Rroxscaffold_4G00328290

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0002404
Physical Location & Seq
Forward (+)
61194621 .. 61200787
6167 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00328290.1

Sequence Viewer

Length: 1920 bp
ATGGGTTCTTCCGGCACTTGTGGGGTTCCCAAACTTATCTGTGTTGTGTTCGTGATATTGTTTCTTGGCTCCTGTGCTTCTTCATCATCTTCCTTGCACCCTTTGGTTGTCCAGTCACAGATTGAACACCTTAACCACACGGCCATATCAGAGTTTAGGTTGCTGAACAGAAAATTTCTTGGCAACTGTCCGAATCCGAGCCCATATCTGCAAATTAGTGTTAACTCGAGTCCCGGTGGCCTTGGAGATGATGAGTTTGTGACTGTCAATGTTAGTGGCGTCTTGAACCCTTCTAAAAATGATTGGGTGGCCATGATTTCACCTGCTAATTCTGATGTCTCAACCTGTCCTTTGAATACGTTTTACTATGTACAGACTGGTGATTTTAGTAAACTTCCACTTCTCTGCCATTATCCTGTTAAGGCAACATACATGAGCACTGATCCTGACTACCTCAGTTGCAAGAAGAAGGAATGCAAAAAGTATCAGAATGGTACCAGTCACTGTGCTATCTCCACCTGCAGTGGTTCGATAACATTTCATGTTATTAACATCAGAACCGACATCGAATTCGTCTTGTTTTCTGATGGATTTGAGGTCCCTTGCATTTTGAAGAAAGCAAACCCTCTGAAATTTGCCAATCCAAAGAAGCCACTGTATGGACATCTCTCGAGCATAGACTCAACTGGAACATCGATGAAATTAACATGGGTCAGTGGAGATGAGAAACCTCAGCAAGTACAATATGGAGATGGAAAATCACAGACTTCAGTAGTTACCACATTTTCACAAGATGACATGCAGAGTTTACCTGCTATACCAAGTCCAGCCAAAGACTTTGGGTGGCATGATCCAGGCTTCATTCATTCAGCAGTGATGACAGGACTTGAGCCTTCATCTAGCTTCTCTTATAGATATGGAAGTGATTCAGTTGGTTGGAGTGATCGAATCCAATTTCGCACTCCCCCTGTGGGAGGATCTGATGAACTCAAATTTCTTGCATTTGGCGACATGGGAAAGGCTCCTCGTGATTCTTCTGTAGAGCATTATATTCAGCCAGGATCCCTCTCAGTGATTGAAGCCGTGACAGATGAAATAAATTCCGGGAATGTAGACTCTGTCTTCCACATTGGAGATATAAGCTATGCCACTGGGTTTTTAGTTGAATGGGATTTTTTCCTTCACCAAATTAACCCTGTTGCTTCTCGAGTTTCATACATGACAGCTATCGGGAACCATGAGAGGGACTATATAGACTCTGGATCAGTGTACATTCTTGCAGATTCAGGTGGGGAAGTTGGAGTTCCCTATGAGACTTACTTTCCAATGCCAACCCCAGCAAAGGATAAACCATGGTATTCCATAGAACAAGGCAGCGTCCACATCACGATGATTTCAACAGAGCATGACTGGACCAAAAATTCTGAACAGTATCAATGGATGAAAAAGGACATGGCTTCAGTTGATCGATCTAAAACTCCTTGGTTGATATTTATGGGGCATAGACCAATGTATACATCTGCCCCTGGATTCCTGAGTGTTGATGAAAAATTCGTTGACGAAGTTGAACCATTACTACTGGCAAGCAAAGTTGATCTGGCCCTGTTTGGTCATGTCCATAACTACGAGCGAACCTGTTCCATTTATAAGAGCCAATGCATGAAATTGCCTAGCAAAGATGAGGCTGGGATAGACACATATGATCACAGCAACTACAGTGCGCCAGTGCAAGCTATAATTGGAATGGCTGGCTTTTCCTTGGACAAGTTTCCCAGTGGGATTAACAATAGTTGGAGTTTATCAAGGCATTCCGAATATGGTTATCTCAGAGCGCATGCAACAAGGACAGATATCAAATTAGAGTTTGTGAATGCAAACACAAGAAAAGTTGACGACAGCTTCCACATTACAAAAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003993 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0044237
