pycom07g02180

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0002404
Physical Location & Seq
Reverse (-)
1747918 .. 1754259
6342 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1902 bp
ATGGGCTCCTGTAGGGTTTCCAATTATTCATTCGTTTTCTTCATATTGTTTCTGTGTTCTTTTGCTTCTTCTTCTTGTTCATCATCCTTGCACCCTTTGGTTTTCAACTCCAAAGTTGAACACCTTAATCACACAGCAATATCTGAGTTTAGGGTTCTTAACAGAAGATTTCTAGGCGACTGTCCGAATCCGAGCCCTTATCTCCAAATTAGTACTAATTCAAGTGCTTCTGGCCTTGGAGATGAAGAATATGTGAGTGTCAATATTAGCGGCGTCCTGCGTCCTTCCAAAGATGATTGGGTCGCCATGATATCACCTTCTCATTCTGATTTATCAAGCTGCCTTGCCAATGGAATTCTTTATGCACAAACCGGAGATTTTGAAAAACTACCTCTTCTCTGCCATTATCCTGTCAAGGCAGCATACATGAGCAATGATCCTGACTATGTCAGCTGCAAGAAGAAGGAATGCCAAAAGTATCAGAATGGTCGATGCATGGTGTCCACCTGTGGTGGTTCATTATTATTTCATGTTATCAACATCAGAACCGACATTGAATTTGTGTTGTTCTCCGGTGGATTTGAGGAACCTTGTATTCTCAAGAAGTCAAACCCTCTGAAATTTACCAATCCGAATAAGCCATTATATGGACATCTCTCAAGCATCGATTCAACTGGAACATCGATGAGATTAACATGGGTTAGTGGACATAAGGAACCTCAGCAAGTGCAATATGGCAACAGAAAAAAACAGACATCAGGAGTTACTACATTTTCACAAGACGATATGAAAAGTTCAGCTATACCAAGTCCAGCCAAGGACTTCGGCTGGCATGATCCGGGCTTCATTCACACAGTAGTAATGAGAGGACTTAAGCCTTCATCTAGTTTCTCTTATAGATACGGAAGCGATTCAGTTGGTTGGAGTGATCAAATCCAATTCCAAACTCCACCAGCAGGAGGATCTGATGAACTCAAATTTCTTGCATTTGGCGACATGGGAAAGGCTCCTCGTGATTCTTCGGCGATGCATTACATTCAGCCAGGATCTCTTTCAGTGATTCAAGCCGTGGCCGATGAAATAAATTCCGGGAATGTAGACTCTATCTTCCACATTGGAGACATTAGCTATGCTACAGGATTTTTAGTTGAATGGGATCTTTTCCTTCAACAAATCAGTCCAGTCGCTTCTCGAGTTTCTTACATGACAGCAATAGGAAACCATGAGAGGGACTATATAGACACTGGATCGGTTTATATTTTACCAGATTCAGGTGGAGAATCTGGAGTCCCTTATGAGACTTACTTCCCTATGCCAACCTCAGCAAAGGATAAGCCATGGTATTCCATAGAACAAGCAGGTGTTCACTTTACGGTGATTTCAACAGAGCATGACTGGTCCACAAATTCTGAGCAGTATCAATGGATGAGAAAGGACATGGCTTCAGTTGATCGATCTAAAACTCCTTGGTTGATTTTTATGGGGCATAGACCCATGTATTCCTCCTCACCTGGATTCCTCAATGTTGATAAAATATTTGTCAACGAAGTGGAACCATTACTGCTCGAGAGCAAAGTTGATTTAGTCCTATTTGGTCATGTCCATAACTACGAGCGAACTTGTGCGGTTTATAAGAGTGAATGCAAGGGATTGCCTCAAAAAGATGAAAATGGGATTGACACATATGATCATAGCAACTACAGTGCCCCAGTGCACGCTGTAATTGGTATGGCTGGCTTTACCTTGGACGGGTTCTCAAGTGGTGTTAATAATCCTTGGAGTTTATCCAAGATTTCTGAATTTGGTTATCTAAGAGGACATGCGACAAAGAAAGAGATGAAATTAGAGCTTGTGAATGCAGATACAAGAAAGGTTGAAGACAGATTCCGCATTACAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003993 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0044237
