Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000005

Orthogroup ID

OG0001409

Physical Location & Seq

Genomic Coordinates Forward (+)

458590 .. 463532

Gene Structure 4943 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_5G00333010.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2172 bp

ATGTGGAAGCTGAAGATTGCAGAGGGAGGACCCTGGCTGAGGACACTAAACAATCACGTGGGTCGACAACACTGGGAATTTGATCCAGATGCCGGAACACCAGAAGAACGAGCCGAAGTAGAGAGAGTTCGTGAGGAGTTTACGAGAAACCGCTTTCGGATCAAACAAAGTGCTGATCTTTTGATGAGAATGCAGCTTACTAAGGAAAACCCTAGTGGACCAATTCCGGCAGCAATTAAAGTGAAGGGAATAGATGATATAACAGAGAAGGCAGTGACAGCAACAATAAGAAGGTCTCTCAGCTTCTATTCCTCCCTCCAGGCCCATGATGGCCATTGGCCTGCTGAATCTGCCGGTCCCTTGTTCTTCATTCAACCTTTGGTTATAGCGTTGTATGTTACTGGAGCATTGTGTGCCGTTCTAGGACCTGAACACCAGAAGGAAATTATTCGTTACATCTACAATCATCAGAATGAAGATGGAGGTTGGGGACTACACATAGAAGGTCATAGCACGATGTTTGGGTCAGCGCTAAGCTACATTGCGTTGAGGCTGCTAGGAGAGGGTCCTGATGATGGGGAAGGCATGGCCGTGGCAAGAGCCAGGAAATGGATTCTTGATCATGGTGGTGCGGTTGGAATTCCATCTTGGGGAAAGTTTTGGCTTACGGGTGCAACCCGTTGCCCCCCTGAGTTCTGGCTTCTACCAAATATGTCCCCTATTCATCCAGGGAAGATGTTATGCTATTGTCGGTTGGTGTACATGCCTATGTCATACTTATACGGGAAGAGGTTTGTTGGCCAAATCACCGGATTGGTTCGATCACTAAGACGAGAGCTCTACACTGAGCCATACTTTGACATTAACTGGACTAAAGCCAGAAACACTGTTGCAAAGGAAGATTTGTATTATCCACACCCCCTGGTGCAAGATATGCTGTGGGGATTTCTTCACCATGTTGCTGAACCAATTCTGGCTCGTTGGCCCTTTTCTATCCTGAGAAAGAAGGCCCTAGAAGTAGCAATCGAGCACGTACACTATGAAGACGAGAACAGCAGATATCTTTGCATTGGAAGTGTAGAAAAGGTGTTATGTTTGATTGCTTGTTGGGTCGAAGATCCAAATTCGGAAGCGTACAAGCTTCACCTGGCTAGAATACCAGATTACTTTTGGATCGCTGAAGATGGCTTGAAATTTCAGAGTTTCGGTAGTCAAATGTGGGACGCAGCCTTTGCAATTCAGGCAATACTCTCTTGTGACCCAAGTGAAGAGTATGGGACAACACTTCGAAAAGCGCACGACTTTGTGAAGGCTTCACAGGACCATGGTTGGCAAGTCTCCGACTGCACTTCAGAAGGACTAAAGGTTGCACTCTTGCTCTCGCAAATGCCAGAGAAACTAGTTGGGGAAGGAATGGAAAGAGAATGTTTTTATGATGCTGTGAATGTCATTCTTTCTTTACAAAGCATCAGTGGCGGTTTCCCTGCTTGGGAGCCTCAAAGAGCATTTCGTTGGTTGGAGAAATTCAACCCCACAGAGTTCTTTGAAGATACTCTTATTGAACGAGAGTACGTAGAATGCACCGCATCCGCAGTTCAAGGTCTAGCACTCTTTAGAAGATTGTATCCCAACCATCGAAGAAAGGAAATAGACAGTTGCATATCAAGGGCAATTCGTTACATTGAAGAAGCACAAAACCCTGATGGTTCATGGTACGGATGCTGGGGTATTTGCTACACATATGGTACCTGGTTTGGTGTAGGAGCACTAGCATCCTGTGGGAAGAAGTACCAGAATAGTCCCATATTGCGCAAAGCCTGTGCATTTTTGCTATCAAAGCAGCTACCCAATGGTGGATGGGGAGAGAGCTACCTTTCCAGTCAAAATAAGGTTTATACGAATCTAGAAGGCAACCGCGCTAATCTGGTACACACTTCATGGGCTTTATTGTCCCTCATCGATGCTGGGCAGGGTGATGTAGATCCAACCCCCATTCATCGTGGAATAAGGGTTTTGATCAATTCACAAATGGAGGATGGTGACTTCCCTCAACAGGAAATTACTGGAGTATTCATGAGAAATTGTACACTAAACTACTCCTCATATCGAAATATCTTCCCGAAATGGGCTCTCGGGGAGTACCGCCGACGCATTCTGATGCTTAAATCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0006629 GO:0006720 GO:0006721 GO:0006722 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009987 GO:0016104 GO:0016114 GO:0016853 GO:0016866 GO:0019742 GO:0019745 GO:0031559 GO:0042299 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0071704 GO:1901360 GO:1901362 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00909 ko01110 map00909 map01110

