RLG00000015713

DNA (cytosine-5)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001701
Physical Location & Seq
Forward (+)
910042 .. 919209
9168 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015713

Sequence Viewer

Length: 3930 bp
ATGGCCACCAAGCGCAAGTCGAAGCGATCTCCGGCTTCTTCTTCCGCCGCGAAGAGGCTGAAGCAGGGGGAGAAAGATGTCTTATTGCCGGAGAGTAGTGAAATCGAAACCGAAACCGAAACCTCAACGGTGACGAAGGCGAAGCCGAAGCGATCTAAGGCTGGATCTTCATCCGCATCTGTGAAGTTGAAGGAGCCGGCGGCGGTGGTGGTGGAGGAAGAGTCGGTACTGGAGAGTACCGAAACGGCCGAGAAGGTTTCGGAGAGTAGCGAAACAGCTACAATGGTGGCGAGTGCGCCGAAGTCGAGGAAGTACAGTAAGAAGGAGAGTAAGGAGAGCGGTGGTTCTGAGGCTAATAATGTGGCAGATGAGGATGTGGTGATGTCTGAGGCTAGGTTTCTCGGTGAGCCGGTTGAACCCGAGGAGGCTCGCCGGAGATGGCCCCACCGATATGCTACGCCTCCGACTCAGAAGAAGGCAAACAACTCCAACGATGATGAAGATTTTCTTGAGGGTCGTCGACACTTCACAAAGGCAGAGGTTGATGGATGTATCTATGATCTTTATGATGATGCACATGTAAAAGCCGGCAAAGGAGAACAACCTTACATTTGTAAAATTATAGAGATGTTTGAGGCAGTTGATGGGCTACCCTATTTTACTGCTCAGTGGTTCTACAGAGCGAAAGATACTGTCATAGAAGGTTGCTTCGATATTGATTCAAGGCGCGTATTTTATTCAGATGTTACAAATTACAATGAGTTGGAATGTCTTCTTGGGAAACTGGATATTGCGAGATTCGGTTTAGATAGTGACCCAGATGTAAAGTGCAAACTGATTGAGAGCTGTACCTATTATTGTGACACTAAATATCTGTTGCCTTATTCCACCTTTGTCAACTTCCCACCTGAAAATATGCAGGCTGGAAGTGATAACTCATCAACAATTTCCAGTGAGATTGACATTAGTTGTTCTCGTGCGGTTAACTCAGAACTTGAAAAAACTTCCACAGTTGTGGAGCAAGGCAAACCAGAAGCAACTGTACTTGACTTGTATTCTGGCTGTGGAGGGATGTCTACTGGCCTATGCCTTGGGGCAAACTCGGCAAACTTAAATCTTGTTACGAGATGGGCTGTGGATCTGAATGAACATGCTATAAAAAGTCTGAAGATAAACCATGAAGAAACTGAGGTTCGGGTAGAATCTGCGGATGATTTTCTCTCATTACTGAAAAAGTGGAAGGGACTTTGTGTTCATTTTAAAGTGGTTAAACCTGATGGTTCAGAAACACCGTTCAGTGTATTCACAACAAAAGATGCCAAAGAGGAAGAGGAGGATGTGGAGGCAGAGGATGATGATGATGATGCAGAATCTGAAGTTTATGAAGTTGAAAAAATACTTGGTATACGTTATGGAAAAGCCAAAAATGGAGAAGAGAAGGAATTGTTGTTTAAGGTCAAATGGAAAGGATATGGAGCTGAGTATGATACTTGGGAGCCCCTAGATTTACTAACGGGTTGTGAGGAGAGCATACAGGAATTTGTTTCTCATGTATACCAGTCCAAACTGTTGCCATTACCTGGTGACGTGGATGTTGTGTGTGGTGGTCCTCCATGCCAAGGAATTAGCGGATTCAATAGGTTCAGGAACTCTGAAAGTCCATTAGATGACGAGAAGAACAAGCAATTGGCTGTCTTCATGGACATAGTAGATTATCTCAAGCCCAAGTATGTGCTGATGGAAAATGTAGTGGATCTTTTGAAATTTGCTGATGGGTTTTTAGGACGGTATGCTTTGGGGAGGTTAGTTGGAATGAACTACCAAGCAAGGATGGGTATGATGTGCGCAGGTGCATATGGTCTACCTCAATTCCGTATGCGGGTATTTTATTGGGGGGCCCTTCCTACTGAGAGGTTGCCTCAATTCCCACTGCCAACCCATGATGTAGTGTTTAGGGGTCAGATTCCTACTAAATGGGAGCAAAACTGTGTTGCTTACAATGAAGGCCATGATCATACATTGGGAAGAAAGCTTTTGTTGGAGGATGCACTCTCAGATCTTCCTGAGGTTGGAAACAATGAAAACCGTGACGTAATGCCTTATGGAAAGCAACCTCAGACAGAATTTCAGAAATTTATTCGGCTTAGCAAAGACGGTTTTCTGGGGACTTCAAAGAAGTTGCACTCAAATGAGATGCTTTATGATCACCGCCCTCTGAAATTGAATGCTGATGATCATGAACGTGTTTGTCGTGTCCCTAAGGAAAAGGGTGCGAACTTTAGGAATCTACCTGGGGTTCTTGTTGGTGCTGATAATAAAGTAATGTGGGATCCAGAAGTAGAGAGGGTTTTATTAAAATCTGGGAAACCTTTGGTCCCTGATTATGCAATGTCATTTGTGAAAGGACATTCATCAAAACCTTTTGGACGTTTATGGTGGGATGAGATTGTTTCCACAGTTGTTACCCGGATGAATAAGATACTTGATGTTTGGGGATATATGCAAATTGGAAATGCTGTAGCTGTTCCTGTTTCACGTGCACTGGGTTACACATTAGGTTTGGCATATAAGGGTTCTGCTGGTGAGGATTCTTTGTTTGAGCTGCCCAAAGAAATGCATTTGCCTAACGGATGTTTTGAAGATGGAGATTATGAGATAAAGCGAAAATTTACCTGCAATCCAACTAAAGACCCAAATCTACCTAGCTCGAAATTCCGGAGTAACGCGTTTTACAAGAGGAGAGCACTAGTCACTAGTGCATGGCCCCAGCTATTTGAATATCTTGGCCACTCTGTTGACCTCAAAATCACCTTCACCAGTTCTGCCTACCTTAATCTAATGCAGCCGCTGCTCGAGTCAGGGGGAAGAATGGAGGAGGATTCACCAAATCCTCCCGTTTCATTGCATGCCCGGATTTCTGCGCTTAAGACAATGGCTTGGGAAGAATACAAAAGCAAGCCAATCTCGCATTGGATTCTTCTACTTCTAAGCAGTGCGGCAATGCTTGTAGCGTTTCCTGCATCTAGCCTTCTATCTCGTATCTATTTTGCCAATGGCGGCACTAGCAAGTGGATCATTTCATGGGTGGCTGTTGCAGGGTGGCCTCTGACTGCTTTGATCTTACTGCCTACGTACTTCTTCTGCAAAACCTCTCCTACCCCTCTGAATTTAAAACTTACATTATCTTATGCTTTCCTAGGTTTCCTAAGTGCTGCTGACAATCTTATGTATGCATATGCATATGCATACCTCCCAGCATCTACTGCTTCTCTTCTTGCATCATCATCTCTTGTATTTTCTGCATTGTTTGGGTATATACTGGTGAAGAACAAACTCAATGCTTCAATAGTAAATGCTATTGTGATCATTACTGCTGCTATGACCATCATTGCATTGGATTCTGATTCAGACAGATATGACTATGTCACTGAGAAGCAATACATTCTAGGTTTTGTGTGGGATATTTTGGGATCTGCTCTCCATGGGCTCATTTTTGCTCTCTCAGAGCTAGTTTTTGTGAAGCTACTTGGTAGAAGGTCCTTCCATGTTGTGCTGGAGCAACAACTCGGGGTTTCCCTATTTGCGTTTCTGTTTACCACGATTGGGGTCATTTGGAATAAGGATTTTCAGGGAATGGCATCTGAGGCGAGAAGTTTCGAGGGTGGTAAATCTTCTTATTACCTTGTTCTCATTTGGAGTACAATCACTTTCCAGTTGGGGGTGTTAGGAGCTACTGCTGTGCTTTTTCTGTCTTCCACCGTTCTCGCTGGTGTTCTCAATGCAGTAAGGGTGCCACTCACAAGCATTGCAGCTGTGATATTGTTAAACGATCCCATGAGCGGCTTAAAGATCCTCTCTTTGATAGTTACATTTTGGGGATTCGGCTGCTACATCTATGGCAGCTCCTCTGGAAGCAGAGATCCTACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko01100 ko05206 map00270 map01100 map05206
Pfam Domains
Protein Families
BAH Chromo DNA_methylase

Protein Analysis

1310

Amino Acids

145.69

Weight (kDa)

5.48

Isoelectric Point (pI)

42.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BAH PF01426 198 - 288 1.8e-07 BAH domain
Chromo PF00385 461 - 516 4.1e-14 Chromo (CHRromatin Organisation MOdifier) domain
DNA_methylase PF00145 525 - 645 3e-16 C-5 cytosine-specific DNA methylase
PUNUT PF16913 993 - 1303 1.8e-92 Purine nucleobase transmembrane transport
