Helicase protein
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1650 |
| AarI | CACCTGC | 3 cut(s) | 1896, 4951, 4987 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 2090 |
| AatII | GACGTC | 1 cut(s) | 2174 |
| Acc16I | TGCGCA | 2 cut(s) | 374, 896 |
| Acc36I | ACCTGC | 3 cut(s) | 1896, 4951, 4987 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 4843 |
| AccBSI | CCGCTC | 3 cut(s) | 385, 418, 736 |
| AccI | GTMKAC | 1 cut(s) | 1365 |
| AccII | CGCG | 1 cut(s) | 1866 |
| AciI | CCGC | 9 cut(s) | 49, 385, 416, 736, 743, 1453, 1864, 2456, 5051 |
| AclI | AACGTT | 1 cut(s) | 3057 |
| AcoI | YGGCCR | 4 cut(s) | 1758, 2956, 3169, 4905 |
| AcsI | RAATTY | 5 cut(s) | 1880, 2685, 4688, 4822, 5419 |
| AcyI | GRCGYC | 2 cut(s) | 53, 2171 |
| AdeI | CACNNNGTG | 1 cut(s) | 4484 |
| AfaI | GTAC | 9 cut(s) | 1483, 1569, 2392, 2595, 4018, 4176, 4878, 4999, 5493 |
| AflII | CTTAAG | 2 cut(s) | 746, 1811 |
| AflIII | ACRYGT | 3 cut(s) | 4361, 4873, 5479 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 57 |
| AhlI | ACTAGT | 3 cut(s) | 698, 2018, 4397 |
| AjnI | CCWGG | 8 cut(s) | 454, 607, 631, 689, 1186, 1946, 3930, 4755 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 1739, 1771, 5410, 5442 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 451, 483 |
| Alw21I | GWGCWC | 4 cut(s) | 2666, 2875, 3076, 5479 |
| Alw44I | GTGCAC | 3 cut(s) | 2871, 3072, 5475 |
| AlwNI | CAGNNNCTG | 4 cut(s) | 245, 2990, 4241, 4843 |
| Ama87I | CYCGRG | 2 cut(s) | 136, 2419 |
| ApaI | GGGCCC | 1 cut(s) | 5302 |
| ApaLI | GTGCAC | 3 cut(s) | 2871, 3072, 5475 |
| ApoI | RAATTY | 5 cut(s) | 1880, 2685, 4688, 4822, 5419 |
| ArsI | GACNNNNNNTTYG | 6 cut(s) | 3142, 3174, 3358, 3390, 3559, 3591 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 2685 |
| AspA2I | CCTAGG | 1 cut(s) | 3757 |
| AspLEI | GCGC | 3 cut(s) | 375, 564, 897 |
| AspS9I | GGNCC | 9 cut(s) | 1549, 1988, 2092, 2525, 3778, 4805, 4846, 5298, 5299 |
| AsuII | TTCGAA | 2 cut(s) | 3377, 3578 |
| AvaI | CYCGRG | 2 cut(s) | 136, 2419 |
| AvaII | GGWCC | 7 cut(s) | 1549, 1988, 2092, 2525, 3778, 4805, 4846 |
| AvrII | CCTAGG | 1 cut(s) | 3757 |
| BaeGI | GKGCMC | 6 cut(s) | 2502, 2875, 3076, 5113, 5302, 5479 |
| BalI | TGGCCA | 3 cut(s) | 2958, 3171, 4907 |
| BamHI | GGATCC | 6 cut(s) | 2785, 2980, 3093, 3286, 3409, 3610 |
