helicase protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 591 |
| Acc36I | ACCTGC | 1 cut(s) | 5797 |
| Acc65I | GGTACC | 2 cut(s) | 4541, 5894 |
| AccB1I | GGYRCC | 4 cut(s) | 528, 4541, 5894, 5993 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 4426, 5539 |
| AccI | GTMKAC | 5 cut(s) | 860, 915, 1276, 3021, 4974 |
| AciI | CCGC | 9 cut(s) | 1635, 1815, 2199, 3086, 3109, 3136, 4280, 5049, 5499 |
| AclI | AACGTT | 3 cut(s) | 571, 3125, 3813 |
| AclWI | GGATC | 9 cut(s) | 90, 103, 790, 1196, 1488, 3734, 5210, 5484, 6269 |
| AcoI | YGGCCR | 2 cut(s) | 2589, 4961 |
| AcvI | CACGTG | 1 cut(s) | 3044 |
| AcyI | GRCGYC | 2 cut(s) | 529, 668 |
| AdeI | CACNNNGTG | 1 cut(s) | 3502 |
| AfiI | CCNNNNNNNGG | 9 cut(s) | 1001, 4426, 5105, 5388, 5539, 5891, 5927, 6122, 6123 |
| AflII | CTTAAG | 3 cut(s) | 136, 3551, 5480 |
| AflIII | ACRYGT | 3 cut(s) | 2775, 4832, 6133 |
| AjnI | CCWGG | 6 cut(s) | 469, 1352, 2250, 2602, 4201, 5178 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1595, 1627 |
| Alw21I | GWGCWC | 6 cut(s) | 1477, 2152, 3736, 4413, 5701, 5761 |
| Alw26I | GTCTC | 7 cut(s) | 844, 1877, 3271, 4103, 4454, 5277, 5351 |
| Alw44I | GTGCAC | 4 cut(s) | 2148, 3732, 5697, 5757 |
| AlwI | GGATC | 9 cut(s) | 90, 103, 790, 1196, 1488, 3734, 5210, 5484, 6269 |
| AlwNI | CAGNNNCTG | 4 cut(s) | 738, 1340, 4499, 5539 |
| Ama87I | CYCGRG | 4 cut(s) | 283, 1307, 3524, 4706 |
| AoxI | GGCC | 8 cut(s) | 594, 1567, 2589, 4961, 5573, 5972, 6049, 6126 |
| ApaI | GGGCCC | 1 cut(s) | 6130 |
| ApaLI | GTGCAC | 4 cut(s) | 2148, 3732, 5697, 5757 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 4102, 4134 |
| AseI | ATTAAT | 1 cut(s) | 612 |
| Asp700I | GAANNNNTTC | 8 cut(s) | 703, 1569, 2085, 2177, 2907, 3296, 3427, 3862 |
| Asp718I | GGTACC | 2 cut(s) | 4541, 5894 |
| AspLEI | GCGC | 2 cut(s) | 19, 531 |
| AspS9I | GGNCC | 6 cut(s) | 300, 467, 3088, 6050, 6126, 6127 |
| AsuC2I | CCSGG | 1 cut(s) | 3896 |
| AsuII | TTCGAA | 2 cut(s) | 1590, 4244 |
| AsuNHI | GCTAGC | 2 cut(s) | 1631, 5618 |
| AvaI | CYCGRG | 4 cut(s) | 283, 1307, 3524, 4706 |
| AvaII | GGWCC | 3 cut(s) | 300, 467, 3088 |
| BaeGI | GKGCMC | 6 cut(s) | 2152, 3736, 5701, 5761, 5947, 6130 |
| BaeI | ACNNNNGTAYC | 6 cut(s) | 43, 76, 117, 150, 5878, 5911 |
| BamHI | GGATCC | 1 cut(s) | 95 |
| BanI | GGYRCC | 4 cut(s) | 528, 4541, 5894, 5993 |
| BanII | GRGCYC | 3 cut(s) | 188, 4011, 6130 |
| BarI | GAAGNNNNNNTAC | 4 cut(s) | 733, 765, 1524, 1556 |
| BauI | CACGAG | 2 cut(s) | 501, 1887 |
