Rh1CG041300

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0002404
Physical Location & Seq
Reverse (-)
7746856 .. 7754360
7505 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG041300.1

Sequence Viewer

Length: 1533 bp
ATGAGCGCTGATCCTGACTACCTCAGTTGCAAGAAGAAGGAATGCAAAAAGTATCAGAATGGTACCAGTCACTGTGCTATCTCCACCTGCAGTGGTTCGTTAACATTTCATGTTATTAACATCAGAACCGACATCGAATTCGTCTTGTTTTCTGGTGGATTTGAGGTCCCTTGCATTTTGAAGAAAGCAAACCCTCTGAAATTTGCCAATCCAAAGAAGCCACTGTATGGACATCTCTCGAGCATAGACTCAACTGGAACATCGATGAAATTAACATGGGTCAGTGGAGATGAGAAACCTCAGCAAGTACAATATGGAGATGGAAAATCACAGACTTCAGTAGTTACCACATTTTCACAAGATGACATGCAGAGTTTACCTGCTATACCAAGTCCAGCCAAAGACTTTGGGTGGCATGATCCAGGCTTCATTCATTCAGCAGTGATGACAGGACTTGAGCCTTCATCTAGCTTCTCTTATAGATATGGAAGTGATTCAGTTGGTTGGAGTGATCGAATCCAATTTCGCACTCCCCCTGTGGGAGGATCTGATGAACTCAAATTTCTTGCATTTGGCGATATGGGAAAGGCTCCTCGTGATTCTTCTGTAGAGCATTATATTCAGCCAGGATCCCTCTCAGTGATTGAAGCCGTGACAGATGAAATAAATTCCGGGAATGTAGACTCTGTCTTCCACATTGGAGATATAAGCTATGCCACTGGGTTTTTAGTTGAATGGGATTTTTTCCTTCACCAAATCAACCCTGTTGCTTCTCGAGTTTCATACATGACAGCTATCGGGAACCATGAGAGGGACTATATAGACTCTGGATCAGTGTACGTTCTTGCAGATTCAGGTGGGGAAGTTGGAGTTCCCTATGAGACTTACTTTCCAATGCCAACCCCAGCAAAGGATAAACCATGGTATTCCATAGAACAAGGAAGCGTCCACATCACGATGATTTCAACAGAGCATGACTGGACCAAAAATTCTGAGCAGTATCAATGGATGAAAAAGGACATGGCTTCAGTTGATCGATCTAAAACTCGTTGGTTGATATTTATGGGGCATAGACCAATGTATACATCTGCCCCTGGATTCCTGAGTGTTGATGAAAAATTTGTTGACGAAGTTGAACCATTACTTCTGGCAAGCAAAGTTGATCTGGCCCTGTTTGGTCATGTCCATAACTACGAGCGAACCTGCTCCATTTATAAGAGCCAGTGCATGAAATTGCCTAGCAAAGATGAGGCTGGGATAGACACATATGATCACAGCAACTACAGTGCCCCAGTGCAAGCTATAATTGGAATGGCTGGCTTTTCCTTGGACAAGTTTCCCAGTGGTGTAAGTCCTTTACTCTGTCATATCTCTCTTCAAAAATGTGCACTTAATTGTACAAGCATGTTCTTCATTGTTCTTTACATGGAATTGGGACTTCATTCAAACAGATTAACAATAGTTGGAGTTTATCAAGGCATTCCGAATATGGTTATCTCAGAGCGCATGCAACAAGGACAGATATCAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003993 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0044237
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

510

Amino Acids

56.6

Weight (kDa)

5.63

Isoelectric Point (pI)

