Rroxscaffold_7G00196640

monooxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0001076
Physical Location & Seq
Forward (+)
39674340 .. 39678259
3920 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00196640.1

Sequence Viewer

Length: 1146 bp
ATGCCGGAAGCAGTGGTGATAATAGTTGGGGCGGGCCCGTCCGGCCTCGCGATGGCCGGCTGTCTCAGCCGGCTCGAAATCCCATACATATTTCTCGAAAGAGAGGACTGTTATGCTCCTCTTTGGAAGAAGTACTCCTACGACCGTCTCCACCTTCACCTTCATAAGCAGGTCTGTGAGCTCCCCCACATGTCATTCCCGTCCTCTTATCCCACGTATGTACCCAAGAAACAGTTCCTTCAGTACTTCGATGACTACGTTGCACATTTCAAGATCAGTCCCATGTACCACAGAAATGTCGTGTCTGCTAGTTATGATCAGGGTTCTGAGAGATGGGTTGTGAAGACGATAAACAATGATGAGGGCTGTCGTGGTGAGGAGGAGGAATTTTTGGGAAGGTTTTTGGTGTTGGCTACTGGTGAAACAACAGATCCATATGTGCCAGAGATTGAAGGGTTGAGTAGTTTCAATGGGCAGGTTCTTCACTCGACCCGATTCAAATCCGGGGCTGAGTTCAAGAACAAGAATGTTTTGGTTGTCGGCTCTGGGAATTCTGGCCTGGAAATTGCTTTGGATCTGGCAAACCATGGTGCAAAGACTTCAATCATTGTTCGGAGCCCGATTCATTTTTTGTCAAGGAGGATGATGTACTGGGCCGTGGTTTTGGTGAGATATCTATCATTAAGCAAAGTGGAATCTTTGATGGTTTTGCTTAGCAAGCTGGTCTATGGTGACATGACCAAGTATGGGATAGGCAGGCCAAAAGAGGGCCCTTTCACTATGAAGATCAAGTACGGGAAGTACCCCATCATTGATGTCGGCACCTGTAGTAAGATCAAGTCCGGCGAGATTCAGGTTTTACCCGCAGAAATAGCTAGCATAAGAGGAAACGATGTGGAACTCAAAAATGGGAAGTCATACCAATTTGAGTCCATCGTTTTCTGCACCGGATTCAAGAGATCAACATGTTCGTGGCTCAAGGGGGATGATTATCTTTTGAAAGAAGATGGGATTCCAAGACAAAGTGCTCCAAACCATTGGAAAGGACAGAAAGGTTTGTATTGTGTTGGACTAGCAGGAAGAGGACTATACGGATCTGGTGTGGAGGCTCAAAACATAGCCAATGATATCAAGTCGTCTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00380 ko01100 map00380 map01100
Pfam Domains
Protein Families
FMO-like K_oxygenase Pyr_redox_3

Protein Analysis

381

Amino Acids

42.61

Weight (kDa)

8.7

Isoelectric Point (pI)

