pycom07g02130

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0002404
Physical Location & Seq
Reverse (-)
1727076 .. 1729912
2837 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g02130.6

Sequence Viewer

Length: 1104 bp
ATGGAGGGGGACTATATAGACACTGGATCAATCTATATTTTACCAGATTCAGGTGGAGAATCTGGAGTTCCTTATGAGACTTACTTTCCTATGCCAACCCCAGCAAAGGATAAGCCATGGTATTCCATAGAACAAGCAAGTGTTCACATTACGGTGATTTCAACAGAGCATGACTGGTCCACAAATTCCGAGCAGTATCAATGGATGAGAAAGGACATGGCTTCGTTTGATCGATCTAAAACTCCTTGGTTGATTTTTATGGGGCATAGACCCATGTATTCCTCCTCACCTGGATTCCTCAATGTTGATAAAATATTTGTCGACGAAGTGGAACCATTACTGCTCGAGAGCAAAGTTGATTTAGTCCTATTTGGTCATGTTCATAACTACGAGCGAACTTGTGCGGTTTATAAGAGTGAATGCAAAGGATTGCCTCAAAAAGATGAAAATGGGATTGACACATATGATCATAGCAACTACAGTGCCCCAGTGCACGCTGTAATTGGTATGGCTGGCTTTACCTTGGACGGGTTCTCAAGTGGTCCGGTTAATAATCCTTGGAGTTTATCCAAGATTTCTGAATTTGGTTATCTAAGAGGACATGCGACAAAGAAAGAGATGAAATTAGAGCTTGTGAATGCAGATACAAGAAAGGTTGAAGACAGATTCCGCATTACAAAATACGCAGACAGCGATAAGCGGCAGTGCGAAAGGAAGACGGCTAGCTTTCAGTTCAACTCAGCCAAGTTCCAGAAACTAACAGTGGATTCATTGTTTGAGCTGGTTCACGCTCGGCTGTTACCTGTTTTAGAGGACTCCATCAAACACTTTGCCCCAATCGACCTCCAAGACGTTCTTATGAGGTTGACTTTTGACAATGTTTGCATGATCACATTTGTGGTCGATCCCGGTTGCTTGCAGCCAGGCCATGACGTACTTCAAATTGGGCGCAGAGAGGAAGCTTATTCAGATGTCCATAATATAGGAGTGGATGAATTCGCAGACAATGTCATCCGGTCATTAACGAAAGAGAGCCAACGCCAAGCTTTTCGAAGGCCAAGTCATCTTGGTCGATCGGTATTCAATCACTGGAGTCAACATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003993 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0044237
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

368

Amino Acids

41.96

Weight (kDa)

5.75

Isoelectric Point (pI)