Pfam Domains

Protein Families

SQHop_cyclase_C SQHop_cyclase_N

Protein Analysis

723

Amino Acids

82.46

Weight (kDa)

6.51

Isoelectric Point (pI)

50.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SQHop_cyclase_N	PF13249	96 - 351	5.1e-29	Squalene-hopene cyclase N-terminal domain
SQHop_cyclase_C	PF13243	472 - 716	6.3e-44	Squalene-hopene cyclase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001409)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g10860 FvH4_7g10860 FvH4_7g10860
malus_domestica	MD17G1158900.v1.1 MD17G1160000.v1.1 MD17G1246000.v1.1 MD17G1246100.v1.1 MD17G1246500.v1.1 MD17G1246700.v1.1
prunus_persica	Prupe.1G002600_v2.0.a1 Prupe.1G002600_v2.0.a1 Prupe.7G043400_v2.0.a1
pyrus_communis	pycom16g19870 pycom16g24410
rosa_chinensis	RchiOBHm_Chr1g0332711 RchiOBHm_Chr2g0148781 RchiOBHm_Chr4g0385071 RchiOBHm_Chr4g0385101
rosa_laevigata	RLG00000010257
rosa_multiflora	Rmu_co8015556.1_g000001 Rmu_co8308753.1_g000001 Rmu_co8434809.1_g000001 Rmu_sc0000824.1_g000004 Rmu_sc0001548.1_g000031 Rmu_sc0001548.1_g000053 Rmu_sc0002070.1_g000040 Rmu_sc0002116.1_g000014 Rmu_sc0003929.1_g000004 Rmu_sc0008546.1_g000012 Rmu_sc0008678.1_g000005 Rmu_ssc0000158.1_g000036
rosa_roxburghii	Rroxscaffold_5G00333010 Rroxscaffold_5G00342310
rosa_rugosa	Rorug03G0113700 Rorug03G0225800 Rorug03G0298900
rosa_samantha	Rh1CG121300 Rh1DG132500 Rh2AG466900 Rh2CG453300 Rh2CG453700 Rh4AG002600 Rh4AG078200 Rh4AG078500 Rh4BG002100 Rh4BG073600 Rh4BG073800 Rh4BG075600 Rh4BG076300 Rh4DG002100 Rh4DG071700 Rh7BG219000
rosa_wichuraiana	Rw4G000670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1811
Acc65I	GGTACC	1 cut(s)	1745
AccB1I	GGYRCC	1 cut(s)	1745
AccB7I	CCANNNNNTGG	1 cut(s)	609
AccI	GTMKAC	1 cut(s)	64
AccII	CGCG	1 cut(s)	1917
AciI	CCGC	7 cut(s)	151, 632, 1476, 1584, 1590, 1915, 2143
AclWI	GGATC	5 cut(s)	77, 167, 1112, 1181, 1976
AcoI	YGGCCR	3 cut(s)	331, 588, 799
AcsI	RAATTY	5 cut(s)	77, 639, 1123, 1193, 1523
AcuI	CTGAAG	3 cut(s)	32, 1200, 1335
AcvI	CACGTG	1 cut(s)	58
AfeI	AGCGCT	1 cut(s)	531
AfiI	CCNNNNNNNGG	9 cut(s)	39, 92, 575, 609, 650, 1489, 1853, 2053, 2125
AgsI	TTSAA	7 cut(s)	374, 1192, 1528, 1547, 1562, 1598, 1685
AhlI	ACTAGT	1 cut(s)	1399
AjnI	CCWGG	7 cut(s)	32, 318, 602, 727, 921, 1146, 1748
AjuI	GAANNNNNNNTTGG	2 cut(s)	631, 663
