Rh7BG311500

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0002404
Physical Location & Seq
Reverse (-)
30131301 .. 30139380
8080 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG311500.1

Sequence Viewer

Length: 1500 bp
ATGAAGTCAAATTCAAGTTTTGCAATATCTATCTCCATTTCCTCCTTGTCTCTTTCAGCAAGTACAACAACACCGCCACCAACATTCTTGTTTGCTATAGCAAGCTGCTTGAGAAGCGAACCCTGCAGAAAAGCAACATACATGAGCACTGATCCTGACTACCTCAGTTGCAAGAAGAAGGAATGCAAAAGTTATCAGAATGGTACCAGTCAATGTGCTATCTCCACCTGCAGTGGTTCATTAACATTTCATGTTATTAACATCAGAACCGACATTGAATTCGTCTTGTTTTCTGGTGAATTTGAGGTCCCTTGCATTTTGAAGAAAGCAAACCCTCTGAAATTTGCCAATCCAAACAAGCCACTGTATGGACATCTCTCGAGCATAGACTCAACTGGAACATCGATGAAATTAACATGGGTCAGTGGAGATGAGAAACCTCAGCAAGTACAATATGGAGATGGAAAATCACAGAGTTCAGTTACCACATTTTCACAAGATGACATGCAGAGTTCACCTGCTATTCCAAGTCCAGCCAAAGACTTTGGGTGGCATGATCCAGGCTTCATTCATTCAGCAGTGATGACAGGACTTAAGCCTTCATCTAGCTTCTCTTATAGATATGGAAGTGATTCAGTTGGTTGGAGTGATCGAATCCAATTCCACACTCCCCCTGTGGGAGGATCTGATGAACTCAAATTTCTTGCATTTGGCGACATGGGAAAGGCTCCTCGTGATTCTTCTGTCGAGCATTATATTCAGCCAGGATCCCTCTCAGTGATTGAGGCCGTGACGGATGAAATAAATTCCGAGAATGTAGACTCTGTCTTCCACATTGGAGATATAAGCTATGCCACTGGGTTTTTAGTTGAATGGGATTTTTTCCTTCACCAAATCAACCCTATTGCTTCTCGAGTTTCATACATGACAGCTATCGGGAACCATGAGAGGGACTATATAGACTCTGGATCAATGTACATTCTTGCAGATTCAGGTGGGGAAGTTGGAGTTCCCTATGAGACTTACTTTCCAATGCCAACCCCAGCAAAGGATAAACCATGGTATTCCATAGAACAAGGAAGCGTCCACATCACGATGATTTCAACAGAGCATGACTGGACCAAAAATTCTGAGCAGTATCAATGGATGAAAAAGGACATGGCTTCAGTTGATCGATCTAAAACTCCTTGGTTGATATTTATGGGGCATAGACCAATGTATACATCTGCCCCTGGATTCCTGAGTGTTGATGAGAAATTCGTTGACGAAGTTGAACCATTACTTCTGGCAAGCAAAGTTGATCTGGCCCTGTTTGGTCATGTCCATAACTACGAGCGAACCTGCTCCATTTATAAGAGCCAATGCATGAAATTGCCTTGCAAAGATGAGGCTGGGATCGACACATATGATCACAGCAACTACAGTGCCCCAGTGCAAGCCATAATTGGAATGGCTGGCTTTTCCTTGGACAAGTTTCCCAGTGGTGCAAGTCCTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003993 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0044237
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

499

Amino Acids

55.04

Weight (kDa)

5.37

Isoelectric Point (pI)