45.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 411
AccI GTMKAC 1 cut(s) 321
AciI CCGC 3 cut(s) 404, 670, 700
AclWI GGATC 2 cut(s) 34, 899
AcsI RAATTY 3 cut(s) 184, 581, 995
AfaI GTAC 1 cut(s) 936
AfiI CCNNNNNNNGG 3 cut(s) 50, 106, 528
AgsI TTSAA 5 cut(s) 162, 659, 736, 941, 1084
AjnI CCWGG 2 cut(s) 289, 922
AleI CACNNNNGTG 1 cut(s) 896
AluBI AGCT 5 cut(s) 631, 726, 781, 962, 1046
AluI AGCT 5 cut(s) 631, 726, 781, 962, 1046
Alw21I GWGCWC 1 cut(s) 495
Alw26I GTCTC 1 cut(s) 71
Alw44I GTGCAC 1 cut(s) 491
AlwI GGATC 2 cut(s) 34, 899
Ama87I CYCGRG 1 cut(s) 344
AoxI GGCC 2 cut(s) 925, 1055
ApaLI GTGCAC 1 cut(s) 491
ApeKI GCWGC 1 cut(s) 919
ApoI RAATTY 3 cut(s) 184, 581, 995
ArsI GACNNNNNNTTYG 2 cut(s) 1045, 1077
AspLEI GCGC 1 cut(s) 951
AspS9I GGNCC 2 cut(s) 177, 542
AsuC2I CCSGG 1 cut(s) 909
AsuHPI GGTGA 2 cut(s) 166, 279
AsuII TTCGAA 1 cut(s) 1051
AsuNHI GCTAGC 1 cut(s) 722
AvaI CYCGRG 1 cut(s) 344
AvaII GGWCC 2 cut(s) 177, 542
BaeGI GKGCMC 2 cut(s) 487, 495
BbsI GAAGAC 2 cut(s) 666, 722
Bbv12I GWGCWC 1 cut(s) 495
BbvI GCAGC 1 cut(s) 931
BccI CCATC 1 cut(s) 827
BceAI ACGGC 1 cut(s) 735
BciT130I CCWGG 2 cut(s) 291, 924
BclI TGATCA 2 cut(s) 466, 888
BcnI CCSGG 1 cut(s) 909
BcoDI GTCTC 1 cut(s) 71
BfaI CTAG 1 cut(s) 723
BfmI CTRYAG 1 cut(s) 478
BisI GCNGC 2 cut(s) 701, 920
BlsI GCNGC 2 cut(s) 702, 921
Bme1390I CCNGG 3 cut(s) 291, 909, 924
Bme18I GGWCC 2 cut(s) 177, 542
BmeT110I CYCGRG 1 cut(s) 344
BmgT120I GGNCC 2 cut(s) 177, 542
BmiI GGNNCC 1 cut(s) 333
BmrFI CCNGG 3 cut(s) 291, 909, 924
BmrI ACTGGG 1 cut(s) 482
BmtI GCTAGC 1 cut(s) 726
BmuI ACTGGG 1 cut(s) 482
BpiI GAAGAC 2 cut(s) 666, 722
BpmI CTGGAG 1 cut(s) 84
Bpu14I TTCGAA 1 cut(s) 1051
BpuEI CTTGAG 1 cut(s) 520
BpuMI CCSGG 1 cut(s) 909
Bsa29I ATCGAT 1 cut(s) 232
BsaJI CCNNGG 4 cut(s) 116, 245, 522, 557
BsaWI WCCGGW 2 cut(s) 544, 1014
Bsc4I CCNNNNNNNGG 3 cut(s) 50, 106, 528
Bse1I ACTGG 4 cut(s) 28, 179, 488, 1094
BseBI CCWGG 2 cut(s) 291, 924
BseCI ATCGAT 1 cut(s) 232
BseDI CCNNGG 4 cut(s) 116, 245, 522, 557
BseGI GGATG 3 cut(s) 210, 997, 1011
BseLI CCNNNNNNNGG 3 cut(s) 50, 106, 528
BseMII CTCAG 1 cut(s) 753
BseNI ACTGG 4 cut(s) 28, 179, 488, 1094
BseRI GAGGAG 1 cut(s) 274
BseSI GKGCMC 2 cut(s) 487, 495
BseXI GCAGC 1 cut(s) 931
BseYI CCCAGC 1 cut(s) 100
Bsh1285I CGRYCG 1 cut(s) 1076
