pycom02g16610

microtubule binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0001938
Physical Location & Seq
Forward (+)
13826033 .. 13831200
5168 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2448 bp
ATGGAAACGACGCCGTCTATAATCAAAACCGTACTATGTGCGATTCGCATTTCTCAATATAACGCTCCCCTCCTCTTTCCTTCCCTCGCACGCAAAACTCGCGCTCCCGACAAAAAAATCCAAGCGTTACGCAGAGAGAGATTCACACTTCACTTACACTCGCTAGGGTTTCTGAATTCGCGCTTGTCATTCTCTGATCGATCATCTGGAAAGCTCCGCAGCGATAAGGTCCATTTTCTTCCTTATGCTTTGTTTGTTTCTGGGGAAAATATGCCCGGAAAATGTAGCAATTCTTCTCACAAGAATCTTACGGAGTTTGTTGCCATTTACAGTGTTTTAGGTACTTCACCATGTTCAGTCAGCAAGGCAGTAATCATTTTGCGCATATGGAAACAAAGTTTGAATCTTTGCTGCAGGAACTGCAGGGATGCAATGTTTCTAAAAATCGAACAAGAGTGTATGGAGGTATCTAGGAGAAAAGTTGACGAGGCGAAAAATTGTAAAGCTCAACTTCTGCAAGAAATTGCTAATCACAAAGTAGAGCTTGTAGACATATGTGCTGCATTGGGAGAGCAGCCACAACACCTTGATCAAAAGGCCAGTGGAAGTTTGAAGAAAGAGCTTGAAACCATTATCCAACAACTAGAGGACATGAAGATGCAGAAAATGGAAAGAAGGAATGAATTCTTTATTGTCTTAGATCAATTACAGAAAATTTCGAATGCAATAGGCAAATGCATGACAGACAGTCTGTACAAAATGGTAGTGGAAGAGACTGACTTGTCCTTGAAGAGACTTGAAGAATTGCGTAGACGGCTGCTTGAATATCAAGATGAGAAGAGAAGCCGTTTGAATCTGATAATGGACTACCTAAACATGATAAGCTCACTCTGCTTAGTTCTCGGTATGGATTTTAAGCAAACAATTCATGAAATCCACCCCACTTTGATTGATTCTGAGGGAGCAAAGGACATAAGCAACAATACAATTGATAGCTTGGATAACTCGTTACAAAGTTTGAGAAAGCTCAAGAATCAGAGGTGGCAGAGGCTCCAAGATCATGCAAGTGCTCTGTTTGAACTGTGGAATCTGATGGATACACCAATAGAGGAACAAAATAAATTTCAGAATGTAACTAGTCATATAACTGCTTTAGAATCTGAAATCACTGAGCCTAACATGCTGTCTACAGACGTTCTAAATCATGTTGAGGCTGAAGTGTCAAGGTTGCAGCAGCTCAAATCAAGTAAGCTAAAAGAGATTAGTCAGAAAAAGAAGTTGGAACTGGAGGAGATATGCAAGCGGTCTCATATGGTTACAGAAACATTTAGTGCAATGGAACACTCAATTGAAGCAGTAGAGTCTGGAACTGCGGACACTGCATGCCTGTTGGAACAGATTGATCTCCAAATCACAAGGGCGAAAGAGGAAGCTTCAAGCAGGAAAGAGATACTAGAAAAGGTTGAGAAGTGGTTGGCTGCGCGTCAAGAAGAATGTTGGCTAGAGGAGTACAACAGGGATGAAAACCGCTATAATGCAGGGAGAGGAGCCCATCTTACATTGAAACGTGCAGAGAAAGCTCGTGCTTTGGTAAACAAAATTCCAGGGATGTTGGAAGCATTGACTTCAAAAGCCACAGCTTGGGAAAGAGAAAGAGGAACTGAATTCTTGTATGATGGTGGCAAACTTCTTTCAATGCTCAATCAGTACAGCACCTTAAGGCAGGAGAACGAGCAAGAACAGCAGAGACAGAGAGATATGAAAAAATTTCAGGGGCAGGTGCTAGTTGAGCAGGAAGCATTTTATGGGTCAAAACGTAGTCCATCCAAGAGTGGAAAGAAGGCTTCCAATAGAAAAATGTCCCTAGGTGGAGCATTAATCCGAAGCCAAAAGAATGAAAAAGTTACTCCGCCTTTACACCCTACAAAGAAGGGTGACTTTCTCCATCGAAGTAGCTCTCGCCAGCAATACAGTGGAGTGGAAGCTCCATCCTTTGGAAGGAGAACCTCAGAGATCTATGGTCATTCAGTGAAGAAGCGCCCAACCAGTGCTGCAAAGGCTCTTGAAATGGAATCCCCATTGATCCGGAAACCGCTCTCTCCTGTCTATTCTGAAGTGTCATCTATGGCCAACATTGCAAATTACTTTGAAGGTCAGAAGAGACAACAGGAGACAAACATCCAAGCAAGCCATCTTTTAAGTGAAAAGCCAATCATGACTCCTTCCAAGCCGAAAAATGCGGGTGATGATGAAGAGAATAGAACCCCAAAGACGAAGCCAATTCCAGTACCTCCAACTCCTTCAACAATATCAATTCATATGCTGACGGCCATGACACCAGCCACACCTTTCACTTCTGGTGATTACAGGGTTGAGAAAGCTGATCAACGACTTAAGTACTCGTTCGAGGAGGTGAGAGCAATTAGATATGAACTTGGTAGCCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MAP65_ASE1

Protein Analysis

816

Amino Acids

93.1

Weight (kDa)

9.04

Isoelectric Point (pI)

58.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAP65_ASE1 PF03999 129 - 611 4.8e-94 Microtubule associated protein (MAP65/ASE1 family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1768
AasI GACNNNNNNGTC 1 cut(s) 781
Acc16I TGCGCA 1 cut(s) 383
Acc36I ACCTGC 1 cut(s) 1768
AccB7I CCANNNNNTGG 2 cut(s) 1641, 1994
AccBSI CCGCTC 1 cut(s) 2095
AccI GTMKAC 3 cut(s) 549, 811, 1187
AccII CGCG 3 cut(s) 102, 181, 1483
AccIII TCCGGA 1 cut(s) 2085
AciI CCGC 7 cut(s) 217, 1303, 1373, 1528, 1910, 2093, 2240
AclWI GGATC 1 cut(s) 2077
AcoI YGGCCR 2 cut(s) 2127, 2328
AcsI RAATTY 7 cut(s) 175, 683, 714, 1121, 1599, 1664, 1766
AcuI CTGAAG 2 cut(s) 1236, 2133
AcyI GRCGYC 1 cut(s) 11
AfaI GTAC 7 cut(s) 33, 343, 755, 1511, 1709, 2289, 2399
AfiI CCNNNNNNNGG 3 cut(s) 1641, 1994, 1998
AflII CTTAAG 2 cut(s) 1717, 2393
AhdI GACNNNNNGTC 1 cut(s) 747
AhlI ACTAGT 1 cut(s) 1136
AjnI CCWGG 1 cut(s) 1603
AjuI GAANNNNNNNTTGG 2 cut(s) 1262, 1294
Alw21I GWGCWC 1 cut(s) 1072
Alw26I GTCTC 6 cut(s) 767, 787, 1311, 1741, 2155, 2165
AlwI GGATC 1 cut(s) 2077
AlwNI CAGNNNCTG 1 cut(s) 420
Aor13HI TCCGGA 1 cut(s) 2085
AoxI GGCC 3 cut(s) 597, 2127, 2328
ApeKI GCWGC 9 cut(s) 219, 411, 560, 574, 817, 1231, 1234, 1478, 2051
