pycom08g11830

Cysteine-rich receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0002863
Physical Location & Seq
Forward (+)
10090763 .. 10091607
845 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g11830.3

Sequence Viewer

Length: 537 bp
ATGCAGAAATGCAACCCAAAATTGCAGATTTTGGCATGTGGTTACATGGCACCTGAGTATGTGATGCGCGGACACTTTTCTGTTAAGTCCGATGTATATAGCTTCGGTGTGTTAGTTTTGGAGATAATTACTGGACAGAAAAATAGTTCTTTCCGTCACGGTGGGAACGTGGAGGACCTTCTAAGCTATGTAAGCAACACAAAAAGCTGGGCATGGAAAAGTTGGAATGAGGACACAGCTTCAAATTTAATAGATCCCATGTTGAAGAGTGGTTCAATACCAGAAATAATGAGATGCATCAACCTAGGATTATTATGTGTGCAACAAAATATAGCTGATAGGCCAACCATGGCTGCAGTTATTCTCATGCTTACTAGCAACTCTATCGATCTTCCAGTACCCTCACAACCAGCATTTTTTATGGATGGTGGCATTGAATCCTCATCTGACATGTCTTTGGCATGGGATGATAATTCTGGTGTGACAAGGTTAGATCTATCCAAAAGCAACTCTGTCAAATATATGCTGCATGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr Stress-antifung

Protein Analysis

179

Amino Acids

19.63

Weight (kDa)

5.53

Isoelectric Point (pI)