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

634

Amino Acids

70.46

Weight (kDa)

6.09

Isoelectric Point (pI)

44.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
fn3_PAP PF17808 79 - 210 3.5e-34 Fn3-like domain from Purple Acid Phosphatase
Pur_ac_phosph_N PF16656 216 - 316 2.5e-15 Purple acid Phosphatase, N-terminal domain
Metallophos PF00149 325 - 537 1.7e-18 Calcineurin-like phosphoesterase
Metallophos_C PF14008 569 - 627 1.3e-07 Iron/zinc purple acid phosphatase-like protein C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1632
AarI CACCTGC 1 cut(s) 1349
Acc36I ACCTGC 1 cut(s) 1349
AccB7I CCANNNNNTGG 1 cut(s) 647
AccI GTMKAC 1 cut(s) 1098
AciI CCGC 3 cut(s) 270, 1625, 1888
AclWI GGATC 6 cut(s) 431, 830, 970, 1054, 1164, 1255
AcoI YGGCCR 1 cut(s) 1071
AcsI RAATTY 7 cut(s) 354, 557, 620, 977, 1084, 1405, 1799
AcuI CTGAAG 1 cut(s) 1428
AcyI GRCGYC 1 cut(s) 273
AfaI GTAC 1 cut(s) 214
AfiI CCNNNNNNNGG 6 cut(s) 647, 956, 959, 1228, 1271, 1749
AflII CTTAAG 1 cut(s) 872
AjnI CCWGG 2 cut(s) 1042, 1510
AjuI GAANNNNNNNTTGG 2 cut(s) 14, 46
AluBI AGCT 5 cut(s) 339, 453, 800, 1128, 1849
AluI AGCT 5 cut(s) 339, 453, 800, 1128, 1849
Alw21I GWGCWC 1 cut(s) 1716
Alw26I GTCTC 2 cut(s) 1113, 1292
Alw44I GTGCAC 1 cut(s) 1712
AlwI GGATC 6 cut(s) 431, 830, 970, 1054, 1164, 1255
Ama87I CYCGRG 2 cut(s) 1191, 1565
AoxI GGCC 2 cut(s) 232, 1071
ApaLI GTGCAC 1 cut(s) 1712
ApeKI GCWGC 3 cut(s) 339, 419, 453
ApoI RAATTY 7 cut(s) 354, 557, 620, 977, 1084, 1405, 1799
ArsI GACNNNNNNTTYG 2 cut(s) 542, 574
Asp700I GAANNNNTTC 1 cut(s) 910
AspS9I GGNCC 1 cut(s) 1398
AsuC2I CCSGG 2 cut(s) 840, 1090
AsuHPI GGTGA 3 cut(s) 306, 1387, 1500
AvaI CYCGRG 2 cut(s) 1191, 1565
AvaII GGWCC 1 cut(s) 1398
BaeGI GKGCMC 2 cut(s) 1708, 1716
BanII GRGCYC 2 cut(s) 8, 197
BauI CACGAG 1 cut(s) 1011
BbsI GAAGAC 1 cut(s) 1884
Bbv12I GWGCWC 1 cut(s) 1716
BbvCI CCTCAGC 2 cut(s) 720, 1321
BbvI GCAGC 3 cut(s) 326, 431, 440
BceAI ACGGC 1 cut(s) 1052
BciT130I CCWGG 2 cut(s) 1044, 1512