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

639

Amino Acids

70.62

Weight (kDa)

5.74

Isoelectric Point (pI)

41.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
fn3_PAP PF17808 83 - 214 4.1e-35 Fn3-like domain from Purple Acid Phosphatase
Pur_ac_phosph_N PF16656 220 - 321 8.9e-16 Purple acid Phosphatase, N-terminal domain
Metallophos PF00149 330 - 542 1.1e-16 Calcineurin-like phosphoesterase
Metallophos_C PF14008 574 - 633 5.7e-10 Iron/zinc purple acid phosphatase-like protein C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1647
AarI CACCTGC 2 cut(s) 331, 527
Acc36I ACCTGC 3 cut(s) 331, 527, 820
Acc65I GGTACC 1 cut(s) 494
AccB1I GGYRCC 1 cut(s) 494
AccB7I CCANNNNNTGG 1 cut(s) 659
AccI GTMKAC 2 cut(s) 1113, 1514
AclWI GGATC 6 cut(s) 437, 845, 985, 1056, 1069, 1270
AcoI YGGCCR 2 cut(s) 141, 309
AcsI RAATTY 7 cut(s) 173, 569, 632, 992, 1099, 1420, 1550
AcuI CTGAAG 2 cut(s) 753, 1443
AcyI GRCGYC 1 cut(s) 279
AfaI GTAC 4 cut(s) 372, 496, 741, 1271
AfiI CCNNNNNNNGG 5 cut(s) 659, 971, 974, 1243, 1342
AgsI TTSAA 8 cut(s) 125, 286, 355, 613, 1079, 1166, 1398, 1568
AjnI CCWGG 3 cut(s) 853, 1057, 1525
AluBI AGCT 5 cut(s) 903, 1143, 1226, 1733, 1899
AluI AGCT 5 cut(s) 903, 1143, 1226, 1733, 1899
Alw21I GWGCWC 1 cut(s) 440
Alw26I GTCTC 2 cut(s) 343, 1307
AlwI GGATC 6 cut(s) 437, 845, 985, 1056, 1069, 1270
AlwNI CAGNNNCTG 1 cut(s) 504
Ama87I CYCGRG 3 cut(s) 226, 670, 1206
AoxI GGCC 4 cut(s) 141, 238, 309, 1599
ApeKI GCWGC 1 cut(s) 1374
ApoI RAATTY 7 cut(s) 173, 569, 632, 992, 1099, 1420, 1550
ArsI GACNNNNNNTTYG 2 cut(s) 554, 586
Asp700I GAANNNNTTC 2 cut(s) 925, 1636
Asp718I GGTACC 1 cut(s) 494
AspLEI GCGC 2 cut(s) 1723, 1834
AspS9I GGNCC 3 cut(s) 598, 1413, 1600
AsuC2I CCSGG 2 cut(s) 234, 1105
AsuHPI GGTGA 3 cut(s) 312, 392, 1175
AvaI CYCGRG 3 cut(s) 226, 670, 1206
AvaII GGWCC 2 cut(s) 598, 1413
BalI TGGCCA 1 cut(s) 311
BamHI GGATCC 1 cut(s) 1061
BanI GGYRCC 1 cut(s) 494
BanII GRGCYC 1 cut(s) 203
BauI CACGAG 1 cut(s) 1026
BbsI GAAGAC 1 cut(s) 1114
Bbv12I GWGCWC 1 cut(s) 440
BbvCI CCTCAGC 1 cut(s) 732
BbvI GCAGC 1 cut(s) 1386
BccI CCATC 2 cut(s) 581, 746
BceAI ACGGC 2 cut(s) 156, 1067
BciT130I CCWGG 3 cut(s) 855, 1059, 1527
BclI TGATCA 1 cut(s) 1702
BcnI CCSGG 2 cut(s) 234, 1105
BcoDI GTCTC 2 cut(s) 343, 1307
