Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0001408

Physical Location & Seq

Genomic Coordinates Reverse (-)

7681200 .. 7687190

Gene Structure 5991 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1109500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4368 bp

ATGGATGTCTCACTGGAGATTTCGAATGTAGCTTTTTTCATAGTGTTATTAACGTGGGTTTTGCTTGATATTATGAGAGGAAGAAAAAATGGTAGCCAAACAGGTTTGAGATATAGAGCGGAAGACAGAGGGTGCAAATTTCCTGATTTGGTCACCTTTTCAGCAAATGCTTTAATCTCAAGCTTTTACCTTGGTTTTGGGATTTATGAGTATTGGGGTGGTAGAATTGTAAGCTGTAAATCCATCTTCTCAGGCATGACTTGGGTTTTGGCCACAGTAGTTACAGGTTACTCAATGATTTACAGAGCTCACAGTGAGCCAAAAAGGTGGCCTTGGGTCCTCAGTTTCTGGTGGATCTTTTCCTGCATTTTTTACTCACTTTCTGTGTGCTTTTACCTCATTGCTCACTTCAAATTCTACAAATTTCCAGAGATTTTCCCAAAGGCTAGTTTAGTCGATTTCGCTTCGTTTCCTTTGTCAATACTGCTTTGTTTCAATGCTTTTAGTTACGTCCAGAAAAACAATGACCTTAAACACCCATTACTTGAGAAGGAAGATGAAATTCCCTCCCAAGAGAGTGACACTTACACCAATGCTGGAATTTGGAGTAAGGTTACATTTCAATGGCTGAACCCGCTTTTCAAACGGGGTCGAACCCAAAAGCTGGAATTGCCTCATATACCATCTATTCCTCCCTCAGAAAGAGCAGCATACGCTTCCTCTTTGCTCGACGAATCACTGAGCAAACAGAAGATGGAAGACTCTTCGTTGCCAAAATCCATAATGCTGGCTGTGCGAAGATCATTAGCTGTAAATGCTGTTTTTGCCGGAGTCAATACGGCTGCATCATATATAGGTCCTTTTCTGATTACAAATTTTGTAAATTATTTACTCGAAAAACAAGATGATTCAGGCAACCGCCATGGACTGATTCTTGCGTTAGTATTTTTCGTTGCTAAGACATTGGAATCATTGAGTCAAAGACAGTGGTATTTTGGTGCTCACTTAATTGGTGTACGAGTAAGAGCAGCTCTCACTGTGTTGATTTACAAGAAATCTATTTCCATCAAGTATTCGGGTCCAAGCAATGGTAAAATTATTAATTTGATCAATGTGGATGTTGAAAGAATTGGAGACTTCTGCTGGTACGTTCATGGCATCTGGTTGCTCCCGCTTCAGGTATTTTTGGCCTTGGCTATCCTTTACAGGAATCTTGGTGCAGCCCCCTCTGCTGCTGCTCTTCTTTCCACTGTTTTGGTCATGGTATGCAACACACCGTTGGCCAATAGGCAAAAAAGGCTTCACTCCAAGATCATGGAAGCGAAGGATTCGAGAATCAAAATAACATCGGAGATTCTGAAGAGCATGAGAGTTTTAAAGCTGCATTCATGGGAGTCCACATTCTTGAAGAAGCTGCTTCAGCTCAGGGAAACAGAGAGGCATTGGCTGAAGAGATACCTTTATACATGCTCGGCGGTTGCCTTTCTCTTCTGGGCTTCACCAACTCTAGTTTCAGTTACCACTTTTGGTGTTTGCATTATACTAAACACACCTTTGACAGTAGGTACTGTATTGTCCGCTTTAGCTACTTTCAGAGTTCTACAAGAGCCTATCTACAACCTGCCAGAGCTCATTTCCATGATTACACAGACAAAGGTATCGATTGATCGTATCCAAGAATTCATCAAAGAAGATCAAATGAAGCTGATTCCTTGCCATCATTCAAAAATATCCAATGTCATGATTGTTATCGAGACAGGAGAGTATGAGTGGAAAACAAGCAATCAAAACTTAAAGAAACCTACAATCAGAATTACAGAGAAAATAAAAATACCGCAGGGATACAAGGTTGCTGTTTGTGGCTCAGTTGGTTCTGGCAAGTCAAGCCTACTATTGAGTATACTTGGTGAGATTCCCAAAATTTCTGGGCCAGGAGCAAAAGTTTATGGCACAAAAGCTTATGTTCCACAAAGTGCTTGGATTCAGACTGGAACGATAAGGGAGAATGTATTGTTTGGCAGGAAAATGAAAAGGGGATTCTATGAGGATGTTTTGGAAATTTGTGCTTTGGACCAGGATGTCAAGATGTGGGCAGATGGAGATTTGACGGTAGTGGGAGAGAGAGGGATGAACCTAAGTGGAGGAGAAAAGCAAAGAATTCAACTCGCACGAGCTGTGTACAGCGATTCAGATGTTTATATTTTGGACGATCCTTTCAGTGCTGTTGATGCACATACCGGAACACACTTGTTTAAGAAATGTCTCTTGCAACACTTGTCCCAGAAGACTGTTATCTATGCCACCCACCAATTAGAATTTTTAGAGGCAGCAGACCTTGTCTTGGTTATTAAGGATGGTCAAATTTCTCAGTCTGGAAAATATGAAGATTTGATTGTGGATCCCAACAGTGAACTTGTTAGACAAATGTCTGCACATAAAAAATCATTTGAACAAGTTAACACTTGCCAACAAGATGATTCCTTTAACAGAAAATCTCATCAAGTTAATCTCATAGAGGTTTTAGATGAAGAAGAAAAGCCTTTCAACAACGGCAAGCTTTCAGAAAAAAGTCATGAAGAGGAAGCGGTAACTGGTCGAGTAAAATGGAATGTTTACTCAACATTTGTCACTTCTGCTTATAGAGGTGCTCTTGTCCCGGTCATCCTACTCTGTCAAGTCCTCTTCCAGGGACTACAAATGGGAAGCAATTACTGGATTGCTTGGGCAGCGGAGAAAGAATCCAGGGTCAGCAAACAGCGGTTGATTTGGGTATTTGCTCTGTTGTCTGGTGGGAGTTCCATCTTCATATTGGGAAGGGCAGTTTTTCTGGCAACTATTGCTATTCAGACTTCTCAGCGTCTCTTCCTTGGAATGATTACTTCAGTTTTCCGAGCACCAATTTCATTTTTTGACTCCACACCTTCTAGTCGAATCCTCAATAGGTCTTCTTCGGATCAAAGCACAGTAGACATTGATATTCCATATAGATTAGCTGGATTGGTATTTGCCCTCATCCAGCTAATAAGCATCATCATCCTTATGTCTCAAGTGGCCTGGCAAGTCTTCATTCTTTTCCTTCTTGTTCTTGCACTCTCCGGCTGGTATCAAGCTTATTACATTACCACAGCTAGAGAACTAGCCAGGATGGTTGGAATTCGAAAAGCTCCAATTCTTCATCACTTTTCAGAATCGATATCAGGGGCAGGAACAATTCGGTGTTTCAGTCAAGAAGGCCGCTTCCTGATGAAAGTAATGACCTTGATTGATGATTATTCTCGCGTAGCCTTTCACAATTATGGCACGATGGAGTGGCTATCTGTTCGGACGAACTTTCTGTTTAACCTTGTCTACTTCCTACTTCTGATCATCTTGGTGAGCTTGCCTAGGTCTGCCATTGACCCTAGTTTGGCGGGATTGGCAGCTACCTATGGTTTGAACCTGAATGTCCTTCAGGCTTGGGTAATATGGAATATGTGCAATGTAGAAAACAAAATGATTTCTGTCGAGCGGATTCTTCAATTCACAAATATACCAAGTGAGGCACCGCTTGTGATTGAAGATTGTAGACCAGCGCCTCAATGGCCTATGGCCGGAAGAATTGAACTTGAAAACCTTCATGTGCAATATAATCCAGCTCTCCCAACCATTCTCAAGGGGATCACTTGCACCTTCCCTGGAATGAAAAAAATAGGAATTGTGGGCAGGACTGGGAGTGGAAAATCCACTCTGATCCAAGCTCTCTTCAGAGTAGTAGAACCCTCGGGAGGGCGAATTCTTATTGATGGTGTCGATATATCTAAAATAGGTCTGCAGGACTTGAGGTCAAGGTTGAGCATAATTCCACAAGACCCGACATTATTTCAGGGAACCGTGAGGACCAATCTGGATCCTTTGCAACAGCATTCTGATCAAGAGATATGGGAGGTTATCAATCAATGCGGACTGACGGAGATTGTTAGGCAGGACCAAAGGCTTCTTGATGCACCTGTTGCTGAAGACGGAGAAAACTGGAGTGTTGGACAGAGGCAGCTCGTTTGCCTCGCTCGAGTGCTCTTGAAGAAAAAGAAAATTCTTGTGTTGGATGAGGCTACAGCTTCTATTGATACAGCAACAGATATTGTAATCCAGGAGACCATAAGGAAAGAAACGAGTGGATGCACCGTGGTCACTGTGGCTCACAGGATTCCTACTGTTATTGACAACGACTTGGTTCTAGTTCTTGACGAAGGCAAGGTTTTGGAGTACGATTCTCCAGCTCGGTTACTTGAGGATAGTTCTTCGGCATTCTCCAAGTTGGTGGCAGAATTCTTGGGGAGATCTTCCATGAGCAGCTGCTACAGAGATCCAAGTAGAGGCGCATCTTTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0003674 GO:0003824 GO:0005215 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005911 GO:0006810 GO:0008150 GO:0009506 GO:0010290 GO:0015399 GO:0015405 GO:0015431 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022804 GO:0022857 GO:0030054 GO:0031090 GO:0042623 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0048046 GO:0051179 GO:0051234 GO:0055044 GO:0055085 GO:0071997 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

ko01523 ko01524 ko02010 ko04071 ko04976 ko04977 ko05206 map01523 map01524 map02010 map04071 map04976 map04977 map05206