| BanII | GRGCYC | 1 cut(s) | 5302 |
| BbsI | GAAGAC | 4 cut(s) | 1590, 2417, 2547, 2892 |
| Bbv12I | GWGCWC | 4 cut(s) | 2666, 2875, 3076, 5479 |
| BbvCI | CCTCAGC | 1 cut(s) | 3229 |
| BceAI | ACGGC | 3 cut(s) | 1745, 4619, 4877 |
| BciT130I | CCWGG | 8 cut(s) | 456, 609, 633, 691, 1188, 1948, 3932, 4757 |
| BciVI | GTATCC | 2 cut(s) | 4072, 4117 |
| BclI | TGATCA | 2 cut(s) | 2281, 3994 |
| BcuI | ACTAGT | 3 cut(s) | 698, 2018, 4397 |
| BfmI | CTRYAG | 8 cut(s) | 993, 3189, 3897, 3950, 4038, 4073, 4917, 4958 |
| BfrI | CTTAAG | 2 cut(s) | 746, 1811 |
| BfuAI | ACCTGC | 3 cut(s) | 1896, 4951, 4987 |
| BfuI | GTATCC | 2 cut(s) | 4072, 4117 |
| BglII | AGATCT | 1 cut(s) | 3193 |
| BlnI | CCTAGG | 1 cut(s) | 3757 |
| Bme1390I | CCNGG | 8 cut(s) | 456, 609, 633, 691, 1188, 1948, 3932, 4757 |
| Bme18I | GGWCC | 7 cut(s) | 1549, 1988, 2092, 2525, 3778, 4805, 4846 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 57 |
| BmeT110I | CYCGRG | 2 cut(s) | 136, 2419 |
| BmgT120I | GGNCC | 9 cut(s) | 1549, 1988, 2092, 2525, 3778, 4805, 4846, 5298, 5299 |
| BmrFI | CCNGG | 8 cut(s) | 456, 609, 633, 691, 1188, 1948, 3932, 4757 |
| BmrI | ACTGGG | 3 cut(s) | 2624, 3876, 5483 |
| BmuI | ACTGGG | 3 cut(s) | 2624, 3876, 5483 |
| BoxI | GACNNNNGTC | 1 cut(s) | 4647 |
| BpiI | GAAGAC | 4 cut(s) | 1590, 2417, 2547, 2892 |
| BpmI | CTGGAG | 3 cut(s) | 645, 2160, 4869 |
| Bpu10I | CCTNAGC | 4 cut(s) | 419, 1992, 3229, 4293 |
| Bpu14I | TTCGAA | 2 cut(s) | 3377, 3578 |
| BpuEI | CTTGAG | 7 cut(s) | 1895, 2100, 3167, 3383, 3512, 3584, 3752 |
| Bsa29I | ATCGAT | 1 cut(s) | 1152 |
| BsaAI | YACGTR | 1 cut(s) | 1264 |
| BsaHI | GRCGYC | 2 cut(s) | 53, 2171 |
| BsaI | GGTCTC | 1 cut(s) | 3452 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 639, 669, 972, 1002 |
| Bse3DI | GCAATG | 3 cut(s) | 1304, 2570, 4052 |
| BseBI | CCWGG | 8 cut(s) | 456, 609, 633, 691, 1188, 1948, 3932, 4757 |
| BseCI | ATCGAT | 1 cut(s) | 1152 |
| BseMI | GCAATG | 3 cut(s) | 1304, 2570, 4052 |
| BseRI | GAGGAG | 4 cut(s) | 82, 993, 1148, 4643 |
| BseSI | GKGCMC | 6 cut(s) | 2502, 2875, 3076, 5113, 5302, 5479 |
| BseYI | CCCAGC | 3 cut(s) | 4313, 4952, 5113 |
| BsgI | GTGCAG | 1 cut(s) | 4461 |
| Bsh1236I | CGCG | 1 cut(s) | 1866 |
| Bsh1285I | CGRYCG | 1 cut(s) | 5377 |
| BshVI | ATCGAT | 1 cut(s) | 1152 |
