pycom11g00870

DNA (cytosine-5)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0001701
Physical Location & Seq
Reverse (-)
659703 .. 662184
2482 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 522 bp
ATGCGTGCGTTTAACAGTGGAACAGTTGGCATTGTAAACAAGTCGTTCATATCTTCGATGAGAAAGGGAGAGACGAAAGAAGAACCCTACATTTGTAAGATTGTGGAAATGTTTGAAGCAATTGGTGGGCTATTATATTTTACTGCACAACGGTACTATAGGTCACGAGACACTCACTGTGCTAGTGTTGCTTGTGGACGTGTATTTGTTTCAGACGTCCGAGACGACAACCCTCTGGATTGTCTTGTTGAAAAACTTCATATTGTTAGGCTGACACTAAATGTTGATCCAGATGCGAAGAGTAAATCAATTCCAGTGTGCGATTACTATTGCGACACCAAAAAATATAGTCTGGAAGCGATGACTCAACTATCGCTGTTGAGGATGATGTTTTGTTTGGATAGTGAGGTTGATTCAAAGCTGCCAAATGGAGTGTGTACGAAATCAGAAGTAAGGCTCCTGGACCTATATTTGGGCGTTGTGGAGCGATGTCTACTGGCCTGTGGCTTGGTGCGCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko01100 ko05206 map00270 map01100 map05206
Pfam Domains
Protein Families
BAH Chromo DNA_methylase

Protein Analysis

174

Amino Acids

19.53

Weight (kDa)

7.46

Isoelectric Point (pI)

