Rorug04G0349300

DNA (cytosine-5)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0001701
Physical Location & Seq
Reverse (-)
52172127 .. 52175020
2894 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0349300.1

Sequence Viewer

Length: 975 bp
ATGGCGGGCACTCTGGCTTTGATACCAAGCAATCCGCCGGTGACGTCATCGTCTCTGAGCTCCATCCGAGAGCGGTGGGAGGTCGAGTTCGCTCGCTTCTTCGGCTACCCTCCGCTCACCTCCACGTGTCCAGATCTCGTCCCTCTGCCTATGAAGGTCAGGAATCGACGGCCGGTCGGTAACTGGATCTCCGCCGCCTCCGCCGCCATGCTTCAACTCATTTATGACCACTCCAGCTCCGACGTCATCCTCACCGTCTGCTCCAGCGGCAAGATCATGGAGGAGCACTACGTGTCGAAGCTACTCTTTGCCTGGCCTCAGGTTGCATGCATGTCTGGATATCCTGCCAGGGGCAGTAGAGCCATCTTTGCCAGCTACAGAGATTCTTTGGGCCAGATCCAGAAACTTGCTTTTCGATTTTTGTCAGTCAATGAAGCAGAGAAGTTCATAACTCTTATGAAGGAGATTTTCAAGGAAGGGAGGGATATTCAACCTCTTAATACTGACTATGGATCTGAAATTTCATCACAATCTGAGTTCATGTCTTCTAATAGACCTCCAAACAGAACTTCCAAGGACTTGGTTGTCATGACTCCTGTTCACAGTCATTCTCCTCAAATATCACCAAGCTTAATGACGGCTTATACTCCTCAAATGTCACCAAGCTTAATGAAGGATTATACACCTCAAATGTCACCAACCTGGAAACATGAAGCAGCTCAACACAGACAAACTCGGCACATGTCACCTATTCAGAACTTTCAGAGCAACTTTGCTGCCTTTCCTCCTAGTTTCACCTCATTAGTGTCCAACTGTCAACCTCTGGTTGAGCAAGCTCCAGCACAATCAACTGCATCAAAGGAAGTCGATCTCAAGGCCCTAATTGCGAGATGTATGGAAGACTCTTCCTTCCAAGATATATTGGGCCAAGTTGAGACAGTTATGGGCGAAATGGGGGCTGATTTGAAGCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko01100 ko05206 map00270 map01100 map05206
Pfam Domains
Protein Families
BAH Chromo DNA_methylase

Protein Analysis

324

Amino Acids

36.03

Weight (kDa)

7.05

Isoelectric Point (pI)

