helicase protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1700 |
| AarI | CACCTGC | 1 cut(s) | 631 |
| Acc36I | ACCTGC | 1 cut(s) | 631 |
| Acc65I | GGTACC | 1 cut(s) | 1411 |
| AccB1I | GGYRCC | 3 cut(s) | 1411, 1716, 2026 |
| AciI | CCGC | 2 cut(s) | 369, 891 |
| AclWI | GGATC | 4 cut(s) | 61, 433, 920, 1196 |
| AcsI | RAATTY | 7 cut(s) | 183, 286, 446, 558, 1091, 1786, 2518 |
| AdeI | CACNNNGTG | 1 cut(s) | 1485 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 652, 1013, 1726, 2265, 2460 |
| AflII | CTTAAG | 1 cut(s) | 2291 |
| AflIII | ACRYGT | 1 cut(s) | 634 |
| AjnI | CCWGG | 4 cut(s) | 646, 1473, 2274, 2453 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1548, 1580 |
| AleI | CACNNNNGTG | 1 cut(s) | 1875 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1997, 2029 |
| Alw21I | GWGCWC | 1 cut(s) | 725 |
| Alw26I | GTCTC | 3 cut(s) | 847, 1545, 2333 |
| AlwI | GGATC | 4 cut(s) | 61, 433, 920, 1196 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 995 |
| AoxI | GGCC | 3 cut(s) | 506, 1693, 1961 |
| ApeKI | GCWGC | 6 cut(s) | 513, 954, 1069, 1516, 1549, 1664 |
| ApoI | RAATTY | 7 cut(s) | 183, 286, 446, 558, 1091, 1786, 2518 |
| AseI | ATTAAT | 1 cut(s) | 1535 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 2012 |
| Asp718I | GGTACC | 1 cut(s) | 1411 |
| AspS9I | GGNCC | 3 cut(s) | 683, 861, 1693 |
| AsuHPI | GGTGA | 9 cut(s) | 662, 1118, 1592, 1671, 1741, 1889, 1933, 2127, 2372 |
| AsuII | TTCGAA | 1 cut(s) | 2169 |
| AsuNHI | GCTAGC | 1 cut(s) | 1801 |
| AvaII | GGWCC | 2 cut(s) | 683, 861 |
| AxyI | CCTNAGG | 1 cut(s) | 1683 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 623, 656 |
| BanI | GGYRCC | 3 cut(s) | 1411, 1716, 2026 |
| BanII | GRGCYC | 2 cut(s) | 146, 725 |
| Bbv12I | GWGCWC | 1 cut(s) | 725 |
| BbvI | GCAGC | 6 cut(s) | 500, 966, 1081, 1503, 1561, 1651 |
| BccI | CCATC | 7 cut(s) | 389, 542, 862, 1194, 1337, 2038, 2175 |
| BciT130I | CCWGG | 4 cut(s) | 648, 1475, 2276, 2455 |
| BciVI | GTATCC | 1 cut(s) | 76 |
| BclI | TGATCA | 2 cut(s) | 1971, 2362 |
| BcoDI | GTCTC | 3 cut(s) | 847, 1545, 2333 |
| BfaI | CTAG | 8 cut(s) | 108, 156, 510, 1199, 1317, 1802, 1838, 2501 |
| BfmI | CTRYAG | 3 cut(s) | 687, 795, 1086 |
| BfrI | CTTAAG | 1 cut(s) | 2291 |
| BfuAI | ACCTGC | 1 cut(s) | 631 |
| BfuI | GTATCC | 1 cut(s) | 76 |
| BisI | GCNGC | 7 cut(s) | 370, 514, 955, 1070, 1517, 1550, 1665 |
| BlsI | GCNGC | 7 cut(s) | 371, 515, 956, 1071, 1518, 1551, 1666 |