BfaI CTAG 2 cut(s) 900, 1671
BfmI CTRYAG 3 cut(s) 520, 1038, 1714
BfuAI ACCTGC 3 cut(s) 331, 527, 820
BisI GCNGC 1 cut(s) 1375
BlsI GCNGC 1 cut(s) 1376
Bme1390I CCNGG 5 cut(s) 234, 855, 1059, 1105, 1527
Bme18I GGWCC 2 cut(s) 598, 1413
BmeT110I CYCGRG 3 cut(s) 226, 670, 1206
BmgT120I GGNCC 3 cut(s) 598, 1413, 1600
BmiI GGNNCC 7 cut(s) 27, 70, 496, 600, 1023, 1063, 1235
BmrFI CCNGG 5 cut(s) 234, 855, 1059, 1105, 1527
BmrI ACTGGG 2 cut(s) 1161, 1767
BmuI ACTGGG 2 cut(s) 1161, 1767
BpiI GAAGAC 1 cut(s) 1114
Bpu10I CCTNAGC 1 cut(s) 732
BpuEI CTTGAG 1 cut(s) 908
BpuMI CCSGG 2 cut(s) 234, 1105
Bsa29I ATCGAT 2 cut(s) 695, 1468
BsaHI GRCGYC 1 cut(s) 279
BsaJI CCNNGG 5 cut(s) 241, 1352, 1481, 1525, 1758
Bsc4I CCNNNNNNNGG 5 cut(s) 659, 971, 974, 1243, 1342
Bse1I ACTGG 9 cut(s) 112, 382, 498, 691, 1156, 1415, 1584, 1724, 1773
BseBI CCWGG 3 cut(s) 855, 1059, 1527
BseCI ATCGAT 2 cut(s) 695, 1468
BseDI CCNNGG 5 cut(s) 241, 1352, 1481, 1525, 1758
BseGI GGATG 1 cut(s) 1446
BseLI CCNNNNNNNGG 5 cut(s) 659, 971, 974, 1243, 1342
BseMII CTCAG 5 cut(s) 469, 746, 1083, 1526, 1840
BseNI ACTGG 9 cut(s) 112, 382, 498, 691, 1156, 1415, 1584, 1724, 1773
BseRI GAGGAG 1 cut(s) 1014
BseXI GCAGC 1 cut(s) 1386
BseYI CCCAGC 2 cut(s) 1336, 1685
BshFI GGCC 4 cut(s) 143, 240, 311, 1601
BshNI GGYRCC 1 cut(s) 494
BshVI ATCGAT 2 cut(s) 695, 1468
BsiHKAI GWGCWC 1 cut(s) 440
BsiHKCI CYCGRG 3 cut(s) 226, 670, 1206
BsiSI CCGG 3 cut(s) 12, 234, 1104
BslFI GGGAC 3 cut(s) 216, 584, 1259
BslI CCNNNNNNNGG 5 cut(s) 659, 971, 974, 1243, 1342
BsmAI GTCTC 2 cut(s) 343, 1307
BsmFI GGGAC 3 cut(s) 216, 584, 1259
BsmI GAATGC 3 cut(s) 479, 1807, 1876
BsnI GGCC 4 cut(s) 143, 240, 311, 1601
BsoBI CYCGRG 3 cut(s) 226, 670, 1206
Bsp1286I GDGCHC 2 cut(s) 203, 440
Bsp1407I TGTACA 2 cut(s) 370, 1269
Bsp19I CCATGG 1 cut(s) 1352
BspANI GGCC 4 cut(s) 143, 240, 311, 1601
BspCNI CTCAG 5 cut(s) 468, 745, 1082, 1527, 1839
BspDI ATCGAT 2 cut(s) 695, 1468
BspLI GGNNCC 7 cut(s) 27, 70, 496, 600, 1023, 1063, 1235
BspMAI CTGCAG 1 cut(s) 524
BspMI ACCTGC 3 cut(s) 331, 527, 820
BspPI GGATC 6 cut(s) 437, 845, 985, 1056, 1069, 1270
BspT107I GGYRCC 1 cut(s) 494
BsrGI TGTACA 2 cut(s) 370, 1269
BsrI ACTGG 9 cut(s) 112, 382, 498, 691, 1156, 1415, 1584, 1724, 1773
BssECI CCNNGG 5 cut(s) 241, 1352, 1481, 1525, 1758
BssNAI GTATAC 1 cut(s) 1515