ApoI RAATTY 7 cut(s) 175, 683, 714, 1121, 1599, 1664, 1766
AseI ATTAAT 1 cut(s) 1877
Asp700I GAANNNNTTC 1 cut(s) 683
AspA2I CCTAGG 1 cut(s) 1864
AspLEI GCGC 5 cut(s) 104, 183, 384, 1483, 2040
AspS9I GGNCC 1 cut(s) 229
AsuC2I CCSGG 1 cut(s) 276
AsuHPI GGTGA 5 cut(s) 339, 1946, 2255, 2372, 2425
AsuII TTCGAA 1 cut(s) 719
AvaII GGWCC 1 cut(s) 229
AvrII CCTAGG 1 cut(s) 1864
BalI TGGCCA 1 cut(s) 2129
BanII GRGCYC 1 cut(s) 1552
BarI GAAGNNNNNNTAC 2 cut(s) 277, 309
BauI CACGAG 1 cut(s) 1581
Bbv12I GWGCWC 1 cut(s) 1072
BbvI GCAGC 9 cut(s) 231, 398, 547, 586, 804, 1243, 1246, 1465, 2038
BccI CCATC 7 cut(s) 1087, 1560, 1670, 1831, 1953, 1996, 2199
BceAI ACGGC 3 cut(s) 830, 831, 2343
BciT130I CCWGG 1 cut(s) 1605
BciVI GTATCC 1 cut(s) 1090
BclI TGATCA 2 cut(s) 589, 2383
BcnI CCSGG 1 cut(s) 276
BcoDI GTCTC 6 cut(s) 767, 787, 1311, 1741, 2155, 2165
BcuI ACTAGT 1 cut(s) 1136
BfaI CTAG 8 cut(s) 164, 471, 644, 1137, 1454, 1502, 1784, 1865
BfmI CTRYAG 3 cut(s) 412, 421, 1188
BfoI RGCGCY 1 cut(s) 2041
BfrI CTTAAG 2 cut(s) 1717, 2393
BfuAI ACCTGC 1 cut(s) 1768
BfuI GTATCC 1 cut(s) 1090
BglII AGATCT 1 cut(s) 2013
BisI GCNGC 9 cut(s) 220, 412, 561, 575, 818, 1232, 1235, 1479, 2052
BlnI CCTAGG 1 cut(s) 1864
BlsI GCNGC 9 cut(s) 221, 413, 562, 576, 819, 1233, 1236, 1480, 2053
BmcAI AGTACT 1 cut(s) 2399
Bme1390I CCNGG 2 cut(s) 276, 1605
Bme18I GGWCC 1 cut(s) 229
BmeRI GACNNNNNGTC 1 cut(s) 747
BmgT120I GGNCC 1 cut(s) 229
BmiI GGNNCC 2 cut(s) 1052, 1549
BmrFI CCNGG 2 cut(s) 276, 1605
BmsI GCATC 2 cut(s) 418, 648
BpmI CTGGAG 1 cut(s) 1307
Bpu14I TTCGAA 1 cut(s) 719
BpuEI CTTGAG 1 cut(s) 1013
BpuMI CCSGG 1 cut(s) 276
Bsa29I ATCGAT 1 cut(s) 199
BsaHI GRCGYC 1 cut(s) 11
BsaI GGTCTC 1 cut(s) 1311
BsaJI CCNNGG 2 cut(s) 1604, 1864
BsaWI WCCGGW 1 cut(s) 2085
BsaXI ACNNNNNCTCC 2 cut(s) 88, 118
Bsc4I CCNNNNNNNGG 3 cut(s) 1641, 1994, 1998
Bse1I ACTGG 5 cut(s) 600, 1290, 2046, 2285, 2443
Bse3DI GCAATG 3 cut(s) 438, 1341, 2133
BseAI TCCGGA 1 cut(s) 2085
BseBI CCWGG 1 cut(s) 1605
BseCI ATCGAT 1 cut(s) 199
BseDI CCNNGG 2 cut(s) 1604, 1864
