Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000005

Orthogroup ID

OG0001701

Physical Location & Seq

Genomic Coordinates Forward (+)

63907744 .. 63914670

Gene Structure 6927 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_5G00384620.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2298 bp

ATGGTGACGCCGCGGAAGAAGAACCCTAAGGCCTCTCCGAAATCGAAGAAGACAACCGATCCGGAGCAAGCTTCACCGGAGGTACCTGCTTCTTCCACGGTCTTTGACGGCCGAGAGATGAATCCATCGGCGAGGAAGAACAAGTCCAATGCGCCTCCCGCCTCGGCTCCGGAACCTAAGAAGTCGAAGGGAACCAAGCCGAAGGCTGGTCCTTGTTCCATTGTCGTCGACGGTCCGGAGCCTACGTTCGTTGGCGAACCGCTTACCGACGAGGAGGCTCGGCGGAGATGGCCAGAATCGTACCGAAAGGAGAAGCAAGTTGCTGGACCAAGCAGCTCTAAGGGAGATGAAGATTCTCTTCAGGCTCGTTCTCACTTTACAATGGCTGAAGTGGATGGTATAAGATATGATCTATATGATGATGCTCATGTTCTGGCTGGAGAAGGAGAGCCACCATACATTTGCAAGATAGTTGAGATGTTTGAGGCAGTTGATGGGCAGTTATATTTTACAGCTCAATGGTACTACCGAGGACAAGACACTGTCATCAAAGACTGTGTTGAGATTGATAGTAGACGTGTTTTCTTCTCAGATGTGAAGGACCGCAATCCTTTGGAATGTCTTCTTGAAAAGATTACTATTGTTAGATTGGCTCTAAATGTTGATCCTGATGTTAGGAACAAACTTATTCAAGAGTGCAAGTATTATTGTGACACCAAATACTTGTTGCCTCATTCAACTTTTGTCAATTTACTACCAGGTTATTGTCTTTCATTTTTTACTGTCAAGAAGTTGGTTCATGCCTCATTTGGAACAAACCAATGTTCACAAGGACATAAAATTTTTACGTGTATTGAACTCACAGAAAATATGCAATCGGGAAGTCATATTCCATCAACAATTTCTTGTGGGGTTGTTGGTGAATCTTGTCATGTTAACTCAGAACTTGTGAAAAGCTCCATAGTCAGGGAGCAATGCTTACAAGAACTTAAATTGCTGGACTTATATGCTGGGTGTGGTGGGATGTCAACTGGCCTATGTTTGGGTGCCCGATTATCCAACGTAAATCTTGTCACGAGATGGGCTGTTGATATAAATGAATATGCACTCAAAAGTCTAAAGCTTAATCATCCAGAAACTGAGGTTAGAAAGGAATATGCGGAAAACTTTCTGATTTTGCTCAAGAAGTGGAAGGGACTATGCATTTCTTTTAACTTGGTTGAGGGCAACAATTCAGAGATGCCGGTCAATTTCTTTGCAACAAATAACCCTTCAGATGAAGAAGAGGATGACGACCAGAATAAGGATAATGCTGAAGATTCTGAGGTTTATGAAGTCGAAAAAATACTTGATATATGTTATGGGGAACCAGAAGAGAAAGATAGGGGGCTGTACTTCAAGGTTCATTGGAAGGGCTATGGTTCAGATGAAGATACTTGGGAGCCTATTGAGTCACTGAGGGATTGCAAGGATAGTGTACAAGAATTTGTTTCTCGTGGCTTCAGATCAAAAATGTTGCCGTTACCTGGAGATGTTGATGTGGTGTGTGGTGGACCACCTTGCCAGGGAATAAGCGGATTCAACCGATTTAGGGACAAGGAAAATCCTTTGAATGATAAGAAAAATGAACAGGTGGTTGTTTTCATGGACATAGTACAGTACCTTAAGCCTAAGTATGTCTTGATGGAAAACGTGGTCGACCTTTTGAAATTTGCGGAAGGGTTCCTTGGGCGATATGCCATTGGACGTCTTGTTGATATGAACTATCAGGCAAGGATCGGTATGATGTGTGCAGGAGCATATGGTCTTCCTCAATTCCGTATGCGGGTTTTTCTATGGGGGGCCTGTCCTACTGAGCGGTTGCCACAATACCCGCTTCCTACCCATGATGTTGTTGTCAAGGGTCATACTCCAACTGAATGGGAGCCTTTGACAGTGAATGCTGACGATTATGCTCGTATATGTCGCATTCCCAAAGAGAAGGGTGCAAATTTTAGAAACCTAACTGGTGTTCGAGTGCGTCCTGATAAAAAAGTTGAATGGAATCCTGATGTAGACAGGGTTTATTTGGACTCTGGCGCTCCTTTGGTCCCTGACTATGCTATGTCATTTGTGAAAGGGAAATCATCAAAATACATTCAAGTTGGGAATGCCGTAGCAGTTCCTGTCGCGCGTGCTCTGGGATATGCACTAGGGCGTGCTCTCAATGGTTTAGTTGATCATCGTTCTATGTTTGAGTTGCCTGATGGGTTCCCCAATCATCTGGAAGATATTCCTTCTCCAAAAGCTAGAGAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00270 ko01100 ko05206 map00270 map01100 map05206

