Prupe.8G252500_v2.0.a1

monooxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0001076
Physical Location & Seq
Reverse (-)
21655932 .. 21658120
2189 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G252500.2

Sequence Viewer

Length: 1155 bp
ATGGAGCAGGAGAAGGCAGTGATTATAGTCGGGGCTGGCCCGTCCGGCCTCGCCATGGCCGGATGTCTAAGCCGGCTTGCAATCCCATACATAGTCTTGGAAAGAGAGGACTGTTTTGCTTCCATGTGGAAAAAGTATTCATATGACCGTCTCCACCTCCACCTTCAGAAGCAGTTCTGTGAGCTTCCCCACATGCCATTTCCACCTTCTTGTCCCACCTACGTGCCCAAGAATCAGTTTATCCAATACTTGGACGACTATGTGTCCCGTTTCAAGATTAGTCCCATCCTGTACCACAGAAATGTTGAGTCTGCAAACTATGATGAAGCTTCTGAGAGATGGGTTGTGAAGGCGGTGACTGATAGTAATACTGGTGGCTCTGGTGAGATGGAGGAGTATTTTGGGAGGTTTTTGGTGGTGGCTACAGGGGAAGCAACCAACCCATATACACCAGAGGTTCAAGGTTTGAACACTTTTGCTGGGGATGTTCTTCACTCAACTCAGTTCAAATCTGGGAAGGGGTTCAGAAACAAGAACGTTTTGGTTGTTGGGTCTGGGAATTCCGGCATGGAAATCGCTCTAGACCTAGCAAACCATGGTGCAAAGACTTCCATCATCGTTCGAAGCCCGGTCCATGTTTTGTCAAGGGGGATGGTTTACTTGGCCCTGGTTTTGTTGAAACATTTTTCGCTAAACATGGTGGACTCTTTGATGGTTTTGCTTAGCAAGCTGGTCTTTGGAGACTTAACCAAGTATGGGATAGAGAGGCCAACAGAGGGTCCCTTTTATATGAAGGTCAAGTACGGCAAGTATCCAGCCATTGATGTTGGAACCTTTAAGAAGATCAAGTCCAGTGAGATTCAGGTCTTGCCAGCAGAAATCAGCAGCATAAGAGGCAGCGATGTTGAATTGAAAAACGGCAAGTCGTACCAATTTGATGCAATTGTTTTCTGCACCGGGTTCAAGAGATCAACGAACTTGTGGCTCAAGGGGGATGATTATCTTTTAAAAGATGATGGACTTCCAAGACAAAGTTTCCCCAACCATTGGAAGGGAAAGAACGGTTTATATTGTGTGGGATTATCAAGAAGAGGAATATATGGGTCTAGTGAGGATGCTCAAAACATAGCAAATGATATCAAGTCATTTCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00380 ko01100 map00380 map01100
Pfam Domains
Protein Families
FMO-like K_oxygenase Pyr_redox_3

Protein Analysis

385

Amino Acids

42.87

Weight (kDa)

8.67

Isoelectric Point (pI)