| BmcAI | AGTACT | 4 cut(s) | 61, 2124, 2289, 2510 |
| Bme1390I | CCNGG | 4 cut(s) | 648, 1475, 2276, 2455 |
| Bme18I | GGWCC | 2 cut(s) | 683, 861 |
| BmgT120I | GGNCC | 3 cut(s) | 683, 861, 1693 |
| BmiI | GGNNCC | 5 cut(s) | 862, 1413, 1718, 2028, 2475 |
| BmrFI | CCNGG | 4 cut(s) | 648, 1475, 2276, 2455 |
| BmtI | GCTAGC | 1 cut(s) | 1805 |
| BplI | GAGNNNNNCTC | 6 cut(s) | 302, 334, 932, 964, 1005, 1037 |
| BpmI | CTGGAG | 3 cut(s) | 93, 959, 1827 |
| Bpu10I | CCTNAGC | 3 cut(s) | 319, 1545, 2372 |
| Bpu14I | TTCGAA | 1 cut(s) | 2169 |
| BpuEI | CTTGAG | 1 cut(s) | 481 |
| BsaAI | YACGTR | 3 cut(s) | 635, 1859, 2255 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1956 |
| BsaJI | CCNNGG | 4 cut(s) | 251, 503, 646, 2453 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 229, 259, 1997, 2027 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 652, 1013, 1726, 2265, 2460 |
| Bse118I | RCCGGY | 1 cut(s) | 1719 |
| Bse1I | ACTGG | 2 cut(s) | 582, 2362 |
| Bse21I | CCTNAGG | 1 cut(s) | 1683 |
| Bse3DI | GCAATG | 3 cut(s) | 379, 848, 1461 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1956 |
| BseBI | CCWGG | 4 cut(s) | 648, 1475, 2276, 2455 |
| BseDI | CCNNGG | 4 cut(s) | 251, 503, 646, 2453 |
| BseJI | GATNNNNATC | 1 cut(s) | 1956 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 652, 1013, 1726, 2265, 2460 |
| BseMI | GCAATG | 3 cut(s) | 379, 848, 1461 |
| BseMII | CTCAG | 6 cut(s) | 333, 351, 522, 987, 1674, 2298 |
| BseNI | ACTGG | 2 cut(s) | 582, 2362 |
| BseRI | GAGGAG | 5 cut(s) | 186, 347, 835, 1029, 1376 |
| BseXI | GCAGC | 6 cut(s) | 500, 966, 1081, 1503, 1561, 1651 |
| BsgI | GTGCAG | 2 cut(s) | 805, 2258 |
| Bsh1285I | CGRYCG | 1 cut(s) | 2566 |
| BshFI | GGCC | 3 cut(s) | 508, 1695, 1963 |
| BshNI | GGYRCC | 3 cut(s) | 1411, 1716, 2026 |
| BsiEI | CGRYCG | 1 cut(s) | 2566 |
| BsiHKAI | GWGCWC | 1 cut(s) | 725 |
| BsiSI | CCGG | 1 cut(s) | 1720 |
| BslFI | GGGAC | 2 cut(s) | 475, 1227 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 652, 1013, 1726, 2265, 2460 |
| BsmAI | GTCTC | 3 cut(s) | 847, 1545, 2333 |
| BsmFI | GGGAC | 2 cut(s) | 475, 1227 |
| BsmI | GAATGC | 2 cut(s) | 1666, 1888 |
| BsnI | GGCC | 3 cut(s) | 508, 1695, 1963 |
| Bsp119I | TTCGAA | 1 cut(s) | 2169 |
| Bsp1286I | GDGCHC | 2 cut(s) | 146, 725 |
| Bsp1407I | TGTACA | 1 cut(s) | 2479 |
| BspACI | CCGC | 2 cut(s) | 369, 891 |
| BspANI | GGCC | 3 cut(s) | 508, 1695, 1963 |
