	1 cut(s)	1720
CfoI	GCGC	2 cut(s)	105, 2354
Cfr10I	RCCGGY	1 cut(s)	99
Cfr13I	GGNCC	4 cut(s)	743, 800, 1323, 1520
ClaI	ATCGAT	1 cut(s)	559
CseI	GACGC	2 cut(s)	6, 1976
Csp6I	GTAC	6 cut(s)	316, 686, 1079, 1580, 2053, 2101
CviQI	GTAC	6 cut(s)	316, 686, 1079, 1580, 2053, 2101
DdeI	CTNAG	9 cut(s)	421, 738, 1833, 1896, 1926, 2010, 2022, 2091, 2269
DinI	GGCGCC	1 cut(s)	2353
DraI	TTTAAA	1 cut(s)	660
EaeI	YGGCCR	3 cut(s)	28, 48, 283
Eam1104I	CTCTTC	3 cut(s)	1022, 1631, 1719
EarI	CTCTTC	3 cut(s)	1022, 1631, 1719
EciI	GGCGGA	2 cut(s)	185, 1578
Ecl136II	GAGCTC	1 cut(s)	58
Eco130I	CCWWGG	3 cut(s)	129, 199, 2355
Eco24I	GRGCYC	3 cut(s)	60, 357, 1464
Eco47I	GGWCC	4 cut(s)	743, 800, 1323, 1520
Eco53kI	GAGCTC	1 cut(s)	58
Eco57I	CTGAAG	4 cut(s)	710, 864, 1611, 2291
Eco72I	CACGTG	1 cut(s)	145
Eco81I	CCTNAGG	2 cut(s)	1926, 2022
EcoICRI	GAGCTC	1 cut(s)	58
EcoNI	CCTNNNNNAGG	2 cut(s)	1895, 2181
EcoO109I	RGGNCCY	1 cut(s)	743
EcoRII	CCWGG	2 cut(s)	294, 1623
EcoT14I	CCWWGG	3 cut(s)	129, 199, 2355
EcoT22I	ATGCAT	2 cut(s)	865, 2062
EcoT38I	GRGCYC	3 cut(s)	60, 357, 1464
EgeI	GGCGCC	1 cut(s)	2353
EheI	GGCGCC	1 cut(s)	2353
ErhI	CCWWGG	3 cut(s)	129, 199, 2355
FalI	AAGNNNNNCTT	4 cut(s)	695, 727, 718, 750
FaqI	GGGAC	2 cut(s)	1506, 1886
FauI	CCCGC	1 cut(s)	405
FbaI	TGATCA	2 cut(s)	1177, 2134
FblI	GTMKAC	2 cut(s)	2017, 2081
Fnu4HI	GCNGC	4 cut(s)	414, 834, 1122, 1144
FokI	GGATG	6 cut(s)	46, 237, 388, 1400, 1499, 2374
FriOI	GRGCYC	3 cut(s)	60, 357, 1464
Fsp4HI	GCNGC	4 cut(s)	414, 834, 1122, 1144
FspBI	CTAG	5 cut(s)	233, 804, 980, 1106, 2279
GlaI	GCGC	2 cut(s)	104, 2353
GluI	GCNGC	4 cut(s)	414, 834, 1122, 1144
GsaI	CCCAGC	1 cut(s)	1278
HaeII	RGCGCY	1 cut(s)	2355
HaeIII	GGCC	6 cut(s)	30, 50, 99, 262, 285, 429
HapII	CCGG	3 cut(s)	31, 100, 286
HgaI	GACGC	2 cut(s)	6, 1976
HhaI	GCGC	2 cut(s)	105, 2354
Hin1I	GRCGYC	2 cut(s)	1972, 2352
Hin6I	GCGC	2 cut(s)	103, 2352
HinP1I	GCGC	2 cut(s)	103, 2352
HincII	GTYRAC	3 cut(s)	970, 1209, 2082
HindII	GTYRAC	3 cut(s)	970, 1209, 2082
HindIII	AAGCTT	4 cut(s)	324, 1454, 1854, 2164
HinfI	GANTC	8 cut(s)	556, 572, 722, 917, 1260, 1331, 1678, 2180
HpaI	GTTAAC	1 cut(s)	970
HpaII	CCGG	3 cut(s)	31, 100, 286
HphI	GGTGA	9 cut(s)	20, 101, 215, 797, 1489, 1748, 1916, 2195, 2264
Hpy166II	GTNNAC	7 cut(s)	627, 970, 1209, 1879, 2018, 2082, 2368
Hpy8I	GTNNAC	7 cut(s)	627, 970, 1209, 1879, 2018, 2082, 2368
Hpy99I	CGWCG	2 cut(s)	22, 2322
HpyAV	CCTTC	4 cut(s)	80, 200, 888, 2315
HpyCH4III	ACNGT	8 cut(s)	769, 967, 1017, 1584, 1716, 1733, 1885, 2207