BssNI GRCGYC 1 cut(s) 279
BssSI CACGAG 1 cut(s) 1026
BssT1I CCWWGG 4 cut(s) 241, 1352, 1481, 1758
Bst1107I GTATAC 1 cut(s) 1515
Bst2BI CACGAG 1 cut(s) 1026
Bst2UI CCWGG 3 cut(s) 855, 1059, 1527
Bst4CI ACNGT 6 cut(s) 188, 265, 506, 657, 1431, 1718
BstACI GRCGYC 1 cut(s) 279
BstAUI TGTACA 2 cut(s) 370, 1269
BstC8I GCNNGC 4 cut(s) 1585, 1731, 1750, 1836
BstDEI CTNAG 5 cut(s) 455, 732, 1069, 1535, 1826
BstDSI CCRYGG 1 cut(s) 1352
BstENI CCTNNNNNAGG 1 cut(s) 972
BstF5I GGATG 1 cut(s) 1446
BstHHI GCGC 2 cut(s) 1723, 1834
BstMAI GTCTC 2 cut(s) 343, 1307
BstNI CCWGG 3 cut(s) 855, 1059, 1527
BstNSI RCATGY 2 cut(s) 802, 1838
BstSCI CCNGG 5 cut(s) 232, 853, 1057, 1103, 1525
BstSFI CTRYAG 3 cut(s) 520, 1038, 1714
BstV1I GCAGC 1 cut(s) 1386
BstV2I GAAGAC 1 cut(s) 1114
BstX2I RGATCY 2 cut(s) 977, 1061
BstYI RGATCY 2 cut(s) 977, 1061
BstZ17I GTATAC 1 cut(s) 1515
Bsu15I ATCGAT 2 cut(s) 695, 1468
BsuRI GGCC 4 cut(s) 143, 240, 311, 1601
BsuTUI ATCGAT 2 cut(s) 695, 1468
BtgI CCRYGG 1 cut(s) 1352
BtsCI GGATG 1 cut(s) 1446
BtsI GCAGTG 2 cut(s) 529, 879
BveI ACCTGC 3 cut(s) 331, 527, 820
Cac8I GCNNGC 4 cut(s) 1585, 1731, 1750, 1836
CaiI CAGNNNCTG 1 cut(s) 504
CfoI GCGC 2 cut(s) 1723, 1834
Cfr13I GGNCC 3 cut(s) 598, 1413, 1600
ClaI ATCGAT 2 cut(s) 695, 1468
CseI GACGC 2 cut(s) 268, 1366
Csp6I GTAC 4 cut(s) 371, 495, 740, 1270
CspCI CAANNNNNGTGG 2 cut(s) 256, 291
CviQI GTAC 4 cut(s) 371, 495, 740, 1270
DdeI CTNAG 5 cut(s) 455, 732, 1069, 1535, 1826
EaeI YGGCCR 2 cut(s) 141, 309
Eco130I CCWWGG 4 cut(s) 241, 1352, 1481, 1758
Eco24I GRGCYC 1 cut(s) 203
Eco32I GATATC 1 cut(s) 1852
Eco47I GGWCC 2 cut(s) 598, 1413
Eco57I CTGAAG 2 cut(s) 753, 1443
Eco88I CYCGRG 3 cut(s) 226, 670, 1206
EcoNI CCTNNNNNAGG 1 cut(s) 972
EcoO109I RGGNCCY 1 cut(s) 598
EcoRI GAATTC 1 cut(s) 569
EcoRII CCWGG 3 cut(s) 853, 1057, 1525
EcoRV GATATC 1 cut(s) 1852
EcoT14I CCWWGG 4 cut(s) 241, 1352, 1481, 1758
EcoT22I ATGCAT 1 cut(s) 1661
EcoT38I GRGCYC 1 cut(s) 203
ErhI CCWWGG 4 cut(s) 241, 1352, 1481, 1758
FaqI GGGAC 3 cut(s) 216, 584, 1259
FauNDI CATATG 1 cut(s) 1699
FbaI TGATCA 1 cut(s) 1702
FblI GTMKAC 2 cut(s) 1113, 1514
Fnu4HI GCNGC 1 cut(s) 1375
FokI GGATG 1 cut(s) 1453
FriOI GRGCYC 1 cut(s) 203
Fsp4HI GCNGC 1 cut(s) 1375
FspBI CTAG 2 cut(s) 900, 1671
GlaI GCGC 2 cut(s) 1722, 1833