59.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH_PHS1 PF25349 24 - 166 9.1e-61 Poor homologous synapsis protein PH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 49, 584
AatII GACGTC 2 cut(s) 47, 246
AccBSI CCGCTC 2 cut(s) 73, 115
AciI CCGC 9 cut(s) 5, 35, 73, 113, 192, 195, 201, 204, 267
AclWI GGATC 3 cut(s) 194, 391, 520
AcoI YGGCCR 1 cut(s) 170
AcsI RAATTY 1 cut(s) 519
AcvI CACGTG 1 cut(s) 126
AcyI GRCGYC 2 cut(s) 44, 243
AdeI CACNNNGTG 1 cut(s) 292
AfiI CCNNNNNNNGG 1 cut(s) 350
AflIII ACRYGT 3 cut(s) 125, 291, 741
AgsI TTSAA 4 cut(s) 215, 472, 491, 967
AhdI GACNNNNNGTC 1 cut(s) 173
AjnI CCWGG 3 cut(s) 311, 347, 701
AjuI GAANNNNNNNTTGG 2 cut(s) 553, 585
AloI GAACNNNNNNTCC 2 cut(s) 71, 103
AluBI AGCT 9 cut(s) 60, 237, 301, 375, 630, 666, 719, 836, 970
AluI AGCT 9 cut(s) 60, 237, 301, 375, 630, 666, 719, 836, 970
Alw21I GWGCWC 2 cut(s) 62, 288
Alw26I GTCTC 2 cut(s) 57, 929
AlwI GGATC 3 cut(s) 194, 391, 520
AoxI GGCC 5 cut(s) 170, 314, 391, 876, 925
ApeKI GCWGC 2 cut(s) 716, 776
ApoI RAATTY 1 cut(s) 519
AspS9I GGNCC 3 cut(s) 391, 877, 925
AsuHPI GGTGA 8 cut(s) 52, 109, 244, 615, 651, 687, 738, 787
AxyI CCTNAGG 1 cut(s) 318
BaeGI GKGCMC 1 cut(s) 11
BanII GRGCYC 1 cut(s) 62
BbrPI CACGTG 1 cut(s) 126
BbsI GAAGAC 2 cut(s) 537, 906
Bbv12I GWGCWC 2 cut(s) 62, 288
BbvI GCAGC 2 cut(s) 728, 763
BccI CCATC 2 cut(s) 71, 371
BceAI ACGGC 2 cut(s) 185, 654
BciT130I CCWGG 3 cut(s) 313, 349, 703
BcoDI GTCTC 2 cut(s) 57, 929
BfaI CTAG 1 cut(s) 789
BfmI CTRYAG 2 cut(s) 376, 971
BglII AGATCT 1 cut(s) 133
BisI GCNGC 5 cut(s) 195, 204, 268, 717, 777
BlsI GCNGC 5 cut(s) 196, 205, 269, 718, 778
Bme1390I CCNGG 3 cut(s) 313, 349, 703
BmeRI GACNNNNNGTC 1 cut(s) 173
BmgT120I GGNCC 3 cut(s) 391, 877, 925
BmrFI CCNGG 3 cut(s) 313, 349, 703
BmsI GCATC 1 cut(s) 863
BpiI GAAGAC 2 cut(s) 537, 906
BpmI CTGGAG 3 cut(s) 217, 247, 822
BpuEI CTTGAG 1 cut(s) 857
BsaAI YACGTR 2 cut(s) 126, 292
BsaHI GRCGYC 2 cut(s) 44, 243
BsaJI CCNNGG 2 cut(s) 348, 573
BsaXI ACNNNNNCTCC 6 cut(s) 71, 101, 173, 203, 221, 251
Bsc4I CCNNNNNNNGG 1 cut(s) 350
Bse118I RCCGGY 2 cut(s) 37, 172
Bse1I ACTGG 1 cut(s) 188
Bse21I CCTNAGG 1 cut(s) 318
BseBI CCWGG 3 cut(s) 313, 349, 703
BseDI CCNNGG 2 cut(s) 348, 573
BseGI GGATG 2 cut(s) 63, 246
BseLI CCNNNNNNNGG 1 cut(s) 350
BseMII CTCAG 3 cut(s) 47, 332, 525
BseNI ACTGG 1 cut(s) 188
BseRI GAGGAG 3 cut(s) 296, 603, 639
BseSI GKGCMC 1 cut(s) 11
BseX3I CGGCCG 1 cut(s) 170
