Rroxscaffold_1G00071700

Cysteine-rich receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0002863
Physical Location & Seq
Reverse (-)
92828119 .. 92828694
576 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00071700.1

Sequence Viewer

Length: 438 bp
ATGGCTCCAGAGTATGCCAACCATGGGCGTTTCTCTGTCAAATCGGATGTCTACAGTTTTGGTGTGTTAGTTCTTGAGATCGTGAGTAGCAAGAAAGTGAGCACTTTTCGAAATGGTGAGAATGAAGAGGACCTTCTCAGCTATGCATGGCGAAGTTGGAAAGAGGACACAGTTCATAATATCATAGATCCAATGTTGACACAGAGTTCAAGGATTGAAACAATGAGCTGTATTCACATTGGCTTACTTTGCGTCCAAGAAAATCTGAATAGGAGACCAACTATGTCTTCAGTTGTTTCCATGCTTAATAGCAATTCTGTTACACTCTCACAACCATTGCGACCTGGATATTTTTTGCAATATAATGATGAATCAGACATCACCGAACTGACAGAGTCAATAAATGAGGCTTCAATTACGAATCCATATCCTCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr Stress-antifung

Protein Analysis

145

Amino Acids

16.48

Weight (kDa)

5.0

Isoelectric Point (pI)