Pfam Domains

Protein Families

ABC_membrane ABC_tran

Protein Analysis

1456

Amino Acids

163.53

Weight (kDa)

8.86

Isoelectric Point (pI)

34.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ABC_membrane	PF00664	272 - 537	8.1e-24	ABC transporter transmembrane region
ABC_tran	PF00005	609 - 739	4.1e-14	ABC transporter
ABC_membrane	PF00664	902 - 1127	2.6e-28	ABC transporter transmembrane region
ABC_tran	PF00005	1217 - 1365	3.3e-29	ABC transporter

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0001408)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g24630
malus_domestica	MD13G1109600.v1.1 MD13G1110900.v1.1 MD14G1118700.v1.1 MD16G1109500.v1.1
prunus_persica	Prupe.1G235800_v2.0.a1 Prupe.1G236100_v2.0.a1
pyrus_communis	pycom11g17660 pycom13g09660
rosa_chinensis	RchiOBHm_Chr2g0131921 RchiOBHm_Chr4g0431141 RchiOBHm_Chr4g0431381 RchiOBHm_Chr4g0431551 RchiOBHm_Chr4g0431711 RchiOBHm_Chr5g0037421 RchiOBHm_Chr6g0306331
rosa_laevigata	RLG00000006895 RLG00000010802
rosa_multiflora	Rmu_co8343513.1_g000001 Rmu_sc0002271.1_g000023 Rmu_sc0002521.1_g000013 Rmu_sc0003473.1_g000005 Rmu_sc0005185.1_g000002 Rmu_sc0005258.1_g000003 Rmu_sc0013042.1_g000001
rosa_roxburghii	Rroxscaffold_3G00259720 Rroxscaffold_5G00372860 Rroxscaffold_5G00372880 Rroxscaffold_5G00372890 Rroxscaffold_5G00372970 Rroxscaffold_5G00373130 Rroxscaffold_7G00161860 Rroxscaffold_7G00161870
rosa_rugosa	Rorug04G0248700 Rorug04G0250100.1 Rorug04G0251800 Rorug04G0251800 Rorug04G0251900 Rorug04G0252000 Rorug04G0252100
rosa_samantha	Rh4AG308400 Rh4BG312300 Rh4BG312400 Rh4BG313800 Rh4BG316200 Rh4CG328600 Rh4DG308800 Rh4DG311000
rosa_wichuraiana	Rw4G026510 Rw4G026750 Rw4G026820 Rw5G016080 Rw6G040190

