helicase protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 591 |
| Acc36I | ACCTGC | 2 cut(s) | 70, 5791 |
| Acc65I | GGTACC | 2 cut(s) | 4475, 5888 |
| AccB1I | GGYRCC | 4 cut(s) | 528, 4475, 5888, 5987 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 4360, 5473 |
| AccI | GTMKAC | 5 cut(s) | 860, 915, 1276, 3021, 4908 |
| AccII | CGCG | 1 cut(s) | 2201 |
| AciI | CCGC | 7 cut(s) | 1635, 3086, 3109, 3136, 4214, 4983, 5433 |
| AclI | AACGTT | 3 cut(s) | 571, 3125, 3795 |
| AclWI | GGATC | 8 cut(s) | 90, 103, 790, 1196, 3716, 5144, 5418, 6263 |
| AcoI | YGGCCR | 2 cut(s) | 2589, 4895 |
| AcvI | CACGTG | 1 cut(s) | 3044 |
| AcyI | GRCGYC | 2 cut(s) | 529, 668 |
| AdeI | CACNNNGTG | 1 cut(s) | 3502 |
| AfiI | CCNNNNNNNGG | 9 cut(s) | 1001, 4360, 5039, 5322, 5473, 5885, 5921, 6116, 6117 |
| AflII | CTTAAG | 3 cut(s) | 136, 3551, 5414 |
| AflIII | ACRYGT | 3 cut(s) | 2775, 4766, 6127 |
| AjnI | CCWGG | 6 cut(s) | 469, 1352, 2250, 2602, 4135, 5112 |
| AjuI | GAANNNNNNNTTGG | 6 cut(s) | 1595, 1627, 4025, 4057, 5363, 5395 |
| Alw21I | GWGCWC | 7 cut(s) | 1477, 2152, 3718, 4347, 5635, 5695, 5755 |
| Alw26I | GTCTC | 8 cut(s) | 844, 1877, 3271, 3834, 4085, 4388, 5211, 5285 |
| Alw44I | GTGCAC | 5 cut(s) | 2148, 3714, 5631, 5691, 5751 |
| AlwI | GGATC | 8 cut(s) | 90, 103, 790, 1196, 3716, 5144, 5418, 6263 |
| AlwNI | CAGNNNCTG | 4 cut(s) | 738, 1340, 4433, 5473 |
| Ama87I | CYCGRG | 4 cut(s) | 283, 1307, 3524, 4640 |
| AoxI | GGCC | 8 cut(s) | 594, 1567, 2589, 4895, 5507, 5966, 6043, 6120 |
| ApaI | GGGCCC | 1 cut(s) | 6124 |
| ApaLI | GTGCAC | 5 cut(s) | 2148, 3714, 5631, 5691, 5751 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 4084, 4116 |
| AseI | ATTAAT | 1 cut(s) | 612 |
| Asp700I | GAANNNNTTC | 7 cut(s) | 703, 1569, 2085, 2177, 2907, 3296, 3427 |
| Asp718I | GGTACC | 2 cut(s) | 4475, 5888 |
| AspLEI | GCGC | 1 cut(s) | 531 |
| AspS9I | GGNCC | 7 cut(s) | 300, 467, 2509, 3088, 6044, 6120, 6121 |
| AsuC2I | CCSGG | 1 cut(s) | 3878 |
| AsuII | TTCGAA | 1 cut(s) | 4178 |
| AsuNHI | GCTAGC | 1 cut(s) | 5552 |
| AvaI | CYCGRG | 4 cut(s) | 283, 1307, 3524, 4640 |
| AvaII | GGWCC | 4 cut(s) | 300, 467, 2509, 3088 |
