pycom11g05010

Nodulin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0001224
Physical Location & Seq
Forward (+)
3895044 .. 3897639
2596 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1626 bp
ATGTCATCATCATCATCTTCTTCTTCTTCTTCTTCTTCTTCTTCTGCTGCTCTCCAATGGCTAAGCCTTGTGGGAATCATATGGCTTCAGTCCATCAATGGAACGAACACCAACTTCCCAGCTTACTCCTCCCAGCTCAAAAACGTCCTCTCCATGTCCCAACTACAGCTCAATAACCTCGCCTTCGCCTCAGATGCCGGAAAACTCCTCGGATGGTTTTCCGGCATTGCTGCCATCCACTTACCCCTGTGGGTAGTCCTCATGATCGGTTCGCTACTGGGGTTTATCGGGTATGGCGTCCAGTTCCTCTTCATCACAAACCAAATCCCATCGCCATCCTATTGGCAGATTTTCCTGTTAACTGTTATGGCTGGAAATAGCATTTGCTGGATCAACACTGTGTGCTATGTTGTCAGCATTAGAAACTTCCCATGTCATCAACAAATTGCCGTGGGGATAACAACAAGTTACCAAGGATTGAGCGCCAAGATTTATACAGATATTGTTGGCGCTGCATTTTCGTCTTCGCCTCACAGAAGAGCCAAGGCCTACTTACTTCTCAACTCTCTCTTGCCACTTGTTGTGTGTGTTATCGTCGCGCCATTGGTTAGAGACATTGATCTGATGGGAAGGCAAAGTAACATGGAAGCCGGGTTTGTTTTTATGTTCGTCATAACAATTGCTACAGGAATCTACGCGGTGATTAGCAGTTTAGGGTCGATTTCTAGCGGGTTCTCTCCTTTGCATAATGTAATAGGAACCGGATTGTTCCTATTAGTCCCATTAGTAATCCCGGTTGTGGATAAAATGAGAGAAGTATTTTCAAAAAAATGGAACATAAAAAGAGAGAGAAGAGTGTATAATTTTACCATAGATGAGAAGAACAACGAAACGGGCAGCTTAGAGAATGTGGGAGTGAAAGAGGGAGAAGAATTAGATGCTAGACAAGTTCTTCAAGTTGGTTGTAGGGAGGAGATTGGAGTGAAGTTGATGGTGACAAGGATTGATTTTTGGTTATATTTCTTTGTTTATTTGTTCAGTGCAACACTTGGGTTGGTGTTCTTGAACAACTTGGGACAGATTTCAGAGTCCCGGGGATCGTCTAGGACTTCTTCTTTGGTCTCTTTATCCTCTTCATTTGGGTTTTTTGGCCGTCTCATGCCTTCTCTTTTGGACTACTTCTTCTCAAGGAGTAAGTATATGATTTCAAGGCCAGCATTAGTAGTTGCATTAATGACACCAATAGCAGGAGCCTTCTTCTTGCTTCTCAACCCAGCCAACCTCTCACTTTTCATCAGCACAGCAATTATAGGAGTCTGTACTGGAGCTATTACTTCAATCGCAGTGTCCATAACCACAGAGCTGTTTGGGACCAAGAGTTTCTCAGTGAACCACAATGTGGTGGTGGCCAACATATCAATCGGGTCTTTTGTGTTTGGATACTTGTCTGCTATTCTTTACCGCAAAGAAGGAAATCATGACGGTGACGGGAAGTGCATGGGAATGGCTTGCTACAGAAGCACATTTATTATATGGGGCTGCCTTTGTTTTCTGGGAACTGGCTTAGCTTTAATGCTCTATGCACGAACACGAAAGTTCTACTCCCAAAGATCATTAAATATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nodulin-like

Protein Analysis

542

Amino Acids

59.23

Weight (kDa)

9.28

Isoelectric Point (pI)

43.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nodulin-like PF06813 19 - 266 1.4e-75 Nodulin-like