42.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 49
AccII CGCG 1 cut(s) 69
AciI CCGC 1 cut(s) 69
AclWI GGATC 1 cut(s) 248
AcsI RAATTY 1 cut(s) 244
AfaI GTAC 1 cut(s) 399
AflIII ACRYGT 1 cut(s) 450
AgsI TTSAA 4 cut(s) 243, 265, 276, 437
AluBI AGCT 5 cut(s) 102, 186, 207, 239, 335
AluI AGCT 5 cut(s) 102, 186, 207, 239, 335
AlwI GGATC 1 cut(s) 248
AoxI GGCC 1 cut(s) 341
ApeKI GCWGC 2 cut(s) 353, 526
ApoI RAATTY 1 cut(s) 244
AspA2I CCTAGG 1 cut(s) 304
AspLEI GCGC 1 cut(s) 69
AspS9I GGNCC 1 cut(s) 175
AvaII GGWCC 1 cut(s) 175
AvrII CCTAGG 1 cut(s) 304
BanI GGYRCC 1 cut(s) 49
BbvI GCAGC 2 cut(s) 340, 513
BccI CCATC 1 cut(s) 419
BcgI CGANNNNNNTGC 2 cut(s) 367, 401
BfaI CTAG 2 cut(s) 305, 375
BfmI CTRYAG 1 cut(s) 354
BglII AGATCT 1 cut(s) 493
BisI GCNGC 2 cut(s) 354, 527
BlnI CCTAGG 1 cut(s) 304
BlsI GCNGC 2 cut(s) 355, 528
Bme18I GGWCC 1 cut(s) 175
BmgT120I GGNCC 1 cut(s) 175
BmiI GGNNCC 1 cut(s) 51
BmsI GCATC 3 cut(s) 54, 284, 306
Bsa29I ATCGAT 1 cut(s) 387
BsaJI CCNNGG 2 cut(s) 304, 348
Bse1I ACTGG 2 cut(s) 136, 395
BseCI ATCGAT 1 cut(s) 387
BseDI CCNNGG 2 cut(s) 304, 348
BseGI GGATG 2 cut(s) 430, 472
BseMII CTCAG 1 cut(s) 45
BseNI ACTGG 2 cut(s) 136, 395
BseXI GCAGC 2 cut(s) 340, 513
BseYI CCCAGC 1 cut(s) 207
Bsh1236I CGCG 1 cut(s) 69
BshFI GGCC 1 cut(s) 343
BshNI GGYRCC 1 cut(s) 49
BshVI ATCGAT 1 cut(s) 387
BsnI GGCC 1 cut(s) 343
Bsp143I GATC 3 cut(s) 253, 388, 493
Bsp19I CCATGG 1 cut(s) 348
BspACI CCGC 1 cut(s) 69
BspANI GGCC 1 cut(s) 343
BspCNI CTCAG 1 cut(s) 46
BspDI ATCGAT 1 cut(s) 387
BspFNI CGCG 1 cut(s) 69
BspLI GGNNCC 1 cut(s) 51
BspMAI CTGCAG 1 cut(s) 358
BspPI GGATC 1 cut(s) 248
BspT107I GGYRCC 1 cut(s) 49
BsrI ACTGG 2 cut(s) 136, 395
BssECI CCNNGG 2 cut(s) 304, 348
BssMI GATC 3 cut(s) 253, 388, 493
BssT1I CCWWGG 2 cut(s) 304, 348
Bst4CI ACNGT 1 cut(s) 161
Bst6I CTCTTC 1 cut(s) 260
BstDEI CTNAG 2 cut(s) 54, 182
BstDSI CCRYGG 1 cut(s) 348
BstF5I GGATG 2 cut(s) 430, 472
BstFNI CGCG 1 cut(s) 69
BstHHI GCGC 1 cut(s) 69
BstKTI GATC 3 cut(s) 256, 391, 496
BstMBI GATC 3 cut(s) 253, 388, 493
BstMWI GCNNNNNNNGC 1 cut(s) 192
BstNSI RCATGY 2 cut(s) 39, 454
BstSFI CTRYAG 1 cut(s) 354
BstUI CGCG 1 cut(s) 69
BstV1I GCAGC 2 cut(s) 340, 513
BstX2I RGATCY 2 cut(s) 253, 493
BstYI RGATCY 2 cut(s) 253, 493
Bsu15I ATCGAT 1 cut(s) 387
BsuRI GGCC 1 cut(s) 343
BsuTUI ATCGAT 1 cut(s) 387
BtgI CCRYGG 1 cut(s) 348
BtsCI GGATG 2 cut(s) 430, 472
CfoI GCGC 1 cut(s) 69
Cfr13I GGNCC 1 cut(s) 175
ClaI ATCGAT 1 cut(s) 387
Csp6I GTAC 1 cut(s) 398
CviAII CATG 9 cut(s) 36, 46, 213, 259, 349, 367, 451, 462, 530
CviJI RGCY 7 cut(s) 102, 186, 207, 239, 335, 343, 353
CviKI_1 RGCY 7 cut(s) 102, 186, 207, 239, 335, 343, 353
CviQI GTAC 1 cut(s) 398
DdeI CTNAG 2 cut(s) 54, 182
DpnI GATC 3 cut(s) 255, 390, 495
DpnII GATC 3 cut(s) 253, 388, 493
Eam1104I CTCTTC 1 cut(s) 260
EarI CTCTTC 1 cut(s) 260
Eco130I CCWWGG 2 cut(s) 304, 348
Eco47I GGWCC 1 cut(s) 175
EcoO109I RGGNCCY 1 cut(s) 175
EcoT14I CCWWGG 2 cut(s) 304, 348