Pfam Domains

Protein Families

BAH Chromo DNA_methylase

Protein Analysis

765

Amino Acids

85.97

Weight (kDa)

5.69

Isoelectric Point (pI)

39.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
BAH	PF01426	148 - 241	1.1e-10	BAH domain
Chromo	PF00385	444 - 499	1e-13	Chromo (CHRromatin Organisation MOdifier) domain
DNA_methylase	PF00145	508 - 627	4.9e-17	C-5 cytosine-specific DNA methylase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001701)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G69770
fragaria_vesca	FvH4_1g00970 FvH4_2g39560 FvH4_2g39560 FvH4_2g39560
malus_domestica	MD03G1011100.v1.1 MD03G1011200.v1.1 MD11G1012800.v1.1 MD11G1013000.v1.1 MD11G1013100.v1.1 MD11G1013200.v1.1 MD11G1013700.v1.1
prunus_persica	Prupe.6G011600_v2.0.a1 Prupe.6G011600_v2.0.a1
pyrus_communis	pycom03g00990 pycom11g00840 pycom11g00870 pycom11g00900
rosa_chinensis	RchiOBHm_Chr2g0085701 RchiOBHm_Chr4g0398431 RchiOBHm_Chr4g0444071
rosa_laevigata	RLG00000005861 RLG00000009362 RLG00000015713
rosa_multiflora	Rmu_co8144248.1_g000001 Rmu_sc0009759.1_g000011
rosa_roxburghii	Rroxscaffold_2G00155110 Rroxscaffold_5G00343400 Rroxscaffold_5G00384620
rosa_rugosa	Rorug01G0462700 Rorug04G0007900 Rorug04G0349300 Rorug04G0349400
rosa_samantha	Rh2AG011600 Rh2BG012200 Rh2CG013000 Rh2DG014000 Rh4AG086100 Rh4AG410100 Rh4BG083000 Rh4BG421200 Rh4CG093900 Rh4CG435600 Rh4DG077800 Rh4DG416500
rosa_wichuraiana	Rw2G001030 Rw4G007200 Rw4G035230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1750
Acc36I	ACCTGC	1 cut(s)	94
Acc65I	GGTACC	1 cut(s)	82
AccB1I	GGYRCC	2 cut(s)	82, 1046
AccBSI	CCGCTC	1 cut(s)	1858
AccI	GTMKAC	4 cut(s)	228, 574, 1698, 2055
AccII	CGCG	3 cut(s)	13, 2171, 2173
AccIII	TCCGGA	3 cut(s)	61, 169, 235
AclWI	GGATC	3 cut(s)	53, 659, 1784
AcoI	YGGCCR	2 cut(s)	109, 290
AcsI	RAATTY	4 cut(s)	840, 1484, 1709, 1990
AcuI	CTGAAG	5 cut(s)	344, 408, 1257, 1335, 1486
AcyI	GRCGYC	2 cut(s)	8, 1747
AfaI	GTAC	7 cut(s)	84, 302, 524, 1394, 1479, 1656, 1661
