Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0001409

Physical Location & Seq

Genomic Coordinates Reverse (-)

15422816 .. 15429100

Gene Structure 6285 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1158900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1671 bp

ATGAGAATTCAGTTTGCAAAGGAGAATCCATGTGCCACTAATCTTCCACAACTCAAAAATGAAGATGGGGGATGGGGTCTGCATATTGAGGGGCCAAGTACCATGTTTGGCACTGCACTTAATTATGTCACTTTGAGGTTGCTTGGTGAAGGTGCTGATGATGGACAAGGGGCAATCGAACTAGCACGCAAGTGGATATTAGACCATGGTGGCGTACTTGGGGCATATGAATGGTCTGGGAATAATCCACTTCCGCCTGAAGTGTGGCTATGTCCTTACTCTCTTCCATTTCATCCAGGAAGAATGTGGTGTCATTGCCGAATGGTGTATTTGCCCATTTCATATTTGTATGGGAAGAGGTTTGTTGGACCTATCACACCCACAATCCGGTCATTGGGGAAGGAGCTTTATACAGTCCCCTATCATGAAGTTGATTGGCATAATGCTCGCAATCTATGTGCAAGGGTTGATCTATACTATCTGCACCCAATGGTACAAGATATCTTGTGGGGGTCTCTTCACTATGTTTATGAACCTATTTTCACACGTTGGCCAGCAAAAGAATTGAGGAAGAGTTCTTTGAAGACTGTAATGCAGCATATACATTATGAAGATGAAAATACTCGGTACTTATGCATAGGGCCTGTTAACAAGGTGTTAAATATGCTTTGTTGTTGGGCGGAGGATCCAAACTCAGAGGCATCCAAGTTGCATCTCCCGAGAATCCCTGATTATTTATGGATCGCTGAAGATGGCATGAAGATGCAGGGCTACAATGGAAGTCAAGCATGGGATACTTCTTTTGCCATTCAAGCGATCATATCAACTAACCTTGTTTTAGAAGATTGTCCTGGAGATCGGAACTTTTGGTATCGTCACATTTCAAAAGGTGCTTGGCCATTCTCAATAGCCGATCATGGATGGCCTATCTCTGACTGCACAGCAGAAGGGCTATATGTTGAATGCACCTCAGCGGCAATTCAAGCACTTGCATTGTTCAAAAAATTATGTCCTGGGCATAGGAGTGAAGAAATAGAAAACTGTATTGCTCGTGCTGCAAAGTTCATTGAAACGATACAAGCAACGGATGGATCTTGGTATGGTTCCTGGGGAGCATGCTTCACCTATGCTGGATGGTTTGGAATAAAGGGGTTGGTTGCTGCGGGAAGGACATACCGCTCTAGCATTTGTAAAGCATGTGACTTTTTGTTATCCAAAGAGCTTCCTTCAGGTGGATGGGGAGAAAGCTATCTATCATGTCAAAACAAGGTGTACACAAATCTCAAGGACAATAGGCCACATATAGTTCATACTGAATGGGCTATGTTGGCCCTCATTGGTGCCGGGCAGGTACATTTGGACATCAAATTCATCTCTCCTCCTTCCCATGCGATTTATCAAATTTATCCTTCAAGTTCTTCATCCACTTATCGAACTGGCTGCTGGCTGCTGCATAATGAATCAATGCTTCTGGCTGCTGCAGACCCAACTCCGTTGCATCGGGCATTTAGGGTACCAATCAATTCTCAAATGGAAAATGGAGATTTCCCTCAAAAGGAGATCACTGGTGTCTTCAACAAGAACTGTATGATCAGCTATTCAGCCTACCGAAACATTTTTCCCGTTTGGGCCCTCGGAGAATATCGTTGCCAGGTGTTGGACGCCCTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00100 ko01100 ko01110 map00100 map01100 map01110

Pfam Domains

Protein Families

SQHop_cyclase_C SQHop_cyclase_N

Protein Analysis

557

Amino Acids

62.91

Weight (kDa)

6.47

Isoelectric Point (pI)