| BaeGI | GKGCMC | 7 cut(s) | 2152, 3718, 5635, 5695, 5755, 5941, 6124 |
| BaeI | ACNNNNGTAYC | 6 cut(s) | 43, 76, 117, 150, 5872, 5905 |
| BamHI | GGATCC | 1 cut(s) | 95 |
| BanI | GGYRCC | 4 cut(s) | 528, 4475, 5888, 5987 |
| BanII | GRGCYC | 3 cut(s) | 188, 3993, 6124 |
| BarI | GAAGNNNNNNTAC | 4 cut(s) | 733, 765, 1524, 1556 |
| BauI | CACGAG | 2 cut(s) | 501, 1887 |
| BbrPI | CACGTG | 1 cut(s) | 3044 |
| BbsI | GAAGAC | 4 cut(s) | 138, 1785, 3240, 3908 |
| Bbv12I | GWGCWC | 7 cut(s) | 1477, 2152, 3718, 4347, 5635, 5695, 5755 |
| BbvCI | CCTCAGC | 2 cut(s) | 5133, 5385 |
| BceAI | ACGGC | 1 cut(s) | 6058 |
| BciT130I | CCWGG | 6 cut(s) | 471, 1354, 2252, 2604, 4137, 5114 |
| BciVI | GTATCC | 2 cut(s) | 118, 4801 |
| BclI | TGATCA | 3 cut(s) | 1513, 4603, 4819 |
| BcnI | CCSGG | 1 cut(s) | 3878 |
| BcoDI | GTCTC | 8 cut(s) | 844, 1877, 3271, 3834, 4085, 4388, 5211, 5285 |
| BfoI | RGCGCY | 1 cut(s) | 532 |
| BfrI | CTTAAG | 3 cut(s) | 136, 3551, 5414 |
| BfuAI | ACCTGC | 2 cut(s) | 70, 5791 |
| BfuI | GTATCC | 2 cut(s) | 118, 4801 |
| BglII | AGATCT | 3 cut(s) | 1462, 1493, 2881 |
| BlpI | GCTNAGC | 1 cut(s) | 3048 |
| BmcAI | AGTACT | 3 cut(s) | 196, 1367, 3751 |
| Bme1390I | CCNGG | 7 cut(s) | 471, 1354, 2252, 2604, 3878, 4137, 5114 |
| Bme18I | GGWCC | 4 cut(s) | 300, 467, 2509, 3088 |
| BmeT110I | CYCGRG | 4 cut(s) | 283, 1307, 3524, 4640 |
| BmgT120I | GGNCC | 7 cut(s) | 300, 467, 2509, 3088, 6044, 6120, 6121 |
| BmrFI | CCNGG | 7 cut(s) | 471, 1354, 2252, 2604, 3878, 4137, 5114 |
| BmrI | ACTGGG | 6 cut(s) | 1378, 1952, 3762, 4278, 4761, 6296 |
| BmtI | GCTAGC | 1 cut(s) | 5556 |
| BmuI | ACTGGG | 6 cut(s) | 1378, 1952, 3762, 4278, 4761, 6296 |
| BoxI | GACNNNNGTC | 2 cut(s) | 1300, 4407 |
| BpiI | GAAGAC | 4 cut(s) | 138, 1785, 3240, 3908 |
| BpmI | CTGGAG | 6 cut(s) | 225, 816, 2355, 2640, 3672, 3895 |
| Bpu10I | CCTNAGC | 2 cut(s) | 5133, 5385 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 3048 |
| Bpu14I | TTCGAA | 1 cut(s) | 4178 |
| BpuEI | CTTGAG | 1 cut(s) | 236 |
| BpuMI | CCSGG | 1 cut(s) | 3878 |
| Bsa29I | ATCGAT | 1 cut(s) | 6217 |
| BsaAI | YACGTR | 1 cut(s) | 3044 |
| BsaHI | GRCGYC | 2 cut(s) | 529, 668 |
| BsaI | GGTCTC | 1 cut(s) | 4085 |
