Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr2

Orthogroup ID

OG0001409

Physical Location & Seq

Genomic Coordinates Reverse (-)

64746827 .. 64752755

Gene Structure 5929 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000010257

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2274 bp

ATGTGGAAGCTGAAGATTGCGGAGGGAGGACCCTGGCTGAGGACACTAAACAATCACGTGGGTCGACAACACTGGGAGTTTGATCCAGATGCCGGAACACCAGAAGAACGAGCCGAAGTGGAGAGAGTTCGTGAGGAGTTTACGAGAAACCGCTTTCGGATCAAACAAAGTGCTGATCTTTTGATGAGAATGCAGCTTACAAAGGAAAACCCTAGTGGACCAATTCCGGCTGCAATTAAAGTGAAGGGAATAAAGGATATAACAGAGAAGACAGTGACAGCAACAATAAGAAGATCTCTCAGCTTCTATTCCTCCCTCCAGGCCCATGATGGCCATTGGCCTGCTGAATCTGCCGGTCCCTTGTTCTTCATTCAACCTTTGGTTATAGCGTTGTATGTTACTGGAGCATTGTGTGCCGTTCTAGGACCTGAACACCAGAAGGAAATTATTCGTTACATCTACAATCATCAGAATGAAGATGGAGGTTGGGGACTACACATAGAAGGTCATAGCACGATGTTTGGGTCAGCGCTAAGCTACATTGCGTTGAGGCTGCTAGGAGAGGGTCCTGATGATGGGGAAGGCATGGCCGTGGCAAGAGCCAGGAAATGGATTCTTGATCATGGTGGTGCGGTTGGAATTCCATCTTGGGGAAAGTTTTGGCTTACGGTACTTGGCGTATATGAGTGGTCAGGGTGCAACCCTTTGCCCCCTGAGTTCTGGCTTCTACCAAATGTGTCCCCTATTCATCCAGGGAAGATGTTATGCTATTGTCGGTTGGTGTACATGCCTATGTCATACTTATACGGGAAGAGGTTTGTTGGCCGAATCACCGGATTGGTTCGATCACTAAGACGAGAGCTCTACACTGAACCATACTTTGACATTAACTGGCCTAAAGCCAGAAACGCTGTTGCAAAGGAAGATTTGTATTATCCACACCCCCTGGTGCAAGATATGCTGTGGGGATTTCTTCACCATGTTGCTGAACCAATTCTGGCTCGTTGGCCCTTTTCTATCCTGAGAAAGAAGGCCCTAGAAGTAGCAATCGAGCACGTACACTATGAAGACGAGAACAGCAGATATCTTTGCATTGGAAGTGTAGAAAAGGTGTTATGTTTGATTGCTTGTTGGGTCGAAGATCCAAATTCGGAAGCGTACAAGCTTCACCTGGCTAGAATACCAGATTACTTTTGGATCGCTGAAGATGGCTTGAAATTTCAGAGTTTCGGTAGTCAAATGTGGGACGCAGCCTTTGCAATTCAGGCAATACTCTCTTGTGACCTAAATGAAGAGTATGGGACAACACTTCGAAAAGCGCACGACTTTGTGAAGGCTTCACAGGTTCGAGAAAACCCTGGTGACAACTTCGCAGCTATGTACCGACACATATCTAAAGGCGCATGGACATTCTCAATACAGGACCATGGTTGGCAAGTCTCCGACTGCACTTCAGAAGGACTAAAGGTGGCACTCTTGCTCTCGCAAATGCCAGAGAAACTAGTTGGGAAAGGAATGGAAAGCGAATGTTTTTATGATGCTGTGAATGTCATTCTTTCTTTACAAAGCATCAGTGGCGGTTTCCCTGCTTGGGAGCCTCAAAGAGCGTTTCGTTGGTTGGAGAAATTCAACCCCACAGAGTTCTTTGAAGATACTCTTATTGAACGAGAGTACGTAGAATGCACCGCATCAGCAGTTCAAGGTCTAGCACTCTTTAGAAGATTGTATCCCAACCATCGAAGAAAGGAAATAGACAGTTGCATATCAAGGGCAATTCGTTACATTGAAGAAACACAAAACCCTGATGGTTCATGGTACGGATGCTGGGGTATTTGCTACACATATGGTACCTGGTTTGGTGTAGGAGCACTAGCATCCTGTGGGAAGAAGTACCAGAATAGTCCCACATTGCGCAAAGCCTGTGCATTTTTGCTATCAAAGCAGCTACCCAATGGTGGATGGGGAGAGAGCTACCTTTCCAGTCAAAATAAGGTTTATACAAATCTAGAAGGCAACCGCGCTAATCTGGTACACACTGCATGGGCTTTATTGTCCCTCATCGATGCCGGGCAGGGTGATGTAGATCCAACCCCCATTCATCGTGGAATAATGGTTTTGATCAATTCACAAATGGAGGATGGTGACTTCCCTCAACAGGAAATTACTGGAGTATTCATGAGAAATTGTACACTAAACTACTCCTCATATCGAAATATCTTCCCGAAATGGGCTCTCGGGGCATACCGCCGACGCATTCTGATGCTTACATCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0006629 GO:0006720 GO:0006721 GO:0006722 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009987 GO:0016104 GO:0016114 GO:0016853 GO:0016866 GO:0019742 GO:0019745 GO:0031559 GO:0042299 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0071704 GO:1901360 GO:1901362 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00909 ko01110 map00909 map01110