AfiI	CCNNNNNNNGG	8 cut(s)	206, 966, 1042, 1303, 1526, 1565, 1591, 1825
AflII	CTTAAG	1 cut(s)	1664
AflIII	ACRYGT	2 cut(s)	577, 848
AjiI	CACGTC	1 cut(s)	578
AjnI	CCWGG	3 cut(s)	757, 1525, 1563
AjuI	GAANNNNNNNTTGG	2 cut(s)	1710, 1742
AloI	GAACNNNNNNTCC	2 cut(s)	230, 262
AluBI	AGCT	6 cut(s)	71, 336, 515, 957, 1123, 2288
AluI	AGCT	6 cut(s)	71, 336, 515, 957, 1123, 2288
Alw21I	GWGCWC	2 cut(s)	2179, 2203
AlwI	GGATC	3 cut(s)	53, 659, 1784
AlwNI	CAGNNNCTG	2 cut(s)	1139, 2165
Aor13HI	TCCGGA	3 cut(s)	61, 169, 235
AoxI	GGCC	5 cut(s)	30, 109, 290, 1033, 1842
ApeKI	GCWGC	1 cut(s)	333
ApoI	RAATTY	4 cut(s)	840, 1484, 1709, 1990
Asp700I	GAANNNNTTC	3 cut(s)	621, 1167, 2271
Asp718I	GGTACC	1 cut(s)	82
AspLEI	GCGC	3 cut(s)	154, 2081, 2173
AspS9I	GGNCC	7 cut(s)	209, 233, 326, 601, 1553, 1842, 2089
AsuHPI	GGTGA	3 cut(s)	16, 66, 932
AvaII	GGWCC	6 cut(s)	209, 233, 326, 601, 1553, 2089
AxyI	CCTNAGG	1 cut(s)	27
BaeGI	GKGCMC	1 cut(s)	1051
BalI	TGGCCA	1 cut(s)	292
BanI	GGYRCC	2 cut(s)	82, 1046
BauI	CACGAG	2 cut(s)	1075, 1494
BbsI	GAAGAC	3 cut(s)	56, 614, 1799
Bbv12I	GWGCWC	2 cut(s)	2179, 2203
BbvI	GCAGC	1 cut(s)	345
BccI	CCATC	8 cut(s)	133, 282, 389, 488, 901, 1074, 1678, 2240
BceAI	ACGGC	3 cut(s)	124, 1504, 2138
BciT130I	CCWGG	3 cut(s)	759, 1527, 1565
BclI	TGATCA	1 cut(s)	2218
BfaI	CTAG	2 cut(s)	2192, 2289
BfoI	RGCGCY	1 cut(s)	2082
BfrI	CTTAAG	1 cut(s)	1664
BfuAI	ACCTGC	1 cut(s)	94
BisI	GCNGC	2 cut(s)	11, 334
BlsI	GCNGC	2 cut(s)	12, 335
Bme1390I	CCNGG	3 cut(s)	759, 1527, 1565
Bme18I	GGWCC	6 cut(s)	209, 233, 326, 601, 1553, 2089
BmgBI	CACGTC	1 cut(s)	578
BmgT120I	GGNCC	7 cut(s)	209, 233, 326, 601, 1553, 1842, 2089
BmrFI	CCNGG	3 cut(s)	759, 1527, 1565
BmsI	GCATC	2 cut(s)	412, 1230
BpiI	GAAGAC	3 cut(s)	56, 614, 1799
BpmI	CTGGAG	2 cut(s)	459, 1548
BpuEI	CTTGAG	1 cut(s)	1166
BsaAI	YACGTR	1 cut(s)	849
BsaHI	GRCGYC	2 cut(s)	8, 1747
BsaJI	CCNNGG	6 cut(s)	11, 96, 162, 529, 1564, 1726
BsaWI	WCCGGW	4 cut(s)	61, 76, 169, 235
BsaXI	ACNNNNNCTCC	2 cut(s)	230, 260
Bsc4I	CCNNNNNNNGG	8 cut(s)	206, 966, 1042, 1303, 1526, 1565, 1591, 1825