| BsaJI | CCNNGG | 8 cut(s) | 470, 523, 651, 4389, 4898, 5112, 5113, 5917 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 2634, 2664 |
| Bsc4I | CCNNNNNNNGG | 9 cut(s) | 1001, 4360, 5039, 5322, 5473, 5885, 5921, 6116, 6117 |
| Bse3DI | GCAATG | 4 cut(s) | 1614, 1839, 2182, 5291 |
| BseBI | CCWGG | 6 cut(s) | 471, 1354, 2252, 2604, 4137, 5114 |
| BseCI | ATCGAT | 1 cut(s) | 6217 |
| BseDI | CCNNGG | 8 cut(s) | 470, 523, 651, 4389, 4898, 5112, 5113, 5917 |
| BseLI | CCNNNNNNNGG | 9 cut(s) | 1001, 4360, 5039, 5322, 5473, 5885, 5921, 6116, 6117 |
| BseMI | GCAATG | 4 cut(s) | 1614, 1839, 2182, 5291 |
| BseRI | GAGGAG | 8 cut(s) | 103, 228, 2066, 2352, 3383, 4404, 5273, 6147 |
| BseSI | GKGCMC | 7 cut(s) | 2152, 3718, 5635, 5695, 5755, 5941, 6124 |
| BseYI | CCCAGC | 9 cut(s) | 5582, 5612, 5642, 5672, 5702, 5732, 5762, 5941, 6003 |
| BsgI | GTGCAG | 5 cut(s) | 805, 3459, 4980, 5459, 6159 |
| Bsh1236I | CGCG | 1 cut(s) | 2201 |
| BshFI | GGCC | 8 cut(s) | 596, 1569, 2591, 4897, 5509, 5968, 6045, 6122 |
| BshNI | GGYRCC | 4 cut(s) | 528, 4475, 5888, 5987 |
| BshVI | ATCGAT | 1 cut(s) | 6217 |
| BsiHKAI | GWGCWC | 7 cut(s) | 1477, 2152, 3718, 4347, 5635, 5695, 5755 |
| BsiHKCI | CYCGRG | 4 cut(s) | 283, 1307, 3524, 4640 |
| BsiSI | CCGG | 2 cut(s) | 995, 3878 |
| BslFI | GGGAC | 6 cut(s) | 2248, 2455, 3860, 4552, 5131, 5986 |
| BslI | CCNNNNNNNGG | 9 cut(s) | 1001, 4360, 5039, 5322, 5473, 5885, 5921, 6116, 6117 |
| BsmAI | GTCTC | 8 cut(s) | 844, 1877, 3271, 3834, 4085, 4388, 5211, 5285 |
| BsmFI | GGGAC | 6 cut(s) | 2248, 2455, 3860, 4552, 5131, 5986 |
| BsmI | GAATGC | 4 cut(s) | 1219, 1318, 4843, 5137 |
| BsnI | GGCC | 8 cut(s) | 596, 1569, 2591, 4897, 5509, 5968, 6045, 6122 |
| Bso31I | GGTCTC | 1 cut(s) | 4085 |
| BsoBI | CYCGRG | 4 cut(s) | 283, 1307, 3524, 4640 |
| Bsp119I | TTCGAA | 1 cut(s) | 4178 |
| Bsp120I | GGGCCC | 1 cut(s) | 6120 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 3048 |
| Bsp19I | CCATGG | 1 cut(s) | 651 |
| BspACI | CCGC | 7 cut(s) | 1635, 3086, 3109, 3136, 4214, 4983, 5433 |
| BspANI | GGCC | 8 cut(s) | 596, 1569, 2591, 4897, 5509, 5968, 6045, 6122 |
| BspDI | ATCGAT | 1 cut(s) | 6217 |
| BspFNI | CGCG | 1 cut(s) | 2201 |
| BspHI | TCATGA | 3 cut(s) | 2922, 4822, 5209 |