NFD4_C PF23262 320 - 535 1.7e-102 NFD4, C-terminal domain
MFS_1 PF07690 405 - 532 8.4e-06 Major Facilitator Superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1399
AccII CGCG 2 cut(s) 599, 698
AciI CCGC 3 cut(s) 698, 729, 1462
AclWI GGATC 2 cut(s) 398, 1105
AcoI YGGCCR 2 cut(s) 1150, 1407
AcuI CTGAAG 1 cut(s) 71
AcyI GRCGYC 1 cut(s) 297
AdeI CACNNNGTG 2 cut(s) 402, 1399
AfaI GTAC 1 cut(s) 1321
AfiI CCNNNNNNNGG 3 cut(s) 799, 1247, 1399
AgsI TTSAA 5 cut(s) 825, 956, 1066, 1209, 1338
AluBI AGCT 7 cut(s) 122, 136, 169, 900, 1328, 1363, 1568
AluI AGCT 7 cut(s) 122, 136, 169, 900, 1328, 1363, 1568
Alw26I GTCTC 3 cut(s) 606, 1126, 1160
AlwI GGATC 2 cut(s) 398, 1105
Ama87I CYCGRG 1 cut(s) 1092
AoxI GGCC 4 cut(s) 546, 1150, 1211, 1407
ApeKI GCWGC 5 cut(s) 47, 230, 512, 897, 1539
ArsI GACNNNNNNTTYG 2 cut(s) 1099, 1131
AseI ATTAAT 1 cut(s) 1232
AspLEI GCGC 3 cut(s) 485, 512, 601
AspS9I GGNCC 1 cut(s) 1371
AsuC2I CCSGG 4 cut(s) 652, 794, 1093, 1094
AsuHPI GGTGA 3 cut(s) 712, 1006, 1496
AvaI CYCGRG 1 cut(s) 1092
AvaII GGWCC 1 cut(s) 1371
BalI TGGCCA 1 cut(s) 1409
BbsI GAAGAC 1 cut(s) 516
BbvI GCAGC 5 cut(s) 34, 217, 499, 909, 1526
BccI CCATC 7 cut(s) 101, 207, 242, 337, 343, 619, 985
BceAI ACGGC 2 cut(s) 434, 1137
BciVI GTATCC 1 cut(s) 1433
BcnI CCSGG 4 cut(s) 652, 794, 1093, 1094
BcoDI GTCTC 3 cut(s) 606, 1126, 1160
BfaI CTAG 3 cut(s) 726, 942, 1104
BfmI CTRYAG 3 cut(s) 164, 684, 1513
BfoI RGCGCY 2 cut(s) 486, 513
BfuI GTATCC 1 cut(s) 1433
BisI GCNGC 5 cut(s) 48, 231, 513, 898, 1540
BlpI GCTNAGC 2 cut(s) 62, 1564
BlsI GCNGC 5 cut(s) 49, 232, 514, 899, 1541
Bme1390I CCNGG 4 cut(s) 652, 794, 1093, 1094
Bme18I GGWCC 1 cut(s) 1371
BmeT110I CYCGRG 1 cut(s) 1092
BmgT120I GGNCC 1 cut(s) 1371
BmiI GGNNCC 3 cut(s) 760, 1252, 1372
BmrFI CCNGG 4 cut(s) 652, 794, 1093, 1094
BmrI ACTGGG 1 cut(s) 287
BmsI GCATC 2 cut(s) 184, 928
BmuI ACTGGG 1 cut(s) 287
BpiI GAAGAC 1 cut(s) 516
BpmI CTGGAG 1 cut(s) 1344
Bpu1102I GCTNAGC 2 cut(s) 62, 1564
BpuEI CTTGAG 1 cut(s) 1171
BpuMI CCSGG 4 cut(s) 652, 794, 1093, 1094
