pycom04g16410

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0001553
Physical Location & Seq
Forward (+)
19052890 .. 19055233
2344 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1344 bp
ATGGTGAAGTTCTTGTGGATTGGCATTGTAACAGCACTTGTGTTCAGAACTTGGGTTGATGGGTTTGTGGAAGAGAAGAATCTTGATGAGACGGTGCCGTCTTTGTTGGAGACCGATGGAAGCTCCAAGGCTTTTAATGACACACTACTCATGGTAGAGCTAACACTCGTCCAAGAAGCTGCTGCTAAAGGAGCAGTCTGCTTGGATGGAACAGCACCTGCTTATCATTTACATCGTGGATATGGATCGGGAGCGAACAGTTGGCTCATTCACCTTCAGGGAGGAGCATGGTGTAACGACACCAAAAGTTGTTCTAACCGCACAACTTCACTTTTCGGATTGGGATCATCAGCGTACATGGAACCATATATGGCATTTTCTGGAATACTAAGCAACCAATCTGAGGAAAATCCCGGTTTCTCCTCAAACTTAATGGTACATTGGACTGCAGAGTTTTTCAACTGGAATAGAGTAGAGCTCCGCTATTGCGACGGTGCTTCATTTTCTGGGGACAGCGAAAGTCAGGTTGGTGGGATGATCTTGCCATTTACATTTTTCCACAAAAGTATGATAGGGGTTGTAGTAGTGCTGCAGCTATTGGAAGCAAAACTGCAATATAGAGGACAGCGTATATGGTTATCTGCAATGGACGATTTGATGTCAAAGGGAATGCGTGACGCGAAAAATGCTCTTCTTTGTGGTTGCTCTGCCGGCGGTCTGTCAACAATTTTGCACTGTGATGAGTTTCGGGGCATGTTTCCCACAAGTACAAAAGTGAAGTGCCTGAGTGATGCTGGATTTTTTCTTGACGCGGTTGATATTTCCGGTGAACGCACACTGAGGGATTATTATAGCGGTGTTGTAAGCTTGCAGGGGGTGCAAGAAAATCTGCTGCGATTCTGTACTAGTCACCTCGATCCTACTTCGTGCTTCTTCCCGGAGAACTTAATTGTGAACATTACAACCCCGCTATTTATACTCAATTCAGCATACGATTCATGGCAGATCAATAACAGTTTCGTTCCACCATCAGCAGATCGGTGCAGCGAGTGGGAGAGTTGCAGATTTAACCTTGCAAGTTGTTCTGCATCACAGATGCAAATTCTGCAAGGATTCAGGAATCAAATGCTGAATGCAGTAAAACAATTCTCAATCCCTAATCAAAATGGGATGTTCATAAATTCGTGTTTAGTGCACTGTCAAACGAAGGAAAGTGCATTGTTTGCTCATGAATTTCCAGCTCTTGGAAACAAGACAATTGGAGAAGCTCTTGGTGATTGGTATTTTGATAGAGCAGAGGTTAAGGTTGTTGATGAAACTTGCTGCGATCTGGCTCTCGAATGA

Protein Analysis

448

Amino Acids

49.49

Weight (kDa)

4.96

Isoelectric Point (pI)