41.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SQHop_cyclase_N	PF13249	7 - 236	4.7e-20	Squalene-hopene cyclase N-terminal domain
SQHop_cyclase_C	PF13243	342 - 452	2.1e-18	Squalene-hopene cyclase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001409)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g10860 FvH4_7g10860 FvH4_7g10860
malus_domestica	MD17G1158900.v1.1 MD17G1160000.v1.1 MD17G1246000.v1.1 MD17G1246100.v1.1 MD17G1246500.v1.1 MD17G1246700.v1.1
prunus_persica	Prupe.1G002600_v2.0.a1 Prupe.1G002600_v2.0.a1 Prupe.7G043400_v2.0.a1
pyrus_communis	pycom16g19870 pycom16g24410
rosa_chinensis	RchiOBHm_Chr1g0332711 RchiOBHm_Chr2g0148781 RchiOBHm_Chr4g0385071 RchiOBHm_Chr4g0385101
rosa_laevigata	RLG00000010257
rosa_multiflora	Rmu_co8015556.1_g000001 Rmu_co8308753.1_g000001 Rmu_co8434809.1_g000001 Rmu_sc0000824.1_g000004 Rmu_sc0001548.1_g000031 Rmu_sc0001548.1_g000053 Rmu_sc0002070.1_g000040 Rmu_sc0002116.1_g000014 Rmu_sc0003929.1_g000004 Rmu_sc0008546.1_g000012 Rmu_sc0008678.1_g000005 Rmu_ssc0000158.1_g000036
rosa_roxburghii	Rroxscaffold_5G00333010 Rroxscaffold_5G00342310
rosa_rugosa	Rorug03G0113700 Rorug03G0225800 Rorug03G0298900
rosa_samantha	Rh1CG121300 Rh1DG132500 Rh2AG466900 Rh2CG453300 Rh2CG453700 Rh4AG002600 Rh4AG078200 Rh4AG078500 Rh4BG002100 Rh4BG073600 Rh4BG073800 Rh4BG075600 Rh4BG076300 Rh4DG002100 Rh4DG071700 Rh7BG219000
rosa_wichuraiana	Rw4G000670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1339
Acc65I	GGTACC	1 cut(s)	1513
AccB1I	GGYRCC	2 cut(s)	1340, 1513
AccB7I	CCANNNNNTGG	1 cut(s)	1657
AccBSI	CCGCTC	1 cut(s)	1179
AciI	CCGC	5 cut(s)	254, 680, 974, 1163, 1177
AclWI	GGATC	4 cut(s)	680, 693, 749, 1099
AcoI	YGGCCR	2 cut(s)	551, 896
AcsI	RAATTY	3 cut(s)	6, 1367, 1401
AcuI	CTGAAG	3 cut(s)	279, 768, 1212
AcyI	GRCGYC	1 cut(s)	1662
AfaI	GTAC	7 cut(s)	100, 216, 495, 629, 1274, 1353, 1515
AfiI	CCNNNNNNNGG	5 cut(s)	387, 394, 1232, 1555, 1657
AflIII	ACRYGT	1 cut(s)	545
AgsI	TTSAA	9 cut(s)	583, 812, 885, 962, 983, 1000, 1070, 1413, 1576
AjnI	CCWGG	5 cut(s)	295, 850, 1012, 1106, 1650
AleI	CACNNNNGTG	1 cut(s)	190
AluBI	AGCT	4 cut(s)	406, 1222, 1248, 1596
AluI	AGCT	4 cut(s)	406, 1222, 1248, 1596