BseGI GGATG 6 cut(s) 433, 1525, 1614, 1823, 1988, 2178
BseLI CCNNNNNNNGG 3 cut(s) 1641, 1994, 1998
BseMI GCAATG 3 cut(s) 438, 1341, 2133
BseMII CTCAG 3 cut(s) 948, 1161, 2022
BseNI ACTGG 5 cut(s) 600, 1290, 2046, 2285, 2443
BseRI GAGGAG 5 cut(s) 62, 1304, 1520, 1560, 2423
BseXI GCAGC 9 cut(s) 231, 398, 547, 586, 804, 1243, 1246, 1465, 2038
BsgI GTGCAG 1 cut(s) 1590
Bsh1236I CGCG 3 cut(s) 102, 181, 1483
BshFI GGCC 3 cut(s) 599, 2129, 2330
BshVI ATCGAT 1 cut(s) 199
BsiHKAI GWGCWC 1 cut(s) 1072
BsiSI CCGG 2 cut(s) 276, 2086
BslFI GGGAC 1 cut(s) 1846
BslI CCNNNNNNNGG 3 cut(s) 1641, 1994, 1998
BsmAI GTCTC 6 cut(s) 767, 787, 1311, 1741, 2155, 2165
BsmFI GGGAC 1 cut(s) 1846
BsmI GAATGC 1 cut(s) 727
BsnI GGCC 3 cut(s) 599, 2129, 2330
Bso31I GGTCTC 1 cut(s) 1311
Bsp119I TTCGAA 1 cut(s) 719
Bsp1286I GDGCHC 2 cut(s) 1072, 1552
Bsp13I TCCGGA 1 cut(s) 2085
Bsp1407I TGTACA 1 cut(s) 753
Bsp143I GATC 9 cut(s) 196, 200, 589, 700, 1057, 1402, 2013, 2082, 2383
BspACI CCGC 7 cut(s) 217, 1303, 1373, 1528, 1910, 2093, 2240
BspANI GGCC 3 cut(s) 599, 2129, 2330
BspCNI CTCAG 3 cut(s) 949, 1162, 2021
BspDI ATCGAT 1 cut(s) 199
BspEI TCCGGA 1 cut(s) 2085
BspFNI CGCG 3 cut(s) 102, 181, 1483
BspHI TCATGA 2 cut(s) 928, 2214
BspLI GGNNCC 2 cut(s) 1052, 1549
BspMAI CTGCAG 2 cut(s) 416, 425
BspMI ACCTGC 1 cut(s) 1768
BspPI GGATC 1 cut(s) 2077
BspT104I TTCGAA 1 cut(s) 719
BspTI CTTAAG 2 cut(s) 1717, 2393
BspTNI GGTCTC 1 cut(s) 1311
BsrBI CCGCTC 1 cut(s) 2095
BsrDI GCAATG 3 cut(s) 438, 1341, 2133
BsrGI TGTACA 1 cut(s) 753
BsrI ACTGG 5 cut(s) 600, 1290, 2046, 2285, 2443
BssECI CCNNGG 2 cut(s) 1604, 1864
BssMI GATC 9 cut(s) 196, 200, 589, 700, 1057, 1402, 2013, 2082, 2383
BssNI GRCGYC 1 cut(s) 11
BssSI CACGAG 1 cut(s) 1581
BssT1I CCWWGG 1 cut(s) 1864
Bst2BI CACGAG 1 cut(s) 1581
Bst2UI CCWGG 1 cut(s) 1605
Bst4CI ACNGT 5 cut(s) 31, 332, 749, 1083, 1973
Bst6I CTCTTC 5 cut(s) 765, 785, 833, 2153, 2247
BstACI GRCGYC 1 cut(s) 11
BstAFI CTTAAG 2 cut(s) 1717, 2393
BstAPI GCANNNNNTGC 1 cut(s) 420
BstAUI TGTACA 1 cut(s) 753