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2078
Acc36I	ACCTGC	1 cut(s)	1629
AccB1I	GGYRCC	1 cut(s)	3541
AccB7I	CCANNNNNTGG	2 cut(s)	664, 1088
AccBSI	CCGCTC	2 cut(s)	119, 3508
AccI	GTMKAC	4 cut(s)	1900, 2967, 3348, 3565
AccII	CGCG	1 cut(s)	3279
AcoI	YGGCCR	3 cut(s)	270, 1281, 3588
AcuI	CTGAAG	8 cut(s)	1160, 1379, 1403, 1469, 2865, 3434, 3727, 4014
AdeI	CACNNNGTG	1 cut(s)	1973
AfaI	GTAC	5 cut(s)	1017, 1148, 1567, 2180, 4244
AhdI	GACNNNNNGTC	1 cut(s)	3820
AjnI	CCWGG	8 cut(s)	1930, 2073, 2685, 2741, 3053, 3140, 3673, 4125
AjuI	GAANNNNNNNTTGG	2 cut(s)	1495, 1527
Alw21I	GWGCWC	6 cut(s)	310, 1003, 1632, 2650, 2896, 4053
Alw26I	GTCTC	7 cut(s)	13, 1128, 1748, 2267, 2864, 3048, 4124
AlwNI	CAGNNNCTG	1 cut(s)	348
Ama87I	CYCGRG	2 cut(s)	3760, 4044
AoxI	GGCC	9 cut(s)	270, 329, 1188, 1281, 1928, 3051, 3232, 3581, 3588
ArsI	GACNNNNNNTTYG	2 cut(s)	250, 282
AseI	ATTAAT	1 cut(s)	1101
Asp700I	GAANNNNTTC	1 cut(s)	3612
AspA2I	CCTAGG	1 cut(s)	3383
AspLEI	GCGC	2 cut(s)	3574, 4358
AspS9I	GGNCC	7 cut(s)	337, 857, 1079, 1928, 2071, 3876, 3964
AsuC2I	CCSGG	1 cut(s)	2657
AsuHPI	GGTGA	4 cut(s)	145, 1491, 1919, 3385
AsuII	TTCGAA	2 cut(s)	23, 3157
AvaI	CYCGRG	2 cut(s)	3760, 4044
AvaII	GGWCC	6 cut(s)	337, 857, 1079, 2071, 3876, 3964
AvrII	CCTAGG	1 cut(s)	3383
BaeI	ACNNNNGTAYC	2 cut(s)	1641, 1674
BalI	TGGCCA	2 cut(s)	272, 1283
BamHI	GGATCC	2 cut(s)	2398, 3886
BanI	GGYRCC	1 cut(s)	3541
BanII	GRGCYC	2 cut(s)	310, 1632
BauI	CACGAG	1 cut(s)	2169
BbsI	GAAGAC	6 cut(s)	129, 765, 2291, 2937, 3055, 4002
Bbv12I	GWGCWC	6 cut(s)	310, 1003, 1632, 2650, 2896, 4053
BceAI	ACGGC	2 cut(s)	855, 2566
BciT130I	CCWGG	8 cut(s)	1932, 2075, 2687, 2743, 3055, 3142, 3675, 4127
BciVI	GTATCC	2 cut(s)	1682, 1835
BclI	TGATCA	3 cut(s)	1107, 3363, 3907
BcnI	CCSGG	1 cut(s)	2657
BcoDI	GTCTC	7 cut(s)	13, 1128, 1748, 2267, 2864, 3048, 4124
BfaI	CTAG	8 cut(s)	447, 1508, 2925, 3129, 3137, 3384, 3402, 4214
BfmI	CTRYAG	3 cut(s)	3809, 4089, 4336
BfoI	RGCGCY	1 cut(s)	3575
BfuAI	ACCTGC	1 cut(s)	1629
BfuI	GTATCC	2 cut(s)	1682, 1835
BglI	GCCNNNNNGGC	1 cut(s)	3580
BglII	AGATCT	1 cut(s)	4316
BlnI	CCTAGG	1 cut(s)	3383
Bme1390I	CCNGG	9 cut(s)	1932, 2075, 2657, 2687, 2743, 3055, 3142, 3675, 4127
Bme18I	GGWCC	6 cut(s)	337, 857, 1079, 2071, 3876, 3964
BmeRI	GACNNNNNGTC	1 cut(s)	3820
BmeT110I	CYCGRG	2 cut(s)	3760, 4044
BmgT120I	GGNCC	7 cut(s)	337, 857, 1079, 1928, 2071, 3876, 3964
BmiI	GGNNCC	6 cut(s)	338, 1080, 2400, 3543, 3868, 3888
BmrFI	CCNGG	9 cut(s)	1932, 2075, 2657, 2687, 2743, 3055, 3142, 3675, 4127
BmrI	ACTGGG	1 cut(s)	3717
BmsI	GCATC	6 cut(s)	854, 1167, 2218, 3036, 3970, 4145
BmuI	ACTGGG	1 cut(s)	3717
BoxI	GACNNNNGTC	1 cut(s)	2425
BpiI	GAAGAC	6 cut(s)	129, 765, 2291, 2937, 3055, 4002
