Rorug06G0062000

monooxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0001076
Physical Location & Seq
Reverse (-)
8278190 .. 8280385
2196 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0062000.1

Sequence Viewer

Length: 1143 bp
ATGCTGAATAACTACTTCAAGAAAAAGATTGATCCAGACGATGCACATGCCAAGTACCAGAGCGTGGCCCTGGTACTTGGGGTCACAGGAATCGTCGGAAACAGCCTTGCTGAGATTCTTCCACTATCCGACACTCCTGGCGGGCCGTGTAAGGTCTACGGAGTGGCCCGGCGGCCCCGGCCGCAGTGGAACGCTGACCACCCGATTCAGTACATCCAGTGTGACCTCTTAGACTCCAAACAGACAGAGGCAGAGCTCTCCCAGCTCACTGATGTCACTCACATTTTTTATGTCACATGGGCGAGCAAGCCAACTGAAGCTGAGAACTGTGACGCTAATGGCAAGATGTTAAGCAATGTGTTGAATGCTGTGATCCCAAACGCGCCGAATTTGCAGCACATATGTATGCAGACGGGTCGGAAGCACTACTTAGGTTCGGTTGAGATGTTGGGGAAAGTCCAACCCCATGAACCTCCATATCACGAAGACCTGCCGAGGCTTAATGCCCCCAATTTTTACTATGTGATGGAGGATATTCTGTTTGATGAGGTGAAGAAGGAGGGGCTGACATGGTCTGTACACAGGCCGGGAGTCATATTCGGGTTCTCACCTTATAGCTTGATGAATGCGATTGGGAGTTTGTGTGTTTACGCTGCGATTTGTAAGCATGAAGGGCAGCCGTTGAGGTTTCCGGGGAGTAAAGGAACTTGGGAAGGGTTCTGGGATGTGTCTGATGCGGATATGATTGCTGAACATCAGATATGGGCAGCTGTGGAGCCTTATGCCAAGAAAGAAGCTTTTAATTGCAGCAATGGGGATGTATTCAAGTGGAAGCATCTGTGGGGGCTTTTGGCTGAGCAGTTTGAGGTAGTGCCTGCTGGAGTTCACGAGGAGTTGAGTTTTGAGGAGATGATGAAAGATAATGGGCCAGTGTGGAATGAAATTGTAAGAGAGCATGGTTTGGTGCCTACCAAATTGGAAGACGTAGGGAATTGGTTGTTTCTGGACATTATATTTCAGGATGATTCAACTGTGGACAGTATGAACAAGAGCAAAGAGCATGGTTTTGTTGGCTTTAGGAACTCGAAAACTTCCTTTGTTTCTTGGATTGACAAGATGAAGTCTTTTAGAATTGTTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00380 ko01100 map00380 map01100
Pfam Domains
Protein Families
FMO-like K_oxygenase Pyr_redox_3

Protein Analysis

380

Amino Acids

42.93

Weight (kDa)

5.45

Isoelectric Point (pI)