39.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
fn3_PAP PF17808 18 - 70 6.8e-14 Fn3-like domain from Purple Acid Phosphatase
Pur_ac_phosph_N PF16656 76 - 177 6.2e-16 Purple acid Phosphatase, N-terminal domain
Metallophos PF00149 186 - 398 2.1e-16 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1215
AarI CACCTGC 1 cut(s) 95
Acc36I ACCTGC 3 cut(s) 95, 388, 1211
Acc65I GGTACC 1 cut(s) 62
AccB1I GGYRCC 1 cut(s) 62
AccB7I CCANNNNNTGG 1 cut(s) 227
AccI GTMKAC 2 cut(s) 681, 1082
AclWI GGATC 6 cut(s) 5, 413, 553, 624, 637, 838
AcsI RAATTY 6 cut(s) 137, 200, 560, 667, 988, 1118
AcuI CTGAAG 2 cut(s) 321, 1011
AfaI GTAC 4 cut(s) 64, 309, 839, 1399
AfeI AGCGCT 1 cut(s) 7
AfiI CCNNNNNNNGG 5 cut(s) 227, 539, 542, 811, 910
AgsI TTSAA 7 cut(s) 181, 647, 734, 966, 1136, 1379, 1446
AjnI CCWGG 3 cut(s) 421, 625, 1093
AluBI AGCT 4 cut(s) 471, 711, 794, 1301
AluI AGCT 4 cut(s) 471, 711, 794, 1301
Alw21I GWGCWC 1 cut(s) 1390
Alw26I GTCTC 1 cut(s) 875
Alw44I GTGCAC 1 cut(s) 1386
AlwI GGATC 6 cut(s) 5, 413, 553, 624, 637, 838
AlwNI CAGNNNCTG 1 cut(s) 72
Ama87I CYCGRG 2 cut(s) 238, 774
Aor51HI AGCGCT 1 cut(s) 7
AoxI GGCC 1 cut(s) 1167
ApaLI GTGCAC 1 cut(s) 1386
ApoI RAATTY 6 cut(s) 137, 200, 560, 667, 988, 1118
ArsI GACNNNNNNTTYG 2 cut(s) 122, 154
Asp700I GAANNNNTTC 1 cut(s) 493
Asp718I GGTACC 1 cut(s) 62
AspLEI GCGC 2 cut(s) 8, 1506
AspS9I GGNCC 3 cut(s) 166, 981, 1168
AsuC2I CCSGG 1 cut(s) 673
AsuHPI GGTGA 1 cut(s) 743
AvaI CYCGRG 2 cut(s) 238, 774
AvaII GGWCC 2 cut(s) 166, 981
BaeGI GKGCMC 2 cut(s) 1291, 1390
BamHI GGATCC 1 cut(s) 629
BanI GGYRCC 1 cut(s) 62
BauI CACGAG 1 cut(s) 594
BbsI GAAGAC 1 cut(s) 682
Bbv12I GWGCWC 1 cut(s) 1390
BbvCI CCTCAGC 1 cut(s) 300
BccI CCATC 1 cut(s) 314
BceAI ACGGC 1 cut(s) 635
BciT130I CCWGG 3 cut(s) 423, 627, 1095
BclI TGATCA 1 cut(s) 1270
BcnI CCSGG 1 cut(s) 673
BcoDI GTCTC 1 cut(s) 875
BfaI CTAG 2 cut(s) 468, 1239
BfmI CTRYAG 3 cut(s) 88, 606, 1282
BfoI RGCGCY 1 cut(s) 9
BfuAI ACCTGC 3 cut(s) 95, 388, 1211
Bme1390I CCNGG 4 cut(s) 423, 627, 673, 1095
Bme18I GGWCC 2 cut(s) 166, 981
BmeT110I CYCGRG 2 cut(s) 238, 774
BmgT120I GGNCC 3 cut(s) 166, 981, 1168
BmiI GGNNCC 5 cut(s) 64, 168, 591, 631, 803
BmrFI CCNGG 4 cut(s) 423, 627, 673, 1095
BmrI ACTGGG 3 cut(s) 729, 1286, 1335
BmuI ACTGGG 3 cut(s) 729, 1286, 1335
BpiI GAAGAC 1 cut(s) 682
Bpu10I CCTNAGC 1 cut(s) 300
BpuEI CTTGAG 1 cut(s) 476
BpuMI CCSGG 1 cut(s) 673
Bsa29I ATCGAT 2 cut(s) 263, 1036
BsaJI CCNNGG 3 cut(s) 920, 1093, 1326