36.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FMO-like PF00743 6 - 319 1e-19 Flavin-binding monooxygenase-like
Pyr_redox_2 PF07992 6 - 209 1.8e-12 Pyridine nucleotide-disulphide oxidoreductase
Pyr_redox_3 PF13738 7 - 208 4.7e-23 Pyridine nucleotide-disulphide oxidoreductase
Lys_Orn_oxgnase PF13434 71 - 202 3.7e-13 L-lysine 6-monooxygenase/L-ornithine 5-monooxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 160, 466
AccB1I GGYRCC 1 cut(s) 821
AccII CGCG 1 cut(s) 50
AciI CCGC 2 cut(s) 32, 864
AclWI GGATC 3 cut(s) 425, 582, 1102
AcoI YGGCCR 1 cut(s) 54
AcsI RAATTY 2 cut(s) 386, 550
AcuI CTGAAG 1 cut(s) 224
AfaI GTAC 7 cut(s) 134, 222, 245, 287, 650, 794, 803
AfiI CCNNNNNNNGG 3 cut(s) 52, 747, 767
AflIII ACRYGT 2 cut(s) 189, 965
AgsI TTSAA 8 cut(s) 271, 452, 469, 499, 517, 603, 955, 1000
AjnI CCWGG 1 cut(s) 558
AjuI GAANNNNNNNTTGG 2 cut(s) 218, 250
AluBI AGCT 3 cut(s) 181, 721, 875
AluI AGCT 3 cut(s) 181, 721, 875
Alw21I GWGCWC 2 cut(s) 183, 1030
Alw26I GTCTC 2 cut(s) 68, 152
AlwI GGATC 3 cut(s) 425, 582, 1102
AoxI GGCC 7 cut(s) 34, 43, 54, 556, 654, 758, 769
ApaI GGGCCC 2 cut(s) 38, 773
ApoI RAATTY 2 cut(s) 386, 550
Asp700I GAANNNNTTC 1 cut(s) 233
AspS9I GGNCC 5 cut(s) 34, 35, 654, 769, 770
AsuC2I CCSGG 1 cut(s) 505
AsuHPI GGTGA 6 cut(s) 28, 149, 386, 431, 679, 743
AsuNHI GCTAGC 1 cut(s) 875
BaeGI GKGCMC 2 cut(s) 38, 773
BaeI ACNNNNGTAYC 2 cut(s) 204, 237
BanI GGYRCC 1 cut(s) 821
BanII GRGCYC 4 cut(s) 38, 183, 620, 773
BarI GAAGNNNNNNTAC 4 cut(s) 122, 154, 776, 808
BbsI GAAGAC 1 cut(s) 350
Bbv12I GWGCWC 2 cut(s) 183, 1030
BccI CCATC 6 cut(s) 46, 327, 697, 815, 941, 1001
BceAI ACGGC 1 cut(s) 641
BciT130I CCWGG 1 cut(s) 560
BclI TGATCA 1 cut(s) 316
BcnI CCSGG 1 cut(s) 505
BcoDI GTCTC 2 cut(s) 68, 152
BfaI CTAG 3 cut(s) 309, 876, 1073
BfmI CTRYAG 1 cut(s) 826
BfuAI ACCTGC 2 cut(s) 160, 466
BglI GCCNNNNNGGC 1 cut(s) 42
BlpI GCTNAGC 1 cut(s) 713
BmcAI AGTACT 2 cut(s) 134, 245
Bme1390I CCNGG 2 cut(s) 505, 560
BmgT120I GGNCC 5 cut(s) 34, 35, 654, 769, 770
BmiI GGNNCC 4 cut(s) 36, 617, 771, 823
BmrFI CCNGG 2 cut(s) 505, 560
BmrI ACTGGG 1 cut(s) 661
BmtI GCTAGC 1 cut(s) 879
BmuI ACTGGG 1 cut(s) 661
BpiI GAAGAC 1 cut(s) 350
Bpu1102I GCTNAGC 1 cut(s) 713
BpuEI CTTGAG 1 cut(s) 962
BpuMI CCSGG 1 cut(s) 505
BsaAI YACGTR 1 cut(s) 216
BsaBI GATNNNNATC 3 cut(s) 499, 676, 990
BsaJI CCNNGG 3 cut(s) 504, 586, 657
BsaWI WCCGGW 1 cut(s) 947
Bsc4I CCNNNNNNNGG 3 cut(s) 52, 747, 767
Bse118I RCCGGY 2 cut(s) 56, 69
Bse1I ACTGG 2 cut(s) 421, 656
Bse8I GATNNNNATC 3 cut(s) 499, 676, 990
BseBI CCWGG 1 cut(s) 560