BshFI GGCC 2 cut(s) 927, 1057
BshVI ATCGAT 1 cut(s) 232
BsiEI CGRYCG 1 cut(s) 1076
BsiHKAI GWGCWC 1 cut(s) 495
BsiHKCI CYCGRG 1 cut(s) 344
BsiSI CCGG 3 cut(s) 545, 909, 1015
BslFI GGGAC 1 cut(s) 23
BslI CCNNNNNNNGG 3 cut(s) 50, 106, 528
BsmAI GTCTC 1 cut(s) 71
BsmFI GGGAC 1 cut(s) 23
BsmI GAATGC 2 cut(s) 425, 643
BsnI GGCC 2 cut(s) 927, 1057
BsoBI CYCGRG 1 cut(s) 344
Bsp119I TTCGAA 1 cut(s) 1051
Bsp1286I GDGCHC 2 cut(s) 487, 495
Bsp143I GATC 7 cut(s) 26, 229, 233, 466, 888, 904, 1073
Bsp19I CCATGG 1 cut(s) 116
BspACI CCGC 3 cut(s) 404, 670, 700
BspANI GGCC 2 cut(s) 927, 1057
BspCNI CTCAG 1 cut(s) 752
BspDI ATCGAT 1 cut(s) 232
BspLI GGNNCC 1 cut(s) 333
BspOI GCTAGC 1 cut(s) 726
BspPI GGATC 2 cut(s) 34, 899
BspT104I TTCGAA 1 cut(s) 1051
BsrI ACTGG 4 cut(s) 28, 179, 488, 1094
BssECI CCNNGG 4 cut(s) 116, 245, 522, 557
BssMI GATC 7 cut(s) 26, 229, 233, 466, 888, 904, 1073
BssT1I CCWWGG 4 cut(s) 116, 245, 522, 557
Bst2UI CCWGG 2 cut(s) 291, 924
Bst4CI ACNGT 3 cut(s) 154, 482, 763
BstBI TTCGAA 1 cut(s) 1051
BstC8I GCNNGC 4 cut(s) 495, 514, 724, 917
BstDEI CTNAG 2 cut(s) 593, 739
BstDSI CCRYGG 1 cut(s) 116
BstF5I GGATG 3 cut(s) 210, 997, 1011
BstHHI GCGC 1 cut(s) 951
BstKTI GATC 7 cut(s) 29, 232, 236, 469, 891, 907, 1076
BstMAI GTCTC 1 cut(s) 71
BstMBI GATC 7 cut(s) 26, 229, 233, 466, 888, 904, 1073
BstMCI CGRYCG 1 cut(s) 1076
BstNI CCWGG 2 cut(s) 291, 924
BstNSI RCATGY 1 cut(s) 605
BstSCI CCNGG 3 cut(s) 289, 907, 922
BstSFI CTRYAG 1 cut(s) 478
BstSLI GKGCMC 2 cut(s) 487, 495
BstV1I GCAGC 1 cut(s) 931
BstV2I GAAGAC 2 cut(s) 666, 722
Bsu15I ATCGAT 1 cut(s) 232
BsuRI GGCC 2 cut(s) 927, 1057
BsuTUI ATCGAT 1 cut(s) 232
BtgI CCRYGG 1 cut(s) 116
BtsCI GGATG 3 cut(s) 210, 997, 1011
BtsI GCAGTG 1 cut(s) 710
BtsIMutI CAGTG 6 cut(s) 21, 487, 495, 710, 768, 1087
Cac8I GCNNGC 4 cut(s) 495, 514, 724, 917
CfoI GCGC 1 cut(s) 951
Cfr13I GGNCC 2 cut(s) 177, 542
ClaI ATCGAT 1 cut(s) 232
Csp6I GTAC 1 cut(s) 935
CviAII CATG 8 cut(s) 117, 170, 217, 274, 377, 602, 886, 929
CviQI GTAC 1 cut(s) 935
DdeI CTNAG 2 cut(s) 593, 739
DpnI GATC 7 cut(s) 28, 231, 235, 468, 890, 906, 1075
DpnII GATC 7 cut(s) 26, 229, 233, 466, 888, 904, 1073
Eco130I CCWWGG 4 cut(s) 116, 245, 522, 557
Eco47I GGWCC 2 cut(s) 177, 542
Eco88I CYCGRG 1 cut(s) 344
EcoRI GAATTC 1 cut(s) 995
EcoRII CCWGG 2 cut(s) 289, 922
