Prupe.1G019200_v2.0.a1

transcription, DNA-templated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0002665
Physical Location & Seq
Forward (+)
1346049 .. 1347329
1281 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G019200.1

Sequence Viewer

Length: 522 bp
ATGACACGTCCAACCTCTTCTCCCTCGTCGTCAAGACTGGGACCCGTGACGCCCTTCTATGACTCGGCGAACTCGGCCGCCAACTCAGTCCCCGTCGTCAAATCTGTCATCAACTTCCTCGACGACCGTTACTTCTCCGACGTGGACGGTCAAGACGCAGAGACTGAGGTGAGCAGGGAAGAGGCCGAGGCTGCTTCATGGCTGCTCCCAAATATGAAGGCAATGGAGAATCCGGATCTGAACTCGGGCGATTACTTTCTCCCGGAAATTGTTCCGTATTTGGATTTGGACTACGGGCATGTGGATCCGTTTTATTTATTTTGGTATTTGTTCGGTATTTTGTTATTGATTTGTTTTATGGTTAGGCATATGCTGGAGAAGTTGGGGTGGGTTGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGGTGGTTTTGGTGGTAGGGGTAGGTCAAATAGAGGAGGGTTTGATGGTCGAGGAGGATCAGACGGACCACGTCATCAAAAAAAAGGGTTCTATTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

174

Amino Acids

19.65

Weight (kDa)

5.11

Isoelectric Point (pI)