AluBI	AGCT	8 cut(s)	10, 196, 303, 537, 838, 1141, 1843, 1869
AluI	AGCT	8 cut(s)	10, 196, 303, 537, 838, 1141, 1843, 1869
Alw21I	GWGCWC	3 cut(s)	840, 1032, 1768
Alw26I	GTCTC	2 cut(s)	300, 1342
AlwI	GGATC	5 cut(s)	77, 167, 1112, 1181, 1976
Ama87I	CYCGRG	1 cut(s)	2132
Aor51HI	AGCGCT	1 cut(s)	531
AoxI	GGCC	7 cut(s)	321, 331, 338, 588, 799, 983, 1008
ApeKI	GCWGC	5 cut(s)	193, 230, 553, 1226, 1840
ApoI	RAATTY	5 cut(s)	77, 639, 1123, 1193, 1523
ArsI	GACNNNNNNTTYG	2 cut(s)	1214, 1246
Asp700I	GAANNNNTTC	2 cut(s)	447, 969
Asp718I	GGTACC	1 cut(s)	1745
AspLEI	GCGC	4 cut(s)	532, 1297, 1812, 1919
AspS9I	GGNCC	9 cut(s)	29, 218, 322, 356, 425, 566, 984, 1009, 1321
AsuHPI	GGTGA	5 cut(s)	799, 944, 1136, 1985, 2051
AsuII	TTCGAA	1 cut(s)	1288
AvaI	CYCGRG	1 cut(s)	2132
AvaII	GGWCC	6 cut(s)	29, 218, 356, 425, 566, 1321
BalI	TGGCCA	2 cut(s)	333, 801
BanI	GGYRCC	1 cut(s)	1745
BanII	GRGCYC	2 cut(s)	840, 2131
BbrPI	CACGTG	1 cut(s)	58
BbsI	GAAGAC	1 cut(s)	1050
Bbv12I	GWGCWC	3 cut(s)	840, 1032, 1768
BbvCI	CCTCAGC	1 cut(s)	38
BbvI	GCAGC	5 cut(s)	205, 242, 540, 1238, 1852
BccI	CCATC	9 cut(s)	323, 473, 569, 652, 1178, 1641, 1697, 1851, 2030
BceAI	ACGGC	2 cut(s)	401, 575
BciT130I	CCWGG	7 cut(s)	34, 320, 604, 729, 923, 1148, 1750
BciVI	GTATCC	1 cut(s)	1635
BclI	TGATCA	2 cut(s)	619, 2016
BcoDI	GTCTC	2 cut(s)	300, 1342
BcuI	ACTAGT	1 cut(s)	1399
BfaI	CTAG	9 cut(s)	213, 422, 557, 1013, 1152, 1400, 1604, 1769, 1904
BfoI	RGCGCY	1 cut(s)	533
BfuI	GTATCC	1 cut(s)	1635
BisI	GCNGC	5 cut(s)	194, 231, 554, 1227, 1841
BlpI	GCTNAGC	1 cut(s)	533
BlsI	GCNGC	5 cut(s)	195, 232, 555, 1228, 1842
Bme1390I	CCNGG	7 cut(s)	34, 320, 604, 729, 923, 1148, 1750
Bme18I	GGWCC	6 cut(s)	29, 218, 356, 425, 566, 1321
BmeT110I	CYCGRG	1 cut(s)	2132
BmgT120I	GGNCC	9 cut(s)	29, 218, 322, 356, 425, 566, 984, 1009, 1321
BmiI	GGNNCC	5 cut(s)	31, 358, 567, 1494, 1747
BmrFI	CCNGG	7 cut(s)	34, 320, 604, 729, 923, 1148, 1750
BmrI	ACTGGG	1 cut(s)	82
BmsI	GCATC	8 cut(s)	79, 1426, 1476, 1595, 1709, 1781, 1951, 2148
BmuI	ACTGGG	1 cut(s)	82
BpiI	GAAGAC	1 cut(s)	1050
BpmI	CTGGAG	3 cut(s)	302, 423, 2085
Bpu10I	CCTNAGC	1 cut(s)	38
Bpu1102I	GCTNAGC	1 cut(s)	533
Bpu14I	TTCGAA	1 cut(s)	1288
Bsa29I	ATCGAT	1 cut(s)	1959
BsaAI	YACGTR	3 cut(s)	58, 1033, 1573
BsaBI	GATNNNNATC	1 cut(s)	1980
BsaI	GGTCTC	1 cut(s)	300