Alw26I	GTCTC	1 cut(s)	519
AlwI	GGATC	4 cut(s)	680, 693, 749, 1099
Ama87I	CYCGRG	1 cut(s)	718
AoxI	GGCC	8 cut(s)	92, 551, 641, 896, 923, 1295, 1329, 1629
ApaI	GGGCCC	1 cut(s)	1633
ApeKI	GCWGC	8 cut(s)	595, 1055, 1160, 1440, 1447, 1450, 1475, 1478
ApoI	RAATTY	3 cut(s)	6, 1367, 1401
Asp718I	GGTACC	1 cut(s)	1513
AspS9I	GGNCC	6 cut(s)	92, 368, 641, 1330, 1629, 1630
AsuC2I	CCSGG	1 cut(s)	1345
AsuHPI	GGTGA	2 cut(s)	158, 1114
AvaI	CYCGRG	1 cut(s)	718
AvaII	GGWCC	1 cut(s)	368
BaeGI	GKGCMC	1 cut(s)	1633
BalI	TGGCCA	2 cut(s)	553, 898
BamHI	GGATCC	1 cut(s)	685
BanI	GGYRCC	2 cut(s)	1340, 1513
BanII	GRGCYC	1 cut(s)	1633
BauI	CACGAG	1 cut(s)	1050
BbsI	GAAGAC	2 cut(s)	590, 1564
BbvCI	CCTCAGC	1 cut(s)	970
BbvI	GCAGC	8 cut(s)	607, 1042, 1147, 1427, 1434, 1437, 1462, 1465
BccI	CCATC	8 cut(s)	59, 66, 155, 746, 915, 1082, 1128, 1230
BcgI	CGANNNNNNTGC	4 cut(s)	428, 462, 1422, 1456
BciT130I	CCWGG	5 cut(s)	297, 852, 1014, 1108, 1652
BciVI	GTATCC	1 cut(s)	787
BclI	TGATCA	1 cut(s)	1590
BcnI	CCSGG	1 cut(s)	1345
BcoDI	GTCTC	1 cut(s)	519
BfaI	CTAG	2 cut(s)	182, 1182
BfmI	CTRYAG	1 cut(s)	1479
BfuAI	ACCTGC	1 cut(s)	1339
BfuI	GTATCC	1 cut(s)	787
BisI	GCNGC	9 cut(s)	596, 975, 1056, 1161, 1441, 1448, 1451, 1476, 1479
BlsI	GCNGC	9 cut(s)	597, 976, 1057, 1162, 1442, 1449, 1452, 1477, 1480
Bme1390I	CCNGG	6 cut(s)	297, 852, 1014, 1108, 1345, 1652
Bme18I	GGWCC	1 cut(s)	368
BmeT110I	CYCGRG	1 cut(s)	718
BmgT120I	GGNCC	6 cut(s)	92, 368, 641, 1330, 1629, 1630
BmiI	GGNNCC	6 cut(s)	93, 687, 1105, 1342, 1515, 1631
BmrFI	CCNGG	6 cut(s)	297, 852, 1014, 1108, 1345, 1652
BmsI	GCATC	4 cut(s)	710, 721, 753, 1507
BpiI	GAAGAC	2 cut(s)	590, 1564
BpmI	CTGGAG	1 cut(s)	873
Bpu10I	CCTNAGC	1 cut(s)	970
BpuEI	CTTGAG	1 cut(s)	1268
BpuMI	CCSGG	1 cut(s)	1345
BsaHI	GRCGYC	1 cut(s)	1662
BsaI	GGTCTC	1 cut(s)	519
BsaJI	CCNNGG	4 cut(s)	205, 1013, 1107, 1633
BsaWI	WCCGGW	1 cut(s)	387
Bsc4I	CCNNNNNNNGG	5 cut(s)	387, 394, 1232, 1555, 1657
Bse1I	ACTGG	2 cut(s)	1441, 1570
Bse3DI	GCAATG	1 cut(s)	313
BseBI	CCWGG	5 cut(s)	297, 852, 1014, 1108, 1652
BseDI	CCNNGG	4 cut(s)	205, 1013, 1107, 1633
BseGI	GGATG	8 cut(s)	77, 292, 701, 926, 1093, 1139, 1241, 1421
BseLI	CCNNNNNNNGG	5 cut(s)	387, 394, 1232, 1555, 1657
