MD07G1029700.v1.1

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0002404
Physical Location & Seq
Reverse (-)
2479289 .. 2485298
6010 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1029700.v1.1.491

Sequence Viewer

Length: 1899 bp
ATGGGGTCCTATAGGGTTTCCAATTATTCATTCGTTTTGTTCATATTGTTTCTGGGTTCTTTTGCTTCTTCTTTTTCATCATCCTTGCACCCTTTGGTTTTCAATTCCAAAGTTGAACACCTTAATCACACAGCAATATCTGAGTTTAGGGTTCTTAACAGAAGATTTCTAGGCGACTGTCCGAATCCGAGCCCTTATCTGAAAATTAGTACTAATTCAAGTGCTTCTGGCCTTGGAGATGAAGAATATGTGAGTGTCAATGTTAGTGGCGTCCTGCGTCCTTCCAAAGACGATTGGGTCGCCATGATATCACCTTCTCATTCTGATATGTCAAGTTGCCTTGCCAATGGAATTCTCTATGCACAAACTGGAGATTTTGAAAAACTACCTCTTCTCTGCCATTATCCTGTCAAGGCAGCATATATGAGCAATGATCCTGACTATCTCAGCTGCAAGAAGAAGGAATGCAAAAAGTATCAGAATGGTCGATGCGTGGTGTCCACTTGTGGTGGTTCATTATCATTTCATGTTATCAACATCAGAACCGACATTGAATTTGTGTTGTTTTCCGGTGGATTTGTGGCACCTTGTATTCTCAAGAAGTCAAACCCTCTGAAATTTACCAATCCGAACAAGCCATTATATGGACATATCTCAAGCATCGATTCAACTGGAACATCGATGAGAGTAACATGGGTTAGTGGGCACAAGGAACCTCAGCAAGTGCAATATGGCAACGGGAAAAAGCAGACATCAGGAGTTACTACATTTTCACAACACGATATGAAAAGTTCAGCTCTACCAAGTCCAGCCAAGGACTTCCGCTGGCATGATCCAGGTTTCATTCATACAGCAGTAATGAGAGGACTTAAGCCTTCATCTAGTTTCTCTTATCGATATGGAAGCGATTCAGTTGGTTGGAGTGATCAAATCCAATTCCGGACTCCACCAGCAGGAGGGTCTGATGAACTCAAGTTTCTTGCATTTGGCGACATGGGAAAGGCTCCTCGGGATTCTTCGACGATGCATTACATTCAGCCAGGATCTCTTTCAGTGATTCAAGCCGTAGCCAATGAAATAAATTCCGGGAATGTAGACTCTATCTTCCACATTGGAGACATTAGCTATGCCACAGGATTTTTAGTTGAATGGGATCTTTTCCTTCAACAAATCAGTCCGGTTGCTTCTCGAGTTTCTTACATGACAGCAATAGGAAACCATGAGAGGGACTACATAGACACTGGATCGGTTTATATTTTACCAGATTCAGGTGGAGAATCTGGAGTTCCTTATGAGACTTACTTTCCAATGCCAACCTCAGCAAAGGATAAACCATGGTATTCCATAGAACAAGCAAGTGTTCACATCACGGTGATTTCAACAGAGCATGACTGGTCCACAAATTCTGAGCAGTATCAATGGATGAGAAAGGACATGGCTTCAGTTGATCGATCTAAAACTCCTTGGTTGATTGTTATGGGGCATAGACCCATGTATTCCTCCTCACCTGGATTCCTCAATGTTGATAAAATATTTGTCAACGAAGTGGAACCATTACTGCTCGAGAGCAAAGTTGACTTAGTCCTATTTGGTCATGTCCATAACTACGAGCGAACTTGTGCGGTTTATAAGAGTGAATGCAAAGGATTGCCTCAAAAAAATGAAAATGGGATCGACACATATGATCATAGCAACTACAGTGCCCCAGTGCACGCTGTAATTGGTATGGCTGGCTTTACCTTGGACGGGTTCTCAAGTGGTGTTAATAATCCTTGGAGTTTATCCAAGATTTCTGAATTTGGTTATCTAAGAGGACATGCGACAAAGAAAGAGATGAAATTAGAGCTTGTGAATGCAGATACAAGAAAGGTTGAAGACGGATTCCTCATTACAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003993 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0044237