38.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 50 - 438 2.7e-141 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 226
AasI GACNNNNNNGTC 1 cut(s) 97
Acc36I ACCTGC 1 cut(s) 226
AccB1I GGYRCC 1 cut(s) 94
AccB7I CCANNNNNTGG 1 cut(s) 1244
AccII CGCG 2 cut(s) 680, 812
AciI CCGC 6 cut(s) 319, 481, 714, 812, 855, 968
AclWI GGATC 3 cut(s) 253, 352, 911
AcsI RAATTY 3 cut(s) 1101, 1180, 1232
AcuI CTGAAG 1 cut(s) 260
AfaI GTAC 4 cut(s) 356, 438, 769, 904
AfiI CCNNNNNNNGG 2 cut(s) 403, 1244
AgsI TTSAA 1 cut(s) 460
AhlI ACTAGT 1 cut(s) 905
AjuI GAANNNNNNNTTGG 2 cut(s) 510, 542
AluBI AGCT 8 cut(s) 123, 160, 179, 478, 595, 867, 1241, 1268
AluI AGCT 8 cut(s) 123, 160, 179, 478, 595, 867, 1241, 1268
Alw21I GWGCWC 2 cut(s) 480, 1197
Alw26I GTCTC 2 cut(s) 83, 104
Alw44I GTGCAC 1 cut(s) 1193
AlwI GGATC 3 cut(s) 253, 352, 911
AlwNI CAGNNNCTG 1 cut(s) 218
ApaLI GTGCAC 1 cut(s) 1193
ApeKI GCWGC 7 cut(s) 179, 182, 589, 592, 892, 1044, 1323
ApoI RAATTY 3 cut(s) 1101, 1180, 1232
AsuC2I CCSGG 2 cut(s) 414, 938
AsuHPI GGTGA 5 cut(s) 16, 263, 839, 902, 1286
BaeGI GKGCMC 1 cut(s) 1197
BanI GGYRCC 1 cut(s) 94
BanII GRGCYC 1 cut(s) 480
Bbv12I GWGCWC 2 cut(s) 480, 1197
BbvI GCAGC 7 cut(s) 166, 169, 576, 604, 879, 1056, 1310
BccI CCATC 4 cut(s) 53, 110, 200, 1036
BceAI ACGGC 1 cut(s) 82
BcnI CCSGG 2 cut(s) 414, 938
BcoDI GTCTC 2 cut(s) 83, 104
BcuI ACTAGT 1 cut(s) 905
BfaI CTAG 1 cut(s) 906
BfmI CTRYAG 2 cut(s) 447, 590
BfuAI ACCTGC 1 cut(s) 226
BisI GCNGC 7 cut(s) 180, 183, 590, 593, 893, 1045, 1324
BlsI GCNGC 7 cut(s) 181, 184, 591, 594, 894, 1046, 1325
Bme1390I CCNGG 2 cut(s) 414, 938
BmiI GGNNCC 2 cut(s) 96, 363
BmrFI CCNGG 2 cut(s) 414, 938
BmsI GCATC 3 cut(s) 781, 1086, 1097
BplI GAGNNNNNCTC 2 cut(s) 462, 494
BpuMI CCSGG 2 cut(s) 414, 938
BsaBI GATNNNNATC 2 cut(s) 244, 343
BsaI GGTCTC 1 cut(s) 104
BsaJI CCNNGG 1 cut(s) 126
BsaWI WCCGGW 1 cut(s) 824
Bsc4I CCNNNNNNNGG 2 cut(s) 403, 1244
Bse118I RCCGGY 1 cut(s) 710
Bse1I ACTGG 1 cut(s) 467
Bse3DI GCAATG 1 cut(s) 651
Bse8I GATNNNNATC 2 cut(s) 244, 343
BseDI CCNNGG 1 cut(s) 126
BseGI GGATG 3 cut(s) 211, 540, 1176
BseJI GATNNNNATC 2 cut(s) 244, 343
BseLI CCNNNNNNNGG 2 cut(s) 403, 1244
BseMI GCAATG 1 cut(s) 651
BseMII CTCAG 3 cut(s) 393, 776, 830
BseNI ACTGG 1 cut(s) 467
BseRI GAGGAG 2 cut(s) 297, 412
BseSI GKGCMC 1 cut(s) 1197
BseXI GCAGC 7 cut(s) 166, 169, 576, 604, 879, 1056, 1310