| BbrPI | CACGTG | 1 cut(s) | 3044 |
| BbsI | GAAGAC | 4 cut(s) | 138, 1785, 3240, 3926 |
| Bbv12I | GWGCWC | 6 cut(s) | 1477, 2152, 3736, 4413, 5701, 5761 |
| BbvCI | CCTCAGC | 2 cut(s) | 5199, 5451 |
| BceAI | ACGGC | 1 cut(s) | 6064 |
| BciT130I | CCWGG | 6 cut(s) | 471, 1354, 2252, 2604, 4203, 5180 |
| BciVI | GTATCC | 2 cut(s) | 118, 4867 |
| BclI | TGATCA | 4 cut(s) | 1513, 4138, 4669, 4885 |
| BcnI | CCSGG | 1 cut(s) | 3896 |
| BcoDI | GTCTC | 7 cut(s) | 844, 1877, 3271, 4103, 4454, 5277, 5351 |
| BfoI | RGCGCY | 1 cut(s) | 532 |
| BfrI | CTTAAG | 3 cut(s) | 136, 3551, 5480 |
| BfuAI | ACCTGC | 1 cut(s) | 5797 |
| BfuI | GTATCC | 2 cut(s) | 118, 4867 |
| BglII | AGATCT | 2 cut(s) | 1462, 2881 |
| BlpI | GCTNAGC | 1 cut(s) | 3048 |
| BmcAI | AGTACT | 3 cut(s) | 196, 1367, 3769 |
| Bme1390I | CCNGG | 7 cut(s) | 471, 1354, 2252, 2604, 3896, 4203, 5180 |
| Bme18I | GGWCC | 3 cut(s) | 300, 467, 3088 |
| BmeT110I | CYCGRG | 4 cut(s) | 283, 1307, 3524, 4706 |
| BmgT120I | GGNCC | 6 cut(s) | 300, 467, 3088, 6050, 6126, 6127 |
| BmrFI | CCNGG | 7 cut(s) | 471, 1354, 2252, 2604, 3896, 4203, 5180 |
| BmrI | ACTGGG | 6 cut(s) | 1378, 1952, 3780, 4344, 4827, 6302 |
| BmtI | GCTAGC | 2 cut(s) | 1635, 5622 |
| BmuI | ACTGGG | 6 cut(s) | 1378, 1952, 3780, 4344, 4827, 6302 |
| BoxI | GACNNNNGTC | 2 cut(s) | 1300, 4473 |
| BpiI | GAAGAC | 4 cut(s) | 138, 1785, 3240, 3926 |
| BpmI | CTGGAG | 4 cut(s) | 816, 2355, 2640, 3690 |
| Bpu10I | CCTNAGC | 2 cut(s) | 5199, 5451 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 3048 |
| Bpu14I | TTCGAA | 2 cut(s) | 1590, 4244 |
| BpuEI | CTTGAG | 1 cut(s) | 236 |
| BpuMI | CCSGG | 1 cut(s) | 3896 |
| Bsa29I | ATCGAT | 1 cut(s) | 6223 |
| BsaAI | YACGTR | 1 cut(s) | 3044 |
| BsaHI | GRCGYC | 2 cut(s) | 529, 668 |
| BsaI | GGTCTC | 1 cut(s) | 4103 |
| BsaJI | CCNNGG | 7 cut(s) | 470, 523, 651, 4455, 5178, 5179, 5923 |
| BsaWI | WCCGGW | 2 cut(s) | 241, 3929 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 2634, 2664 |
| Bsc4I | CCNNNNNNNGG | 9 cut(s) | 1001, 4426, 5105, 5388, 5539, 5891, 5927, 6122, 6123 |
| Bse3DI | GCAATG | 4 cut(s) | 1614, 1839, 2182, 5357 |
| BseBI | CCWGG | 6 cut(s) | 471, 1354, 2252, 2604, 4203, 5180 |
| BseCI | ATCGAT | 1 cut(s) | 6223 |
| BseDI | CCNNGG | 7 cut(s) | 470, 523, 651, 4455, 5178, 5179, 5923 |
| BseLI | CCNNNNNNNGG | 9 cut(s) | 1001, 4426, 5105, 5388, 5539, 5891, 5927, 6122, 6123 |
| BseMI | GCAATG | 4 cut(s) | 1614, 1839, 2182, 5357 |
| BseRI | GAGGAG | 9 cut(s) | 103, 228, 2066, 2352, 2522, 3383, 4470, 5339, 6153 |