BclI TGATCA 2 cut(s) 928, 1687
BcnI CCSGG 2 cut(s) 840, 1090
BcoDI GTCTC 2 cut(s) 1113, 1292
BfaI CTAG 2 cut(s) 173, 885
BfmI CTRYAG 3 cut(s) 10, 1134, 1699
BfrI CTTAAG 1 cut(s) 872
BfuAI ACCTGC 1 cut(s) 1349
BisI GCNGC 4 cut(s) 271, 340, 420, 454
BlsI GCNGC 4 cut(s) 272, 341, 421, 455
BmcAI AGTACT 1 cut(s) 214
Bme1390I CCNGG 4 cut(s) 840, 1044, 1090, 1512
Bme18I GGWCC 1 cut(s) 1398
BmeT110I CYCGRG 2 cut(s) 1191, 1565
BmgT120I GGNCC 1 cut(s) 1398
BmiI GGNNCC 5 cut(s) 7, 588, 717, 1008, 1554
BmrFI CCNGG 4 cut(s) 840, 1044, 1090, 1512
BmrI ACTGGG 1 cut(s) 1703
BmsI GCATC 3 cut(s) 482, 672, 1017
BmuI ACTGGG 1 cut(s) 1703
BpiI GAAGAC 1 cut(s) 1884
BpmI CTGGAG 1 cut(s) 1305
Bpu10I CCTNAGC 2 cut(s) 720, 1321
BpuEI CTTGAG 3 cut(s) 584, 643, 1741
BpuMI CCSGG 2 cut(s) 840, 1090
Bsa29I ATCGAT 3 cut(s) 666, 683, 1453
BsaHI GRCGYC 1 cut(s) 273
BsaJI CCNNGG 7 cut(s) 235, 816, 1068, 1337, 1466, 1743, 1775
BsaWI WCCGGW 2 cut(s) 371, 572
Bsc4I CCNNNNNNNGG 6 cut(s) 647, 956, 959, 1228, 1271, 1749
Bse1I ACTGG 5 cut(s) 679, 1181, 1249, 1400, 1709
Bse3DI GCAATG 1 cut(s) 439
BseBI CCWGG 2 cut(s) 1044, 1512
BseCI ATCGAT 3 cut(s) 666, 683, 1453
BseDI CCNNGG 7 cut(s) 235, 816, 1068, 1337, 1466, 1743, 1775
BseGI GGATG 2 cut(s) 83, 1431
BseLI CCNNNNNNNGG 6 cut(s) 647, 956, 959, 1228, 1271, 1749
BseMI GCAATG 1 cut(s) 439
BseMII CTCAG 4 cut(s) 135, 734, 1335, 1401
BseNI ACTGG 5 cut(s) 679, 1181, 1249, 1400, 1709
BseRI GAGGAG 2 cut(s) 999, 1495
BseSI GKGCMC 2 cut(s) 1708, 1716
BseXI GCAGC 3 cut(s) 326, 431, 440
BshFI GGCC 2 cut(s) 234, 1073
BshVI ATCGAT 3 cut(s) 666, 683, 1453
BsiHKAI GWGCWC 1 cut(s) 1716
BsiHKCI CYCGRG 2 cut(s) 1191, 1565
BsiSI CCGG 4 cut(s) 372, 573, 839, 1089
BslFI GGGAC 2 cut(s) 1244, 1274
BslI CCNNNNNNNGG 6 cut(s) 647, 956, 959, 1228, 1271, 1749
BsmAI GTCTC 2 cut(s) 1113, 1292
BsmFI GGGAC 2 cut(s) 1244, 1274
BsmI GAATGC 3 cut(s) 473, 1646, 1861
BsnI GGCC 2 cut(s) 234, 1073
BsoBI CYCGRG 2 cut(s) 1191, 1565
Bsp1286I GDGCHC 4 cut(s) 8, 197, 1708, 1716
Bsp19I CCATGG 1 cut(s) 1337
BspACI CCGC 3 cut(s) 270, 1625, 1888
BspANI GGCC 2 cut(s) 234, 1073