BseXI GCAGC 2 cut(s) 728, 763
Bsh1285I CGRYCG 2 cut(s) 173, 177
BshFI GGCC 5 cut(s) 172, 316, 393, 878, 927
BsiEI CGRYCG 2 cut(s) 173, 177
BsiHKAI GWGCWC 2 cut(s) 62, 288
BsiSI CCGG 2 cut(s) 38, 173
BslFI GGGAC 1 cut(s) 125
BslI CCNNNNNNNGG 1 cut(s) 350
BsmAI GTCTC 2 cut(s) 57, 929
BsmBI CGTCTC 1 cut(s) 57
BsmFI GGGAC 1 cut(s) 125
BsnI GGCC 5 cut(s) 172, 316, 393, 878, 927
Bsp1286I GDGCHC 3 cut(s) 11, 62, 288
Bsp143I GATC 6 cut(s) 133, 186, 273, 396, 512, 868
BspACI CCGC 9 cut(s) 5, 35, 73, 113, 192, 195, 201, 204, 267
BspANI GGCC 5 cut(s) 172, 316, 393, 878, 927
BspCNI CTCAG 3 cut(s) 48, 331, 526
BspHI TCATGA 1 cut(s) 588
BspPI GGATC 3 cut(s) 194, 391, 520
BsrBI CCGCTC 2 cut(s) 73, 115
BsrFI RCCGGY 2 cut(s) 37, 172
BsrI ACTGG 1 cut(s) 188
BssAI RCCGGY 2 cut(s) 37, 172
BssECI CCNNGG 2 cut(s) 348, 573
BssMI GATC 6 cut(s) 133, 186, 273, 396, 512, 868
BssNI GRCGYC 2 cut(s) 44, 243
BssT1I CCWWGG 1 cut(s) 573
Bst2UI CCWGG 3 cut(s) 313, 349, 703
Bst4CI ACNGT 4 cut(s) 256, 605, 815, 940
Bst6I CTCTTC 1 cut(s) 910
BstACI GRCGYC 2 cut(s) 44, 243
BstBAI YACGTR 2 cut(s) 126, 292
BstC8I GCNNGC 5 cut(s) 7, 94, 328, 373, 834
BstDEI CTNAG 3 cut(s) 56, 318, 534
BstF5I GGATG 2 cut(s) 63, 246
BstKTI GATC 6 cut(s) 136, 189, 276, 399, 515, 871
BstMAI GTCTC 2 cut(s) 57, 929
BstMBI GATC 6 cut(s) 133, 186, 273, 396, 512, 868
BstMCI CGRYCG 2 cut(s) 173, 177
BstMWI GCNNNNNNNGC 6 cut(s) 102, 200, 203, 267, 368, 884
BstNI CCWGG 3 cut(s) 313, 349, 703
BstNSI RCATGY 3 cut(s) 330, 334, 745
BstSCI CCNGG 3 cut(s) 311, 347, 701
BstSFI CTRYAG 2 cut(s) 376, 971
BstSLI GKGCMC 1 cut(s) 11
BstV1I GCAGC 2 cut(s) 728, 763
BstV2I GAAGAC 2 cut(s) 537, 906
BstX2I RGATCY 4 cut(s) 133, 186, 396, 512
BstXI CCANNNNNNTGG 1 cut(s) 580
BstYI RGATCY 4 cut(s) 133, 186, 396, 512
BstZI CGGCCG 1 cut(s) 170
Bsu36I CCTNAGG 1 cut(s) 318
BsuRI GGCC 5 cut(s) 172, 316, 393, 878, 927
BtsCI GGATG 2 cut(s) 63, 246
Cac8I GCNNGC 5 cut(s) 7, 94, 328, 373, 834
CciI TCATGA 1 cut(s) 588
Cfr10I RCCGGY 2 cut(s) 37, 172
Cfr13I GGNCC 3 cut(s) 391, 877, 925
CviAII CATG 8 cut(s) 208, 277, 327, 331, 541, 589, 710, 742
DdeI CTNAG 3 cut(s) 56, 318, 534
DpnI GATC 6 cut(s) 135, 188, 275, 398, 514, 870
DpnII GATC 6 cut(s) 133, 186, 273, 396, 512, 868
DraIII CACNNNGTG 1 cut(s) 292
DrdI GACNNNNNNGTC 2 cut(s) 49, 584
DriI GACNNNNNGTC 1 cut(s) 173
DseDI GACNNNNNNGTC 2 cut(s) 49, 584
EaeI YGGCCR 1 cut(s) 170
EagI CGGCCG 1 cut(s) 170
Eam1104I CTCTTC 1 cut(s) 910