EamA PF00892 1009 - 1131 6.6e-06 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1838
Acc16I TGCGCA 1 cut(s) 1846
Acc36I ACCTGC 2 cut(s) 1838, 2681
AccB1I GGYRCC 1 cut(s) 3790
AccB7I CCANNNNNTGG 1 cut(s) 1580
AccBSI CCGCTC 2 cut(s) 339, 3840
AccI GTMKAC 5 cut(s) 520, 1076, 1405, 1554, 1861
AccII CGCG 3 cut(s) 50, 729, 2726
AccIII TCCGGA 1 cut(s) 2715
AcoI YGGCCR 3 cut(s) 3, 246, 2785
AcsI RAATTY 7 cut(s) 1538, 1763, 2123, 2132, 2666, 2711, 3166
AcuI CTGAAG 3 cut(s) 80, 1187, 1395
AcvI CACGTG 1 cut(s) 2537
AfaI GTAC 7 cut(s) 228, 238, 314, 850, 1044, 3134, 3700
AfiI CCNNNNNNNGG 8 cut(s) 54, 1580, 1619, 1879, 2069, 3603, 3718, 3839
AflII CTTAAG 1 cut(s) 2924
AflIII ACRYGT 3 cut(s) 577, 2242, 2724
AhlI ACTAGT 2 cut(s) 2746, 2753
AjiI CACGTC 1 cut(s) 1588
AjnI CCWGG 2 cut(s) 1579, 2290
AjuI GAANNNNNNNTTGG 6 cut(s) 759, 791, 1383, 1415, 1670, 1702
AleI CACNNNNGTG 1 cut(s) 1013
AloI GAACNNNNNNTCC 2 cut(s) 1236, 1268
Alw21I GWGCWC 2 cut(s) 2542, 2746
Alw44I GTGCAC 1 cut(s) 2538
AlwNI CAGNNNCTG 2 cut(s) 1373, 2848
Ama87I CYCGRG 3 cut(s) 419, 2852, 3566
Aor13HI TCCGGA 1 cut(s) 2715
AoxI GGCC 9 cut(s) 3, 246, 440, 1081, 1896, 2005, 2762, 2785, 3101
ApaI GGGCCC 1 cut(s) 1900
ApaLI GTGCAC 1 cut(s) 2538
ApeKI GCWGC 8 cut(s) 2602, 2842, 2848, 3212, 3374, 3809, 3885, 3900
ApoI RAATTY 7 cut(s) 1538, 1763, 2123, 2132, 2666, 2711, 3166
Asp700I GAANNNNTTC 2 cut(s) 504, 771
AspA2I CCTAGG 1 cut(s) 3196
AspLEI GCGC 5 cut(s) 15, 298, 729, 1847, 2923
AspS9I GGNCC 7 cut(s) 441, 1607, 1896, 1897, 2374, 2763, 3537
AsuC2I CCSGG 2 cut(s) 2467, 2911
AvaI CYCGRG 3 cut(s) 419, 2852, 3566
AvaII GGWCC 3 cut(s) 1607, 2374, 3537
AvrII CCTAGG 1 cut(s) 3196
AxyI CCTNAGG 2 cut(s) 2064, 2259
BaeGI GKGCMC 2 cut(s) 1900, 2542
BalI TGGCCA 2 cut(s) 5, 2787
BamHI GGATCC 1 cut(s) 2329
BanI GGYRCC 1 cut(s) 3790
BanII GRGCYC 3 cut(s) 1500, 1900, 3489
BarI GAAGNNNNNNTAC 4 cut(s) 210, 242, 1026, 1058
BauI CACGAG 1 cut(s) 975
BbrPI CACGTG 1 cut(s) 2537
BbsI GAAGAC 3 cut(s) 764, 1687, 3744
Bbv12I GWGCWC 2 cut(s) 2542, 2746
BbvI GCAGC 8 cut(s) 2589, 2835, 2854, 3199, 3361, 3821, 3872, 3912
BceAI ACGGC 1 cut(s) 261
BciT130I CCWGG 2 cut(s) 1581, 2292
BclI TGATCA 4 cut(s) 2011, 2203, 2233, 3363
BcnI CCSGG 2 cut(s) 2467, 2911
BcuI ACTAGT 2 cut(s) 2746, 2753
BfmI CTRYAG 2 cut(s) 676, 2517
BfrI CTTAAG 1 cut(s) 2924
BfuAI ACCTGC 2 cut(s) 1838, 2681