| BspCNI | CTCAG | 6 cut(s) | 332, 352, 523, 988, 1675, 2299 |
| BspLI | GGNNCC | 5 cut(s) | 862, 1413, 1718, 2028, 2475 |
| BspMAI | CTGCAG | 1 cut(s) | 799 |
| BspMI | ACCTGC | 1 cut(s) | 631 |
| BspOI | GCTAGC | 1 cut(s) | 1805 |
| BspPI | GGATC | 4 cut(s) | 61, 433, 920, 1196 |
| BspT104I | TTCGAA | 1 cut(s) | 2169 |
| BspT107I | GGYRCC | 3 cut(s) | 1411, 1716, 2026 |
| BspTI | CTTAAG | 1 cut(s) | 2291 |
| BsrDI | GCAATG | 3 cut(s) | 379, 848, 1461 |
| BsrFI | RCCGGY | 1 cut(s) | 1719 |
| BsrGI | TGTACA | 1 cut(s) | 2479 |
| BsrI | ACTGG | 2 cut(s) | 582, 2362 |
| BssAI | RCCGGY | 1 cut(s) | 1719 |
| BssECI | CCNNGG | 4 cut(s) | 251, 503, 646, 2453 |
| BssT1I | CCWWGG | 1 cut(s) | 503 |
| Bst2UI | CCWGG | 4 cut(s) | 648, 1475, 2276, 2455 |
| Bst6I | CTCTTC | 1 cut(s) | 1159 |
| BstAFI | CTTAAG | 1 cut(s) | 2291 |
| BstAUI | TGTACA | 1 cut(s) | 2479 |
| BstBAI | YACGTR | 3 cut(s) | 635, 1859, 2255 |
| BstBI | TTCGAA | 1 cut(s) | 2169 |
| BstC8I | GCNNGC | 5 cut(s) | 344, 1067, 1721, 1803, 2206 |
| BstDEI | CTNAG | 9 cut(s) | 319, 360, 531, 553, 996, 1545, 1683, 2307, 2372 |
| BstEII | GGTNACC | 1 cut(s) | 1106 |
| BstMAI | GTCTC | 3 cut(s) | 847, 1545, 2333 |
| BstMCI | CGRYCG | 1 cut(s) | 2566 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 343, 1034, 1179, 1861 |
| BstNI | CCWGG | 4 cut(s) | 648, 1475, 2276, 2455 |
| BstNSI | RCATGY | 3 cut(s) | 900, 1069, 1122 |
| BstPI | GGTNACC | 1 cut(s) | 1106 |
| BstSCI | CCNGG | 4 cut(s) | 646, 1473, 2274, 2453 |
| BstSFI | CTRYAG | 3 cut(s) | 687, 795, 1086 |
| BstSNI | TACGTA | 1 cut(s) | 2255 |
| BstV1I | GCAGC | 6 cut(s) | 500, 966, 1081, 1503, 1561, 1651 |
| BstX2I | RGATCY | 2 cut(s) | 425, 1201 |
| BstYI | RGATCY | 2 cut(s) | 425, 1201 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1683 |
| BsuI | GTATCC | 1 cut(s) | 76 |
| BsuRI | GGCC | 3 cut(s) | 508, 1695, 1963 |
| BtgZI | GCGATG | 1 cut(s) | 561 |
| BtsI | GCAGTG | 3 cut(s) | 426, 2308, 2389 |
| BveI | ACCTGC | 1 cut(s) | 631 |
| Cac8I | GCNNGC | 5 cut(s) | 344, 1067, 1721, 1803, 2206 |
| CaiI | CAGNNNCTG | 1 cut(s) | 995 |
| Cfr10I | RCCGGY | 1 cut(s) | 1719 |
| Cfr13I | GGNCC | 3 cut(s) | 683, 861, 1693 |
| CsiI | ACCWGGT | 1 cut(s) | 1473 |
| CviAII | CATG | 9 cut(s) | 517, 598, 718, 897, 1066, 1119, 1246, 1833, 2432 |
| DdeI | CTNAG | 9 cut(s) | 319, 360, 531, 553, 996, 1545, 1683, 2307, 2372 |
| DraIII | CACNNNGTG | 1 cut(s) | 1485 |
| Eam1104I | CTCTTC | 1 cut(s) | 1159 |
| EarI | CTCTTC | 1 cut(s) | 1159 |