BseMI	GCAATG	1 cut(s)	313
BseMII	CTCAG	2 cut(s)	708, 984
BseNI	ACTGG	2 cut(s)	1441, 1570
BseRI	GAGGAG	1 cut(s)	1368
BseSI	GKGCMC	1 cut(s)	1633
BseXI	GCAGC	8 cut(s)	607, 1042, 1147, 1427, 1434, 1437, 1462, 1465
BsgI	GTGCAG	3 cut(s)	99, 467, 922
BshFI	GGCC	8 cut(s)	94, 553, 643, 898, 925, 1297, 1331, 1631
BshNI	GGYRCC	2 cut(s)	1340, 1513
BsiHKCI	CYCGRG	1 cut(s)	718
BsiSI	CCGG	2 cut(s)	388, 1344
BslFI	GGGAC	1 cut(s)	401
BslI	CCNNNNNNNGG	5 cut(s)	387, 394, 1232, 1555, 1657
BsmAI	GTCTC	1 cut(s)	519
BsmFI	GGGAC	1 cut(s)	401
BsmI	GAATGC	1 cut(s)	968
BsnI	GGCC	8 cut(s)	94, 553, 643, 898, 925, 1297, 1331, 1631
Bso31I	GGTCTC	1 cut(s)	519
BsoBI	CYCGRG	1 cut(s)	718
Bsp120I	GGGCCC	1 cut(s)	1629
Bsp1286I	GDGCHC	1 cut(s)	1633
Bsp1407I	TGTACA	1 cut(s)	1272
Bsp143I	GATC	9 cut(s)	469, 685, 741, 816, 856, 913, 1091, 1560, 1590
Bsp19I	CCATGG	1 cut(s)	205
BspACI	CCGC	5 cut(s)	254, 680, 974, 1163, 1177
BspANI	GGCC	8 cut(s)	94, 553, 643, 898, 925, 1297, 1331, 1631
BspCNI	CTCAG	2 cut(s)	707, 983
BspHI	TCATGA	1 cut(s)	424
BspLI	GGNNCC	6 cut(s)	93, 687, 1105, 1342, 1515, 1631
BspMAI	CTGCAG	1 cut(s)	1483
BspMI	ACCTGC	1 cut(s)	1339
BspPI	GGATC	4 cut(s)	680, 693, 749, 1099
BspT107I	GGYRCC	2 cut(s)	1340, 1513
BspTNI	GGTCTC	1 cut(s)	519
BsrBI	CCGCTC	1 cut(s)	1179
BsrDI	GCAATG	1 cut(s)	313
BsrGI	TGTACA	1 cut(s)	1272
BsrI	ACTGG	2 cut(s)	1441, 1570
BssECI	CCNNGG	4 cut(s)	205, 1013, 1107, 1633
BssMI	GATC	9 cut(s)	469, 685, 741, 816, 856, 913, 1091, 1560, 1590
BssNI	GRCGYC	1 cut(s)	1662
BssSI	CACGAG	1 cut(s)	1050
BssT1I	CCWWGG	1 cut(s)	205
Bst2BI	CACGAG	1 cut(s)	1050
Bst2UI	CCWGG	5 cut(s)	297, 852, 1014, 1108, 1652
Bst4CI	ACNGT	4 cut(s)	415, 589, 1043, 1586
Bst6I	CTCTTC	4 cut(s)	288, 350, 522, 566
BstACI	GRCGYC	1 cut(s)	1662
BstAUI	TGTACA	1 cut(s)	1272
BstC8I	GCNNGC	5 cut(s)	187, 448, 555, 1117, 1445
BstDEI	CTNAG	2 cut(s)	694, 970
BstDSI	CCRYGG	1 cut(s)	205
BstF5I	GGATG	8 cut(s)	77, 292, 701, 926, 1093, 1139, 1241, 1421
BstKTI	GATC	9 cut(s)	472, 688, 744, 819, 859, 916, 1094, 1563, 1593
BstMAI	GTCTC	1 cut(s)	519
BstMBI	GATC	9 cut(s)	469, 685, 741, 816, 856, 913, 1091, 1560, 1590
BstMWI	GCNNNNNNNGC	4 cut(s)	812, 983, 1055, 1328
BstNI	CCWGG	5 cut(s)	297, 852, 1014, 1108, 1652