Pfam Domains

Protein Families

SQHop_cyclase_C SQHop_cyclase_N

Protein Analysis

758

Amino Acids

86.24

Weight (kDa)

6.75

Isoelectric Point (pI)

47.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SQHop_cyclase_N	PF13249	96 - 358	2e-38	Squalene-hopene cyclase N-terminal domain
SQHop_cyclase_C	PF13243	412 - 750	1.5e-52	Squalene-hopene cyclase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001409)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g10860 FvH4_7g10860 FvH4_7g10860
malus_domestica	MD17G1158900.v1.1 MD17G1160000.v1.1 MD17G1246000.v1.1 MD17G1246100.v1.1 MD17G1246500.v1.1 MD17G1246700.v1.1
prunus_persica	Prupe.1G002600_v2.0.a1 Prupe.1G002600_v2.0.a1 Prupe.7G043400_v2.0.a1
pyrus_communis	pycom16g19870 pycom16g24410
rosa_chinensis	RchiOBHm_Chr1g0332711 RchiOBHm_Chr2g0148781 RchiOBHm_Chr4g0385071 RchiOBHm_Chr4g0385101
rosa_laevigata	RLG00000010257
rosa_multiflora	Rmu_co8015556.1_g000001 Rmu_co8308753.1_g000001 Rmu_co8434809.1_g000001 Rmu_sc0000824.1_g000004 Rmu_sc0001548.1_g000031 Rmu_sc0001548.1_g000053 Rmu_sc0002070.1_g000040 Rmu_sc0002116.1_g000014 Rmu_sc0003929.1_g000004 Rmu_sc0008546.1_g000012 Rmu_sc0008678.1_g000005 Rmu_ssc0000158.1_g000036
rosa_roxburghii	Rroxscaffold_5G00333010 Rroxscaffold_5G00342310
rosa_rugosa	Rorug03G0113700 Rorug03G0225800 Rorug03G0298900
rosa_samantha	Rh1CG121300 Rh1DG132500 Rh2AG466900 Rh2CG453300 Rh2CG453700 Rh4AG002600 Rh4AG078200 Rh4AG078500 Rh4BG002100 Rh4BG073600 Rh4BG073800 Rh4BG075600 Rh4BG076300 Rh4DG002100 Rh4DG071700 Rh7BG219000
rosa_wichuraiana	Rw4G000670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1913
Acc65I	GGTACC	1 cut(s)	1847
AccB1I	GGYRCC	1 cut(s)	1847
AccB7I	CCANNNNNTGG	1 cut(s)	609
AccI	GTMKAC	1 cut(s)	64
AccII	CGCG	1 cut(s)	2019
AciI	CCGC	7 cut(s)	20, 151, 632, 1578, 1686, 2017, 2245
AclWI	GGATC	5 cut(s)	77, 167, 1136, 1205, 2078
AcoI	YGGCCR	3 cut(s)	331, 588, 823
AcsI	RAATTY	4 cut(s)	639, 1147, 1217, 1625
AcuI	CTGAAG	3 cut(s)	32, 1224, 1437
AcvI	CACGTG	1 cut(s)	58
AfeI	AGCGCT	1 cut(s)	531
AfiI	CCNNNNNNNGG	9 cut(s)	39, 92, 575, 609, 650, 1591, 1955, 2155, 2227
AgsI	TTSAA	7 cut(s)	374, 1216, 1630, 1649, 1664, 1700, 1787