BseDI CCNNGG 3 cut(s) 504, 586, 657
BseGI GGATG 2 cut(s) 648, 991
BseJI GATNNNNATC 3 cut(s) 499, 676, 990
BseLI CCNNNNNNNGG 3 cut(s) 52, 747, 767
BseMII CTCAG 3 cut(s) 79, 318, 501
BseNI ACTGG 2 cut(s) 421, 656
BseRI GAGGAG 3 cut(s) 108, 392, 395
BseSI GKGCMC 2 cut(s) 38, 773
BsgI GTGCAG 1 cut(s) 928
Bsh1236I CGCG 1 cut(s) 50
Bsh1285I CGRYCG 1 cut(s) 145
BshFI GGCC 7 cut(s) 36, 45, 56, 558, 656, 760, 771
BshNI GGYRCC 1 cut(s) 821
BsiEI CGRYCG 1 cut(s) 145
BsiHKAI GWGCWC 2 cut(s) 183, 1030
BsiSI CCGG 7 cut(s) 5, 42, 57, 70, 504, 843, 948
BslFI GGGAC 1 cut(s) 264
BslI CCNNNNNNNGG 3 cut(s) 52, 747, 767
BsmAI GTCTC 2 cut(s) 68, 152
BsmBI CGTCTC 1 cut(s) 152
BsmFI GGGAC 1 cut(s) 264
BsnI GGCC 7 cut(s) 36, 45, 56, 558, 656, 760, 771
Bsp120I GGGCCC 2 cut(s) 34, 769
Bsp1286I GDGCHC 5 cut(s) 38, 183, 620, 773, 1030
Bsp143I GATC 8 cut(s) 273, 316, 430, 574, 786, 834, 959, 1094
Bsp1720I GCTNAGC 1 cut(s) 713
Bsp19I CCATGG 1 cut(s) 586
Bsp68I TCGCGA 1 cut(s) 50
BspACI CCGC 2 cut(s) 32, 864
BspANI GGCC 7 cut(s) 36, 45, 56, 558, 656, 760, 771
BspCNI CTCAG 3 cut(s) 78, 319, 502
BspFNI CGCG 1 cut(s) 50
BspLI GGNNCC 4 cut(s) 36, 617, 771, 823
BspMI ACCTGC 2 cut(s) 160, 466
BspOI GCTAGC 1 cut(s) 879
BspPI GGATC 3 cut(s) 425, 582, 1102
BspT107I GGYRCC 1 cut(s) 821
BsrFI RCCGGY 2 cut(s) 56, 69
BsrI ACTGG 2 cut(s) 421, 656
BssAI RCCGGY 2 cut(s) 56, 69
BssECI CCNNGG 3 cut(s) 504, 586, 657
BssMI GATC 8 cut(s) 273, 316, 430, 574, 786, 834, 959, 1094
BssT1I CCWWGG 1 cut(s) 586
Bst2UI CCWGG 1 cut(s) 560
Bst4CI ACNGT 3 cut(s) 110, 146, 234
Bst6I CTCTTC 1 cut(s) 1075
BstBAI YACGTR 1 cut(s) 216
BstC8I GCNNGC 6 cut(s) 34, 58, 71, 719, 758, 877
BstDEI CTNAG 4 cut(s) 65, 327, 510, 713
BstDSI CCRYGG 2 cut(s) 586, 657
BstF5I GGATG 2 cut(s) 648, 991
BstFNI CGCG 1 cut(s) 50
BstKTI GATC 8 cut(s) 276, 319, 433, 577, 789, 837, 962, 1097
BstMAI GTCTC 2 cut(s) 68, 152
BstMBI GATC 8 cut(s) 273, 316, 430, 574, 786, 834, 959, 1094
BstMCI CGRYCG 1 cut(s) 145
BstMWI GCNNNNNNNGC 4 cut(s) 42, 66, 718, 872
BstNI CCWGG 1 cut(s) 560
BstNSI RCATGY 2 cut(s) 193, 969
BstSCI CCNGG 2 cut(s) 503, 558
BstSFI CTRYAG 1 cut(s) 826
BstSLI GKGCMC 2 cut(s) 38, 773
BstUI CGCG 1 cut(s) 50
BstV2I GAAGAC 1 cut(s) 350
BstX2I RGATCY 3 cut(s) 430, 574, 1094
BstXI CCANNNNNNTGG 1 cut(s) 1038
BstYI RGATCY 3 cut(s) 430, 574, 1094
BsuRI GGCC 7 cut(s) 36, 45, 56, 558, 656, 760, 771
BtgI CCRYGG 2 cut(s) 586, 657
BtgZI GCGATG 1 cut(s) 65
BtsCI GGATG 2 cut(s) 648, 991
BtsI GCAGTG 1 cut(s) 18
BtsIMutI CAGTG 1 cut(s) 18
BtuMI TCGCGA 1 cut(s) 50
BveI ACCTGC 2 cut(s) 160, 466
Cac8I GCNNGC 6 cut(s) 34, 58, 71, 719, 758, 877