Bse118I	RCCGGY	1 cut(s)	1243
Bse1I	ACTGG	2 cut(s)	1036, 2011
Bse21I	CCTNAGG	1 cut(s)	27
Bse3DI	GCAATG	1 cut(s)	980
BseAI	TCCGGA	3 cut(s)	61, 169, 235
BseBI	CCWGG	3 cut(s)	759, 1527, 1565
BseDI	CCNNGG	6 cut(s)	11, 96, 162, 529, 1564, 1726
BseGI	GGATG	4 cut(s)	400, 1029, 1129, 1294
BseLI	CCNNNNNNNGG	8 cut(s)	206, 966, 1042, 1303, 1526, 1565, 1591, 1825
BseMI	GCAATG	1 cut(s)	980
BseMII	CTCAG	6 cut(s)	603, 954, 1131, 1314, 1448, 1845
BseNI	ACTGG	2 cut(s)	1036, 2011
BseRI	GAGGAG	1 cut(s)	287
BseSI	GKGCMC	1 cut(s)	1051
BseX3I	CGGCCG	1 cut(s)	109
BseXI	GCAGC	1 cut(s)	345
BseYI	CCCAGC	1 cut(s)	1010
BsgI	GTGCAG	1 cut(s)	1812
Bsh1236I	CGCG	3 cut(s)	13, 2171, 2173
Bsh1285I	CGRYCG	1 cut(s)	112
BshFI	GGCC	5 cut(s)	32, 111, 292, 1035, 1844
BshNI	GGYRCC	2 cut(s)	82, 1046
BsiEI	CGRYCG	1 cut(s)	112
BsiHKAI	GWGCWC	2 cut(s)	2179, 2203
BsiSI	CCGG	5 cut(s)	62, 77, 170, 236, 1244
BslFI	GGGAC	3 cut(s)	1209, 1607, 2075
BslI	CCNNNNNNNGG	8 cut(s)	206, 966, 1042, 1303, 1526, 1565, 1591, 1825
BsmFI	GGGAC	3 cut(s)	1209, 1607, 2075
BsmI	GAATGC	3 cut(s)	1945, 1968, 2155
BsnI	GGCC	5 cut(s)	32, 111, 292, 1035, 1844
Bsp1286I	GDGCHC	3 cut(s)	1051, 2179, 2203
Bsp13I	TCCGGA	3 cut(s)	61, 169, 235
Bsp1407I	TGTACA	1 cut(s)	1477
Bsp143I	GATC	6 cut(s)	58, 409, 664, 1505, 1776, 2218
BspANI	GGCC	5 cut(s)	32, 111, 292, 1035, 1844
BspCNI	CTCAG	6 cut(s)	602, 953, 1132, 1315, 1449, 1846
BspEI	TCCGGA	3 cut(s)	61, 169, 235
BspFNI	CGCG	3 cut(s)	13, 2171, 2173
BspMI	ACCTGC	1 cut(s)	94
BspPI	GGATC	3 cut(s)	53, 659, 1784
BspT107I	GGYRCC	2 cut(s)	82, 1046
BspTI	CTTAAG	1 cut(s)	1664
BsrBI	CCGCTC	1 cut(s)	1858
BsrDI	GCAATG	1 cut(s)	980
BsrFI	RCCGGY	1 cut(s)	1243
BsrGI	TGTACA	1 cut(s)	1477
BsrI	ACTGG	2 cut(s)	1036, 2011
BssAI	RCCGGY	1 cut(s)	1243
BssECI	CCNNGG	6 cut(s)	11, 96, 162, 529, 1564, 1726
BssMI	GATC	6 cut(s)	58, 409, 664, 1505, 1776, 2218
BssNI	GRCGYC	2 cut(s)	8, 1747
BssSI	CACGAG	2 cut(s)	1075, 1494
BssT1I	CCWWGG	1 cut(s)	1726
Bst2BI	CACGAG	2 cut(s)	1075, 1494