AhlI	ACTAGT	1 cut(s)	1501
AjnI	CCWGG	8 cut(s)	32, 318, 602, 751, 945, 1170, 1357, 1850
AjuI	GAANNNNNNNTTGG	2 cut(s)	631, 663
AluBI	AGCT	9 cut(s)	10, 196, 303, 537, 862, 1165, 1376, 1945, 1971
AluI	AGCT	9 cut(s)	10, 196, 303, 537, 862, 1165, 1376, 1945, 1971
Alw21I	GWGCWC	3 cut(s)	864, 1056, 1870
Alw26I	GTCTC	1 cut(s)	1444
AlwI	GGATC	5 cut(s)	77, 167, 1136, 1205, 2078
Ama87I	CYCGRG	1 cut(s)	2234
Aor51HI	AGCGCT	1 cut(s)	531
AoxI	GGCC	8 cut(s)	321, 331, 338, 588, 823, 893, 1007, 1032
ApeKI	GCWGC	6 cut(s)	193, 230, 553, 1250, 1373, 1942
ApoI	RAATTY	4 cut(s)	639, 1147, 1217, 1625
ArsI	GACNNNNNNTTYG	2 cut(s)	1238, 1270
Asp700I	GAANNNNTTC	2 cut(s)	447, 993
Asp718I	GGTACC	1 cut(s)	1847
AspLEI	GCGC	5 cut(s)	532, 1321, 1403, 1914, 2021
AspS9I	GGNCC	9 cut(s)	29, 218, 322, 356, 425, 566, 1008, 1033, 1423
AsuC2I	CCSGG	1 cut(s)	2068
AsuHPI	GGTGA	6 cut(s)	823, 968, 1160, 1373, 2087, 2153
AsuII	TTCGAA	1 cut(s)	1312
AvaI	CYCGRG	1 cut(s)	2234
AvaII	GGWCC	6 cut(s)	29, 218, 356, 425, 566, 1423
BalI	TGGCCA	1 cut(s)	333
BanI	GGYRCC	1 cut(s)	1847
BanII	GRGCYC	2 cut(s)	864, 2233
BbrPI	CACGTG	1 cut(s)	58
BbsI	GAAGAC	2 cut(s)	275, 1074
Bbv12I	GWGCWC	3 cut(s)	864, 1056, 1870
BbvCI	CCTCAGC	1 cut(s)	38
BbvI	GCAGC	6 cut(s)	205, 217, 540, 1262, 1385, 1954
BccI	CCATC	9 cut(s)	323, 473, 569, 652, 1202, 1743, 1799, 1953, 2132
BceAI	ACGGC	2 cut(s)	401, 575
BciT130I	CCWGG	8 cut(s)	34, 320, 604, 753, 947, 1172, 1359, 1852
BciVI	GTATCC	1 cut(s)	1737
BclI	TGATCA	2 cut(s)	619, 2118
BcnI	CCSGG	1 cut(s)	2068
BcoDI	GTCTC	1 cut(s)	1444
BcuI	ACTAGT	1 cut(s)	1501
BfaI	CTAG	9 cut(s)	213, 422, 557, 1037, 1176, 1502, 1706, 1871, 2006
BfoI	RGCGCY	1 cut(s)	533
BfuI	GTATCC	1 cut(s)	1737
BglII	AGATCT	1 cut(s)	293
BisI	GCNGC	6 cut(s)	194, 231, 554, 1251, 1374, 1943
BlpI	GCTNAGC	1 cut(s)	533
BlsI	GCNGC	6 cut(s)	195, 232, 555, 1252, 1375, 1944
Bme1390I	CCNGG	9 cut(s)	34, 320, 604, 753, 947, 1172, 1359, 1852, 2068
Bme18I	GGWCC	6 cut(s)	29, 218, 356, 425, 566, 1423
BmeT110I	CYCGRG	1 cut(s)	2234
BmgT120I	GGNCC	9 cut(s)	29, 218, 322, 356, 425, 566, 1008, 1033, 1423
BmiI	GGNNCC	5 cut(s)	31, 358, 567, 1596, 1849
BmrFI	CCNGG	9 cut(s)	34, 320, 604, 753, 947, 1172, 1359, 1852, 2068