BstNSI	RCATGY	2 cut(s)	1119, 1200
BstSCI	CCNGG	6 cut(s)	295, 850, 1012, 1106, 1343, 1650
BstSFI	CTRYAG	1 cut(s)	1479
BstSLI	GKGCMC	1 cut(s)	1633
BstV1I	GCAGC	8 cut(s)	607, 1042, 1147, 1427, 1434, 1437, 1462, 1465
BstV2I	GAAGAC	2 cut(s)	590, 1564
BstX2I	RGATCY	2 cut(s)	685, 1091
BstYI	RGATCY	2 cut(s)	685, 1091
BsuI	GTATCC	1 cut(s)	787
BsuRI	GGCC	8 cut(s)	94, 553, 643, 898, 925, 1297, 1331, 1631
BtgI	CCRYGG	1 cut(s)	205
BtsCI	GGATG	8 cut(s)	77, 292, 701, 926, 1093, 1139, 1241, 1421
BtsI	GCAGTG	1 cut(s)	111
BtsIMutI	CAGTG	2 cut(s)	111, 1563
BveI	ACCTGC	1 cut(s)	1339
Cac8I	GCNNGC	5 cut(s)	187, 448, 555, 1117, 1445
CciI	TCATGA	1 cut(s)	424
Cfr13I	GGNCC	6 cut(s)	92, 368, 641, 1330, 1629, 1630
Csp6I	GTAC	7 cut(s)	99, 215, 494, 628, 1273, 1352, 1514
CviQI	GTAC	7 cut(s)	99, 215, 494, 628, 1273, 1352, 1514
DdeI	CTNAG	2 cut(s)	694, 970
DpnI	GATC	9 cut(s)	471, 687, 743, 818, 858, 915, 1093, 1562, 1592
DpnII	GATC	9 cut(s)	469, 685, 741, 816, 856, 913, 1091, 1560, 1590
EaeI	YGGCCR	2 cut(s)	551, 896
Eam1104I	CTCTTC	4 cut(s)	288, 350, 522, 566
EarI	CTCTTC	4 cut(s)	288, 350, 522, 566
EciI	GGCGGA	2 cut(s)	243, 695
Eco130I	CCWWGG	1 cut(s)	205
Eco24I	GRGCYC	1 cut(s)	1633
Eco31I	GGTCTC	1 cut(s)	519
Eco32I	GATATC	1 cut(s)	502
Eco47I	GGWCC	1 cut(s)	368
Eco57I	CTGAAG	3 cut(s)	279, 768, 1212
Eco88I	CYCGRG	1 cut(s)	718
EcoO109I	RGGNCCY	2 cut(s)	641, 1630
EcoRI	GAATTC	1 cut(s)	6
EcoRII	CCWGG	5 cut(s)	295, 850, 1012, 1106, 1650
EcoRV	GATATC	1 cut(s)	502
EcoT14I	CCWWGG	1 cut(s)	205
EcoT22I	ATGCAT	1 cut(s)	638
EcoT38I	GRGCYC	1 cut(s)	1633
ErhI	CCWWGG	1 cut(s)	205
FaqI	GGGAC	1 cut(s)	401
FauI	CCCGC	1 cut(s)	1156
FauNDI	CATATG	1 cut(s)	226
FbaI	TGATCA	1 cut(s)	1590
Fnu4HI	GCNGC	9 cut(s)	596, 975, 1056, 1161, 1441, 1448, 1451, 1476, 1479
FokI	GGATG	8 cut(s)	84, 279, 688, 933, 1100, 1146, 1248, 1408
FriOI	GRGCYC	1 cut(s)	1633
Fsp4HI	GCNGC	9 cut(s)	596, 975, 1056, 1161, 1441, 1448, 1451, 1476, 1479
FspBI	CTAG	2 cut(s)	182, 1182
GluI	GCNGC	9 cut(s)	596, 975, 1056, 1161, 1441, 1448, 1451, 1476, 1479
GsuI	CTGGAG	1 cut(s)	873
HaeIII	GGCC	8 cut(s)	94, 553, 643, 898, 925, 1297, 1331, 1631
HapII	CCGG	2 cut(s)	388, 1344
Hin1I	GRCGYC	1 cut(s)	1662
HincII	GTYRAC	1 cut(s)	649
