Rmu_sc0003541.1_g000056

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003541.1
OG0002404
Physical Location & Seq
Forward (+)
266981 .. 269539
2559 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003541.1_g000056.1.cds

Sequence Viewer

Length: 681 bp
ctgcgttcagctctttctctcttgctcttccctgaaaccctagcgctctcatccttcctctcatgctttctttccacggctactgtctctccctcattgaactactgcctccgtcccctcaccggcgccatcgcctctgccggtgcttctcttccaacgccaccacgtcgttgctcagagatccaaaccccaactggtccaacctgcctccaaggagtttatccttctcgattggtgcgacttcgagcaccaaatgccagcgacggaggaatcgaacacaaccgcaattacgacggtgcaaccagcatcagggactatatagactctggatcagtgtacattcttgcagattcaggtggggaagttggagttccctatgagacttactttccaatgccaaccccaacaaaggataaaccatggtattccatagaacaaggaagcgtccacatcacgatgatttcaacagagcatgactggaccaaaaattctgagcagtatcaatggatgaaaagggacatggcttcagttgatcgatctgaaactccttggttgatatttatggggcatagaccaatgtatacatctgcccctggattcctgagtgttgatgagaaattcgttgacaaagttgaaccattacttctggcaagcaaagtaacatcccagaccttcccctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003993 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0044237
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

226

Amino Acids

24.94

Weight (kDa)

5.81

Isoelectric Point (pI)