| Ecl136II | GAGCTC | 1 cut(s) | 723 |
| Eco105I | TACGTA | 1 cut(s) | 2255 |
| Eco130I | CCWWGG | 1 cut(s) | 503 |
| Eco147I | AGGCCT | 1 cut(s) | 508 |
| Eco24I | GRGCYC | 2 cut(s) | 146, 725 |
| Eco32I | GATATC | 1 cut(s) | 2487 |
| Eco47I | GGWCC | 2 cut(s) | 683, 861 |
| Eco53kI | GAGCTC | 1 cut(s) | 723 |
| Eco81I | CCTNAGG | 1 cut(s) | 1683 |
| Eco91I | GGTNACC | 1 cut(s) | 1106 |
| EcoICRI | GAGCTC | 1 cut(s) | 723 |
| EcoO109I | RGGNCCY | 2 cut(s) | 683, 1693 |
| EcoO65I | GGTNACC | 1 cut(s) | 1106 |
| EcoRII | CCWGG | 4 cut(s) | 646, 1473, 2274, 2453 |
| EcoRV | GATATC | 1 cut(s) | 2487 |
| EcoT14I | CCWWGG | 1 cut(s) | 503 |
| EcoT38I | GRGCYC | 2 cut(s) | 146, 725 |
| ErhI | CCWWGG | 1 cut(s) | 503 |
| FaeI | CATG | 9 cut(s) | 520, 601, 721, 900, 1069, 1122, 1249, 1836, 2435 |
| FaqI | GGGAC | 2 cut(s) | 475, 1227 |
| FatI | CATG | 9 cut(s) | 516, 597, 717, 896, 1065, 1118, 1245, 1832, 2431 |
| FauNDI | CATATG | 1 cut(s) | 1042 |
| FbaI | TGATCA | 2 cut(s) | 1971, 2362 |
| Fnu4HI | GCNGC | 7 cut(s) | 370, 514, 955, 1070, 1517, 1550, 1665 |
| FriOI | GRGCYC | 2 cut(s) | 146, 725 |
| Fsp4HI | GCNGC | 7 cut(s) | 370, 514, 955, 1070, 1517, 1550, 1665 |
| FspBI | CTAG | 8 cut(s) | 108, 156, 510, 1199, 1317, 1802, 1838, 2501 |
| GluI | GCNGC | 7 cut(s) | 370, 514, 955, 1070, 1517, 1550, 1665 |
| GsuI | CTGGAG | 3 cut(s) | 93, 959, 1827 |
| HaeIII | GGCC | 3 cut(s) | 508, 1695, 1963 |
| HapII | CCGG | 1 cut(s) | 1720 |
| Hin1II | CATG | 9 cut(s) | 520, 601, 721, 900, 1069, 1122, 1249, 1836, 2435 |
| HincII | GTYRAC | 3 cut(s) | 237, 569, 2640 |
| HindII | GTYRAC | 3 cut(s) | 237, 569, 2640 |
| HindIII | AAGCTT | 2 cut(s) | 525, 1055 |
| HpaII | CCGG | 1 cut(s) | 1720 |
| HphI | GGTGA | 9 cut(s) | 662, 1118, 1592, 1671, 1741, 1889, 1933, 2127, 2372 |
| Hpy166II | GTNNAC | 6 cut(s) | 237, 569, 864, 1500, 2050, 2640 |
| Hpy8I | GTNNAC | 6 cut(s) | 237, 569, 864, 1500, 2050, 2640 |
| HpyAV | CCTTC | 4 cut(s) | 156, 766, 1912, 1995 |
| HpyCH4IV | ACGT | 4 cut(s) | 634, 768, 1858, 2254 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 343, 1034, 1179, 1861 |
| HpyF3I | CTNAG | 9 cut(s) | 319, 360, 531, 553, 996, 1545, 1683, 2307, 2372 |
| HpySE526I | ACGT | 4 cut(s) | 634, 768, 1858, 2254 |
| Hsp92II | CATG | 9 cut(s) | 520, 601, 721, 900, 1069, 1122, 1249, 1836, 2435 |
| KpnI | GGTACC | 1 cut(s) | 1415 |
| KroI | GCCGGC | 1 cut(s) | 1719 |
| KroNI | GCCGGC | 1 cut(s) | 1721 |
| Ksp22I | TGATCA | 2 cut(s) | 1971, 2362 |