32.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRISE PF22917 23 - 380 5.9e-27 PRISE-like Rossmann-fold domain
Epimerase PF01370 23 - 182 4.6e-09 NAD dependent epimerase/dehydratase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 498
AccB1I GGYRCC 1 cut(s) 964
AccB7I CCANNNNNTGG 1 cut(s) 64
AccI GTMKAC 1 cut(s) 156
AccII CGCG 1 cut(s) 383
AciI CCGC 4 cut(s) 141, 172, 182, 737
AclWI GGATC 2 cut(s) 26, 367
AcoI YGGCCR 1 cut(s) 179
AcsI RAATTY 1 cut(s) 388
AcuI CTGAAG 1 cut(s) 336
AfaI GTAC 4 cut(s) 56, 75, 212, 579
AfiI CCNNNNNNNGG 1 cut(s) 64
AgsI TTSAA 4 cut(s) 19, 364, 826, 1029
AjnI CCWGG 2 cut(s) 69, 136
AluBI AGCT 6 cut(s) 256, 265, 320, 618, 770, 797
AluI AGCT 6 cut(s) 256, 265, 320, 618, 770, 797
Alw21I GWGCWC 1 cut(s) 258
AlwI GGATC 2 cut(s) 26, 367
AoxI GGCC 7 cut(s) 66, 143, 165, 173, 179, 584, 926
ApeKI GCWGC 5 cut(s) 394, 653, 676, 767, 807
ApoI RAATTY 1 cut(s) 388
AspLEI GCGC 1 cut(s) 385
AspS9I GGNCC 5 cut(s) 67, 143, 166, 174, 926
AsuC2I CCSGG 4 cut(s) 169, 178, 588, 693
AsuHPI GGTGA 2 cut(s) 562, 600
BanI GGYRCC 1 cut(s) 964
BanII GRGCYC 1 cut(s) 258
BauI CACGAG 1 cut(s) 887
BbsI GAAGAC 2 cut(s) 492, 987
Bbv12I GWGCWC 1 cut(s) 258
BbvI GCAGC 5 cut(s) 406, 640, 688, 779, 819
BccI CCATC 1 cut(s) 520
BceAI ACGGC 2 cut(s) 130, 664
BcgI CGANNNNNNTGC 4 cut(s) 29, 63, 398, 432
BciT130I CCWGG 2 cut(s) 71, 138
BcnI CCSGG 4 cut(s) 169, 178, 588, 693
BfuAI ACCTGC 1 cut(s) 498
BisI GCNGC 7 cut(s) 173, 182, 395, 654, 677, 768, 808
BlpI GCTNAGC 1 cut(s) 855
BlsI GCNGC 7 cut(s) 174, 183, 396, 655, 678, 769, 809
Bme1390I CCNGG 6 cut(s) 71, 138, 169, 178, 588, 693
BmgT120I GGNCC 5 cut(s) 67, 143, 166, 174, 926
BmiI GGNNCC 3 cut(s) 176, 777, 966
BmrFI CCNGG 6 cut(s) 71, 138, 169, 178, 588, 693
BmsI GCATC 3 cut(s) 31, 724, 844
BpiI GAAGAC 2 cut(s) 492, 987
BpmI CTGGAG 1 cut(s) 900
Bpu1102I GCTNAGC 1 cut(s) 855
BpuMI CCSGG 4 cut(s) 169, 178, 588, 693
BsaJI CCNNGG 4 cut(s) 69, 176, 494, 692
BsaXI ACNNNNNCTCC 2 cut(s) 628, 658
Bsc4I CCNNNNNNNGG 1 cut(s) 64
Bse1I ACTGG 2 cut(s) 217, 929
Bse3DI GCAATG 2 cut(s) 361, 817
BseBI CCWGG 2 cut(s) 71, 138
BseDI CCNNGG 4 cut(s) 69, 176, 494, 692
BseGI GGATG 4 cut(s) 213, 730, 823, 1027
BseLI CCNNNNNNNGG 1 cut(s) 64
BseMI GCAATG 2 cut(s) 361, 817
BseMII CTCAG 3 cut(s) 102, 312, 846
BseNI ACTGG 2 cut(s) 217, 929
BseRI GAGGAG 2 cut(s) 905, 920