BspCNI CTCAG 4 cut(s) 136, 733, 1334, 1402
BspDI ATCGAT 3 cut(s) 666, 683, 1453
BspLI GGNNCC 5 cut(s) 7, 588, 717, 1008, 1554
BspMI ACCTGC 1 cut(s) 1349
BspPI GGATC 6 cut(s) 431, 830, 970, 1054, 1164, 1255
BspTI CTTAAG 1 cut(s) 872
BsrDI GCAATG 1 cut(s) 439
BsrI ACTGG 5 cut(s) 679, 1181, 1249, 1400, 1709
BssECI CCNNGG 7 cut(s) 235, 816, 1068, 1337, 1466, 1743, 1775
BssNI GRCGYC 1 cut(s) 273
BssSI CACGAG 1 cut(s) 1011
BssT1I CCWWGG 6 cut(s) 235, 816, 1337, 1466, 1743, 1775
Bst2BI CACGAG 1 cut(s) 1011
Bst2UI CCWGG 2 cut(s) 1044, 1512
Bst4CI ACNGT 4 cut(s) 182, 856, 1375, 1703
Bst6I CTCTTC 1 cut(s) 399
BstACI GRCGYC 1 cut(s) 273
BstAFI CTTAAG 1 cut(s) 872
BstC8I GCNNGC 3 cut(s) 830, 1716, 1735
BstDEI CTNAG 5 cut(s) 144, 720, 1321, 1410, 1811
BstDSI CCRYGG 2 cut(s) 1068, 1337
BstF5I GGATG 2 cut(s) 83, 1431
BstMAI GTCTC 2 cut(s) 1113, 1292
BstNI CCWGG 2 cut(s) 1044, 1512
BstNSI RCATGY 1 cut(s) 1823
BstSCI CCNGG 4 cut(s) 838, 1042, 1088, 1510
BstSFI CTRYAG 3 cut(s) 10, 1134, 1699
BstSLI GKGCMC 2 cut(s) 1708, 1716
BstV1I GCAGC 3 cut(s) 326, 431, 440
BstV2I GAAGAC 1 cut(s) 1884
BstX2I RGATCY 3 cut(s) 962, 1046, 1156
BstYI RGATCY 3 cut(s) 962, 1046, 1156
Bsu15I ATCGAT 3 cut(s) 666, 683, 1453
BsuRI GGCC 2 cut(s) 234, 1073
BsuTUI ATCGAT 3 cut(s) 666, 683, 1453
BtgI CCRYGG 2 cut(s) 1068, 1337
BtgZI GCGATG 1 cut(s) 1040
BtsCI GGATG 2 cut(s) 83, 1431
BtsIMutI CAGTG 4 cut(s) 1062, 1242, 1708, 1716
BveI ACCTGC 1 cut(s) 1349
Cac8I GCNNGC 3 cut(s) 830, 1716, 1735
Cfr13I GGNCC 1 cut(s) 1398
ClaI ATCGAT 3 cut(s) 666, 683, 1453
CseI GACGC 2 cut(s) 262, 269
Csp6I GTAC 1 cut(s) 213
CviQI GTAC 1 cut(s) 213
DdeI CTNAG 5 cut(s) 144, 720, 1321, 1410, 1811
EaeI YGGCCR 1 cut(s) 1071
Eam1104I CTCTTC 1 cut(s) 399
EarI CTCTTC 1 cut(s) 399
Eco130I CCWWGG 6 cut(s) 235, 816, 1337, 1466, 1743, 1775
Eco24I GRGCYC 2 cut(s) 8, 197
Eco32I GATATC 1 cut(s) 312
Eco47I GGWCC 1 cut(s) 1398
Eco57I CTGAAG 1 cut(s) 1428
Eco88I CYCGRG 2 cut(s) 1191, 1565
EcoRI GAATTC 1 cut(s) 354
EcoRII CCWGG 2 cut(s) 1042, 1510
EcoRV GATATC 1 cut(s) 312
EcoT14I CCWWGG 6 cut(s) 235, 816, 1337, 1466, 1743, 1775
EcoT22I ATGCAT 2 cut(s) 497, 1032
