Rorug07G0178400

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0002404
Physical Location & Seq
Reverse (-)
14812673 .. 14814443
1771 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0178400.1

Sequence Viewer

Length: 900 bp
ATGGGTTTTCCGGCCAACGGCGCCACCGCCGACCAGCAGCTCAAGTTCCTGATCTACGGCAAGACCGGCTGGATTGGCGGCCTCCTCGGCAAGATATGCCAGGCCCAGAGCATTTGCTTCGAGTACGGCTCCGGCCGCCTCGAGAACCGCGCCTCCCTCGAAGCCGACATCGCCGCCGTCAAGCCCACCCACGTCTTCAACGCCGCCGGCGTGACCGGCCGCCCCAACGTCGACTGGTGCGAGTCCCACAAGGTCGAGACCATCCGCACCAACGTCGTCGGCACCTTGACCCTCGCGGACCTCTGCCGCGACCGCGGCCTCGTCCTCGTCAACTACGCCACCGGCTGCATTTTCGAGTACGACCAGGCTCACCCCGAAGGCTCCGGCATCGGCTTCAAGGAGGAGGACACCCCCAACTTTATCGGATCTTTCTATTCCAAGACCAAAGCCATGGTGGAGGATCTGCTCAAGAATTACGAGAATGTTTGTACTCTCCGTGTTAGAATGCCCATTTCATCGGACTTGCAGAACCCGCGAAACTTCATCACCAAGATTGCTCGGTATGATAAGGTGGTCAACATTCCAAACTCCATGACCATCTTGGACGAGCTCCTTCCCATTTCCGTGGAGATGGCAAAGAGGAATCTCACCGGAATCTGGAACTTTACAAACCCCGGGGTGGTCAGCCACAATGAGATCTTGGAGATGTACAAGCAATACATCGACCCCAGCTTCACCTGGAAGAACTTCAATCTTGAGGAGCAGGCAAAGGTGATTGTTGCTCCGAGGAGCAACAATGAGCTTGATGCGTCAAAATTGAAGAAAGAGTTCCCTGAACTTTTGTCCATCAAGGAGTCTCTCATCAAGAATGTGTTCAAGCCAAATCAAAAGACTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003993 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0044237
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

299

Amino Acids

33.45

Weight (kDa)

6.9

Isoelectric Point (pI)