Bst2UI	CCWGG	3 cut(s)	759, 1527, 1565
Bst4CI	ACNGT	7 cut(s)	100, 233, 544, 557, 784, 1659, 1936
Bst6I	CTCTTC	3 cut(s)	363, 1278, 1368
BstACI	GRCGYC	2 cut(s)	8, 1747
BstAFI	CTTAAG	1 cut(s)	1664
BstAUI	TGTACA	1 cut(s)	1477
BstBAI	YACGTR	1 cut(s)	849
BstC8I	GCNNGC	3 cut(s)	69, 2175, 2199
BstDSI	CCRYGG	2 cut(s)	11, 96
BstF5I	GGATG	4 cut(s)	400, 1029, 1129, 1294
BstFNI	CGCG	3 cut(s)	13, 2171, 2173
BstH2I	RGCGCY	1 cut(s)	2082
BstHHI	GCGC	3 cut(s)	154, 2081, 2173
BstKTI	GATC	6 cut(s)	61, 412, 667, 1508, 1779, 2221
BstMBI	GATC	6 cut(s)	58, 409, 664, 1505, 1776, 2218
BstMCI	CGRYCG	1 cut(s)	112
BstMWI	GCNNNNNNNGC	2 cut(s)	158, 289
BstNI	CCWGG	3 cut(s)	759, 1527, 1565
BstSCI	CCNGG	3 cut(s)	757, 1525, 1563
BstSLI	GKGCMC	1 cut(s)	1051
BstUI	CGCG	3 cut(s)	13, 2171, 2173
BstV1I	GCAGC	1 cut(s)	345
BstV2I	GAAGAC	3 cut(s)	56, 614, 1799
BstXI	CCANNNNNNTGG	2 cut(s)	1920, 2263
BstZI	CGGCCG	1 cut(s)	109
Bsu36I	CCTNAGG	1 cut(s)	27
BsuRI	GGCC	5 cut(s)	32, 111, 292, 1035, 1844
BtgI	CCRYGG	2 cut(s)	11, 96
BtrI	CACGTC	1 cut(s)	578
BtsCI	GGATG	4 cut(s)	400, 1029, 1129, 1294
BtsIMutI	CAGTG	3 cut(s)	540, 1454, 1941
BveI	ACCTGC	1 cut(s)	94
Cac8I	GCNNGC	3 cut(s)	69, 2175, 2199
CaiI	CAGNNNCTG	2 cut(s)	1139, 2165
CfoI	GCGC	3 cut(s)	154, 2081, 2173
Cfr10I	RCCGGY	1 cut(s)	1243
Cfr13I	GGNCC	7 cut(s)	209, 233, 326, 601, 1553, 1842, 2089
Cfr42I	CCGCGG	1 cut(s)	14
CpoI	CGGWCCG	1 cut(s)	233
CseI	GACGC	2 cut(s)	16, 2009
CsiI	ACCWGGT	1 cut(s)	757
Csp6I	GTAC	7 cut(s)	83, 301, 523, 1393, 1478, 1655, 1660
CspCI	CAANNNNNGTGG	2 cut(s)	85, 120
CspI	CGGWCCG	1 cut(s)	233
CviAII	CATG	5 cut(s)	428, 800, 932, 1645, 1886
CviQI	GTAC	7 cut(s)	83, 301, 523, 1393, 1478, 1655, 1660
DpnI	GATC	6 cut(s)	60, 411, 666, 1507, 1778, 2220
DpnII	GATC	6 cut(s)	58, 409, 664, 1505, 1776, 2218
EaeI	YGGCCR	2 cut(s)	109, 290
EagI	CGGCCG	1 cut(s)	109
Eam1104I	CTCTTC	3 cut(s)	363, 1278, 1368
EarI	CTCTTC	3 cut(s)	363, 1278, 1368
EciI	GGCGGA	1 cut(s)	298
EclXI	CGGCCG	1 cut(s)	109
Eco130I	CCWWGG	1 cut(s)	1726
Eco147I	AGGCCT	1 cut(s)	32
