Rh4AG170500

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0002404
Physical Location & Seq
Forward (+)
42946027 .. 42957211
11185 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG170500.1

Sequence Viewer

Length: 1290 bp
ATGTCATATTTTCTTGTTTACTTAATTCTATATATGCTTGCTTCTACACTTTTCAGGGACTATATAGACTCTGGATCAGTGTACATTCTTGCAGATTCAGGTGGGGAAGTTGGAGTTCCCTATGAGACTTACTTTCCAATGCCAACCCCAGCAAAGGATAAACCATGGTATTCCATAGAACAAGGAAGCGTCCACATCATGATGATTTCAACAGAGCATGACTGGACCAAAAATTCTGAGCAGTATCAATGGATGAAAAAGGACATGGCTTCAGTTGATCGATCTAAAACTCCTTGGTTGATATTTATGGGGCATAGACCAATGTATACATCTGCCCCTGGATTCCTGAGTGTTGATGAGAAATTCGTTGACAAAGTTGAACCATTACTTCTGGCAAGCAAAGTTGATCTAGCGCTGTTTGGTCATGTCCATAACTACGAGCGAACCTGCTCCATTTATAAGAGCCAATACATGAAATTGCCTTGCAAAGATGAGGCTGGGATCGACACATATGATCACAGCAACTACAGTGCCCCAATGCAAGCCATAATTGGAATGGCTGGCTTTTCCTTGGACAAGTTTCCCAGTGGTGGCTCTGCTACTACAGAAAGTGCTTTTCCTCCACGCATTTCCTCAGGAATTGAAAATCTAAAATGTGGGAAAATTGCTGCTCCTTTACCACCAAAGGATACATTTATGGAGGATCCGCTACGCACGAGTTCTTCAAGCTATCCGTTTTGCGCAAGGTTCAGATTCATACTCGATGAACAACTAAAGGAAGCTTCTGCCTGTGATGAAGTGAAAGCTGCTCTATCAGCTAAAATTAGCAGAGAGCAAATTGGAGCTGAGATTGATCTGATGATCTCTGGAAACCAACCTCATTGTGTCACATACTTGGATTGTACATGGATTCTTATTCAATTAGTGGGGCACTCTACTTTCAATGACGAACAAAGAAGGCTTTCCGTTTATGCTGCAATGTTCCTTCCACTTAGGAAGACAATGTATAAAGATAGGAAAGCAAAAGATGTTCCTGTCGTGAATTATATTTTCAGAGACTCCCTTAAACAAGGAGTCAGCAATGCTGAAACTGTTATTAATTTACACAATGCATTGGAGAAGTTTTTGTCTTTGCTTCCTCACTTTGTACCAAATGGGGATGTAAAACTCGCCGAAGTTGATATGGGAAGAGAATATGTTGATGTCCCTCTTACATCGAAAGAATCGAATCTCCGAGTGTTAACAGAATCTCAAACCAGAAGATCGCAGAGCTTTGCTTGTAGAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003993 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0044237
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

429

Amino Acids

48.45

Weight (kDa)

6.09

Isoelectric Point (pI)