EcoT22I ATGCAT 1 cut(s) 299
ErhI CCWWGG 2 cut(s) 304, 348
FaeI CATG 9 cut(s) 39, 49, 216, 262, 352, 370, 454, 465, 533
FatI CATG 9 cut(s) 35, 45, 212, 258, 348, 366, 450, 461, 529
Fnu4HI GCNGC 2 cut(s) 354, 527
FokI GGATG 2 cut(s) 437, 479
Fsp4HI GCNGC 2 cut(s) 354, 527
FspBI CTAG 2 cut(s) 305, 375
GlaI GCGC 1 cut(s) 68
GluI GCNGC 2 cut(s) 354, 527
GsaI CCCAGC 1 cut(s) 211
HaeIII GGCC 1 cut(s) 343
HhaI GCGC 1 cut(s) 69
Hin1II CATG 9 cut(s) 39, 49, 216, 262, 352, 370, 454, 465, 533
Hin6I GCGC 1 cut(s) 67
HinP1I GCGC 1 cut(s) 67
HinfI GANTC 1 cut(s) 437
Hpy188I TCNGA 2 cut(s) 91, 448
HpyAV CCTTC 1 cut(s) 188
HpyCH4III ACNGT 1 cut(s) 161
HpyCH4IV ACGT 1 cut(s) 168
HpyCH4V TGCA 7 cut(s) 4, 12, 25, 297, 322, 356, 529
HpyF10VI GCNNNNNNNGC 1 cut(s) 192
HpyF3I CTNAG 2 cut(s) 54, 182
HpySE526I ACGT 1 cut(s) 168
Hsp92II CATG 9 cut(s) 39, 49, 216, 262, 352, 370, 454, 465, 533
HspAI GCGC 1 cut(s) 67
Kzo9I GATC 3 cut(s) 253, 388, 493
LpnPI CCDG 7 cut(s) 66, 117, 193, 294, 408, 423, 462
Lsp1109I GCAGC 2 cut(s) 340, 513
LweI GCATC 3 cut(s) 54, 284, 306
MaeI CTAG 2 cut(s) 305, 375
MaeII ACGT 1 cut(s) 168
MaeIII GTNAC 3 cut(s) 41, 155, 481
MalI GATC 3 cut(s) 255, 390, 495
MboI GATC 3 cut(s) 253, 388, 493
MboII GAAGA 2 cut(s) 277, 383
MflI RGATCY 2 cut(s) 253, 493
MluCI AATT 4 cut(s) 20, 126, 244, 472
MmeI TCCRAC 1 cut(s) 203
MnlI CCTC 4 cut(s) 166, 223, 412, 451
Mph1103I ATGCAT 1 cut(s) 299
MseI TTAA 2 cut(s) 84, 248
MvnI CGCG 1 cut(s) 69
MwoI GCNNNNNNNGC 1 cut(s) 192
NcoI CCATGG 1 cut(s) 348
NdeII GATC 3 cut(s) 253, 388, 493
NlaIII CATG 9 cut(s) 39, 49, 216, 262, 352, 370, 454, 465, 533
NlaIV GGNNCC 1 cut(s) 51
NmuCI GTSAC 2 cut(s) 155, 481
NsiI ATGCAT 1 cut(s) 299
NspI RCATGY 2 cut(s) 39, 454
PciI ACATGT 1 cut(s) 450
PcsI WCGNNNNNNNCGW 1 cut(s) 165
PfeI GAWTC 1 cut(s) 437
PkrI GCNGC 2 cut(s) 355, 528
PpuMI RGGWCCY 1 cut(s) 175
PscI ACATGT 1 cut(s) 450
Psp5II RGGWCCY 1 cut(s) 175
PspFI CCCAGC 1 cut(s) 207
PspN4I GGNNCC 1 cut(s) 51
PspPI GGNCC 1 cut(s) 175
PspPPI RGGWCCY 1 cut(s) 175
PstI CTGCAG 1 cut(s) 358
PsuI RGATCY 2 cut(s) 253, 493
RsaI GTAC 1 cut(s) 399
RsaNI GTAC 1 cut(s) 398
SaqAI TTAA 2 cut(s) 84, 248
SatI GCNGC 2 cut(s) 354, 527
Sau3AI GATC 3 cut(s) 253, 388, 493
Sau96I GGNCC 1 cut(s) 175
SfaNI GCATC 3 cut(s) 54, 284, 306
SfcI CTRYAG 1 cut(s) 354
SinI GGWCC 1 cut(s) 175
Sse9I AATT 4 cut(s) 20, 126, 244, 472
SsiI CCGC 1 cut(s) 69
SspMI CTAG 2 cut(s) 305, 375
StyI CCWWGG 2 cut(s) 304, 348
TaaI ACNGT 1 cut(s) 161
TaiI ACGT 1 cut(s) 171
TaqI TCGA 1 cut(s) 387
TasI AATT 4 cut(s) 20, 126, 244, 472
TfiI GAWTC 1 cut(s) 437
Tru1I TTAA 2 cut(s) 84, 248
Tru9I TTAA 2 cut(s) 84, 248
TseFI GTSAC 2 cut(s) 155, 481
TseI GCWGC 2 cut(s) 353, 526
Tsp45I GTSAC 2 cut(s) 155, 481
TspGWI ACGGA 1 cut(s) 143
VpaK11BI GGWCC 1 cut(s) 175
XapI RAATTY 1 cut(s) 244
XceI RCATGY 2 cut(s) 39, 454
XmaJI CCTAGG 1 cut(s) 304
XspI CTAG 2 cut(s) 305, 375
Zsp2I ATGCAT 1 cut(s) 299
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.