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

633

Amino Acids

70.11

Weight (kDa)

6.62

Isoelectric Point (pI)

42.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
fn3_PAP PF17808 75 - 209 6.1e-34 Fn3-like domain from Purple Acid Phosphatase
Pur_ac_phosph_N PF16656 216 - 315 2.7e-16 Purple acid Phosphatase, N-terminal domain
Metallophos PF00149 324 - 536 3e-18 Calcineurin-like phosphoesterase
Metallophos_C PF14008 568 - 626 2.1e-07 Iron/zinc purple acid phosphatase-like protein C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1629
AasI GACNNNNNNGTC 1 cut(s) 296
AccB1I GGYRCC 1 cut(s) 583
AccB7I CCANNNNNTGG 1 cut(s) 644
AccI GTMKAC 1 cut(s) 1095
AccIII TCCGGA 1 cut(s) 939
AciI CCGC 2 cut(s) 823, 1622
AclWI GGATC 6 cut(s) 428, 827, 1051, 1161, 1252, 1679
AcsI RAATTY 6 cut(s) 351, 554, 617, 1081, 1402, 1796
AcuI CTGAAG 1 cut(s) 1425
AcyI GRCGYC 1 cut(s) 270
AfaI GTAC 1 cut(s) 211
AfiI CCNNNNNNNGG 6 cut(s) 644, 953, 956, 1225, 1268, 1746
AflII CTTAAG 1 cut(s) 869
AjnI CCWGG 3 cut(s) 835, 1039, 1507
AjuI GAANNNNNNNTTGG 2 cut(s) 14, 46
AluBI AGCT 4 cut(s) 450, 797, 1125, 1846
AluI AGCT 4 cut(s) 450, 797, 1125, 1846
Alw21I GWGCWC 1 cut(s) 1713
Alw26I GTCTC 2 cut(s) 1110, 1289
Alw44I GTGCAC 1 cut(s) 1709
AlwI GGATC 6 cut(s) 428, 827, 1051, 1161, 1252, 1679
Ama87I CYCGRG 3 cut(s) 1008, 1188, 1562
Aor13HI TCCGGA 1 cut(s) 939
AoxI GGCC 1 cut(s) 229
ApaLI GTGCAC 1 cut(s) 1709
ApeKI GCWGC 2 cut(s) 416, 450
ApoI RAATTY 6 cut(s) 351, 554, 617, 1081, 1402, 1796
ArsI GACNNNNNNTTYG 2 cut(s) 539, 571
Asp700I GAANNNNTTC 1 cut(s) 907
AspS9I GGNCC 2 cut(s) 6, 1395
AsuC2I CCSGG 1 cut(s) 1087
AsuHPI GGTGA 3 cut(s) 303, 1384, 1497
AvaI CYCGRG 3 cut(s) 1008, 1188, 1562
AvaII GGWCC 2 cut(s) 6, 1395
BaeGI GKGCMC 3 cut(s) 708, 1705, 1713
BanI GGYRCC 1 cut(s) 583
BanII GRGCYC 1 cut(s) 194
BbsI GAAGAC 1 cut(s) 1881
Bbv12I GWGCWC 1 cut(s) 1713
BbvCI CCTCAGC 2 cut(s) 717, 1318
BbvI GCAGC 2 cut(s) 428, 437
BceAI ACGGC 1 cut(s) 1049
BciT130I CCWGG 3 cut(s) 837, 1041, 1509
BclI TGATCA 2 cut(s) 925, 1684
BcnI CCSGG 1 cut(s) 1087