| BspMAI | CTGCAG | 9 cut(s) | 1081, 3160, 4222, 4876, 5551, 5562, 5622, 5682, 5742 |
| BspMI | ACCTGC | 2 cut(s) | 70, 5791 |
| BspOI | GCTAGC | 1 cut(s) | 5556 |
| BspPI | GGATC | 8 cut(s) | 90, 103, 790, 1196, 3716, 5144, 5418, 6263 |
| BspQI | GCTCTTC | 2 cut(s) | 3480, 3981 |
| BspT104I | TTCGAA | 1 cut(s) | 4178 |
| BspT107I | GGYRCC | 4 cut(s) | 528, 4475, 5888, 5987 |
| BspTI | CTTAAG | 3 cut(s) | 136, 3551, 5414 |
| BspTNI | GGTCTC | 1 cut(s) | 4085 |
| BsrDI | GCAATG | 4 cut(s) | 1614, 1839, 2182, 5291 |
| BssECI | CCNNGG | 8 cut(s) | 470, 523, 651, 4389, 4898, 5112, 5113, 5917 |
| BssNAI | GTATAC | 1 cut(s) | 1277 |
| BssNI | GRCGYC | 2 cut(s) | 529, 668 |
| BssSI | CACGAG | 2 cut(s) | 501, 1887 |
| BssT1I | CCWWGG | 4 cut(s) | 523, 651, 4898, 5917 |
| Bst1107I | GTATAC | 1 cut(s) | 1277 |
| Bst2BI | CACGAG | 2 cut(s) | 501, 1887 |
| Bst2UI | CCWGG | 6 cut(s) | 471, 1354, 2252, 2604, 4137, 5114 |
| Bst6I | CTCTTC | 7 cut(s) | 398, 600, 2683, 3075, 3480, 3981, 4605 |
| BstACI | GRCGYC | 2 cut(s) | 529, 668 |
| BstAFI | CTTAAG | 3 cut(s) | 136, 3551, 5414 |
| BstAPI | GCANNNNNTGC | 5 cut(s) | 3713, 3797, 4163, 5426, 6287 |
| BstBAI | YACGTR | 1 cut(s) | 3044 |
| BstBI | TTCGAA | 1 cut(s) | 4178 |
| BstDSI | CCRYGG | 1 cut(s) | 651 |
| BstFNI | CGCG | 1 cut(s) | 2201 |
| BstH2I | RGCGCY | 1 cut(s) | 532 |
| BstHHI | GCGC | 1 cut(s) | 531 |
| BstMAI | GTCTC | 8 cut(s) | 844, 1877, 3271, 3834, 4085, 4388, 5211, 5285 |
| BstNI | CCWGG | 6 cut(s) | 471, 1354, 2252, 2604, 4137, 5114 |
| BstNSI | RCATGY | 6 cut(s) | 1122, 2779, 4770, 5105, 5433, 6131 |
| BstPAI | GACNNNNGTC | 2 cut(s) | 1300, 4407 |
| BstSCI | CCNGG | 7 cut(s) | 469, 1352, 2250, 2602, 3876, 4135, 5112 |
| BstSLI | GKGCMC | 7 cut(s) | 2152, 3718, 5635, 5695, 5755, 5941, 6124 |
| BstUI | CGCG | 1 cut(s) | 2201 |
| BstV2I | GAAGAC | 4 cut(s) | 138, 1785, 3240, 3908 |
| BstX2I | RGATCY | 7 cut(s) | 95, 782, 1201, 1462, 1493, 2881, 5410 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 6011 |
| BstYI | RGATCY | 7 cut(s) | 95, 782, 1201, 1462, 1493, 2881, 5410 |
| BstZ17I | GTATAC | 1 cut(s) | 1277 |
| Bsu15I | ATCGAT | 1 cut(s) | 6217 |
| BsuI | GTATCC | 2 cut(s) | 118, 4801 |