BsaHI GRCGYC 1 cut(s) 297
BsaI GGTCTC 1 cut(s) 1126
BsaJI CCNNGG 6 cut(s) 208, 450, 472, 543, 1092, 1093
BsaWI WCCGGW 1 cut(s) 761
BsaXI ACNNNNNCTCC 2 cut(s) 134, 164
Bsc4I CCNNNNNNNGG 3 cut(s) 799, 1247, 1399
Bse1I ACTGG 4 cut(s) 282, 301, 1327, 1564
Bse3DI GCAATG 1 cut(s) 225
BseDI CCNNGG 6 cut(s) 208, 450, 472, 543, 1092, 1093
BseGI GGATG 3 cut(s) 218, 234, 335
BseLI CCNNNNNNNGG 3 cut(s) 799, 1247, 1399
BseMI GCAATG 1 cut(s) 225
BseMII CTCAG 2 cut(s) 204, 1398
BseNI ACTGG 4 cut(s) 282, 301, 1327, 1564
BseRI GAGGAG 3 cut(s) 118, 197, 986
BseXI GCAGC 5 cut(s) 34, 217, 499, 909, 1526
BseYI CCCAGC 3 cut(s) 118, 132, 1273
Bsh1236I CGCG 2 cut(s) 599, 698
BshFI GGCC 4 cut(s) 548, 1152, 1213, 1409
BsiHKCI CYCGRG 1 cut(s) 1092
BsiSI CCGG 6 cut(s) 198, 222, 651, 762, 794, 1093
BslFI GGGAC 5 cut(s) 142, 764, 1075, 1089, 1384
BslI CCNNNNNNNGG 3 cut(s) 799, 1247, 1399
BsmAI GTCTC 3 cut(s) 606, 1126, 1160
BsmBI CGTCTC 1 cut(s) 1160
BsmFI GGGAC 5 cut(s) 142, 764, 1075, 1089, 1384
BsnI GGCC 4 cut(s) 548, 1152, 1213, 1409
Bso31I GGTCTC 1 cut(s) 1126
BsoBI CYCGRG 1 cut(s) 1092
Bsp143I GATC 5 cut(s) 264, 390, 619, 1097, 1610
Bsp1720I GCTNAGC 2 cut(s) 62, 1564
BspACI CCGC 3 cut(s) 698, 729, 1462
BspANI GGCC 4 cut(s) 548, 1152, 1213, 1409
BspCNI CTCAG 2 cut(s) 203, 1397
BspFNI CGCG 2 cut(s) 599, 698
BspHI TCATGA 2 cut(s) 261, 1477
BspLI GGNNCC 3 cut(s) 760, 1252, 1372
BspPI GGATC 2 cut(s) 398, 1105
BspQI GCTCTTC 1 cut(s) 532
BspTNI GGTCTC 1 cut(s) 1126
BsrDI GCAATG 1 cut(s) 225
BsrI ACTGG 4 cut(s) 282, 301, 1327, 1564
BssECI CCNNGG 6 cut(s) 208, 450, 472, 543, 1092, 1093
BssMI GATC 5 cut(s) 264, 390, 619, 1097, 1610
BssNI GRCGYC 1 cut(s) 297
BssT1I CCWWGG 2 cut(s) 472, 543
Bst4CI ACNGT 3 cut(s) 364, 400, 1484
Bst6I CTCTTC 4 cut(s) 314, 532, 847, 1138
BstACI GRCGYC 1 cut(s) 297
BstC8I GCNNGC 2 cut(s) 1215, 1510
BstDEI CTNAG 5 cut(s) 62, 190, 901, 1384, 1564
BstDSI CCRYGG 1 cut(s) 450
BstF5I GGATG 3 cut(s) 218, 234, 335
BstFNI CGCG 2 cut(s) 599, 698
BstH2I RGCGCY 2 cut(s) 486, 513
BstHHI GCGC 3 cut(s) 485, 512, 601