BcoDI GTCTC 2 cut(s) 1110, 1289
BfaI CTAG 2 cut(s) 170, 882
BfmI CTRYAG 2 cut(s) 10, 1696
BfrI CTTAAG 1 cut(s) 869
BisI GCNGC 2 cut(s) 417, 451
BlsI GCNGC 2 cut(s) 418, 452
BmcAI AGTACT 1 cut(s) 211
Bme1390I CCNGG 4 cut(s) 837, 1041, 1087, 1509
Bme18I GGWCC 2 cut(s) 6, 1395
BmeT110I CYCGRG 3 cut(s) 1008, 1188, 1562
BmgT120I GGNCC 2 cut(s) 6, 1395
BmiI GGNNCC 5 cut(s) 7, 585, 714, 1005, 1551
BmrFI CCNGG 4 cut(s) 837, 1041, 1087, 1509
BmrI ACTGGG 1 cut(s) 1700
BmsI GCATC 3 cut(s) 479, 669, 1014
BmuI ACTGGG 1 cut(s) 1700
BpiI GAAGAC 1 cut(s) 1881
BpmI CTGGAG 2 cut(s) 390, 1302
Bpu10I CCTNAGC 2 cut(s) 717, 1318
BpuEI CTTGAG 4 cut(s) 581, 640, 956, 1738
BpuMI CCSGG 1 cut(s) 1087
Bsa29I ATCGAT 4 cut(s) 663, 680, 895, 1450
BsaHI GRCGYC 1 cut(s) 270
BsaJI CCNNGG 7 cut(s) 232, 813, 1007, 1334, 1463, 1740, 1772
BsaWI WCCGGW 3 cut(s) 569, 939, 1177
Bsc4I CCNNNNNNNGG 6 cut(s) 644, 953, 956, 1225, 1268, 1746
Bse1I ACTGG 5 cut(s) 373, 676, 1246, 1397, 1706
Bse3DI GCAATG 1 cut(s) 436
BseAI TCCGGA 1 cut(s) 939
BseBI CCWGG 3 cut(s) 837, 1041, 1509
BseCI ATCGAT 4 cut(s) 663, 680, 895, 1450
BseDI CCNNGG 7 cut(s) 232, 813, 1007, 1334, 1463, 1740, 1772
BseGI GGATG 2 cut(s) 80, 1428
BseLI CCNNNNNNNGG 6 cut(s) 644, 953, 956, 1225, 1268, 1746
BseMI GCAATG 1 cut(s) 436
BseMII CTCAG 5 cut(s) 132, 460, 731, 1332, 1398
BseNI ACTGG 5 cut(s) 373, 676, 1246, 1397, 1706
BseRI GAGGAG 2 cut(s) 996, 1492
BseSI GKGCMC 3 cut(s) 708, 1705, 1713
BseXI GCAGC 2 cut(s) 428, 437
BshFI GGCC 1 cut(s) 231
BshNI GGYRCC 1 cut(s) 583
BshVI ATCGAT 4 cut(s) 663, 680, 895, 1450
BsiHKAI GWGCWC 1 cut(s) 1713
BsiHKCI CYCGRG 3 cut(s) 1008, 1188, 1562
BsiSI CCGG 4 cut(s) 570, 940, 1086, 1178
BslFI GGGAC 1 cut(s) 1241
BslI CCNNNNNNNGG 6 cut(s) 644, 953, 956, 1225, 1268, 1746
BsmAI GTCTC 2 cut(s) 1110, 1289
BsmFI GGGAC 1 cut(s) 1241
BsmI GAATGC 3 cut(s) 470, 1643, 1858
BsnI GGCC 1 cut(s) 231
BsoBI CYCGRG 3 cut(s) 1008, 1188, 1562
Bsp1286I GDGCHC 4 cut(s) 194, 708, 1705, 1713
Bsp13I TCCGGA 1 cut(s) 939
Bsp19I CCATGG 1 cut(s) 1334
BspACI CCGC 2 cut(s) 823, 1622
BspANI GGCC 1 cut(s) 231
BspCNI CTCAG 5 cut(s) 133, 459, 730, 1331, 1399
BspDI ATCGAT 4 cut(s) 663, 680, 895, 1450