BseX3I CGGCCG 1 cut(s) 179
BseXI GCAGC 5 cut(s) 406, 640, 688, 779, 819
BseYI CCCAGC 1 cut(s) 261
Bsh1236I CGCG 1 cut(s) 383
Bsh1285I CGRYCG 1 cut(s) 182
BshFI GGCC 7 cut(s) 68, 145, 167, 175, 181, 586, 928
BshNI GGYRCC 1 cut(s) 964
BsiEI CGRYCG 1 cut(s) 182
BsiHKAI GWGCWC 1 cut(s) 258
BsiSI CCGG 4 cut(s) 169, 178, 587, 692
BslI CCNNNNNNNGG 1 cut(s) 64
BsmI GAATGC 2 cut(s) 370, 631
BsnI GGCC 7 cut(s) 68, 145, 167, 175, 181, 586, 928
Bsp1286I GDGCHC 1 cut(s) 258
Bsp1407I TGTACA 1 cut(s) 577
Bsp143I GATC 2 cut(s) 31, 372
Bsp1720I GCTNAGC 1 cut(s) 855
BspACI CCGC 4 cut(s) 141, 172, 182, 737
BspANI GGCC 7 cut(s) 68, 145, 167, 175, 181, 586, 928
BspCNI CTCAG 3 cut(s) 103, 313, 847
BspFNI CGCG 1 cut(s) 383
BspLI GGNNCC 3 cut(s) 176, 777, 966
BspMI ACCTGC 1 cut(s) 498
BspPI GGATC 2 cut(s) 26, 367
BspT107I GGYRCC 1 cut(s) 964
BsrDI GCAATG 2 cut(s) 361, 817
BsrGI TGTACA 1 cut(s) 577
BsrI ACTGG 2 cut(s) 217, 929
BssECI CCNNGG 4 cut(s) 69, 176, 494, 692
BssMI GATC 2 cut(s) 31, 372
BssSI CACGAG 1 cut(s) 887
Bst2BI CACGAG 1 cut(s) 887
Bst2UI CCWGG 2 cut(s) 71, 138
Bst4CI ACNGT 3 cut(s) 329, 1033, 1040
BstAUI TGTACA 1 cut(s) 577
BstC8I GCNNGC 4 cut(s) 143, 304, 308, 876
BstDEI CTNAG 5 cut(s) 111, 229, 321, 430, 855
BstF5I GGATG 4 cut(s) 213, 730, 823, 1027
BstFNI CGCG 1 cut(s) 383
BstHHI GCGC 1 cut(s) 385
BstKTI GATC 2 cut(s) 34, 375
BstMBI GATC 2 cut(s) 31, 372
BstMCI CGRYCG 1 cut(s) 182
BstMWI GCNNNNNNNGC 5 cut(s) 178, 181, 262, 391, 673
BstNI CCWGG 2 cut(s) 71, 138
BstNSI RCATGY 1 cut(s) 50
BstSCI CCNGG 6 cut(s) 69, 136, 167, 176, 586, 691
BstUI CGCG 1 cut(s) 383
BstV1I GCAGC 5 cut(s) 406, 640, 688, 779, 819
BstV2I GAAGAC 2 cut(s) 492, 987
BstZI CGGCCG 1 cut(s) 179
BsuRI GGCC 7 cut(s) 68, 145, 167, 175, 181, 586, 928
BtsCI GGATG 4 cut(s) 213, 730, 823, 1027
BtsI GCAGTG 1 cut(s) 191
BtsIMutI CAGTG 4 cut(s) 191, 224, 267, 936
BveI ACCTGC 1 cut(s) 498
Cac8I GCNNGC 4 cut(s) 143, 304, 308, 876
CfoI GCGC 1 cut(s) 385
Cfr13I GGNCC 5 cut(s) 67, 143, 166, 174, 926
CseI GACGC 1 cut(s) 341
Csp6I GTAC 4 cut(s) 55, 74, 211, 578
CviAII CATG 7 cut(s) 47, 297, 467, 570, 668, 956, 1061
CviQI GTAC 4 cut(s) 55, 74, 211, 578
DdeI CTNAG 5 cut(s) 111, 229, 321, 430, 855
DpnI GATC 2 cut(s) 33, 374
DpnII GATC 2 cut(s) 31, 372
EaeI YGGCCR 1 cut(s) 179
EagI CGGCCG 1 cut(s) 179
Ecl136II GAGCTC 1 cut(s) 256