GluI GCNGC 1 cut(s) 1375
GsaI CCCAGC 2 cut(s) 1340, 1689
HaeIII GGCC 4 cut(s) 143, 240, 311, 1601
HapII CCGG 3 cut(s) 12, 234, 1104
HgaI GACGC 2 cut(s) 268, 1366
HhaI GCGC 2 cut(s) 1723, 1834
Hin1I GRCGYC 1 cut(s) 279
Hin6I GCGC 2 cut(s) 1721, 1832
HinP1I GCGC 2 cut(s) 1721, 1832
HincII GTYRAC 3 cut(s) 223, 1558, 1891
HindII GTYRAC 3 cut(s) 223, 1558, 1891
HpaI GTTAAC 1 cut(s) 223
HpaII CCGG 3 cut(s) 12, 234, 1104
HphI GGTGA 3 cut(s) 312, 392, 1175
Hpy166II GTNNAC 9 cut(s) 223, 392, 809, 1114, 1270, 1381, 1515, 1558, 1891
Hpy8I GTNNAC 9 cut(s) 223, 392, 809, 1114, 1270, 1381, 1515, 1558, 1891
HpyAV CCTTC 4 cut(s) 300, 463, 903, 1190
HpyCH4III ACNGT 6 cut(s) 188, 265, 506, 657, 1431, 1718
HpyCH4IV ACGT 1 cut(s) 359
HpyF3I CTNAG 5 cut(s) 455, 732, 1069, 1535, 1826
HpySE526I ACGT 1 cut(s) 359
Hsp92I GRCGYC 1 cut(s) 279
HspAI GCGC 2 cut(s) 1721, 1832
KpnI GGTACC 1 cut(s) 498
Ksp22I TGATCA 1 cut(s) 1702
KspAI GTTAAC 1 cut(s) 223
LmnI GCTCC 2 cut(s) 74, 1027
Lsp1109I GCAGC 1 cut(s) 1386
MaeI CTAG 2 cut(s) 900, 1671
MaeII ACGT 1 cut(s) 359
MaeIII GTNAC 5 cut(s) 114, 259, 500, 775, 1084
MboII GAAGA 6 cut(s) 72, 81, 478, 625, 1026, 1114
MflI RGATCY 2 cut(s) 977, 1061
MhlI GDGCHC 2 cut(s) 203, 440
MlsI TGGCCA 1 cut(s) 311
MluNI TGGCCA 1 cut(s) 311
MlyI GAGTC 4 cut(s) 238, 674, 1109, 1250
MmeI TCCRAC 3 cut(s) 917, 1279, 1772
MnlI CCTC 9 cut(s) 464, 589, 636, 741, 968, 1035, 1076, 1236, 1675
Mox20I TGGCCA 1 cut(s) 311
Mph1103I ATGCAT 1 cut(s) 1661
MroXI GAANNNNTTC 2 cut(s) 925, 1636
MscI TGGCCA 1 cut(s) 311
MseI TTAA 7 cut(s) 132, 222, 420, 549, 704, 1191, 1782
MslI CAYNNNNRTG 1 cut(s) 1388
Msp20I TGGCCA 1 cut(s) 311
MspI CCGG 3 cut(s) 12, 234, 1104
MspR9I CCNGG 5 cut(s) 234, 855, 1059, 1105, 1527
Mva1269I GAATGC 3 cut(s) 479, 1807, 1876
MvaI CCWGG 3 cut(s) 855, 1059, 1527
NciI CCSGG 2 cut(s) 234, 1105
NcoI CCATGG 1 cut(s) 1352
NdeI CATATG 1 cut(s) 1699
NlaIV GGNNCC 7 cut(s) 27, 70, 496, 600, 1023, 1063, 1235
NmuCI GTSAC 4 cut(s) 114, 259, 500, 1084
NsiI ATGCAT 1 cut(s) 1661
NspI RCATGY 2 cut(s) 802, 1838
PaeI GCATGC 1 cut(s) 1838
PaeR7I CTCGAG 3 cut(s) 226, 670, 1206
PaqCI CACCTGC 2 cut(s) 331, 527
PctI GAATGC 3 cut(s) 479, 1807, 1876
PdmI GAANNNNTTC 2 cut(s) 925, 1636
PfeI GAWTC 6 cut(s) 193, 926, 948, 1031, 1283, 1530
PflFI GACNNNGTC 1 cut(s) 1118