BspEI TCCGGA 1 cut(s) 939
BspLI GGNNCC 5 cut(s) 7, 585, 714, 1005, 1551
BspPI GGATC 6 cut(s) 428, 827, 1051, 1161, 1252, 1679
BspT107I GGYRCC 1 cut(s) 583
BspTI CTTAAG 1 cut(s) 869
BsrDI GCAATG 1 cut(s) 436
BsrI ACTGG 5 cut(s) 373, 676, 1246, 1397, 1706
BssECI CCNNGG 7 cut(s) 232, 813, 1007, 1334, 1463, 1740, 1772
BssNI GRCGYC 1 cut(s) 270
BssT1I CCWWGG 6 cut(s) 232, 813, 1334, 1463, 1740, 1772
Bst2UI CCWGG 3 cut(s) 837, 1041, 1509
Bst4CI ACNGT 3 cut(s) 179, 1372, 1700
Bst6I CTCTTC 1 cut(s) 396
BstACI GRCGYC 1 cut(s) 270
BstAFI CTTAAG 1 cut(s) 869
BstC8I GCNNGC 3 cut(s) 827, 1713, 1732
BstDEI CTNAG 7 cut(s) 141, 446, 717, 1318, 1407, 1579, 1808
BstDSI CCRYGG 1 cut(s) 1334
BstF5I GGATG 2 cut(s) 80, 1428
BstMAI GTCTC 2 cut(s) 1110, 1289
BstNI CCWGG 3 cut(s) 837, 1041, 1509
BstNSI RCATGY 1 cut(s) 1820
BstSCI CCNGG 4 cut(s) 835, 1039, 1085, 1507
BstSFI CTRYAG 2 cut(s) 10, 1696
BstSLI GKGCMC 3 cut(s) 708, 1705, 1713
BstV1I GCAGC 2 cut(s) 428, 437
BstV2I GAAGAC 1 cut(s) 1881
BstX2I RGATCY 2 cut(s) 1043, 1153
BstYI RGATCY 2 cut(s) 1043, 1153
Bsu15I ATCGAT 4 cut(s) 663, 680, 895, 1450
BsuRI GGCC 1 cut(s) 231
BsuTUI ATCGAT 4 cut(s) 663, 680, 895, 1450
BtgI CCRYGG 1 cut(s) 1334
BtsCI GGATG 2 cut(s) 80, 1428
BtsIMutI CAGTG 4 cut(s) 1059, 1239, 1705, 1713
Cac8I GCNNGC 3 cut(s) 827, 1713, 1732
Cfr13I GGNCC 2 cut(s) 6, 1395
ClaI ATCGAT 4 cut(s) 663, 680, 895, 1450
CseI GACGC 2 cut(s) 259, 266
Csp6I GTAC 1 cut(s) 210
CspCI CAANNNNNGTGG 2 cut(s) 247, 282
CviQI GTAC 1 cut(s) 210
DdeI CTNAG 7 cut(s) 141, 446, 717, 1318, 1407, 1579, 1808
DrdI GACNNNNNNGTC 1 cut(s) 296
DseDI GACNNNNNNGTC 1 cut(s) 296
Eam1104I CTCTTC 1 cut(s) 396
EarI CTCTTC 1 cut(s) 396
Eco130I CCWWGG 6 cut(s) 232, 813, 1334, 1463, 1740, 1772
Eco24I GRGCYC 1 cut(s) 194
Eco32I GATATC 1 cut(s) 309
Eco47I GGWCC 2 cut(s) 6, 1395
Eco57I CTGAAG 1 cut(s) 1425
Eco88I CYCGRG 3 cut(s) 1008, 1188, 1562
EcoO109I RGGNCCY 1 cut(s) 6
EcoRI GAATTC 1 cut(s) 351
EcoRII CCWGG 3 cut(s) 835, 1039, 1507
EcoRV GATATC 1 cut(s) 309
EcoT14I CCWWGG 6 cut(s) 232, 813, 1334, 1463, 1740, 1772
EcoT22I ATGCAT 1 cut(s) 1029
EcoT38I GRGCYC 1 cut(s) 194
ErhI CCWWGG 6 cut(s) 232, 813, 1334, 1463, 1740, 1772