38.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 250, 1047
AciI CCGC 1 cut(s) 353
AclI AACGTT 1 cut(s) 537
AcoI YGGCCR 1 cut(s) 57
AcsI RAATTY 1 cut(s) 559
AcuI CTGAAG 1 cut(s) 149
AfaI GTAC 3 cut(s) 293, 803, 929
AfiI CCNNNNNNNGG 6 cut(s) 55, 250, 756, 776, 1047, 1051
AgsI TTSAA 8 cut(s) 274, 461, 469, 508, 679, 908, 913, 964
AhdI GACNNNNNGTC 1 cut(s) 262
AjnI CCWGG 1 cut(s) 666
AleI CACNNNNGTG 1 cut(s) 221
AluBI AGCT 3 cut(s) 184, 329, 730
AluI AGCT 3 cut(s) 184, 329, 730
Alw26I GTCTC 2 cut(s) 155, 735
AoxI GGCC 5 cut(s) 37, 46, 57, 663, 767
ApeKI GCWGC 2 cut(s) 885, 897
ApoI RAATTY 1 cut(s) 559
Asp700I GAANNNNTTC 2 cut(s) 173, 521
AspS9I GGNCC 4 cut(s) 38, 631, 664, 779
AsuC2I CCSGG 2 cut(s) 629, 958
AsuHPI GGTGA 2 cut(s) 367, 395
AsuII TTCGAA 1 cut(s) 622
AvaII GGWCC 2 cut(s) 631, 779
BaeGI GKGCMC 1 cut(s) 228
BarI GAAGNNNNNNTAC 2 cut(s) 785, 817
BbvI GCAGC 2 cut(s) 897, 909
BccI CCATC 7 cut(s) 293, 333, 382, 620, 646, 706, 1010
BceAI ACGGC 2 cut(s) 820, 934
BcgI CGANNNNNNTGC 2 cut(s) 556, 590
BciT130I CCWGG 1 cut(s) 668
BciVI GTATCC 1 cut(s) 822
BcnI CCSGG 2 cut(s) 629, 958
BcoDI GTCTC 2 cut(s) 155, 735
BfaI CTAG 3 cut(s) 581, 587, 1107
BfmI CTRYAG 2 cut(s) 423, 1151
BfuI GTATCC 1 cut(s) 822
BglI GCCNNNNNGGC 1 cut(s) 45
BisI GCNGC 2 cut(s) 886, 898
BlpI GCTNAGC 1 cut(s) 722
BlsI GCNGC 2 cut(s) 887, 899
Bme1390I CCNGG 3 cut(s) 629, 668, 958
Bme18I GGWCC 2 cut(s) 631, 779
BmeRI GACNNNNNGTC 1 cut(s) 262
BmgT120I GGNCC 4 cut(s) 38, 631, 664, 779
BmiI GGNNCC 3 cut(s) 780, 781, 832
BmrFI CCNGG 3 cut(s) 629, 668, 958
BmsI GCATC 2 cut(s) 928, 1105
Bpu1102I GCTNAGC 1 cut(s) 722
Bpu14I TTCGAA 1 cut(s) 622
BpuEI CTTGAG 1 cut(s) 971
BpuMI CCSGG 2 cut(s) 629, 958
BsaAI YACGTR 1 cut(s) 223
BsaBI GATNNNNATC 1 cut(s) 999
BsaJI CCNNGG 3 cut(s) 54, 595, 666
Bsc4I CCNNNNNNNGG 6 cut(s) 55, 250, 756, 776, 1047, 1051
Bse118I RCCGGY 1 cut(s) 72
Bse1I ACTGG 2 cut(s) 376, 852
Bse8I GATNNNNATC 1 cut(s) 999
BseBI CCWGG 1 cut(s) 668
BseDI CCNNGG 3 cut(s) 54, 595, 666
BseGI GGATG 6 cut(s) 68, 285, 490, 657, 1000, 1120
BseJI GATNNNNATC 1 cut(s) 999
BseLI CCNNNNNNNGG 6 cut(s) 55, 250, 756, 776, 1047, 1051
BseMII CTCAG 2 cut(s) 324, 515
BseNI ACTGG 2 cut(s) 376, 852
BseRI GAGGAG 1 cut(s) 407
BseSI GKGCMC 1 cut(s) 228
BseXI GCAGC 2 cut(s) 897, 909