BstKTI GATC 5 cut(s) 267, 393, 622, 1100, 1613
BstMAI GTCTC 3 cut(s) 606, 1126, 1160
BstMBI GATC 5 cut(s) 264, 390, 619, 1097, 1610
BstMWI GCNNNNNNNGC 2 cut(s) 194, 1518
BstSCI CCNGG 4 cut(s) 650, 792, 1091, 1092
BstSFI CTRYAG 3 cut(s) 164, 684, 1513
BstUI CGCG 2 cut(s) 599, 698
BstV1I GCAGC 5 cut(s) 34, 217, 499, 909, 1526
BstV2I GAAGAC 1 cut(s) 516
BstXI CCANNNNNNTGG 1 cut(s) 342
BsuI GTATCC 1 cut(s) 1433
BsuRI GGCC 4 cut(s) 548, 1152, 1213, 1409
BtgI CCRYGG 1 cut(s) 450
BtgZI GCGATG 1 cut(s) 315
BtsCI GGATG 3 cut(s) 218, 234, 335
BtsI GCAGTG 1 cut(s) 1350
BtsIMutI CAGTG 4 cut(s) 396, 1045, 1350, 1392
Cac8I GCNNGC 2 cut(s) 1215, 1510
CciI TCATGA 2 cut(s) 261, 1477
CfoI GCGC 3 cut(s) 485, 512, 601
Cfr13I GGNCC 1 cut(s) 1371
Cfr9I CCCGGG 1 cut(s) 1092
CseI GACGC 1 cut(s) 286
Csp6I GTAC 1 cut(s) 1320
CviAII CATG 7 cut(s) 154, 262, 432, 643, 1159, 1478, 1498
CviQI GTAC 1 cut(s) 1320
DdeI CTNAG 5 cut(s) 62, 190, 901, 1384, 1564
DpnI GATC 5 cut(s) 266, 392, 621, 1099, 1612
DpnII GATC 5 cut(s) 264, 390, 619, 1097, 1610
DraIII CACNNNGTG 2 cut(s) 402, 1399
EaeI YGGCCR 2 cut(s) 1150, 1407
Eam1104I CTCTTC 4 cut(s) 314, 532, 847, 1138
EarI CTCTTC 4 cut(s) 314, 532, 847, 1138
Eco130I CCWWGG 2 cut(s) 472, 543
Eco147I AGGCCT 1 cut(s) 548
Eco31I GGTCTC 1 cut(s) 1126
Eco47I GGWCC 1 cut(s) 1371
Eco57I CTGAAG 1 cut(s) 71
Eco88I CYCGRG 1 cut(s) 1092
EcoT14I CCWWGG 2 cut(s) 472, 543
ErhI CCWWGG 2 cut(s) 472, 543
Esp3I CGTCTC 1 cut(s) 1160
FaeI CATG 7 cut(s) 157, 265, 435, 646, 1162, 1481, 1501
FalI AAGNNNNNCTT 2 cut(s) 536, 568
FaqI GGGAC 5 cut(s) 142, 764, 1075, 1089, 1384
FatI CATG 7 cut(s) 153, 261, 431, 642, 1158, 1477, 1497
FauI CCCGC 1 cut(s) 722
FauNDI CATATG 1 cut(s) 80
Fnu4HI GCNGC 5 cut(s) 48, 231, 513, 898, 1540
FokI GGATG 3 cut(s) 221, 225, 322
Fsp4HI GCNGC 5 cut(s) 48, 231, 513, 898, 1540
FspBI CTAG 3 cut(s) 726, 942, 1104
GlaI GCGC 3 cut(s) 484, 511, 600
GluI GCNGC 5 cut(s) 48, 231, 513, 898, 1540
GsaI CCCAGC 3 cut(s) 122, 136, 1277
GsuI CTGGAG 1 cut(s) 1344
HaeII RGCGCY 2 cut(s) 486, 513
HaeIII GGCC 4 cut(s) 548, 1152, 1213, 1409