PflMI CCANNNNNTGG 1 cut(s) 659
PfoI TCCNGGA 1 cut(s) 1103
PkrI GCNGC 1 cut(s) 1376
PleI GAGTC 4 cut(s) 237, 674, 1109, 1250
PpsI GAGTC 4 cut(s) 237, 674, 1109, 1250
PpuMI RGGWCCY 1 cut(s) 598
PsiI TTATAA 1 cut(s) 1647
Psp5II RGGWCCY 1 cut(s) 598
Psp6I CCWGG 3 cut(s) 853, 1057, 1525
PspFI CCCAGC 2 cut(s) 1336, 1685
PspGI CCWGG 3 cut(s) 853, 1057, 1525
PspN4I GGNNCC 7 cut(s) 27, 70, 496, 600, 1023, 1063, 1235
PspPI GGNCC 3 cut(s) 598, 1413, 1600
PspPPI RGGWCCY 1 cut(s) 598
PspXI VCTCGAGB 1 cut(s) 226
PsrI GAACNNNNNNTAC 2 cut(s) 1560, 1592
PstI CTGCAG 1 cut(s) 524
PstNI CAGNNNCTG 1 cut(s) 504
PsuI RGATCY 2 cut(s) 977, 1061
PsyI GACNNNGTC 1 cut(s) 1118
RsaI GTAC 4 cut(s) 372, 496, 741, 1271
RsaNI GTAC 4 cut(s) 371, 495, 740, 1270
RseI CAYNNNNRTG 1 cut(s) 1388
SaqAI TTAA 7 cut(s) 132, 222, 420, 549, 704, 1191, 1782
SatI GCNGC 1 cut(s) 1375
Sau96I GGNCC 3 cut(s) 598, 1413, 1600
SchI GAGTC 4 cut(s) 238, 674, 1109, 1250
ScrFI CCNGG 5 cut(s) 234, 855, 1059, 1105, 1527
SduI GDGCHC 2 cut(s) 203, 440
SfcI CTRYAG 3 cut(s) 520, 1038, 1714
Sfr274I CTCGAG 3 cut(s) 226, 670, 1206
SinI GGWCC 2 cut(s) 598, 1413
SlaI CTCGAG 3 cut(s) 226, 670, 1206
SmiMI CAYNNNNRTG 1 cut(s) 1388
SmlI CTYRAG 4 cut(s) 226, 670, 887, 1206
SmoI CTYRAG 4 cut(s) 226, 670, 887, 1206
SphI GCATGC 1 cut(s) 1838
SspMI CTAG 2 cut(s) 900, 1671
StyD4I CCNGG 5 cut(s) 232, 853, 1057, 1103, 1525
StyI CCWWGG 4 cut(s) 241, 1352, 1481, 1758
TaaI ACNGT 6 cut(s) 188, 265, 506, 657, 1431, 1718
TaiI ACGT 1 cut(s) 362
TaqI TCGA 8 cut(s) 227, 530, 567, 671, 695, 946, 1207, 1468
TatI WGTACW 3 cut(s) 370, 739, 1269
TfiI GAWTC 6 cut(s) 193, 926, 948, 1031, 1283, 1530
Tru1I TTAA 7 cut(s) 132, 222, 420, 549, 704, 1191, 1782
Tru9I TTAA 7 cut(s) 132, 222, 420, 549, 704, 1191, 1782
TseFI GTSAC 4 cut(s) 114, 259, 500, 1084
TseI GCWGC 1 cut(s) 1374
Tsp45I GTSAC 4 cut(s) 114, 259, 500, 1084
Tth111I GACNNNGTC 1 cut(s) 1118
Van91I CCANNNNNTGG 1 cut(s) 659
VpaK11BI GGWCC 2 cut(s) 598, 1413
XagI CCTNNNNNAGG 1 cut(s) 972
XapI RAATTY 7 cut(s) 173, 569, 632, 992, 1099, 1420, 1550
XceI RCATGY 2 cut(s) 802, 1838
XhoI CTCGAG 3 cut(s) 226, 670, 1206
XmiI GTMKAC 2 cut(s) 1113, 1514
XmnI GAANNNNTTC 2 cut(s) 925, 1636
XspI CTAG 2 cut(s) 900, 1671
Zsp2I ATGCAT 1 cut(s) 1661
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.