30.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BAH PF01426 23 - 120 4.4e-07 BAH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 219
Acc16I TGCGCA 1 cut(s) 515
AccI GTMKAC 1 cut(s) 493
AclWI GGATC 1 cut(s) 281
AcyI GRCGYC 1 cut(s) 216
AfaI GTAC 2 cut(s) 155, 439
AfiI CCNNNNNNNGG 1 cut(s) 472
AflIII ACRYGT 1 cut(s) 199
AgsI TTSAA 3 cut(s) 116, 251, 417
AjiI CACGTC 1 cut(s) 200
AjnI CCWGG 1 cut(s) 459
AluBI AGCT 1 cut(s) 421
AluI AGCT 1 cut(s) 421
Alw26I GTCTC 3 cut(s) 65, 162, 216
AlwI GGATC 1 cut(s) 281
AoxI GGCC 1 cut(s) 498
ApeKI GCWGC 1 cut(s) 421
Asp700I GAANNNNTTC 1 cut(s) 255
AspLEI GCGC 1 cut(s) 516
AspS9I GGNCC 1 cut(s) 463
AvaII GGWCC 1 cut(s) 463
BauI CACGAG 1 cut(s) 165
BbvI GCAGC 1 cut(s) 408
BciT130I CCWGG 1 cut(s) 461
BcoDI GTCTC 3 cut(s) 65, 162, 216
BfaI CTAG 1 cut(s) 183
BfmI CTRYAG 1 cut(s) 157
BisI GCNGC 1 cut(s) 422
BlsI GCNGC 1 cut(s) 423
Bme1390I CCNGG 1 cut(s) 461
Bme18I GGWCC 1 cut(s) 463
BmgBI CACGTC 1 cut(s) 200
BmgT120I GGNCC 1 cut(s) 463
BmiI GGNNCC 1 cut(s) 458
BmrFI CCNGG 1 cut(s) 461
BmsI GCATC 1 cut(s) 283
BsaHI GRCGYC 1 cut(s) 216
Bsc4I CCNNNNNNNGG 1 cut(s) 472
Bse1I ACTGG 2 cut(s) 314, 501
BseBI CCWGG 1 cut(s) 461
BseGI GGATG 1 cut(s) 390
BseLI CCNNNNNNNGG 1 cut(s) 472
BseNI ACTGG 2 cut(s) 314, 501
BseXI GCAGC 1 cut(s) 408
BsgI GTGCAG 1 cut(s) 129
BshFI GGCC 1 cut(s) 500
BslI CCNNNNNNNGG 1 cut(s) 472
BsmAI GTCTC 3 cut(s) 65, 162, 216
BsmBI CGTCTC 2 cut(s) 65, 216
BsnI GGCC 1 cut(s) 500
Bsp143I GATC 1 cut(s) 286
BspANI GGCC 1 cut(s) 500
BspLI GGNNCC 1 cut(s) 458
BspPI GGATC 1 cut(s) 281
BsrI ACTGG 2 cut(s) 314, 501
BssMI GATC 1 cut(s) 286
BssNI GRCGYC 1 cut(s) 216
BssSI CACGAG 1 cut(s) 165
Bst2BI CACGAG 1 cut(s) 165
Bst2UI CCWGG 1 cut(s) 461
Bst4CI ACNGT 4 cut(s) 17, 25, 153, 179
Bst6I CTCTTC 1 cut(s) 293
BstACI GRCGYC 1 cut(s) 216
BstC8I GCNNGC 1 cut(s) 6
BstF5I GGATG 1 cut(s) 390
BstHHI GCGC 1 cut(s) 516
BstKTI GATC 1 cut(s) 289
BstMAI GTCTC 3 cut(s) 65, 162, 216
BstMBI GATC 1 cut(s) 286
BstMWI GCNNNNNNNGC 2 cut(s) 188, 513
BstNI CCWGG 1 cut(s) 461
BstSCI CCNGG 1 cut(s) 459
BstSFI CTRYAG 1 cut(s) 157
BstV1I GCAGC 1 cut(s) 408
BsuRI GGCC 1 cut(s) 500
BtgZI GCGATG 2 cut(s) 374, 502
BtrI CACGTC 1 cut(s) 200
BtsCI GGATG 1 cut(s) 390
BtsIMutI CAGTG 3 cut(s) 22, 175, 321
Cac8I GCNNGC 1 cut(s) 6
CfoI GCGC 1 cut(s) 516
Cfr13I GGNCC 1 cut(s) 463
Csp6I GTAC 2 cut(s) 154, 438
CviJI RGCY 6 cut(s) 130, 271, 421, 457, 500, 507
CviKI_1 RGCY 6 cut(s) 130, 271, 421, 457, 500, 507
CviQI GTAC 2 cut(s) 154, 438
DpnI GATC 1 cut(s) 288
DpnII GATC 1 cut(s) 286
Eam1104I CTCTTC 1 cut(s) 293
EarI CTCTTC 1 cut(s) 293
Eco47I GGWCC 1 cut(s) 463
EcoRII CCWGG 1 cut(s) 459
Esp3I CGTCTC 2 cut(s) 65, 216
FaiI YATR 6 cut(s) 50, 136, 159, 261, 348, 469