44.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 232
AciI CCGC 1 cut(s) 78
AclWI GGATC 4 cut(s) 243, 299, 312, 487
AcoI YGGCCR 1 cut(s) 75
AcyI GRCGYC 1 cut(s) 50
AflIII ACRYGT 1 cut(s) 5
AjiI CACGTC 3 cut(s) 8, 142, 494
Alw26I GTCTC 1 cut(s) 155
AlwI GGATC 4 cut(s) 243, 299, 312, 487
AlwNI CAGNNNCTG 1 cut(s) 164
Ama87I CYCGRG 1 cut(s) 244
Aor13HI TCCGGA 1 cut(s) 232
AoxI GGCC 2 cut(s) 75, 183
ApeKI GCWGC 2 cut(s) 191, 202
Asp700I GAANNNNTTC 1 cut(s) 270
AspS9I GGNCC 2 cut(s) 41, 488
AsuC2I CCSGG 1 cut(s) 263
AsuHPI GGTGA 1 cut(s) 181
AvaI CYCGRG 1 cut(s) 244
AvaII GGWCC 2 cut(s) 41, 488
BamHI GGATCC 1 cut(s) 304
BbvI GCAGC 2 cut(s) 178, 189
BccI CCATC 1 cut(s) 461
BcnI CCSGG 1 cut(s) 263
BcoDI GTCTC 1 cut(s) 155
BisI GCNGC 3 cut(s) 78, 192, 203
BlsI GCNGC 3 cut(s) 79, 193, 204
Bme1390I CCNGG 1 cut(s) 263
Bme18I GGWCC 2 cut(s) 41, 488
BmeT110I CYCGRG 1 cut(s) 244
BmgBI CACGTC 3 cut(s) 8, 142, 494
BmgT120I GGNCC 2 cut(s) 41, 488
BmiI GGNNCC 3 cut(s) 42, 43, 306
BmrFI CCNGG 1 cut(s) 263
BmrI ACTGGG 1 cut(s) 47
BmuI ACTGGG 1 cut(s) 47
BpmI CTGGAG 1 cut(s) 395
BpuMI CCSGG 1 cut(s) 263
BsaHI GRCGYC 1 cut(s) 50
BsaJI CCNNGG 1 cut(s) 186
BsaWI WCCGGW 1 cut(s) 232
BsaXI ACNNNNNCTCC 1 cut(s) 34
Bse1I ACTGG 1 cut(s) 42
Bse3DI GCAATG 1 cut(s) 228
BseAI TCCGGA 1 cut(s) 232
BseDI CCNNGG 1 cut(s) 186
BseMI GCAATG 1 cut(s) 228
BseMII CTCAG 2 cut(s) 99, 156
BseNI ACTGG 1 cut(s) 42
BseRI GAGGAG 2 cut(s) 471, 489
BseX3I CGGCCG 1 cut(s) 75
BseXI GCAGC 2 cut(s) 178, 189
Bsh1285I CGRYCG 2 cut(s) 78, 127
BshFI GGCC 2 cut(s) 77, 185
BsiEI CGRYCG 2 cut(s) 78, 127
BsiHKCI CYCGRG 1 cut(s) 244
BsiSI CCGG 2 cut(s) 233, 263
BslFI GGGAC 2 cut(s) 54, 74
BsmAI GTCTC 1 cut(s) 155
BsmFI GGGAC 2 cut(s) 54, 74
BsnI GGCC 2 cut(s) 77, 185
BsoBI CYCGRG 1 cut(s) 244
Bsp13I TCCGGA 1 cut(s) 232
Bsp143I GATC 3 cut(s) 235, 304, 479
BspACI CCGC 1 cut(s) 78
BspANI GGCC 2 cut(s) 77, 185
BspCNI CTCAG 2 cut(s) 98, 157
BspEI TCCGGA 1 cut(s) 232
BspLI GGNNCC 3 cut(s) 42, 43, 306
BspPI GGATC 4 cut(s) 243, 299, 312, 487
BsrDI GCAATG 1 cut(s) 228
BsrI ACTGG 1 cut(s) 42
BssECI CCNNGG 1 cut(s) 186
BssMI GATC 3 cut(s) 235, 304, 479
BssNI GRCGYC 1 cut(s) 50
Bst4CI ACNGT 2 cut(s) 128, 149
Bst6I CTCTTC 2 cut(s) 22, 174
BstACI GRCGYC 1 cut(s) 50
BstDEI CTNAG 2 cut(s) 85, 165
BstKTI GATC 3 cut(s) 238, 307, 482
BstMAI GTCTC 1 cut(s) 155
BstMBI GATC 3 cut(s) 235, 304, 479
BstMCI CGRYCG 2 cut(s) 78, 127
BstMWI GCNNNNNNNGC 2 cut(s) 74, 191
BstNSI RCATGY 1 cut(s) 302
BstSCI CCNGG 1 cut(s) 261
BstV1I GCAGC 2 cut(s) 178, 189
BstX2I RGATCY 2 cut(s) 235, 304
BstYI RGATCY 2 cut(s) 235, 304
BstZI CGGCCG 1 cut(s) 75
BsuRI GGCC 2 cut(s) 77, 185
BtrI CACGTC 3 cut(s) 8, 142, 494
CaiI CAGNNNCTG 1 cut(s) 164
Cfr13I GGNCC 2 cut(s) 41, 488
CseI GACGC 2 cut(s) 58, 164
CviAII CATG 2 cut(s) 198, 299
CviJI RGCY 4 cut(s) 77, 185, 191, 202
CviKI_1 RGCY 4 cut(s) 77, 185, 191, 202
DdeI CTNAG 2 cut(s) 85, 165
DpnI GATC 3 cut(s) 237, 306, 481
DpnII GATC 3 cut(s) 235, 304, 479
EaeI YGGCCR 1 cut(s) 75
EagI CGGCCG 1 cut(s) 75
Eam1104I CTCTTC 2 cut(s) 22, 174
EarI CTCTTC 2 cut(s) 22, 174
EclXI CGGCCG 1 cut(s) 75
