Rmu_sc0004345.1_g000025

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004345.1
OG0002404
Physical Location & Seq
Reverse (-)
91026 .. 94281
3256 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004345.1_g000025.1.cds

Sequence Viewer

Length: 936 bp
atgtcattagacatcaccactattaaatcggtcaggattgcacctgatgttaaaaatcatgttaacatcactctttctctctcgctcttctctgaaaccctagcgctctcctccctctcctgctctctttccacagctactgtctctccctcattgaactactgcctccgtcccctcaccggcgccatcgcctctgccggtgcttctcttccaacgccaccaagtcgttcctcagagatccgaaccccaactggtccaacctgcatccaaggagtttatccttctcgattggtgcgacttcgagcaccaaatgccaacgacggaggaaccaaacacaaccgcaattacgacggtgcaaccagtatcagggactatatagaatctggatcagtgtacattcttgcagattcaggtggggaagttggagttccctatgagacttactttccctatgagacttactttccaatgccagccccagcaaaggataaaccatggtattccacagaacaaggaagcgtccacatcacgatgatttcaacagagcatgactggaccaaaaattctgagcagtatcaatggatgaaaagggacatggcttcagttgatcgatctgaaactccttggttgatatttatggggcatagaccaatgtatacatctgcccctggattcctgagtgttgatgagaaattcgttgacaaagttgaaccattacttctggcaagcaaagttgatctggcgctgtttggtcatgtccataactacgagcgaacctgctccatttataagagccaatgcatgaaattgccttgcaaagatgaggctgggatcgacacatatgatcacagcaactacagtgccccagtgccagccataattggaatggctggcttttccttggacaagtttcccagtggtgtaagtccttcttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003993 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0044237
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

311

Amino Acids

34.12

Weight (kDa)

6.06

Isoelectric Point (pI)