24.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RmlD_sub_bind PF04321 15 - 186 9.5e-14 RmlD substrate binding domain
GDP_Man_Dehyd PF16363 43 - 184 5.9e-06 GDP-mannose 4,6 dehydratase
Epimerase PF01370 45 - 171 2e-12 NAD dependent epimerase/dehydratase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 20, 281
AccI GTMKAC 1 cut(s) 231
AccII CGCG 5 cut(s) 150, 296, 309, 315, 535
AclWI GGATC 2 cut(s) 433, 468
AcoI YGGCCR 3 cut(s) 12, 133, 217
AcyI GRCGYC 1 cut(s) 21
AfaI GTAC 4 cut(s) 125, 359, 490, 710
AfiI CCNNNNNNNGG 3 cut(s) 17, 657, 679
AgsI TTSAA 5 cut(s) 199, 397, 751, 820, 877
AjiI CACGTC 1 cut(s) 193
AjnI CCWGG 3 cut(s) 99, 363, 737
AloI GAACNNNNNNTCC 2 cut(s) 137, 169
AluBI AGCT 4 cut(s) 40, 610, 732, 802
AluI AGCT 4 cut(s) 40, 610, 732, 802
Alw21I GWGCWC 1 cut(s) 612
Alw26I GTCTC 2 cut(s) 251, 861
AlwI GGATC 2 cut(s) 433, 468
Ama87I CYCGRG 2 cut(s) 140, 674
AoxI GGCC 6 cut(s) 12, 79, 102, 133, 217, 316
ApeKI GCWGC 2 cut(s) 37, 345
Asp700I GAANNNNTTC 3 cut(s) 746, 827, 872
AspLEI GCGC 2 cut(s) 23, 152
AspS9I GGNCC 2 cut(s) 103, 298
AsuC2I CCSGG 2 cut(s) 675, 676
AsuHPI GGTGA 5 cut(s) 362, 538, 640, 727, 784
AvaI CYCGRG 2 cut(s) 140, 674
AvaII GGWCC 1 cut(s) 298
BanI GGYRCC 2 cut(s) 20, 281
BanII GRGCYC 1 cut(s) 612
BbsI GAAGAC 1 cut(s) 187
Bbv12I GWGCWC 1 cut(s) 612
BbvI GCAGC 2 cut(s) 49, 332
BccI CCATC 4 cut(s) 269, 605, 625, 854
BceAI ACGGC 4 cut(s) 34, 73, 142, 161
BcgI CGANNNNNNTGC 4 cut(s) 100, 134, 256, 290
BciT130I CCWGG 3 cut(s) 101, 365, 739
BcnI CCSGG 2 cut(s) 675, 676
BcoDI GTCTC 2 cut(s) 251, 861
BfoI RGCGCY 1 cut(s) 24
BglI GCCNNNNNGGC 1 cut(s) 87
BglII AGATCT 1 cut(s) 696
BisI GCNGC 9 cut(s) 38, 79, 136, 174, 204, 220, 307, 316, 346
BlsI GCNGC 9 cut(s) 39, 80, 137, 175, 205, 221, 308, 317, 347
Bme1390I CCNGG 5 cut(s) 101, 365, 675, 676, 739
Bme18I GGWCC 1 cut(s) 298
BmeT110I CYCGRG 2 cut(s) 140, 674
BmgBI CACGTC 1 cut(s) 193
BmgT120I GGNCC 2 cut(s) 103, 298
BmiI GGNNCC 4 cut(s) 22, 130, 283, 382
BmrFI CCNGG 5 cut(s) 101, 365, 675, 676, 739
BmsI GCATC 2 cut(s) 396, 796
BpiI GAAGAC 1 cut(s) 187
BplI GAGNNNNNCTC 2 cut(s) 113, 145
BpuEI CTTGAG 3 cut(s) 26, 452, 776
BpuMI CCSGG 2 cut(s) 675, 676
BsaHI GRCGYC 1 cut(s) 21
BsaI GGTCTC 1 cut(s) 251
BsaJI CCNNGG 8 cut(s) 85, 313, 450, 624, 673, 674, 675, 785
BsaWI WCCGGW 1 cut(s) 650
BsaXI ACNNNNNCTCC 2 cut(s) 137, 167
Bsc4I CCNNNNNNNGG 3 cut(s) 17, 657, 679
Bse118I RCCGGY 4 cut(s) 65, 206, 215, 341
Bse1I ACTGG 1 cut(s) 239
BseBI CCWGG 3 cut(s) 101, 365, 739
BseDI CCNNGG 8 cut(s) 85, 313, 450, 624, 673, 674, 675, 785
BseGI GGATG 1 cut(s) 261
BseLI CCNNNNNNNGG 3 cut(s) 17, 657, 679
BseNI ACTGG 1 cut(s) 239
BseRI GAGGAG 4 cut(s) 74, 416, 773, 802