EclXI CGGCCG 1 cut(s) 179
Eco24I GRGCYC 1 cut(s) 258
Eco52I CGGCCG 1 cut(s) 179
Eco53kI GAGCTC 1 cut(s) 256
Eco57I CTGAAG 1 cut(s) 336
EcoICRI GAGCTC 1 cut(s) 256
EcoRII CCWGG 2 cut(s) 69, 136
EcoT38I GRGCYC 1 cut(s) 258
FaeI CATG 7 cut(s) 50, 300, 470, 573, 671, 959, 1064
FalI AAGNNNNNCTT 2 cut(s) 413, 445
FatI CATG 7 cut(s) 46, 296, 466, 569, 667, 955, 1060
FauI CCCGC 1 cut(s) 134
FauNDI CATATG 1 cut(s) 401
FblI GTMKAC 1 cut(s) 156
Fnu4HI GCNGC 7 cut(s) 173, 182, 395, 654, 677, 768, 808
FokI GGATG 4 cut(s) 200, 737, 830, 1034
FriOI GRGCYC 1 cut(s) 258
Fsp4HI GCNGC 7 cut(s) 173, 182, 395, 654, 677, 768, 808
GlaI GCGC 1 cut(s) 384
GluI GCNGC 7 cut(s) 173, 182, 395, 654, 677, 768, 808
GsaI CCCAGC 1 cut(s) 265
GsuI CTGGAG 1 cut(s) 900
HaeIII GGCC 7 cut(s) 68, 145, 167, 175, 181, 586, 928
HapII CCGG 4 cut(s) 169, 178, 587, 692
HgaI GACGC 1 cut(s) 341
HhaI GCGC 1 cut(s) 385
Hin1II CATG 7 cut(s) 50, 300, 470, 573, 671, 959, 1064
Hin6I GCGC 1 cut(s) 383
HinP1I GCGC 1 cut(s) 383
HindIII AAGCTT 1 cut(s) 795
HinfI GANTC 6 cut(s) 90, 115, 205, 233, 591, 1025
HpaII CCGG 4 cut(s) 169, 178, 587, 692
HphI GGTGA 2 cut(s) 562, 600
Hpy166II GTNNAC 5 cut(s) 157, 580, 649, 886, 1036
Hpy188I TCNGA 5 cut(s) 98, 130, 420, 733, 759
Hpy188III TCNNGA 6 cut(s) 19, 35, 482, 887, 1004, 1019
Hpy8I GTNNAC 5 cut(s) 157, 580, 649, 886, 1036
Hpy99I CGWCG 1 cut(s) 98
HpyAV CCTTC 3 cut(s) 550, 665, 707
HpyCH4III ACNGT 3 cut(s) 329, 1033, 1040
HpyCH4IV ACGT 1 cut(s) 984
HpyCH4V TGCA 4 cut(s) 44, 394, 409, 807
HpyF10VI GCNNNNNNNGC 5 cut(s) 178, 181, 262, 391, 673
HpyF3I CTNAG 5 cut(s) 111, 229, 321, 430, 855
HpySE526I ACGT 1 cut(s) 984
Hsp92II CATG 7 cut(s) 50, 300, 470, 573, 671, 959, 1064
HspAI GCGC 1 cut(s) 383
Kzo9I GATC 2 cut(s) 31, 372
LmnI GCTCC 1 cut(s) 775
Lsp1109I GCAGC 5 cut(s) 406, 640, 688, 779, 819
LweI GCATC 3 cut(s) 31, 724, 844
MaeII ACGT 1 cut(s) 984
MaeIII GTNAC 5 cut(s) 82, 221, 274, 292, 329
MalI GATC 2 cut(s) 33, 374
MboI GATC 2 cut(s) 31, 372
MboII GAAGA 4 cut(s) 110, 497, 565, 992
MhlI GDGCHC 1 cut(s) 258
MluCI AATT 7 cut(s) 388, 511, 802, 942, 974, 991, 1131
MlyI GAGTC 2 cut(s) 227, 600
MmeI TCCRAC 4 cut(s) 76, 153, 398, 484
MseI TTAA 3 cut(s) 350, 501, 801
MslI CAYNNNNRTG 1 cut(s) 404
MspA1I CMGCKG 1 cut(s) 770
MspI CCGG 4 cut(s) 169, 178, 587, 692
MspR9I CCNGG 6 cut(s) 71, 138, 169, 178, 588, 693