BseYI CCCAGC 1 cut(s) 479
BsgI GTGCAG 1 cut(s) 937
BshFI GGCC 5 cut(s) 39, 48, 59, 665, 769
BsiSI CCGG 6 cut(s) 45, 60, 73, 564, 629, 957
BslFI GGGAC 4 cut(s) 198, 250, 267, 765
BslI CCNNNNNNNGG 6 cut(s) 55, 250, 756, 776, 1047, 1051
BsmAI GTCTC 2 cut(s) 155, 735
BsmBI CGTCTC 1 cut(s) 155
BsmFI GGGAC 4 cut(s) 198, 250, 267, 765
BsnI GGCC 5 cut(s) 39, 48, 59, 665, 769
Bsp119I TTCGAA 1 cut(s) 622
Bsp1286I GDGCHC 1 cut(s) 228
Bsp143I GATC 2 cut(s) 843, 968
Bsp1720I GCTNAGC 1 cut(s) 722
Bsp19I CCATGG 2 cut(s) 54, 595
BspACI CCGC 1 cut(s) 353
BspANI GGCC 5 cut(s) 39, 48, 59, 665, 769
BspCNI CTCAG 2 cut(s) 325, 514
BspLI GGNNCC 3 cut(s) 780, 781, 832
BspT104I TTCGAA 1 cut(s) 622
BsrFI RCCGGY 1 cut(s) 72
BsrI ACTGG 2 cut(s) 376, 852
BssAI RCCGGY 1 cut(s) 72
BssECI CCNNGG 3 cut(s) 54, 595, 666
BssMI GATC 2 cut(s) 843, 968
BssT1I CCWWGG 2 cut(s) 54, 595
Bst2UI CCWGG 1 cut(s) 668
Bst4CI ACNGT 3 cut(s) 113, 149, 1064
Bst6I CTCTTC 1 cut(s) 1084
BstBAI YACGTR 1 cut(s) 223
BstBI TTCGAA 1 cut(s) 622
BstC8I GCNNGC 5 cut(s) 37, 74, 78, 728, 873
BstDEI CTNAG 4 cut(s) 68, 333, 501, 722
BstDSI CCRYGG 2 cut(s) 54, 595
BstF5I GGATG 6 cut(s) 68, 285, 490, 657, 1000, 1120
BstKTI GATC 2 cut(s) 846, 971
BstMAI GTCTC 2 cut(s) 155, 735
BstMBI GATC 2 cut(s) 843, 968
BstMWI GCNNNNNNNGC 3 cut(s) 45, 727, 894
BstNI CCWGG 1 cut(s) 668
BstNSI RCATGY 1 cut(s) 196
BstSCI CCNGG 3 cut(s) 627, 666, 956
BstSFI CTRYAG 2 cut(s) 423, 1151
BstSLI GKGCMC 1 cut(s) 228
BstV1I GCAGC 2 cut(s) 897, 909
BsuI GTATCC 1 cut(s) 822
BsuRI GGCC 5 cut(s) 39, 48, 59, 665, 769
BtgI CCRYGG 2 cut(s) 54, 595
BtgZI GCGATG 1 cut(s) 915
BtsCI GGATG 6 cut(s) 68, 285, 490, 657, 1000, 1120
BtsI GCAGTG 1 cut(s) 24
BtsIMutI CAGTG 2 cut(s) 24, 859
Cac8I GCNNGC 5 cut(s) 37, 74, 78, 728, 873
Cfr10I RCCGGY 1 cut(s) 72
Cfr13I GGNCC 4 cut(s) 38, 631, 664, 779
Csp6I GTAC 3 cut(s) 292, 802, 928
CviAII CATG 7 cut(s) 55, 124, 193, 568, 596, 635, 697
CviQI GTAC 3 cut(s) 292, 802, 928
DdeI CTNAG 4 cut(s) 68, 333, 501, 722
DpnI GATC 2 cut(s) 845, 970
DpnII GATC 2 cut(s) 843, 968
DraI TTTAAA 1 cut(s) 1008
DriI GACNNNNNGTC 1 cut(s) 262
EaeI YGGCCR 1 cut(s) 57
Eam1104I CTCTTC 1 cut(s) 1084
Eam1105I GACNNNNNGTC 1 cut(s) 262
EarI CTCTTC 1 cut(s) 1084
Eco130I CCWWGG 2 cut(s) 54, 595