Eco47I GGWCC 2 cut(s) 41, 488
Eco52I CGGCCG 1 cut(s) 75
Eco88I CYCGRG 1 cut(s) 244
EcoO109I RGGNCCY 1 cut(s) 41
FaeI CATG 2 cut(s) 201, 302
FaiI YATR 7 cut(s) 60, 199, 215, 300, 359, 369, 371
FaqI GGGAC 2 cut(s) 54, 74
FatI CATG 2 cut(s) 197, 298
FauNDI CATATG 1 cut(s) 369
Fnu4HI GCNGC 3 cut(s) 78, 192, 203
Fsp4HI GCNGC 3 cut(s) 78, 192, 203
GluI GCNGC 3 cut(s) 78, 192, 203
GsuI CTGGAG 1 cut(s) 395
HaeIII GGCC 2 cut(s) 77, 185
HapII CCGG 2 cut(s) 233, 263
HgaI GACGC 2 cut(s) 58, 164
Hin1I GRCGYC 1 cut(s) 50
Hin1II CATG 2 cut(s) 201, 302
HinfI GANTC 2 cut(s) 62, 229
HpaII CCGG 2 cut(s) 233, 263
HphI GGTGA 1 cut(s) 181
Hpy166II GTNNAC 1 cut(s) 145
Hpy188I TCNGA 3 cut(s) 139, 240, 484
Hpy188III TCNNGA 3 cut(s) 33, 152, 233
Hpy8I GTNNAC 1 cut(s) 145
Hpy99I CGWCG 4 cut(s) 31, 98, 125, 143
HpyAV CCTTC 2 cut(s) 64, 211
HpyCH4III ACNGT 2 cut(s) 128, 149
HpyCH4IV ACGT 3 cut(s) 7, 141, 493
HpyF10VI GCNNNNNNNGC 2 cut(s) 74, 191
HpyF3I CTNAG 2 cut(s) 85, 165
HpySE526I ACGT 3 cut(s) 7, 141, 493
Hsp92I GRCGYC 1 cut(s) 50
Hsp92II CATG 2 cut(s) 201, 302
KflI GGGWCCC 1 cut(s) 41
Kpn2I TCCGGA 1 cut(s) 232
Kzo9I GATC 3 cut(s) 235, 304, 479
LmnI GCTCC 1 cut(s) 210
LpnPI CCDG 5 cut(s) 23, 160, 246, 276, 359
Lsp1109I GCAGC 2 cut(s) 178, 189
MaeII ACGT 3 cut(s) 7, 141, 493
MaeIII GTNAC 2 cut(s) 46, 128
MalI GATC 3 cut(s) 237, 306, 481
MboI GATC 3 cut(s) 235, 304, 479
MboII GAAGA 2 cut(s) 9, 191
MflI RGATCY 2 cut(s) 235, 304
MluCI AATT 1 cut(s) 267
MlyI GAGTC 1 cut(s) 56
MmeI TCCRAC 2 cut(s) 35, 162
MroI TCCGGA 1 cut(s) 232
MroXI GAANNNNTTC 1 cut(s) 270
MspI CCGG 2 cut(s) 233, 263
MspR9I CCNGG 1 cut(s) 263
MwoI GCNNNNNNNGC 2 cut(s) 74, 191
NciI CCSGG 1 cut(s) 263
NdeI CATATG 1 cut(s) 369
NdeII GATC 3 cut(s) 235, 304, 479
NlaIII CATG 2 cut(s) 201, 302
NlaIV GGNNCC 3 cut(s) 42, 43, 306
NmeAIII GCCGAG 3 cut(s) 44, 53, 211
NmuCI GTSAC 1 cut(s) 46
NspI RCATGY 1 cut(s) 302
PdmI GAANNNNTTC 1 cut(s) 270
PfeI GAWTC 1 cut(s) 229
PflFI GACNNNGTC 1 cut(s) 492
PfoI TCCNGGA 1 cut(s) 261
PkrI GCNGC 3 cut(s) 79, 193, 204
PleI GAGTC 1 cut(s) 56
PpsI GAGTC 1 cut(s) 56
PpuMI RGGWCCY 1 cut(s) 41
Psp5II RGGWCCY 1 cut(s) 41
PspN4I GGNNCC 3 cut(s) 42, 43, 306
PspPI GGNCC 2 cut(s) 41, 488
PspPPI RGGWCCY 1 cut(s) 41
PstNI CAGNNNCTG 1 cut(s) 164
PsuI RGATCY 2 cut(s) 235, 304
PsyI GACNNNGTC 1 cut(s) 492
SatI GCNGC 3 cut(s) 78, 192, 203
Sau3AI GATC 3 cut(s) 235, 304, 479
Sau96I GGNCC 2 cut(s) 41, 488
SchI GAGTC 1 cut(s) 56
ScrFI CCNGG 1 cut(s) 263
SetI ASST 7 cut(s) 10, 17, 144, 171, 427, 449, 496
SinI GGWCC 2 cut(s) 41, 488
Sse9I AATT 1 cut(s) 267
SsiI CCGC 1 cut(s) 78
StyD4I CCNGG 1 cut(s) 261
TaaI ACNGT 2 cut(s) 128, 149
TaiI ACGT 3 cut(s) 10, 144, 496
TaqI TCGA 2 cut(s) 120, 472
TasI AATT 1 cut(s) 267
TauI GCSGC 1 cut(s) 80
TfiI GAWTC 1 cut(s) 229
TseFI GTSAC 1 cut(s) 46
TseI GCWGC 2 cut(s) 191, 202
Tsp45I GTSAC 1 cut(s) 46
TspDTI ATGAA 2 cut(s) 186, 230
TspGWI ACGGA 3 cut(s) 264, 297, 501
Tth111I GACNNNGTC 1 cut(s) 492
VpaK11BI GGWCC 2 cut(s) 41, 488
XceI RCATGY 1 cut(s) 302
XmnI GAANNNNTTC 1 cut(s) 270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.