| BseSI | GKGCMC | 6 cut(s) | 2152, 3736, 5701, 5761, 5947, 6130 |
| BseYI | CCCAGC | 7 cut(s) | 5648, 5678, 5708, 5738, 5768, 5947, 6009 |
| BsgI | GTGCAG | 4 cut(s) | 805, 3459, 5046, 6165 |
| BshFI | GGCC | 8 cut(s) | 596, 1569, 2591, 4963, 5575, 5974, 6051, 6128 |
| BshNI | GGYRCC | 4 cut(s) | 528, 4541, 5894, 5993 |
| BshVI | ATCGAT | 1 cut(s) | 6223 |
| BsiHKAI | GWGCWC | 6 cut(s) | 1477, 2152, 3736, 4413, 5701, 5761 |
| BsiHKCI | CYCGRG | 4 cut(s) | 283, 1307, 3524, 4706 |
| BsiSI | CCGG | 4 cut(s) | 242, 995, 3896, 3930 |
| BslFI | GGGAC | 6 cut(s) | 2248, 2455, 3878, 4618, 5197, 5992 |
| BslI | CCNNNNNNNGG | 9 cut(s) | 1001, 4426, 5105, 5388, 5539, 5891, 5927, 6122, 6123 |
| BsmAI | GTCTC | 7 cut(s) | 844, 1877, 3271, 4103, 4454, 5277, 5351 |
| BsmFI | GGGAC | 6 cut(s) | 2248, 2455, 3878, 4618, 5197, 5992 |
| BsmI | GAATGC | 4 cut(s) | 1219, 1318, 4909, 5203 |
| BsnI | GGCC | 8 cut(s) | 596, 1569, 2591, 4963, 5575, 5974, 6051, 6128 |
| Bso31I | GGTCTC | 1 cut(s) | 4103 |
| BsoBI | CYCGRG | 4 cut(s) | 283, 1307, 3524, 4706 |
| Bsp119I | TTCGAA | 2 cut(s) | 1590, 4244 |
| Bsp120I | GGGCCC | 1 cut(s) | 6126 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 3048 |
| Bsp19I | CCATGG | 1 cut(s) | 651 |
| BspACI | CCGC | 9 cut(s) | 1635, 1815, 2199, 3086, 3109, 3136, 4280, 5049, 5499 |
| BspANI | GGCC | 8 cut(s) | 596, 1569, 2591, 4963, 5575, 5974, 6051, 6128 |
| BspDI | ATCGAT | 1 cut(s) | 6223 |
| BspHI | TCATGA | 4 cut(s) | 2922, 4672, 4888, 5275 |
| BspMAI | CTGCAG | 9 cut(s) | 1081, 3160, 4288, 4942, 5508, 5617, 5628, 5688, 5748 |
| BspMI | ACCTGC | 1 cut(s) | 5797 |
| BspOI | GCTAGC | 2 cut(s) | 1635, 5622 |
| BspPI | GGATC | 9 cut(s) | 90, 103, 790, 1196, 1488, 3734, 5210, 5484, 6269 |
| BspQI | GCTCTTC | 2 cut(s) | 3480, 3999 |
| BspT104I | TTCGAA | 2 cut(s) | 1590, 4244 |
| BspT107I | GGYRCC | 4 cut(s) | 528, 4541, 5894, 5993 |
| BspTI | CTTAAG | 3 cut(s) | 136, 3551, 5480 |
| BspTNI | GGTCTC | 1 cut(s) | 4103 |
| BsrDI | GCAATG | 4 cut(s) | 1614, 1839, 2182, 5357 |
| BssECI | CCNNGG | 7 cut(s) | 470, 523, 651, 4455, 5178, 5179, 5923 |
| BssNAI | GTATAC | 1 cut(s) | 1277 |
| BssNI | GRCGYC | 2 cut(s) | 529, 668 |
| BssSI | CACGAG | 2 cut(s) | 501, 1887 |
| BssT1I | CCWWGG | 4 cut(s) | 523, 651, 4455, 5923 |
| Bst1107I | GTATAC | 1 cut(s) | 1277 |
| Bst2BI | CACGAG | 2 cut(s) | 501, 1887 |
| Bst2UI | CCWGG | 6 cut(s) | 471, 1354, 2252, 2604, 4203, 5180 |
| Bst6I | CTCTTC | 7 cut(s) | 398, 600, 2683, 3075, 3480, 3999, 4671 |