| BsiEI | CGRYCG | 1 cut(s) | 5377 |
| BsiHKAI | GWGCWC | 4 cut(s) | 2666, 2875, 3076, 5479 |
| BsiHKCI | CYCGRG | 2 cut(s) | 136, 2419 |
| BsiSI | CCGG | 1 cut(s) | 4157 |
| BslFI | GGGAC | 5 cut(s) | 45, 2239, 4449, 4498, 4738 |
| BsmBI | CGTCTC | 1 cut(s) | 2178 |
| BsmFI | GGGAC | 5 cut(s) | 45, 2239, 4449, 4498, 4738 |
| BsmI | GAATGC | 4 cut(s) | 2447, 3732, 4416, 5037 |
| Bso31I | GGTCTC | 1 cut(s) | 3452 |
| BsoBI | CYCGRG | 2 cut(s) | 136, 2419 |
| Bsp119I | TTCGAA | 2 cut(s) | 3377, 3578 |
| Bsp120I | GGGCCC | 1 cut(s) | 5298 |
| Bsp1286I | GDGCHC | 7 cut(s) | 2502, 2666, 2875, 3076, 5113, 5302, 5479 |
| Bsp1407I | TGTACA | 3 cut(s) | 4174, 4876, 4997 |
| BspACI | CCGC | 9 cut(s) | 49, 385, 416, 736, 743, 1453, 1864, 2456, 5051 |
| BspDI | ATCGAT | 1 cut(s) | 1152 |
| BspFNI | CGCG | 1 cut(s) | 1866 |
| BspHI | TCATGA | 2 cut(s) | 4147, 4579 |
| BspMAI | CTGCAG | 7 cut(s) | 997, 3901, 3954, 4042, 4077, 4921, 4962 |
| BspMI | ACCTGC | 3 cut(s) | 1896, 4951, 4987 |
| BspQI | GCTCTTC | 2 cut(s) | 1830, 2988 |
| BspT104I | TTCGAA | 2 cut(s) | 3377, 3578 |
| BspTI | CTTAAG | 2 cut(s) | 746, 1811 |
| BspTNI | GGTCTC | 1 cut(s) | 3452 |
| BsrBI | CCGCTC | 3 cut(s) | 385, 418, 736 |
| BsrDI | GCAATG | 3 cut(s) | 1304, 2570, 4052 |
| BsrGI | TGTACA | 3 cut(s) | 4174, 4876, 4997 |
| BssNI | GRCGYC | 2 cut(s) | 53, 2171 |
| BssT1I | CCWWGG | 6 cut(s) | 1997, 2764, 2959, 3172, 3757, 5089 |
| Bst2UI | CCWGG | 8 cut(s) | 456, 609, 633, 691, 1188, 1948, 3932, 4757 |
| Bst6I | CTCTTC | 8 cut(s) | 173, 507, 1419, 1830, 2988, 3919, 4530, 5077 |
| BstACI | GRCGYC | 2 cut(s) | 53, 2171 |
| BstAFI | CTTAAG | 2 cut(s) | 746, 1811 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 245, 4796 |
| BstAUI | TGTACA | 3 cut(s) | 4174, 4876, 4997 |
| BstBAI | YACGTR | 1 cut(s) | 1264 |
| BstBI | TTCGAA | 2 cut(s) | 3377, 3578 |
| BstDSI | CCRYGG | 1 cut(s) | 4483 |
| BstEII | GGTNACC | 3 cut(s) | 1974, 3325, 4963 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 2133 |
| BstFNI | CGCG | 1 cut(s) | 1866 |
| BstHHI | GCGC | 3 cut(s) | 375, 564, 897 |
| BstMCI | CGRYCG | 1 cut(s) | 5377 |
| BstNI | CCWGG | 8 cut(s) | 456, 609, 633, 691, 1188, 1948, 3932, 4757 |
| BstNSI | RCATGY | 6 cut(s) | 1562, 3317, 3845, 4365, 4877, 5483 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 4647 |