BplI	GAGNNNNNCTC	2 cut(s)	1017, 1049
BpmI	CTGGAG	3 cut(s)	35, 4030, 4236
Bpu10I	CCTNAGC	1 cut(s)	1424
Bpu14I	TTCGAA	2 cut(s)	23, 3157
BpuEI	CTTGAG	6 cut(s)	163, 566, 3030, 3635, 3838, 4286
BpuMI	CCSGG	1 cut(s)	2657
Bsa29I	ATCGAT	2 cut(s)	1661, 3191
BsaBI	GATNNNNATC	1 cut(s)	1748
BsaI	GGTCTC	1 cut(s)	4124
BsaWI	WCCGGW	1 cut(s)	2237
BsaXI	ACNNNNNCTCC	2 cut(s)	1926, 1956
Bse1I	ACTGG	6 cut(s)	18, 1993, 2596, 2717, 3712, 4013
Bse3DI	GCAATG	3 cut(s)	399, 1093, 3484
Bse8I	GATNNNNATC	1 cut(s)	1748
BseBI	CCWGG	8 cut(s)	1932, 2075, 2687, 2743, 3055, 3142, 3675, 4127
BseCI	ATCGAT	2 cut(s)	1661, 3191
BseJI	GATNNNNATC	1 cut(s)	1748
BseMI	GCAATG	3 cut(s)	399, 1093, 3484
BseMII	CTCAG	8 cut(s)	264, 355, 711, 731, 1438, 1878, 2381, 2867
BseNI	ACTGG	6 cut(s)	18, 1993, 2596, 2717, 3712, 4013
BseRI	GAGGAG	1 cut(s)	2157
BsgI	GTGCAG	2 cut(s)	1239, 2415
Bsh1236I	CGCG	1 cut(s)	3279
BshFI	GGCC	9 cut(s)	272, 331, 1190, 1283, 1930, 3053, 3234, 3583, 3590
BshNI	GGYRCC	1 cut(s)	3541
BshVI	ATCGAT	2 cut(s)	1661, 3191
BsiHKAI	GWGCWC	6 cut(s)	310, 1003, 1632, 2650, 2896, 4053
BsiHKCI	CYCGRG	2 cut(s)	3760, 4044
BsiSI	CCGG	5 cut(s)	828, 2238, 2657, 3096, 3591
BslFI	GGGAC	3 cut(s)	2263, 2639, 2703
BsmAI	GTCTC	7 cut(s)	13, 1128, 1748, 2267, 2864, 3048, 4124
BsmBI	CGTCTC	1 cut(s)	2864
BsmFI	GGGAC	3 cut(s)	2263, 2639, 2703
BsmI	GAATGC	3 cut(s)	1384, 3901, 4283
BsnI	GGCC	9 cut(s)	272, 331, 1190, 1283, 1930, 3053, 3234, 3583, 3590
Bso31I	GGTCTC	1 cut(s)	4124
BsoBI	CYCGRG	2 cut(s)	3760, 4044
Bsp119I	TTCGAA	2 cut(s)	23, 3157
Bsp1286I	GDGCHC	6 cut(s)	310, 1003, 1632, 2650, 2896, 4053
Bsp1407I	TGTACA	1 cut(s)	2178
Bsp19I	CCATGG	1 cut(s)	922
BspANI	GGCC	9 cut(s)	272, 331, 1190, 1283, 1930, 3053, 3234, 3583, 3590
BspCNI	CTCAG	8 cut(s)	263, 354, 710, 732, 1437, 1877, 2380, 2866
BspDI	ATCGAT	2 cut(s)	1661, 3191
BspFNI	CGCG	1 cut(s)	3279
BspHI	TCATGA	2 cut(s)	1740, 2572
BspLI	GGNNCC	6 cut(s)	338, 1080, 2400, 3543, 3868, 3888
BspMAI	CTGCAG	1 cut(s)	3813
BspMI	ACCTGC	1 cut(s)	1629
BspQI	GCTCTTC	2 cut(s)	1245, 1355
BspT104I	TTCGAA	2 cut(s)	23, 3157
BspT107I	GGYRCC	1 cut(s)	3541
BspTNI	GGTCTC	1 cut(s)	4124
BsrBI	CCGCTC	2 cut(s)	119, 3508
BsrDI	GCAATG	3 cut(s)	399, 1093, 3484
BsrGI	TGTACA	1 cut(s)	2178
BsrI	ACTGG	6 cut(s)	18, 1993, 2596, 2717, 3712, 4013
BssNAI	GTATAC	1 cut(s)	1901
BssSI	CACGAG	1 cut(s)	2169
BssT1I	CCWWGG	6 cut(s)	190, 332, 922, 1191, 2866, 3383
Bst1107I	GTATAC	1 cut(s)	1901
Bst2BI	CACGAG	1 cut(s)	2169
Bst2UI	CCWGG	8 cut(s)	1932, 2075, 2687, 2743, 3055, 3142, 3675, 4127
Bst6I	CTCTTC	9 cut(s)	769, 1245, 1355, 1445, 1493, 2571, 2687, 2867, 3746
BstAPI	GCANNNNNTGC	2 cut(s)	2837, 3989
BstAUI	TGTACA	1 cut(s)	2178