| LmnI | GCTCC | 4 cut(s) | 74, 334, 1444, 2573 |
| Lsp1109I | GCAGC | 6 cut(s) | 500, 966, 1081, 1503, 1561, 1651 |
| MabI | ACCWGGT | 1 cut(s) | 1473 |
| MaeI | CTAG | 8 cut(s) | 108, 156, 510, 1199, 1317, 1802, 1838, 2501 |
| MaeII | ACGT | 4 cut(s) | 634, 768, 1858, 2254 |
| MaeIII | GTNAC | 4 cut(s) | 742, 1106, 1477, 2128 |
| MfeI | CAATTG | 1 cut(s) | 659 |
| MflI | RGATCY | 2 cut(s) | 425, 1201 |
| MhlI | GDGCHC | 2 cut(s) | 146, 725 |
| MlyI | GAGTC | 5 cut(s) | 165, 232, 276, 1310, 2441 |
| MmeI | TCCRAC | 3 cut(s) | 266, 862, 2551 |
| MroNI | GCCGGC | 1 cut(s) | 1719 |
| MroXI | GAANNNNTTC | 1 cut(s) | 2012 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1578, 1875 |
| MspA1I | CMGCKG | 1 cut(s) | 1516 |
| MspCI | CTTAAG | 1 cut(s) | 2291 |
| MspI | CCGG | 1 cut(s) | 1720 |
| MspR9I | CCNGG | 4 cut(s) | 648, 1475, 2276, 2455 |
| MunI | CAATTG | 1 cut(s) | 659 |
| Mva1269I | GAATGC | 2 cut(s) | 1666, 1888 |
| MvaI | CCWGG | 4 cut(s) | 648, 1475, 2276, 2455 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 343, 1034, 1179, 1861 |
| NaeI | GCCGGC | 1 cut(s) | 1721 |
| NdeI | CATATG | 1 cut(s) | 1042 |
| NgoMIV | GCCGGC | 1 cut(s) | 1719 |
| NheI | GCTAGC | 1 cut(s) | 1801 |
| NlaIII | CATG | 9 cut(s) | 520, 601, 721, 900, 1069, 1122, 1249, 1836, 2435 |
| NlaIV | GGNNCC | 5 cut(s) | 862, 1413, 1718, 2028, 2475 |
| NmuCI | GTSAC | 2 cut(s) | 1106, 1477 |
| NspI | RCATGY | 3 cut(s) | 900, 1069, 1122 |
| NspV | TTCGAA | 1 cut(s) | 2169 |
| OliI | CACNNNNGTG | 1 cut(s) | 1875 |
| PaeI | GCATGC | 1 cut(s) | 1069 |
| PaqCI | CACCTGC | 1 cut(s) | 631 |
| PceI | AGGCCT | 1 cut(s) | 508 |
| PctI | GAATGC | 2 cut(s) | 1666, 1888 |
| PdiI | GCCGGC | 1 cut(s) | 1721 |
| PdmI | GAANNNNTTC | 1 cut(s) | 2012 |
| PfeI | GAWTC | 7 cut(s) | 274, 431, 788, 1268, 1676, 1811, 2336 |
| PkrI | GCNGC | 7 cut(s) | 371, 515, 956, 1071, 1518, 1551, 1666 |
| Ple19I | CGATCG | 1 cut(s) | 2566 |
| PleI | GAGTC | 5 cut(s) | 165, 232, 275, 1309, 2441 |
| PpsI | GAGTC | 5 cut(s) | 165, 232, 275, 1309, 2441 |
| Ppu21I | YACGTR | 3 cut(s) | 635, 1859, 2255 |
| PpuMI | RGGWCCY | 1 cut(s) | 683 |
| PshBI | ATTAAT | 1 cut(s) | 1535 |
| PsiI | TTATAA | 1 cut(s) | 1700 |
| Psp124BI | GAGCTC | 1 cut(s) | 725 |
| Psp5II | RGGWCCY | 1 cut(s) | 683 |
| Psp6I | CCWGG | 4 cut(s) | 646, 1473, 2274, 2453 |
| PspEI | GGTNACC | 1 cut(s) | 1106 |
| PspGI | CCWGG | 4 cut(s) | 646, 1473, 2274, 2453 |