BglII AGATCT 1 cut(s) 2056
BlnI CCTAGG 1 cut(s) 3196
BlpI GCTNAGC 1 cut(s) 2144
Bme1390I CCNGG 4 cut(s) 1581, 2292, 2467, 2911
Bme18I GGWCC 3 cut(s) 1607, 2374, 3537
BmeT110I CYCGRG 3 cut(s) 419, 2852, 3566
BmgBI CACGTC 1 cut(s) 1588
BmgT120I GGNCC 7 cut(s) 441, 1607, 1896, 1897, 2374, 2763, 3537
BmiI GGNNCC 9 cut(s) 195, 443, 1497, 1897, 1898, 2331, 2376, 2765, 3792
BmrFI CCNGG 4 cut(s) 1581, 2292, 2467, 2911
BmrI ACTGGG 1 cut(s) 2552
BmuI ACTGGG 1 cut(s) 2552
BpiI GAAGAC 3 cut(s) 764, 1687, 3744
BplI GAGNNNNNCTC 2 cut(s) 1903, 1935
BpmI CTGGAG 2 cut(s) 251, 3575
Bpu1102I GCTNAGC 1 cut(s) 2144
BpuEI CTTGAG 2 cut(s) 530, 1703
BpuMI CCSGG 2 cut(s) 2467, 2911
BsaAI YACGTR 2 cut(s) 2537, 3132
BsaBI GATNNNNATC 1 cut(s) 169
BsaJI CCNNGG 6 cut(s) 420, 1090, 1618, 2291, 3196, 3481
BsaWI WCCGGW 1 cut(s) 2715
BsaXI ACNNNNNCTCC 2 cut(s) 206, 236
Bsc4I CCNNNNNNNGG 8 cut(s) 54, 1580, 1619, 1879, 2069, 3603, 3718, 3839
Bse118I RCCGGY 3 cut(s) 196, 409, 587
Bse1I ACTGG 9 cut(s) 234, 789, 951, 1084, 1558, 2547, 2817, 3324, 3712
Bse21I CCTNAGG 2 cut(s) 2064, 2259
Bse3DI GCAATG 5 cut(s) 2394, 2900, 3006, 3387, 3804
Bse8I GATNNNNATC 1 cut(s) 169
BseAI TCCGGA 1 cut(s) 2715
BseBI CCWGG 2 cut(s) 1581, 2292
BseDI CCNNGG 6 cut(s) 420, 1090, 1618, 2291, 3196, 3481
BseJI GATNNNNATC 1 cut(s) 169
BseLI CCNNNNNNNGG 8 cut(s) 54, 1580, 1619, 1879, 2069, 3603, 3718, 3839
BseMI GCAATG 5 cut(s) 2394, 2900, 3006, 3387, 3804
BseNI ACTGG 9 cut(s) 234, 789, 951, 1084, 1558, 2547, 2817, 3324, 3712
BseRI GAGGAG 6 cut(s) 437, 1346, 1538, 2752, 2888, 3895
BseSI GKGCMC 2 cut(s) 1900, 2542
BseX3I CGGCCG 1 cut(s) 246
BseXI GCAGC 8 cut(s) 2589, 2835, 2854, 3199, 3361, 3821, 3872, 3912
BseYI CCCAGC 2 cut(s) 2766, 3253
Bsh1236I CGCG 3 cut(s) 50, 729, 2726
Bsh1285I CGRYCG 1 cut(s) 249
BshFI GGCC 9 cut(s) 5, 248, 442, 1083, 1898, 2007, 2764, 2787, 3103
BshNI GGYRCC 1 cut(s) 3790
BsiEI CGRYCG 1 cut(s) 249
BsiHKAI GWGCWC 2 cut(s) 2542, 2746
BsiHKCI CYCGRG 3 cut(s) 419, 2852, 3566
BsiSI CCGG 9 cut(s) 32, 89, 197, 410, 433, 588, 2467, 2716, 2911
BslFI GGGAC 4 cut(s) 1257, 2179, 2240, 2360
BslI CCNNNNNNNGG 8 cut(s) 54, 1580, 1619, 1879, 2069, 3603, 3718, 3839
BsmFI GGGAC 4 cut(s) 1257, 2179, 2240, 2360
BsmI GAATGC 1 cut(s) 2230
BsnI GGCC 9 cut(s) 5, 248, 442, 1083, 1898, 2007, 2764, 2787, 3103
BsoBI CYCGRG 3 cut(s) 419, 2852, 3566
Bsp120I GGGCCC 1 cut(s) 1896