| BsuRI | GGCC | 8 cut(s) | 596, 1569, 2591, 4897, 5509, 5968, 6045, 6122 |
| BsuTUI | ATCGAT | 1 cut(s) | 6217 |
| BtgI | CCRYGG | 1 cut(s) | 651 |
| BtsI | GCAGTG | 4 cut(s) | 739, 4164, 4940, 6276 |
| BveI | ACCTGC | 2 cut(s) | 70, 5791 |
| CaiI | CAGNNNCTG | 4 cut(s) | 738, 1340, 4433, 5473 |
| CciI | TCATGA | 3 cut(s) | 2922, 4822, 5209 |
| CfoI | GCGC | 1 cut(s) | 531 |
| Cfr13I | GGNCC | 7 cut(s) | 300, 467, 2509, 3088, 6044, 6120, 6121 |
| ClaI | ATCGAT | 1 cut(s) | 6217 |
| CpoI | CGGWCCG | 1 cut(s) | 3088 |
| CseI | GACGC | 6 cut(s) | 676, 1751, 2190, 4554, 5216, 6156 |
| CsiI | ACCWGGT | 1 cut(s) | 4135 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 4065, 4100 |
| CspI | CGGWCCG | 1 cut(s) | 3088 |
| DinI | GGCGCC | 1 cut(s) | 530 |
| DraIII | CACNNNGTG | 1 cut(s) | 3502 |
| EaeI | YGGCCR | 2 cut(s) | 2589, 4895 |
| Eam1104I | CTCTTC | 7 cut(s) | 398, 600, 2683, 3075, 3480, 3981, 4605 |
| EarI | CTCTTC | 7 cut(s) | 398, 600, 2683, 3075, 3480, 3981, 4605 |
| EciI | GGCGGA | 2 cut(s) | 3101, 4203 |
| Eco130I | CCWWGG | 4 cut(s) | 523, 651, 4898, 5917 |
| Eco147I | AGGCCT | 3 cut(s) | 1569, 5509, 5968 |
| Eco24I | GRGCYC | 3 cut(s) | 188, 3993, 6124 |
| Eco31I | GGTCTC | 1 cut(s) | 4085 |
| Eco32I | GATATC | 4 cut(s) | 1386, 1989, 2850, 2979 |
| Eco47I | GGWCC | 4 cut(s) | 300, 467, 2509, 3088 |
| Eco72I | CACGTG | 1 cut(s) | 3044 |
| Eco88I | CYCGRG | 4 cut(s) | 283, 1307, 3524, 4640 |
| EcoO109I | RGGNCCY | 3 cut(s) | 467, 2509, 6120 |
| EcoRII | CCWGG | 6 cut(s) | 469, 1352, 2250, 2602, 4135, 5112 |
| EcoRV | GATATC | 4 cut(s) | 1386, 1989, 2850, 2979 |
| EcoT14I | CCWWGG | 4 cut(s) | 523, 651, 4898, 5917 |
| EcoT22I | ATGCAT | 1 cut(s) | 5408 |
| EcoT38I | GRGCYC | 3 cut(s) | 188, 3993, 6124 |
| EgeI | GGCGCC | 1 cut(s) | 530 |
| EheI | GGCGCC | 1 cut(s) | 530 |
| ErhI | CCWWGG | 4 cut(s) | 523, 651, 4898, 5917 |
| FalI | AAGNNNNNCTT | 8 cut(s) | 130, 162, 516, 548, 3534, 3566, 4993, 5025 |
| FaqI | GGGAC | 6 cut(s) | 2248, 2455, 3860, 4552, 5131, 5986 |
| FauNDI | CATATG | 2 cut(s) | 2388, 6072 |
| FbaI | TGATCA | 3 cut(s) | 1513, 4603, 4819 |
| FblI | GTMKAC | 5 cut(s) | 860, 915, 1276, 3021, 4908 |
| FriOI | GRGCYC | 3 cut(s) | 188, 3993, 6124 |