43.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
fn3_PAP PF17808 39 - 117 5.8e-14 Fn3-like domain from Purple Acid Phosphatase
Pur_ac_phosph_N PF16656 123 - 223 1.7e-15 Purple acid Phosphatase, N-terminal domain
Metallophos PF00149 232 - 444 9.2e-18 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1353
AarI CACCTGC 2 cut(s) 236, 526
Acc36I ACCTGC 3 cut(s) 236, 526, 1349
Acc65I GGTACC 1 cut(s) 203
AccB1I GGYRCC 1 cut(s) 203
AccB7I CCANNNNNTGG 1 cut(s) 368
AccI GTMKAC 2 cut(s) 819, 1220
AciI CCGC 1 cut(s) 74
AclWI GGATC 7 cut(s) 146, 551, 691, 762, 775, 976, 1403
AcsI RAATTY 8 cut(s) 10, 278, 299, 341, 698, 805, 1126, 1256
AcuI CTGAAG 1 cut(s) 1149
AfaI GTAC 4 cut(s) 64, 205, 450, 977
AfiI CCNNNNNNNGG 5 cut(s) 368, 677, 680, 949, 1048
AflII CTTAAG 1 cut(s) 593
AgsI TTSAA 6 cut(s) 15, 278, 322, 872, 1104, 1274
AjnI CCWGG 3 cut(s) 559, 763, 1231
AluBI AGCT 4 cut(s) 105, 609, 849, 932
AluI AGCT 4 cut(s) 105, 609, 849, 932
Alw21I GWGCWC 1 cut(s) 149
Alw26I GTCTC 2 cut(s) 54, 1013
AlwI GGATC 7 cut(s) 146, 551, 691, 762, 775, 976, 1403
Ama87I CYCGRG 2 cut(s) 379, 912
AoxI GGCC 2 cut(s) 786, 1305
ApeKI GCWGC 1 cut(s) 105
ApoI RAATTY 8 cut(s) 10, 278, 299, 341, 698, 805, 1126, 1256
ArsI GACNNNNNNTTYG 2 cut(s) 263, 295
Asp700I GAANNNNTTC 1 cut(s) 631
Asp718I GGTACC 1 cut(s) 203
AspS9I GGNCC 3 cut(s) 307, 1119, 1306
AsuHPI GGTGA 3 cut(s) 308, 507, 881
AvaI CYCGRG 2 cut(s) 379, 912
AvaII GGWCC 2 cut(s) 307, 1119
BaeGI GKGCMC 1 cut(s) 1429
BamHI GGATCC 1 cut(s) 767
BanI GGYRCC 1 cut(s) 203
BauI CACGAG 1 cut(s) 732
BbsI GAAGAC 1 cut(s) 820
Bbv12I GWGCWC 1 cut(s) 149
BbvCI CCTCAGC 1 cut(s) 441
BbvI GCAGC 1 cut(s) 92
BccI CCATC 1 cut(s) 455
BceAI ACGGC 1 cut(s) 773
BciT130I CCWGG 3 cut(s) 561, 765, 1233
BclI TGATCA 1 cut(s) 1408
BcoDI GTCTC 2 cut(s) 54, 1013
BfaI CTAG 1 cut(s) 606
BfmI CTRYAG 4 cut(s) 96, 124, 229, 1420
BfrI CTTAAG 1 cut(s) 593
BfuAI ACCTGC 3 cut(s) 236, 526, 1349
BisI GCNGC 1 cut(s) 106
BlsI GCNGC 1 cut(s) 107
Bme1390I CCNGG 3 cut(s) 561, 765, 1233
Bme18I GGWCC 2 cut(s) 307, 1119
BmeT110I CYCGRG 2 cut(s) 379, 912
BmgT120I GGNCC 3 cut(s) 307, 1119, 1306
BmiI GGNNCC 5 cut(s) 205, 309, 729, 769, 941
BmrFI CCNGG 3 cut(s) 561, 765, 1233
BmrI ACTGGG 3 cut(s) 867, 1424, 1473
BmuI ACTGGG 3 cut(s) 867, 1424, 1473
BpiI GAAGAC 1 cut(s) 820
Bpu10I CCTNAGC 1 cut(s) 441
BpuEI CTTGAG 1 cut(s) 130
Bsa29I ATCGAT 2 cut(s) 404, 1174