Bsp1286I GDGCHC 5 cut(s) 1500, 1900, 2542, 2746, 3489
Bsp13I TCCGGA 1 cut(s) 2715
Bsp1720I GCTNAGC 1 cut(s) 2144
Bsp19I CCATGG 1 cut(s) 3481
BspANI GGCC 9 cut(s) 5, 248, 442, 1083, 1898, 2007, 2764, 2787, 3103
BspEI TCCGGA 1 cut(s) 2715
BspFNI CGCG 3 cut(s) 50, 729, 2726
BspHI TCATGA 1 cut(s) 2236
BspLI GGNNCC 9 cut(s) 195, 443, 1497, 1897, 1898, 2331, 2376, 2765, 3792
BspMI ACCTGC 2 cut(s) 1838, 2681
BspT107I GGYRCC 1 cut(s) 3790
BspTI CTTAAG 1 cut(s) 2924
BsrBI CCGCTC 2 cut(s) 339, 3840
BsrDI GCAATG 5 cut(s) 2394, 2900, 3006, 3387, 3804
BsrFI RCCGGY 3 cut(s) 196, 409, 587
BsrI ACTGG 9 cut(s) 234, 789, 951, 1084, 1558, 2547, 2817, 3324, 3712
BssAI RCCGGY 3 cut(s) 196, 409, 587
BssECI CCNNGG 6 cut(s) 420, 1090, 1618, 2291, 3196, 3481
BssNAI GTATAC 2 cut(s) 1406, 1555
BssSI CACGAG 1 cut(s) 975
BssT1I CCWWGG 4 cut(s) 1090, 1618, 3196, 3481
Bst1107I GTATAC 2 cut(s) 1406, 1555
Bst2BI CACGAG 1 cut(s) 975
Bst2UI CCWGG 2 cut(s) 1581, 2292
Bst6I CTCTTC 5 cut(s) 47, 213, 1323, 1428, 3276
BstAFI CTTAAG 1 cut(s) 2924
BstAPI GCANNNNNTGC 2 cut(s) 2848, 3263
BstBAI YACGTR 2 cut(s) 2537, 3132
BstC8I GCNNGC 6 cut(s) 198, 430, 589, 921, 2907, 2957
BstDSI CCRYGG 1 cut(s) 3481
BstFNI CGCG 3 cut(s) 50, 729, 2726
BstHHI GCGC 5 cut(s) 15, 298, 729, 1847, 2923
BstMCI CGRYCG 1 cut(s) 249
BstMWI GCNNNNNNNGC 7 cut(s) 1103, 2848, 2965, 3017, 3063, 3263, 3644
BstNI CCWGG 2 cut(s) 1581, 2292
BstNSI RCATGY 3 cut(s) 581, 1154, 2909
BstSCI CCNGG 4 cut(s) 1579, 2290, 2465, 2909
BstSFI CTRYAG 2 cut(s) 676, 2517
BstSLI GKGCMC 2 cut(s) 1900, 2542
BstSNI TACGTA 1 cut(s) 3132
BstUI CGCG 3 cut(s) 50, 729, 2726
BstV1I GCAGC 8 cut(s) 2589, 2835, 2854, 3199, 3361, 3821, 3872, 3912
BstV2I GAAGAC 3 cut(s) 764, 1687, 3744
BstX2I RGATCY 8 cut(s) 164, 1138, 1753, 2056, 2329, 3470, 3849, 3919
BstXI CCANNNNNNTGG 1 cut(s) 1015
BstYI RGATCY 8 cut(s) 164, 1138, 1753, 2056, 2329, 3470, 3849, 3919
BstZ17I GTATAC 2 cut(s) 1406, 1555
BstZI CGGCCG 1 cut(s) 246
Bsu36I CCTNAGG 2 cut(s) 2064, 2259
BsuRI GGCC 9 cut(s) 5, 248, 442, 1083, 1898, 2007, 2764, 2787, 3103
BtgI CCRYGG 1 cut(s) 3481
BtrI CACGTC 1 cut(s) 1588
BtsI GCAGTG 2 cut(s) 1928, 2998
BtsIMutI CAGTG 7 cut(s) 674, 958, 1303, 1928, 2540, 2998, 3426
BveI ACCTGC 2 cut(s) 1838, 2681
Cac8I GCNNGC 6 cut(s) 198, 430, 589, 921, 2907, 2957
CaiI CAGNNNCTG 2 cut(s) 1373, 2848
CciI TCATGA 1 cut(s) 2236
CfoI GCGC 5 cut(s) 15, 298, 729, 1847, 2923