46.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 72 - 146 2.9e-07 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 459
Acc16I TGCGCA 1 cut(s) 742
Acc36I ACCTGC 1 cut(s) 455
AccI GTMKAC 1 cut(s) 326
AciI CCGC 1 cut(s) 707
AclWI GGATC 4 cut(s) 82, 509, 698, 711
AcsI RAATTY 2 cut(s) 232, 362
AcuI CTGAAG 1 cut(s) 255
AfaI GTAC 3 cut(s) 83, 904, 1149
AfeI AGCGCT 1 cut(s) 414
AfiI CCNNNNNNNGG 2 cut(s) 154, 590
AgsI TTSAA 6 cut(s) 210, 380, 644, 726, 920, 943
AjnI CCWGG 1 cut(s) 337
AluBI AGCT 7 cut(s) 729, 782, 806, 818, 845, 1272, 1287
AluI AGCT 7 cut(s) 729, 782, 806, 818, 845, 1272, 1287
Alw26I GTCTC 2 cut(s) 119, 1050
AlwI GGATC 4 cut(s) 82, 509, 698, 711
Aor51HI AGCGCT 1 cut(s) 414
ApeKI GCWGC 3 cut(s) 668, 806, 974
ApoI RAATTY 2 cut(s) 232, 362
AseI ATTAAT 1 cut(s) 1098
Asp700I GAANNNNTTC 1 cut(s) 961
AspLEI GCGC 2 cut(s) 415, 743
AspS9I GGNCC 1 cut(s) 225
AvaII GGWCC 1 cut(s) 225
AxyI CCTNAGG 1 cut(s) 634
BaeGI GKGCMC 2 cut(s) 535, 933
BamHI GGATCC 1 cut(s) 703
BauI CACGAG 1 cut(s) 715
BbsI GAAGAC 1 cut(s) 1004
BbvI GCAGC 3 cut(s) 655, 793, 961
BciT130I CCWGG 1 cut(s) 339
BciVI GTATCC 1 cut(s) 682
BclI TGATCA 1 cut(s) 514
BcoDI GTCTC 2 cut(s) 119, 1050
BfaI CTAG 1 cut(s) 410
BfmI CTRYAG 2 cut(s) 526, 603
BfoI RGCGCY 1 cut(s) 416
BfuAI ACCTGC 1 cut(s) 455
BfuI GTATCC 1 cut(s) 682
BisI GCNGC 3 cut(s) 669, 807, 975
BlsI GCNGC 3 cut(s) 670, 808, 976
Bme1390I CCNGG 1 cut(s) 339
Bme18I GGWCC 1 cut(s) 225
BmgT120I GGNCC 1 cut(s) 225
BmiI GGNNCC 1 cut(s) 705
BmrFI CCNGG 1 cut(s) 339
BmrI ACTGGG 1 cut(s) 579
BmuI ACTGGG 1 cut(s) 579
BpiI GAAGAC 1 cut(s) 1004
Bsa29I ATCGAT 1 cut(s) 280
BsaJI CCNNGG 4 cut(s) 164, 293, 337, 570
Bsc4I CCNNNNNNNGG 2 cut(s) 154, 590
Bse1I ACTGG 2 cut(s) 227, 585
Bse21I CCTNAGG 1 cut(s) 634
Bse3DI GCAATG 2 cut(s) 984, 1087
BseBI CCWGG 1 cut(s) 339
BseCI ATCGAT 1 cut(s) 280
BseDI CCNNGG 4 cut(s) 164, 293, 337, 570
BseGI GGATG 2 cut(s) 258, 1165
BseLI CCNNNNNNNGG 2 cut(s) 154, 590
BseMI GCAATG 2 cut(s) 984, 1087
BseMII CTCAG 4 cut(s) 228, 338, 648, 837
BseNI ACTGG 2 cut(s) 227, 585
BseSI GKGCMC 2 cut(s) 535, 933
BseXI GCAGC 3 cut(s) 655, 793, 961
BseYI CCCAGC 2 cut(s) 148, 497
BshVI ATCGAT 1 cut(s) 280
BslFI GGGAC 2 cut(s) 71, 1190
BslI CCNNNNNNNGG 2 cut(s) 154, 590
BsmAI GTCTC 2 cut(s) 119, 1050
BsmFI GGGAC 2 cut(s) 71, 1190
Bsp1286I GDGCHC 2 cut(s) 535, 933
Bsp1407I TGTACA 2 cut(s) 81, 902
Bsp19I CCATGG 1 cut(s) 164
BspACI CCGC 1 cut(s) 707
BspCNI CTCAG 4 cut(s) 229, 339, 647, 838
BspDI ATCGAT 1 cut(s) 280
BspHI TCATGA 1 cut(s) 198
BspLI GGNNCC 1 cut(s) 705
BspMI ACCTGC 1 cut(s) 455
BspPI GGATC 4 cut(s) 82, 509, 698, 711
BsrDI GCAATG 2 cut(s) 984, 1087
BsrGI TGTACA 2 cut(s) 81, 902
BsrI ACTGG 2 cut(s) 227, 585
BssECI CCNNGG 4 cut(s) 164, 293, 337, 570