EcoT14I CCWWGG 4 cut(s) 116, 245, 522, 557
ErhI CCWWGG 4 cut(s) 116, 245, 522, 557
FaeI CATG 8 cut(s) 120, 173, 220, 277, 380, 605, 889, 932
FalI AAGNNNNNCTT 2 cut(s) 840, 872
FaqI GGGAC 1 cut(s) 23
FatI CATG 8 cut(s) 116, 169, 216, 273, 376, 601, 885, 928
FauNDI CATATG 1 cut(s) 463
FbaI TGATCA 2 cut(s) 466, 888
FblI GTMKAC 1 cut(s) 321
Fnu4HI GCNGC 2 cut(s) 701, 920
FokI GGATG 3 cut(s) 217, 998, 1004
Fsp4HI GCNGC 2 cut(s) 701, 920
FspBI CTAG 1 cut(s) 723
GlaI GCGC 1 cut(s) 950
GluI GCNGC 2 cut(s) 701, 920
GsaI CCCAGC 1 cut(s) 104
GsuI CTGGAG 1 cut(s) 84
HaeIII GGCC 2 cut(s) 927, 1057
HapII CCGG 3 cut(s) 545, 909, 1015
HhaI GCGC 1 cut(s) 951
Hin1II CATG 8 cut(s) 120, 173, 220, 277, 380, 605, 889, 932
Hin6I GCGC 1 cut(s) 949
HinP1I GCGC 1 cut(s) 949
HincII GTYRAC 3 cut(s) 322, 867, 1097
HindII GTYRAC 3 cut(s) 322, 867, 1097
HindIII AAGCTT 2 cut(s) 960, 1044
HinfI GANTC 7 cut(s) 47, 59, 294, 666, 767, 815, 1093
HpaII CCGG 3 cut(s) 545, 909, 1015
HphI GGTGA 2 cut(s) 166, 279
Hpy166II GTNNAC 7 cut(s) 145, 180, 322, 493, 787, 867, 1097
Hpy188I TCNGA 3 cut(s) 190, 580, 970
Hpy188III TCNNGA 3 cut(s) 63, 346, 751
Hpy8I GTNNAC 7 cut(s) 145, 180, 322, 493, 787, 867, 1097
Hpy99I CGWCG 1 cut(s) 326
HpyAV CCTTC 1 cut(s) 1047
HpyCH4III ACNGT 3 cut(s) 154, 482, 763
HpyCH4IV ACGT 2 cut(s) 852, 933
HpyCH4V TGCA 5 cut(s) 423, 493, 641, 885, 919
HpyF3I CTNAG 2 cut(s) 593, 739
HpySE526I ACGT 2 cut(s) 852, 933
Hsp92II CATG 8 cut(s) 120, 173, 220, 277, 380, 605, 889, 932
HspAI GCGC 1 cut(s) 949
Ksp22I TGATCA 2 cut(s) 466, 888
Kzo9I GATC 7 cut(s) 26, 229, 233, 466, 888, 904, 1073
Lsp1109I GCAGC 1 cut(s) 931
MaeI CTAG 1 cut(s) 723
MaeII ACGT 2 cut(s) 852, 933
MaeIII GTNAC 1 cut(s) 798
MalI GATC 7 cut(s) 28, 231, 235, 468, 890, 906, 1075
MboI GATC 7 cut(s) 26, 229, 233, 466, 888, 904, 1073
MboII GAAGA 2 cut(s) 671, 727
MhlI GDGCHC 2 cut(s) 487, 495
MluCI AATT 6 cut(s) 184, 501, 581, 623, 942, 995
MlyI GAGTC 2 cut(s) 809, 1102
MnlI CCTC 9 cut(s) 292, 295, 308, 444, 590, 805, 854, 855, 949
MseI TTAA 3 cut(s) 549, 1022, 1102
MslI CAYNNNNRTG 2 cut(s) 152, 896
MspI CCGG 3 cut(s) 545, 909, 1015
MspR9I CCNGG 3 cut(s) 291, 909, 924
Mva1269I GAATGC 2 cut(s) 425, 643
MvaI CCWGG 2 cut(s) 291, 924
NciI CCSGG 1 cut(s) 909
NcoI CCATGG 1 cut(s) 116
NdeI CATATG 1 cut(s) 463
