MD11G1013000.v1.1

DNA (cytosine-5)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0001701
Physical Location & Seq
Reverse (-)
971607 .. 973029
1423 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1013000.v1.1.491

Sequence Viewer

Length: 651 bp
ATGTGTTCACATGTTATTTCTGGTTTTGAAATTTCAGAAAATTGCAGTCTGGAAGTGATGACTCAACTATCTCTGTTTCAGATGATGTTTTGTTTGGATAGTGAGGTTGATTCAAAGATGTCAAATGGAGTGTGTGCGAAATCAGAAGTAAGCATCCTGGACCTATATTTGGGTTGTGAAGCGATGTCCACTGGCTTGTGCCTTGGTGCACGTTTGGGCAGTGTCAATCTTGTAACAGTCCAAAATGAAGCTGCTGAAGATTTCCTCAAAATGCTCAAGGAATGGAGAAAATACTGCTTCTGCTTAAAGTTGGTTGAGACTGACAATTTAGACGCAGATGTCGAGAAGAGTATGCTTGAATTCTTTAGGAAGGAAGATGATGAAGACGAAGAAGATGTTAAAGGAAATGTTAAGGATGATTCTGAAATGTTTGAAGTTGACTACGTAGGAGTATGTTTTGGTGACTCCAAAAAAAATGAGAAGAAGGGAATATACTTAAAGATTTATTGGAAGGGCTACAGGCCTGAGGAAGACACCTGGGAGCCAATGAATGAATTGGAGTCTGCAACTATTCTTATTTATGCTTGCAGCATTATCTGTTGCATGTTTTGTATCATTTATGTAAAAGGCTATCGAAGACTTAATTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko01100 ko05206 map00270 map01100 map05206
Pfam Domains
Protein Families
BAH Chromo DNA_methylase

Protein Analysis

217

Amino Acids

24.81

Weight (kDa)

4.48

Isoelectric Point (pI)