Bsc4I CCNNNNNNNGG 5 cut(s) 227, 539, 542, 811, 910
Bse1I ACTGG 7 cut(s) 66, 259, 724, 983, 1222, 1292, 1341
BseBI CCWGG 3 cut(s) 423, 627, 1095
BseCI ATCGAT 2 cut(s) 263, 1036
BseDI CCNNGG 3 cut(s) 920, 1093, 1326
BseGI GGATG 1 cut(s) 1014
BseLI CCNNNNNNNGG 5 cut(s) 227, 539, 542, 811, 910
BseMII CTCAG 6 cut(s) 37, 314, 651, 984, 1094, 1512
BseNI ACTGG 7 cut(s) 66, 259, 724, 983, 1222, 1292, 1341
BseRI GAGGAG 1 cut(s) 582
BseSI GKGCMC 2 cut(s) 1291, 1390
BseYI CCCAGC 2 cut(s) 904, 1253
BshFI GGCC 1 cut(s) 1169
BshNI GGYRCC 1 cut(s) 62
BshVI ATCGAT 2 cut(s) 263, 1036
BsiHKAI GWGCWC 1 cut(s) 1390
BsiHKCI CYCGRG 2 cut(s) 238, 774
BsiSI CCGG 1 cut(s) 672
BslFI GGGAC 3 cut(s) 152, 827, 1449
BslI CCNNNNNNNGG 5 cut(s) 227, 539, 542, 811, 910
BsmAI GTCTC 1 cut(s) 875
BsmFI GGGAC 3 cut(s) 152, 827, 1449
BsmI GAATGC 2 cut(s) 47, 1479
BsnI GGCC 1 cut(s) 1169
BsoBI CYCGRG 2 cut(s) 238, 774
Bsp1286I GDGCHC 2 cut(s) 1291, 1390
Bsp1407I TGTACA 1 cut(s) 1397
Bsp19I CCATGG 1 cut(s) 920
BspANI GGCC 1 cut(s) 1169
BspCNI CTCAG 6 cut(s) 36, 313, 650, 985, 1095, 1511
BspDI ATCGAT 2 cut(s) 263, 1036
BspLI GGNNCC 5 cut(s) 64, 168, 591, 631, 803
BspMAI CTGCAG 1 cut(s) 92
BspMI ACCTGC 3 cut(s) 95, 388, 1211
BspPI GGATC 6 cut(s) 5, 413, 553, 624, 637, 838
BspT107I GGYRCC 1 cut(s) 62
BsrGI TGTACA 1 cut(s) 1397
BsrI ACTGG 7 cut(s) 66, 259, 724, 983, 1222, 1292, 1341
BssECI CCNNGG 3 cut(s) 920, 1093, 1326
BssNAI GTATAC 1 cut(s) 1083
BssSI CACGAG 1 cut(s) 594
BssT1I CCWWGG 2 cut(s) 920, 1326
Bst1107I GTATAC 1 cut(s) 1083
Bst2BI CACGAG 1 cut(s) 594
Bst2UI CCWGG 3 cut(s) 423, 627, 1095
Bst4CI ACNGT 3 cut(s) 74, 225, 1286
Bst6I CTCTTC 1 cut(s) 1380
BstAUI TGTACA 1 cut(s) 1397
BstC8I GCNNGC 4 cut(s) 1153, 1299, 1318, 1508
BstDEI CTNAG 6 cut(s) 23, 300, 637, 993, 1103, 1498
BstDSI CCRYGG 1 cut(s) 920
BstENI CCTNNNNNAGG 1 cut(s) 540
BstF5I GGATG 1 cut(s) 1014
BstH2I RGCGCY 1 cut(s) 9
BstHHI GCGC 2 cut(s) 8, 1506
BstMAI GTCTC 1 cut(s) 875
BstNI CCWGG 3 cut(s) 423, 627, 1095
BstNSI RCATGY 3 cut(s) 370, 1408, 1510
BstSCI CCNGG 4 cut(s) 421, 625, 671, 1093
BstSFI CTRYAG 3 cut(s) 88, 606, 1282
BstSLI GKGCMC 2 cut(s) 1291, 1390
BstV2I GAAGAC 1 cut(s) 682
BstX2I RGATCY 2 cut(s) 545, 629
BstYI RGATCY 2 cut(s) 545, 629
BstZ17I GTATAC 1 cut(s) 1083
Bsu15I ATCGAT 2 cut(s) 263, 1036
BsuRI GGCC 1 cut(s) 1169
BsuTUI ATCGAT 2 cut(s) 263, 1036
BtgI CCRYGG 1 cut(s) 920
BtsCI GGATG 1 cut(s) 1014
BtsI GCAGTG 2 cut(s) 97, 447