BmrI	ACTGGG	1 cut(s)	82
BmsI	GCATC	8 cut(s)	79, 1528, 1578, 1697, 1811, 1883, 2053, 2250
BmuI	ACTGGG	1 cut(s)	82
BpiI	GAAGAC	2 cut(s)	275, 1074
BpmI	CTGGAG	3 cut(s)	302, 423, 2187
Bpu10I	CCTNAGC	1 cut(s)	38
Bpu1102I	GCTNAGC	1 cut(s)	533
Bpu14I	TTCGAA	1 cut(s)	1312
BpuMI	CCSGG	1 cut(s)	2068
Bsa29I	ATCGAT	1 cut(s)	2061
BsaAI	YACGTR	3 cut(s)	58, 1057, 1675
BsaBI	GATNNNNATC	1 cut(s)	2082
BsaJI	CCNNGG	6 cut(s)	32, 591, 752, 945, 1357, 1426
BsaWI	WCCGGW	1 cut(s)	833
Bsc4I	CCNNNNNNNGG	9 cut(s)	39, 92, 575, 609, 650, 1591, 1955, 2155, 2227
Bse118I	RCCGGY	1 cut(s)	353
Bse1I	ACTGG	5 cut(s)	77, 406, 896, 1980, 2170
Bse3DI	GCAATG	2 cut(s)	540, 1907
Bse8I	GATNNNNATC	1 cut(s)	2082
BseBI	CCWGG	8 cut(s)	34, 320, 604, 753, 947, 1172, 1359, 1852
BseCI	ATCGAT	1 cut(s)	2061
BseDI	CCNNGG	6 cut(s)	32, 591, 752, 945, 1357, 1426
BseGI	GGATG	5 cut(s)	748, 1826, 1874, 1964, 2143
BseJI	GATNNNNATC	1 cut(s)	2082
BseLI	CCNNNNNNNGG	9 cut(s)	39, 92, 575, 609, 650, 1591, 1955, 2155, 2227
BseMI	GCAATG	2 cut(s)	540, 1907
BseMII	CTCAG	4 cut(s)	29, 313, 705, 1013
BseNI	ACTGG	5 cut(s)	77, 406, 896, 1980, 2170
BseRI	GAGGAG	2 cut(s)	149, 2191
BseXI	GCAGC	6 cut(s)	205, 217, 540, 1262, 1385, 1954
BseYI	CCCAGC	1 cut(s)	1824
BsgI	GTGCAG	1 cut(s)	1432
Bsh1236I	CGCG	1 cut(s)	2019
BshFI	GGCC	8 cut(s)	323, 333, 340, 590, 825, 895, 1009, 1034
BshNI	GGYRCC	1 cut(s)	1847
BshVI	ATCGAT	1 cut(s)	2061
BsiHKAI	GWGCWC	3 cut(s)	864, 1056, 1870
BsiHKCI	CYCGRG	1 cut(s)	2234
BsiSI	CCGG	5 cut(s)	93, 227, 354, 834, 2067
BslFI	GGGAC	7 cut(s)	342, 504, 724, 1259, 1315, 1887, 2038
BslI	CCNNNNNNNGG	9 cut(s)	39, 92, 575, 609, 650, 1591, 1955, 2155, 2227
BsmAI	GTCTC	1 cut(s)	1444
BsmFI	GGGAC	7 cut(s)	342, 504, 724, 1259, 1315, 1887, 2038
BsmI	GAATGC	3 cut(s)	195, 1685, 2253
BsnI	GGCC	8 cut(s)	323, 333, 340, 590, 825, 895, 1009, 1034
BsoBI	CYCGRG	1 cut(s)	2234
Bsp119I	TTCGAA	1 cut(s)	1312
Bsp1286I	GDGCHC	4 cut(s)	864, 1056, 1870, 2233
Bsp1407I	TGTACA	2 cut(s)	783, 2186
Bsp1720I	GCTNAGC	1 cut(s)	533
Bsp19I	CCATGG	1 cut(s)	1426
BspACI	CCGC	7 cut(s)	20, 151, 632, 1578, 1686, 2017, 2245
BspANI	GGCC	8 cut(s)	323, 333, 340, 590, 825, 895, 1009, 1034
BspCNI	CTCAG	4 cut(s)	30, 312, 706, 1014