Eco32I GATATC 1 cut(s) 1138
Eco47I GGWCC 2 cut(s) 631, 779
Eco57I CTGAAG 1 cut(s) 149
EcoO109I RGGNCCY 1 cut(s) 779
EcoRI GAATTC 1 cut(s) 559
EcoRII CCWGG 1 cut(s) 666
EcoRV GATATC 1 cut(s) 1138
EcoT14I CCWWGG 2 cut(s) 54, 595
ErhI CCWWGG 2 cut(s) 54, 595
Esp3I CGTCTC 1 cut(s) 155
FaeI CATG 7 cut(s) 58, 127, 196, 571, 599, 638, 700
FalI AAGNNNNNCTT 2 cut(s) 719, 751
FaqI GGGAC 4 cut(s) 198, 250, 267, 765
FatI CATG 7 cut(s) 54, 123, 192, 567, 595, 634, 696
FauNDI CATATG 1 cut(s) 142
Fnu4HI GCNGC 2 cut(s) 886, 898
FokI GGATG 6 cut(s) 75, 272, 497, 664, 1007, 1127
Fsp4HI GCNGC 2 cut(s) 886, 898
FspBI CTAG 3 cut(s) 581, 587, 1107
GluI GCNGC 2 cut(s) 886, 898
GsaI CCCAGC 1 cut(s) 483
HaeIII GGCC 5 cut(s) 39, 48, 59, 665, 769
HapII CCGG 6 cut(s) 45, 60, 73, 564, 629, 957
Hin1II CATG 7 cut(s) 58, 127, 196, 571, 599, 638, 700
HindIII AAGCTT 1 cut(s) 327
HinfI GANTC 4 cut(s) 232, 308, 704, 859
HpaII CCGG 6 cut(s) 45, 60, 73, 564, 629, 957
HphI GGTGA 2 cut(s) 367, 395
Hpy166II GTNNAC 2 cut(s) 658, 703
Hpy188I TCNGA 3 cut(s) 168, 334, 527
Hpy188III TCNNGA 4 cut(s) 274, 581, 964, 1086
Hpy8I GTNNAC 2 cut(s) 658, 703
HpyAV CCTTC 7 cut(s) 7, 173, 216, 343, 511, 787, 1045
HpyCH4III ACNGT 3 cut(s) 113, 149, 1064
HpyCH4IV ACGT 2 cut(s) 222, 537
HpyCH4V TGCA 5 cut(s) 80, 314, 602, 941, 954
HpyF10VI GCNNNNNNNGC 3 cut(s) 45, 727, 894
HpyF3I CTNAG 4 cut(s) 68, 333, 501, 722
HpySE526I ACGT 2 cut(s) 222, 537
Hsp92II CATG 7 cut(s) 58, 127, 196, 571, 599, 638, 700
KflI GGGWCCC 1 cut(s) 779
KroI GCCGGC 1 cut(s) 72
KroNI GCCGGC 1 cut(s) 74
Kzo9I GATC 2 cut(s) 843, 968
LmnI GCTCC 1 cut(s) 4
Lsp1109I GCAGC 2 cut(s) 897, 909
LweI GCATC 2 cut(s) 928, 1105
MaeI CTAG 3 cut(s) 581, 587, 1107
MaeII ACGT 2 cut(s) 222, 537
MaeIII GTNAC 1 cut(s) 355
MalI GATC 2 cut(s) 845, 970
MboI GATC 2 cut(s) 843, 968
MboII GAAGA 3 cut(s) 482, 853, 1101
MfeI CAATTG 1 cut(s) 942
MhlI GDGCHC 1 cut(s) 228
MluCI AATT 4 cut(s) 559, 908, 932, 942
MlyI GAGTC 2 cut(s) 317, 698
MmeI TCCRAC 1 cut(s) 808
MroNI GCCGGC 1 cut(s) 72
MroXI GAANNNNTTC 2 cut(s) 173, 521
MseI TTAA 3 cut(s) 746, 837, 1007
MslI CAYNNNNRTG 2 cut(s) 221, 300
MspI CCGG 6 cut(s) 45, 60, 73, 564, 629, 957
MspR9I CCNGG 3 cut(s) 629, 668, 958
MunI CAATTG 1 cut(s) 942
MvaI CCWGG 1 cut(s) 668
MwoI GCNNNNNNNGC 3 cut(s) 45, 727, 894