| BstPI | GGTNACC | 3 cut(s) | 1974, 3325, 4963 |
| BstSCI | CCNGG | 8 cut(s) | 454, 607, 631, 689, 1186, 1946, 3930, 4755 |
| BstSFI | CTRYAG | 8 cut(s) | 993, 3189, 3897, 3950, 4038, 4073, 4917, 4958 |
| BstSLI | GKGCMC | 6 cut(s) | 2502, 2875, 3076, 5113, 5302, 5479 |
| BstSNI | TACGTA | 1 cut(s) | 1264 |
| BstUI | CGCG | 1 cut(s) | 1866 |
| BstV2I | GAAGAC | 4 cut(s) | 1590, 2417, 2547, 2892 |
| BstX2I | RGATCY | 9 cut(s) | 2785, 2980, 3093, 3193, 3286, 3409, 3610, 4054, 5504 |
| BstYI | RGATCY | 9 cut(s) | 2785, 2980, 3093, 3193, 3286, 3409, 3610, 4054, 5504 |
| Bsu15I | ATCGAT | 1 cut(s) | 1152 |
| BsuI | GTATCC | 2 cut(s) | 4072, 4117 |
| BsuTUI | ATCGAT | 1 cut(s) | 1152 |
| BtgI | CCRYGG | 1 cut(s) | 4483 |
| BtsI | GCAGTG | 7 cut(s) | 1509, 2700, 3324, 3693, 3838, 4047, 5348 |
| BveI | ACCTGC | 3 cut(s) | 1896, 4951, 4987 |
| CaiI | CAGNNNCTG | 4 cut(s) | 245, 2990, 4241, 4843 |
| CciI | TCATGA | 2 cut(s) | 4147, 4579 |
| CfoI | GCGC | 3 cut(s) | 375, 564, 897 |
| Cfr13I | GGNCC | 9 cut(s) | 1549, 1988, 2092, 2525, 3778, 4805, 4846, 5298, 5299 |
| ClaI | ATCGAT | 1 cut(s) | 1152 |
| CseI | GACGC | 2 cut(s) | 61, 4586 |
| Csp6I | GTAC | 9 cut(s) | 1482, 1568, 2391, 2594, 4017, 4175, 4877, 4998, 5492 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 2601, 2636, 4099, 4134 |
| CviQI | GTAC | 9 cut(s) | 1482, 1568, 2391, 2594, 4017, 4175, 4877, 4998, 5492 |
| DraIII | CACNNNGTG | 1 cut(s) | 4484 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 2090 |
| DriI | GACNNNNNGTC | 1 cut(s) | 57 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 2090 |
| EaeI | YGGCCR | 4 cut(s) | 1758, 2956, 3169, 4905 |
| Eam1104I | CTCTTC | 8 cut(s) | 173, 507, 1419, 1830, 2988, 3919, 4530, 5077 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 57 |
| EarI | CTCTTC | 8 cut(s) | 173, 507, 1419, 1830, 2988, 3919, 4530, 5077 |
| Eco105I | TACGTA | 1 cut(s) | 1264 |
| Eco130I | CCWWGG | 6 cut(s) | 1997, 2764, 2959, 3172, 3757, 5089 |
| Eco24I | GRGCYC | 1 cut(s) | 5302 |
| Eco31I | GGTCTC | 1 cut(s) | 3452 |
| Eco32I | GATATC | 1 cut(s) | 1194 |
| Eco47I | GGWCC | 7 cut(s) | 1549, 1988, 2092, 2525, 3778, 4805, 4846 |
| Eco88I | CYCGRG | 2 cut(s) | 136, 2419 |
| Eco91I | GGTNACC | 3 cut(s) | 1974, 3325, 4963 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 2133 |