FblI GTMKAC 1 cut(s) 493
Fnu4HI GCNGC 1 cut(s) 422
FokI GGATG 1 cut(s) 397
Fsp4HI GCNGC 1 cut(s) 422
FspAI RTGCGCAY 1 cut(s) 515
FspBI CTAG 1 cut(s) 183
FspI TGCGCA 1 cut(s) 515
GlaI GCGC 1 cut(s) 515
GluI GCNGC 1 cut(s) 422
HaeIII GGCC 1 cut(s) 500
HhaI GCGC 1 cut(s) 516
Hin1I GRCGYC 1 cut(s) 216
Hin6I GCGC 1 cut(s) 514
HinP1I GCGC 1 cut(s) 514
HinfI GANTC 2 cut(s) 364, 413
Hpy166II GTNNAC 4 cut(s) 37, 197, 438, 494
Hpy188I TCNGA 3 cut(s) 214, 221, 448
Hpy188III TCNNGA 4 cut(s) 165, 236, 290, 353
Hpy8I GTNNAC 4 cut(s) 37, 197, 438, 494
HpyCH4III ACNGT 4 cut(s) 17, 25, 153, 179
HpyCH4IV ACGT 2 cut(s) 199, 216
HpyCH4V TGCA 1 cut(s) 146
HpyF10VI GCNNNNNNNGC 2 cut(s) 188, 513
HpySE526I ACGT 2 cut(s) 199, 216
Hsp92I GRCGYC 1 cut(s) 216
HspAI GCGC 1 cut(s) 514
Kzo9I GATC 1 cut(s) 286
LmnI GCTCC 2 cut(s) 462, 484
LpnPI CCDG 8 cut(s) 221, 303, 327, 338, 446, 473, 482, 514
Lsp1109I GCAGC 1 cut(s) 408
LweI GCATC 1 cut(s) 283
MaeI CTAG 1 cut(s) 183
MaeII ACGT 2 cut(s) 199, 216
MaeIII GTNAC 1 cut(s) 162
MalI GATC 1 cut(s) 288
MboI GATC 1 cut(s) 286
MboII GAAGA 3 cut(s) 45, 92, 310
MfeI CAATTG 1 cut(s) 120
MluCI AATT 2 cut(s) 120, 309
MlyI GAGTC 1 cut(s) 358
MnlI CCTC 3 cut(s) 243, 375, 400
MroXI GAANNNNTTC 1 cut(s) 255
MseI TTAA 1 cut(s) 12
MspR9I CCNGG 1 cut(s) 461
MunI CAATTG 1 cut(s) 120
MvaI CCWGG 1 cut(s) 461
MwoI GCNNNNNNNGC 2 cut(s) 188, 513
NdeII GATC 1 cut(s) 286
NlaIV GGNNCC 1 cut(s) 458
NmuCI GTSAC 1 cut(s) 162
NsbI TGCGCA 1 cut(s) 515
PcsI WCGNNNNNNNCGW 1 cut(s) 222
PdmI GAANNNNTTC 1 cut(s) 255
PfeI GAWTC 1 cut(s) 413
PfoI TCCNGGA 1 cut(s) 459
PkrI GCNGC 1 cut(s) 423
PleI GAGTC 1 cut(s) 358
PpsI GAGTC 1 cut(s) 358
Psp6I CCWGG 1 cut(s) 459
PspGI CCWGG 1 cut(s) 459
PspN4I GGNNCC 1 cut(s) 458
PspPI GGNCC 1 cut(s) 463
RsaI GTAC 2 cut(s) 155, 439
RsaNI GTAC 2 cut(s) 154, 438
SaqAI TTAA 1 cut(s) 12
SatI GCNGC 1 cut(s) 422
Sau3AI GATC 1 cut(s) 286
Sau96I GGNCC 1 cut(s) 463
SchI GAGTC 1 cut(s) 358
ScrFI CCNGG 1 cut(s) 461
SetI ASST 6 cut(s) 164, 202, 219, 411, 423, 468
SfaNI GCATC 1 cut(s) 283
SfcI CTRYAG 1 cut(s) 157
SinI GGWCC 1 cut(s) 463
Sse9I AATT 2 cut(s) 120, 309
SspMI CTAG 1 cut(s) 183
StyD4I CCNGG 1 cut(s) 459
TaaI ACNGT 4 cut(s) 17, 25, 153, 179
TaiI ACGT 2 cut(s) 202, 219
TaqI TCGA 1 cut(s) 56
TasI AATT 2 cut(s) 120, 309
TfiI GAWTC 1 cut(s) 413
Tru1I TTAA 1 cut(s) 12
Tru9I TTAA 1 cut(s) 12
TscAI CASTG 3 cut(s) 22, 182, 321
TseFI GTSAC 1 cut(s) 162
TseI GCWGC 1 cut(s) 421
Tsp45I GTSAC 1 cut(s) 162
TspDTI ATGAA 2 cut(s) 37, 248
TspRI CASTG 3 cut(s) 22, 182, 321
VpaK11BI GGWCC 1 cut(s) 463
XmiI GTMKAC 1 cut(s) 493
XmnI GAANNNNTTC 1 cut(s) 255
XspI CTAG 1 cut(s) 183
ZraI GACGTC 1 cut(s) 217
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.