Eam1105I GACNNNNNGTC 1 cut(s) 173
EarI CTCTTC 1 cut(s) 910
EciI GGCGGA 3 cut(s) 24, 181, 190
Ecl136II GAGCTC 1 cut(s) 60
EclXI CGGCCG 1 cut(s) 170
Eco130I CCWWGG 1 cut(s) 573
Eco24I GRGCYC 1 cut(s) 62
Eco32I GATATC 1 cut(s) 341
Eco52I CGGCCG 1 cut(s) 170
Eco53kI GAGCTC 1 cut(s) 60
Eco72I CACGTG 1 cut(s) 126
Eco81I CCTNAGG 1 cut(s) 318
EcoICRI GAGCTC 1 cut(s) 60
EcoO109I RGGNCCY 1 cut(s) 877
EcoRII CCWGG 3 cut(s) 311, 347, 701
EcoRV GATATC 1 cut(s) 341
EcoT14I CCWWGG 1 cut(s) 573
EcoT22I ATGCAT 1 cut(s) 332
EcoT38I GRGCYC 1 cut(s) 62
ErhI CCWWGG 1 cut(s) 573
Esp3I CGTCTC 1 cut(s) 57
FaeI CATG 8 cut(s) 211, 280, 330, 334, 544, 592, 713, 745
FalI AAGNNNNNCTT 2 cut(s) 290, 322
FaqI GGGAC 1 cut(s) 125
FatI CATG 8 cut(s) 207, 276, 326, 330, 540, 588, 709, 741
Fnu4HI GCNGC 5 cut(s) 195, 204, 268, 717, 777
FokI GGATG 2 cut(s) 50, 233
FriOI GRGCYC 1 cut(s) 62
Fsp4HI GCNGC 5 cut(s) 195, 204, 268, 717, 777
FspBI CTAG 1 cut(s) 789
GluI GCNGC 5 cut(s) 195, 204, 268, 717, 777
GsuI CTGGAG 3 cut(s) 217, 247, 822
HaeIII GGCC 5 cut(s) 172, 316, 393, 878, 927
HapII CCGG 2 cut(s) 38, 173
Hin1I GRCGYC 2 cut(s) 44, 243
Hin1II CATG 8 cut(s) 211, 280, 330, 334, 544, 592, 713, 745
HincII GTYRAC 1 cut(s) 818
HindII GTYRAC 1 cut(s) 818
HindIII AAGCTT 2 cut(s) 628, 664
HinfI GANTC 4 cut(s) 163, 383, 592, 902
HpaII CCGG 2 cut(s) 38, 173
HphI GGTGA 8 cut(s) 52, 109, 244, 615, 651, 687, 738, 787
Hpy166II GTNNAC 2 cut(s) 601, 818
Hpy188I TCNGA 7 cut(s) 57, 68, 241, 517, 535, 756, 765
Hpy188III TCNNGA 5 cut(s) 131, 160, 336, 400, 589
Hpy8I GTNNAC 2 cut(s) 601, 818
Hpy99I CGWCG 2 cut(s) 171, 245
HpyAV CCTTC 5 cut(s) 148, 454, 470, 667, 919
HpyCH4III ACNGT 4 cut(s) 256, 605, 815, 940
HpyCH4IV ACGT 4 cut(s) 44, 125, 243, 291
HpyCH4V TGCA 3 cut(s) 326, 330, 854
HpyF10VI GCNNNNNNNGC 6 cut(s) 102, 200, 203, 267, 368, 884
HpyF3I CTNAG 3 cut(s) 56, 318, 534
HpySE526I ACGT 4 cut(s) 44, 125, 243, 291
Hsp92I GRCGYC 2 cut(s) 44, 243
Hsp92II CATG 8 cut(s) 211, 280, 330, 334, 544, 592, 713, 745
Kzo9I GATC 6 cut(s) 133, 186, 273, 396, 512, 868
LmnI GCTCC 5 cut(s) 65, 242, 266, 283, 841
Lsp1109I GCAGC 2 cut(s) 728, 763
LweI GCATC 1 cut(s) 863
MaeI CTAG 1 cut(s) 789
MaeII ACGT 4 cut(s) 44, 125, 243, 291
MaeIII GTNAC 5 cut(s) 40, 179, 657, 693, 744
MalI GATC 6 cut(s) 135, 188, 275, 398, 514, 870
MbiI CCGCTC 2 cut(s) 73, 115
MboI GATC 6 cut(s) 133, 186, 273, 396, 512, 868
MboII GAAGA 4 cut(s) 91, 537, 897, 911