BsaJI	CCNNGG	5 cut(s)	32, 591, 728, 921, 1324
BsaWI	WCCGGW	1 cut(s)	809
Bsc4I	CCNNNNNNNGG	9 cut(s)	39, 92, 575, 609, 650, 1489, 1853, 2053, 2125
Bse118I	RCCGGY	1 cut(s)	353
Bse1I	ACTGG	5 cut(s)	77, 406, 872, 1878, 2068
Bse3DI	GCAATG	1 cut(s)	540
Bse8I	GATNNNNATC	1 cut(s)	1980
BseBI	CCWGG	7 cut(s)	34, 320, 604, 729, 923, 1148, 1750
BseCI	ATCGAT	1 cut(s)	1959
BseDI	CCNNGG	5 cut(s)	32, 591, 728, 921, 1324
BseGI	GGATG	6 cut(s)	724, 1586, 1724, 1772, 1862, 2041
BseJI	GATNNNNATC	1 cut(s)	1980
BseLI	CCNNNNNNNGG	9 cut(s)	39, 92, 575, 609, 650, 1489, 1853, 2053, 2125
BseMI	GCAATG	1 cut(s)	540
BseMII	CTCAG	5 cut(s)	29, 313, 681, 837, 989
BseNI	ACTGG	5 cut(s)	77, 406, 872, 1878, 2068
BseRI	GAGGAG	2 cut(s)	149, 2089
BseXI	GCAGC	5 cut(s)	205, 242, 540, 1238, 1852
BseYI	CCCAGC	2 cut(s)	1722, 1964
BsgI	GTGCAG	1 cut(s)	1330
Bsh1236I	CGCG	1 cut(s)	1917
BshFI	GGCC	7 cut(s)	323, 333, 340, 590, 801, 985, 1010
BshNI	GGYRCC	1 cut(s)	1745
BshVI	ATCGAT	1 cut(s)	1959
BsiHKAI	GWGCWC	3 cut(s)	840, 1032, 1768
BsiHKCI	CYCGRG	1 cut(s)	2132
BsiSI	CCGG	4 cut(s)	93, 227, 354, 810
BslFI	GGGAC	7 cut(s)	342, 504, 700, 1235, 1291, 1785, 1936
BslI	CCNNNNNNNGG	9 cut(s)	39, 92, 575, 609, 650, 1489, 1853, 2053, 2125
BsmAI	GTCTC	2 cut(s)	300, 1342
BsmFI	GGGAC	7 cut(s)	342, 504, 700, 1235, 1291, 1785, 1936
BsmI	GAATGC	3 cut(s)	195, 1583, 2151
BsnI	GGCC	7 cut(s)	323, 333, 340, 590, 801, 985, 1010
Bso31I	GGTCTC	1 cut(s)	300
BsoBI	CYCGRG	1 cut(s)	2132
Bsp119I	TTCGAA	1 cut(s)	1288
Bsp1286I	GDGCHC	4 cut(s)	840, 1032, 1768, 2131
Bsp1407I	TGTACA	2 cut(s)	759, 2084
Bsp143I	GATC	9 cut(s)	82, 159, 175, 619, 821, 1117, 1173, 1981, 2016
Bsp1720I	GCTNAGC	1 cut(s)	533
Bsp19I	CCATGG	1 cut(s)	1324
BspACI	CCGC	7 cut(s)	151, 632, 1476, 1584, 1590, 1915, 2143
BspANI	GGCC	7 cut(s)	323, 333, 340, 590, 801, 985, 1010
BspCNI	CTCAG	5 cut(s)	30, 312, 682, 838, 990
BspDI	ATCGAT	1 cut(s)	1959
BspFNI	CGCG	1 cut(s)	1917
BspHI	TCATGA	1 cut(s)	2073
BspLI	GGNNCC	5 cut(s)	31, 358, 567, 1494, 1747
BspPI	GGATC	5 cut(s)	77, 167, 1112, 1181, 1976
BspT104I	TTCGAA	1 cut(s)	1288
BspT107I	GGYRCC	1 cut(s)	1745
BspTNI	GGTCTC	1 cut(s)	300
BsrDI	GCAATG	1 cut(s)	540
BsrFI	RCCGGY	1 cut(s)	353
BsrGI	TGTACA	2 cut(s)	759, 2084
BsrI	ACTGG	5 cut(s)	77, 406, 872, 1878, 2068
BssAI	RCCGGY	1 cut(s)	353
BssECI	CCNNGG	5 cut(s)	32, 591, 728, 921, 1324