BseX3I CGGCCG 2 cut(s) 133, 217
BseXI GCAGC 2 cut(s) 49, 332
BseYI CCCAGC 1 cut(s) 728
Bsh1236I CGCG 5 cut(s) 150, 296, 309, 315, 535
Bsh1285I CGRYCG 3 cut(s) 136, 220, 313
BshFI GGCC 6 cut(s) 14, 81, 104, 135, 219, 318
BshNI GGYRCC 2 cut(s) 20, 281
BsiEI CGRYCG 3 cut(s) 136, 220, 313
BsiHKAI GWGCWC 1 cut(s) 612
BsiHKCI CYCGRG 2 cut(s) 140, 674
BsiSI CCGG 9 cut(s) 11, 66, 132, 207, 216, 342, 384, 651, 675
BslFI GGGAC 1 cut(s) 229
BslI CCNNNNNNNGG 3 cut(s) 17, 657, 679
BsmAI GTCTC 2 cut(s) 251, 861
BsmFI GGGAC 1 cut(s) 229
BsmI GAATGC 1 cut(s) 510
BsnI GGCC 6 cut(s) 14, 81, 104, 135, 219, 318
Bso31I GGTCTC 1 cut(s) 251
BsoBI CYCGRG 2 cut(s) 140, 674
Bsp1286I GDGCHC 1 cut(s) 612
Bsp1407I TGTACA 1 cut(s) 708
Bsp143I GATC 4 cut(s) 51, 425, 460, 696
Bsp19I CCATGG 1 cut(s) 450
BspANI GGCC 6 cut(s) 14, 81, 104, 135, 219, 318
BspFNI CGCG 5 cut(s) 150, 296, 309, 315, 535
BspLI GGNNCC 4 cut(s) 22, 130, 283, 382
BspPI GGATC 2 cut(s) 433, 468
BspT107I GGYRCC 2 cut(s) 20, 281
BspTNI GGTCTC 1 cut(s) 251
BsrFI RCCGGY 4 cut(s) 65, 206, 215, 341
BsrGI TGTACA 1 cut(s) 708
BsrI ACTGG 1 cut(s) 239
BssAI RCCGGY 4 cut(s) 65, 206, 215, 341
BssECI CCNNGG 8 cut(s) 85, 313, 450, 624, 673, 674, 675, 785
BssMI GATC 4 cut(s) 51, 425, 460, 696
BssNI GRCGYC 1 cut(s) 21
BssT1I CCWWGG 1 cut(s) 450
Bst2UI CCWGG 3 cut(s) 101, 365, 739
BstACI GRCGYC 1 cut(s) 21
BstAPI GCANNNNNTGC 1 cut(s) 96
BstAUI TGTACA 1 cut(s) 708
BstC8I GCNNGC 2 cut(s) 208, 765
BstDEI CTNAG 1 cut(s) 897
BstDSI CCRYGG 3 cut(s) 313, 450, 624
BstF5I GGATG 1 cut(s) 261
BstFNI CGCG 5 cut(s) 150, 296, 309, 315, 535
BstH2I RGCGCY 1 cut(s) 24
BstHHI GCGC 2 cut(s) 23, 152
BstKTI GATC 4 cut(s) 54, 428, 463, 699
BstMAI GTCTC 2 cut(s) 251, 861
BstMBI GATC 4 cut(s) 51, 425, 460, 696
BstMCI CGRYCG 3 cut(s) 136, 220, 313
BstNI CCWGG 3 cut(s) 101, 365, 739
BstSCI CCNGG 5 cut(s) 99, 363, 673, 674, 737
BstUI CGCG 5 cut(s) 150, 296, 309, 315, 535
BstV1I GCAGC 2 cut(s) 49, 332
BstV2I GAAGAC 1 cut(s) 187
BstX2I RGATCY 3 cut(s) 425, 460, 696
BstXI CCANNNNNNTGG 2 cut(s) 451, 625
BstYI RGATCY 3 cut(s) 425, 460, 696
BstZI CGGCCG 2 cut(s) 133, 217
BsuRI GGCC 6 cut(s) 14, 81, 104, 135, 219, 318
BtgI CCRYGG 3 cut(s) 313, 450, 624
BtgZI GCGATG 1 cut(s) 154
BtrI CACGTC 1 cut(s) 193
BtsCI GGATG 1 cut(s) 261
Cac8I GCNNGC 2 cut(s) 208, 765
CfoI GCGC 2 cut(s) 23, 152
Cfr10I RCCGGY 4 cut(s) 65, 206, 215, 341
Cfr13I GGNCC 2 cut(s) 103, 298
Cfr42I CCGCGG 1 cut(s) 316
Cfr9I CCCGGG 1 cut(s) 674
CseI GACGC 1 cut(s) 798
Csp6I GTAC 4 cut(s) 124, 358, 489, 709
CviAII CATG 2 cut(s) 451, 592
CviQI GTAC 4 cut(s) 124, 358, 489, 709
DdeI CTNAG 1 cut(s) 897
DinI GGCGCC 1 cut(s) 22
DpnI GATC 4 cut(s) 53, 427, 462, 698