NdeII GATC 7 cut(s) 26, 229, 233, 466, 888, 904, 1073
NheI GCTAGC 1 cut(s) 722
NlaIII CATG 8 cut(s) 120, 173, 220, 277, 380, 605, 889, 932
NlaIV GGNNCC 1 cut(s) 333
NmeAIII GCCGAG 1 cut(s) 772
NspI RCATGY 1 cut(s) 605
NspV TTCGAA 1 cut(s) 1051
OliI CACNNNNGTG 1 cut(s) 896
PaeR7I CTCGAG 1 cut(s) 344
PcsI WCGNNNNNNNCGW 1 cut(s) 690
PctI GAATGC 2 cut(s) 425, 643
PfeI GAWTC 5 cut(s) 47, 59, 294, 666, 767
PflFI GACNNNGTC 1 cut(s) 1007
PkrI GCNGC 2 cut(s) 702, 921
Ple19I CGATCG 1 cut(s) 1076
PleI GAGTC 2 cut(s) 809, 1101
PpsI GAGTC 2 cut(s) 809, 1101
PsiI TTATAA 1 cut(s) 411
Psp6I CCWGG 2 cut(s) 289, 922
PspFI CCCAGC 1 cut(s) 100
PspGI CCWGG 2 cut(s) 289, 922
PspN4I GGNNCC 1 cut(s) 333
PspPI GGNCC 2 cut(s) 177, 542
PsyI GACNNNGTC 1 cut(s) 1007
PvuI CGATCG 1 cut(s) 1076
RsaI GTAC 1 cut(s) 936
RsaNI GTAC 1 cut(s) 935
RseI CAYNNNNRTG 2 cut(s) 152, 896
SalI GTCGAC 1 cut(s) 320
SaqAI TTAA 3 cut(s) 549, 1022, 1102
SatI GCNGC 2 cut(s) 701, 920
Sau3AI GATC 7 cut(s) 26, 229, 233, 466, 888, 904, 1073
Sau96I GGNCC 2 cut(s) 177, 542
SchI GAGTC 2 cut(s) 809, 1102
ScrFI CCNGG 3 cut(s) 291, 909, 924
SduI GDGCHC 2 cut(s) 487, 495
SfcI CTRYAG 1 cut(s) 478
Sfr274I CTCGAG 1 cut(s) 344
SfuI TTCGAA 1 cut(s) 1051
SinI GGWCC 2 cut(s) 177, 542
SlaI CTCGAG 1 cut(s) 344
SmiMI CAYNNNNRTG 2 cut(s) 152, 896
SmlI CTYRAG 2 cut(s) 344, 535
SmoI CTYRAG 2 cut(s) 344, 535
Sse9I AATT 6 cut(s) 184, 501, 581, 623, 942, 995
SsiI CCGC 3 cut(s) 404, 670, 700
SspI AATATT 1 cut(s) 315
SspMI CTAG 1 cut(s) 723
StyD4I CCNGG 3 cut(s) 289, 907, 922
StyI CCWWGG 4 cut(s) 116, 245, 522, 557
TaaI ACNGT 3 cut(s) 154, 482, 763
TaiI ACGT 2 cut(s) 855, 936
TaqI TCGA 7 cut(s) 232, 321, 345, 840, 903, 1051, 1072
TasI AATT 6 cut(s) 184, 501, 581, 623, 942, 995
TauI GCSGC 1 cut(s) 703
TfiI GAWTC 5 cut(s) 47, 59, 294, 666, 767
Tru1I TTAA 3 cut(s) 549, 1022, 1102
Tru9I TTAA 3 cut(s) 549, 1022, 1102
TscAI CASTG 6 cut(s) 28, 487, 495, 710, 768, 1094
TseI GCWGC 1 cut(s) 919
TspDTI ATGAA 5 cut(s) 371, 459, 635, 759, 1008
TspRI CASTG 6 cut(s) 28, 487, 495, 710, 768, 1094
Tth111I GACNNNGTC 1 cut(s) 1007
VneI GTGCAC 1 cut(s) 491
VpaK11BI GGWCC 2 cut(s) 177, 542
XapI RAATTY 3 cut(s) 184, 581, 995
XceI RCATGY 1 cut(s) 605
XhoI CTCGAG 1 cut(s) 344
XmiI GTMKAC 1 cut(s) 321
XspI CTAG 1 cut(s) 723
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.