BssMI	GATC	9 cut(s)	82, 159, 175, 619, 821, 1117, 1173, 1981, 2016
BssT1I	CCWWGG	1 cut(s)	1324
Bst2UI	CCWGG	7 cut(s)	34, 320, 604, 729, 923, 1148, 1750
Bst4CI	ACNGT	2 cut(s)	889, 1655
Bst6I	CTCTTC	2 cut(s)	782, 1263
BstAPI	GCANNNNNTGC	3 cut(s)	413, 934, 1232
BstAUI	TGTACA	2 cut(s)	759, 2084
BstBAI	YACGTR	3 cut(s)	58, 1033, 1573
BstBI	TTCGAA	1 cut(s)	1288
BstC8I	GCNNGC	1 cut(s)	342
BstDEI	CTNAG	8 cut(s)	38, 201, 299, 533, 690, 827, 846, 998
BstDSI	CCRYGG	2 cut(s)	591, 1324
BstENI	CCTNNNNNAGG	1 cut(s)	37
BstF5I	GGATG	6 cut(s)	724, 1586, 1724, 1772, 1862, 2041
BstFNI	CGCG	1 cut(s)	1917
BstH2I	RGCGCY	1 cut(s)	533
BstHHI	GCGC	4 cut(s)	532, 1297, 1812, 1919
BstKTI	GATC	9 cut(s)	85, 162, 178, 622, 824, 1120, 1176, 1984, 2019
BstMAI	GTCTC	2 cut(s)	300, 1342
BstMBI	GATC	9 cut(s)	82, 159, 175, 619, 821, 1117, 1173, 1981, 2016
BstMWI	GCNNNNNNNGC	8 cut(s)	278, 350, 413, 934, 1232, 1241, 1473, 1837
BstNI	CCWGG	7 cut(s)	34, 320, 604, 729, 923, 1148, 1750
BstNSI	RCATGY	1 cut(s)	766
BstSCI	CCNGG	7 cut(s)	32, 318, 602, 727, 921, 1146, 1748
BstSNI	TACGTA	1 cut(s)	1573
BstUI	CGCG	1 cut(s)	1917
BstV1I	GCAGC	5 cut(s)	205, 242, 540, 1238, 1852
BstV2I	GAAGAC	1 cut(s)	1050
BstX2I	RGATCY	2 cut(s)	1117, 1981
BstYI	RGATCY	2 cut(s)	1117, 1981
Bsu15I	ATCGAT	1 cut(s)	1959
BsuI	GTATCC	1 cut(s)	1635
BsuRI	GGCC	7 cut(s)	323, 333, 340, 590, 801, 985, 1010
BsuTUI	ATCGAT	1 cut(s)	1959
BtgI	CCRYGG	2 cut(s)	591, 1324
BtsCI	GGATG	6 cut(s)	724, 1586, 1724, 1772, 1862, 2041
BtsI	GCAGTG	1 cut(s)	279
BtsIMutI	CAGTG	5 cut(s)	70, 279, 843, 885, 1477
Cac8I	GCNNGC	1 cut(s)	342
CciI	TCATGA	1 cut(s)	2073
CfoI	GCGC	4 cut(s)	532, 1297, 1812, 1919
Cfr10I	RCCGGY	1 cut(s)	353
Cfr13I	GGNCC	9 cut(s)	29, 218, 322, 356, 425, 566, 984, 1009, 1321
ClaI	ATCGAT	1 cut(s)	1959
CseI	GACGC	2 cut(s)	1232, 2157
CsiI	ACCWGGT	1 cut(s)	1748
CviAII	CATG	9 cut(s)	326, 586, 623, 763, 956, 1325, 1710, 1938, 2074
DdeI	CTNAG	8 cut(s)	38, 201, 299, 533, 690, 827, 846, 998
DpnI	GATC	9 cut(s)	84, 161, 177, 621, 823, 1119, 1175, 1983, 2018
DpnII	GATC	9 cut(s)	82, 159, 175, 619, 821, 1117, 1173, 1981, 2016
EaeI	YGGCCR	3 cut(s)	331, 588, 799
Eam1104I	CTCTTC	2 cut(s)	782, 1263
EarI	CTCTTC	2 cut(s)	782, 1263
Ecl136II	GAGCTC	1 cut(s)	838
Eco105I	TACGTA	1 cut(s)	1573
Eco130I	CCWWGG	1 cut(s)	1324
Eco24I	GRGCYC	2 cut(s)	840, 2131