HindII	GTYRAC	1 cut(s)	649
HinfI	GANTC	3 cut(s)	25, 723, 1460
HpaI	GTTAAC	1 cut(s)	649
HpaII	CCGG	2 cut(s)	388, 1344
HphI	GGTGA	2 cut(s)	158, 1114
Hpy166II	GTNNAC	3 cut(s)	649, 1273, 1275
Hpy188I	TCNGA	5 cut(s)	697, 861, 934, 1637, 1670
Hpy188III	TCNNGA	2 cut(s)	425, 718
Hpy8I	GTNNAC	3 cut(s)	649, 1273, 1275
HpyAV	CCTTC	7 cut(s)	143, 394, 941, 1161, 1236, 1392, 1419
HpyCH4III	ACNGT	4 cut(s)	415, 589, 1043, 1586
HpyCH4IV	ACGT	1 cut(s)	547
HpyF10VI	GCNNNNNNNGC	4 cut(s)	812, 983, 1055, 1328
HpyF3I	CTNAG	2 cut(s)	694, 970
HpySE526I	ACGT	1 cut(s)	547
Hsp92I	GRCGYC	1 cut(s)	1662
KpnI	GGTACC	1 cut(s)	1517
Ksp22I	TGATCA	1 cut(s)	1590
KspAI	GTTAAC	1 cut(s)	649
Kzo9I	GATC	9 cut(s)	469, 685, 741, 816, 856, 913, 1091, 1560, 1590
LmnI	GCTCC	2 cut(s)	403, 1112
Lsp1109I	GCAGC	8 cut(s)	607, 1042, 1147, 1427, 1434, 1437, 1462, 1465
LweI	GCATC	4 cut(s)	710, 721, 753, 1507
MaeI	CTAG	2 cut(s)	182, 1182
MaeII	ACGT	1 cut(s)	547
MaeIII	GTNAC	3 cut(s)	127, 875, 1199
MalI	GATC	9 cut(s)	471, 687, 743, 818, 858, 915, 1093, 1562, 1592
MbiI	CCGCTC	1 cut(s)	1179
MboI	GATC	9 cut(s)	469, 685, 741, 816, 856, 913, 1091, 1560, 1590
MflI	RGATCY	2 cut(s)	685, 1091
MhlI	GDGCHC	1 cut(s)	1633
MlsI	TGGCCA	2 cut(s)	553, 898
MluCI	AATT	8 cut(s)	6, 121, 563, 978, 1004, 1367, 1401, 1522
MluNI	TGGCCA	2 cut(s)	553, 898
MmeI	TCCRAC	2 cut(s)	346, 1638
Mox20I	TGGCCA	2 cut(s)	553, 898
Mph1103I	ATGCAT	1 cut(s)	638
MscI	TGGCCA	2 cut(s)	553, 898
MseI	TTAA	3 cut(s)	120, 648, 659
MslI	CAYNNNNRTG	4 cut(s)	190, 229, 761, 1023
Msp20I	TGGCCA	2 cut(s)	553, 898
MspA1I	CMGCKG	1 cut(s)	974
MspI	CCGG	2 cut(s)	388, 1344
MspR9I	CCNGG	6 cut(s)	297, 852, 1014, 1108, 1345, 1652
Mva1269I	GAATGC	1 cut(s)	968
MvaI	CCWGG	5 cut(s)	297, 852, 1014, 1108, 1652
MwoI	GCNNNNNNNGC	4 cut(s)	812, 983, 1055, 1328
NciI	CCSGG	1 cut(s)	1345
NcoI	CCATGG	1 cut(s)	205
NdeI	CATATG	1 cut(s)	226
NdeII	GATC	9 cut(s)	469, 685, 741, 816, 856, 913, 1091, 1560, 1590
NlaIV	GGNNCC	6 cut(s)	93, 687, 1105, 1342, 1515, 1631
NmuCI	GTSAC	3 cut(s)	127, 875, 1199
NsiI	ATGCAT	1 cut(s)	638
NspI	RCATGY	2 cut(s)	1119, 1200
OliI	CACNNNNGTG	1 cut(s)	190
PaeI	GCATGC	1 cut(s)	1119
PagI	TCATGA	1 cut(s)	424
PctI	GAATGC	1 cut(s)	968