49.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 789
Acc36I ACCTGC 2 cut(s) 269, 785
AccB1I GGYRCC 1 cut(s) 182
AccI GTMKAC 1 cut(s) 656
AciI CCGC 1 cut(s) 340
AclWI GGATC 3 cut(s) 232, 394, 839
AcsI RAATTY 2 cut(s) 562, 692
AcuI CTGAAG 1 cut(s) 585
AcyI GRCGYC 1 cut(s) 183
AfaI GTAC 1 cut(s) 395
AfeI AGCGCT 1 cut(s) 105
AfiI CCNNNNNNNGG 3 cut(s) 179, 366, 484
AgsI TTSAA 3 cut(s) 157, 540, 710
AjnI CCWGG 1 cut(s) 667
AluBI AGCT 1 cut(s) 137
AluI AGCT 1 cut(s) 137
Alw21I GWGCWC 1 cut(s) 307
Alw26I GTCTC 3 cut(s) 148, 431, 449
AlwI GGATC 3 cut(s) 232, 394, 839
AlwNI CAGNNNCTG 1 cut(s) 140
Aor51HI AGCGCT 1 cut(s) 105
ApoI RAATTY 2 cut(s) 562, 692
AspLEI GCGC 3 cut(s) 106, 185, 745
AspS9I GGNCC 2 cut(s) 254, 555
AsuHPI GGTGA 2 cut(s) 7, 169
AvaII GGWCC 2 cut(s) 254, 555
BaeGI GKGCMC 1 cut(s) 865
BanI GGYRCC 1 cut(s) 182
Bbv12I GWGCWC 1 cut(s) 307
BccI CCATC 1 cut(s) 194
BciT130I CCWGG 1 cut(s) 669
BclI TGATCA 1 cut(s) 844
BcoDI GTCTC 3 cut(s) 148, 431, 449
BfaI CTAG 1 cut(s) 101
BfmI CTRYAG 1 cut(s) 856
BfoI RGCGCY 3 cut(s) 107, 186, 746
BfuAI ACCTGC 2 cut(s) 269, 785
Bme1390I CCNGG 1 cut(s) 669
Bme18I GGWCC 2 cut(s) 254, 555
BmgT120I GGNCC 2 cut(s) 254, 555
BmiI GGNNCC 2 cut(s) 184, 328
BmrFI CCNGG 1 cut(s) 669
BmrI ACTGGG 2 cut(s) 860, 909
BmsI GCATC 1 cut(s) 273
BmuI ACTGGG 2 cut(s) 860, 909
Bsa29I ATCGAT 1 cut(s) 610
BsaHI GRCGYC 1 cut(s) 183
BsaJI CCNNGG 5 cut(s) 268, 494, 623, 667, 900
BsaXI ACNNNNNCTCC 2 cut(s) 130, 160
Bsc4I CCNNNNNNNGG 3 cut(s) 179, 366, 484
Bse118I RCCGGY 2 cut(s) 179, 197
Bse1I ACTGG 5 cut(s) 256, 360, 557, 866, 915
BseBI CCWGG 1 cut(s) 669
BseCI ATCGAT 1 cut(s) 610
BseDI CCNNGG 5 cut(s) 268, 494, 623, 667, 900
BseGI GGATG 2 cut(s) 264, 588
BseLI CCNNNNNNNGG 3 cut(s) 179, 366, 484
BseMII CTCAG 3 cut(s) 246, 558, 668
BseNI ACTGG 5 cut(s) 256, 360, 557, 866, 915
BseRI GAGGAG 1 cut(s) 100
BseSI GKGCMC 1 cut(s) 865
BseYI CCCAGC 2 cut(s) 478, 827
BshNI GGYRCC 1 cut(s) 182
BshVI ATCGAT 1 cut(s) 610
BsiHKAI GWGCWC 1 cut(s) 307
BsiSI CCGG 2 cut(s) 180, 198
BslFI GGGAC 3 cut(s) 156, 383, 605
BslI CCNNNNNNNGG 3 cut(s) 179, 366, 484
BsmAI GTCTC 3 cut(s) 148, 431, 449
BsmFI GGGAC 3 cut(s) 156, 383, 605
Bsp1286I GDGCHC 2 cut(s) 307, 865
Bsp1407I TGTACA 1 cut(s) 393
Bsp143I GATC 7 cut(s) 237, 386, 607, 611, 736, 831, 844
Bsp19I CCATGG 1 cut(s) 494
BspACI CCGC 1 cut(s) 340
BspCNI CTCAG 3 cut(s) 245, 559, 669
BspDI ATCGAT 1 cut(s) 610
BspLI GGNNCC 2 cut(s) 184, 328
BspMI ACCTGC 2 cut(s) 269, 785
BspPI GGATC 3 cut(s) 232, 394, 839
BspQI GCTCTTC 1 cut(s) 92
BspT107I GGYRCC 1 cut(s) 182
BsrFI RCCGGY 2 cut(s) 179, 197
BsrGI TGTACA 1 cut(s) 393
BsrI ACTGG 5 cut(s) 256, 360, 557, 866, 915
BssAI RCCGGY 2 cut(s) 179, 197
BssECI CCNNGG 5 cut(s) 268, 494, 623, 667, 900
BssMI GATC 7 cut(s) 237, 386, 607, 611, 736, 831, 844
BssNAI GTATAC 1 cut(s) 657
BssNI GRCGYC 1 cut(s) 183
BssT1I CCWWGG 4 cut(s) 268, 494, 623, 900
Bst1107I GTATAC 1 cut(s) 657
Bst2UI CCWGG 1 cut(s) 669