53.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 212
AccB1I GGYRCC 1 cut(s) 125
AccI GTMKAC 1 cut(s) 581
AciI CCGC 1 cut(s) 283
AclWI GGATC 2 cut(s) 175, 337
AcsI RAATTY 2 cut(s) 487, 617
AcuI CTGAAG 1 cut(s) 510
AcyI GRCGYC 1 cut(s) 126
AfaI GTAC 1 cut(s) 338
AfeI AGCGCT 1 cut(s) 45
AfiI CCNNNNNNNGG 3 cut(s) 122, 309, 409
AgsI TTSAA 3 cut(s) 100, 465, 635
AjiI CACGTC 1 cut(s) 167
AjnI CCWGG 1 cut(s) 592
AluBI AGCT 1 cut(s) 11
AluI AGCT 1 cut(s) 11
Alw21I GWGCWC 1 cut(s) 250
Alw26I GTCTC 2 cut(s) 91, 374
AlwI GGATC 2 cut(s) 175, 337
Aor51HI AGCGCT 1 cut(s) 45
ApoI RAATTY 2 cut(s) 487, 617
AspLEI GCGC 2 cut(s) 46, 128
AspS9I GGNCC 2 cut(s) 197, 480
AsuHPI GGTGA 1 cut(s) 112
AvaII GGWCC 2 cut(s) 197, 480
BanI GGYRCC 1 cut(s) 125
Bbv12I GWGCWC 1 cut(s) 250
BccI CCATC 1 cut(s) 137
BceAI ACGGC 1 cut(s) 93
BciT130I CCWGG 1 cut(s) 594
BcoDI GTCTC 2 cut(s) 91, 374
BfaI CTAG 2 cut(s) 41, 679
BfoI RGCGCY 2 cut(s) 47, 129
BfuAI ACCTGC 1 cut(s) 212
Bme1390I CCNGG 1 cut(s) 594
Bme18I GGWCC 2 cut(s) 197, 480
BmgBI CACGTC 1 cut(s) 167
BmgT120I GGNCC 2 cut(s) 197, 480
BmiI GGNNCC 1 cut(s) 127
BmrFI CCNGG 1 cut(s) 594
BmsI GCATC 1 cut(s) 315
Bsa29I ATCGAT 1 cut(s) 535
BsaHI GRCGYC 1 cut(s) 126
BsaJI CCNNGG 5 cut(s) 75, 211, 419, 548, 592
BsaXI ACNNNNNCTCC 2 cut(s) 73, 103
Bsc4I CCNNNNNNNGG 3 cut(s) 122, 309, 409
Bse118I RCCGGY 2 cut(s) 122, 140
Bse1I ACTGG 2 cut(s) 199, 482
BseBI CCWGG 1 cut(s) 594
BseCI ATCGAT 1 cut(s) 535
BseDI CCNNGG 5 cut(s) 75, 211, 419, 548, 592
BseGI GGATG 3 cut(s) 50, 513, 662
BseLI CCNNNNNNNGG 3 cut(s) 122, 309, 409
BseMII CTCAG 3 cut(s) 189, 483, 593
BseNI ACTGG 2 cut(s) 199, 482
BshNI GGYRCC 1 cut(s) 125
BshVI ATCGAT 1 cut(s) 535
BsiHKAI GWGCWC 1 cut(s) 250
BsiSI CCGG 2 cut(s) 123, 141
BslFI GGGAC 3 cut(s) 99, 326, 530
BslI CCNNNNNNNGG 3 cut(s) 122, 309, 409
BsmAI GTCTC 2 cut(s) 91, 374
BsmFI GGGAC 3 cut(s) 99, 326, 530
Bsp1286I GDGCHC 1 cut(s) 250
Bsp1407I TGTACA 1 cut(s) 336
Bsp143I GATC 4 cut(s) 180, 329, 532, 536
Bsp19I CCATGG 1 cut(s) 419
BspACI CCGC 1 cut(s) 283
BspCNI CTCAG 3 cut(s) 188, 484, 594
BspDI ATCGAT 1 cut(s) 535
BspLI GGNNCC 1 cut(s) 127
BspMI ACCTGC 1 cut(s) 212
BspPI GGATC 2 cut(s) 175, 337
BspQI GCTCTTC 1 cut(s) 32
BspT107I GGYRCC 1 cut(s) 125
BsrFI RCCGGY 2 cut(s) 122, 140
BsrGI TGTACA 1 cut(s) 336
BsrI ACTGG 2 cut(s) 199, 482
BssAI RCCGGY 2 cut(s) 122, 140
BssECI CCNNGG 5 cut(s) 75, 211, 419, 548, 592
BssMI GATC 4 cut(s) 180, 329, 532, 536
BssNAI GTATAC 1 cut(s) 582
BssNI GRCGYC 1 cut(s) 126
BssT1I CCWWGG 3 cut(s) 211, 419, 548