32.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chromo PF00385 155 - 189 8.7e-06 Chromo (CHRromatin Organisation MOdifier) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 338
AcsI RAATTY 2 cut(s) 30, 359
AcuI CTGAAG 1 cut(s) 276
AfiI CCNNNNNNNGG 1 cut(s) 169
AflIII ACRYGT 1 cut(s) 10
AgsI TTSAA 4 cut(s) 29, 114, 359, 434
AjnI CCWGG 2 cut(s) 156, 536
AluBI AGCT 1 cut(s) 251
AluI AGCT 1 cut(s) 251
Alw21I GWGCWC 1 cut(s) 211
Alw26I GTCTC 1 cut(s) 311
Alw44I GTGCAC 1 cut(s) 207
AoxI GGCC 1 cut(s) 521
ApaLI GTGCAC 1 cut(s) 207
ApeKI GCWGC 2 cut(s) 251, 588
ApoI RAATTY 2 cut(s) 30, 359
AspS9I GGNCC 1 cut(s) 160
AsuHPI GGTGA 1 cut(s) 473
AvaII GGWCC 1 cut(s) 160
AxyI CCTNAGG 1 cut(s) 525
BaeGI GKGCMC 1 cut(s) 211
BarI GAAGNNNNNNTAC 2 cut(s) 476, 508
BbsI GAAGAC 3 cut(s) 390, 537, 643
Bbv12I GWGCWC 1 cut(s) 211
BbvI GCAGC 2 cut(s) 238, 600
BciT130I CCWGG 2 cut(s) 158, 538
BcoDI GTCTC 1 cut(s) 311
BfmI CTRYAG 1 cut(s) 517
BisI GCNGC 2 cut(s) 252, 589
BlsI GCNGC 2 cut(s) 253, 590
Bme1390I CCNGG 2 cut(s) 158, 538
Bme18I GGWCC 1 cut(s) 160
BmgT120I GGNCC 1 cut(s) 160
BmiI GGNNCC 1 cut(s) 543
BmrFI CCNGG 2 cut(s) 158, 538
BmsI GCATC 1 cut(s) 162
BpiI GAAGAC 3 cut(s) 390, 537, 643
BpuEI CTTGAG 1 cut(s) 260
BsaAI YACGTR 1 cut(s) 445
BsaJI CCNNGG 2 cut(s) 202, 537
Bsc4I CCNNNNNNNGG 1 cut(s) 169
Bse1I ACTGG 1 cut(s) 196
Bse21I CCTNAGG 1 cut(s) 525
BseBI CCWGG 2 cut(s) 158, 538
BseDI CCNNGG 2 cut(s) 202, 537
BseGI GGATG 2 cut(s) 153, 421
BseLI CCNNNNNNNGG 1 cut(s) 169
BseMII CTCAG 1 cut(s) 516
BseNI ACTGG 1 cut(s) 196
BseSI GKGCMC 1 cut(s) 211
BseXI GCAGC 2 cut(s) 238, 600
BshFI GGCC 1 cut(s) 523
BsiHKAI GWGCWC 1 cut(s) 211
BslI CCNNNNNNNGG 1 cut(s) 169
BsmAI GTCTC 1 cut(s) 311
BsnI GGCC 1 cut(s) 523
Bsp1286I GDGCHC 1 cut(s) 211
BspANI GGCC 1 cut(s) 523
BspCNI CTCAG 1 cut(s) 517
BspLI GGNNCC 1 cut(s) 543
BsrI ACTGG 1 cut(s) 196
BssECI CCNNGG 2 cut(s) 202, 537
BssT1I CCWWGG 1 cut(s) 202
Bst2UI CCWGG 2 cut(s) 158, 538
Bst4CI ACNGT 1 cut(s) 238
Bst6I CTCTTC 1 cut(s) 341
BstBAI YACGTR 1 cut(s) 445
BstC8I GCNNGC 1 cut(s) 586
BstDEI CTNAG 1 cut(s) 525
BstF5I GGATG 2 cut(s) 153, 421
BstMAI GTCTC 1 cut(s) 311
BstNI CCWGG 2 cut(s) 158, 538
BstNSI RCATGY 2 cut(s) 14, 607
BstSCI CCNGG 2 cut(s) 156, 536
BstSFI CTRYAG 1 cut(s) 517
BstSLI GKGCMC 1 cut(s) 211
BstSNI TACGTA 1 cut(s) 445
BstV1I GCAGC 2 cut(s) 238, 600
BstV2I GAAGAC 3 cut(s) 390, 537, 643
Bsu36I CCTNAGG 1 cut(s) 525
BsuRI GGCC 1 cut(s) 523
BtgZI GCGATG 1 cut(s) 197
BtsCI GGATG 2 cut(s) 153, 421
BtsI GCAGTG 1 cut(s) 226
BtsIMutI CAGTG 2 cut(s) 189, 226
Cac8I GCNNGC 1 cut(s) 586
Cfr13I GGNCC 1 cut(s) 160
CseI GACGC 1 cut(s) 341
CviAII CATG 2 cut(s) 11, 604
CviJI RGCY 6 cut(s) 195, 251, 516, 523, 544, 630
CviKI_1 RGCY 6 cut(s) 195, 251, 516, 523, 544, 630
DdeI CTNAG 1 cut(s) 525
DrdI GACNNNNNNGTC 1 cut(s) 338
DseDI GACNNNNNNGTC 1 cut(s) 338
Eam1104I CTCTTC 1 cut(s) 341
EarI CTCTTC 1 cut(s) 341
Eco105I TACGTA 1 cut(s) 445
Eco130I CCWWGG 1 cut(s) 202
Eco147I AGGCCT 1 cut(s) 523
Eco47I GGWCC 1 cut(s) 160
Eco57I CTGAAG 1 cut(s) 276
Eco81I CCTNAGG 1 cut(s) 525