| PspN4I | GGNNCC | 5 cut(s) | 862, 1413, 1718, 2028, 2475 |
| PspPI | GGNCC | 3 cut(s) | 683, 861, 1693 |
| PspPPI | RGGWCCY | 1 cut(s) | 683 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 52, 84 |
| PstI | CTGCAG | 1 cut(s) | 799 |
| PstNI | CAGNNNCTG | 1 cut(s) | 995 |
| PsuI | RGATCY | 2 cut(s) | 425, 1201 |
| PvuI | CGATCG | 1 cut(s) | 2566 |
| PvuII | CAGCTG | 1 cut(s) | 1516 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1578, 1875 |
| SacI | GAGCTC | 1 cut(s) | 725 |
| SatI | GCNGC | 7 cut(s) | 370, 514, 955, 1070, 1517, 1550, 1665 |
| Sau96I | GGNCC | 3 cut(s) | 683, 861, 1693 |
| ScaI | AGTACT | 4 cut(s) | 61, 2124, 2289, 2510 |
| SchI | GAGTC | 5 cut(s) | 165, 232, 276, 1310, 2441 |
| ScrFI | CCNGG | 4 cut(s) | 648, 1475, 2276, 2455 |
| SduI | GDGCHC | 2 cut(s) | 146, 725 |
| SexAI | ACCWGGT | 1 cut(s) | 1473 |
| SfcI | CTRYAG | 3 cut(s) | 687, 795, 1086 |
| SfuI | TTCGAA | 1 cut(s) | 2169 |
| SinI | GGWCC | 2 cut(s) | 683, 861 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1578, 1875 |
| SmlI | CTYRAG | 2 cut(s) | 496, 2291 |
| SmoI | CTYRAG | 2 cut(s) | 496, 2291 |
| SnaBI | TACGTA | 1 cut(s) | 2255 |
| SphI | GCATGC | 1 cut(s) | 1069 |
| SseBI | AGGCCT | 1 cut(s) | 508 |
| SsiI | CCGC | 2 cut(s) | 369, 891 |
| SspMI | CTAG | 8 cut(s) | 108, 156, 510, 1199, 1317, 1802, 1838, 2501 |
| SstI | GAGCTC | 1 cut(s) | 725 |
| StuI | AGGCCT | 1 cut(s) | 508 |
| StyD4I | CCNGG | 4 cut(s) | 646, 1473, 2274, 2453 |
| StyI | CCWWGG | 1 cut(s) | 503 |
| TaiI | ACGT | 4 cut(s) | 637, 771, 1861, 2257 |
| TaqI | TCGA | 4 cut(s) | 382, 725, 1936, 2169 |
| TatI | WGTACW | 6 cut(s) | 59, 983, 2122, 2287, 2479, 2508 |
| TauI | GCSGC | 1 cut(s) | 372 |
| TfiI | GAWTC | 7 cut(s) | 274, 431, 788, 1268, 1676, 1811, 2336 |
| TseFI | GTSAC | 2 cut(s) | 1106, 1477 |
| TseI | GCWGC | 6 cut(s) | 513, 954, 1069, 1516, 1549, 1664 |
| Tsp45I | GTSAC | 2 cut(s) | 1106, 1477 |
| TspDTI | ATGAA | 7 cut(s) | 17, 1617, 1725, 1803, 1821, 2051, 2258 |
| TspGWI | ACGGA | 4 cut(s) | 884, 996, 1763, 2086 |
| Vha464I | CTTAAG | 1 cut(s) | 2291 |
| VpaK11BI | GGWCC | 2 cut(s) | 683, 861 |
| VspI | ATTAAT | 1 cut(s) | 1535 |
| XapI | RAATTY | 7 cut(s) | 183, 286, 446, 558, 1091, 1786, 2518 |
| XbaI | TCTAGA | 2 cut(s) | 1198, 1837 |
| XceI | RCATGY | 3 cut(s) | 900, 1069, 1122 |
| XmnI | GAANNNNTTC | 1 cut(s) | 2012 |
| XspI | CTAG | 8 cut(s) | 108, 156, 510, 1199, 1317, 1802, 1838, 2501 |
| ZrmI | AGTACT | 4 cut(s) | 61, 2124, 2289, 2510 |