BstBI TTCGAA 1 cut(s) 719
BstC8I GCNNGC 5 cut(s) 91, 1301, 1384, 1964, 2188
BstDEI CTNAG 5 cut(s) 697, 895, 957, 1170, 2008
BstENI CCTNNNNNAGG 1 cut(s) 1996
BstF5I GGATG 6 cut(s) 433, 1525, 1614, 1823, 1988, 2178
BstFNI CGCG 3 cut(s) 102, 181, 1483
BstH2I RGCGCY 1 cut(s) 2041
BstHHI GCGC 5 cut(s) 104, 183, 384, 1483, 2040
BstKTI GATC 9 cut(s) 199, 203, 592, 703, 1060, 1405, 2016, 2085, 2386
BstMAI GTCTC 6 cut(s) 767, 787, 1311, 1741, 2155, 2165
BstMBI GATC 9 cut(s) 196, 200, 589, 700, 1057, 1402, 2013, 2082, 2383
BstNI CCWGG 1 cut(s) 1605
BstNSI RCATGY 2 cut(s) 1183, 1386
BstSCI CCNGG 2 cut(s) 274, 1603
BstSFI CTRYAG 3 cut(s) 412, 421, 1188
BstUI CGCG 3 cut(s) 102, 181, 1483
BstV1I GCAGC 9 cut(s) 231, 398, 547, 586, 804, 1243, 1246, 1465, 2038
BstX2I RGATCY 1 cut(s) 2013
BstYI RGATCY 1 cut(s) 2013
Bsu15I ATCGAT 1 cut(s) 199
BsuI GTATCC 1 cut(s) 1090
BsuRI GGCC 3 cut(s) 599, 2129, 2330
BsuTUI ATCGAT 1 cut(s) 199
BtsCI GGATG 6 cut(s) 433, 1525, 1614, 1823, 1988, 2178
BtsI GCAGTG 1 cut(s) 1377
BtsIMutI CAGTG 7 cut(s) 337, 607, 1167, 1377, 1978, 2034, 2053
BveI ACCTGC 1 cut(s) 1768
Cac8I GCNNGC 5 cut(s) 91, 1301, 1384, 1964, 2188
CaiI CAGNNNCTG 1 cut(s) 420
CciI TCATGA 2 cut(s) 928, 2214
CfoI GCGC 5 cut(s) 104, 183, 384, 1483, 2040
Cfr13I GGNCC 1 cut(s) 229
ClaI ATCGAT 1 cut(s) 199
CseI GACGC 2 cut(s) 19, 1472
Csp6I GTAC 7 cut(s) 32, 342, 754, 1510, 1708, 2288, 2398
CspCI CAANNNNNGTGG 2 cut(s) 931, 966
CviQI GTAC 7 cut(s) 32, 342, 754, 1510, 1708, 2288, 2398
DdeI CTNAG 5 cut(s) 697, 895, 957, 1170, 2008
DpnI GATC 9 cut(s) 198, 202, 591, 702, 1059, 1404, 2015, 2084, 2385
DpnII GATC 9 cut(s) 196, 200, 589, 700, 1057, 1402, 2013, 2082, 2383
DrdI GACNNNNNNGTC 1 cut(s) 781
DriI GACNNNNNGTC 1 cut(s) 747
DseDI GACNNNNNNGTC 1 cut(s) 781
EaeI YGGCCR 2 cut(s) 2127, 2328
Eam1104I CTCTTC 5 cut(s) 765, 785, 833, 2153, 2247
Eam1105I GACNNNNNGTC 1 cut(s) 747
EarI CTCTTC 5 cut(s) 765, 785, 833, 2153, 2247
EciI GGCGGA 1 cut(s) 1899
Eco130I CCWWGG 1 cut(s) 1864
Eco24I GRGCYC 1 cut(s) 1552
Eco31I GGTCTC 1 cut(s) 1311