HpyCH4IV	ACGT	6 cut(s)	144, 904, 1875, 1972, 1996, 2317
HpyF10VI	GCNNNNNNNGC	3 cut(s)	168, 839, 1163
HpyF3I	CTNAG	9 cut(s)	421, 738, 1833, 1896, 1926, 2010, 2022, 2091, 2269
HpySE526I	ACGT	6 cut(s)	144, 904, 1875, 1972, 1996, 2317
Hsp92I	GRCGYC	2 cut(s)	1972, 2352
HspAI	GCGC	2 cut(s)	103, 2352
KasI	GGCGCC	1 cut(s)	2351
KroI	GCCGGC	1 cut(s)	99
KroNI	GCCGGC	1 cut(s)	101
Ksp22I	TGATCA	2 cut(s)	1177, 2134
KspAI	GTTAAC	1 cut(s)	970
LguI	GCTCTTC	2 cut(s)	1022, 1719
LmnI	GCTCC	4 cut(s)	55, 352, 486, 794
Lsp1109I	GCAGC	3 cut(s)	845, 1130, 1133
LweI	GCATC	2 cut(s)	366, 627
MaeI	CTAG	5 cut(s)	233, 804, 980, 1106, 2279
MaeII	ACGT	6 cut(s)	144, 904, 1875, 1972, 1996, 2317
MaeIII	GTNAC	1 cut(s)	1285
MflI	RGATCY	5 cut(s)	1064, 1501, 1947, 2186, 2330
MhlI	GDGCHC	4 cut(s)	60, 357, 1464, 1731
Mly113I	GGCGCC	1 cut(s)	2352
MlyI	GAGTC	1 cut(s)	581
MmeI	TCCRAC	2 cut(s)	918, 1527
Mph1103I	ATGCAT	2 cut(s)	865, 2062
MroNI	GCCGGC	1 cut(s)	99
MroXI	GAANNNNTTC	1 cut(s)	1416
MseI	TTAA	9 cut(s)	549, 659, 702, 897, 969, 1197, 1557, 2151, 2389
MslI	CAYNNNNRTG	5 cut(s)	143, 753, 779, 1475, 1734
MspA1I	CMGCKG	2 cut(s)	171, 836
MspCI	CTTAAG	2 cut(s)	548, 701
MspI	CCGG	3 cut(s)	31, 100, 286
MspR9I	CCNGG	2 cut(s)	296, 1625
Mva1269I	GAATGC	2 cut(s)	400, 2060
MvaI	CCWGG	2 cut(s)	296, 1625
MwoI	GCNNNNNNNGC	3 cut(s)	168, 839, 1163
NaeI	GCCGGC	1 cut(s)	101
NarI	GGCGCC	1 cut(s)	2352
NcoI	CCATGG	1 cut(s)	199
NgoMIV	GCCGGC	1 cut(s)	99
NlaIV	GGNNCC	5 cut(s)	354, 744, 1522, 2332, 2353
NmeAIII	GCCGAG	2 cut(s)	76, 287
NmuCI	GTSAC	1 cut(s)	1285
NsiI	ATGCAT	2 cut(s)	865, 2062
NspI	RCATGY	3 cut(s)	1400, 1701, 1961
NspV	TTCGAA	1 cut(s)	1420
OliI	CACNNNNGTG	3 cut(s)	143, 1475, 1734
PaeI	GCATGC	1 cut(s)	1701
PagI	TCATGA	1 cut(s)	1720
PciSI	GCTCTTC	2 cut(s)	1022, 1719
PcsI	WCGNNNNNNNCGW	2 cut(s)	150, 1881
PctI	GAATGC	2 cut(s)	400, 2060
PdiI	GCCGGC	1 cut(s)	101
PdmI	GAANNNNTTC	1 cut(s)	1416
PfeI	GAWTC	7 cut(s)	556, 722, 917, 1260, 1331, 1678, 2180
PflFI	GACNNNGTC	1 cut(s)	191
PfoI	TCCNGGA	2 cut(s)	294, 1623
PkrI	GCNGC	4 cut(s)	415, 835, 1123, 1145
PleI	GAGTC	1 cut(s)	580
PluTI	GGCGCC	1 cut(s)	2355
PmaCI	CACGTG	1 cut(s)	145
PmlI	CACGTG	1 cut(s)	145
PpsI	GAGTC	1 cut(s)	580
Ppu21I	YACGTR	1 cut(s)	145
PpuMI	RGGWCCY	1 cut(s)	743
PsiI	TTATAA	1 cut(s)	672
Psp124BI	GAGCTC	1 cut(s)	60
Psp1406I	AACGTT	1 cut(s)	904
Psp5II	RGGWCCY	1 cut(s)	743
Psp6I	CCWGG	2 cut(s)	294, 1623
PspCI	CACGTG	1 cut(s)	145
PspFI	CCCAGC	1 cut(s)	1274
PspGI	CCWGG	2 cut(s)	294, 1623
PspN4I	GGNNCC	5 cut(s)	354, 744, 1522, 2332, 2353
PspPI	GGNCC	4 cut(s)	743, 800, 1323, 1520