Eco47I	GGWCC	6 cut(s)	209, 233, 326, 601, 1553, 2089
Eco52I	CGGCCG	1 cut(s)	109
Eco57I	CTGAAG	5 cut(s)	344, 408, 1257, 1335, 1486
Eco81I	CCTNAGG	1 cut(s)	27
EcoO109I	RGGNCCY	1 cut(s)	1842
EcoRII	CCWGG	3 cut(s)	757, 1525, 1563
EcoT14I	CCWWGG	1 cut(s)	1726
EcoT22I	ATGCAT	1 cut(s)	1205
ErhI	CCWWGG	1 cut(s)	1726
FaeI	CATG	5 cut(s)	431, 803, 935, 1648, 1889
FalI	AAGNNNNNCTT	6 cut(s)	342, 374, 1664, 1696, 1710, 1742
FaqI	GGGAC	3 cut(s)	1209, 1607, 2075
FatI	CATG	5 cut(s)	427, 799, 931, 1644, 1885
FauI	CCCGC	3 cut(s)	166, 1818, 1881
FauNDI	CATATG	1 cut(s)	1801
FbaI	TGATCA	1 cut(s)	2218
FblI	GTMKAC	4 cut(s)	228, 574, 1698, 2055
Fnu4HI	GCNGC	2 cut(s)	11, 334
FokI	GGATG	4 cut(s)	407, 1036, 1116, 1301
Fsp4HI	GCNGC	2 cut(s)	11, 334
FspBI	CTAG	2 cut(s)	2192, 2289
GlaI	GCGC	3 cut(s)	153, 2080, 2172
GluI	GCNGC	2 cut(s)	11, 334
GsaI	CCCAGC	1 cut(s)	1014
GsuI	CTGGAG	2 cut(s)	459, 1548
HaeII	RGCGCY	1 cut(s)	2082
HaeIII	GGCC	5 cut(s)	32, 111, 292, 1035, 1844
HapII	CCGG	5 cut(s)	62, 77, 170, 236, 1244
HgaI	GACGC	2 cut(s)	16, 2009
HhaI	GCGC	3 cut(s)	154, 2081, 2173
Hin1I	GRCGYC	2 cut(s)	8, 1747
Hin1II	CATG	5 cut(s)	431, 803, 935, 1648, 1889
Hin6I	GCGC	3 cut(s)	152, 2079, 2171
HinP1I	GCGC	3 cut(s)	152, 2079, 2171
HincII	GTYRAC	4 cut(s)	229, 937, 1029, 1699
HindII	GTYRAC	4 cut(s)	229, 937, 1029, 1699
HindIII	AAGCTT	2 cut(s)	69, 1121
HinfI	GANTC	9 cut(s)	121, 296, 353, 923, 1319, 1451, 1578, 2044, 2072
HpaI	GTTAAC	1 cut(s)	937
HpaII	CCGG	5 cut(s)	62, 77, 170, 236, 1244
HphI	GGTGA	3 cut(s)	16, 66, 932
Hpy166II	GTNNAC	9 cut(s)	229, 575, 827, 937, 1029, 1478, 1553, 1699, 2056
Hpy188I	TCNGA	9 cut(s)	39, 592, 943, 1173, 1237, 1276, 1324, 1426, 1505
Hpy8I	GTNNAC	9 cut(s)	229, 575, 827, 937, 1029, 1478, 1553, 1699, 2056
Hpy99I	CGWCG	3 cut(s)	230, 233, 272
HpyCH4III	ACNGT	7 cut(s)	100, 233, 544, 557, 784, 1659, 1936
HpyCH4IV	ACGT	6 cut(s)	245, 577, 848, 1062, 1692, 1747
HpyF10VI	GCNNNNNNNGC	2 cut(s)	158, 289
HpySE526I	ACGT	6 cut(s)	245, 577, 848, 1062, 1692, 1747
Hsp92I	GRCGYC	2 cut(s)	8, 1747