Bst4CI ACNGT 3 cut(s) 142, 353, 860
Bst6I CTCTTC 2 cut(s) 92, 213
BstACI GRCGYC 1 cut(s) 183
BstAPI GCANNNNNTGC 1 cut(s) 311
BstAUI TGTACA 1 cut(s) 393
BstC8I GCNNGC 4 cut(s) 474, 727, 873, 892
BstDEI CTNAG 3 cut(s) 232, 567, 677
BstDSI CCRYGG 1 cut(s) 494
BstF5I GGATG 2 cut(s) 264, 588
BstH2I RGCGCY 3 cut(s) 107, 186, 746
BstHHI GCGC 3 cut(s) 106, 185, 745
BstKTI GATC 7 cut(s) 240, 389, 610, 614, 739, 834, 847
BstMAI GTCTC 3 cut(s) 148, 431, 449
BstMBI GATC 7 cut(s) 237, 386, 607, 611, 736, 831, 844
BstMWI GCNNNNNNNGC 1 cut(s) 311
BstNI CCWGG 1 cut(s) 669
BstSCI CCNGG 1 cut(s) 667
BstSFI CTRYAG 1 cut(s) 856
BstSLI GKGCMC 1 cut(s) 865
BstX2I RGATCY 1 cut(s) 237
BstYI RGATCY 1 cut(s) 237
BstZ17I GTATAC 1 cut(s) 657
Bsu15I ATCGAT 1 cut(s) 610
BsuTUI ATCGAT 1 cut(s) 610
BtgI CCRYGG 1 cut(s) 494
BtgZI GCGATG 1 cut(s) 172
BtsCI GGATG 2 cut(s) 264, 588
BtsIMutI CAGTG 4 cut(s) 396, 865, 873, 922
BveI ACCTGC 2 cut(s) 269, 785
Cac8I GCNNGC 4 cut(s) 474, 727, 873, 892
CaiI CAGNNNCTG 1 cut(s) 140
CfoI GCGC 3 cut(s) 106, 185, 745
Cfr10I RCCGGY 2 cut(s) 179, 197
Cfr13I GGNCC 2 cut(s) 254, 555
ClaI ATCGAT 1 cut(s) 610
CseI GACGC 1 cut(s) 508
Csp6I GTAC 1 cut(s) 394
CviAII CATG 6 cut(s) 59, 495, 548, 595, 755, 802
CviJI RGCY 8 cut(s) 137, 476, 599, 795, 827, 875, 890, 894
CviKI_1 RGCY 8 cut(s) 137, 476, 599, 795, 827, 875, 890, 894
CviQI GTAC 1 cut(s) 394
DdeI CTNAG 3 cut(s) 232, 567, 677
DinI GGCGCC 1 cut(s) 184
DpnI GATC 7 cut(s) 239, 388, 609, 613, 738, 833, 846
DpnII GATC 7 cut(s) 237, 386, 607, 611, 736, 831, 844
Eam1104I CTCTTC 2 cut(s) 92, 213
EarI CTCTTC 2 cut(s) 92, 213
Eco130I CCWWGG 4 cut(s) 268, 494, 623, 900
Eco47I GGWCC 2 cut(s) 254, 555
Eco47III AGCGCT 1 cut(s) 105
Eco57I CTGAAG 1 cut(s) 585
EcoRII CCWGG 1 cut(s) 667
EcoT14I CCWWGG 4 cut(s) 268, 494, 623, 900
EcoT22I ATGCAT 1 cut(s) 803
EgeI GGCGCC 1 cut(s) 184
EheI GGCGCC 1 cut(s) 184
ErhI CCWWGG 4 cut(s) 268, 494, 623, 900
FaeI CATG 6 cut(s) 62, 498, 551, 598, 758, 805
FalI AAGNNNNNCTT 1 cut(s) 916
FaqI GGGAC 3 cut(s) 156, 383, 605
FatI CATG 6 cut(s) 58, 494, 547, 594, 754, 801
FauNDI CATATG 1 cut(s) 841
FbaI TGATCA 1 cut(s) 844
FblI GTMKAC 1 cut(s) 656
FokI GGATG 2 cut(s) 251, 595
FspBI CTAG 1 cut(s) 101
GlaI GCGC 3 cut(s) 105, 184, 744
GsaI CCCAGC 2 cut(s) 482, 831
HaeII RGCGCY 3 cut(s) 107, 186, 746
HapII CCGG 2 cut(s) 180, 198
HgaI GACGC 1 cut(s) 508
HhaI GCGC 3 cut(s) 106, 185, 745
Hin1I GRCGYC 1 cut(s) 183
Hin1II CATG 6 cut(s) 62, 498, 551, 598, 758, 805
Hin6I GCGC 3 cut(s) 104, 183, 743
HinP1I GCGC 3 cut(s) 104, 183, 743
HincII GTYRAC 2 cut(s) 64, 700
HindII GTYRAC 2 cut(s) 64, 700
HinfI GANTC 3 cut(s) 380, 407, 672
HpaI GTTAAC 1 cut(s) 64
HpaII CCGG 2 cut(s) 180, 198
HphI GGTGA 2 cut(s) 7, 169
Hpy166II GTNNAC 5 cut(s) 64, 394, 523, 657, 700
Hpy188I TCNGA 5 cut(s) 94, 235, 242, 568, 616
Hpy188III TCNNGA 6 cut(s) 34, 285, 384, 529, 676, 933
Hpy8I GTNNAC 5 cut(s) 64, 394, 523, 657, 700
Hpy99I CGWCG 2 cut(s) 323, 353