Bst1107I GTATAC 1 cut(s) 582
Bst2UI CCWGG 1 cut(s) 594
Bst4CI ACNGT 2 cut(s) 85, 296
Bst6I CTCTTC 2 cut(s) 32, 156
BstACI GRCGYC 1 cut(s) 126
BstAPI GCANNNNNTGC 1 cut(s) 254
BstAUI TGTACA 1 cut(s) 336
BstC8I GCNNGC 2 cut(s) 259, 652
BstDEI CTNAG 3 cut(s) 175, 492, 602
BstDSI CCRYGG 2 cut(s) 75, 419
BstF5I GGATG 3 cut(s) 50, 513, 662
BstH2I RGCGCY 2 cut(s) 47, 129
BstHHI GCGC 2 cut(s) 46, 128
BstKTI GATC 4 cut(s) 183, 332, 535, 539
BstMAI GTCTC 2 cut(s) 91, 374
BstMBI GATC 4 cut(s) 180, 329, 532, 536
BstMWI GCNNNNNNNGC 1 cut(s) 254
BstNI CCWGG 1 cut(s) 594
BstSCI CCNGG 1 cut(s) 592
BstX2I RGATCY 1 cut(s) 180
BstYI RGATCY 1 cut(s) 180
BstZ17I GTATAC 1 cut(s) 582
Bsu15I ATCGAT 1 cut(s) 535
BsuTUI ATCGAT 1 cut(s) 535
BtgI CCRYGG 2 cut(s) 75, 419
BtgZI GCGATG 1 cut(s) 115
BtrI CACGTC 1 cut(s) 167
BtsCI GGATG 3 cut(s) 50, 513, 662
BtsIMutI CAGTG 1 cut(s) 339
BveI ACCTGC 1 cut(s) 212
Cac8I GCNNGC 2 cut(s) 259, 652
CfoI GCGC 2 cut(s) 46, 128
Cfr10I RCCGGY 2 cut(s) 122, 140
Cfr13I GGNCC 2 cut(s) 197, 480
ClaI ATCGAT 1 cut(s) 535
CseI GACGC 1 cut(s) 433
Csp6I GTAC 1 cut(s) 337
CviAII CATG 4 cut(s) 63, 420, 473, 520
CviJI RGCY 3 cut(s) 11, 80, 524
CviKI_1 RGCY 3 cut(s) 11, 80, 524
CviQI GTAC 1 cut(s) 337
DdeI CTNAG 3 cut(s) 175, 492, 602
DinI GGCGCC 1 cut(s) 127
DpnI GATC 4 cut(s) 182, 331, 534, 538
DpnII GATC 4 cut(s) 180, 329, 532, 536
Eam1104I CTCTTC 2 cut(s) 32, 156
EarI CTCTTC 2 cut(s) 32, 156
Eco130I CCWWGG 3 cut(s) 211, 419, 548
Eco47I GGWCC 2 cut(s) 197, 480
Eco47III AGCGCT 1 cut(s) 45
Eco57I CTGAAG 1 cut(s) 510
EcoRII CCWGG 1 cut(s) 592
EcoT14I CCWWGG 3 cut(s) 211, 419, 548
EgeI GGCGCC 1 cut(s) 127
EheI GGCGCC 1 cut(s) 127
ErhI CCWWGG 3 cut(s) 211, 419, 548
FaeI CATG 4 cut(s) 66, 423, 476, 523
FaqI GGGAC 3 cut(s) 99, 326, 530
FatI CATG 4 cut(s) 62, 419, 472, 519
FblI GTMKAC 1 cut(s) 581
FokI GGATG 3 cut(s) 37, 520, 649
FspBI CTAG 2 cut(s) 41, 679
GlaI GCGC 2 cut(s) 45, 127
HaeII RGCGCY 2 cut(s) 47, 129
HapII CCGG 2 cut(s) 123, 141
HgaI GACGC 1 cut(s) 433
HhaI GCGC 2 cut(s) 46, 128
Hin1I GRCGYC 1 cut(s) 126
Hin1II CATG 4 cut(s) 66, 423, 476, 523
Hin6I GCGC 2 cut(s) 44, 126
HinP1I GCGC 2 cut(s) 44, 126
HincII GTYRAC 1 cut(s) 625
HindII GTYRAC 1 cut(s) 625
HinfI GANTC 4 cut(s) 270, 323, 350, 597
HpaII CCGG 2 cut(s) 123, 141
HphI GGTGA 1 cut(s) 112
Hpy166II GTNNAC 4 cut(s) 337, 448, 582, 625
Hpy188I TCNGA 3 cut(s) 178, 493, 541
Hpy188III TCNNGA 4 cut(s) 228, 327, 454, 601
Hpy8I GTNNAC 4 cut(s) 337, 448, 582, 625
Hpy99I CGWCG 3 cut(s) 171, 266, 296