| GlaI | GCGC | 1 cut(s) | 530 |
| GsaI | CCCAGC | 9 cut(s) | 5586, 5616, 5646, 5676, 5706, 5736, 5766, 5945, 6007 |
| GsuI | CTGGAG | 6 cut(s) | 225, 816, 2355, 2640, 3672, 3895 |
| HaeII | RGCGCY | 1 cut(s) | 532 |
| HaeIII | GGCC | 8 cut(s) | 596, 1569, 2591, 4897, 5509, 5968, 6045, 6122 |
| HapII | CCGG | 2 cut(s) | 995, 3878 |
| HgaI | GACGC | 6 cut(s) | 676, 1751, 2190, 4554, 5216, 6156 |
| HhaI | GCGC | 1 cut(s) | 531 |
| Hin1I | GRCGYC | 2 cut(s) | 529, 668 |
| Hin6I | GCGC | 1 cut(s) | 529 |
| HinP1I | GCGC | 1 cut(s) | 529 |
| HincII | GTYRAC | 8 cut(s) | 279, 861, 2129, 3022, 4276, 4772, 5535, 6021 |
| HindII | GTYRAC | 8 cut(s) | 279, 861, 2129, 3022, 4276, 4772, 5535, 6021 |
| HindIII | AAGCTT | 3 cut(s) | 358, 1629, 1640 |
| HpaII | CCGG | 2 cut(s) | 995, 3878 |
| Hpy99I | CGWCG | 2 cut(s) | 694, 5283 |
| HpyCH4IV | ACGT | 7 cut(s) | 571, 689, 3043, 3125, 3795, 6145, 6327 |
| HpySE526I | ACGT | 7 cut(s) | 571, 689, 3043, 3125, 3795, 6145, 6327 |
| Hsp92I | GRCGYC | 2 cut(s) | 529, 668 |
| HspAI | GCGC | 1 cut(s) | 529 |
| KasI | GGCGCC | 1 cut(s) | 528 |
| KpnI | GGTACC | 2 cut(s) | 4479, 5892 |
| Ksp22I | TGATCA | 3 cut(s) | 1513, 4603, 4819 |
| LguI | GCTCTTC | 2 cut(s) | 3480, 3981 |
| MabI | ACCWGGT | 1 cut(s) | 4135 |
| MaeII | ACGT | 7 cut(s) | 571, 689, 3043, 3125, 3795, 6145, 6327 |
| MfeI | CAATTG | 5 cut(s) | 69, 1578, 3067, 4662, 4853 |
| MflI | RGATCY | 7 cut(s) | 95, 782, 1201, 1462, 1493, 2881, 5410 |
| Mly113I | GGCGCC | 1 cut(s) | 529 |
| MmeI | TCCRAC | 7 cut(s) | 17, 1450, 1593, 2969, 5630, 5690, 5980 |
| Mph1103I | ATGCAT | 1 cut(s) | 5408 |
| MroXI | GAANNNNTTC | 7 cut(s) | 703, 1569, 2085, 2177, 2907, 3296, 3427 |
| MslI | CAYNNNNRTG | 4 cut(s) | 1055, 2391, 2502, 4122 |
| MspA1I | CMGCKG | 6 cut(s) | 3538, 4313, 4430, 4844, 4877, 5473 |
| MspCI | CTTAAG | 3 cut(s) | 136, 3551, 5414 |
| MspI | CCGG | 2 cut(s) | 995, 3878 |
| MspR9I | CCNGG | 7 cut(s) | 471, 1354, 2252, 2604, 3878, 4137, 5114 |
| MunI | CAATTG | 5 cut(s) | 69, 1578, 3067, 4662, 4853 |
| Mva1269I | GAATGC | 4 cut(s) | 1219, 1318, 4843, 5137 |
| MvaI | CCWGG | 6 cut(s) | 471, 1354, 2252, 2604, 4137, 5114 |
| MvnI | CGCG | 1 cut(s) | 2201 |
