MD17G1246500.v1.1

Belongs to the terpene cyclase mutase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0001409
Physical Location & Seq
Reverse (-)
29469470 .. 29470939
1470 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1246500.v1.1.491

Sequence Viewer

Length: 540 bp
ATGGGGGAAAATGTGGCTTTCAGTATGAACTCAGTGTTTTTCTTGTTGGATTCTACCAAGGGTGTAGCATATGAATGGTCTGGGAATAATCCACTTCCGCCTGAAGTGTGGCTATGTCCTTACTCTCTTCCATTTCATCCAGGAAGAATGTGGTGTCATTGCCGAATGGTGTATTTGCCCATGTCATATTTGTATGGGAAGAGGTTTGTTGGACCTATCACACCCACAATCCGGTCATTAGCTTTATACAATCTATACTATCCGCACCCAATGGTACAAGATATCTTGTGGGGGTCTCTTCACTATGTTTATGAACCTGTTTTCACACGTTGGCCAGCAAAAGAATTGAGGGAGAGTGCTTTGAAGACTGTAATGCAGCATATACATTATGAAGATGAAAATACTCGGTACTTATGCATAGGGCCTGTTAACAAGGTGTTAAATATGCTTTGTTGTTGGGCAGAGGATCCAAACTCAGAGGCATTCAATTGCATCTCCCGAGAATCCCTGATTATTTATGGATCGCTGAAGATGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00100 ko01100 ko01110 map00100 map01100 map01110
Pfam Domains
Protein Families
SQHop_cyclase_C SQHop_cyclase_N

Protein Analysis

180

Amino Acids

20.83

Weight (kDa)

6.58

Isoelectric Point (pI)