HpyAV CCTTC 2 cut(s) 64, 234
HpyCH4III ACNGT 2 cut(s) 85, 296
HpyCH4IV ACGT 1 cut(s) 166
HpyCH4V TGCA 2 cut(s) 299, 347
HpyF10VI GCNNNNNNNGC 1 cut(s) 254
HpyF3I CTNAG 3 cut(s) 175, 492, 602
HpySE526I ACGT 1 cut(s) 166
Hsp92I GRCGYC 1 cut(s) 126
Hsp92II CATG 4 cut(s) 66, 423, 476, 523
HspAI GCGC 2 cut(s) 44, 126
KasI GGCGCC 1 cut(s) 125
Kzo9I GATC 4 cut(s) 180, 329, 532, 536
LguI GCTCTTC 1 cut(s) 32
LweI GCATC 1 cut(s) 315
MaeI CTAG 2 cut(s) 41, 679
MaeII ACGT 1 cut(s) 166
MaeIII GTNAC 1 cut(s) 658
MalI GATC 4 cut(s) 182, 331, 534, 538
MboI GATC 4 cut(s) 180, 329, 532, 536
MboII GAAGA 2 cut(s) 19, 143
MflI RGATCY 1 cut(s) 180
MhlI GDGCHC 1 cut(s) 250
MluCI AATT 3 cut(s) 286, 487, 617
Mly113I GGCGCC 1 cut(s) 126
MlyI GAGTC 1 cut(s) 317
MmeI TCCRAC 3 cut(s) 179, 224, 346
MnlI CCTC 7 cut(s) 68, 103, 119, 128, 145, 218, 260
MslI CAYNNNNRTG 1 cut(s) 455
MspI CCGG 2 cut(s) 123, 141
MspR9I CCNGG 1 cut(s) 594
MvaI CCWGG 1 cut(s) 594
MwoI GCNNNNNNNGC 1 cut(s) 254
NarI GGCGCC 1 cut(s) 126
NcoI CCATGG 1 cut(s) 419
NdeII GATC 4 cut(s) 180, 329, 532, 536
NlaIII CATG 4 cut(s) 66, 423, 476, 523
NlaIV GGNNCC 1 cut(s) 127
PciSI GCTCTTC 1 cut(s) 32
PcsI WCGNNNNNNNCGW 2 cut(s) 235, 270
PfeI GAWTC 3 cut(s) 270, 350, 597
PleI GAGTC 1 cut(s) 317
PluTI GGCGCC 1 cut(s) 129
PpsI GAGTC 1 cut(s) 317
Psp6I CCWGG 1 cut(s) 592
PspGI CCWGG 1 cut(s) 592
PspN4I GGNNCC 1 cut(s) 127
PspPI GGNCC 2 cut(s) 197, 480
PsuI RGATCY 1 cut(s) 180
RsaI GTAC 1 cut(s) 338
RsaNI GTAC 1 cut(s) 337
RseI CAYNNNNRTG 1 cut(s) 455
SapI GCTCTTC 1 cut(s) 32
Sau3AI GATC 4 cut(s) 180, 329, 532, 536
Sau96I GGNCC 2 cut(s) 197, 480
SchI GAGTC 1 cut(s) 317
ScrFI CCNGG 1 cut(s) 594
SduI GDGCHC 1 cut(s) 250
SetI ASST 5 cut(s) 13, 169, 206, 358, 674
SfaNI GCATC 1 cut(s) 315
SfoI GGCGCC 1 cut(s) 127
SgrAI CRCCGGYG 1 cut(s) 122
SinI GGWCC 2 cut(s) 197, 480
SmiMI CAYNNNNRTG 1 cut(s) 455
Sse9I AATT 3 cut(s) 286, 487, 617
SsiI CCGC 1 cut(s) 283
SspDI GGCGCC 1 cut(s) 125
SspMI CTAG 2 cut(s) 41, 679
StyD4I CCNGG 1 cut(s) 592
StyI CCWWGG 3 cut(s) 211, 419, 548
TaaI ACNGT 2 cut(s) 85, 296
TaiI ACGT 1 cut(s) 169
TaqI TCGA 4 cut(s) 229, 244, 273, 535
TasI AATT 3 cut(s) 286, 487, 617
TatI WGTACW 1 cut(s) 336
TfiI GAWTC 3 cut(s) 270, 350, 597
TscAI CASTG 1 cut(s) 339
TspDTI ATGAA 1 cut(s) 524
TspGWI ACGGA 2 cut(s) 101, 279
TspRI CASTG 1 cut(s) 339
VpaK11BI GGWCC 2 cut(s) 197, 480
XapI RAATTY 2 cut(s) 487, 617
XcmI CCANNNNNNNNNTGG 1 cut(s) 191
XmiI GTMKAC 1 cut(s) 581
XspI CTAG 2 cut(s) 41, 679
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.