BveI ACCTGC 3 cut(s) 95, 388, 1211
Cac8I GCNNGC 4 cut(s) 1153, 1299, 1318, 1508
CaiI CAGNNNCTG 1 cut(s) 72
CfoI GCGC 2 cut(s) 8, 1506
Cfr13I GGNCC 3 cut(s) 166, 981, 1168
ClaI ATCGAT 2 cut(s) 263, 1036
CseI GACGC 1 cut(s) 934
Csp6I GTAC 4 cut(s) 63, 308, 838, 1398
CviQI GTAC 4 cut(s) 63, 308, 838, 1398
DdeI CTNAG 6 cut(s) 23, 300, 637, 993, 1103, 1498
Eam1104I CTCTTC 1 cut(s) 1380
EarI CTCTTC 1 cut(s) 1380
Eco130I CCWWGG 2 cut(s) 920, 1326
Eco32I GATATC 1 cut(s) 1524
Eco47I GGWCC 2 cut(s) 166, 981
Eco47III AGCGCT 1 cut(s) 7
Eco57I CTGAAG 2 cut(s) 321, 1011
Eco88I CYCGRG 2 cut(s) 238, 774
EcoNI CCTNNNNNAGG 1 cut(s) 540
EcoO109I RGGNCCY 1 cut(s) 166
EcoRI GAATTC 1 cut(s) 137
EcoRII CCWGG 3 cut(s) 421, 625, 1093
EcoRV GATATC 1 cut(s) 1524
EcoT14I CCWWGG 2 cut(s) 920, 1326
ErhI CCWWGG 2 cut(s) 920, 1326
FaqI GGGAC 3 cut(s) 152, 827, 1449
FauNDI CATATG 1 cut(s) 1267
FbaI TGATCA 1 cut(s) 1270
FblI GTMKAC 2 cut(s) 681, 1082
FokI GGATG 1 cut(s) 1021
FspBI CTAG 2 cut(s) 468, 1239
GlaI GCGC 2 cut(s) 7, 1505
GsaI CCCAGC 2 cut(s) 908, 1257
HaeII RGCGCY 1 cut(s) 9
HaeIII GGCC 1 cut(s) 1169
HapII CCGG 1 cut(s) 672
HgaI GACGC 1 cut(s) 934
HhaI GCGC 2 cut(s) 8, 1506
Hin6I GCGC 2 cut(s) 6, 1504
HinP1I GCGC 2 cut(s) 6, 1504
HincII GTYRAC 2 cut(s) 102, 1126
HindII GTYRAC 2 cut(s) 102, 1126
HinfI GANTC 8 cut(s) 248, 494, 516, 599, 683, 824, 851, 1098
HpaI GTTAAC 1 cut(s) 102
HpaII CCGG 1 cut(s) 672
HphI GGTGA 1 cut(s) 743
Hpy166II GTNNAC 8 cut(s) 102, 377, 682, 838, 949, 1083, 1126, 1388
Hpy188I TCNGA 7 cut(s) 57, 125, 198, 550, 994, 1485, 1501
Hpy188III TCNNGA 8 cut(s) 14, 238, 596, 774, 799, 828, 955, 1102
Hpy8I GTNNAC 8 cut(s) 102, 377, 682, 838, 949, 1083, 1126, 1388
HpyAV CCTTC 3 cut(s) 31, 471, 758
HpyCH4III ACNGT 3 cut(s) 74, 225, 1286
HpyCH4IV ACGT 1 cut(s) 840
HpyF3I CTNAG 6 cut(s) 23, 300, 637, 993, 1103, 1498
HpySE526I ACGT 1 cut(s) 840
HspAI GCGC 2 cut(s) 6, 1504
KpnI GGTACC 1 cut(s) 66
Ksp22I TGATCA 1 cut(s) 1270
KspAI GTTAAC 1 cut(s) 102
LmnI GCTCC 2 cut(s) 595, 1211
MaeI CTAG 2 cut(s) 468, 1239
MaeII ACGT 1 cut(s) 840
MaeIII GTNAC 3 cut(s) 68, 343, 652
MboII GAAGA 6 cut(s) 46, 193, 594, 682, 1367, 1402
MflI RGATCY 2 cut(s) 545, 629
MhlI GDGCHC 2 cut(s) 1291, 1390
MlyI GAGTC 3 cut(s) 242, 677, 818
MmeI TCCRAC 3 cut(s) 485, 847, 1444
MnlI CCTC 9 cut(s) 32, 157, 204, 309, 536, 603, 644, 804, 1243
MroXI GAANNNNTTC 1 cut(s) 493
MseI TTAA 5 cut(s) 101, 117, 272, 1392, 1454
MslI CAYNNNNRTG 1 cut(s) 956
MspI CCGG 1 cut(s) 672