BsaJI CCNNGG 4 cut(s) 1058, 1187, 1231, 1464
Bsc4I CCNNNNNNNGG 5 cut(s) 368, 677, 680, 949, 1048
Bse1I ACTGG 6 cut(s) 207, 400, 862, 1121, 1430, 1479
BseBI CCWGG 3 cut(s) 561, 765, 1233
BseCI ATCGAT 2 cut(s) 404, 1174
BseDI CCNNGG 4 cut(s) 1058, 1187, 1231, 1464
BseGI GGATG 2 cut(s) 802, 1152
BseLI CCNNNNNNNGG 5 cut(s) 368, 677, 680, 949, 1048
BseMII CTCAG 5 cut(s) 178, 455, 789, 1122, 1232
BseNI ACTGG 6 cut(s) 207, 400, 862, 1121, 1430, 1479
BseRI GAGGAG 1 cut(s) 720
BseSI GKGCMC 1 cut(s) 1429
BseXI GCAGC 1 cut(s) 92
BseYI CCCAGC 2 cut(s) 1042, 1391
BshFI GGCC 2 cut(s) 788, 1307
BshNI GGYRCC 1 cut(s) 203
BshVI ATCGAT 2 cut(s) 404, 1174
BsiHKAI GWGCWC 1 cut(s) 149
BsiHKCI CYCGRG 2 cut(s) 379, 912
BslFI GGGAC 2 cut(s) 293, 965
BslI CCNNNNNNNGG 5 cut(s) 368, 677, 680, 949, 1048
BsmAI GTCTC 2 cut(s) 54, 1013
BsmFI GGGAC 2 cut(s) 293, 965
BsmI GAATGC 1 cut(s) 188
BsnI GGCC 2 cut(s) 788, 1307
BsoBI CYCGRG 2 cut(s) 379, 912
Bsp1286I GDGCHC 2 cut(s) 149, 1429
Bsp1407I TGTACA 1 cut(s) 975
Bsp19I CCATGG 1 cut(s) 1058
BspACI CCGC 1 cut(s) 74
BspANI GGCC 2 cut(s) 788, 1307
BspCNI CTCAG 5 cut(s) 177, 454, 788, 1123, 1233
BspDI ATCGAT 2 cut(s) 404, 1174
BspLI GGNNCC 5 cut(s) 205, 309, 729, 769, 941
BspMAI CTGCAG 2 cut(s) 128, 233
BspMI ACCTGC 3 cut(s) 236, 526, 1349
BspPI GGATC 7 cut(s) 146, 551, 691, 762, 775, 976, 1403
BspT107I GGYRCC 1 cut(s) 203
BspTI CTTAAG 1 cut(s) 593
BsrGI TGTACA 1 cut(s) 975
BsrI ACTGG 6 cut(s) 207, 400, 862, 1121, 1430, 1479
BssECI CCNNGG 4 cut(s) 1058, 1187, 1231, 1464
BssNAI GTATAC 1 cut(s) 1221
BssSI CACGAG 1 cut(s) 732
BssT1I CCWWGG 3 cut(s) 1058, 1187, 1464
Bst1107I GTATAC 1 cut(s) 1221
Bst2BI CACGAG 1 cut(s) 732
Bst2UI CCWGG 3 cut(s) 561, 765, 1233
Bst4CI ACNGT 2 cut(s) 366, 1424
BstAFI CTTAAG 1 cut(s) 593
BstAUI TGTACA 1 cut(s) 975
BstC8I GCNNGC 4 cut(s) 103, 1291, 1437, 1456
BstDEI CTNAG 5 cut(s) 164, 441, 775, 1131, 1241
BstDSI CCRYGG 1 cut(s) 1058
BstENI CCTNNNNNAGG 1 cut(s) 678
BstF5I GGATG 2 cut(s) 802, 1152
BstMAI GTCTC 2 cut(s) 54, 1013
BstMWI GCNNNNNNNGC 2 cut(s) 114, 123
BstNI CCWGG 3 cut(s) 561, 765, 1233
BstNSI RCATGY 1 cut(s) 508
BstSCI CCNGG 3 cut(s) 559, 763, 1231
BstSFI CTRYAG 4 cut(s) 96, 124, 229, 1420
BstSLI GKGCMC 1 cut(s) 1429
BstV1I GCAGC 1 cut(s) 92
BstV2I GAAGAC 1 cut(s) 820
BstX2I RGATCY 2 cut(s) 683, 767
BstYI RGATCY 2 cut(s) 683, 767
BstZ17I GTATAC 1 cut(s) 1221