PfeI	GAWTC	3 cut(s)	25, 723, 1460
PflMI	CCANNNNNTGG	1 cut(s)	1657
PfoI	TCCNGGA	2 cut(s)	295, 850
PkrI	GCNGC	9 cut(s)	597, 976, 1057, 1162, 1442, 1449, 1452, 1477, 1480
Psp6I	CCWGG	5 cut(s)	295, 850, 1012, 1106, 1650
PspGI	CCWGG	5 cut(s)	295, 850, 1012, 1106, 1650
PspN4I	GGNNCC	6 cut(s)	93, 687, 1105, 1342, 1515, 1631
PspOMI	GGGCCC	1 cut(s)	1629
PspPI	GGNCC	6 cut(s)	92, 368, 641, 1330, 1629, 1630
PstI	CTGCAG	1 cut(s)	1483
PsuI	RGATCY	2 cut(s)	685, 1091
RsaI	GTAC	7 cut(s)	100, 216, 495, 629, 1274, 1353, 1515
RsaNI	GTAC	7 cut(s)	99, 215, 494, 628, 1273, 1352, 1514
RseI	CAYNNNNRTG	4 cut(s)	190, 229, 761, 1023
SaqAI	TTAA	3 cut(s)	120, 648, 659
SatI	GCNGC	9 cut(s)	596, 975, 1056, 1161, 1441, 1448, 1451, 1476, 1479
Sau3AI	GATC	9 cut(s)	469, 685, 741, 816, 856, 913, 1091, 1560, 1590
Sau96I	GGNCC	6 cut(s)	92, 368, 641, 1330, 1629, 1630
ScrFI	CCNGG	6 cut(s)	297, 852, 1014, 1108, 1345, 1652
SduI	GDGCHC	1 cut(s)	1633
SfaNI	GCATC	4 cut(s)	710, 721, 753, 1507
SfcI	CTRYAG	1 cut(s)	1479
SinI	GGWCC	1 cut(s)	368
SmiMI	CAYNNNNRTG	4 cut(s)	190, 229, 761, 1023
SmlI	CTYRAG	1 cut(s)	1283
SmoI	CTYRAG	1 cut(s)	1283
SphI	GCATGC	1 cut(s)	1119
Sse9I	AATT	8 cut(s)	6, 121, 563, 978, 1004, 1367, 1401, 1522
SsiI	CCGC	5 cut(s)	254, 680, 974, 1163, 1177
SspMI	CTAG	2 cut(s)	182, 1182
StyD4I	CCNGG	6 cut(s)	295, 850, 1012, 1106, 1343, 1650
StyI	CCWWGG	1 cut(s)	205
TaaI	ACNGT	4 cut(s)	415, 589, 1043, 1586
TaiI	ACGT	1 cut(s)	550
TaqI	TCGA	2 cut(s)	177, 1432
TasI	AATT	8 cut(s)	6, 121, 563, 978, 1004, 1367, 1401, 1522
TatI	WGTACW	1 cut(s)	1272
TauI	GCSGC	1 cut(s)	977
TfiI	GAWTC	3 cut(s)	25, 723, 1460
Tru1I	TTAA	3 cut(s)	120, 648, 659
Tru9I	TTAA	3 cut(s)	120, 648, 659
TscAI	CASTG	2 cut(s)	118, 1570
TseFI	GTSAC	3 cut(s)	127, 875, 1199
TseI	GCWGC	8 cut(s)	595, 1055, 1160, 1440, 1447, 1450, 1475, 1478
Tsp45I	GTSAC	3 cut(s)	127, 875, 1199
TspGWI	ACGGA	2 cut(s)	1100, 1482
TspRI	CASTG	2 cut(s)	118, 1570
Van91I	CCANNNNNTGG	1 cut(s)	1657
VpaK11BI	GGWCC	1 cut(s)	368
XapI	RAATTY	3 cut(s)	6, 1367, 1401
XceI	RCATGY	2 cut(s)	1119, 1200
XcmI	CCANNNNNNNNNTGG	1 cut(s)	303
XspI	CTAG	2 cut(s)	182, 1182
Zsp2I	ATGCAT	1 cut(s)	638

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.