Cfr10I RCCGGY 2 cut(s) 56, 69
Cfr13I GGNCC 5 cut(s) 34, 35, 654, 769, 770
Csp6I GTAC 7 cut(s) 133, 221, 244, 286, 649, 793, 802
CviAII CATG 5 cut(s) 190, 283, 587, 736, 966
CviQI GTAC 7 cut(s) 133, 221, 244, 286, 649, 793, 802
DdeI CTNAG 4 cut(s) 65, 327, 510, 713
DpnI GATC 8 cut(s) 275, 318, 432, 576, 788, 836, 961, 1096
DpnII GATC 8 cut(s) 273, 316, 430, 574, 786, 834, 959, 1094
EaeI YGGCCR 1 cut(s) 54
Eam1104I CTCTTC 1 cut(s) 1075
EarI CTCTTC 1 cut(s) 1075
Ecl136II GAGCTC 1 cut(s) 181
Eco130I CCWWGG 1 cut(s) 586
Eco24I GRGCYC 4 cut(s) 38, 183, 620, 773
Eco32I GATATC 2 cut(s) 674, 1129
Eco53kI GAGCTC 1 cut(s) 181
Eco57I CTGAAG 1 cut(s) 224
EcoICRI GAGCTC 1 cut(s) 181
EcoO109I RGGNCCY 2 cut(s) 769, 770
EcoRI GAATTC 1 cut(s) 550
EcoRII CCWGG 1 cut(s) 558
EcoRV GATATC 2 cut(s) 674, 1129
EcoT14I CCWWGG 1 cut(s) 586
EcoT38I GRGCYC 4 cut(s) 38, 183, 620, 773
ErhI CCWWGG 1 cut(s) 586
Esp3I CGTCTC 1 cut(s) 152
FaeI CATG 5 cut(s) 193, 286, 590, 739, 969
FaqI GGGAC 1 cut(s) 264
FatI CATG 5 cut(s) 189, 282, 586, 735, 965
FauI CCCGC 2 cut(s) 25, 871
FauNDI CATATG 1 cut(s) 436
FbaI TGATCA 1 cut(s) 316
FokI GGATG 2 cut(s) 655, 998
FriOI GRGCYC 4 cut(s) 38, 183, 620, 773
FspBI CTAG 3 cut(s) 309, 876, 1073
HaeIII GGCC 7 cut(s) 36, 45, 56, 558, 656, 760, 771
HapII CCGG 7 cut(s) 5, 42, 57, 70, 504, 843, 948
Hin1II CATG 5 cut(s) 193, 286, 590, 739, 969
HinfI GANTC 7 cut(s) 495, 622, 695, 850, 929, 951, 1012
HpaII CCGG 7 cut(s) 5, 42, 57, 70, 504, 843, 948
HphI GGTGA 6 cut(s) 28, 149, 386, 431, 679, 743
Hpy188I TCNGA 2 cut(s) 328, 615
Hpy188III TCNNGA 5 cut(s) 49, 95, 271, 517, 955
HpyAV CCTTC 5 cut(s) 164, 170, 248, 390, 446
HpyCH4III ACNGT 3 cut(s) 110, 146, 234
HpyCH4IV ACGT 2 cut(s) 215, 258
HpyCH4V TGCA 3 cut(s) 263, 593, 945
HpyF10VI GCNNNNNNNGC 4 cut(s) 42, 66, 718, 872
HpyF3I CTNAG 4 cut(s) 65, 327, 510, 713
HpySE526I ACGT 2 cut(s) 215, 258
Hsp92II CATG 5 cut(s) 193, 286, 590, 739, 969
KroI GCCGGC 2 cut(s) 56, 69
KroNI GCCGGC 2 cut(s) 58, 71
Ksp22I TGATCA 1 cut(s) 316
Kzo9I GATC 8 cut(s) 273, 316, 430, 574, 786, 834, 959, 1094
LmnI GCTCC 4 cut(s) 121, 186, 615, 1033
MaeI CTAG 3 cut(s) 309, 876, 1073
MaeII ACGT 2 cut(s) 215, 258
MaeIII GTNAC 1 cut(s) 731
MalI GATC 8 cut(s) 275, 318, 432, 576, 788, 836, 961, 1096
MboI GATC 8 cut(s) 273, 316, 430, 574, 786, 834, 959, 1094
MboII GAAGA 6 cut(s) 139, 355, 473, 796, 1016, 1092
MflI RGATCY 3 cut(s) 430, 574, 1094
MhlI GDGCHC 5 cut(s) 38, 183, 620, 773, 1030
MluCI AATT 4 cut(s) 386, 550, 564, 923
MlyI GAGTC 1 cut(s) 938
MmeI TCCRAC 1 cut(s) 1048
MroNI GCCGGC 2 cut(s) 56, 69
