MD17G1246000.v1.1

Belongs to the terpene cyclase mutase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0001409
Physical Location & Seq
Reverse (-)
29439182 .. 29440820
1639 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1246000.v1.1.491

Sequence Viewer

Length: 618 bp
ATGTTTCTGCTTCCTGGTTTGATCATTACACTTTCAATTACTGGAGCTCCAAATGCTGTGTTATTGAAAGAGCATCAACGTGAGATGTGCAGATATCTCTATAATCATCAGAATGAAGATGGGGGATGGGGTCTGCATATTGAGGGGCCAAGTACCATGTTTGGCACTGCACTTAATTATGTCACTTTGAGGTTGCTTGGTGAAGGTGCTGATGATGGACAGGGAGCAATCGAACTAGCACGCAAGTGGATATTAGACCATGGTGGCGTGACTGCAGTTACTTCATGGGGGAAAATGTGGCTTTCAGTACTTGGGGCATATGAATGGTCTGGGAATAATCCACTTCCGCCTGAAGTGTGGCTATGTCCTTACTCTCTTCCATTTCATCCAGGAAGAATGTGGTGTCATTGCCGAATGGTATATTTGCCCATGTCATATTTGTATGGGAAGAGGTTTGTTGGACCTATCACACCCACAATCCGGTCATTGAGGAAGGAGCTTTATACAGTCCCCTATCATGAAGTTGATTGGAATAATGCTCGCAATCTACATGCAGAGGAAGATCTATACCATCAGCACCCAATGGTACAAGATATCTTGTGGGGGTCTCTTCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00100 ko01100 ko01110 map00100 map01100 map01110
Pfam Domains
Protein Families
SQHop_cyclase_C SQHop_cyclase_N

Protein Analysis

206

Amino Acids

23.44

Weight (kDa)

6.24

Isoelectric Point (pI)