BsgI GTGCAG 1 cut(s) 1063
Bsh1236I CGCG 2 cut(s) 680, 812
BshNI GGYRCC 1 cut(s) 94
BsiHKAI GWGCWC 2 cut(s) 480, 1197
BsiSI CCGG 4 cut(s) 414, 711, 825, 938
BslFI GGGAC 1 cut(s) 524
BslI CCNNNNNNNGG 2 cut(s) 403, 1244
BsmAI GTCTC 2 cut(s) 83, 104
BsmBI CGTCTC 1 cut(s) 83
BsmFI GGGAC 1 cut(s) 524
BsmI GAATGC 2 cut(s) 675, 1138
Bso31I GGTCTC 1 cut(s) 104
Bsp1286I GDGCHC 2 cut(s) 480, 1197
Bsp143I GATC 7 cut(s) 245, 344, 537, 916, 1005, 1036, 1327
BspACI CCGC 6 cut(s) 319, 481, 714, 812, 855, 968
BspCNI CTCAG 3 cut(s) 394, 777, 831
BspFNI CGCG 2 cut(s) 680, 812
BspHI TCATGA 1 cut(s) 1228
BspLI GGNNCC 2 cut(s) 96, 363
BspMAI CTGCAG 2 cut(s) 451, 594
BspMI ACCTGC 1 cut(s) 226
BspPI GGATC 3 cut(s) 253, 352, 911
BspQI GCTCTTC 1 cut(s) 696
BspT107I GGYRCC 1 cut(s) 94
BspTNI GGTCTC 1 cut(s) 104
BsrDI GCAATG 1 cut(s) 651
BsrFI RCCGGY 1 cut(s) 710
BsrI ACTGG 1 cut(s) 467
BssAI RCCGGY 1 cut(s) 710
BssECI CCNNGG 1 cut(s) 126
BssMI GATC 7 cut(s) 245, 344, 537, 916, 1005, 1036, 1327
BssT1I CCWWGG 1 cut(s) 126
Bst4CI ACNGT 6 cut(s) 94, 260, 494, 737, 1016, 1199
Bst6I CTCTTC 2 cut(s) 66, 696
BstAPI GCANNNNNTGC 3 cut(s) 877, 1105, 1223
BstC8I GCNNGC 2 cut(s) 712, 869
BstDEI CTNAG 4 cut(s) 389, 402, 785, 839
BstF5I GGATG 3 cut(s) 211, 540, 1176
BstFNI CGCG 2 cut(s) 680, 812
BstKTI GATC 7 cut(s) 248, 347, 540, 919, 1008, 1039, 1330
BstMAI GTCTC 2 cut(s) 83, 104
BstMBI GATC 7 cut(s) 245, 344, 537, 916, 1005, 1036, 1327
BstMWI GCNNNNNNNGC 6 cut(s) 191, 686, 711, 877, 1105, 1223
BstNSI RCATGY 1 cut(s) 757
BstSCI CCNGG 2 cut(s) 412, 936
BstSFI CTRYAG 2 cut(s) 447, 590
BstSLI GKGCMC 1 cut(s) 1197
BstUI CGCG 2 cut(s) 680, 812
BstV1I GCAGC 7 cut(s) 166, 169, 576, 604, 879, 1056, 1310
BtsCI GGATG 3 cut(s) 211, 540, 1176
BtsIMutI CAGTG 3 cut(s) 733, 836, 1195
BveI ACCTGC 1 cut(s) 226
Cac8I GCNNGC 2 cut(s) 712, 869
CaiI CAGNNNCTG 1 cut(s) 218
CciI TCATGA 1 cut(s) 1228
Cfr10I RCCGGY 1 cut(s) 710
CseI GACGC 2 cut(s) 686, 818
Csp6I GTAC 4 cut(s) 355, 437, 768, 903
CviAII CATG 6 cut(s) 151, 288, 358, 754, 999, 1229
CviQI GTAC 4 cut(s) 355, 437, 768, 903
DdeI CTNAG 4 cut(s) 389, 402, 785, 839
DpnI GATC 7 cut(s) 247, 346, 539, 918, 1007, 1038, 1329
DpnII GATC 7 cut(s) 245, 344, 537, 916, 1005, 1036, 1327
DrdI GACNNNNNNGTC 1 cut(s) 97
DseDI GACNNNNNNGTC 1 cut(s) 97
Eam1104I CTCTTC 2 cut(s) 66, 696
EarI CTCTTC 2 cut(s) 66, 696