| BstACI | GRCGYC | 2 cut(s) | 529, 668 |
| BstAFI | CTTAAG | 3 cut(s) | 136, 3551, 5480 |
| BstAPI | GCANNNNNTGC | 5 cut(s) | 3731, 3815, 4229, 5492, 6293 |
| BstBAI | YACGTR | 1 cut(s) | 3044 |
| BstBI | TTCGAA | 2 cut(s) | 1590, 4244 |
| BstDSI | CCRYGG | 1 cut(s) | 651 |
| BstH2I | RGCGCY | 1 cut(s) | 532 |
| BstHHI | GCGC | 2 cut(s) | 19, 531 |
| BstMAI | GTCTC | 7 cut(s) | 844, 1877, 3271, 4103, 4454, 5277, 5351 |
| BstNI | CCWGG | 6 cut(s) | 471, 1354, 2252, 2604, 4203, 5180 |
| BstNSI | RCATGY | 6 cut(s) | 1122, 2779, 4836, 5171, 5499, 6137 |
| BstPAI | GACNNNNGTC | 2 cut(s) | 1300, 4473 |
| BstSCI | CCNGG | 7 cut(s) | 469, 1352, 2250, 2602, 3894, 4201, 5178 |
| BstSLI | GKGCMC | 6 cut(s) | 2152, 3736, 5701, 5761, 5947, 6130 |
| BstV2I | GAAGAC | 4 cut(s) | 138, 1785, 3240, 3926 |
| BstX2I | RGATCY | 7 cut(s) | 95, 782, 1201, 1462, 1493, 2881, 5476 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 4817, 6017 |
| BstYI | RGATCY | 7 cut(s) | 95, 782, 1201, 1462, 1493, 2881, 5476 |
| BstZ17I | GTATAC | 1 cut(s) | 1277 |
| Bsu15I | ATCGAT | 1 cut(s) | 6223 |
| BsuI | GTATCC | 2 cut(s) | 118, 4867 |
| BsuRI | GGCC | 8 cut(s) | 596, 1569, 2591, 4963, 5575, 5974, 6051, 6128 |
| BsuTUI | ATCGAT | 1 cut(s) | 6223 |
| BtgI | CCRYGG | 1 cut(s) | 651 |
| BtsI | GCAGTG | 4 cut(s) | 739, 4230, 5006, 6282 |
| BveI | ACCTGC | 1 cut(s) | 5797 |
| CaiI | CAGNNNCTG | 4 cut(s) | 738, 1340, 4499, 5539 |
| CciI | TCATGA | 4 cut(s) | 2922, 4672, 4888, 5275 |
| CfoI | GCGC | 2 cut(s) | 19, 531 |
| Cfr13I | GGNCC | 6 cut(s) | 300, 467, 3088, 6050, 6126, 6127 |
| ClaI | ATCGAT | 1 cut(s) | 6223 |
| CpoI | CGGWCCG | 1 cut(s) | 3088 |
| CseI | GACGC | 5 cut(s) | 676, 1751, 4134, 4620, 5282 |
| CsiI | ACCWGGT | 1 cut(s) | 4201 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 4083, 4118 |
| CspI | CGGWCCG | 1 cut(s) | 3088 |
| DinI | GGCGCC | 1 cut(s) | 530 |
| DraIII | CACNNNGTG | 1 cut(s) | 3502 |
| EaeI | YGGCCR | 2 cut(s) | 2589, 4961 |
| Eam1104I | CTCTTC | 7 cut(s) | 398, 600, 2683, 3075, 3480, 3999, 4671 |
| EarI | CTCTTC | 7 cut(s) | 398, 600, 2683, 3075, 3480, 3999, 4671 |
| EciI | GGCGGA | 2 cut(s) | 3101, 4269 |
| Eco130I | CCWWGG | 4 cut(s) | 523, 651, 4455, 5923 |
| Eco147I | AGGCCT | 3 cut(s) | 1569, 5575, 5974 |
| Eco24I | GRGCYC | 3 cut(s) | 188, 4011, 6130 |
| Eco31I | GGTCTC | 1 cut(s) | 4103 |
| Eco32I | GATATC | 4 cut(s) | 1386, 1989, 2850, 2979 |
| Eco47I | GGWCC | 3 cut(s) | 300, 467, 3088 |
| Eco72I | CACGTG | 1 cut(s) | 3044 |