EcoT38I GRGCYC 2 cut(s) 8, 197
ErhI CCWWGG 6 cut(s) 235, 816, 1337, 1466, 1743, 1775
FaqI GGGAC 2 cut(s) 1244, 1274
FauNDI CATATG 1 cut(s) 1684
FbaI TGATCA 2 cut(s) 928, 1687
FblI GTMKAC 1 cut(s) 1098
Fnu4HI GCNGC 4 cut(s) 271, 340, 420, 454
FokI GGATG 2 cut(s) 70, 1438
FriOI GRGCYC 2 cut(s) 8, 197
Fsp4HI GCNGC 4 cut(s) 271, 340, 420, 454
FspBI CTAG 2 cut(s) 173, 885
GluI GCNGC 4 cut(s) 271, 340, 420, 454
GsuI CTGGAG 1 cut(s) 1305
HaeIII GGCC 2 cut(s) 234, 1073
HapII CCGG 4 cut(s) 372, 573, 839, 1089
HgaI GACGC 2 cut(s) 262, 269
Hin1I GRCGYC 1 cut(s) 273
HincII GTYRAC 1 cut(s) 1543
HindII GTYRAC 1 cut(s) 1543
HpaII CCGG 4 cut(s) 372, 573, 839, 1089
HphI GGTGA 3 cut(s) 306, 1387, 1500
Hpy166II GTNNAC 7 cut(s) 504, 707, 1099, 1366, 1401, 1543, 1714
Hpy188III TCNNGA 7 cut(s) 440, 601, 759, 1013, 1191, 1284, 1567
Hpy8I GTNNAC 7 cut(s) 504, 707, 1099, 1366, 1401, 1543, 1714
HpyAV CCTTC 5 cut(s) 294, 327, 457, 888, 1175
HpyCH4III ACNGT 4 cut(s) 182, 856, 1375, 1703
HpyF3I CTNAG 5 cut(s) 144, 720, 1321, 1410, 1811
Hsp92I GRCGYC 1 cut(s) 273
Ksp22I TGATCA 2 cut(s) 928, 1687
LmnI GCTCC 2 cut(s) 11, 1012
Lsp1109I GCAGC 3 cut(s) 326, 431, 440
LweI GCATC 3 cut(s) 482, 672, 1017
MaeI CTAG 2 cut(s) 173, 885
MaeIII GTNAC 1 cut(s) 763
MflI RGATCY 3 cut(s) 962, 1046, 1156
MhlI GDGCHC 4 cut(s) 8, 197, 1708, 1716
MlyI GAGTC 2 cut(s) 1094, 1296
MmeI TCCRAC 1 cut(s) 902
Mph1103I ATGCAT 2 cut(s) 497, 1032
MroXI GAANNNNTTC 1 cut(s) 910
MseI TTAA 5 cut(s) 126, 159, 692, 873, 1767
MspA1I CMGCKG 1 cut(s) 453
MspCI CTTAAG 1 cut(s) 872
MspI CCGG 4 cut(s) 372, 573, 839, 1089
MspR9I CCNGG 4 cut(s) 840, 1044, 1090, 1512
Mva1269I GAATGC 3 cut(s) 473, 1646, 1861
MvaI CCWGG 2 cut(s) 1044, 1512
NciI CCSGG 2 cut(s) 840, 1090
NcoI CCATGG 1 cut(s) 1337
NdeI CATATG 1 cut(s) 1684
NlaIV GGNNCC 5 cut(s) 7, 588, 717, 1008, 1554
NsiI ATGCAT 2 cut(s) 497, 1032
NspI RCATGY 1 cut(s) 1823
PaeR7I CTCGAG 2 cut(s) 1191, 1565
PaqCI CACCTGC 1 cut(s) 1349
PctI GAATGC 3 cut(s) 473, 1646, 1861
PdmI GAANNNNTTC 1 cut(s) 910
PfeI GAWTC 9 cut(s) 187, 668, 911, 1016, 1060, 1268, 1280, 1515, 1884