HapII CCGG 6 cut(s) 198, 222, 651, 762, 794, 1093
HgaI GACGC 1 cut(s) 286
HhaI GCGC 3 cut(s) 485, 512, 601
Hin1I GRCGYC 1 cut(s) 297
Hin1II CATG 7 cut(s) 157, 265, 435, 646, 1162, 1481, 1501
Hin6I GCGC 3 cut(s) 483, 510, 599
HinP1I GCGC 3 cut(s) 483, 510, 599
HincII GTYRAC 1 cut(s) 360
HindII GTYRAC 1 cut(s) 360
HinfI GANTC 4 cut(s) 75, 690, 1088, 1314
HpaI GTTAAC 1 cut(s) 360
HpaII CCGG 6 cut(s) 198, 222, 651, 762, 794, 1093
HphI GGTGA 3 cut(s) 712, 1006, 1496
Hpy166II GTNNAC 2 cut(s) 360, 1390
Hpy188I TCNGA 4 cut(s) 193, 212, 624, 1087
Hpy188III TCNNGA 3 cut(s) 262, 1063, 1478
Hpy8I GTNNAC 2 cut(s) 360, 1390
Hpy99I CGWCG 1 cut(s) 599
HpyAV CCTTC 5 cut(s) 193, 624, 1173, 1264, 1463
HpyCH4III ACNGT 3 cut(s) 364, 400, 1484
HpyCH4IV ACGT 1 cut(s) 144
HpyCH4V TGCA 6 cut(s) 515, 745, 1043, 1229, 1497, 1583
HpyF10VI GCNNNNNNNGC 2 cut(s) 194, 1518
HpyF3I CTNAG 5 cut(s) 62, 190, 901, 1384, 1564
HpySE526I ACGT 1 cut(s) 144
Hsp92I GRCGYC 1 cut(s) 297
Hsp92II CATG 7 cut(s) 157, 265, 435, 646, 1162, 1481, 1501
HspAI GCGC 3 cut(s) 483, 510, 599
KspAI GTTAAC 1 cut(s) 360
Kzo9I GATC 5 cut(s) 264, 390, 619, 1097, 1610
LguI GCTCTTC 1 cut(s) 532
LmnI GCTCC 2 cut(s) 1250, 1325
Lsp1109I GCAGC 5 cut(s) 34, 217, 499, 909, 1526
LweI GCATC 2 cut(s) 184, 928
MaeI CTAG 3 cut(s) 726, 942, 1104
MaeII ACGT 1 cut(s) 144
MaeIII GTNAC 4 cut(s) 467, 638, 994, 1484
MalI GATC 5 cut(s) 266, 392, 621, 1099, 1612
MboI GATC 5 cut(s) 264, 390, 619, 1097, 1610
MfeI CAATTG 1 cut(s) 678
MlsI TGGCCA 1 cut(s) 1409
MluCI AATT 5 cut(s) 444, 678, 862, 932, 1305
MluNI TGGCCA 1 cut(s) 1409
MlyI GAGTC 2 cut(s) 1097, 1323
Mox20I TGGCCA 1 cut(s) 1409
MscI TGGCCA 1 cut(s) 1409
MseI TTAA 4 cut(s) 359, 1232, 1571, 1616
MslI CAYNNNNRTG 2 cut(s) 1482, 1502
Msp20I TGGCCA 1 cut(s) 1409
MspI CCGG 6 cut(s) 198, 222, 651, 762, 794, 1093
MspR9I CCNGG 4 cut(s) 652, 794, 1093, 1094
MunI CAATTG 1 cut(s) 678
MvnI CGCG 2 cut(s) 599, 698
MwoI GCNNNNNNNGC 2 cut(s) 194, 1518
NciI CCSGG 4 cut(s) 652, 794, 1093, 1094
NdeI CATATG 1 cut(s) 80
NdeII GATC 5 cut(s) 264, 390, 619, 1097, 1610