BstBI	TTCGAA	2 cut(s)	23, 3157
BstC8I	GCNNGC	3 cut(s)	789, 2555, 3380
BstDSI	CCRYGG	2 cut(s)	922, 4161
BstEII	GGTNACC	1 cut(s)	151
BstENI	CCTNNNNNAGG	1 cut(s)	2684
BstFNI	CGCG	1 cut(s)	3279
BstH2I	RGCGCY	1 cut(s)	3575
BstHHI	GCGC	2 cut(s)	3574, 4358
BstMAI	GTCTC	7 cut(s)	13, 1128, 1748, 2267, 2864, 3048, 4124
BstNI	CCWGG	8 cut(s)	1932, 2075, 2687, 2743, 3055, 3142, 3675, 4127
BstNSI	RCATGY	1 cut(s)	1470
BstPAI	GACNNNNGTC	1 cut(s)	2425
BstPI	GGTNACC	1 cut(s)	151
BstSCI	CCNGG	9 cut(s)	1930, 2073, 2655, 2685, 2741, 3053, 3140, 3673, 4125
BstSFI	CTRYAG	3 cut(s)	3809, 4089, 4336
BstUI	CGCG	1 cut(s)	3279
BstV2I	GAAGAC	6 cut(s)	129, 765, 2291, 2937, 3055, 4002
BstX2I	RGATCY	5 cut(s)	354, 2398, 3886, 4316, 4342
BstXI	CCANNNNNNTGG	5 cut(s)	327, 787, 1255, 1315, 4297
BstYI	RGATCY	5 cut(s)	354, 2398, 3886, 4316, 4342
BstZ17I	GTATAC	1 cut(s)	1901
Bsu15I	ATCGAT	2 cut(s)	1661, 3191
BsuI	GTATCC	2 cut(s)	1682, 1835
BsuRI	GGCC	9 cut(s)	272, 331, 1190, 1283, 1930, 3053, 3234, 3583, 3590
BsuTUI	ATCGAT	2 cut(s)	1661, 3191
BtgI	CCRYGG	2 cut(s)	922, 4161
BtsIMutI	CAGTG	9 cut(s)	11, 319, 737, 992, 1035, 1248, 2224, 2413, 4167
BveI	ACCTGC	1 cut(s)	1629
Cac8I	GCNNGC	3 cut(s)	789, 2555, 3380
CaiI	CAGNNNCTG	1 cut(s)	348
CciI	TCATGA	2 cut(s)	1740, 2572
CfoI	GCGC	2 cut(s)	3574, 4358
Cfr13I	GGNCC	7 cut(s)	337, 857, 1079, 1928, 2071, 3876, 3964
ClaI	ATCGAT	2 cut(s)	1661, 3191
CseI	GACGC	1 cut(s)	2846
Csp6I	GTAC	5 cut(s)	1016, 1147, 1566, 2179, 4243
CspCI	CAANNNNNGTGG	2 cut(s)	968, 1003
CviQI	GTAC	5 cut(s)	1016, 1147, 1566, 2179, 4243
DraI	TTTAAA	1 cut(s)	1377
DraIII	CACNNNGTG	1 cut(s)	1973
DrdI	GACNNNNNNGTC	1 cut(s)	2078
DriI	GACNNNNNGTC	1 cut(s)	3820
DseDI	GACNNNNNNGTC	1 cut(s)	2078
EaeI	YGGCCR	3 cut(s)	270, 1281, 3588
Eam1104I	CTCTTC	9 cut(s)	769, 1245, 1355, 1445, 1493, 2571, 2687, 2867, 3746
Eam1105I	GACNNNNNGTC	1 cut(s)	3820
EarI	CTCTTC	9 cut(s)	769, 1245, 1355, 1445, 1493, 2571, 2687, 2867, 3746
Ecl136II	GAGCTC	2 cut(s)	308, 1630
Eco130I	CCWWGG	6 cut(s)	190, 332, 922, 1191, 2866, 3383
Eco24I	GRGCYC	2 cut(s)	310, 1632
Eco31I	GGTCTC	1 cut(s)	4124
Eco32I	GATATC	1 cut(s)	3195
Eco47I	GGWCC	6 cut(s)	337, 857, 1079, 2071, 3876, 3964
Eco53kI	GAGCTC	2 cut(s)	308, 1630
Eco57I	CTGAAG	8 cut(s)	1160, 1379, 1403, 1469, 2865, 3434, 3727, 4014
Eco88I	CYCGRG	2 cut(s)	3760, 4044
Eco91I	GGTNACC	1 cut(s)	151
EcoICRI	GAGCTC	2 cut(s)	308, 1630
EcoNI	CCTNNNNNAGG	1 cut(s)	2684
EcoO109I	RGGNCCY	2 cut(s)	337, 857
EcoO65I	GGTNACC	1 cut(s)	151
EcoRI	GAATTC	5 cut(s)	1679, 2157, 3153, 3771, 4304
EcoRII	CCWGG	8 cut(s)	1930, 2073, 2685, 2741, 3053, 3140, 3673, 4125
EcoRV	GATATC	1 cut(s)	3195
EcoT14I	CCWWGG	6 cut(s)	190, 332, 922, 1191, 2866, 3383