EcoRI GAATTC 1 cut(s) 359
EcoRII CCWGG 2 cut(s) 156, 536
EcoT14I CCWWGG 1 cut(s) 202
ErhI CCWWGG 1 cut(s) 202
FaeI CATG 2 cut(s) 14, 607
FaiI YATR 8 cut(s) 12, 166, 353, 454, 493, 582, 605, 621
FatI CATG 2 cut(s) 10, 603
Fnu4HI GCNGC 2 cut(s) 252, 589
FokI GGATG 2 cut(s) 140, 428
Fsp4HI GCNGC 2 cut(s) 252, 589
GluI GCNGC 2 cut(s) 252, 589
HaeIII GGCC 1 cut(s) 523
HgaI GACGC 1 cut(s) 341
Hin1II CATG 2 cut(s) 14, 607
HincII GTYRAC 1 cut(s) 439
HindII GTYRAC 1 cut(s) 439
HinfI GANTC 5 cut(s) 61, 110, 419, 464, 560
HphI GGTGA 1 cut(s) 473
Hpy166II GTNNAC 4 cut(s) 8, 189, 209, 439
Hpy188I TCNGA 4 cut(s) 37, 81, 145, 424
Hpy188III TCNNGA 2 cut(s) 50, 343
Hpy8I GTNNAC 4 cut(s) 8, 189, 209, 439
HpyAV CCTTC 3 cut(s) 364, 478, 505
HpyCH4III ACNGT 1 cut(s) 238
HpyCH4IV ACGT 2 cut(s) 211, 444
HpyCH4V TGCA 5 cut(s) 45, 209, 566, 588, 603
HpyF3I CTNAG 1 cut(s) 525
HpySE526I ACGT 2 cut(s) 211, 444
Hsp92II CATG 2 cut(s) 14, 607
LmnI GCTCC 1 cut(s) 541
LpnPI CCDG 9 cut(s) 6, 35, 143, 170, 177, 505, 523, 537, 550
Lsp1109I GCAGC 2 cut(s) 238, 600
LweI GCATC 1 cut(s) 162
MaeII ACGT 2 cut(s) 211, 444
MaeIII GTNAC 2 cut(s) 232, 461
MboII GAAGA 9 cut(s) 269, 358, 386, 395, 401, 404, 493, 542, 648
MhlI GDGCHC 1 cut(s) 211
MluCI AATT 6 cut(s) 30, 40, 325, 359, 554, 643
MlyI GAGTC 3 cut(s) 55, 458, 569
MnlI CCTC 3 cut(s) 97, 275, 520
MseI TTAA 5 cut(s) 305, 399, 411, 497, 642
MspR9I CCNGG 2 cut(s) 158, 538
MvaI CCWGG 2 cut(s) 158, 538
NlaIII CATG 2 cut(s) 14, 607
NlaIV GGNNCC 1 cut(s) 543
NmuCI GTSAC 1 cut(s) 461
NspI RCATGY 2 cut(s) 14, 607
PceI AGGCCT 1 cut(s) 523
PciI ACATGT 1 cut(s) 10
PcsI WCGNNNNNNNCGW 1 cut(s) 339
PfeI GAWTC 2 cut(s) 110, 419
PfoI TCCNGGA 1 cut(s) 156
PkrI GCNGC 2 cut(s) 253, 590
PleI GAGTC 3 cut(s) 55, 458, 568
PpsI GAGTC 3 cut(s) 55, 458, 568
Ppu21I YACGTR 1 cut(s) 445
PscI ACATGT 1 cut(s) 10
Psp6I CCWGG 2 cut(s) 156, 536
PspGI CCWGG 2 cut(s) 156, 536
PspN4I GGNNCC 1 cut(s) 543
PspPI GGNCC 1 cut(s) 160
SaqAI TTAA 5 cut(s) 305, 399, 411, 497, 642
SatI GCNGC 2 cut(s) 252, 589
Sau96I GGNCC 1 cut(s) 160
SchI GAGTC 3 cut(s) 55, 458, 569
ScrFI CCNGG 2 cut(s) 158, 538
SduI GDGCHC 1 cut(s) 211
SetI ASST 6 cut(s) 108, 165, 214, 253, 447, 539
SfaNI GCATC 1 cut(s) 162
SfcI CTRYAG 1 cut(s) 517
SinI GGWCC 1 cut(s) 160
SmlI CTYRAG 1 cut(s) 275
SmoI CTYRAG 1 cut(s) 275
SnaBI TACGTA 1 cut(s) 445
Sse9I AATT 6 cut(s) 30, 40, 325, 359, 554, 643
SseBI AGGCCT 1 cut(s) 523
StuI AGGCCT 1 cut(s) 523
StyD4I CCNGG 2 cut(s) 156, 536
StyI CCWWGG 1 cut(s) 202
TaaI ACNGT 1 cut(s) 238
TaiI ACGT 2 cut(s) 214, 447
TaqI TCGA 2 cut(s) 342, 634
TasI AATT 6 cut(s) 30, 40, 325, 359, 554, 643
TfiI GAWTC 2 cut(s) 110, 419
Tru1I TTAA 5 cut(s) 305, 399, 411, 497, 642
Tru9I TTAA 5 cut(s) 305, 399, 411, 497, 642
TscAI CASTG 2 cut(s) 196, 226
TseFI GTSAC 1 cut(s) 461
TseI GCWGC 2 cut(s) 251, 588
Tsp45I GTSAC 1 cut(s) 461
TspDTI ATGAA 4 cut(s) 261, 396, 563, 567
TspRI CASTG 2 cut(s) 196, 226
VneI GTGCAC 1 cut(s) 207
VpaK11BI GGWCC 1 cut(s) 160
XapI RAATTY 2 cut(s) 30, 359
XceI RCATGY 2 cut(s) 14, 607
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.