Eco47I GGWCC 1 cut(s) 229
Eco57I CTGAAG 2 cut(s) 1236, 2133
EcoNI CCTNNNNNAGG 1 cut(s) 1996
EcoRI GAATTC 3 cut(s) 175, 683, 1664
EcoRII CCWGG 1 cut(s) 1603
EcoT14I CCWWGG 1 cut(s) 1864
EcoT22I ATGCAT 1 cut(s) 740
EcoT38I GRGCYC 1 cut(s) 1552
ErhI CCWWGG 1 cut(s) 1864
FalI AAGNNNNNCTT 6 cut(s) 495, 527, 528, 560, 1922, 1954
FaqI GGGAC 1 cut(s) 1846
FauI CCCGC 1 cut(s) 2233
FauNDI CATATG 4 cut(s) 386, 554, 1311, 2319
FbaI TGATCA 2 cut(s) 589, 2383
FblI GTMKAC 3 cut(s) 549, 811, 1187
Fnu4HI GCNGC 9 cut(s) 220, 412, 561, 575, 818, 1232, 1235, 1479, 2052
FokI GGATG 6 cut(s) 440, 1532, 1621, 1810, 1975, 2165
FriOI GRGCYC 1 cut(s) 1552
Fsp4HI GCNGC 9 cut(s) 220, 412, 561, 575, 818, 1232, 1235, 1479, 2052
FspBI CTAG 8 cut(s) 164, 471, 644, 1137, 1454, 1502, 1784, 1865
FspI TGCGCA 1 cut(s) 383
GlaI GCGC 5 cut(s) 103, 182, 383, 1482, 2039
GluI GCNGC 9 cut(s) 220, 412, 561, 575, 818, 1232, 1235, 1479, 2052
GsuI CTGGAG 1 cut(s) 1307
HaeII RGCGCY 1 cut(s) 2041
HaeIII GGCC 3 cut(s) 599, 2129, 2330
HapII CCGG 2 cut(s) 276, 2086
HgaI GACGC 2 cut(s) 19, 1472
HhaI GCGC 5 cut(s) 104, 183, 384, 1483, 2040
Hin1I GRCGYC 1 cut(s) 11
Hin6I GCGC 5 cut(s) 102, 181, 382, 1481, 2038
HinP1I GCGC 5 cut(s) 102, 181, 382, 1481, 2038
HincII GTYRAC 1 cut(s) 484
HindII GTYRAC 1 cut(s) 484
HindIII AAGCTT 1 cut(s) 1431
HpaII CCGG 2 cut(s) 276, 2086
HphI GGTGA 5 cut(s) 339, 1946, 2255, 2372, 2425
Hpy166II GTNNAC 5 cut(s) 484, 550, 812, 1188, 1594
Hpy8I GTNNAC 5 cut(s) 484, 550, 812, 1188, 1594
Hpy99I CGWCG 1 cut(s) 13
HpyAV CCTTC 8 cut(s) 90, 669, 1834, 1924, 1992, 2144, 2232, 2310
HpyCH4III ACNGT 5 cut(s) 31, 332, 749, 1083, 1973
HpyCH4IV ACGT 3 cut(s) 1194, 1567, 1816
HpyF3I CTNAG 5 cut(s) 697, 895, 957, 1170, 2008
HpySE526I ACGT 3 cut(s) 1194, 1567, 1816
Hsp92I GRCGYC 1 cut(s) 11
HspAI GCGC 5 cut(s) 102, 181, 382, 1481, 2038
Kpn2I TCCGGA 1 cut(s) 2085
Ksp22I TGATCA 2 cut(s) 589, 2383
Kzo9I GATC 9 cut(s) 196, 200, 589, 700, 1057, 1402, 2013, 2082, 2383
LmnI GCTCC 8 cut(s) 70, 109, 219, 962, 1056, 1547, 1871, 1990