Hsp92II	CATG	5 cut(s)	431, 803, 935, 1648, 1889
HspAI	GCGC	3 cut(s)	152, 2079, 2171
Kpn2I	TCCGGA	3 cut(s)	61, 169, 235
KpnI	GGTACC	1 cut(s)	86
Ksp22I	TGATCA	1 cut(s)	2218
KspAI	GTTAAC	1 cut(s)	937
KspI	CCGCGG	1 cut(s)	14
Kzo9I	GATC	6 cut(s)	58, 409, 664, 1505, 1776, 2218
LmnI	GCTCC	9 cut(s)	64, 172, 238, 962, 970, 1441, 1796, 1924, 2086
Lsp1109I	GCAGC	1 cut(s)	345
LweI	GCATC	2 cut(s)	412, 1230
MabI	ACCWGGT	1 cut(s)	757
MaeI	CTAG	2 cut(s)	2192, 2289
MaeII	ACGT	6 cut(s)	245, 577, 848, 1062, 1692, 1747
MaeIII	GTNAC	5 cut(s)	4, 710, 1072, 1452, 1521
MalI	GATC	6 cut(s)	60, 411, 666, 1507, 1778, 2220
MbiI	CCGCTC	1 cut(s)	1858
MboI	GATC	6 cut(s)	58, 409, 664, 1505, 1776, 2218
MhlI	GDGCHC	3 cut(s)	1051, 2179, 2203
MlsI	TGGCCA	1 cut(s)	292
MluNI	TGGCCA	1 cut(s)	292
MlyI	GAGTC	2 cut(s)	1460, 2066
MmeI	TCCRAC	2 cut(s)	1083, 1937
Mox20I	TGGCCA	1 cut(s)	292
Mph1103I	ATGCAT	1 cut(s)	1205
MroI	TCCGGA	3 cut(s)	61, 169, 235
MroXI	GAANNNNTTC	3 cut(s)	621, 1167, 2271
MscI	TGGCCA	1 cut(s)	292
MseI	TTAA	5 cut(s)	936, 990, 1125, 1212, 1665
Msp20I	TGGCCA	1 cut(s)	292
MspA1I	CMGCKG	1 cut(s)	13
MspCI	CTTAAG	1 cut(s)	1664
MspI	CCGG	5 cut(s)	62, 77, 170, 236, 1244
MspR9I	CCNGG	3 cut(s)	759, 1527, 1565
Mva1269I	GAATGC	3 cut(s)	1945, 1968, 2155
MvaI	CCWGG	3 cut(s)	759, 1527, 1565
MvnI	CGCG	3 cut(s)	13, 2171, 2173
MwoI	GCNNNNNNNGC	2 cut(s)	158, 289
NdeI	CATATG	1 cut(s)	1801
NdeII	GATC	6 cut(s)	58, 409, 664, 1505, 1776, 2218
NlaIII	CATG	5 cut(s)	431, 803, 935, 1648, 1889
NmeAIII	GCCGAG	3 cut(s)	137, 143, 259
NmuCI	GTSAC	4 cut(s)	4, 710, 1072, 1452
NsiI	ATGCAT	1 cut(s)	1205
PceI	AGGCCT	1 cut(s)	32
PctI	GAATGC	3 cut(s)	1945, 1968, 2155
PdmI	GAANNNNTTC	3 cut(s)	621, 1167, 2271
PfeI	GAWTC	7 cut(s)	121, 296, 353, 923, 1319, 1578, 2044
PflFI	GACNNNGTC	1 cut(s)	542
PkrI	GCNGC	2 cut(s)	12, 335
PleI	GAGTC	2 cut(s)	1459, 2066
PpsI	GAGTC	2 cut(s)	1459, 2066
Ppu21I	YACGTR	1 cut(s)	849
Psp6I	CCWGG	3 cut(s)	757, 1525, 1563
PspFI	CCCAGC	1 cut(s)	1010
PspGI	CCWGG	3 cut(s)	757, 1525, 1563