DpnII GATC 4 cut(s) 51, 425, 460, 696
EaeI YGGCCR 3 cut(s) 12, 133, 217
EagI CGGCCG 2 cut(s) 133, 217
Ecl136II GAGCTC 1 cut(s) 610
EclXI CGGCCG 2 cut(s) 133, 217
Eco130I CCWWGG 1 cut(s) 450
Eco24I GRGCYC 1 cut(s) 612
Eco31I GGTCTC 1 cut(s) 251
Eco47I GGWCC 1 cut(s) 298
Eco52I CGGCCG 2 cut(s) 133, 217
Eco53kI GAGCTC 1 cut(s) 610
Eco88I CYCGRG 2 cut(s) 140, 674
EcoICRI GAGCTC 1 cut(s) 610
EcoRII CCWGG 3 cut(s) 99, 363, 737
EcoT14I CCWWGG 1 cut(s) 450
EcoT38I GRGCYC 1 cut(s) 612
EgeI GGCGCC 1 cut(s) 22
EheI GGCGCC 1 cut(s) 22
ErhI CCWWGG 1 cut(s) 450
FaeI CATG 2 cut(s) 454, 595
FaiI YATR 4 cut(s) 97, 452, 564, 593
FaqI GGGAC 1 cut(s) 229
FatI CATG 2 cut(s) 450, 591
FauI CCCGC 1 cut(s) 540
FblI GTMKAC 1 cut(s) 231
Fnu4HI GCNGC 9 cut(s) 38, 79, 136, 174, 204, 220, 307, 316, 346
FokI GGATG 1 cut(s) 248
FriOI GRGCYC 1 cut(s) 612
Fsp4HI GCNGC 9 cut(s) 38, 79, 136, 174, 204, 220, 307, 316, 346
GlaI GCGC 2 cut(s) 22, 151
GluI GCNGC 9 cut(s) 38, 79, 136, 174, 204, 220, 307, 316, 346
GsaI CCCAGC 1 cut(s) 732
HaeII RGCGCY 1 cut(s) 24
HaeIII GGCC 6 cut(s) 14, 81, 104, 135, 219, 318
HapII CCGG 9 cut(s) 11, 66, 132, 207, 216, 342, 384, 651, 675
HgaI GACGC 1 cut(s) 798
HhaI GCGC 2 cut(s) 23, 152
Hin1I GRCGYC 1 cut(s) 21
Hin1II CATG 2 cut(s) 454, 595
Hin6I GCGC 2 cut(s) 21, 150
HinP1I GCGC 2 cut(s) 21, 150
HincII GTYRAC 3 cut(s) 232, 331, 577
HindII GTYRAC 3 cut(s) 232, 331, 577
HinfI GANTC 4 cut(s) 242, 643, 654, 854
HpaII CCGG 9 cut(s) 11, 66, 132, 207, 216, 342, 384, 651, 675
HphI GGTGA 5 cut(s) 362, 538, 640, 727, 784
Hpy166II GTNNAC 3 cut(s) 232, 331, 577
Hpy188I TCNGA 3 cut(s) 425, 520, 786
Hpy188III TCNNGA 7 cut(s) 49, 142, 256, 469, 658, 755, 865
Hpy8I GTNNAC 3 cut(s) 232, 331, 577
Hpy99I CGWCG 3 cut(s) 233, 278, 281
HpyAV CCTTC 2 cut(s) 371, 623
HpyCH4IV ACGT 3 cut(s) 192, 228, 273
HpyCH4V TGCA 2 cut(s) 348, 526
HpyF3I CTNAG 1 cut(s) 897
HpySE526I ACGT 3 cut(s) 192, 228, 273
Hsp92I GRCGYC 1 cut(s) 21
Hsp92II CATG 2 cut(s) 454, 595
HspAI GCGC 2 cut(s) 21, 150
KasI GGCGCC 1 cut(s) 20
KroI GCCGGC 1 cut(s) 206
KroNI GCCGGC 1 cut(s) 208
KspI CCGCGG 1 cut(s) 316
Kzo9I GATC 4 cut(s) 51, 425, 460, 696
LmnI GCTCC 6 cut(s) 134, 386, 615, 760, 787, 789
Lsp1109I GCAGC 2 cut(s) 49, 332
LweI GCATC 2 cut(s) 396, 796
MaeII ACGT 3 cut(s) 192, 228, 273
MaeIII GTNAC 1 cut(s) 211
MalI GATC 4 cut(s) 53, 427, 462, 698
MboI GATC 4 cut(s) 51, 425, 460, 696
MboII GAAGA 3 cut(s) 187, 754, 832
MflI RGATCY 3 cut(s) 425, 460, 696
MhlI GDGCHC 1 cut(s) 612
MluCI AATT 2 cut(s) 472, 815
Mly113I GGCGCC 1 cut(s) 21
MlyI GAGTC 2 cut(s) 251, 863
MreI CGCCGGCG 1 cut(s) 206
MroNI GCCGGC 1 cut(s) 206
MroXI GAANNNNTTC 3 cut(s) 746, 827, 872