MroXI GAANNNNTTC 1 cut(s) 233
MseI TTAA 1 cut(s) 683
MslI CAYNNNNRTG 2 cut(s) 294, 970
MspI CCGG 7 cut(s) 5, 42, 57, 70, 504, 843, 948
MspR9I CCNGG 2 cut(s) 505, 560
MvaI CCWGG 1 cut(s) 560
MvnI CGCG 1 cut(s) 50
MwoI GCNNNNNNNGC 4 cut(s) 42, 66, 718, 872
NaeI GCCGGC 2 cut(s) 58, 71
NciI CCSGG 1 cut(s) 505
NcoI CCATGG 1 cut(s) 586
NdeI CATATG 1 cut(s) 436
NdeII GATC 8 cut(s) 273, 316, 430, 574, 786, 834, 959, 1094
NgoMIV GCCGGC 2 cut(s) 56, 69
NheI GCTAGC 1 cut(s) 875
NlaIII CATG 5 cut(s) 193, 286, 590, 739, 969
NlaIV GGNNCC 4 cut(s) 36, 617, 771, 823
NmuCI GTSAC 1 cut(s) 731
NruI TCGCGA 1 cut(s) 50
NspI RCATGY 2 cut(s) 193, 969
PciI ACATGT 2 cut(s) 189, 965
PcsI WCGNNNNNNNCGW 1 cut(s) 255
PdiI GCCGGC 2 cut(s) 58, 71
PdmI GAANNNNTTC 1 cut(s) 233
PfeI GAWTC 6 cut(s) 495, 622, 695, 850, 951, 1012
PleI GAGTC 1 cut(s) 937
PpsI GAGTC 1 cut(s) 937
Ppu21I YACGTR 1 cut(s) 216
PscI ACATGT 2 cut(s) 189, 965
Psp124BI GAGCTC 1 cut(s) 183
Psp6I CCWGG 1 cut(s) 558
PspGI CCWGG 1 cut(s) 558
PspN4I GGNNCC 4 cut(s) 36, 617, 771, 823
PspOMI GGGCCC 2 cut(s) 34, 769
PspPI GGNCC 5 cut(s) 34, 35, 654, 769, 770
PsuI RGATCY 3 cut(s) 430, 574, 1094
RruI TCGCGA 1 cut(s) 50
RsaI GTAC 7 cut(s) 134, 222, 245, 287, 650, 794, 803
RsaNI GTAC 7 cut(s) 133, 221, 244, 286, 649, 793, 802
RseI CAYNNNNRTG 2 cut(s) 294, 970
SacI GAGCTC 1 cut(s) 183
SaqAI TTAA 1 cut(s) 683
Sau3AI GATC 8 cut(s) 273, 316, 430, 574, 786, 834, 959, 1094
Sau96I GGNCC 5 cut(s) 34, 35, 654, 769, 770
ScaI AGTACT 2 cut(s) 134, 245
SchI GAGTC 1 cut(s) 938
ScrFI CCNGG 2 cut(s) 505, 560
SduI GDGCHC 5 cut(s) 38, 183, 620, 773, 1030
SfcI CTRYAG 1 cut(s) 826
SfiI GGCCNNNNNGGCC 1 cut(s) 42
SmiMI CAYNNNNRTG 2 cut(s) 294, 970
SmlI CTYRAG 1 cut(s) 977
SmoI CTYRAG 1 cut(s) 977
Sse9I AATT 4 cut(s) 386, 550, 564, 923
SsiI CCGC 2 cut(s) 32, 864
SspMI CTAG 3 cut(s) 309, 876, 1073
SstI GAGCTC 1 cut(s) 183
StyD4I CCNGG 2 cut(s) 503, 558
StyI CCWWGG 1 cut(s) 586
TaaI ACNGT 3 cut(s) 110, 146, 234
TaiI ACGT 2 cut(s) 218, 261
TaqI TCGA 4 cut(s) 75, 96, 249, 488
TasI AATT 4 cut(s) 386, 550, 564, 923
TatI WGTACW 3 cut(s) 132, 243, 648
TfiI GAWTC 6 cut(s) 495, 622, 695, 850, 951, 1012
Tru1I TTAA 1 cut(s) 683
Tru9I TTAA 1 cut(s) 683
TscAI CASTG 1 cut(s) 18
TseFI GTSAC 1 cut(s) 731
Tsp45I GTSAC 1 cut(s) 731
TspDTI ATGAA 3 cut(s) 152, 614, 797
TspGWI ACGGA 1 cut(s) 1107
TspRI CASTG 1 cut(s) 18
XapI RAATTY 2 cut(s) 386, 550
XceI RCATGY 2 cut(s) 193, 969
XmnI GAANNNNTTC 1 cut(s) 233
XspI CTAG 3 cut(s) 309, 876, 1073
ZrmI AGTACT 2 cut(s) 134, 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.