48.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SQHop_cyclase_N PF13249 24 - 142 3.8e-09 Squalene-hopene cyclase N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 98, 263
AclWI GGATC 3 cut(s) 461, 474, 529
AcoI YGGCCR 1 cut(s) 332
AcuI CTGAAG 1 cut(s) 123
AfaI GTAC 2 cut(s) 276, 410
AfiI CCNNNNNNNGG 1 cut(s) 231
AflIII ACRYGT 1 cut(s) 326
AgsI TTSAA 2 cut(s) 364, 487
AjnI CCWGG 1 cut(s) 139
AluBI AGCT 1 cut(s) 242
AluI AGCT 1 cut(s) 242
Alw26I GTCTC 1 cut(s) 300
AlwI GGATC 3 cut(s) 461, 474, 529
Ama87I CYCGRG 1 cut(s) 498
AoxI GGCC 2 cut(s) 332, 422
ApeKI GCWGC 1 cut(s) 376
AspS9I GGNCC 2 cut(s) 212, 422
AvaI CYCGRG 1 cut(s) 498
AvaII GGWCC 1 cut(s) 212
BalI TGGCCA 1 cut(s) 334
BamHI GGATCC 1 cut(s) 466
BbsI GAAGAC 1 cut(s) 371
BbvI GCAGC 1 cut(s) 388
BccI CCATC 1 cut(s) 526
BciT130I CCWGG 1 cut(s) 141
BcoDI GTCTC 1 cut(s) 300
BisI GCNGC 1 cut(s) 377
BlsI GCNGC 1 cut(s) 378
Bme1390I CCNGG 1 cut(s) 141
Bme18I GGWCC 1 cut(s) 212
BmeT110I CYCGRG 1 cut(s) 498
BmgT120I GGNCC 2 cut(s) 212, 422
BmiI GGNNCC 1 cut(s) 468
BmrFI CCNGG 1 cut(s) 141
BmsI GCATC 1 cut(s) 501
BpiI GAAGAC 1 cut(s) 371
BsaI GGTCTC 1 cut(s) 300
BsaJI CCNNGG 1 cut(s) 57
BsaWI WCCGGW 1 cut(s) 231
Bsc4I CCNNNNNNNGG 1 cut(s) 231
Bse3DI GCAATG 1 cut(s) 157
BseBI CCWGG 1 cut(s) 141
BseDI CCNNGG 1 cut(s) 57
BseGI GGATG 1 cut(s) 136
BseLI CCNNNNNNNGG 1 cut(s) 231
BseMI GCAATG 1 cut(s) 157
BseMII CTCAG 2 cut(s) 45, 489
BseXI GCAGC 1 cut(s) 388
BshFI GGCC 2 cut(s) 334, 424
BsiHKCI CYCGRG 1 cut(s) 498
BsiSI CCGG 1 cut(s) 232
BslI CCNNNNNNNGG 1 cut(s) 231
BsmAI GTCTC 1 cut(s) 300
BsmI GAATGC 1 cut(s) 482
BsnI GGCC 2 cut(s) 334, 424
Bso31I GGTCTC 1 cut(s) 300
BsoBI CYCGRG 1 cut(s) 498
Bsp143I GATC 2 cut(s) 466, 521
BspACI CCGC 2 cut(s) 98, 263
BspANI GGCC 2 cut(s) 334, 424
BspCNI CTCAG 2 cut(s) 44, 488
BspLI GGNNCC 1 cut(s) 468
BspPI GGATC 3 cut(s) 461, 474, 529
BspTNI GGTCTC 1 cut(s) 300
BsrDI GCAATG 1 cut(s) 157
BssECI CCNNGG 1 cut(s) 57
BssMI GATC 2 cut(s) 466, 521
BssT1I CCWWGG 1 cut(s) 57
Bst2UI CCWGG 1 cut(s) 141
Bst4CI ACNGT 1 cut(s) 370
Bst6I CTCTTC 3 cut(s) 132, 194, 303
BstC8I GCNNGC 1 cut(s) 336
BstDEI CTNAG 2 cut(s) 31, 475
BstF5I GGATG 1 cut(s) 136
BstKTI GATC 2 cut(s) 469, 524
BstMAI GTCTC 1 cut(s) 300
BstMBI GATC 2 cut(s) 466, 521
BstNI CCWGG 1 cut(s) 141
BstSCI CCNGG 1 cut(s) 139
BstV1I GCAGC 1 cut(s) 388
BstV2I GAAGAC 1 cut(s) 371
BstX2I RGATCY 1 cut(s) 466
BstYI RGATCY 1 cut(s) 466
BsuRI GGCC 2 cut(s) 334, 424
BtsCI GGATG 1 cut(s) 136
BtsIMutI CAGTG 1 cut(s) 39
Cac8I GCNNGC 1 cut(s) 336
Cfr13I GGNCC 2 cut(s) 212, 422
Csp6I GTAC 2 cut(s) 275, 409
CviAII CATG 2 cut(s) 181, 537
CviJI RGCY 5 cut(s) 17, 112, 242, 334, 424
CviKI_1 RGCY 5 cut(s) 17, 112, 242, 334, 424
CviQI GTAC 2 cut(s) 275, 409
DdeI CTNAG 2 cut(s) 31, 475
DpnI GATC 2 cut(s) 468, 523
DpnII GATC 2 cut(s) 466, 521
EaeI YGGCCR 1 cut(s) 332
Eam1104I CTCTTC 3 cut(s) 132, 194, 303
EarI CTCTTC 3 cut(s) 132, 194, 303
EciI GGCGGA 1 cut(s) 87
Eco130I CCWWGG 1 cut(s) 57
Eco31I GGTCTC 1 cut(s) 300