HpyAV CCTTC 1 cut(s) 291
HpyCH4III ACNGT 3 cut(s) 142, 353, 860
HpyCH4V TGCA 6 cut(s) 41, 264, 356, 404, 801, 816
HpyF10VI GCNNNNNNNGC 1 cut(s) 311
HpyF3I CTNAG 3 cut(s) 232, 567, 677
Hsp92I GRCGYC 1 cut(s) 183
Hsp92II CATG 6 cut(s) 62, 498, 551, 598, 758, 805
HspAI GCGC 3 cut(s) 104, 183, 743
KasI GGCGCC 1 cut(s) 182
Ksp22I TGATCA 1 cut(s) 844
KspAI GTTAAC 1 cut(s) 64
Kzo9I GATC 7 cut(s) 237, 386, 607, 611, 736, 831, 844
LguI GCTCTTC 1 cut(s) 92
LmnI GCTCC 1 cut(s) 785
LweI GCATC 1 cut(s) 273
MaeI CTAG 1 cut(s) 101
MalI GATC 7 cut(s) 239, 388, 609, 613, 738, 833, 846
MboI GATC 7 cut(s) 237, 386, 607, 611, 736, 831, 844
MboII GAAGA 2 cut(s) 79, 200
MflI RGATCY 1 cut(s) 237
MhlI GDGCHC 2 cut(s) 307, 865
MluCI AATT 5 cut(s) 343, 562, 692, 806, 879
Mly113I GGCGCC 1 cut(s) 183
MmeI TCCRAC 3 cut(s) 236, 281, 403
MnlI CCTC 9 cut(s) 121, 125, 160, 176, 185, 202, 241, 317, 817
Mph1103I ATGCAT 1 cut(s) 803
MseI TTAA 3 cut(s) 24, 51, 63
MslI CAYNNNNRTG 1 cut(s) 530
MspI CCGG 2 cut(s) 180, 198
MspR9I CCNGG 1 cut(s) 669
MvaI CCWGG 1 cut(s) 669
MwoI GCNNNNNNNGC 1 cut(s) 311
NarI GGCGCC 1 cut(s) 183
NcoI CCATGG 1 cut(s) 494
NdeI CATATG 1 cut(s) 841
NdeII GATC 7 cut(s) 237, 386, 607, 611, 736, 831, 844
NlaIII CATG 6 cut(s) 62, 498, 551, 598, 758, 805
NlaIV GGNNCC 2 cut(s) 184, 328
NsiI ATGCAT 1 cut(s) 803
PciSI GCTCTTC 1 cut(s) 92
PcsI WCGNNNNNNNCGW 1 cut(s) 292
PfeI GAWTC 3 cut(s) 380, 407, 672
PluTI GGCGCC 1 cut(s) 186
PsiI TTATAA 1 cut(s) 789
Psp6I CCWGG 1 cut(s) 667
PspFI CCCAGC 2 cut(s) 478, 827
PspGI CCWGG 1 cut(s) 667
PspN4I GGNNCC 2 cut(s) 184, 328
PspPI GGNCC 2 cut(s) 254, 555
PstNI CAGNNNCTG 1 cut(s) 140
PsuI RGATCY 1 cut(s) 237
RsaI GTAC 1 cut(s) 395
RsaNI GTAC 1 cut(s) 394
RseI CAYNNNNRTG 1 cut(s) 530
SapI GCTCTTC 1 cut(s) 92
SaqAI TTAA 3 cut(s) 24, 51, 63
Sau3AI GATC 7 cut(s) 237, 386, 607, 611, 736, 831, 844
Sau96I GGNCC 2 cut(s) 254, 555
ScrFI CCNGG 1 cut(s) 669
SduI GDGCHC 2 cut(s) 307, 865
SetI ASST 5 cut(s) 46, 139, 263, 415, 779
SfaNI GCATC 1 cut(s) 273
SfcI CTRYAG 1 cut(s) 856
SfoI GGCGCC 1 cut(s) 184
SgrAI CRCCGGYG 1 cut(s) 179
SinI GGWCC 2 cut(s) 254, 555
SmiMI CAYNNNNRTG 1 cut(s) 530
Sse9I AATT 5 cut(s) 343, 562, 692, 806, 879
SsiI CCGC 1 cut(s) 340
SspDI GGCGCC 1 cut(s) 182
SspMI CTAG 1 cut(s) 101
StyD4I CCNGG 1 cut(s) 667
StyI CCWWGG 4 cut(s) 268, 494, 623, 900
TaaI ACNGT 3 cut(s) 142, 353, 860
TaqI TCGA 4 cut(s) 286, 301, 610, 834
TaqII GACCGA 1 cut(s) 19
TasI AATT 5 cut(s) 343, 562, 692, 806, 879
TatI WGTACW 1 cut(s) 393
TfiI GAWTC 3 cut(s) 380, 407, 672
Tru1I TTAA 3 cut(s) 24, 51, 63
Tru9I TTAA 3 cut(s) 24, 51, 63
TscAI CASTG 4 cut(s) 396, 865, 873, 922
TspDTI ATGAA 2 cut(s) 599, 818
TspGWI ACGGA 2 cut(s) 158, 336
TspRI CASTG 4 cut(s) 396, 865, 873, 922
VpaK11BI GGWCC 2 cut(s) 254, 555
XapI RAATTY 2 cut(s) 562, 692
XcmI CCANNNNNNNNNTGG 1 cut(s) 883
XmiI GTMKAC 1 cut(s) 656
XspI CTAG 1 cut(s) 101
Zsp2I ATGCAT 1 cut(s) 803
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.