BspDI	ATCGAT	1 cut(s)	2061
BspFNI	CGCG	1 cut(s)	2019
BspHI	TCATGA	1 cut(s)	2175
BspLI	GGNNCC	5 cut(s)	31, 358, 567, 1596, 1849
BspPI	GGATC	5 cut(s)	77, 167, 1136, 1205, 2078
BspT104I	TTCGAA	1 cut(s)	1312
BspT107I	GGYRCC	1 cut(s)	1847
BsrDI	GCAATG	2 cut(s)	540, 1907
BsrFI	RCCGGY	1 cut(s)	353
BsrGI	TGTACA	2 cut(s)	783, 2186
BsrI	ACTGG	5 cut(s)	77, 406, 896, 1980, 2170
BssAI	RCCGGY	1 cut(s)	353
BssECI	CCNNGG	6 cut(s)	32, 591, 752, 945, 1357, 1426
BssT1I	CCWWGG	1 cut(s)	1426
Bst2UI	CCWGG	8 cut(s)	34, 320, 604, 753, 947, 1172, 1359, 1852
Bst4CI	ACNGT	3 cut(s)	274, 670, 1757
Bst6I	CTCTTC	2 cut(s)	806, 1287
BstAPI	GCANNNNNTGC	3 cut(s)	413, 958, 1256
BstAUI	TGTACA	2 cut(s)	783, 2186
BstBAI	YACGTR	3 cut(s)	58, 1057, 1675
BstBI	TTCGAA	1 cut(s)	1312
BstC8I	GCNNGC	1 cut(s)	342
BstDEI	CTNAG	6 cut(s)	38, 299, 533, 714, 851, 1022
BstDSI	CCRYGG	2 cut(s)	591, 1426
BstENI	CCTNNNNNAGG	1 cut(s)	37
BstF5I	GGATG	5 cut(s)	748, 1826, 1874, 1964, 2143
BstFNI	CGCG	1 cut(s)	2019
BstH2I	RGCGCY	1 cut(s)	533
BstHHI	GCGC	5 cut(s)	532, 1321, 1403, 1914, 2021
BstMAI	GTCTC	1 cut(s)	1444
BstMWI	GCNNNNNNNGC	9 cut(s)	350, 413, 908, 958, 1256, 1265, 1575, 1939, 2237
BstNI	CCWGG	8 cut(s)	34, 320, 604, 753, 947, 1172, 1359, 1852
BstNSI	RCATGY	1 cut(s)	790
BstSCI	CCNGG	9 cut(s)	32, 318, 602, 751, 945, 1170, 1357, 1850, 2066
BstSNI	TACGTA	1 cut(s)	1675
BstUI	CGCG	1 cut(s)	2019
BstV1I	GCAGC	6 cut(s)	205, 217, 540, 1262, 1385, 1954
BstV2I	GAAGAC	2 cut(s)	275, 1074
BstX2I	RGATCY	3 cut(s)	293, 1141, 2083
BstYI	RGATCY	3 cut(s)	293, 1141, 2083
Bsu15I	ATCGAT	1 cut(s)	2061
BsuI	GTATCC	1 cut(s)	1737
BsuRI	GGCC	8 cut(s)	323, 333, 340, 590, 825, 895, 1009, 1034
BsuTUI	ATCGAT	1 cut(s)	2061
BtgI	CCRYGG	2 cut(s)	591, 1426
BtsCI	GGATG	5 cut(s)	748, 1826, 1874, 1964, 2143
BtsI	GCAGTG	1 cut(s)	2034
BtsIMutI	CAGTG	5 cut(s)	70, 279, 867, 1579, 2034
Cac8I	GCNNGC	1 cut(s)	342
CciI	TCATGA	1 cut(s)	2175
CfoI	GCGC	5 cut(s)	532, 1321, 1403, 1914, 2021
Cfr10I	RCCGGY	1 cut(s)	353
Cfr13I	GGNCC	9 cut(s)	29, 218, 322, 356, 425, 566, 1008, 1033, 1423
ClaI	ATCGAT	1 cut(s)	2061
CseI	GACGC	2 cut(s)	1256, 2259
CsiI	ACCWGGT	1 cut(s)	1850
DdeI	CTNAG	6 cut(s)	38, 299, 533, 714, 851, 1022