| NarI | GGCGCC | 1 cut(s) | 529 |
| NciI | CCSGG | 1 cut(s) | 3878 |
| NcoI | CCATGG | 1 cut(s) | 651 |
| NdeI | CATATG | 2 cut(s) | 2388, 6072 |
| NheI | GCTAGC | 1 cut(s) | 5552 |
| NmuCI | GTSAC | 6 cut(s) | 734, 1302, 1946, 2771, 3679, 3904 |
| NsiI | ATGCAT | 1 cut(s) | 5408 |
| NspI | RCATGY | 6 cut(s) | 1122, 2779, 4770, 5105, 5433, 6131 |
| NspV | TTCGAA | 1 cut(s) | 4178 |
| PaeI | GCATGC | 1 cut(s) | 5433 |
| PaeR7I | CTCGAG | 1 cut(s) | 3524 |
| PagI | TCATGA | 3 cut(s) | 2922, 4822, 5209 |
| PasI | CCCWGGG | 1 cut(s) | 5113 |
| PceI | AGGCCT | 3 cut(s) | 1569, 5509, 5968 |
| PciI | ACATGT | 3 cut(s) | 2775, 4766, 6127 |
| PciSI | GCTCTTC | 2 cut(s) | 3480, 3981 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1894 |
| PctI | GAATGC | 4 cut(s) | 1219, 1318, 4843, 5137 |
| PdmI | GAANNNNTTC | 7 cut(s) | 703, 1569, 2085, 2177, 2907, 3296, 3427 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 4360, 5473 |
| PfoI | TCCNGGA | 2 cut(s) | 2250, 3876 |
| PluTI | GGCGCC | 1 cut(s) | 532 |
| PmaCI | CACGTG | 1 cut(s) | 3044 |
| PmlI | CACGTG | 1 cut(s) | 3044 |
| Ppu21I | YACGTR | 1 cut(s) | 3044 |
| PpuMI | RGGWCCY | 2 cut(s) | 467, 2509 |
| PscI | ACATGT | 3 cut(s) | 2775, 4766, 6127 |
| PshAI | GACNNNNGTC | 2 cut(s) | 1300, 4407 |
| PshBI | ATTAAT | 1 cut(s) | 612 |
| PsiI | TTATAA | 1 cut(s) | 591 |
| Psp1406I | AACGTT | 3 cut(s) | 571, 3125, 3795 |
| Psp5II | RGGWCCY | 2 cut(s) | 467, 2509 |
| Psp6I | CCWGG | 6 cut(s) | 469, 1352, 2250, 2602, 4135, 5112 |
| PspCI | CACGTG | 1 cut(s) | 3044 |
| PspFI | CCCAGC | 9 cut(s) | 5582, 5612, 5642, 5672, 5702, 5732, 5762, 5941, 6003 |
| PspGI | CCWGG | 6 cut(s) | 469, 1352, 2250, 2602, 4135, 5112 |
| PspOMI | GGGCCC | 1 cut(s) | 6120 |
| PspPI | GGNCC | 7 cut(s) | 300, 467, 2509, 3088, 6044, 6120, 6121 |
| PspPPI | RGGWCCY | 2 cut(s) | 467, 2509 |
| PstI | CTGCAG | 9 cut(s) | 1081, 3160, 4222, 4876, 5551, 5562, 5622, 5682, 5742 |
| PstNI | CAGNNNCTG | 4 cut(s) | 738, 1340, 4433, 5473 |
| PsuI | RGATCY | 7 cut(s) | 95, 782, 1201, 1462, 1493, 2881, 5410 |
| PvuII | CAGCTG | 6 cut(s) | 3538, 4313, 4430, 4844, 4877, 5473 |
| RseI | CAYNNNNRTG | 4 cut(s) | 1055, 2391, 2502, 4122 |
| Rsr2I | CGGWCCG | 1 cut(s) | 3088 |