Mva1269I GAATGC 2 cut(s) 370, 631
MvaI CCWGG 2 cut(s) 71, 138
MvnI CGCG 1 cut(s) 383
MwoI GCNNNNNNNGC 5 cut(s) 178, 181, 262, 391, 673
NciI CCSGG 4 cut(s) 169, 178, 588, 693
NdeI CATATG 1 cut(s) 401
NdeII GATC 2 cut(s) 31, 372
NlaIII CATG 7 cut(s) 50, 300, 470, 573, 671, 959, 1064
NlaIV GGNNCC 3 cut(s) 176, 777, 966
NmeAIII GCCGAG 1 cut(s) 519
NmuCI GTSAC 5 cut(s) 82, 221, 274, 292, 329
NspI RCATGY 1 cut(s) 50
PctI GAATGC 2 cut(s) 370, 631
PfeI GAWTC 4 cut(s) 90, 115, 205, 1025
PflFI GACNNNGTC 1 cut(s) 571
PflMI CCANNNNNTGG 1 cut(s) 64
PkrI GCNGC 7 cut(s) 174, 183, 396, 655, 678, 769, 809
PleI GAGTC 2 cut(s) 227, 599
PpsI GAGTC 2 cut(s) 227, 599
Psp124BI GAGCTC 1 cut(s) 258
Psp6I CCWGG 2 cut(s) 69, 136
PspFI CCCAGC 1 cut(s) 261
PspGI CCWGG 2 cut(s) 69, 136
PspN4I GGNNCC 3 cut(s) 176, 777, 966
PspPI GGNCC 5 cut(s) 67, 143, 166, 174, 926
PsyI GACNNNGTC 1 cut(s) 571
PvuII CAGCTG 1 cut(s) 770
RsaI GTAC 4 cut(s) 56, 75, 212, 579
RsaNI GTAC 4 cut(s) 55, 74, 211, 578
RseI CAYNNNNRTG 1 cut(s) 404
SacI GAGCTC 1 cut(s) 258
SaqAI TTAA 3 cut(s) 350, 501, 801
SatI GCNGC 7 cut(s) 173, 182, 395, 654, 677, 768, 808
Sau3AI GATC 2 cut(s) 31, 372
Sau96I GGNCC 5 cut(s) 67, 143, 166, 174, 926
SchI GAGTC 2 cut(s) 227, 600
ScrFI CCNGG 6 cut(s) 71, 138, 169, 178, 588, 693
SduI GDGCHC 1 cut(s) 258
SfaNI GCATC 3 cut(s) 31, 724, 844
SmiMI CAYNNNNRTG 1 cut(s) 404
Sse9I AATT 7 cut(s) 388, 511, 802, 942, 974, 991, 1131
SsiI CCGC 4 cut(s) 141, 172, 182, 737
SstI GAGCTC 1 cut(s) 258
StyD4I CCNGG 6 cut(s) 69, 136, 167, 176, 586, 691
TaaI ACNGT 3 cut(s) 329, 1033, 1040
TaiI ACGT 1 cut(s) 987
TaqI TCGA 1 cut(s) 1085
TasI AATT 7 cut(s) 388, 511, 802, 942, 974, 991, 1131
TatI WGTACW 2 cut(s) 210, 577
TauI GCSGC 2 cut(s) 175, 184
TfiI GAWTC 4 cut(s) 90, 115, 205, 1025
Tru1I TTAA 3 cut(s) 350, 501, 801
Tru9I TTAA 3 cut(s) 350, 501, 801
TscAI CASTG 4 cut(s) 191, 224, 274, 936
TseFI GTSAC 5 cut(s) 82, 221, 274, 292, 329
TseI GCWGC 5 cut(s) 394, 653, 676, 767, 807
Tsp45I GTSAC 5 cut(s) 82, 221, 274, 292, 329
TspDTI ATGAA 7 cut(s) 483, 638, 684, 929, 954, 1058, 1133
TspGWI ACGGA 1 cut(s) 174
TspRI CASTG 4 cut(s) 191, 224, 274, 936
Tth111I GACNNNGTC 1 cut(s) 571
Van91I CCANNNNNTGG 1 cut(s) 64
XapI RAATTY 1 cut(s) 388
XceI RCATGY 1 cut(s) 50
XmiI GTMKAC 1 cut(s) 156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.