| EcoO109I | RGGNCCY | 1 cut(s) | 5298 |
| EcoO65I | GGTNACC | 3 cut(s) | 1974, 3325, 4963 |
| EcoRII | CCWGG | 8 cut(s) | 454, 607, 631, 689, 1186, 1946, 3930, 4755 |
| EcoRV | GATATC | 1 cut(s) | 1194 |
| EcoT14I | CCWWGG | 6 cut(s) | 1997, 2764, 2959, 3172, 3757, 5089 |
| EcoT22I | ATGCAT | 2 cut(s) | 2447, 5230 |
| EcoT38I | GRGCYC | 1 cut(s) | 5302 |
| ErhI | CCWWGG | 6 cut(s) | 1997, 2764, 2959, 3172, 3757, 5089 |
| Esp3I | CGTCTC | 1 cut(s) | 2178 |
| FaqI | GGGAC | 5 cut(s) | 45, 2239, 4449, 4498, 4738 |
| FauI | CCCGC | 2 cut(s) | 378, 729 |
| FauNDI | CATATG | 2 cut(s) | 1851, 3235 |
| FbaI | TGATCA | 2 cut(s) | 2281, 3994 |
| FblI | GTMKAC | 1 cut(s) | 1365 |
| FriOI | GRGCYC | 1 cut(s) | 5302 |
| FspI | TGCGCA | 2 cut(s) | 374, 896 |
| GlaI | GCGC | 3 cut(s) | 374, 563, 896 |
| GsaI | CCCAGC | 3 cut(s) | 4317, 4956, 5117 |
| GsuI | CTGGAG | 3 cut(s) | 645, 2160, 4869 |
| HapII | CCGG | 1 cut(s) | 4157 |
| HgaI | GACGC | 2 cut(s) | 61, 4586 |
| HhaI | GCGC | 3 cut(s) | 375, 564, 897 |
| Hin1I | GRCGYC | 2 cut(s) | 53, 2171 |
| Hin6I | GCGC | 3 cut(s) | 373, 562, 895 |
| HinP1I | GCGC | 3 cut(s) | 373, 562, 895 |
| HincII | GTYRAC | 3 cut(s) | 696, 1366, 5485 |
| HindII | GTYRAC | 3 cut(s) | 696, 1366, 5485 |
| HpaII | CCGG | 1 cut(s) | 4157 |
| Hpy99I | CGWCG | 3 cut(s) | 3278, 4653, 5516 |
| HpyCH4IV | ACGT | 6 cut(s) | 1263, 1938, 2171, 3057, 3273, 5514 |
| HpySE526I | ACGT | 6 cut(s) | 1263, 1938, 2171, 3057, 3273, 5514 |
| Hsp92I | GRCGYC | 2 cut(s) | 53, 2171 |
| HspAI | GCGC | 3 cut(s) | 373, 562, 895 |
| Ksp22I | TGATCA | 2 cut(s) | 2281, 3994 |
| LguI | GCTCTTC | 2 cut(s) | 1830, 2988 |
| LmnI | GCTCC | 7 cut(s) | 647, 980, 1912, 2131, 2614, 4303, 5029 |
| MaeII | ACGT | 6 cut(s) | 1263, 1938, 2171, 3057, 3273, 5514 |
| MbiI | CCGCTC | 3 cut(s) | 385, 418, 736 |
| MfeI | CAATTG | 2 cut(s) | 503, 714 |
| MflI | RGATCY | 9 cut(s) | 2785, 2980, 3093, 3193, 3286, 3409, 3610, 4054, 5504 |
| MhlI | GDGCHC | 7 cut(s) | 2502, 2666, 2875, 3076, 5113, 5302, 5479 |
| MlsI | TGGCCA | 3 cut(s) | 2958, 3171, 4907 |
| MluNI | TGGCCA | 3 cut(s) | 2958, 3171, 4907 |
| MlyI | GAGTC | 6 cut(s) | 67, 1061, 1218, 2431, 3251, 3503 |
| MmeI | TCCRAC | 7 cut(s) | 420, 747, 1313, 1800, 4527, 5152, 5194 |
| Mox20I | TGGCCA | 3 cut(s) | 2958, 3171, 4907 |