| RsrII | CGGWCCG | 1 cut(s) | 3088 |
| SalI | GTCGAC | 2 cut(s) | 859, 3020 |
| SapI | GCTCTTC | 2 cut(s) | 3480, 3981 |
| Sau96I | GGNCC | 7 cut(s) | 300, 467, 2509, 3088, 6044, 6120, 6121 |
| ScaI | AGTACT | 3 cut(s) | 196, 1367, 3751 |
| ScrFI | CCNGG | 7 cut(s) | 471, 1354, 2252, 2604, 3878, 4137, 5114 |
| SexAI | ACCWGGT | 1 cut(s) | 4135 |
| SfoI | GGCGCC | 1 cut(s) | 530 |
| Sfr274I | CTCGAG | 1 cut(s) | 3524 |
| SfuI | TTCGAA | 1 cut(s) | 4178 |
| SinI | GGWCC | 4 cut(s) | 300, 467, 2509, 3088 |
| SlaI | CTCGAG | 1 cut(s) | 3524 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 1055, 2391, 2502, 4122 |
| SmlI | CTYRAG | 5 cut(s) | 136, 215, 3524, 3551, 5414 |
| SmoI | CTYRAG | 5 cut(s) | 136, 215, 3524, 3551, 5414 |
| SphI | GCATGC | 1 cut(s) | 5433 |
| SseBI | AGGCCT | 3 cut(s) | 1569, 5509, 5968 |
| SsiI | CCGC | 7 cut(s) | 1635, 3086, 3109, 3136, 4214, 4983, 5433 |
| SspDI | GGCGCC | 1 cut(s) | 528 |
| SspI | AATATT | 2 cut(s) | 2110, 2456 |
| StuI | AGGCCT | 3 cut(s) | 1569, 5509, 5968 |
| StyD4I | CCNGG | 7 cut(s) | 469, 1352, 2250, 2602, 3876, 4135, 5112 |
| StyI | CCWWGG | 4 cut(s) | 523, 651, 4898, 5917 |
| TaiI | ACGT | 7 cut(s) | 574, 692, 3046, 3128, 3798, 6148, 6330 |
| TatI | WGTACW | 8 cut(s) | 194, 1022, 1365, 2732, 3749, 4726, 4849, 6061 |
| TauI | GCSGC | 1 cut(s) | 1638 |
| TseFI | GTSAC | 6 cut(s) | 734, 1302, 1946, 2771, 3679, 3904 |
| Tsp45I | GTSAC | 6 cut(s) | 734, 1302, 1946, 2771, 3679, 3904 |
| TspGWI | ACGGA | 4 cut(s) | 1273, 2572, 5847, 5968 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 4360, 5473 |
| Vha464I | CTTAAG | 3 cut(s) | 136, 3551, 5414 |
| VneI | GTGCAC | 5 cut(s) | 2148, 3714, 5631, 5691, 5751 |
| VpaK11BI | GGWCC | 4 cut(s) | 300, 467, 2509, 3088 |
| VspI | ATTAAT | 1 cut(s) | 612 |
| XbaI | TCTAGA | 2 cut(s) | 149, 1198 |
| XceI | RCATGY | 6 cut(s) | 1122, 2779, 4770, 5105, 5433, 6131 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 2412 |
| XhoI | CTCGAG | 1 cut(s) | 3524 |
| XmiI | GTMKAC | 5 cut(s) | 860, 915, 1276, 3021, 4908 |
| XmnI | GAANNNNTTC | 7 cut(s) | 703, 1569, 2085, 2177, 2907, 3296, 3427 |
| ZrmI | AGTACT | 3 cut(s) | 196, 1367, 3751 |
| Zsp2I | ATGCAT | 1 cut(s) | 5408 |