36.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SQHop_cyclase_N PF13249 23 - 179 1e-34 Squalene-hopene cyclase N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 347
AcuI CTGAAG 1 cut(s) 372
AfaI GTAC 3 cut(s) 154, 309, 588
AfiI CCNNNNNNNGG 1 cut(s) 480
AgsI TTSAA 2 cut(s) 36, 67
AjnI CCWGG 2 cut(s) 13, 388
AleI CACNNNNGTG 1 cut(s) 244
AluBI AGCT 2 cut(s) 47, 499
AluI AGCT 2 cut(s) 47, 499
Alw21I GWGCWC 1 cut(s) 49
Alw26I GTCTC 1 cut(s) 612
AoxI GGCC 1 cut(s) 146
AspS9I GGNCC 2 cut(s) 146, 461
AsuHPI GGTGA 1 cut(s) 212
AvaII GGWCC 1 cut(s) 461
BanII GRGCYC 1 cut(s) 49
Bbv12I GWGCWC 1 cut(s) 49
BccI CCATC 4 cut(s) 113, 120, 209, 579
BciT130I CCWGG 2 cut(s) 15, 390
BclI TGATCA 1 cut(s) 21
BcoDI GTCTC 1 cut(s) 612
BfaI CTAG 1 cut(s) 236
BfmI CTRYAG 1 cut(s) 273
BglII AGATCT 1 cut(s) 562
BmcAI AGTACT 1 cut(s) 309
Bme1390I CCNGG 2 cut(s) 15, 390
Bme18I GGWCC 1 cut(s) 461
BmgT120I GGNCC 2 cut(s) 146, 461
BmiI GGNNCC 1 cut(s) 147
BmrFI CCNGG 2 cut(s) 15, 390
BmsI GCATC 1 cut(s) 82
BpmI CTGGAG 1 cut(s) 63
BsaI GGTCTC 1 cut(s) 612
BsaJI CCNNGG 1 cut(s) 259
BsaWI WCCGGW 1 cut(s) 480
BsaXI ACNNNNNCTCC 2 cut(s) 31, 61
Bsc4I CCNNNNNNNGG 1 cut(s) 480
Bse1I ACTGG 1 cut(s) 46
Bse3DI GCAATG 1 cut(s) 406
BseBI CCWGG 2 cut(s) 15, 390
BseDI CCNNGG 1 cut(s) 259
BseGI GGATG 2 cut(s) 131, 385
BseLI CCNNNNNNNGG 1 cut(s) 480
BseMI GCAATG 1 cut(s) 406
BseNI ACTGG 1 cut(s) 46
BsgI GTGCAG 2 cut(s) 109, 153
BshFI GGCC 1 cut(s) 148
BsiHKAI GWGCWC 1 cut(s) 49
BsiSI CCGG 1 cut(s) 481
BslFI GGGAC 1 cut(s) 494
BslI CCNNNNNNNGG 1 cut(s) 480
BsmAI GTCTC 1 cut(s) 612
BsmFI GGGAC 1 cut(s) 494
BsnI GGCC 1 cut(s) 148
Bso31I GGTCTC 1 cut(s) 612
Bsp1286I GDGCHC 1 cut(s) 49
Bsp143I GATC 2 cut(s) 21, 562
Bsp19I CCATGG 1 cut(s) 259
BspACI CCGC 1 cut(s) 347
BspANI GGCC 1 cut(s) 148
BspHI TCATGA 1 cut(s) 517
BspLI GGNNCC 1 cut(s) 147
BspMAI CTGCAG 1 cut(s) 277
BspTNI GGTCTC 1 cut(s) 612
BsrDI GCAATG 1 cut(s) 406
BsrI ACTGG 1 cut(s) 46
BssECI CCNNGG 1 cut(s) 259
BssMI GATC 2 cut(s) 21, 562
BssT1I CCWWGG 1 cut(s) 259
Bst2UI CCWGG 2 cut(s) 15, 390
Bst4CI ACNGT 1 cut(s) 508
Bst6I CTCTTC 3 cut(s) 381, 443, 615
BstC8I GCNNGC 2 cut(s) 241, 541
BstDSI CCRYGG 1 cut(s) 259
BstF5I GGATG 2 cut(s) 131, 385
BstKTI GATC 2 cut(s) 24, 565
BstMAI GTCTC 1 cut(s) 612
BstMBI GATC 2 cut(s) 21, 562
BstMWI GCNNNNNNNGC 1 cut(s) 53
BstNI CCWGG 2 cut(s) 15, 390
BstNSI RCATGY 1 cut(s) 554
BstSCI CCNGG 2 cut(s) 13, 388
BstSFI CTRYAG 1 cut(s) 273
BstX2I RGATCY 1 cut(s) 562
BstYI RGATCY 1 cut(s) 562
BsuRI GGCC 1 cut(s) 148
BtgI CCRYGG 1 cut(s) 259
BtsCI GGATG 2 cut(s) 131, 385
BtsI GCAGTG 1 cut(s) 165
BtsIMutI CAGTG 1 cut(s) 165
Cac8I GCNNGC 2 cut(s) 241, 541
CciI TCATGA 1 cut(s) 517
Cfr13I GGNCC 2 cut(s) 146, 461
Csp6I GTAC 3 cut(s) 153, 308, 587
CviAII CATG 6 cut(s) 157, 260, 285, 430, 518, 551
CviJI RGCY 5 cut(s) 47, 148, 301, 361, 499
CviKI_1 RGCY 5 cut(s) 47, 148, 301, 361, 499
CviQI GTAC 3 cut(s) 153, 308, 587
DpnI GATC 2 cut(s) 23, 564
DpnII GATC 2 cut(s) 21, 562
Eam1104I CTCTTC 3 cut(s) 381, 443, 615
EarI CTCTTC 3 cut(s) 381, 443, 615
EciI GGCGGA 1 cut(s) 336
Ecl136II GAGCTC 1 cut(s) 47
Eco130I CCWWGG 1 cut(s) 259
Eco24I GRGCYC 1 cut(s) 49
Eco31I GGTCTC 1 cut(s) 612
Eco32I GATATC 2 cut(s) 95, 595
Eco47I GGWCC 1 cut(s) 461