65.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 1 - 102 8.3e-10 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 51
AclWI GGATC 1 cut(s) 182
AcuI CTGAAG 1 cut(s) 273
AfiI CCNNNNNNNGG 1 cut(s) 24
AgsI TTSAA 3 cut(s) 210, 218, 414
AjnI CCWGG 1 cut(s) 343
AjuI GAANNNNNNNTTGG 2 cut(s) 271, 303
AluBI AGCT 2 cut(s) 141, 228
AluI AGCT 2 cut(s) 141, 228
Alw21I GWGCWC 1 cut(s) 104
Alw26I GTCTC 1 cut(s) 268
AlwI GGATC 1 cut(s) 182
ArsI GACNNNNNNTTYG 2 cut(s) 33, 65
AspS9I GGNCC 1 cut(s) 130
AsuHPI GGTGA 2 cut(s) 128, 373
AsuII TTCGAA 1 cut(s) 109
AvaII GGWCC 1 cut(s) 130
BbsI GAAGAC 1 cut(s) 279
Bbv12I GWGCWC 1 cut(s) 104
BciT130I CCWGG 1 cut(s) 345
BcoDI GTCTC 1 cut(s) 268
BfaI CTAG 1 cut(s) 436
BfmI CTRYAG 1 cut(s) 52
Bme1390I CCNGG 1 cut(s) 345
Bme18I GGWCC 1 cut(s) 130
BmgT120I GGNCC 1 cut(s) 130
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 1 cut(s) 345
BpiI GAAGAC 1 cut(s) 279
Bpu14I TTCGAA 1 cut(s) 109
BpuEI CTTGAG 1 cut(s) 95
BsaI GGTCTC 1 cut(s) 268
BsaJI CCNNGG 1 cut(s) 22
Bsc4I CCNNNNNNNGG 1 cut(s) 24
Bse3DI GCAATG 1 cut(s) 335
BseBI CCWGG 1 cut(s) 345
BseDI CCNNGG 1 cut(s) 22
BseGI GGATG 1 cut(s) 52
BseLI CCNNNNNNNGG 1 cut(s) 24
BseMI GCAATG 1 cut(s) 335
BseMII CTCAG 1 cut(s) 151
BsiHKAI GWGCWC 1 cut(s) 104
BslI CCNNNNNNNGG 1 cut(s) 24
BsmAI GTCTC 1 cut(s) 268
Bso31I GGTCTC 1 cut(s) 268
Bsp119I TTCGAA 1 cut(s) 109
Bsp1286I GDGCHC 1 cut(s) 104
Bsp143I GATC 2 cut(s) 78, 187
Bsp19I CCATGG 1 cut(s) 22
BspCNI CTCAG 1 cut(s) 150
BspLI GGNNCC 1 cut(s) 6
BspPI GGATC 1 cut(s) 182
BspT104I TTCGAA 1 cut(s) 109
BspTNI GGTCTC 1 cut(s) 268
BsrDI GCAATG 1 cut(s) 335
BssECI CCNNGG 1 cut(s) 22
BssMI GATC 2 cut(s) 78, 187
BssT1I CCWWGG 1 cut(s) 22
Bst2UI CCWGG 1 cut(s) 345
Bst4CI ACNGT 2 cut(s) 56, 172
Bst6I CTCTTC 1 cut(s) 120
BstBI TTCGAA 1 cut(s) 109
BstDEI CTNAG 1 cut(s) 137
BstDSI CCRYGG 1 cut(s) 22
BstF5I GGATG 1 cut(s) 52
BstKTI GATC 2 cut(s) 81, 190
BstMAI GTCTC 1 cut(s) 268
BstMBI GATC 2 cut(s) 78, 187
BstMWI GCNNNNNNNGC 1 cut(s) 249
BstNI CCWGG 1 cut(s) 345
BstSCI CCNGG 1 cut(s) 343
BstSFI CTRYAG 1 cut(s) 52
BstV2I GAAGAC 1 cut(s) 279
BstX2I RGATCY 1 cut(s) 187
BstYI RGATCY 1 cut(s) 187
BtgI CCRYGG 1 cut(s) 22
BtsCI GGATG 1 cut(s) 52
Cfr13I GGNCC 1 cut(s) 130
CseI GACGC 1 cut(s) 241
CviAII CATG 3 cut(s) 23, 147, 301
CviJI RGCY 5 cut(s) 5, 141, 228, 243, 410
CviKI_1 RGCY 5 cut(s) 5, 141, 228, 243, 410
DdeI CTNAG 1 cut(s) 137
DpnI GATC 2 cut(s) 80, 189
DpnII GATC 2 cut(s) 78, 187
Eam1104I CTCTTC 1 cut(s) 120
EarI CTCTTC 1 cut(s) 120
Eco130I CCWWGG 1 cut(s) 22
Eco31I GGTCTC 1 cut(s) 268
Eco47I GGWCC 1 cut(s) 130
Eco57I CTGAAG 1 cut(s) 273
EcoO109I RGGNCCY 1 cut(s) 130
EcoRII CCWGG 1 cut(s) 343
EcoT14I CCWWGG 1 cut(s) 22
EcoT22I ATGCAT 1 cut(s) 148
ErhI CCWWGG 1 cut(s) 22
FaeI CATG 3 cut(s) 26, 150, 304
FalI AAGNNNNNCTT 2 cut(s) 117, 149
FatI CATG 3 cut(s) 22, 146, 300
FblI GTMKAC 1 cut(s) 51
FokI GGATG 1 cut(s) 59
FspBI CTAG 1 cut(s) 436
HgaI GACGC 1 cut(s) 241
Hin1II CATG 3 cut(s) 26, 150, 304
HincII GTYRAC 1 cut(s) 198
HindII GTYRAC 1 cut(s) 198
HinfI GANTC 3 cut(s) 371, 395, 421
HphI GGTGA 2 cut(s) 128, 373
Hpy166II GTNNAC 2 cut(s) 52, 198
Hpy188I TCNGA 3 cut(s) 46, 267, 376
Hpy188III TCNNGA 3 cut(s) 8, 74, 82
Hpy8I GTNNAC 2 cut(s) 52, 198
HpyAV CCTTC 1 cut(s) 143
HpyCH4III ACNGT 2 cut(s) 56, 172
HpyCH4V TGCA 2 cut(s) 146, 358
HpyF10VI GCNNNNNNNGC 1 cut(s) 249
HpyF3I CTNAG 1 cut(s) 137
Hsp92II CATG 3 cut(s) 26, 150, 304
Kzo9I GATC 2 cut(s) 78, 187
LmnI GCTCC 1 cut(s) 10
LpnPI CCDG 3 cut(s) 21, 330, 357
MaeI CTAG 1 cut(s) 436
MaeIII GTNAC 1 cut(s) 319
MalI GATC 2 cut(s) 80, 189
MboI GATC 2 cut(s) 78, 187
MboII GAAGA 2 cut(s) 137, 279
MflI RGATCY 1 cut(s) 187
MhlI GDGCHC 1 cut(s) 104
MluCI AATT 2 cut(s) 313, 414
MlyI GAGTC 1 cut(s) 404
MmeI TCCRAC 1 cut(s) 137
MnlI CCTC 3 cut(s) 121, 157, 400
Mph1103I ATGCAT 1 cut(s) 148
MseI TTAA 1 cut(s) 306
MspR9I CCNGG 1 cut(s) 345
MvaI CCWGG 1 cut(s) 345
MwoI GCNNNNNNNGC 1 cut(s) 249
NcoI CCATGG 1 cut(s) 22
NdeII GATC 2 cut(s) 78, 187
NlaIII CATG 3 cut(s) 26, 150, 304
NlaIV GGNNCC 1 cut(s) 6
NsiI ATGCAT 1 cut(s) 148
NspV TTCGAA 1 cut(s) 109
PfeI GAWTC 2 cut(s) 371, 421
PflFI GACNNNGTC 1 cut(s) 394
PleI GAGTC 1 cut(s) 403
PpsI GAGTC 1 cut(s) 403
PpuMI RGGWCCY 1 cut(s) 130
Psp5II RGGWCCY 1 cut(s) 130
Psp6I CCWGG 1 cut(s) 343
PspGI CCWGG 1 cut(s) 343
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 1 cut(s) 130
PspPPI RGGWCCY 1 cut(s) 130
PsuI RGATCY 1 cut(s) 187
PsyI GACNNNGTC 1 cut(s) 394
SaqAI TTAA 1 cut(s) 306
Sau3AI GATC 2 cut(s) 78, 187
Sau96I GGNCC 1 cut(s) 130
SchI GAGTC 1 cut(s) 404
ScrFI CCNGG 1 cut(s) 345
SduI GDGCHC 1 cut(s) 104
SetI ASST 4 cut(s) 135, 143, 230, 346
SfcI CTRYAG 1 cut(s) 52
SfuI TTCGAA 1 cut(s) 109
SinI GGWCC 1 cut(s) 130
SmlI CTYRAG 1 cut(s) 74
SmoI CTYRAG 1 cut(s) 74
Sse9I AATT 2 cut(s) 313, 414
SspMI CTAG 1 cut(s) 436
StyD4I CCNGG 1 cut(s) 343
StyI CCWWGG 1 cut(s) 22
TaaI ACNGT 2 cut(s) 56, 172
TaqI TCGA 1 cut(s) 109
TasI AATT 2 cut(s) 313, 414
TfiI GAWTC 2 cut(s) 371, 421
Tru1I TTAA 1 cut(s) 306
Tru9I TTAA 1 cut(s) 306
TspDTI ATGAA 3 cut(s) 138, 164, 384
Tth111I GACNNNGTC 1 cut(s) 394
VpaK11BI GGWCC 1 cut(s) 130
XmiI GTMKAC 1 cut(s) 51
XspI CTAG 1 cut(s) 436
Zsp2I ATGCAT 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.