| Mph1103I | ATGCAT | 2 cut(s) | 2447, 5230 |
| MroXI | GAANNNNTTC | 1 cut(s) | 2685 |
| MscI | TGGCCA | 3 cut(s) | 2958, 3171, 4907 |
| MslI | CAYNNNNRTG | 3 cut(s) | 402, 1557, 2628 |
| Msp20I | TGGCCA | 3 cut(s) | 2958, 3171, 4907 |
| MspCI | CTTAAG | 2 cut(s) | 746, 1811 |
| MspI | CCGG | 1 cut(s) | 4157 |
| MspR9I | CCNGG | 8 cut(s) | 456, 609, 633, 691, 1188, 1948, 3932, 4757 |
| MunI | CAATTG | 2 cut(s) | 503, 714 |
| Mva1269I | GAATGC | 4 cut(s) | 2447, 3732, 4416, 5037 |
| MvaI | CCWGG | 8 cut(s) | 456, 609, 633, 691, 1188, 1948, 3932, 4757 |
| MvnI | CGCG | 1 cut(s) | 1866 |
| NdeI | CATATG | 2 cut(s) | 1851, 3235 |
| NmeAIII | GCCGAG | 1 cut(s) | 81 |
| NsbI | TGCGCA | 2 cut(s) | 374, 896 |
| NsiI | ATGCAT | 2 cut(s) | 2447, 5230 |
| NspI | RCATGY | 6 cut(s) | 1562, 3317, 3845, 4365, 4877, 5483 |
| NspV | TTCGAA | 2 cut(s) | 3377, 3578 |
| PagI | TCATGA | 2 cut(s) | 4147, 4579 |
| PaqCI | CACCTGC | 3 cut(s) | 1896, 4951, 4987 |
| PciI | ACATGT | 3 cut(s) | 4361, 4873, 5479 |
| PciSI | GCTCTTC | 2 cut(s) | 1830, 2988 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 143 |
| PctI | GAATGC | 4 cut(s) | 2447, 3732, 4416, 5037 |
| PdmI | GAANNNNTTC | 1 cut(s) | 2685 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 4843 |
| PfoI | TCCNGGA | 1 cut(s) | 631 |
| PleI | GAGTC | 6 cut(s) | 66, 1061, 1217, 2430, 3250, 3502 |
| PpsI | GAGTC | 6 cut(s) | 66, 1061, 1217, 2430, 3250, 3502 |
| Ppu21I | YACGTR | 1 cut(s) | 1264 |
| PscI | ACATGT | 3 cut(s) | 4361, 4873, 5479 |
| PshAI | GACNNNNGTC | 1 cut(s) | 4647 |
| PsiI | TTATAA | 1 cut(s) | 1650 |
| Psp1406I | AACGTT | 1 cut(s) | 3057 |
| Psp6I | CCWGG | 8 cut(s) | 454, 607, 631, 689, 1186, 1946, 3930, 4755 |
| PspEI | GGTNACC | 3 cut(s) | 1974, 3325, 4963 |
| PspFI | CCCAGC | 3 cut(s) | 4313, 4952, 5113 |
| PspGI | CCWGG | 8 cut(s) | 454, 607, 631, 689, 1186, 1946, 3930, 4755 |
| PspOMI | GGGCCC | 1 cut(s) | 5298 |
| PspPI | GGNCC | 9 cut(s) | 1549, 1988, 2092, 2525, 3778, 4805, 4846, 5298, 5299 |
| PstI | CTGCAG | 7 cut(s) | 997, 3901, 3954, 4042, 4077, 4921, 4962 |
| PstNI | CAGNNNCTG | 4 cut(s) | 245, 2990, 4241, 4843 |
| PsuI | RGATCY | 9 cut(s) | 2785, 2980, 3093, 3193, 3286, 3409, 3610, 4054, 5504 |
| RsaI | GTAC | 9 cut(s) | 1483, 1569, 2392, 2595, 4018, 4176, 4878, 4999, 5493 |