EaeI	YGGCCR	3 cut(s)	331, 588, 823
Eam1104I	CTCTTC	2 cut(s)	806, 1287
EarI	CTCTTC	2 cut(s)	806, 1287
Ecl136II	GAGCTC	1 cut(s)	862
Eco105I	TACGTA	1 cut(s)	1675
Eco130I	CCWWGG	1 cut(s)	1426
Eco24I	GRGCYC	2 cut(s)	864, 2233
Eco32I	GATATC	1 cut(s)	1085
Eco47I	GGWCC	6 cut(s)	29, 218, 356, 425, 566, 1423
Eco47III	AGCGCT	1 cut(s)	531
Eco53kI	GAGCTC	1 cut(s)	862
Eco57I	CTGAAG	3 cut(s)	32, 1224, 1437
Eco72I	CACGTG	1 cut(s)	58
Eco88I	CYCGRG	1 cut(s)	2234
EcoICRI	GAGCTC	1 cut(s)	862
EcoNI	CCTNNNNNAGG	1 cut(s)	37
EcoO109I	RGGNCCY	4 cut(s)	29, 425, 566, 1033
EcoRI	GAATTC	1 cut(s)	639
EcoRII	CCWGG	8 cut(s)	32, 318, 602, 751, 945, 1170, 1357, 1850
EcoRV	GATATC	1 cut(s)	1085
EcoT14I	CCWWGG	1 cut(s)	1426
EcoT38I	GRGCYC	2 cut(s)	864, 2233
ErhI	CCWWGG	1 cut(s)	1426
FalI	AAGNNNNNCTT	2 cut(s)	1641, 1673
FaqI	GGGAC	7 cut(s)	342, 504, 724, 1259, 1315, 1887, 2038
FauNDI	CATATG	1 cut(s)	1843
FbaI	TGATCA	2 cut(s)	619, 2118
FblI	GTMKAC	1 cut(s)	64
Fnu4HI	GCNGC	6 cut(s)	194, 231, 554, 1251, 1374, 1943
FokI	GGATG	5 cut(s)	735, 1833, 1861, 1971, 2150
FriOI	GRGCYC	2 cut(s)	864, 2233
Fsp4HI	GCNGC	6 cut(s)	194, 231, 554, 1251, 1374, 1943
FspBI	CTAG	9 cut(s)	213, 422, 557, 1037, 1176, 1502, 1706, 1871, 2006
FspI	TGCGCA	1 cut(s)	1913
GlaI	GCGC	5 cut(s)	531, 1320, 1402, 1913, 2020
GluI	GCNGC	6 cut(s)	194, 231, 554, 1251, 1374, 1943
GsaI	CCCAGC	1 cut(s)	1828
GsuI	CTGGAG	3 cut(s)	302, 423, 2187
HaeII	RGCGCY	1 cut(s)	533
HaeIII	GGCC	8 cut(s)	323, 333, 340, 590, 825, 895, 1009, 1034
HapII	CCGG	5 cut(s)	93, 227, 354, 834, 2067
HgaI	GACGC	2 cut(s)	1256, 2259
HhaI	GCGC	5 cut(s)	532, 1321, 1403, 1914, 2021
Hin6I	GCGC	5 cut(s)	530, 1319, 1401, 1912, 2019
HinP1I	GCGC	5 cut(s)	530, 1319, 1401, 1912, 2019
HincII	GTYRAC	1 cut(s)	65
HindII	GTYRAC	1 cut(s)	65
HindIII	AAGCTT	1 cut(s)	1163
HinfI	GANTC	3 cut(s)	347, 613, 828
HpaII	CCGG	5 cut(s)	93, 227, 354, 834, 2067
HphI	GGTGA	6 cut(s)	823, 968, 1160, 1373, 2087, 2153
Hpy166II	GTNNAC	7 cut(s)	65, 141, 218, 784, 1060, 2032, 2189
Hpy188I	TCNGA	7 cut(s)	159, 471, 1153, 1224, 1444, 1456, 2259
Hpy8I	GTNNAC	7 cut(s)	65, 141, 218, 784, 1060, 2032, 2189
Hpy99I	CGWCG	1 cut(s)	2253
HpyAV	CCTTC	8 cut(s)	238, 433, 497, 575, 1024, 1327, 1451, 2003