| Eco88I | CYCGRG | 4 cut(s) | 283, 1307, 3524, 4706 |
| EcoO109I | RGGNCCY | 2 cut(s) | 467, 6126 |
| EcoRII | CCWGG | 6 cut(s) | 469, 1352, 2250, 2602, 4201, 5178 |
| EcoRV | GATATC | 4 cut(s) | 1386, 1989, 2850, 2979 |
| EcoT14I | CCWWGG | 4 cut(s) | 523, 651, 4455, 5923 |
| EcoT22I | ATGCAT | 1 cut(s) | 5474 |
| EcoT38I | GRGCYC | 3 cut(s) | 188, 4011, 6130 |
| EgeI | GGCGCC | 1 cut(s) | 530 |
| EheI | GGCGCC | 1 cut(s) | 530 |
| ErhI | CCWWGG | 4 cut(s) | 523, 651, 4455, 5923 |
| FalI | AAGNNNNNCTT | 8 cut(s) | 130, 162, 516, 548, 3534, 3566, 5059, 5091 |
| FaqI | GGGAC | 6 cut(s) | 2248, 2455, 3878, 4618, 5197, 5992 |
| FauI | CCCGC | 2 cut(s) | 1822, 2206 |
| FauNDI | CATATG | 1 cut(s) | 2388 |
| FbaI | TGATCA | 4 cut(s) | 1513, 4138, 4669, 4885 |
| FblI | GTMKAC | 5 cut(s) | 860, 915, 1276, 3021, 4974 |
| FriOI | GRGCYC | 3 cut(s) | 188, 4011, 6130 |
| GlaI | GCGC | 2 cut(s) | 18, 530 |
| GsaI | CCCAGC | 7 cut(s) | 5652, 5682, 5712, 5742, 5772, 5951, 6013 |
| GsuI | CTGGAG | 4 cut(s) | 816, 2355, 2640, 3690 |
| HaeII | RGCGCY | 1 cut(s) | 532 |
| HaeIII | GGCC | 8 cut(s) | 596, 1569, 2591, 4963, 5575, 5974, 6051, 6128 |
| HapII | CCGG | 4 cut(s) | 242, 995, 3896, 3930 |
| HgaI | GACGC | 5 cut(s) | 676, 1751, 4134, 4620, 5282 |
| HhaI | GCGC | 2 cut(s) | 19, 531 |
| Hin1I | GRCGYC | 2 cut(s) | 529, 668 |
| Hin6I | GCGC | 2 cut(s) | 17, 529 |
| HinP1I | GCGC | 2 cut(s) | 17, 529 |
| HincII | GTYRAC | 8 cut(s) | 279, 861, 2129, 3022, 4342, 4838, 5601, 6027 |
| HindII | GTYRAC | 8 cut(s) | 279, 861, 2129, 3022, 4342, 4838, 5601, 6027 |
| HindIII | AAGCTT | 2 cut(s) | 358, 1640 |
| HpaII | CCGG | 4 cut(s) | 242, 995, 3896, 3930 |
| Hpy99I | CGWCG | 2 cut(s) | 124, 5349 |
| HpyCH4IV | ACGT | 7 cut(s) | 119, 571, 3043, 3125, 3813, 6151, 6333 |
| HpySE526I | ACGT | 7 cut(s) | 119, 571, 3043, 3125, 3813, 6151, 6333 |
| Hsp92I | GRCGYC | 2 cut(s) | 529, 668 |
| HspAI | GCGC | 2 cut(s) | 17, 529 |
| KasI | GGCGCC | 1 cut(s) | 528 |
| KpnI | GGTACC | 2 cut(s) | 4545, 5898 |
| Ksp22I | TGATCA | 4 cut(s) | 1513, 4138, 4669, 4885 |
| LguI | GCTCTTC | 2 cut(s) | 3480, 3999 |
| MabI | ACCWGGT | 1 cut(s) | 4201 |
| MaeII | ACGT | 7 cut(s) | 119, 571, 3043, 3125, 3813, 6151, 6333 |
| MfeI | CAATTG | 5 cut(s) | 69, 1578, 3067, 4728, 4919 |
| MflI | RGATCY | 7 cut(s) | 95, 782, 1201, 1462, 1493, 2881, 5476 |
| Mly113I | GGCGCC | 1 cut(s) | 529 |
| MmeI | TCCRAC | 6 cut(s) | 17, 102, 1450, 1593, 2969, 5986 |
| Mph1103I | ATGCAT | 1 cut(s) | 5474 |