Bsu15I ATCGAT 2 cut(s) 404, 1174
BsuRI GGCC 2 cut(s) 788, 1307
BsuTUI ATCGAT 2 cut(s) 404, 1174
BtgI CCRYGG 1 cut(s) 1058
BtsCI GGATG 2 cut(s) 802, 1152
BtsI GCAGTG 2 cut(s) 238, 585
BveI ACCTGC 3 cut(s) 236, 526, 1349
Cac8I GCNNGC 4 cut(s) 103, 1291, 1437, 1456
Cfr13I GGNCC 3 cut(s) 307, 1119, 1306
ClaI ATCGAT 2 cut(s) 404, 1174
CseI GACGC 1 cut(s) 1072
Csp6I GTAC 4 cut(s) 63, 204, 449, 976
CviQI GTAC 4 cut(s) 63, 204, 449, 976
DdeI CTNAG 5 cut(s) 164, 441, 775, 1131, 1241
Eco130I CCWWGG 3 cut(s) 1058, 1187, 1464
Eco47I GGWCC 2 cut(s) 307, 1119
Eco57I CTGAAG 1 cut(s) 1149
Eco88I CYCGRG 2 cut(s) 379, 912
EcoNI CCTNNNNNAGG 1 cut(s) 678
EcoO109I RGGNCCY 1 cut(s) 307
EcoRI GAATTC 1 cut(s) 278
EcoRII CCWGG 3 cut(s) 559, 763, 1231
EcoT14I CCWWGG 3 cut(s) 1058, 1187, 1464
EcoT22I ATGCAT 1 cut(s) 1367
ErhI CCWWGG 3 cut(s) 1058, 1187, 1464
FalI AAGNNNNNCTT 1 cut(s) 1480
FaqI GGGAC 2 cut(s) 293, 965
FauNDI CATATG 1 cut(s) 1405
FbaI TGATCA 1 cut(s) 1408
FblI GTMKAC 2 cut(s) 819, 1220
Fnu4HI GCNGC 1 cut(s) 106
FokI GGATG 2 cut(s) 809, 1159
Fsp4HI GCNGC 1 cut(s) 106
FspBI CTAG 1 cut(s) 606
GluI GCNGC 1 cut(s) 106
GsaI CCCAGC 2 cut(s) 1046, 1395
HaeIII GGCC 2 cut(s) 788, 1307
HgaI GACGC 1 cut(s) 1072
HincII GTYRAC 1 cut(s) 1264
HindII GTYRAC 1 cut(s) 1264
HinfI GANTC 8 cut(s) 389, 632, 654, 737, 821, 962, 989, 1236
HphI GGTGA 3 cut(s) 308, 507, 881
Hpy166II GTNNAC 5 cut(s) 515, 820, 1087, 1221, 1264
Hpy188I TCNGA 6 cut(s) 198, 266, 339, 688, 811, 1132
Hpy188III TCNNGA 9 cut(s) 155, 379, 734, 912, 937, 966, 1093, 1240, 1497
Hpy8I GTNNAC 5 cut(s) 515, 820, 1087, 1221, 1264
HpyAV CCTTC 3 cut(s) 172, 609, 896
HpyCH4III ACNGT 2 cut(s) 366, 1424
HpyF10VI GCNNNNNNNGC 2 cut(s) 114, 123
HpyF3I CTNAG 5 cut(s) 164, 441, 775, 1131, 1241
KpnI GGTACC 1 cut(s) 207
Ksp22I TGATCA 1 cut(s) 1408
LmnI GCTCC 2 cut(s) 733, 1349
Lsp1109I GCAGC 1 cut(s) 92
MaeI CTAG 1 cut(s) 606
MaeIII GTNAC 2 cut(s) 481, 790
MboII GAAGA 4 cut(s) 187, 334, 732, 820
MflI RGATCY 2 cut(s) 683, 767
MhlI GDGCHC 2 cut(s) 149, 1429
MlyI GAGTC 3 cut(s) 383, 815, 956
MmeI TCCRAC 2 cut(s) 623, 985
Mph1103I ATGCAT 1 cut(s) 1367
MroXI GAANNNNTTC 1 cut(s) 631
MseI TTAA 4 cut(s) 242, 258, 413, 594
MslI CAYNNNNRTG 1 cut(s) 1094
MspCI CTTAAG 1 cut(s) 593
MspR9I CCNGG 3 cut(s) 561, 765, 1233
Mva1269I GAATGC 1 cut(s) 188
MvaI CCWGG 3 cut(s) 561, 765, 1233
MwoI GCNNNNNNNGC 2 cut(s) 114, 123