BssNAI GTATAC 1 cut(s) 327
BssSI CACGAG 1 cut(s) 715
BssT1I CCWWGG 3 cut(s) 164, 293, 570
Bst1107I GTATAC 1 cut(s) 327
Bst2BI CACGAG 1 cut(s) 715
Bst2UI CCWGG 1 cut(s) 339
Bst4CI ACNGT 2 cut(s) 530, 1093
Bst6I CTCTTC 1 cut(s) 1183
BstAUI TGTACA 2 cut(s) 81, 902
BstC8I GCNNGC 4 cut(s) 39, 397, 543, 562
BstDEI CTNAG 5 cut(s) 237, 347, 634, 846, 992
BstDSI CCRYGG 1 cut(s) 164
BstF5I GGATG 2 cut(s) 258, 1165
BstH2I RGCGCY 1 cut(s) 416
BstHHI GCGC 2 cut(s) 415, 743
BstMAI GTCTC 2 cut(s) 119, 1050
BstMWI GCNNNNNNNGC 1 cut(s) 815
BstNI CCWGG 1 cut(s) 339
BstSCI CCNGG 1 cut(s) 337
BstSFI CTRYAG 2 cut(s) 526, 603
BstSLI GKGCMC 2 cut(s) 535, 933
BstV1I GCAGC 3 cut(s) 655, 793, 961
BstV2I GAAGAC 1 cut(s) 1004
BstX2I RGATCY 1 cut(s) 703
BstYI RGATCY 1 cut(s) 703
BstZ17I GTATAC 1 cut(s) 327
Bsu15I ATCGAT 1 cut(s) 280
Bsu36I CCTNAGG 1 cut(s) 634
BsuI GTATCC 1 cut(s) 682
BsuTUI ATCGAT 1 cut(s) 280
BtgI CCRYGG 1 cut(s) 164
BtsCI GGATG 2 cut(s) 258, 1165
BtsIMutI CAGTG 3 cut(s) 84, 535, 592
BveI ACCTGC 1 cut(s) 455
Cac8I GCNNGC 4 cut(s) 39, 397, 543, 562
CciI TCATGA 1 cut(s) 198
CfoI GCGC 2 cut(s) 415, 743
Cfr13I GGNCC 1 cut(s) 225
ClaI ATCGAT 1 cut(s) 280
CseI GACGC 1 cut(s) 178
Csp6I GTAC 3 cut(s) 82, 903, 1148
CviAII CATG 7 cut(s) 165, 199, 218, 265, 425, 472, 906
CviQI GTAC 3 cut(s) 82, 903, 1148
DdeI CTNAG 5 cut(s) 237, 347, 634, 846, 992
Eam1104I CTCTTC 1 cut(s) 1183
EarI CTCTTC 1 cut(s) 1183
Eco130I CCWWGG 3 cut(s) 164, 293, 570
Eco47I GGWCC 1 cut(s) 225
Eco47III AGCGCT 1 cut(s) 414
Eco57I CTGAAG 1 cut(s) 255
Eco81I CCTNAGG 1 cut(s) 634
EcoRII CCWGG 1 cut(s) 337
EcoT14I CCWWGG 3 cut(s) 164, 293, 570
EcoT22I ATGCAT 1 cut(s) 1114
ErhI CCWWGG 3 cut(s) 164, 293, 570
FaeI CATG 7 cut(s) 168, 202, 221, 268, 428, 475, 909
FaqI GGGAC 2 cut(s) 71, 1190
FatI CATG 7 cut(s) 164, 198, 217, 264, 424, 471, 905
FauNDI CATATG 1 cut(s) 511
FbaI TGATCA 1 cut(s) 514
FblI GTMKAC 1 cut(s) 326
Fnu4HI GCNGC 3 cut(s) 669, 807, 975
FokI GGATG 2 cut(s) 265, 1172
Fsp4HI GCNGC 3 cut(s) 669, 807, 975
FspBI CTAG 1 cut(s) 410
FspI TGCGCA 1 cut(s) 742
GlaI GCGC 2 cut(s) 414, 742
GluI GCNGC 3 cut(s) 669, 807, 975
GsaI CCCAGC 2 cut(s) 152, 501
HaeII RGCGCY 1 cut(s) 416
HgaI GACGC 1 cut(s) 178
HhaI GCGC 2 cut(s) 415, 743
Hin1II CATG 7 cut(s) 168, 202, 221, 268, 428, 475, 909
Hin6I GCGC 2 cut(s) 413, 741
HinP1I GCGC 2 cut(s) 413, 741
HincII GTYRAC 2 cut(s) 370, 1242
HindII GTYRAC 2 cut(s) 370, 1242
HindIII AAGCTT 1 cut(s) 780
HpaI GTTAAC 1 cut(s) 1242
Hpy166II GTNNAC 6 cut(s) 19, 82, 193, 327, 370, 1242
Hpy188I TCNGA 5 cut(s) 238, 752, 858, 1055, 1235
Hpy188III TCNNGA 6 cut(s) 72, 199, 346, 636, 867, 1039
Hpy8I GTNNAC 6 cut(s) 19, 82, 193, 327, 370, 1242
HpyAV CCTTC 2 cut(s) 951, 995