MflI RGATCY 4 cut(s) 133, 186, 396, 512
MhlI GDGCHC 3 cut(s) 11, 62, 288
MluCI AATT 2 cut(s) 519, 882
MlyI GAGTC 2 cut(s) 586, 896
MmeI TCCRAC 2 cut(s) 264, 834
Mph1103I ATGCAT 1 cut(s) 332
MseI TTAA 3 cut(s) 498, 632, 668
MspA1I CMGCKG 1 cut(s) 267
MspI CCGG 2 cut(s) 38, 173
MspR9I CCNGG 3 cut(s) 313, 349, 703
MvaI CCWGG 3 cut(s) 313, 349, 703
MwoI GCNNNNNNNGC 6 cut(s) 102, 200, 203, 267, 368, 884
NdeII GATC 6 cut(s) 133, 186, 273, 396, 512, 868
NlaIII CATG 8 cut(s) 211, 280, 330, 334, 544, 592, 713, 745
NmeAIII GCCGAG 1 cut(s) 715
NmuCI GTSAC 4 cut(s) 40, 657, 693, 744
NsiI ATGCAT 1 cut(s) 332
NspI RCATGY 3 cut(s) 330, 334, 745
PaeI GCATGC 1 cut(s) 330
PagI TCATGA 1 cut(s) 588
PciI ACATGT 1 cut(s) 741
PfeI GAWTC 2 cut(s) 163, 383
PkrI GCNGC 5 cut(s) 196, 205, 269, 718, 778
PleI GAGTC 2 cut(s) 586, 896
PmaCI CACGTG 1 cut(s) 126
PmlI CACGTG 1 cut(s) 126
PpsI GAGTC 2 cut(s) 586, 896
Ppu21I YACGTR 2 cut(s) 126, 292
PscI ACATGT 1 cut(s) 741
Psp124BI GAGCTC 1 cut(s) 62
Psp6I CCWGG 3 cut(s) 311, 347, 701
PspCI CACGTG 1 cut(s) 126
PspGI CCWGG 3 cut(s) 311, 347, 701
PspPI GGNCC 3 cut(s) 391, 877, 925
PsuI RGATCY 4 cut(s) 133, 186, 396, 512
SacI GAGCTC 1 cut(s) 62
SaqAI TTAA 3 cut(s) 498, 632, 668
SatI GCNGC 5 cut(s) 195, 204, 268, 717, 777
Sau3AI GATC 6 cut(s) 133, 186, 273, 396, 512, 868
Sau96I GGNCC 3 cut(s) 391, 877, 925
SchI GAGTC 2 cut(s) 586, 896
ScrFI CCNGG 3 cut(s) 313, 349, 703
SduI GDGCHC 3 cut(s) 11, 62, 288
SfaNI GCATC 1 cut(s) 863
SfcI CTRYAG 2 cut(s) 376, 971
SgrAI CRCCGGYG 1 cut(s) 37
SmlI CTYRAG 1 cut(s) 872
SmoI CTYRAG 1 cut(s) 872
SphI GCATGC 1 cut(s) 330
Sse9I AATT 2 cut(s) 519, 882
SsiI CCGC 9 cut(s) 5, 35, 73, 113, 192, 195, 201, 204, 267
SspMI CTAG 1 cut(s) 789
SstI GAGCTC 1 cut(s) 62
StyD4I CCNGG 3 cut(s) 311, 347, 701
StyI CCWWGG 1 cut(s) 573
TaaI ACNGT 4 cut(s) 256, 605, 815, 940
TaiI ACGT 4 cut(s) 47, 128, 246, 294
TaqI TCGA 5 cut(s) 84, 166, 296, 415, 867
TasI AATT 2 cut(s) 519, 882
TauI GCSGC 3 cut(s) 197, 206, 270
TfiI GAWTC 2 cut(s) 163, 383
Tru1I TTAA 3 cut(s) 498, 632, 668
Tru9I TTAA 3 cut(s) 498, 632, 668
TseFI GTSAC 4 cut(s) 40, 657, 693, 744
TseI GCWGC 2 cut(s) 716, 776
Tsp45I GTSAC 4 cut(s) 40, 657, 693, 744
TspDTI ATGAA 8 cut(s) 167, 436, 447, 473, 513, 529, 686, 726
XapI RAATTY 1 cut(s) 519
XceI RCATGY 3 cut(s) 330, 334, 745
XspI CTAG 1 cut(s) 789
ZraI GACGTC 2 cut(s) 45, 244
Zsp2I ATGCAT 1 cut(s) 332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.