MslI CAYNNNNRTG 1 cut(s) 623
MspA1I CMGCKG 1 cut(s) 315
MspI CCGG 9 cut(s) 11, 66, 132, 207, 216, 342, 384, 651, 675
MspR9I CCNGG 5 cut(s) 101, 365, 675, 676, 739
Mva1269I GAATGC 1 cut(s) 510
MvaI CCWGG 3 cut(s) 101, 365, 739
MvnI CGCG 5 cut(s) 150, 296, 309, 315, 535
NaeI GCCGGC 1 cut(s) 208
NarI GGCGCC 1 cut(s) 21
NciI CCSGG 2 cut(s) 675, 676
NcoI CCATGG 1 cut(s) 450
NdeII GATC 4 cut(s) 51, 425, 460, 696
NgoMIV GCCGGC 1 cut(s) 206
NlaIII CATG 2 cut(s) 454, 595
NlaIV GGNNCC 4 cut(s) 22, 130, 283, 382
NmeAIII GCCGAG 1 cut(s) 66
NmuCI GTSAC 1 cut(s) 211
PaeR7I CTCGAG 1 cut(s) 140
PcsI WCGNNNNNNNCGW 2 cut(s) 207, 237
PctI GAATGC 1 cut(s) 510
PdiI GCCGGC 1 cut(s) 208
PdmI GAANNNNTTC 3 cut(s) 746, 827, 872
PfeI GAWTC 2 cut(s) 643, 654
PkrI GCNGC 9 cut(s) 39, 80, 137, 175, 205, 221, 308, 317, 347
PleI GAGTC 2 cut(s) 250, 862
PluTI GGCGCC 1 cut(s) 24
PpsI GAGTC 2 cut(s) 250, 862
Psp124BI GAGCTC 1 cut(s) 612
Psp6I CCWGG 3 cut(s) 99, 363, 737
PspFI CCCAGC 1 cut(s) 728
PspGI CCWGG 3 cut(s) 99, 363, 737
PspN4I GGNNCC 4 cut(s) 22, 130, 283, 382
PspPI GGNCC 2 cut(s) 103, 298
PsuI RGATCY 3 cut(s) 425, 460, 696
RsaI GTAC 4 cut(s) 125, 359, 490, 710
RsaNI GTAC 4 cut(s) 124, 358, 489, 709
RseI CAYNNNNRTG 1 cut(s) 623
SacI GAGCTC 1 cut(s) 612
SacII CCGCGG 1 cut(s) 316
SalI GTCGAC 1 cut(s) 230
SatI GCNGC 9 cut(s) 38, 79, 136, 174, 204, 220, 307, 316, 346
Sau3AI GATC 4 cut(s) 51, 425, 460, 696
Sau96I GGNCC 2 cut(s) 103, 298
SchI GAGTC 2 cut(s) 251, 863
ScrFI CCNGG 5 cut(s) 101, 365, 675, 676, 739
SduI GDGCHC 1 cut(s) 612
SfaNI GCATC 2 cut(s) 396, 796
SfoI GGCGCC 1 cut(s) 22
Sfr274I CTCGAG 1 cut(s) 140
Sfr303I CCGCGG 1 cut(s) 316
SgrAI CRCCGGYG 1 cut(s) 206
SgrBI CCGCGG 1 cut(s) 316
SinI GGWCC 1 cut(s) 298
SlaI CTCGAG 1 cut(s) 140
SmaI CCCGGG 1 cut(s) 676
SmiMI CAYNNNNRTG 1 cut(s) 623
SmlI CTYRAG 4 cut(s) 41, 140, 467, 755
SmoI CTYRAG 4 cut(s) 41, 140, 467, 755
Sse9I AATT 2 cut(s) 472, 815
SspDI GGCGCC 1 cut(s) 20
SstI GAGCTC 1 cut(s) 612
StyD4I CCNGG 5 cut(s) 99, 363, 673, 674, 737
StyI CCWWGG 1 cut(s) 450
TaiI ACGT 3 cut(s) 195, 231, 276
TaqI TCGA 7 cut(s) 120, 141, 159, 231, 255, 354, 723
TasI AATT 2 cut(s) 472, 815
TatI WGTACW 2 cut(s) 488, 708
TauI GCSGC 7 cut(s) 81, 138, 176, 206, 222, 309, 318
TfiI GAWTC 2 cut(s) 643, 654
TseFI GTSAC 1 cut(s) 211
TseI GCWGC 2 cut(s) 37, 345
Tsp45I GTSAC 1 cut(s) 211
TspDTI ATGAA 2 cut(s) 504, 532
TspGWI ACGGA 2 cut(s) 485, 613
TspMI CCCGGG 1 cut(s) 674
VpaK11BI GGWCC 1 cut(s) 298
XcmI CCANNNNNNNNNTGG 2 cut(s) 451, 598
XhoI CTCGAG 1 cut(s) 140
XmaI CCCGGG 1 cut(s) 674
XmiI GTMKAC 1 cut(s) 231
XmnI GAANNNNTTC 3 cut(s) 746, 827, 872
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.