PspPPI	RGGWCCY	1 cut(s)	743
PstI	CTGCAG	1 cut(s)	1123
PstNI	CAGNNNCTG	2 cut(s)	688, 2189
PsuI	RGATCY	5 cut(s)	1064, 1501, 1947, 2186, 2330
PsyI	GACNNNGTC	1 cut(s)	191
PvuII	CAGCTG	1 cut(s)	836
RsaI	GTAC	6 cut(s)	317, 687, 1080, 1581, 2054, 2102
RsaNI	GTAC	6 cut(s)	316, 686, 1079, 1580, 2053, 2101
RseI	CAYNNNNRTG	5 cut(s)	143, 753, 779, 1475, 1734
SacI	GAGCTC	1 cut(s)	60
SalI	GTCGAC	1 cut(s)	2080
SapI	GCTCTTC	2 cut(s)	1022, 1719
SaqAI	TTAA	9 cut(s)	549, 659, 702, 897, 969, 1197, 1557, 2151, 2389
SatI	GCNGC	4 cut(s)	414, 834, 1122, 1144
Sau96I	GGNCC	4 cut(s)	743, 800, 1323, 1520
SchI	GAGTC	1 cut(s)	581
ScrFI	CCNGG	2 cut(s)	296, 1625
SduI	GDGCHC	4 cut(s)	60, 357, 1464, 1731
SfaNI	GCATC	2 cut(s)	366, 627
SfcI	CTRYAG	1 cut(s)	1119
SfoI	GGCGCC	1 cut(s)	2353
SfuI	TTCGAA	1 cut(s)	1420
SinI	GGWCC	4 cut(s)	743, 800, 1323, 1520
SmiI	ATTTAAAT	1 cut(s)	660
SmiMI	CAYNNNNRTG	5 cut(s)	143, 753, 779, 1475, 1734
SmlI	CTYRAG	4 cut(s)	548, 701, 999, 1033
SmoI	CTYRAG	4 cut(s)	548, 701, 999, 1033
SphI	GCATGC	1 cut(s)	1701
SsiI	CCGC	8 cut(s)	171, 196, 350, 398, 413, 665, 1089, 1563
SspDI	GGCGCC	1 cut(s)	2351
SspI	AATATT	1 cut(s)	1631
SspMI	CTAG	5 cut(s)	233, 804, 980, 1106, 2279
SstI	GAGCTC	1 cut(s)	60
StyD4I	CCNGG	2 cut(s)	294, 1623
StyI	CCWWGG	3 cut(s)	129, 199, 2355
SwaI	ATTTAAAT	1 cut(s)	660
TaaI	ACNGT	8 cut(s)	769, 967, 1017, 1584, 1716, 1733, 1885, 2207
TaiI	ACGT	6 cut(s)	147, 907, 1878, 1975, 1999, 2320
TaqI	TCGA	6 cut(s)	20, 559, 570, 1129, 1420, 2081
TatI	WGTACW	5 cut(s)	315, 1078, 1579, 2052, 2100
TauI	GCSGC	1 cut(s)	416
TfiI	GAWTC	7 cut(s)	556, 722, 917, 1260, 1331, 1678, 2180
Tru1I	TTAA	9 cut(s)	549, 659, 702, 897, 969, 1197, 1557, 2151, 2389
Tru9I	TTAA	9 cut(s)	549, 659, 702, 897, 969, 1197, 1557, 2151, 2389
TscAI	CASTG	4 cut(s)	1187, 1471, 1479, 1589
TseFI	GTSAC	1 cut(s)	1285
TseI	GCWGC	3 cut(s)	833, 1121, 1143
Tsp45I	GTSAC	1 cut(s)	1285
TspDTI	ATGAA	8 cut(s)	493, 771, 834, 1548, 1691, 1709, 1737, 1805
TspGWI	ACGGA	2 cut(s)	1162, 1253
TspRI	CASTG	4 cut(s)	1187, 1471, 1479, 1589
Tth111I	GACNNNGTC	1 cut(s)	191
Vha464I	CTTAAG	2 cut(s)	548, 701
VpaK11BI	GGWCC	4 cut(s)	743, 800, 1323, 1520
XagI	CCTNNNNNAGG	2 cut(s)	1895, 2181
XapI	RAATTY	3 cut(s)	1227, 1433, 1845
XbaI	TCTAGA	1 cut(s)	979
XceI	RCATGY	3 cut(s)	1400, 1701, 1961
XmiI	GTMKAC	2 cut(s)	2017, 2081
XmnI	GAANNNNTTC	1 cut(s)	1416
XspI	CTAG	5 cut(s)	233, 804, 980, 1106, 2279
ZraI	GACGTC	1 cut(s)	1973
Zsp2I	ATGCAT	2 cut(s)	865, 2062

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.