PflFI GACNNNGTC 1 cut(s) 446
PflMI CCANNNNNTGG 1 cut(s) 647
PfoI TCCNGGA 1 cut(s) 1088
PkrI GCNGC 4 cut(s) 272, 341, 421, 455
PleI GAGTC 2 cut(s) 1094, 1295
PpsI GAGTC 2 cut(s) 1094, 1295
PsiI TTATAA 1 cut(s) 1632
Psp6I CCWGG 2 cut(s) 1042, 1510
PspGI CCWGG 2 cut(s) 1042, 1510
PspN4I GGNNCC 5 cut(s) 7, 588, 717, 1008, 1554
PspPI GGNCC 1 cut(s) 1398
PsuI RGATCY 3 cut(s) 962, 1046, 1156
PsyI GACNNNGTC 1 cut(s) 446
PvuII CAGCTG 1 cut(s) 453
RsaI GTAC 1 cut(s) 214
RsaNI GTAC 1 cut(s) 213
SaqAI TTAA 5 cut(s) 126, 159, 692, 873, 1767
SatI GCNGC 4 cut(s) 271, 340, 420, 454
Sau96I GGNCC 1 cut(s) 1398
ScaI AGTACT 1 cut(s) 214
SchI GAGTC 2 cut(s) 1094, 1296
ScrFI CCNGG 4 cut(s) 840, 1044, 1090, 1512
SduI GDGCHC 4 cut(s) 8, 197, 1708, 1716
SfaNI GCATC 3 cut(s) 482, 672, 1017
SfcI CTRYAG 3 cut(s) 10, 1134, 1699
Sfr274I CTCGAG 2 cut(s) 1191, 1565
SinI GGWCC 1 cut(s) 1398
SlaI CTCGAG 2 cut(s) 1191, 1565
SmlI CTYRAG 6 cut(s) 599, 658, 872, 1191, 1565, 1756
SmoI CTYRAG 6 cut(s) 599, 658, 872, 1191, 1565, 1756
SsiI CCGC 3 cut(s) 270, 1625, 1888
SspI AATATT 2 cut(s) 265, 1536
SspMI CTAG 2 cut(s) 173, 885
StyD4I CCNGG 4 cut(s) 838, 1042, 1088, 1510
StyI CCWWGG 6 cut(s) 235, 816, 1337, 1466, 1743, 1775
TaaI ACNGT 4 cut(s) 182, 856, 1375, 1703
TaqI TCGA 6 cut(s) 490, 666, 683, 1192, 1453, 1566
TatI WGTACW 1 cut(s) 212
TauI GCSGC 1 cut(s) 273
TfiI GAWTC 9 cut(s) 187, 668, 911, 1016, 1060, 1268, 1280, 1515, 1884
Tru1I TTAA 5 cut(s) 126, 159, 692, 873, 1767
Tru9I TTAA 5 cut(s) 126, 159, 692, 873, 1767
TscAI CASTG 4 cut(s) 1062, 1249, 1708, 1716
TseI GCWGC 3 cut(s) 339, 419, 453
TspGWI ACGGA 1 cut(s) 918
TspRI CASTG 4 cut(s) 1062, 1249, 1708, 1716
Tth111I GACNNNGTC 1 cut(s) 446
Van91I CCANNNNNTGG 1 cut(s) 647
Vha464I CTTAAG 1 cut(s) 872
VneI GTGCAC 1 cut(s) 1712
VpaK11BI GGWCC 1 cut(s) 1398
XapI RAATTY 7 cut(s) 354, 557, 620, 977, 1084, 1405, 1799
XceI RCATGY 1 cut(s) 1823
XhoI CTCGAG 2 cut(s) 1191, 1565
XmiI GTMKAC 1 cut(s) 1098
XmnI GAANNNNTTC 1 cut(s) 910
XspI CTAG 2 cut(s) 173, 885
ZrmI AGTACT 1 cut(s) 214
Zsp2I ATGCAT 2 cut(s) 497, 1032
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.