Eco31I	GGTCTC	1 cut(s)	300
Eco32I	GATATC	1 cut(s)	1061
Eco47I	GGWCC	6 cut(s)	29, 218, 356, 425, 566, 1321
Eco47III	AGCGCT	1 cut(s)	531
Eco53kI	GAGCTC	1 cut(s)	838
Eco57I	CTGAAG	3 cut(s)	32, 1200, 1335
Eco72I	CACGTG	1 cut(s)	58
Eco88I	CYCGRG	1 cut(s)	2132
EcoICRI	GAGCTC	1 cut(s)	838
EcoNI	CCTNNNNNAGG	1 cut(s)	37
EcoO109I	RGGNCCY	4 cut(s)	29, 425, 566, 1009
EcoRI	GAATTC	1 cut(s)	639
EcoRII	CCWGG	7 cut(s)	32, 318, 602, 727, 921, 1146, 1748
EcoRV	GATATC	1 cut(s)	1061
EcoT14I	CCWWGG	1 cut(s)	1324
EcoT38I	GRGCYC	2 cut(s)	840, 2131
ErhI	CCWWGG	1 cut(s)	1324
FaeI	CATG	9 cut(s)	329, 589, 626, 766, 959, 1328, 1713, 1941, 2077
FalI	AAGNNNNNCTT	2 cut(s)	1539, 1571
FaqI	GGGAC	7 cut(s)	342, 504, 700, 1235, 1291, 1785, 1936
FatI	CATG	9 cut(s)	325, 585, 622, 762, 955, 1324, 1709, 1937, 2073
FauNDI	CATATG	1 cut(s)	1741
FbaI	TGATCA	2 cut(s)	619, 2016
FblI	GTMKAC	1 cut(s)	64
Fnu4HI	GCNGC	5 cut(s)	194, 231, 554, 1227, 1841
FokI	GGATG	6 cut(s)	711, 1573, 1731, 1759, 1869, 2048
FriOI	GRGCYC	2 cut(s)	840, 2131
Fsp4HI	GCNGC	5 cut(s)	194, 231, 554, 1227, 1841
FspBI	CTAG	9 cut(s)	213, 422, 557, 1013, 1152, 1400, 1604, 1769, 1904
FspI	TGCGCA	1 cut(s)	1811
GlaI	GCGC	4 cut(s)	531, 1296, 1811, 1918
GluI	GCNGC	5 cut(s)	194, 231, 554, 1227, 1841
GsaI	CCCAGC	2 cut(s)	1726, 1968
GsuI	CTGGAG	3 cut(s)	302, 423, 2085
HaeII	RGCGCY	1 cut(s)	533
HaeIII	GGCC	7 cut(s)	323, 333, 340, 590, 801, 985, 1010
HapII	CCGG	4 cut(s)	93, 227, 354, 810
HgaI	GACGC	2 cut(s)	1232, 2157
HhaI	GCGC	4 cut(s)	532, 1297, 1812, 1919
Hin1II	CATG	9 cut(s)	329, 589, 626, 766, 959, 1328, 1713, 1941, 2077
Hin6I	GCGC	4 cut(s)	530, 1295, 1810, 1917
HinP1I	GCGC	4 cut(s)	530, 1295, 1810, 1917
HincII	GTYRAC	1 cut(s)	65
HindII	GTYRAC	1 cut(s)	65
HindIII	AAGCTT	1 cut(s)	1139
HinfI	GANTC	3 cut(s)	347, 613, 1900
HpaII	CCGG	4 cut(s)	93, 227, 354, 810
HphI	GGTGA	5 cut(s)	799, 944, 1136, 1985, 2051
Hpy166II	GTNNAC	7 cut(s)	65, 141, 218, 760, 1036, 1930, 2087
Hpy188I	TCNGA	7 cut(s)	159, 471, 1129, 1200, 1342, 1354, 2157
Hpy188III	TCNNGA	9 cut(s)	86, 131, 569, 617, 997, 1904, 2074, 2119, 2169
Hpy8I	GTNNAC	7 cut(s)	65, 141, 218, 760, 1036, 1930, 2087
Hpy99I	CGWCG	1 cut(s)	2151
HpyCH4III	ACNGT	2 cut(s)	889, 1655
HpyCH4IV	ACGT	3 cut(s)	57, 1032, 1572
HpyF10VI	GCNNNNNNNGC	8 cut(s)	278, 350, 413, 934, 1232, 1241, 1473, 1837