Ecl136II GAGCTC 1 cut(s) 478
Eco130I CCWWGG 1 cut(s) 126
Eco24I GRGCYC 1 cut(s) 480
Eco31I GGTCTC 1 cut(s) 104
Eco53kI GAGCTC 1 cut(s) 478
Eco57I CTGAAG 1 cut(s) 260
EcoICRI GAGCTC 1 cut(s) 478
EcoT14I CCWWGG 1 cut(s) 126
EcoT38I GRGCYC 1 cut(s) 480
ErhI CCWWGG 1 cut(s) 126
Esp3I CGTCTC 1 cut(s) 83
FaeI CATG 6 cut(s) 154, 291, 361, 757, 1002, 1232
FaqI GGGAC 1 cut(s) 524
FatI CATG 6 cut(s) 150, 287, 357, 753, 998, 1228
FauI CCCGC 1 cut(s) 975
Fnu4HI GCNGC 7 cut(s) 180, 183, 590, 593, 893, 1045, 1324
FokI GGATG 3 cut(s) 218, 547, 1183
FriOI GRGCYC 1 cut(s) 480
Fsp4HI GCNGC 7 cut(s) 180, 183, 590, 593, 893, 1045, 1324
FspBI CTAG 1 cut(s) 906
GluI GCNGC 7 cut(s) 180, 183, 590, 593, 893, 1045, 1324
HapII CCGG 4 cut(s) 414, 711, 825, 938
HgaI GACGC 2 cut(s) 686, 818
Hin1II CATG 6 cut(s) 154, 291, 361, 757, 1002, 1232
HincII GTYRAC 1 cut(s) 723
HindII GTYRAC 1 cut(s) 723
HindIII AAGCTT 1 cut(s) 865
HinfI GANTC 5 cut(s) 79, 897, 995, 1113, 1120
HpaII CCGG 4 cut(s) 414, 711, 825, 938
HphI GGTGA 5 cut(s) 16, 263, 839, 902, 1286
Hpy166II GTNNAC 4 cut(s) 723, 830, 955, 1195
Hpy188I TCNGA 3 cut(s) 47, 338, 403
Hpy188III TCNNGA 7 cut(s) 83, 249, 381, 806, 1117, 1229, 1337
Hpy8I GTNNAC 4 cut(s) 723, 830, 955, 1195
Hpy99I CGWCG 1 cut(s) 494
HpyAV CCTTC 2 cut(s) 284, 1201
HpyCH4III ACNGT 6 cut(s) 94, 260, 494, 737, 1016, 1199
HpyF10VI GCNNNNNNNGC 6 cut(s) 191, 686, 711, 877, 1105, 1223
HpyF3I CTNAG 4 cut(s) 389, 402, 785, 839
Hsp92II CATG 6 cut(s) 154, 291, 361, 757, 1002, 1232
KroI GCCGGC 1 cut(s) 710
KroNI GCCGGC 1 cut(s) 712
Kzo9I GATC 7 cut(s) 245, 344, 537, 916, 1005, 1036, 1327
LguI GCTCTTC 1 cut(s) 696
LmnI GCTCC 5 cut(s) 128, 191, 251, 284, 483
Lsp1109I GCAGC 7 cut(s) 166, 169, 576, 604, 879, 1056, 1310
LweI GCATC 3 cut(s) 781, 1086, 1097
MaeI CTAG 1 cut(s) 906
MaeIII GTNAC 4 cut(s) 28, 293, 674, 908
MalI GATC 7 cut(s) 247, 346, 539, 918, 1007, 1038, 1329
MboI GATC 7 cut(s) 245, 344, 537, 916, 1005, 1036, 1327
MboII GAAGA 4 cut(s) 83, 88, 683, 925
MfeI CAATTG 1 cut(s) 1257
MhlI GDGCHC 2 cut(s) 480, 1197
MluCI AATT 8 cut(s) 726, 948, 982, 1101, 1145, 1180, 1232, 1257
MmeI TCCRAC 1 cut(s) 87
MnlI CCTC 7 cut(s) 275, 397, 433, 614, 834, 923, 1291
MroNI GCCGGC 1 cut(s) 710
MseI TTAA 5 cut(s) 135, 431, 947, 1068, 1302
MslI CAYNNNNRTG 1 cut(s) 738
MspI CCGG 4 cut(s) 414, 711, 825, 938
MspR9I CCNGG 2 cut(s) 414, 938