PspPI	GGNCC	7 cut(s)	209, 233, 326, 601, 1553, 1842, 2089
PstNI	CAGNNNCTG	2 cut(s)	1139, 2165
PsyI	GACNNNGTC	1 cut(s)	542
RsaI	GTAC	7 cut(s)	84, 302, 524, 1394, 1479, 1656, 1661
RsaNI	GTAC	7 cut(s)	83, 301, 523, 1393, 1478, 1655, 1660
Rsr2I	CGGWCCG	1 cut(s)	233
RsrII	CGGWCCG	1 cut(s)	233
SacII	CCGCGG	1 cut(s)	14
SalI	GTCGAC	2 cut(s)	227, 1697
SaqAI	TTAA	5 cut(s)	936, 990, 1125, 1212, 1665
SatI	GCNGC	2 cut(s)	11, 334
Sau3AI	GATC	6 cut(s)	58, 409, 664, 1505, 1776, 2218
Sau96I	GGNCC	7 cut(s)	209, 233, 326, 601, 1553, 1842, 2089
SchI	GAGTC	2 cut(s)	1460, 2066
ScrFI	CCNGG	3 cut(s)	759, 1527, 1565
SduI	GDGCHC	3 cut(s)	1051, 2179, 2203
SexAI	ACCWGGT	1 cut(s)	757
SfaNI	GCATC	2 cut(s)	412, 1230
Sfr303I	CCGCGG	1 cut(s)	14
SgrBI	CCGCGG	1 cut(s)	14
SgrDI	CGTCGACG	1 cut(s)	227
SinI	GGWCC	6 cut(s)	209, 233, 326, 601, 1553, 2089
SmlI	CTYRAG	2 cut(s)	1181, 1664
SmoI	CTYRAG	2 cut(s)	1181, 1664
SseBI	AGGCCT	1 cut(s)	32
SspMI	CTAG	2 cut(s)	2192, 2289
StuI	AGGCCT	1 cut(s)	32
StyD4I	CCNGG	3 cut(s)	757, 1525, 1563
StyI	CCWWGG	1 cut(s)	1726
TaaI	ACNGT	7 cut(s)	100, 233, 544, 557, 784, 1659, 1936
TaiI	ACGT	6 cut(s)	248, 580, 851, 1065, 1695, 1750
TaqI	TCGA	6 cut(s)	44, 185, 228, 1338, 1698, 2014
TatI	WGTACW	3 cut(s)	1392, 1477, 1654
TauI	GCSGC	1 cut(s)	13
TfiI	GAWTC	7 cut(s)	121, 296, 353, 923, 1319, 1578, 2044
Tru1I	TTAA	5 cut(s)	936, 990, 1125, 1212, 1665
Tru9I	TTAA	5 cut(s)	936, 990, 1125, 1212, 1665
TscAI	CASTG	3 cut(s)	547, 1461, 1941
TseFI	GTSAC	4 cut(s)	4, 710, 1072, 1452
TseI	GCWGC	1 cut(s)	333
Tsp45I	GTSAC	4 cut(s)	4, 710, 1072, 1452
TspGWI	ACGGA	1 cut(s)	1808
TspRI	CASTG	3 cut(s)	547, 1461, 1941
Tth111I	GACNNNGTC	1 cut(s)	542
Vha464I	CTTAAG	1 cut(s)	1664
VpaK11BI	GGWCC	6 cut(s)	209, 233, 326, 601, 1553, 2089
XapI	RAATTY	4 cut(s)	840, 1484, 1709, 1990
XmiI	GTMKAC	4 cut(s)	228, 574, 1698, 2055
XmnI	GAANNNNTTC	3 cut(s)	621, 1167, 2271
XspI	CTAG	2 cut(s)	2192, 2289
ZraI	GACGTC	1 cut(s)	1748
Zsp2I	ATGCAT	1 cut(s)	1205

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.