Lsp1109I GCAGC 9 cut(s) 231, 398, 547, 586, 804, 1243, 1246, 1465, 2038
LweI GCATC 2 cut(s) 418, 648
MaeI CTAG 8 cut(s) 164, 471, 644, 1137, 1454, 1502, 1784, 1865
MaeII ACGT 3 cut(s) 1194, 1567, 1816
MaeIII GTNAC 6 cut(s) 126, 1008, 1132, 1315, 1903, 1934
MalI GATC 9 cut(s) 198, 202, 591, 702, 1059, 1404, 2015, 2084, 2385
MbiI CCGCTC 1 cut(s) 2095
MboI GATC 9 cut(s) 196, 200, 589, 700, 1057, 1402, 2013, 2082, 2383
MfeI CAATTG 2 cut(s) 987, 1347
MflI RGATCY 1 cut(s) 2013
MhlI GDGCHC 2 cut(s) 1072, 1552
MlsI TGGCCA 1 cut(s) 2129
MluNI TGGCCA 1 cut(s) 2129
MlyI GAGTC 2 cut(s) 1370, 2212
MmeI TCCRAC 5 cut(s) 661, 1260, 1371, 1593, 2318
Mox20I TGGCCA 1 cut(s) 2129
Mph1103I ATGCAT 1 cut(s) 740
MroI TCCGGA 1 cut(s) 2085
MroXI GAANNNNTTC 1 cut(s) 683
MscI TGGCCA 1 cut(s) 2129
MseI TTAA 5 cut(s) 915, 1718, 1877, 2198, 2394
MslI CAYNNNNRTG 2 cut(s) 656, 1065
Msp20I TGGCCA 1 cut(s) 2129
MspCI CTTAAG 2 cut(s) 1717, 2393
MspI CCGG 2 cut(s) 276, 2086
MspR9I CCNGG 2 cut(s) 276, 1605
MunI CAATTG 2 cut(s) 987, 1347
Mva1269I GAATGC 1 cut(s) 727
MvaI CCWGG 1 cut(s) 1605
MvnI CGCG 3 cut(s) 102, 181, 1483
NciI CCSGG 1 cut(s) 276
NdeI CATATG 4 cut(s) 386, 554, 1311, 2319
NdeII GATC 9 cut(s) 196, 200, 589, 700, 1057, 1402, 2013, 2082, 2383
NlaIV GGNNCC 2 cut(s) 1052, 1549
NmuCI GTSAC 1 cut(s) 1934
NsbI TGCGCA 1 cut(s) 383
NsiI ATGCAT 1 cut(s) 740
NspI RCATGY 2 cut(s) 1183, 1386
NspV TTCGAA 1 cut(s) 719
PaeI GCATGC 1 cut(s) 1386
PagI TCATGA 2 cut(s) 928, 2214
PaqCI CACCTGC 1 cut(s) 1768
PctI GAATGC 1 cut(s) 727
PdmI GAANNNNTTC 1 cut(s) 683
PflFI GACNNNGTC 1 cut(s) 13
PflMI CCANNNNNTGG 2 cut(s) 1641, 1994
PkrI GCNGC 9 cut(s) 221, 413, 562, 576, 819, 1233, 1236, 1480, 2053
PleI GAGTC 2 cut(s) 1369, 2212
PpsI GAGTC 2 cut(s) 1369, 2212
PshBI ATTAAT 1 cut(s) 1877
Psp6I CCWGG 1 cut(s) 1603
PspGI CCWGG 1 cut(s) 1603
PspN4I GGNNCC 2 cut(s) 1052, 1549
PspPI GGNCC 1 cut(s) 229
PstI CTGCAG 2 cut(s) 416, 425
PstNI CAGNNNCTG 1 cut(s) 420
PsuI RGATCY 1 cut(s) 2013
PsyI GACNNNGTC 1 cut(s) 13
RsaI GTAC 7 cut(s) 33, 343, 755, 1511, 1709, 2289, 2399