Eco53kI GAGCTC 1 cut(s) 47
Eco57I CTGAAG 1 cut(s) 372
EcoICRI GAGCTC 1 cut(s) 47
EcoRII CCWGG 2 cut(s) 13, 388
EcoRV GATATC 2 cut(s) 95, 595
EcoT14I CCWWGG 1 cut(s) 259
EcoT38I GRGCYC 1 cut(s) 49
ErhI CCWWGG 1 cut(s) 259
FaeI CATG 6 cut(s) 160, 263, 288, 433, 521, 554
FaqI GGGAC 1 cut(s) 494
FatI CATG 6 cut(s) 156, 259, 284, 429, 517, 550
FauNDI CATATG 1 cut(s) 319
FbaI TGATCA 1 cut(s) 21
FokI GGATG 2 cut(s) 138, 372
FriOI GRGCYC 1 cut(s) 49
FspBI CTAG 1 cut(s) 236
GsuI CTGGAG 1 cut(s) 63
HaeIII GGCC 1 cut(s) 148
HapII CCGG 1 cut(s) 481
Hin1II CATG 6 cut(s) 160, 263, 288, 433, 521, 554
HpaII CCGG 1 cut(s) 481
HphI GGTGA 1 cut(s) 212
Hpy188I TCNGA 1 cut(s) 111
Hpy188III TCNNGA 1 cut(s) 518
HpyAV CCTTC 2 cut(s) 197, 487
HpyCH4III ACNGT 1 cut(s) 508
HpyCH4IV ACGT 1 cut(s) 79
HpyCH4V TGCA 5 cut(s) 90, 136, 170, 275, 554
HpyF10VI GCNNNNNNNGC 1 cut(s) 53
HpySE526I ACGT 1 cut(s) 79
Hsp92II CATG 6 cut(s) 160, 263, 288, 433, 521, 554
Ksp22I TGATCA 1 cut(s) 21
Kzo9I GATC 2 cut(s) 21, 562
LmnI GCTCC 4 cut(s) 44, 52, 224, 496
LpnPI CCDG 8 cut(s) 27, 27, 206, 315, 363, 375, 402, 494
LweI GCATC 1 cut(s) 82
MaeI CTAG 1 cut(s) 236
MaeII ACGT 1 cut(s) 79
MaeIII GTNAC 3 cut(s) 181, 268, 277
MalI GATC 2 cut(s) 23, 564
MboI GATC 2 cut(s) 21, 562
MboII GAAGA 6 cut(s) 128, 368, 405, 460, 572, 602
MflI RGATCY 1 cut(s) 562
MhlI GDGCHC 1 cut(s) 49
MluCI AATT 2 cut(s) 36, 175
MmeI TCCRAC 1 cut(s) 439
MnlI CCTC 5 cut(s) 136, 183, 444, 483, 550
MseI TTAA 1 cut(s) 174
MslI CAYNNNNRTG 4 cut(s) 78, 111, 244, 322
MspI CCGG 1 cut(s) 481
MspR9I CCNGG 2 cut(s) 15, 390
MvaI CCWGG 2 cut(s) 15, 390
MwoI GCNNNNNNNGC 1 cut(s) 53
NcoI CCATGG 1 cut(s) 259
NdeI CATATG 1 cut(s) 319
NdeII GATC 2 cut(s) 21, 562
NlaIII CATG 6 cut(s) 160, 263, 288, 433, 521, 554
NlaIV GGNNCC 1 cut(s) 147
NmuCI GTSAC 2 cut(s) 181, 268
NspI RCATGY 1 cut(s) 554
OliI CACNNNNGTG 1 cut(s) 244
PagI TCATGA 1 cut(s) 517
PfoI TCCNGGA 1 cut(s) 388
Psp124BI GAGCTC 1 cut(s) 49
Psp6I CCWGG 2 cut(s) 13, 388
PspGI CCWGG 2 cut(s) 13, 388
PspN4I GGNNCC 1 cut(s) 147
PspPI GGNCC 2 cut(s) 146, 461
PstI CTGCAG 1 cut(s) 277
PsuI RGATCY 1 cut(s) 562
RsaI GTAC 3 cut(s) 154, 309, 588
RsaNI GTAC 3 cut(s) 153, 308, 587
RseI CAYNNNNRTG 4 cut(s) 78, 111, 244, 322
SacI GAGCTC 1 cut(s) 49
SaqAI TTAA 1 cut(s) 174
Sau3AI GATC 2 cut(s) 21, 562
Sau96I GGNCC 2 cut(s) 146, 461
ScaI AGTACT 1 cut(s) 309
ScrFI CCNGG 2 cut(s) 15, 390
SduI GDGCHC 1 cut(s) 49
SetI ASST 7 cut(s) 49, 82, 194, 208, 455, 466, 501
SfaNI GCATC 1 cut(s) 82
SfcI CTRYAG 1 cut(s) 273
SinI GGWCC 1 cut(s) 461
SmiMI CAYNNNNRTG 4 cut(s) 78, 111, 244, 322
Sse9I AATT 2 cut(s) 36, 175
SsiI CCGC 1 cut(s) 347
SspMI CTAG 1 cut(s) 236
SstI GAGCTC 1 cut(s) 49
StyD4I CCNGG 2 cut(s) 13, 388
StyI CCWWGG 1 cut(s) 259
TaaI ACNGT 1 cut(s) 508
TaiI ACGT 1 cut(s) 82
TaqI TCGA 1 cut(s) 231
TasI AATT 2 cut(s) 36, 175
TatI WGTACW 1 cut(s) 307
Tru1I TTAA 1 cut(s) 174
Tru9I TTAA 1 cut(s) 174
TscAI CASTG 1 cut(s) 172
TseFI GTSAC 2 cut(s) 181, 268
Tsp45I GTSAC 2 cut(s) 181, 268
TspDTI ATGAA 5 cut(s) 129, 273, 336, 374, 534
TspRI CASTG 1 cut(s) 172
VpaK11BI GGWCC 1 cut(s) 461
XceI RCATGY 1 cut(s) 554
XcmI CCANNNNNNNNNTGG 1 cut(s) 396
XspI CTAG 1 cut(s) 236
ZrmI AGTACT 1 cut(s) 309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.