HpyCH4III ACNGT 2 cut(s) 530, 1093
HpyCH4V TGCA 5 cut(s) 92, 486, 541, 977, 1112
HpyF10VI GCNNNNNNNGC 1 cut(s) 815
HpyF3I CTNAG 5 cut(s) 237, 347, 634, 846, 992
Hsp92II CATG 7 cut(s) 168, 202, 221, 268, 428, 475, 909
HspAI GCGC 2 cut(s) 413, 741
Ksp22I TGATCA 1 cut(s) 514
KspAI GTTAAC 1 cut(s) 1242
LmnI GCTCC 3 cut(s) 455, 676, 842
Lsp1109I GCAGC 3 cut(s) 655, 793, 961
MaeI CTAG 1 cut(s) 410
MaeIII GTNAC 1 cut(s) 886
MboII GAAGA 4 cut(s) 714, 1009, 1200, 1272
MflI RGATCY 1 cut(s) 703
MhlI GDGCHC 2 cut(s) 535, 933
MlyI GAGTC 3 cut(s) 62, 1052, 1083
MmeI TCCRAC 1 cut(s) 91
MnlI CCTC 7 cut(s) 487, 630, 643, 694, 888, 1149, 1218
Mph1103I ATGCAT 1 cut(s) 1114
MroXI GAANNNNTTC 1 cut(s) 961
MseI TTAA 4 cut(s) 23, 1065, 1098, 1241
MslI CAYNNNNRTG 1 cut(s) 200
MspR9I CCNGG 1 cut(s) 339
MvaI CCWGG 1 cut(s) 339
MwoI GCNNNNNNNGC 1 cut(s) 815
NcoI CCATGG 1 cut(s) 164
NdeI CATATG 1 cut(s) 511
NlaIII CATG 7 cut(s) 168, 202, 221, 268, 428, 475, 909
NlaIV GGNNCC 1 cut(s) 705
NmuCI GTSAC 1 cut(s) 886
NsbI TGCGCA 1 cut(s) 742
NsiI ATGCAT 1 cut(s) 1114
PagI TCATGA 1 cut(s) 198
PcsI WCGNNNNNNNCGW 1 cut(s) 1223
PdmI GAANNNNTTC 1 cut(s) 961
PfeI GAWTC 7 cut(s) 95, 342, 753, 910, 1223, 1228, 1247
PkrI GCNGC 3 cut(s) 670, 808, 976
PleI GAGTC 3 cut(s) 62, 1052, 1082
PpsI GAGTC 3 cut(s) 62, 1052, 1082
PshBI ATTAAT 1 cut(s) 1098
PsiI TTATAA 1 cut(s) 459
Psp6I CCWGG 1 cut(s) 337
PspFI CCCAGC 2 cut(s) 148, 497
PspGI CCWGG 1 cut(s) 337
PspN4I GGNNCC 1 cut(s) 705
PspPI GGNCC 1 cut(s) 225
PsuI RGATCY 1 cut(s) 703
RsaI GTAC 3 cut(s) 83, 904, 1149
RsaNI GTAC 3 cut(s) 82, 903, 1148
RseI CAYNNNNRTG 1 cut(s) 200
SaqAI TTAA 4 cut(s) 23, 1065, 1098, 1241
SatI GCNGC 3 cut(s) 669, 807, 975
Sau96I GGNCC 1 cut(s) 225
SchI GAGTC 3 cut(s) 62, 1052, 1083
ScrFI CCNGG 1 cut(s) 339
SduI GDGCHC 2 cut(s) 535, 933
SfcI CTRYAG 2 cut(s) 526, 603
SinI GGWCC 1 cut(s) 225
SmiMI CAYNNNNRTG 1 cut(s) 200
SsiI CCGC 1 cut(s) 707
SspMI CTAG 1 cut(s) 410
StyD4I CCNGG 1 cut(s) 337
StyI CCWWGG 3 cut(s) 164, 293, 570
TaaI ACNGT 2 cut(s) 530, 1093
TaqI TCGA 5 cut(s) 280, 504, 762, 1217, 1226
TatI WGTACW 2 cut(s) 81, 902
TfiI GAWTC 7 cut(s) 95, 342, 753, 910, 1223, 1228, 1247
Tru1I TTAA 4 cut(s) 23, 1065, 1098, 1241
Tru9I TTAA 4 cut(s) 23, 1065, 1098, 1241
TscAI CASTG 3 cut(s) 84, 535, 592
TseFI GTSAC 1 cut(s) 886
TseI GCWGC 3 cut(s) 668, 806, 974
Tsp45I GTSAC 1 cut(s) 886
TspDTI ATGAA 5 cut(s) 269, 488, 745, 780, 810
TspGWI ACGGA 2 cut(s) 723, 955
TspRI CASTG 3 cut(s) 84, 535, 592
VpaK11BI GGWCC 1 cut(s) 225
VspI ATTAAT 1 cut(s) 1098
XapI RAATTY 2 cut(s) 232, 362
XcmI CCANNNNNNNNNTGG 1 cut(s) 553
XmiI GTMKAC 1 cut(s) 326
XmnI GAANNNNTTC 1 cut(s) 961
XspI CTAG 1 cut(s) 410
Zsp2I ATGCAT 1 cut(s) 1114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.