FaqI GGGAC 1 cut(s) 1241
FauNDI CATATG 1 cut(s) 1681
FbaI TGATCA 2 cut(s) 925, 1684
FblI GTMKAC 1 cut(s) 1095
Fnu4HI GCNGC 2 cut(s) 417, 451
FokI GGATG 2 cut(s) 67, 1435
FriOI GRGCYC 1 cut(s) 194
Fsp4HI GCNGC 2 cut(s) 417, 451
FspBI CTAG 2 cut(s) 170, 882
GluI GCNGC 2 cut(s) 417, 451
GsuI CTGGAG 2 cut(s) 390, 1302
HaeIII GGCC 1 cut(s) 231
HapII CCGG 4 cut(s) 570, 940, 1086, 1178
HgaI GACGC 2 cut(s) 259, 266
Hin1I GRCGYC 1 cut(s) 270
HincII GTYRAC 2 cut(s) 1540, 1576
HindII GTYRAC 2 cut(s) 1540, 1576
HpaII CCGG 4 cut(s) 570, 940, 1086, 1178
HphI GGTGA 3 cut(s) 303, 1384, 1497
Hpy166II GTNNAC 7 cut(s) 501, 1096, 1363, 1398, 1540, 1576, 1711
Hpy188III TCNNGA 8 cut(s) 437, 598, 756, 940, 1010, 1188, 1281, 1564
Hpy8I GTNNAC 7 cut(s) 501, 1096, 1363, 1398, 1540, 1576, 1711
Hpy99I CGWCG 1 cut(s) 1024
HpyAV CCTTC 5 cut(s) 291, 324, 454, 885, 1172
HpyCH4III ACNGT 3 cut(s) 179, 1372, 1700
HpyF3I CTNAG 7 cut(s) 141, 446, 717, 1318, 1407, 1579, 1808
Hsp92I GRCGYC 1 cut(s) 270
Kpn2I TCCGGA 1 cut(s) 939
Ksp22I TGATCA 2 cut(s) 925, 1684
LmnI GCTCC 1 cut(s) 1009
Lsp1109I GCAGC 2 cut(s) 428, 437
LweI GCATC 3 cut(s) 479, 669, 1014
MaeI CTAG 2 cut(s) 170, 882
MaeIII GTNAC 2 cut(s) 688, 760
MboII GAAGA 8 cut(s) 60, 174, 254, 383, 469, 1008, 1096, 1886
MflI RGATCY 2 cut(s) 1043, 1153
MhlI GDGCHC 4 cut(s) 194, 708, 1705, 1713
MlyI GAGTC 2 cut(s) 937, 1091
MmeI TCCRAC 1 cut(s) 899
Mph1103I ATGCAT 1 cut(s) 1029
MroI TCCGGA 1 cut(s) 939
MroXI GAANNNNTTC 1 cut(s) 907
MseI TTAA 4 cut(s) 123, 156, 870, 1764
MslI CAYNNNNRTG 1 cut(s) 1370
MspA1I CMGCKG 2 cut(s) 450, 825
MspCI CTTAAG 1 cut(s) 869
MspI CCGG 4 cut(s) 570, 940, 1086, 1178
MspR9I CCNGG 4 cut(s) 837, 1041, 1087, 1509
Mva1269I GAATGC 3 cut(s) 470, 1643, 1858
MvaI CCWGG 3 cut(s) 837, 1041, 1509
NciI CCSGG 1 cut(s) 1087
NcoI CCATGG 1 cut(s) 1334
NdeI CATATG 1 cut(s) 1681
NlaIV GGNNCC 5 cut(s) 7, 585, 714, 1005, 1551
NsiI ATGCAT 1 cut(s) 1029
NspI RCATGY 1 cut(s) 1820
PaeR7I CTCGAG 2 cut(s) 1188, 1562
PctI GAATGC 3 cut(s) 470, 1643, 1858
PdmI GAANNNNTTC 1 cut(s) 907
PfeI GAWTC 9 cut(s) 184, 665, 908, 1013, 1057, 1265, 1277, 1512, 1881
PflFI GACNNNGTC 1 cut(s) 1580