Cfr10I RCCGGY 3 cut(s) 196, 409, 587
Cfr13I GGNCC 7 cut(s) 441, 1607, 1896, 1897, 2374, 2763, 3537
CsiI ACCWGGT 1 cut(s) 1579
Csp6I GTAC 7 cut(s) 227, 237, 313, 849, 1043, 3133, 3699
CviQI GTAC 7 cut(s) 227, 237, 313, 849, 1043, 3133, 3699
DraI TTTAAA 2 cut(s) 1261, 3171
EaeI YGGCCR 3 cut(s) 3, 246, 2785
EagI CGGCCG 1 cut(s) 246
Eam1104I CTCTTC 5 cut(s) 47, 213, 1323, 1428, 3276
EarI CTCTTC 5 cut(s) 47, 213, 1323, 1428, 3276
EciI GGCGGA 1 cut(s) 34
EclXI CGGCCG 1 cut(s) 246
Eco105I TACGTA 1 cut(s) 3132
Eco130I CCWWGG 4 cut(s) 1090, 1618, 3196, 3481
Eco24I GRGCYC 3 cut(s) 1500, 1900, 3489
Eco47I GGWCC 3 cut(s) 1607, 2374, 3537
Eco52I CGGCCG 1 cut(s) 246
Eco57I CTGAAG 3 cut(s) 80, 1187, 1395
Eco72I CACGTG 1 cut(s) 2537
Eco81I CCTNAGG 2 cut(s) 2064, 2259
Eco88I CYCGRG 3 cut(s) 419, 2852, 3566
EcoO109I RGGNCCY 3 cut(s) 1896, 1897, 3537
EcoRII CCWGG 2 cut(s) 1579, 2290
EcoT14I CCWWGG 4 cut(s) 1090, 1618, 3196, 3481
EcoT22I ATGCAT 4 cut(s) 2619, 3235, 3241, 3247
EcoT38I GRGCYC 3 cut(s) 1500, 1900, 3489
ErhI CCWWGG 4 cut(s) 1090, 1618, 3196, 3481
FalI AAGNNNNNCTT 2 cut(s) 492, 524
FaqI GGGAC 4 cut(s) 1257, 2179, 2240, 2360
FauI CCCGC 1 cut(s) 1872
FauNDI CATATG 3 cut(s) 1855, 3235, 3241
FbaI TGATCA 4 cut(s) 2011, 2203, 2233, 3363
FblI GTMKAC 5 cut(s) 520, 1076, 1405, 1554, 1861
FriOI GRGCYC 3 cut(s) 1500, 1900, 3489
FspI TGCGCA 1 cut(s) 1846
GlaI GCGC 5 cut(s) 14, 297, 728, 1846, 2922
GsaI CCCAGC 2 cut(s) 2770, 3257
GsuI CTGGAG 2 cut(s) 251, 3575
HaeIII GGCC 9 cut(s) 5, 248, 442, 1083, 1898, 2007, 2764, 2787, 3103
HapII CCGG 9 cut(s) 32, 89, 197, 410, 433, 588, 2467, 2716, 2911
HhaI GCGC 5 cut(s) 15, 298, 729, 1847, 2923
Hin6I GCGC 5 cut(s) 13, 296, 727, 1845, 2921
HinP1I GCGC 5 cut(s) 13, 296, 727, 1845, 2921
HincII GTYRAC 4 cut(s) 521, 898, 985, 2797
HindII GTYRAC 4 cut(s) 521, 898, 985, 2797
HindIII AAGCTT 1 cut(s) 2030
HpaI GTTAAC 1 cut(s) 985
HpaII CCGG 9 cut(s) 32, 89, 197, 410, 433, 588, 2467, 2716, 2911
Hpy188III TCNNGA 7 cut(s) 509, 1645, 2063, 2237, 2333, 2716, 3909
Hpy99I CGWCG 1 cut(s) 522
HpyCH4IV ACGT 7 cut(s) 1408, 1587, 2091, 2242, 2428, 2536, 3131
HpyF10VI GCNNNNNNNGC 7 cut(s) 1103, 2848, 2965, 3017, 3063, 3263, 3644
HpySE526I ACGT 7 cut(s) 1408, 1587, 2091, 2242, 2428, 2536, 3131
HspAI GCGC 5 cut(s) 13, 296, 727, 1845, 2921
Kpn2I TCCGGA 1 cut(s) 2715
KroI GCCGGC 2 cut(s) 196, 587
KroNI GCCGGC 2 cut(s) 198, 589