MspR9I CCNGG 4 cut(s) 423, 627, 673, 1095
Mva1269I GAATGC 2 cut(s) 47, 1479
MvaI CCWGG 3 cut(s) 423, 627, 1095
NciI CCSGG 1 cut(s) 673
NcoI CCATGG 1 cut(s) 920
NdeI CATATG 1 cut(s) 1267
NlaIV GGNNCC 5 cut(s) 64, 168, 591, 631, 803
NmuCI GTSAC 2 cut(s) 68, 652
NspI RCATGY 3 cut(s) 370, 1408, 1510
PaeI GCATGC 1 cut(s) 1510
PaeR7I CTCGAG 2 cut(s) 238, 774
PaqCI CACCTGC 1 cut(s) 95
PctI GAATGC 2 cut(s) 47, 1479
PdmI GAANNNNTTC 1 cut(s) 493
PfeI GAWTC 5 cut(s) 494, 516, 599, 851, 1098
PflFI GACNNNGTC 1 cut(s) 686
PflMI CCANNNNNTGG 1 cut(s) 227
PfoI TCCNGGA 1 cut(s) 671
PleI GAGTC 3 cut(s) 242, 677, 818
PpsI GAGTC 3 cut(s) 242, 677, 818
PpuMI RGGWCCY 1 cut(s) 166
PsiI TTATAA 1 cut(s) 1215
Psp5II RGGWCCY 1 cut(s) 166
Psp6I CCWGG 3 cut(s) 421, 625, 1093
PspFI CCCAGC 2 cut(s) 904, 1253
PspGI CCWGG 3 cut(s) 421, 625, 1093
PspN4I GGNNCC 5 cut(s) 64, 168, 591, 631, 803
PspPI GGNCC 3 cut(s) 166, 981, 1168
PspPPI RGGWCCY 1 cut(s) 166
PstI CTGCAG 1 cut(s) 92
PstNI CAGNNNCTG 1 cut(s) 72
PsuI RGATCY 2 cut(s) 545, 629
PsyI GACNNNGTC 1 cut(s) 686
RsaI GTAC 4 cut(s) 64, 309, 839, 1399
RsaNI GTAC 4 cut(s) 63, 308, 838, 1398
RseI CAYNNNNRTG 1 cut(s) 956
SaqAI TTAA 5 cut(s) 101, 117, 272, 1392, 1454
Sau96I GGNCC 3 cut(s) 166, 981, 1168
SchI GAGTC 3 cut(s) 242, 677, 818
ScrFI CCNGG 4 cut(s) 423, 627, 673, 1095
SduI GDGCHC 2 cut(s) 1291, 1390
SfcI CTRYAG 3 cut(s) 88, 606, 1282
Sfr274I CTCGAG 2 cut(s) 238, 774
SinI GGWCC 2 cut(s) 166, 981
SlaI CTCGAG 2 cut(s) 238, 774
SmiMI CAYNNNNRTG 1 cut(s) 956
SmlI CTYRAG 3 cut(s) 238, 455, 774
SmoI CTYRAG 3 cut(s) 238, 455, 774
SphI GCATGC 1 cut(s) 1510
SspMI CTAG 2 cut(s) 468, 1239
StyD4I CCNGG 4 cut(s) 421, 625, 671, 1093
StyI CCWWGG 2 cut(s) 920, 1326
TaaI ACNGT 3 cut(s) 74, 225, 1286
TaiI ACGT 1 cut(s) 843
TaqI TCGA 6 cut(s) 135, 239, 263, 514, 775, 1036
TatI WGTACW 2 cut(s) 307, 1397
TfiI GAWTC 5 cut(s) 494, 516, 599, 851, 1098
Tru1I TTAA 5 cut(s) 101, 117, 272, 1392, 1454
Tru9I TTAA 5 cut(s) 101, 117, 272, 1392, 1454
TseFI GTSAC 2 cut(s) 68, 652
Tsp45I GTSAC 2 cut(s) 68, 652
Tth111I GACNNNGTC 1 cut(s) 686
Van91I CCANNNNNTGG 1 cut(s) 227
VneI GTGCAC 1 cut(s) 1386
VpaK11BI GGWCC 2 cut(s) 166, 981
XagI CCTNNNNNAGG 1 cut(s) 540
XapI RAATTY 6 cut(s) 137, 200, 560, 667, 988, 1118
XceI RCATGY 3 cut(s) 370, 1408, 1510
XhoI CTCGAG 2 cut(s) 238, 774
XmiI GTMKAC 2 cut(s) 681, 1082
XmnI GAANNNNTTC 1 cut(s) 493
XspI CTAG 2 cut(s) 468, 1239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.