MunI CAATTG 1 cut(s) 1257
Mva1269I GAATGC 2 cut(s) 675, 1138
MvnI CGCG 2 cut(s) 680, 812
MwoI GCNNNNNNNGC 6 cut(s) 191, 686, 711, 877, 1105, 1223
NaeI GCCGGC 1 cut(s) 712
NciI CCSGG 2 cut(s) 414, 938
NdeII GATC 7 cut(s) 245, 344, 537, 916, 1005, 1036, 1327
NgoMIV GCCGGC 1 cut(s) 710
NlaIII CATG 6 cut(s) 154, 291, 361, 757, 1002, 1232
NlaIV GGNNCC 2 cut(s) 96, 363
NmuCI GTSAC 2 cut(s) 674, 908
NspI RCATGY 1 cut(s) 757
PagI TCATGA 1 cut(s) 1228
PaqCI CACCTGC 1 cut(s) 226
PciSI GCTCTTC 1 cut(s) 696
PctI GAATGC 2 cut(s) 675, 1138
PdiI GCCGGC 1 cut(s) 712
PfeI GAWTC 5 cut(s) 79, 897, 995, 1113, 1120
PflMI CCANNNNNTGG 1 cut(s) 1244
PfoI TCCNGGA 1 cut(s) 936
PkrI GCNGC 7 cut(s) 181, 184, 591, 594, 894, 1046, 1325
Psp124BI GAGCTC 1 cut(s) 480
PspN4I GGNNCC 2 cut(s) 96, 363
PstI CTGCAG 2 cut(s) 451, 594
PstNI CAGNNNCTG 1 cut(s) 218
RsaI GTAC 4 cut(s) 356, 438, 769, 904
RsaNI GTAC 4 cut(s) 355, 437, 768, 903
RseI CAYNNNNRTG 1 cut(s) 738
SacI GAGCTC 1 cut(s) 480
SapI GCTCTTC 1 cut(s) 696
SaqAI TTAA 5 cut(s) 135, 431, 947, 1068, 1302
SatI GCNGC 7 cut(s) 180, 183, 590, 593, 893, 1045, 1324
Sau3AI GATC 7 cut(s) 245, 344, 537, 916, 1005, 1036, 1327
ScrFI CCNGG 2 cut(s) 414, 938
SduI GDGCHC 2 cut(s) 480, 1197
SfaNI GCATC 3 cut(s) 781, 1086, 1097
SfcI CTRYAG 2 cut(s) 447, 590
SmiMI CAYNNNNRTG 1 cut(s) 738
SpeI ACTAGT 1 cut(s) 905
Sse9I AATT 8 cut(s) 726, 948, 982, 1101, 1145, 1180, 1232, 1257
SsiI CCGC 6 cut(s) 319, 481, 714, 812, 855, 968
SspMI CTAG 1 cut(s) 906
SstI GAGCTC 1 cut(s) 480
StyD4I CCNGG 2 cut(s) 412, 936
StyI CCWWGG 1 cut(s) 126
TaaI ACNGT 6 cut(s) 94, 260, 494, 737, 1016, 1199
TaqI TCGA 2 cut(s) 915, 1338
TaqII GACCGA 1 cut(s) 128
TasI AATT 8 cut(s) 726, 948, 982, 1101, 1145, 1180, 1232, 1257
TatI WGTACW 2 cut(s) 767, 902
TfiI GAWTC 5 cut(s) 79, 897, 995, 1113, 1120
Tru1I TTAA 5 cut(s) 135, 431, 947, 1068, 1302
Tru9I TTAA 5 cut(s) 135, 431, 947, 1068, 1302
TscAI CASTG 3 cut(s) 740, 843, 1202
TseFI GTSAC 2 cut(s) 674, 908
TseI GCWGC 7 cut(s) 179, 182, 589, 592, 892, 1044, 1323
Tsp45I GTSAC 2 cut(s) 674, 908
TspDTI ATGAA 5 cut(s) 489, 987, 1165, 1245, 1329
TspRI CASTG 3 cut(s) 740, 843, 1202
Van91I CCANNNNNTGG 1 cut(s) 1244
VneI GTGCAC 1 cut(s) 1193
XapI RAATTY 3 cut(s) 1101, 1180, 1232
XceI RCATGY 1 cut(s) 757
XspI CTAG 1 cut(s) 906
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.