Ksp22I TGATCA 4 cut(s) 2011, 2203, 2233, 3363
KspAI GTTAAC 1 cut(s) 985
LmnI GCTCC 8 cut(s) 193, 1018, 1475, 1495, 1978, 3556, 3728, 3908
Lsp1109I GCAGC 8 cut(s) 2589, 2835, 2854, 3199, 3361, 3821, 3872, 3912
MabI ACCWGGT 1 cut(s) 1579
MaeII ACGT 7 cut(s) 1408, 1587, 2091, 2242, 2428, 2536, 3131
MbiI CCGCTC 2 cut(s) 339, 3840
MfeI CAATTG 1 cut(s) 1685
MflI RGATCY 8 cut(s) 164, 1138, 1753, 2056, 2329, 3470, 3849, 3919
MhlI GDGCHC 5 cut(s) 1500, 1900, 2542, 2746, 3489
MlsI TGGCCA 2 cut(s) 5, 2787
MluI ACGCGT 1 cut(s) 2724
MluNI TGGCCA 2 cut(s) 5, 2787
MlyI GAGTC 3 cut(s) 230, 460, 2864
MmeI TCCRAC 7 cut(s) 488, 513, 744, 1789, 2019, 2050, 2705
Mox20I TGGCCA 2 cut(s) 5, 2787
Mph1103I ATGCAT 4 cut(s) 2619, 3235, 3241, 3247
MroI TCCGGA 1 cut(s) 2715
MroNI GCCGGC 2 cut(s) 196, 587
MroXI GAANNNNTTC 2 cut(s) 504, 771
MscI TGGCCA 2 cut(s) 5, 2787
MslI CAYNNNNRTG 4 cut(s) 450, 1013, 2187, 2241
Msp20I TGGCCA 2 cut(s) 5, 2787
MspA1I CMGCKG 2 cut(s) 2848, 3812
MspCI CTTAAG 1 cut(s) 2924
MspI CCGG 9 cut(s) 32, 89, 197, 410, 433, 588, 2467, 2716, 2911
MspR9I CCNGG 4 cut(s) 1581, 2292, 2467, 2911
MunI CAATTG 1 cut(s) 1685
Mva1269I GAATGC 1 cut(s) 2230
MvaI CCWGG 2 cut(s) 1581, 2292
MvnI CGCG 3 cut(s) 50, 729, 2726
MwoI GCNNNNNNNGC 7 cut(s) 1103, 2848, 2965, 3017, 3063, 3263, 3644
NaeI GCCGGC 2 cut(s) 198, 589
NciI CCSGG 2 cut(s) 2467, 2911
NcoI CCATGG 1 cut(s) 3481
NdeI CATATG 3 cut(s) 1855, 3235, 3241
NgoMIV GCCGGC 2 cut(s) 196, 587
NlaIV GGNNCC 9 cut(s) 195, 443, 1497, 1897, 1898, 2331, 2376, 2765, 3792
NmeAIII GCCGAG 2 cut(s) 274, 1082
NmuCI GTSAC 7 cut(s) 130, 812, 860, 1583, 2087, 2749, 3424
NsbI TGCGCA 1 cut(s) 1846
NsiI ATGCAT 4 cut(s) 2619, 3235, 3241, 3247
NspI RCATGY 3 cut(s) 581, 1154, 2909
OliI CACNNNNGTG 1 cut(s) 1013
PaeI GCATGC 1 cut(s) 2909
PaeR7I CTCGAG 1 cut(s) 2852
PagI TCATGA 1 cut(s) 2236
PaqCI CACCTGC 1 cut(s) 1838
PciI ACATGT 1 cut(s) 577
PcsI WCGNNNNNNNCGW 1 cut(s) 2248
PctI GAATGC 1 cut(s) 2230
PdiI GCCGGC 2 cut(s) 198, 589
PdmI GAANNNNTTC 2 cut(s) 504, 771
PflFI GACNNNGTC 1 cut(s) 3422
PflMI CCANNNNNTGG 1 cut(s) 1580
PleI GAGTC 3 cut(s) 229, 460, 2863
PmaCI CACGTG 1 cut(s) 2537
PmlI CACGTG 1 cut(s) 2537
PpsI GAGTC 3 cut(s) 229, 460, 2863
Ppu21I YACGTR 2 cut(s) 2537, 3132
PpuMI RGGWCCY 1 cut(s) 3537
PscI ACATGT 1 cut(s) 577
Psp5II RGGWCCY 1 cut(s) 3537
Psp6I CCWGG 2 cut(s) 1579, 2290
PspCI CACGTG 1 cut(s) 2537