HpyCH4III	ACNGT	3 cut(s)	274, 670, 1757
HpyCH4IV	ACGT	3 cut(s)	57, 1056, 1674
HpyF10VI	GCNNNNNNNGC	9 cut(s)	350, 413, 908, 958, 1256, 1265, 1575, 1939, 2237
HpyF3I	CTNAG	6 cut(s)	38, 299, 533, 714, 851, 1022
HpySE526I	ACGT	3 cut(s)	57, 1056, 1674
HspAI	GCGC	5 cut(s)	530, 1319, 1401, 1912, 2019
KpnI	GGTACC	1 cut(s)	1851
Ksp22I	TGATCA	2 cut(s)	619, 2118
LmnI	GCTCC	3 cut(s)	404, 1594, 1865
Lsp1109I	GCAGC	6 cut(s)	205, 217, 540, 1262, 1385, 1954
LweI	GCATC	8 cut(s)	79, 1528, 1578, 1697, 1811, 1883, 2053, 2250
MabI	ACCWGGT	1 cut(s)	1850
MaeI	CTAG	9 cut(s)	213, 422, 557, 1037, 1176, 1502, 1706, 1871, 2006
MaeII	ACGT	3 cut(s)	57, 1056, 1674
MaeIII	GTNAC	7 cut(s)	274, 397, 452, 1280, 1361, 1778, 2141
MflI	RGATCY	3 cut(s)	293, 1141, 2083
MhlI	GDGCHC	4 cut(s)	864, 1056, 1870, 2233
MlsI	TGGCCA	1 cut(s)	333
MluNI	TGGCCA	1 cut(s)	333
MmeI	TCCRAC	4 cut(s)	616, 1467, 1599, 2111
Mox20I	TGGCCA	1 cut(s)	333
MroXI	GAANNNNTTC	2 cut(s)	447, 993
MscI	TGGCCA	1 cut(s)	333
MseI	TTAA	2 cut(s)	237, 888
MslI	CAYNNNNRTG	5 cut(s)	471, 590, 627, 791, 2258
Msp20I	TGGCCA	1 cut(s)	333
MspI	CCGG	5 cut(s)	93, 227, 354, 834, 2067
MspR9I	CCNGG	9 cut(s)	34, 320, 604, 753, 947, 1172, 1359, 1852, 2068
Mva1269I	GAATGC	3 cut(s)	195, 1685, 2253
MvaI	CCWGG	8 cut(s)	34, 320, 604, 753, 947, 1172, 1359, 1852
MvnI	CGCG	1 cut(s)	2019
MwoI	GCNNNNNNNGC	9 cut(s)	350, 413, 908, 958, 1256, 1265, 1575, 1939, 2237
NciI	CCSGG	1 cut(s)	2068
NcoI	CCATGG	1 cut(s)	1426
NdeI	CATATG	1 cut(s)	1843
NlaIV	GGNNCC	5 cut(s)	31, 358, 567, 1596, 1849
NmuCI	GTSAC	4 cut(s)	274, 1280, 1361, 2141
NsbI	TGCGCA	1 cut(s)	1913
NspI	RCATGY	1 cut(s)	790
NspV	TTCGAA	1 cut(s)	1312
PagI	TCATGA	1 cut(s)	2175
PctI	GAATGC	3 cut(s)	195, 1685, 2253
PdmI	GAANNNNTTC	2 cut(s)	447, 993
PfeI	GAWTC	3 cut(s)	347, 613, 828
PflMI	CCANNNNNTGG	1 cut(s)	609
PkrI	GCNGC	6 cut(s)	195, 232, 555, 1252, 1375, 1944
PmaCI	CACGTG	1 cut(s)	58
PmlI	CACGTG	1 cut(s)	58
Ppu21I	YACGTR	3 cut(s)	58, 1057, 1675
PpuMI	RGGWCCY	3 cut(s)	29, 425, 566
Psp124BI	GAGCTC	1 cut(s)	864
Psp5II	RGGWCCY	3 cut(s)	29, 425, 566
Psp6I	CCWGG	8 cut(s)	32, 318, 602, 751, 945, 1170, 1357, 1850
PspCI	CACGTG	1 cut(s)	58
PspFI	CCCAGC	1 cut(s)	1824