NlaIII CATG 7 cut(s) 157, 265, 435, 646, 1162, 1481, 1501
NlaIV GGNNCC 3 cut(s) 760, 1252, 1372
NmuCI GTSAC 2 cut(s) 994, 1484
PagI TCATGA 2 cut(s) 261, 1477
PceI AGGCCT 1 cut(s) 548
PciSI GCTCTTC 1 cut(s) 532
PcsI WCGNNNNNNNCGW 1 cut(s) 294
PfeI GAWTC 2 cut(s) 75, 690
PflMI CCANNNNNTGG 1 cut(s) 1399
PkrI GCNGC 5 cut(s) 49, 232, 514, 899, 1541
PleI GAGTC 2 cut(s) 1096, 1322
PpsI GAGTC 2 cut(s) 1096, 1322
PshBI ATTAAT 1 cut(s) 1232
PspFI CCCAGC 3 cut(s) 118, 132, 1273
PspN4I GGNNCC 3 cut(s) 760, 1252, 1372
PspPI GGNCC 1 cut(s) 1371
RsaI GTAC 1 cut(s) 1321
RsaNI GTAC 1 cut(s) 1320
RseI CAYNNNNRTG 2 cut(s) 1482, 1502
SapI GCTCTTC 1 cut(s) 532
SaqAI TTAA 4 cut(s) 359, 1232, 1571, 1616
SatI GCNGC 5 cut(s) 48, 231, 513, 898, 1540
Sau3AI GATC 5 cut(s) 264, 390, 619, 1097, 1610
Sau96I GGNCC 1 cut(s) 1371
SchI GAGTC 2 cut(s) 1097, 1323
ScrFI CCNGG 4 cut(s) 652, 794, 1093, 1094
SfaNI GCATC 2 cut(s) 184, 928
SfcI CTRYAG 3 cut(s) 164, 684, 1513
SinI GGWCC 1 cut(s) 1371
SmaI CCCGGG 1 cut(s) 1094
SmiMI CAYNNNNRTG 2 cut(s) 1482, 1502
SmlI CTYRAG 1 cut(s) 1186
SmoI CTYRAG 1 cut(s) 1186
Sse9I AATT 5 cut(s) 444, 678, 862, 932, 1305
SseBI AGGCCT 1 cut(s) 548
SsiI CCGC 3 cut(s) 698, 729, 1462
SspMI CTAG 3 cut(s) 726, 942, 1104
StuI AGGCCT 1 cut(s) 548
StyD4I CCNGG 4 cut(s) 650, 792, 1091, 1092
StyI CCWWGG 2 cut(s) 472, 543
TaaI ACNGT 3 cut(s) 364, 400, 1484
TaiI ACGT 1 cut(s) 147
TaqI TCGA 1 cut(s) 719
TasI AATT 5 cut(s) 444, 678, 862, 932, 1305
TatI WGTACW 1 cut(s) 1319
TfiI GAWTC 2 cut(s) 75, 690
Tru1I TTAA 4 cut(s) 359, 1232, 1571, 1616
Tru9I TTAA 4 cut(s) 359, 1232, 1571, 1616
TscAI CASTG 4 cut(s) 403, 1045, 1350, 1392
TseFI GTSAC 2 cut(s) 994, 1484
TseI GCWGC 5 cut(s) 47, 230, 512, 897, 1539
Tsp45I GTSAC 2 cut(s) 994, 1484
TspDTI ATGAA 3 cut(s) 301, 1125, 1282
TspMI CCCGGG 1 cut(s) 1092
TspRI CASTG 4 cut(s) 403, 1045, 1350, 1392
Van91I CCANNNNNTGG 1 cut(s) 1399
VpaK11BI GGWCC 1 cut(s) 1371
VspI ATTAAT 1 cut(s) 1232
XmaI CCCGGG 1 cut(s) 1092
XspI CTAG 3 cut(s) 726, 942, 1104
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.