PspFI CCCAGC 2 cut(s) 2766, 3253
PspGI CCWGG 2 cut(s) 1579, 2290
PspN4I GGNNCC 9 cut(s) 195, 443, 1497, 1897, 1898, 2331, 2376, 2765, 3792
PspOMI GGGCCC 1 cut(s) 1896
PspPI GGNCC 7 cut(s) 441, 1607, 1896, 1897, 2374, 2763, 3537
PspPPI RGGWCCY 1 cut(s) 3537
PspXI VCTCGAGB 1 cut(s) 2852
PstNI CAGNNNCTG 2 cut(s) 1373, 2848
PsuI RGATCY 8 cut(s) 164, 1138, 1753, 2056, 2329, 3470, 3849, 3919
PsyI GACNNNGTC 1 cut(s) 3422
PvuII CAGCTG 1 cut(s) 3812
RsaI GTAC 7 cut(s) 228, 238, 314, 850, 1044, 3134, 3700
RsaNI GTAC 7 cut(s) 227, 237, 313, 849, 1043, 3133, 3699
RseI CAYNNNNRTG 4 cut(s) 450, 1013, 2187, 2241
SalI GTCGAC 1 cut(s) 519
Sau96I GGNCC 7 cut(s) 441, 1607, 1896, 1897, 2374, 2763, 3537
SchI GAGTC 3 cut(s) 230, 460, 2864
ScrFI CCNGG 4 cut(s) 1581, 2292, 2467, 2911
SduI GDGCHC 5 cut(s) 1500, 1900, 2542, 2746, 3489
SexAI ACCWGGT 1 cut(s) 1579
SfcI CTRYAG 2 cut(s) 676, 2517
Sfr274I CTCGAG 1 cut(s) 2852
SinI GGWCC 3 cut(s) 1607, 2374, 3537
SlaI CTCGAG 1 cut(s) 2852
SmiMI CAYNNNNRTG 4 cut(s) 450, 1013, 2187, 2241
SmlI CTYRAG 4 cut(s) 509, 1718, 2852, 2924
SmoI CTYRAG 4 cut(s) 509, 1718, 2852, 2924
SnaBI TACGTA 1 cut(s) 3132
SpeI ACTAGT 2 cut(s) 2746, 2753
SphI GCATGC 1 cut(s) 2909
StyD4I CCNGG 4 cut(s) 1579, 2290, 2465, 2909
StyI CCWWGG 4 cut(s) 1090, 1618, 3196, 3481
TaiI ACGT 7 cut(s) 1411, 1590, 2094, 2245, 2431, 2539, 3134
TaqI TCGA 8 cut(s) 20, 105, 305, 520, 711, 2708, 2853, 3657
TatI WGTACW 3 cut(s) 312, 1042, 3698
TauI GCSGC 6 cut(s) 50, 203, 2848, 2999, 3060, 3843
TscAI CASTG 7 cut(s) 674, 958, 1303, 1935, 2547, 2998, 3433
TseFI GTSAC 7 cut(s) 130, 812, 860, 1583, 2087, 2749, 3424
TseI GCWGC 8 cut(s) 2602, 2842, 2848, 3212, 3374, 3809, 3885, 3900
Tsp45I GTSAC 7 cut(s) 130, 812, 860, 1583, 2087, 2749, 3424
TspGWI ACGGA 2 cut(s) 1862, 2643
TspRI CASTG 7 cut(s) 674, 958, 1303, 1935, 2547, 2998, 3433
Tth111I GACNNNGTC 1 cut(s) 3422
Van91I CCANNNNNTGG 1 cut(s) 1580
Vha464I CTTAAG 1 cut(s) 2924
VneI GTGCAC 1 cut(s) 2538
VpaK11BI GGWCC 3 cut(s) 1607, 2374, 3537
XapI RAATTY 7 cut(s) 1538, 1763, 2123, 2132, 2666, 2711, 3166
XceI RCATGY 3 cut(s) 581, 1154, 2909
XcmI CCANNNNNNNNNTGG 2 cut(s) 2967, 3391
XhoI CTCGAG 1 cut(s) 2852
XmaJI CCTAGG 1 cut(s) 3196
XmiI GTMKAC 5 cut(s) 520, 1076, 1405, 1554, 1861
XmnI GAANNNNTTC 2 cut(s) 504, 771
Zsp2I ATGCAT 4 cut(s) 2619, 3235, 3241, 3247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.