PspGI	CCWGG	8 cut(s)	32, 318, 602, 751, 945, 1170, 1357, 1850
PspN4I	GGNNCC	5 cut(s)	31, 358, 567, 1596, 1849
PspPI	GGNCC	9 cut(s)	29, 218, 322, 356, 425, 566, 1008, 1033, 1423
PspPPI	RGGWCCY	3 cut(s)	29, 425, 566
PsuI	RGATCY	3 cut(s)	293, 1141, 2083
RseI	CAYNNNNRTG	5 cut(s)	471, 590, 627, 791, 2258
SacI	GAGCTC	1 cut(s)	864
SalI	GTCGAC	1 cut(s)	63
SaqAI	TTAA	2 cut(s)	237, 888
SatI	GCNGC	6 cut(s)	194, 231, 554, 1251, 1374, 1943
Sau96I	GGNCC	9 cut(s)	29, 218, 322, 356, 425, 566, 1008, 1033, 1423
ScrFI	CCNGG	9 cut(s)	34, 320, 604, 753, 947, 1172, 1359, 1852, 2068
SduI	GDGCHC	4 cut(s)	864, 1056, 1870, 2233
SexAI	ACCWGGT	1 cut(s)	1850
SfaNI	GCATC	8 cut(s)	79, 1528, 1578, 1697, 1811, 1883, 2053, 2250
SfuI	TTCGAA	1 cut(s)	1312
SinI	GGWCC	6 cut(s)	29, 218, 356, 425, 566, 1423
SmiMI	CAYNNNNRTG	5 cut(s)	471, 590, 627, 791, 2258
SnaBI	TACGTA	1 cut(s)	1675
SpeI	ACTAGT	1 cut(s)	1501
SsiI	CCGC	7 cut(s)	20, 151, 632, 1578, 1686, 2017, 2245
SspMI	CTAG	9 cut(s)	213, 422, 557, 1037, 1176, 1502, 1706, 1871, 2006
SstI	GAGCTC	1 cut(s)	864
StyD4I	CCNGG	9 cut(s)	32, 318, 602, 751, 945, 1170, 1357, 1850, 2066
StyI	CCWWGG	1 cut(s)	1426
TaaI	ACNGT	3 cut(s)	274, 670, 1757
TaiI	ACGT	3 cut(s)	60, 1059, 1677
TaqI	TCGA	9 cut(s)	64, 844, 1050, 1137, 1312, 1348, 1738, 2061, 2209
TatI	WGTACW	2 cut(s)	783, 2186
TfiI	GAWTC	3 cut(s)	347, 613, 828
Tru1I	TTAA	2 cut(s)	237, 888
Tru9I	TTAA	2 cut(s)	237, 888
TscAI	CASTG	5 cut(s)	77, 279, 874, 1579, 2041
TseFI	GTSAC	4 cut(s)	274, 1280, 1361, 2141
TseI	GCWGC	6 cut(s)	193, 230, 553, 1250, 1373, 1942
Tsp45I	GTSAC	4 cut(s)	274, 1280, 1361, 2141
TspDTI	ATGAA	8 cut(s)	358, 489, 737, 1080, 1305, 1800, 2087, 2164
TspGWI	ACGGA	1 cut(s)	1833
TspRI	CASTG	5 cut(s)	77, 279, 874, 1579, 2041
Van91I	CCANNNNNTGG	1 cut(s)	609
VpaK11BI	GGWCC	6 cut(s)	29, 218, 356, 425, 566, 1423
XagI	CCTNNNNNAGG	1 cut(s)	37
XapI	RAATTY	4 cut(s)	639, 1147, 1217, 1625
XbaI	TCTAGA	1 cut(s)	2005
XceI	RCATGY	1 cut(s)	790
XcmI	CCANNNNNNNNNTGG	2 cut(s)	326, 1191
XmiI	GTMKAC	1 cut(s)	64
XmnI	GAANNNNTTC	2 cut(s)	447, 993
XspI	CTAG	9 cut(s)	213, 422, 557, 1037, 1176, 1502, 1706, 1871, 2006

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.