| RsaNI | GTAC | 9 cut(s) | 1482, 1568, 2391, 2594, 4017, 4175, 4877, 4998, 5492 |
| RseI | CAYNNNNRTG | 3 cut(s) | 402, 1557, 2628 |
| SalI | GTCGAC | 1 cut(s) | 1364 |
| SapI | GCTCTTC | 2 cut(s) | 1830, 2988 |
| Sau96I | GGNCC | 9 cut(s) | 1549, 1988, 2092, 2525, 3778, 4805, 4846, 5298, 5299 |
| SbfI | CCTGCAGG | 1 cut(s) | 4962 |
| SchI | GAGTC | 6 cut(s) | 67, 1061, 1218, 2431, 3251, 3503 |
| ScrFI | CCNGG | 8 cut(s) | 456, 609, 633, 691, 1188, 1948, 3932, 4757 |
| SdaI | CCTGCAGG | 1 cut(s) | 4962 |
| SduI | GDGCHC | 7 cut(s) | 2502, 2666, 2875, 3076, 5113, 5302, 5479 |
| SfcI | CTRYAG | 8 cut(s) | 993, 3189, 3897, 3950, 4038, 4073, 4917, 4958 |
| SfuI | TTCGAA | 2 cut(s) | 3377, 3578 |
| SinI | GGWCC | 7 cut(s) | 1549, 1988, 2092, 2525, 3778, 4805, 4846 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 402, 1557, 2628 |
| SmlI | CTYRAG | 9 cut(s) | 746, 1811, 1874, 2115, 3146, 3362, 3491, 3563, 3731 |
| SmoI | CTYRAG | 9 cut(s) | 746, 1811, 1874, 2115, 3146, 3362, 3491, 3563, 3731 |
| SnaBI | TACGTA | 1 cut(s) | 1264 |
| SpeI | ACTAGT | 3 cut(s) | 698, 2018, 4397 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 4962 |
| SsiI | CCGC | 9 cut(s) | 49, 385, 416, 736, 743, 1453, 1864, 2456, 5051 |
| SspI | AATATT | 2 cut(s) | 1607, 2650 |
| StyD4I | CCNGG | 8 cut(s) | 454, 607, 631, 689, 1186, 1946, 3930, 4755 |
| StyI | CCWWGG | 6 cut(s) | 1997, 2764, 2959, 3172, 3757, 5089 |
| TaiI | ACGT | 6 cut(s) | 1266, 1941, 2174, 3060, 3276, 5517 |
| TatI | WGTACW | 4 cut(s) | 2390, 4174, 4876, 4997 |
| TauI | GCSGC | 1 cut(s) | 418 |
| TspGWI | ACGGA | 3 cut(s) | 354, 416, 2298 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 4843 |
| Vha464I | CTTAAG | 2 cut(s) | 746, 1811 |
| VneI | GTGCAC | 3 cut(s) | 2871, 3072, 5475 |
| VpaK11BI | GGWCC | 7 cut(s) | 1549, 1988, 2092, 2525, 3778, 4805, 4846 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 2133 |
| XapI | RAATTY | 5 cut(s) | 1880, 2685, 4688, 4822, 5419 |
| XbaI | TCTAGA | 1 cut(s) | 1600 |
| XceI | RCATGY | 6 cut(s) | 1562, 3317, 3845, 4365, 4877, 5483 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 4900 |
| XmaJI | CCTAGG | 1 cut(s) | 3757 |
| XmiI | GTMKAC | 1 cut(s) | 1365 |
| XmnI | GAANNNNTTC | 1 cut(s) | 2685 |
| ZraI | GACGTC | 1 cut(s) | 2172 |
| Zsp2I | ATGCAT | 2 cut(s) | 2447, 5230 |