| MroXI | GAANNNNTTC | 8 cut(s) | 703, 1569, 2085, 2177, 2907, 3296, 3427, 3862 |
| MslI | CAYNNNNRTG | 4 cut(s) | 1055, 2391, 2502, 4140 |
| MspA1I | CMGCKG | 6 cut(s) | 3538, 4379, 4496, 4910, 4943, 5539 |
| MspCI | CTTAAG | 3 cut(s) | 136, 3551, 5480 |
| MspI | CCGG | 4 cut(s) | 242, 995, 3896, 3930 |
| MspR9I | CCNGG | 7 cut(s) | 471, 1354, 2252, 2604, 3896, 4203, 5180 |
| MunI | CAATTG | 5 cut(s) | 69, 1578, 3067, 4728, 4919 |
| Mva1269I | GAATGC | 4 cut(s) | 1219, 1318, 4909, 5203 |
| MvaI | CCWGG | 6 cut(s) | 471, 1354, 2252, 2604, 4203, 5180 |
| NarI | GGCGCC | 1 cut(s) | 529 |
| NciI | CCSGG | 1 cut(s) | 3896 |
| NcoI | CCATGG | 1 cut(s) | 651 |
| NdeI | CATATG | 1 cut(s) | 2388 |
| NheI | GCTAGC | 2 cut(s) | 1631, 5618 |
| NmuCI | GTSAC | 6 cut(s) | 734, 1302, 1946, 2771, 3697, 3922 |
| NsiI | ATGCAT | 1 cut(s) | 5474 |
| NspI | RCATGY | 6 cut(s) | 1122, 2779, 4836, 5171, 5499, 6137 |
| NspV | TTCGAA | 2 cut(s) | 1590, 4244 |
| PaeI | GCATGC | 1 cut(s) | 5499 |
| PaeR7I | CTCGAG | 1 cut(s) | 3524 |
| PagI | TCATGA | 4 cut(s) | 2922, 4672, 4888, 5275 |
| PasI | CCCWGGG | 1 cut(s) | 5179 |
| PceI | AGGCCT | 3 cut(s) | 1569, 5575, 5974 |
| PciI | ACATGT | 3 cut(s) | 2775, 4832, 6133 |
| PciSI | GCTCTTC | 2 cut(s) | 3480, 3999 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1894 |
| PctI | GAATGC | 4 cut(s) | 1219, 1318, 4909, 5203 |
| PdmI | GAANNNNTTC | 8 cut(s) | 703, 1569, 2085, 2177, 2907, 3296, 3427, 3862 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 4426, 5539 |
| PfoI | TCCNGGA | 2 cut(s) | 2250, 3894 |
| PluTI | GGCGCC | 1 cut(s) | 532 |
| PmaCI | CACGTG | 1 cut(s) | 3044 |
| PmlI | CACGTG | 1 cut(s) | 3044 |
| Ppu21I | YACGTR | 1 cut(s) | 3044 |
| PpuMI | RGGWCCY | 1 cut(s) | 467 |
| PscI | ACATGT | 3 cut(s) | 2775, 4832, 6133 |
| PshAI | GACNNNNGTC | 2 cut(s) | 1300, 4473 |
| PshBI | ATTAAT | 1 cut(s) | 612 |
| PsiI | TTATAA | 1 cut(s) | 591 |
| Psp1406I | AACGTT | 3 cut(s) | 571, 3125, 3813 |
| Psp5II | RGGWCCY | 1 cut(s) | 467 |
| Psp6I | CCWGG | 6 cut(s) | 469, 1352, 2250, 2602, 4201, 5178 |
| PspCI | CACGTG | 1 cut(s) | 3044 |
| PspFI | CCCAGC | 7 cut(s) | 5648, 5678, 5708, 5738, 5768, 5947, 6009 |
| PspGI | CCWGG | 6 cut(s) | 469, 1352, 2250, 2602, 4201, 5178 |
| PspOMI | GGGCCC | 1 cut(s) | 6126 |
| PspPI | GGNCC | 6 cut(s) | 300, 467, 3088, 6050, 6126, 6127 |
| PspPPI | RGGWCCY | 1 cut(s) | 467 |
| PstI | CTGCAG | 9 cut(s) | 1081, 3160, 4288, 4942, 5508, 5617, 5628, 5688, 5748 |
| PstNI | CAGNNNCTG | 4 cut(s) | 738, 1340, 4499, 5539 |