NaeI GCCGGC 1 cut(s) 74
NciI CCSGG 2 cut(s) 629, 958
NcoI CCATGG 2 cut(s) 54, 595
NdeI CATATG 1 cut(s) 142
NdeII GATC 2 cut(s) 843, 968
NgoMIV GCCGGC 1 cut(s) 72
NlaIII CATG 7 cut(s) 58, 127, 196, 571, 599, 638, 700
NlaIV GGNNCC 3 cut(s) 780, 781, 832
NmuCI GTSAC 1 cut(s) 355
NspI RCATGY 1 cut(s) 196
NspV TTCGAA 1 cut(s) 622
OliI CACNNNNGTG 1 cut(s) 221
PdiI GCCGGC 1 cut(s) 74
PdmI GAANNNNTTC 2 cut(s) 173, 521
PfeI GAWTC 2 cut(s) 232, 859
PflMI CCANNNNNTGG 2 cut(s) 250, 1047
PkrI GCNGC 2 cut(s) 887, 899
PleI GAGTC 2 cut(s) 316, 698
PpsI GAGTC 2 cut(s) 316, 698
Ppu21I YACGTR 1 cut(s) 223
PpuMI RGGWCCY 1 cut(s) 779
Psp1406I AACGTT 1 cut(s) 537
Psp5II RGGWCCY 1 cut(s) 779
Psp6I CCWGG 1 cut(s) 666
PspFI CCCAGC 1 cut(s) 479
PspGI CCWGG 1 cut(s) 666
PspN4I GGNNCC 3 cut(s) 780, 781, 832
PspPI GGNCC 4 cut(s) 38, 631, 664, 779
PspPPI RGGWCCY 1 cut(s) 779
RsaI GTAC 3 cut(s) 293, 803, 929
RsaNI GTAC 3 cut(s) 292, 802, 928
RseI CAYNNNNRTG 2 cut(s) 221, 300
SaqAI TTAA 3 cut(s) 746, 837, 1007
SatI GCNGC 2 cut(s) 886, 898
Sau3AI GATC 2 cut(s) 843, 968
Sau96I GGNCC 4 cut(s) 38, 631, 664, 779
SchI GAGTC 2 cut(s) 317, 698
ScrFI CCNGG 3 cut(s) 629, 668, 958
SduI GDGCHC 1 cut(s) 228
SfaNI GCATC 2 cut(s) 928, 1105
SfcI CTRYAG 2 cut(s) 423, 1151
SfiI GGCCNNNNNGGCC 1 cut(s) 45
SfuI TTCGAA 1 cut(s) 622
SinI GGWCC 2 cut(s) 631, 779
SmiMI CAYNNNNRTG 2 cut(s) 221, 300
SmlI CTYRAG 1 cut(s) 986
SmoI CTYRAG 1 cut(s) 986
Sse9I AATT 4 cut(s) 559, 908, 932, 942
SsiI CCGC 1 cut(s) 353
SspMI CTAG 3 cut(s) 581, 587, 1107
StyD4I CCNGG 3 cut(s) 627, 666, 956
StyI CCWWGG 2 cut(s) 54, 595
TaaI ACNGT 3 cut(s) 113, 149, 1064
TaiI ACGT 2 cut(s) 225, 540
TaqI TCGA 1 cut(s) 622
TasI AATT 4 cut(s) 559, 908, 932, 942
TfiI GAWTC 2 cut(s) 232, 859
Tru1I TTAA 3 cut(s) 746, 837, 1007
Tru9I TTAA 3 cut(s) 746, 837, 1007
TscAI CASTG 2 cut(s) 24, 859
TseFI GTSAC 1 cut(s) 355
TseI GCWGC 2 cut(s) 885, 897
Tsp45I GTSAC 1 cut(s) 355
TspDTI ATGAA 3 cut(s) 129, 339, 806
TspRI CASTG 2 cut(s) 24, 859
Van91I CCANNNNNTGG 2 cut(s) 250, 1047
VpaK11BI GGWCC 2 cut(s) 631, 779
XapI RAATTY 1 cut(s) 559
XbaI TCTAGA 1 cut(s) 580
XceI RCATGY 1 cut(s) 196
XmnI GAANNNNTTC 2 cut(s) 173, 521
XspI CTAG 3 cut(s) 581, 587, 1107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.