HpyF3I	CTNAG	8 cut(s)	38, 201, 299, 533, 690, 827, 846, 998
HpySE526I	ACGT	3 cut(s)	57, 1032, 1572
Hsp92II	CATG	9 cut(s)	329, 589, 626, 766, 959, 1328, 1713, 1941, 2077
HspAI	GCGC	4 cut(s)	530, 1295, 1810, 1917
KpnI	GGTACC	1 cut(s)	1749
Ksp22I	TGATCA	2 cut(s)	619, 2016
Kzo9I	GATC	9 cut(s)	82, 159, 175, 619, 821, 1117, 1173, 1981, 2016
LmnI	GCTCC	3 cut(s)	404, 1492, 1763
Lsp1109I	GCAGC	5 cut(s)	205, 242, 540, 1238, 1852
LweI	GCATC	8 cut(s)	79, 1426, 1476, 1595, 1709, 1781, 1951, 2148
MabI	ACCWGGT	1 cut(s)	1748
MaeI	CTAG	9 cut(s)	213, 422, 557, 1013, 1152, 1400, 1604, 1769, 1904
MaeII	ACGT	3 cut(s)	57, 1032, 1572
MaeIII	GTNAC	6 cut(s)	274, 397, 452, 1256, 1676, 2039
MalI	GATC	9 cut(s)	84, 161, 177, 621, 823, 1119, 1175, 1983, 2018
MboI	GATC	9 cut(s)	82, 159, 175, 619, 821, 1117, 1173, 1981, 2016
MflI	RGATCY	2 cut(s)	1117, 1981
MhlI	GDGCHC	4 cut(s)	840, 1032, 1768, 2131
MlsI	TGGCCA	2 cut(s)	333, 801
MluNI	TGGCCA	2 cut(s)	333, 801
MmeI	TCCRAC	4 cut(s)	616, 1365, 1497, 2009
Mox20I	TGGCCA	2 cut(s)	333, 801
MroXI	GAANNNNTTC	2 cut(s)	447, 969
MscI	TGGCCA	2 cut(s)	333, 801
MseI	TTAA	3 cut(s)	237, 864, 2163
MslI	CAYNNNNRTG	5 cut(s)	471, 590, 627, 767, 2156
Msp20I	TGGCCA	2 cut(s)	333, 801
MspI	CCGG	4 cut(s)	93, 227, 354, 810
MspR9I	CCNGG	7 cut(s)	34, 320, 604, 729, 923, 1148, 1750
Mva1269I	GAATGC	3 cut(s)	195, 1583, 2151
MvaI	CCWGG	7 cut(s)	34, 320, 604, 729, 923, 1148, 1750
MvnI	CGCG	1 cut(s)	1917
MwoI	GCNNNNNNNGC	8 cut(s)	278, 350, 413, 934, 1232, 1241, 1473, 1837
NcoI	CCATGG	1 cut(s)	1324
NdeI	CATATG	1 cut(s)	1741
NdeII	GATC	9 cut(s)	82, 159, 175, 619, 821, 1117, 1173, 1981, 2016
NlaIII	CATG	9 cut(s)	329, 589, 626, 766, 959, 1328, 1713, 1941, 2077
NlaIV	GGNNCC	5 cut(s)	31, 358, 567, 1494, 1747
NmuCI	GTSAC	3 cut(s)	274, 1256, 2039
NsbI	TGCGCA	1 cut(s)	1811
NspI	RCATGY	1 cut(s)	766
NspV	TTCGAA	1 cut(s)	1288
PagI	TCATGA	1 cut(s)	2073
PctI	GAATGC	3 cut(s)	195, 1583, 2151
PdmI	GAANNNNTTC	2 cut(s)	447, 969
PfeI	GAWTC	3 cut(s)	347, 613, 1900
PflMI	CCANNNNNTGG	1 cut(s)	609
PkrI	GCNGC	5 cut(s)	195, 232, 555, 1228, 1842
PmaCI	CACGTG	1 cut(s)	58
PmlI	CACGTG	1 cut(s)	58
Ppu21I	YACGTR	3 cut(s)	58, 1033, 1573
PpuMI	RGGWCCY	3 cut(s)	29, 425, 566
Psp124BI	GAGCTC	1 cut(s)	840
Psp5II	RGGWCCY	3 cut(s)	29, 425, 566
Psp6I	CCWGG	7 cut(s)	32, 318, 602, 727, 921, 1146, 1748