NcoI CCATGG 1 cut(s) 1058
NdeI CATATG 1 cut(s) 1405
NlaIV GGNNCC 5 cut(s) 205, 309, 729, 769, 941
NmuCI GTSAC 1 cut(s) 790
NsiI ATGCAT 1 cut(s) 1367
NspI RCATGY 1 cut(s) 508
PaeR7I CTCGAG 2 cut(s) 379, 912
PaqCI CACCTGC 2 cut(s) 236, 526
PctI GAATGC 1 cut(s) 188
PdmI GAANNNNTTC 1 cut(s) 631
PfeI GAWTC 5 cut(s) 632, 654, 737, 989, 1236
PflFI GACNNNGTC 1 cut(s) 824
PflMI CCANNNNNTGG 1 cut(s) 368
PkrI GCNGC 1 cut(s) 107
PleI GAGTC 3 cut(s) 383, 815, 956
PpsI GAGTC 3 cut(s) 383, 815, 956
PpuMI RGGWCCY 1 cut(s) 307
PsiI TTATAA 1 cut(s) 1353
Psp5II RGGWCCY 1 cut(s) 307
Psp6I CCWGG 3 cut(s) 559, 763, 1231
PspFI CCCAGC 2 cut(s) 1042, 1391
PspGI CCWGG 3 cut(s) 559, 763, 1231
PspN4I GGNNCC 5 cut(s) 205, 309, 729, 769, 941
PspPI GGNCC 3 cut(s) 307, 1119, 1306
PspPPI RGGWCCY 1 cut(s) 307
PstI CTGCAG 2 cut(s) 128, 233
PsuI RGATCY 2 cut(s) 683, 767
PsyI GACNNNGTC 1 cut(s) 824
RsaI GTAC 4 cut(s) 64, 205, 450, 977
RsaNI GTAC 4 cut(s) 63, 204, 449, 976
RseI CAYNNNNRTG 1 cut(s) 1094
SaqAI TTAA 4 cut(s) 242, 258, 413, 594
SatI GCNGC 1 cut(s) 106
Sau96I GGNCC 3 cut(s) 307, 1119, 1306
SchI GAGTC 3 cut(s) 383, 815, 956
ScrFI CCNGG 3 cut(s) 561, 765, 1233
SduI GDGCHC 2 cut(s) 149, 1429
SfcI CTRYAG 4 cut(s) 96, 124, 229, 1420
Sfr274I CTCGAG 2 cut(s) 379, 912
SinI GGWCC 2 cut(s) 307, 1119
SlaI CTCGAG 2 cut(s) 379, 912
SmiMI CAYNNNNRTG 1 cut(s) 1094
SmlI CTYRAG 4 cut(s) 109, 379, 593, 912
SmoI CTYRAG 4 cut(s) 109, 379, 593, 912
SsiI CCGC 1 cut(s) 74
SspMI CTAG 1 cut(s) 606
StyD4I CCNGG 3 cut(s) 559, 763, 1231
StyI CCWWGG 3 cut(s) 1058, 1187, 1464
TaaI ACNGT 2 cut(s) 366, 1424
TaqI TCGA 7 cut(s) 380, 404, 652, 747, 913, 1174, 1398
TatI WGTACW 3 cut(s) 62, 448, 975
TfiI GAWTC 5 cut(s) 632, 654, 737, 989, 1236
Tru1I TTAA 4 cut(s) 242, 258, 413, 594
Tru9I TTAA 4 cut(s) 242, 258, 413, 594
TseFI GTSAC 1 cut(s) 790
TseI GCWGC 1 cut(s) 105
Tsp45I GTSAC 1 cut(s) 790
TspGWI ACGGA 1 cut(s) 809
Tth111I GACNNNGTC 1 cut(s) 824
Van91I CCANNNNNTGG 1 cut(s) 368
Vha464I CTTAAG 1 cut(s) 593
VpaK11BI GGWCC 2 cut(s) 307, 1119
XagI CCTNNNNNAGG 1 cut(s) 678
XapI RAATTY 8 cut(s) 10, 278, 299, 341, 698, 805, 1126, 1256
XceI RCATGY 1 cut(s) 508
XcmI CCANNNNNNNNNTGG 1 cut(s) 1447
XhoI CTCGAG 2 cut(s) 379, 912
XmiI GTMKAC 2 cut(s) 819, 1220
XmnI GAANNNNTTC 1 cut(s) 631
XspI CTAG 1 cut(s) 606
Zsp2I ATGCAT 1 cut(s) 1367
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.