RsaNI GTAC 7 cut(s) 32, 342, 754, 1510, 1708, 2288, 2398
RseI CAYNNNNRTG 2 cut(s) 656, 1065
SaqAI TTAA 5 cut(s) 915, 1718, 1877, 2198, 2394
SatI GCNGC 9 cut(s) 220, 412, 561, 575, 818, 1232, 1235, 1479, 2052
Sau3AI GATC 9 cut(s) 196, 200, 589, 700, 1057, 1402, 2013, 2082, 2383
Sau96I GGNCC 1 cut(s) 229
ScaI AGTACT 1 cut(s) 2399
SchI GAGTC 2 cut(s) 1370, 2212
ScrFI CCNGG 2 cut(s) 276, 1605
SduI GDGCHC 2 cut(s) 1072, 1552
SfaNI GCATC 2 cut(s) 418, 648
SfcI CTRYAG 3 cut(s) 412, 421, 1188
SfuI TTCGAA 1 cut(s) 719
SinI GGWCC 1 cut(s) 229
SmiMI CAYNNNNRTG 2 cut(s) 656, 1065
SmlI CTYRAG 3 cut(s) 1028, 1717, 2393
SmoI CTYRAG 3 cut(s) 1028, 1717, 2393
SpeI ACTAGT 1 cut(s) 1136
SphI GCATGC 1 cut(s) 1386
SsiI CCGC 7 cut(s) 217, 1303, 1373, 1528, 1910, 2093, 2240
SspMI CTAG 8 cut(s) 164, 471, 644, 1137, 1454, 1502, 1784, 1865
StyD4I CCNGG 2 cut(s) 274, 1603
StyI CCWWGG 1 cut(s) 1864
TaaI ACNGT 5 cut(s) 31, 332, 749, 1083, 1973
TaiI ACGT 3 cut(s) 1197, 1570, 1819
TaqI TCGA 5 cut(s) 199, 447, 719, 1948, 2406
TatI WGTACW 4 cut(s) 753, 1509, 1707, 2397
Tru1I TTAA 5 cut(s) 915, 1718, 1877, 2198, 2394
Tru9I TTAA 5 cut(s) 915, 1718, 1877, 2198, 2394
TscAI CASTG 7 cut(s) 337, 607, 1174, 1384, 1978, 2034, 2053
TseFI GTSAC 1 cut(s) 1934
TseI GCWGC 9 cut(s) 219, 411, 560, 574, 817, 1231, 1234, 1478, 2051
Tsp45I GTSAC 1 cut(s) 1934
TspGWI ACGGA 1 cut(s) 326
TspRI CASTG 7 cut(s) 337, 607, 1174, 1384, 1978, 2034, 2053
Tth111I GACNNNGTC 1 cut(s) 13
Van91I CCANNNNNTGG 2 cut(s) 1641, 1994
Vha464I CTTAAG 2 cut(s) 1717, 2393
VpaK11BI GGWCC 1 cut(s) 229
VspI ATTAAT 1 cut(s) 1877
XagI CCTNNNNNAGG 1 cut(s) 1996
XapI RAATTY 7 cut(s) 175, 683, 714, 1121, 1599, 1664, 1766
XceI RCATGY 2 cut(s) 1183, 1386
XcmI CCANNNNNNNNNTGG 1 cut(s) 1970
XmaJI CCTAGG 1 cut(s) 1864
XmiI GTMKAC 3 cut(s) 549, 811, 1187
XmnI GAANNNNTTC 1 cut(s) 683
XspI CTAG 8 cut(s) 164, 471, 644, 1137, 1454, 1502, 1784, 1865
ZrmI AGTACT 1 cut(s) 2399
Zsp2I ATGCAT 1 cut(s) 740
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.