PspCI	CACGTG	1 cut(s)	58
PspFI	CCCAGC	2 cut(s)	1722, 1964
PspGI	CCWGG	7 cut(s)	32, 318, 602, 727, 921, 1146, 1748
PspN4I	GGNNCC	5 cut(s)	31, 358, 567, 1494, 1747
PspPI	GGNCC	9 cut(s)	29, 218, 322, 356, 425, 566, 984, 1009, 1321
PspPPI	RGGWCCY	3 cut(s)	29, 425, 566
PsrI	GAACNNNNNNTAC	2 cut(s)	111, 143
PsuI	RGATCY	2 cut(s)	1117, 1981
RseI	CAYNNNNRTG	5 cut(s)	471, 590, 627, 767, 2156
SacI	GAGCTC	1 cut(s)	840
SalI	GTCGAC	1 cut(s)	63
SaqAI	TTAA	3 cut(s)	237, 864, 2163
SatI	GCNGC	5 cut(s)	194, 231, 554, 1227, 1841
Sau3AI	GATC	9 cut(s)	82, 159, 175, 619, 821, 1117, 1173, 1981, 2016
Sau96I	GGNCC	9 cut(s)	29, 218, 322, 356, 425, 566, 984, 1009, 1321
ScrFI	CCNGG	7 cut(s)	34, 320, 604, 729, 923, 1148, 1750
SduI	GDGCHC	4 cut(s)	840, 1032, 1768, 2131
SexAI	ACCWGGT	1 cut(s)	1748
SfaNI	GCATC	8 cut(s)	79, 1426, 1476, 1595, 1709, 1781, 1951, 2148
SfuI	TTCGAA	1 cut(s)	1288
SinI	GGWCC	6 cut(s)	29, 218, 356, 425, 566, 1321
SmiMI	CAYNNNNRTG	5 cut(s)	471, 590, 627, 767, 2156
SnaBI	TACGTA	1 cut(s)	1573
SpeI	ACTAGT	1 cut(s)	1399
SsiI	CCGC	7 cut(s)	151, 632, 1476, 1584, 1590, 1915, 2143
SspMI	CTAG	9 cut(s)	213, 422, 557, 1013, 1152, 1400, 1604, 1769, 1904
SstI	GAGCTC	1 cut(s)	840
StyD4I	CCNGG	7 cut(s)	32, 318, 602, 727, 921, 1146, 1748
StyI	CCWWGG	1 cut(s)	1324
TaaI	ACNGT	2 cut(s)	889, 1655
TaiI	ACGT	3 cut(s)	60, 1035, 1575
TaqI	TCGA	8 cut(s)	64, 820, 1026, 1113, 1288, 1636, 1959, 2107
TatI	WGTACW	2 cut(s)	759, 2084
TfiI	GAWTC	3 cut(s)	347, 613, 1900
Tru1I	TTAA	3 cut(s)	237, 864, 2163
Tru9I	TTAA	3 cut(s)	237, 864, 2163
TscAI	CASTG	5 cut(s)	77, 279, 850, 892, 1477
TseFI	GTSAC	3 cut(s)	274, 1256, 2039
TseI	GCWGC	5 cut(s)	193, 230, 553, 1226, 1840
Tsp45I	GTSAC	3 cut(s)	274, 1256, 2039
TspDTI	ATGAA	8 cut(s)	358, 489, 713, 1056, 1698, 1926, 1985, 2062
TspGWI	ACGGA	1 cut(s)	1731
TspRI	CASTG	5 cut(s)	77, 279, 850, 892, 1477
Van91I	CCANNNNNTGG	1 cut(s)	609
VpaK11BI	GGWCC	6 cut(s)	29, 218, 356, 425, 566, 1321
XagI	CCTNNNNNAGG	1 cut(s)	37
XapI	RAATTY	5 cut(s)	77, 639, 1123, 1193, 1523
XbaI	TCTAGA	1 cut(s)	1903
XceI	RCATGY	1 cut(s)	766
XcmI	CCANNNNNNNNNTGG	2 cut(s)	326, 1167
XmiI	GTMKAC	1 cut(s)	64
XmnI	GAANNNNTTC	2 cut(s)	447, 969
XspI	CTAG	9 cut(s)	213, 422, 557, 1013, 1152, 1400, 1604, 1769, 1904

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.