EcoT38I	GRGCYC	2 cut(s)	310, 1632
ErhI	CCWWGG	6 cut(s)	190, 332, 922, 1191, 2866, 3383
Esp3I	CGTCTC	1 cut(s)	2864
FalI	AAGNNNNNCTT	2 cut(s)	316, 348
FaqI	GGGAC	3 cut(s)	2263, 2639, 2703
FauI	CCCGC	3 cut(s)	642, 1179, 3403
FbaI	TGATCA	3 cut(s)	1107, 3363, 3907
FblI	GTMKAC	4 cut(s)	1900, 2967, 3348, 3565
FriOI	GRGCYC	2 cut(s)	310, 1632
FspBI	CTAG	8 cut(s)	447, 1508, 2925, 3129, 3137, 3384, 3402, 4214
GlaI	GCGC	2 cut(s)	3573, 4357
GsuI	CTGGAG	3 cut(s)	35, 4030, 4236
HaeII	RGCGCY	1 cut(s)	3575
HaeIII	GGCC	9 cut(s)	272, 331, 1190, 1283, 1930, 3053, 3234, 3583, 3590
HapII	CCGG	5 cut(s)	828, 2238, 2657, 3096, 3591
HgaI	GACGC	1 cut(s)	2846
HhaI	GCGC	2 cut(s)	3574, 4358
Hin6I	GCGC	2 cut(s)	3572, 4356
HinP1I	GCGC	2 cut(s)	3572, 4356
HincII	GTYRAC	1 cut(s)	2458
HindII	GTYRAC	1 cut(s)	2458
HindIII	AAGCTT	4 cut(s)	181, 1956, 2555, 3108
HpaI	GTTAAC	1 cut(s)	2458
HpaII	CCGG	5 cut(s)	828, 2238, 2657, 3096, 3591
HphI	GGTGA	4 cut(s)	145, 1491, 1919, 3385
Hpy99I	CGWCG	1 cut(s)	734
HpyCH4IV	ACGT	3 cut(s)	53, 510, 1149
HpySE526I	ACGT	3 cut(s)	53, 510, 1149
HspAI	GCGC	2 cut(s)	3572, 4356
Ksp22I	TGATCA	3 cut(s)	1107, 3363, 3907
KspAI	GTTAAC	1 cut(s)	2458
LguI	GCTCTTC	2 cut(s)	1245, 1355
LmnI	GCTCC	3 cut(s)	1173, 1934, 3169
LweI	GCATC	6 cut(s)	854, 1167, 2218, 3036, 3970, 4145
MaeI	CTAG	8 cut(s)	447, 1508, 2925, 3129, 3137, 3384, 3402, 4214
MaeII	ACGT	3 cut(s)	53, 510, 1149
MbiI	CCGCTC	2 cut(s)	119, 3508
MflI	RGATCY	5 cut(s)	354, 2398, 3886, 4316, 4342
MhlI	GDGCHC	6 cut(s)	310, 1003, 1632, 2650, 2896, 4053
MlsI	TGGCCA	2 cut(s)	272, 1283
MluNI	TGGCCA	2 cut(s)	272, 1283
MlyI	GAGTC	5 cut(s)	755, 840, 985, 1403, 2906
MmeI	TCCRAC	3 cut(s)	3130, 3997, 4059
Mox20I	TGGCCA	2 cut(s)	272, 1283
MroXI	GAANNNNTTC	1 cut(s)	3612
MscI	TGGCCA	2 cut(s)	272, 1283
MslI	CAYNNNNRTG	5 cut(s)	622, 1637, 3038, 3294, 3371
Msp20I	TGGCCA	2 cut(s)	272, 1283
MspA1I	CMGCKG	3 cut(s)	2729, 2758, 4332
MspI	CCGG	5 cut(s)	828, 2238, 2657, 3096, 3591
MspR9I	CCNGG	9 cut(s)	1932, 2075, 2657, 2687, 2743, 3055, 3142, 3675, 4127
Mva1269I	GAATGC	3 cut(s)	1384, 3901, 4283
MvaI	CCWGG	8 cut(s)	1932, 2075, 2687, 2743, 3055, 3142, 3675, 4127
MvnI	CGCG	1 cut(s)	3279
NciI	CCSGG	1 cut(s)	2657
NcoI	CCATGG	1 cut(s)	922
NlaIV	GGNNCC	6 cut(s)	338, 1080, 2400, 3543, 3868, 3888
NmeAIII	GCCGAG	1 cut(s)	1451
NmuCI	GTSAC	4 cut(s)	151, 578, 2626, 4165
NspI	RCATGY	1 cut(s)	1470
NspV	TTCGAA	2 cut(s)	23, 3157
PaeR7I	CTCGAG	1 cut(s)	4044
PagI	TCATGA	2 cut(s)	1740, 2572
PciSI	GCTCTTC	2 cut(s)	1245, 1355
PctI	GAATGC	3 cut(s)	1384, 3901, 4283
PdmI	GAANNNNTTC	1 cut(s)	3612
PflFI	GACNNNGTC	1 cut(s)	2336
PflMI	CCANNNNNTGG	2 cut(s)	664, 1088
PfoI	TCCNGGA	1 cut(s)	4125
PleI	GAGTC	5 cut(s)	755, 839, 984, 1402, 2906