PflMI CCANNNNNTGG 1 cut(s) 644
PfoI TCCNGGA 1 cut(s) 1085
PkrI GCNGC 2 cut(s) 418, 452
PleI GAGTC 2 cut(s) 937, 1091
PpsI GAGTC 2 cut(s) 937, 1091
PpuMI RGGWCCY 1 cut(s) 6
PsiI TTATAA 1 cut(s) 1629
Psp5II RGGWCCY 1 cut(s) 6
Psp6I CCWGG 3 cut(s) 835, 1039, 1507
PspGI CCWGG 3 cut(s) 835, 1039, 1507
PspN4I GGNNCC 5 cut(s) 7, 585, 714, 1005, 1551
PspPI GGNCC 2 cut(s) 6, 1395
PspPPI RGGWCCY 1 cut(s) 6
PsuI RGATCY 2 cut(s) 1043, 1153
PsyI GACNNNGTC 1 cut(s) 1580
PvuII CAGCTG 1 cut(s) 450
RsaI GTAC 1 cut(s) 211
RsaNI GTAC 1 cut(s) 210
RseI CAYNNNNRTG 1 cut(s) 1370
SaqAI TTAA 4 cut(s) 123, 156, 870, 1764
SatI GCNGC 2 cut(s) 417, 451
Sau96I GGNCC 2 cut(s) 6, 1395
ScaI AGTACT 1 cut(s) 211
SchI GAGTC 2 cut(s) 937, 1091
ScrFI CCNGG 4 cut(s) 837, 1041, 1087, 1509
SduI GDGCHC 4 cut(s) 194, 708, 1705, 1713
SfaNI GCATC 3 cut(s) 479, 669, 1014
SfcI CTRYAG 2 cut(s) 10, 1696
Sfr274I CTCGAG 2 cut(s) 1188, 1562
SinI GGWCC 2 cut(s) 6, 1395
SlaI CTCGAG 2 cut(s) 1188, 1562
SmiMI CAYNNNNRTG 1 cut(s) 1370
SmlI CTYRAG 7 cut(s) 596, 655, 869, 971, 1188, 1562, 1753
SmoI CTYRAG 7 cut(s) 596, 655, 869, 971, 1188, 1562, 1753
SsiI CCGC 2 cut(s) 823, 1622
SspI AATATT 1 cut(s) 1533
SspMI CTAG 2 cut(s) 170, 882
StyD4I CCNGG 4 cut(s) 835, 1039, 1085, 1507
StyI CCWWGG 6 cut(s) 232, 813, 1334, 1463, 1740, 1772
TaaI ACNGT 3 cut(s) 179, 1372, 1700
TaqI TCGA 9 cut(s) 487, 663, 680, 895, 1019, 1189, 1450, 1563, 1674
TatI WGTACW 1 cut(s) 209
TfiI GAWTC 9 cut(s) 184, 665, 908, 1013, 1057, 1265, 1277, 1512, 1881
Tru1I TTAA 4 cut(s) 123, 156, 870, 1764
Tru9I TTAA 4 cut(s) 123, 156, 870, 1764
TscAI CASTG 4 cut(s) 1059, 1246, 1705, 1713
TseI GCWGC 2 cut(s) 416, 450
TspGWI ACGGA 1 cut(s) 1893
TspRI CASTG 4 cut(s) 1059, 1246, 1705, 1713
Tth111I GACNNNGTC 1 cut(s) 1580
Van91I CCANNNNNTGG 1 cut(s) 644
Vha464I CTTAAG 1 cut(s) 869
VneI GTGCAC 1 cut(s) 1709
VpaK11BI GGWCC 2 cut(s) 6, 1395
XapI RAATTY 6 cut(s) 351, 554, 617, 1081, 1402, 1796
XceI RCATGY 1 cut(s) 1820
XhoI CTCGAG 2 cut(s) 1188, 1562
XmiI GTMKAC 1 cut(s) 1095
XmnI GAANNNNTTC 1 cut(s) 907
XspI CTAG 2 cut(s) 170, 882
ZrmI AGTACT 1 cut(s) 211
Zsp2I ATGCAT 1 cut(s) 1029
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.