Eco32I GATATC 1 cut(s) 283
Eco47I GGWCC 1 cut(s) 212
Eco57I CTGAAG 1 cut(s) 123
Eco88I CYCGRG 1 cut(s) 498
EcoO109I RGGNCCY 1 cut(s) 422
EcoRII CCWGG 1 cut(s) 139
EcoRV GATATC 1 cut(s) 283
EcoT14I CCWWGG 1 cut(s) 57
EcoT22I ATGCAT 1 cut(s) 419
ErhI CCWWGG 1 cut(s) 57
FaeI CATG 2 cut(s) 184, 540
FatI CATG 2 cut(s) 180, 536
FauNDI CATATG 1 cut(s) 70
Fnu4HI GCNGC 1 cut(s) 377
FokI GGATG 1 cut(s) 123
Fsp4HI GCNGC 1 cut(s) 377
GluI GCNGC 1 cut(s) 377
HaeIII GGCC 2 cut(s) 334, 424
HapII CCGG 1 cut(s) 232
Hin1II CATG 2 cut(s) 184, 540
HincII GTYRAC 1 cut(s) 430
HindII GTYRAC 1 cut(s) 430
HinfI GANTC 2 cut(s) 50, 503
HpaI GTTAAC 1 cut(s) 430
HpaII CCGG 1 cut(s) 232
Hpy166II GTNNAC 1 cut(s) 430
Hpy188I TCNGA 1 cut(s) 478
Hpy188III TCNNGA 1 cut(s) 498
Hpy8I GTNNAC 1 cut(s) 430
HpyCH4III ACNGT 1 cut(s) 370
HpyCH4IV ACGT 1 cut(s) 328
HpyCH4V TGCA 3 cut(s) 376, 417, 492
HpyF3I CTNAG 2 cut(s) 31, 475
HpySE526I ACGT 1 cut(s) 328
Hsp92II CATG 2 cut(s) 184, 540
KspAI GTTAAC 1 cut(s) 430
Kzo9I GATC 2 cut(s) 466, 521
LpnPI CCDG 9 cut(s) 66, 114, 126, 153, 245, 330, 348, 438, 521
Lsp1109I GCAGC 1 cut(s) 388
LweI GCATC 1 cut(s) 501
MaeII ACGT 1 cut(s) 328
MalI GATC 2 cut(s) 468, 523
MboI GATC 2 cut(s) 466, 521
MboII GAAGA 6 cut(s) 119, 156, 211, 290, 376, 404
MfeI CAATTG 1 cut(s) 487
MflI RGATCY 1 cut(s) 466
MlsI TGGCCA 1 cut(s) 334
MluCI AATT 2 cut(s) 344, 487
MluNI TGGCCA 1 cut(s) 334
MmeI TCCRAC 2 cut(s) 27, 190
MnlI CCTC 4 cut(s) 195, 342, 457, 472
Mox20I TGGCCA 1 cut(s) 334
Mph1103I ATGCAT 1 cut(s) 419
MscI TGGCCA 1 cut(s) 334
MseI TTAA 2 cut(s) 429, 440
MslI CAYNNNNRTG 1 cut(s) 73
Msp20I TGGCCA 1 cut(s) 334
MspI CCGG 1 cut(s) 232
MspR9I CCNGG 1 cut(s) 141
MunI CAATTG 1 cut(s) 487
Mva1269I GAATGC 1 cut(s) 482
MvaI CCWGG 1 cut(s) 141
NdeI CATATG 1 cut(s) 70
NdeII GATC 2 cut(s) 466, 521
NlaIII CATG 2 cut(s) 184, 540
NlaIV GGNNCC 1 cut(s) 468
NsiI ATGCAT 1 cut(s) 419
PctI GAATGC 1 cut(s) 482
PfeI GAWTC 2 cut(s) 50, 503
PfoI TCCNGGA 1 cut(s) 139
PkrI GCNGC 1 cut(s) 378
Psp6I CCWGG 1 cut(s) 139
PspGI CCWGG 1 cut(s) 139
PspN4I GGNNCC 1 cut(s) 468
PspPI GGNCC 2 cut(s) 212, 422
PsuI RGATCY 1 cut(s) 466
RsaI GTAC 2 cut(s) 276, 410
RsaNI GTAC 2 cut(s) 275, 409
RseI CAYNNNNRTG 1 cut(s) 73
SaqAI TTAA 2 cut(s) 429, 440
SatI GCNGC 1 cut(s) 377
Sau3AI GATC 2 cut(s) 466, 521
Sau96I GGNCC 2 cut(s) 212, 422
ScrFI CCNGG 1 cut(s) 141
SetI ASST 6 cut(s) 206, 217, 244, 319, 331, 438
SfaNI GCATC 1 cut(s) 501
SinI GGWCC 1 cut(s) 212
SmiMI CAYNNNNRTG 1 cut(s) 73
Sse9I AATT 2 cut(s) 344, 487
SsiI CCGC 2 cut(s) 98, 263
StyD4I CCNGG 1 cut(s) 139
StyI CCWWGG 1 cut(s) 57
TaaI ACNGT 1 cut(s) 370
TaiI ACGT 1 cut(s) 331
TasI AATT 2 cut(s) 344, 487
TfiI GAWTC 2 cut(s) 50, 503
Tru1I TTAA 2 cut(s) 429, 440
Tru9I TTAA 2 cut(s) 429, 440
TscAI CASTG 1 cut(s) 39
TseI GCWGC 1 cut(s) 376
TspDTI ATGAA 6 cut(s) 41, 87, 125, 327, 405, 411
TspRI CASTG 1 cut(s) 39
VpaK11BI GGWCC 1 cut(s) 212
XcmI CCANNNNNNNNNTGG 1 cut(s) 147
Zsp2I ATGCAT 1 cut(s) 419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.