PpsI	GAGTC	5 cut(s)	755, 839, 984, 1402, 2906
PpuMI	RGGWCCY	2 cut(s)	337, 857
PshAI	GACNNNNGTC	1 cut(s)	2425
PshBI	ATTAAT	1 cut(s)	1101
Psp124BI	GAGCTC	2 cut(s)	310, 1632
Psp5II	RGGWCCY	2 cut(s)	337, 857
Psp6I	CCWGG	8 cut(s)	1930, 2073, 2685, 2741, 3053, 3140, 3673, 4125
PspEI	GGTNACC	1 cut(s)	151
PspGI	CCWGG	8 cut(s)	1930, 2073, 2685, 2741, 3053, 3140, 3673, 4125
PspN4I	GGNNCC	6 cut(s)	338, 1080, 2400, 3543, 3868, 3888
PspPI	GGNCC	7 cut(s)	337, 857, 1079, 1928, 2071, 3876, 3964
PspPPI	RGGWCCY	2 cut(s)	337, 857
PspXI	VCTCGAGB	1 cut(s)	4044
PstI	CTGCAG	1 cut(s)	3813
PstNI	CAGNNNCTG	1 cut(s)	348
PsuI	RGATCY	5 cut(s)	354, 2398, 3886, 4316, 4342
PsyI	GACNNNGTC	1 cut(s)	2336
PvuII	CAGCTG	1 cut(s)	4332
RsaI	GTAC	5 cut(s)	1017, 1148, 1567, 2180, 4244
RsaNI	GTAC	5 cut(s)	1016, 1147, 1566, 2179, 4243
RseI	CAYNNNNRTG	5 cut(s)	622, 1637, 3038, 3294, 3371
SacI	GAGCTC	2 cut(s)	310, 1632
SapI	GCTCTTC	2 cut(s)	1245, 1355
Sau96I	GGNCC	7 cut(s)	337, 857, 1079, 1928, 2071, 3876, 3964
SchI	GAGTC	5 cut(s)	755, 840, 985, 1403, 2906
ScrFI	CCNGG	9 cut(s)	1932, 2075, 2657, 2687, 2743, 3055, 3142, 3675, 4127
SduI	GDGCHC	6 cut(s)	310, 1003, 1632, 2650, 2896, 4053
SfaNI	GCATC	6 cut(s)	854, 1167, 2218, 3036, 3970, 4145
SfcI	CTRYAG	3 cut(s)	3809, 4089, 4336
Sfr274I	CTCGAG	1 cut(s)	4044
SfuI	TTCGAA	2 cut(s)	23, 3157
SinI	GGWCC	6 cut(s)	337, 857, 1079, 2071, 3876, 3964
SlaI	CTCGAG	1 cut(s)	4044
SmiMI	CAYNNNNRTG	5 cut(s)	622, 1637, 3038, 3294, 3371
SmlI	CTYRAG	7 cut(s)	178, 545, 3045, 3650, 3817, 4044, 4265
SmoI	CTYRAG	7 cut(s)	178, 545, 3045, 3650, 3817, 4044, 4265
SspMI	CTAG	8 cut(s)	447, 1508, 2925, 3129, 3137, 3384, 3402, 4214
SstI	GAGCTC	2 cut(s)	310, 1632
StyD4I	CCNGG	9 cut(s)	1930, 2073, 2655, 2685, 2741, 3053, 3140, 3673, 4125
StyI	CCWWGG	6 cut(s)	190, 332, 922, 1191, 2866, 3383
TaiI	ACGT	3 cut(s)	56, 513, 1152
TatI	WGTACW	1 cut(s)	2178
TauI	GCSGC	1 cut(s)	3237
TscAI	CASTG	9 cut(s)	18, 319, 744, 992, 1042, 1255, 2224, 2413, 4174
TseFI	GTSAC	4 cut(s)	151, 578, 2626, 4165
Tsp45I	GTSAC	4 cut(s)	151, 578, 2626, 4165
TspGWI	ACGGA	2 cut(s)	3962, 4014
TspRI	CASTG	9 cut(s)	18, 319, 744, 992, 1042, 1255, 2224, 2413, 4174
Tth111I	GACNNNGTC	1 cut(s)	2336
Van91I	CCANNNNNTGG	2 cut(s)	664, 1088
VpaK11BI	GGWCC	6 cut(s)	337, 857, 1079, 2071, 3876, 3964
VspI	ATTAAT	1 cut(s)	1101
XagI	CCTNNNNNAGG	1 cut(s)	2684
XceI	RCATGY	1 cut(s)	1470
XcmI	CCANNNNNNNNNTGG	3 cut(s)	1974, 2806, 3576
XhoI	CTCGAG	1 cut(s)	4044
XmaJI	CCTAGG	1 cut(s)	3383
XmiI	GTMKAC	4 cut(s)	1900, 2967, 3348, 3565
XmnI	GAANNNNTTC	1 cut(s)	3612
XspI	CTAG	8 cut(s)	447, 1508, 2925, 3129, 3137, 3384, 3402, 4214

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.