| PsuI | RGATCY | 7 cut(s) | 95, 782, 1201, 1462, 1493, 2881, 5476 |
| PvuII | CAGCTG | 6 cut(s) | 3538, 4379, 4496, 4910, 4943, 5539 |
| RseI | CAYNNNNRTG | 4 cut(s) | 1055, 2391, 2502, 4140 |
| Rsr2I | CGGWCCG | 1 cut(s) | 3088 |
| RsrII | CGGWCCG | 1 cut(s) | 3088 |
| SalI | GTCGAC | 2 cut(s) | 859, 3020 |
| SapI | GCTCTTC | 2 cut(s) | 3480, 3999 |
| Sau96I | GGNCC | 6 cut(s) | 300, 467, 3088, 6050, 6126, 6127 |
| ScaI | AGTACT | 3 cut(s) | 196, 1367, 3769 |
| ScrFI | CCNGG | 7 cut(s) | 471, 1354, 2252, 2604, 3896, 4203, 5180 |
| SexAI | ACCWGGT | 1 cut(s) | 4201 |
| SfoI | GGCGCC | 1 cut(s) | 530 |
| Sfr274I | CTCGAG | 1 cut(s) | 3524 |
| SfuI | TTCGAA | 2 cut(s) | 1590, 4244 |
| SinI | GGWCC | 3 cut(s) | 300, 467, 3088 |
| SlaI | CTCGAG | 1 cut(s) | 3524 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 1055, 2391, 2502, 4140 |
| SmlI | CTYRAG | 5 cut(s) | 136, 215, 3524, 3551, 5480 |
| SmoI | CTYRAG | 5 cut(s) | 136, 215, 3524, 3551, 5480 |
| SphI | GCATGC | 1 cut(s) | 5499 |
| SseBI | AGGCCT | 3 cut(s) | 1569, 5575, 5974 |
| SsiI | CCGC | 9 cut(s) | 1635, 1815, 2199, 3086, 3109, 3136, 4280, 5049, 5499 |
| SspDI | GGCGCC | 1 cut(s) | 528 |
| SspI | AATATT | 2 cut(s) | 2110, 2456 |
| StuI | AGGCCT | 3 cut(s) | 1569, 5575, 5974 |
| StyD4I | CCNGG | 7 cut(s) | 469, 1352, 2250, 2602, 3894, 4201, 5178 |
| StyI | CCWWGG | 4 cut(s) | 523, 651, 4455, 5923 |
| TaiI | ACGT | 7 cut(s) | 122, 574, 3046, 3128, 3816, 6154, 6336 |
| TaqII | GACCGA | 1 cut(s) | 3730 |
| TatI | WGTACW | 8 cut(s) | 194, 1022, 1365, 2732, 3767, 4792, 4829, 4915 |
| TauI | GCSGC | 1 cut(s) | 1638 |
| TseFI | GTSAC | 6 cut(s) | 734, 1302, 1946, 2771, 3697, 3922 |
| Tsp45I | GTSAC | 6 cut(s) | 734, 1302, 1946, 2771, 3697, 3922 |
| TspGWI | ACGGA | 5 cut(s) | 1273, 2572, 4169, 5853, 5974 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 4426, 5539 |
| Vha464I | CTTAAG | 3 cut(s) | 136, 3551, 5480 |
| VneI | GTGCAC | 4 cut(s) | 2148, 3732, 5697, 5757 |
| VpaK11BI | GGWCC | 3 cut(s) | 300, 467, 3088 |
| VspI | ATTAAT | 1 cut(s) | 612 |
| XbaI | TCTAGA | 2 cut(s) | 149, 1198 |
| XceI | RCATGY | 6 cut(s) | 1122, 2779, 4836, 5171, 5499, 6137 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 2412 |
| XhoI | CTCGAG | 1 cut(s) | 3524 |
| XmiI | GTMKAC | 5 cut(s) | 860, 915, 1276, 3021, 4974 |
| XmnI | GAANNNNTTC | 8 cut(s) | 703, 1569, 2085, 2177, 2907, 3296, 3427, 3862 |
| ZrmI | AGTACT | 3 cut(s) | 196, 1367, 3769 |
| Zsp2I | ATGCAT | 1 cut(s) | 5474 |